WO2014028311A3 - Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing - Google Patents
Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing Download PDFInfo
- Publication number
- WO2014028311A3 WO2014028311A3 PCT/US2013/054250 US2013054250W WO2014028311A3 WO 2014028311 A3 WO2014028311 A3 WO 2014028311A3 US 2013054250 W US2013054250 W US 2013054250W WO 2014028311 A3 WO2014028311 A3 WO 2014028311A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cell
- polynucleotide
- binding domains
- labeling
- organization
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
- C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
Abstract
The present invention relates to a protein comprising a polynucleotide-binding domain and a transmembrane domain. These proteins can be used in methods of sequencing polynucleotides, methods of labeling polynucleotides, and methods of organizing cells (e.g., to construct tissues).
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261683690P | 2012-08-15 | 2012-08-15 | |
US61/683,690 | 2012-08-15 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2014028311A2 WO2014028311A2 (en) | 2014-02-20 |
WO2014028311A3 true WO2014028311A3 (en) | 2015-07-16 |
Family
ID=50101578
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2013/054250 WO2014028311A2 (en) | 2012-08-15 | 2013-08-09 | Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2014028311A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017167811A1 (en) * | 2016-03-31 | 2017-10-05 | Genia Technologies, Inc. | Nanopore protein conjugates and uses thereof |
JP2022547154A (en) | 2019-09-10 | 2022-11-10 | オブシディアン セラピューティクス, インコーポレイテッド | CA2-IL15 fusion proteins for regulatable control |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7176025B2 (en) * | 2002-03-11 | 2007-02-13 | Nugen Technologies, Inc. | Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination |
US20070178499A1 (en) * | 2006-01-06 | 2007-08-02 | The Scripps Research Institute | Specific Labeling of Protein with Zinc Finger Tags and Use of Zinc-Finger-Tagged Proteins for Analysis |
-
2013
- 2013-08-09 WO PCT/US2013/054250 patent/WO2014028311A2/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7176025B2 (en) * | 2002-03-11 | 2007-02-13 | Nugen Technologies, Inc. | Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination |
US20070178499A1 (en) * | 2006-01-06 | 2007-08-02 | The Scripps Research Institute | Specific Labeling of Protein with Zinc Finger Tags and Use of Zinc-Finger-Tagged Proteins for Analysis |
Non-Patent Citations (2)
Title |
---|
GARTNER, Z ET AL.: "Programmed Assembly Of 3-Dimensional Microtissues With Defined Cellular Connectivity.", PNAS., vol. 106, no. 12, 9 March 2009 (2009-03-09), pages 4606 - 4610 * |
HUAMBACHANO, O. ET AL.: "Double-Stranded DNA Binding Domain of Poly(ADP-ribose) Polymerase-1 And Molecular Insight Into The Regulation Of Its Activity.", J. BIOL. CHEM., vol. 286, 23 December 2010 (2010-12-23), pages 7149 - 7160 * |
Also Published As
Publication number | Publication date |
---|---|
WO2014028311A2 (en) | 2014-02-20 |
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