Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing

Info

Publication number
WO2014028311A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
methods
sequencing
binding
domain
polynucleotide
Prior art date
Application number
PCT/US2013/054250
Other languages
French (fr)
Other versions
WO2014028311A2 (en )
Inventor
George M. Church
Prashant MALI
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Abstract

The present invention relates to a protein comprising a polynucleotide-binding domain and a transmembrane domain. These proteins can be used in methods of sequencing polynucleotides, methods of labeling polynucleotides, and methods of organizing cells (e.g., to construct tissues).

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US20070178499A1 (en) * 2006-01-06 2007-08-02 The Scripps Research Institute Specific Labeling of Protein with Zinc Finger Tags and Use of Zinc-Finger-Tagged Proteins for Analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GARTNER, Z ET AL.: "Programmed Assembly Of 3-Dimensional Microtissues With Defined Cellular Connectivity.", PNAS., vol. 106, no. 12, 9 March 2009 (2009-03-09), pages 4606 - 4610, XP055039752, DOI: doi:10.1073/pnas.0900717106 *
HUAMBACHANO, O. ET AL.: "Double-Stranded DNA Binding Domain of Poly(ADP-ribose) Polymerase-1 And Molecular Insight Into The Regulation Of Its Activity.", J. BIOL. CHEM., vol. 286, 23 December 2010 (2010-12-23), pages 7149 - 7160 *

Also Published As

Publication number Publication date Type
WO2014028311A2 (en) 2014-02-20 application

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