WO2014028311A3 - Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing - Google Patents

Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing Download PDF

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Publication number
WO2014028311A3
WO2014028311A3 PCT/US2013/054250 US2013054250W WO2014028311A3 WO 2014028311 A3 WO2014028311 A3 WO 2014028311A3 US 2013054250 W US2013054250 W US 2013054250W WO 2014028311 A3 WO2014028311 A3 WO 2014028311A3
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WO
WIPO (PCT)
Prior art keywords
cell
polynucleotide
means
binding domains
labeling
Prior art date
Application number
PCT/US2013/054250
Other languages
French (fr)
Other versions
WO2014028311A2 (en
Inventor
George M. Church
Prashant MALI
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201261683690P priority Critical
Priority to US61/683,690 priority
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2014028311A2 publication Critical patent/WO2014028311A2/en
Publication of WO2014028311A3 publication Critical patent/WO2014028311A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Abstract

The present invention relates to a protein comprising a polynucleotide-binding domain and a transmembrane domain. These proteins can be used in methods of sequencing polynucleotides, methods of labeling polynucleotides, and methods of organizing cells (e.g., to construct tissues).
PCT/US2013/054250 2012-08-15 2013-08-09 Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing WO2014028311A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US201261683690P true 2012-08-15 2012-08-15
US61/683,690 2012-08-15

Publications (2)

Publication Number Publication Date
WO2014028311A2 WO2014028311A2 (en) 2014-02-20
WO2014028311A3 true WO2014028311A3 (en) 2015-07-16

Family

ID=50101578

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/054250 WO2014028311A2 (en) 2012-08-15 2013-08-09 Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing

Country Status (1)

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WO (1) WO2014028311A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017167811A1 (en) * 2016-03-31 2017-10-05 Genia Technologies, Inc. Nanopore protein conjugates and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US20070178499A1 (en) * 2006-01-06 2007-08-02 The Scripps Research Institute Specific Labeling of Protein with Zinc Finger Tags and Use of Zinc-Finger-Tagged Proteins for Analysis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US20070178499A1 (en) * 2006-01-06 2007-08-02 The Scripps Research Institute Specific Labeling of Protein with Zinc Finger Tags and Use of Zinc-Finger-Tagged Proteins for Analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GARTNER, Z ET AL.: "Programmed Assembly Of 3-Dimensional Microtissues With Defined Cellular Connectivity.", PNAS., vol. 106, no. 12, 9 March 2009 (2009-03-09), pages 4606 - 4610, XP055039752, DOI: doi:10.1073/pnas.0900717106 *
HUAMBACHANO, O. ET AL.: "Double-Stranded DNA Binding Domain of Poly(ADP-ribose) Polymerase-1 And Molecular Insight Into The Regulation Of Its Activity.", J. BIOL. CHEM., vol. 286, 23 December 2010 (2010-12-23), pages 7149 - 7160 *

Also Published As

Publication number Publication date
WO2014028311A2 (en) 2014-02-20

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