Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing

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Publication number
WO2014028311A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
methods
sequencing
binding
domain
polynucleotide
Prior art date
Application number
PCT/US2013/054250
Other languages
French (fr)
Other versions
WO2014028311A2 (en )
Inventor
George M. Church
Prashant MALI
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Abstract

The present invention relates to a protein comprising a polynucleotide-binding domain and a transmembrane domain. These proteins can be used in methods of sequencing polynucleotides, methods of labeling polynucleotides, and methods of organizing cells (e.g., to construct tissues).

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US20070178499A1 (en) * 2006-01-06 2007-08-02 The Scripps Research Institute Specific Labeling of Protein with Zinc Finger Tags and Use of Zinc-Finger-Tagged Proteins for Analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GARTNER, Z ET AL.: "Programmed Assembly Of 3-Dimensional Microtissues With Defined Cellular Connectivity.", PNAS., vol. 106, no. 12, 9 March 2009 (2009-03-09), pages 4606 - 4610, XP055039752, DOI: doi:10.1073/pnas.0900717106 *
HUAMBACHANO, O. ET AL.: "Double-Stranded DNA Binding Domain of Poly(ADP-ribose) Polymerase-1 And Molecular Insight Into The Regulation Of Its Activity.", J. BIOL. CHEM., vol. 286, 23 December 2010 (2010-12-23), pages 7149 - 7160 *

Also Published As

Publication number Publication date Type
WO2014028311A2 (en) 2014-02-20 application

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