WO2014028311A3 - Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing - Google Patents

Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing Download PDF

Info

Publication number
WO2014028311A3
WO2014028311A3 PCT/US2013/054250 US2013054250W WO2014028311A3 WO 2014028311 A3 WO2014028311 A3 WO 2014028311A3 US 2013054250 W US2013054250 W US 2013054250W WO 2014028311 A3 WO2014028311 A3 WO 2014028311A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
polynucleotide
binding domains
labeling
organization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/054250
Other languages
French (fr)
Other versions
WO2014028311A2 (en
Inventor
George M. Church
Prashant MALI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of WO2014028311A2 publication Critical patent/WO2014028311A2/en
Anticipated expiration legal-status Critical
Publication of WO2014028311A3 publication Critical patent/WO2014028311A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a protein comprising a polynucleotide-binding domain and a transmembrane domain. These proteins can be used in methods of sequencing polynucleotides, methods of labeling polynucleotides, and methods of organizing cells (e.g., to construct tissues).
PCT/US2013/054250 2012-08-15 2013-08-09 Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing Ceased WO2014028311A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261683690P 2012-08-15 2012-08-15
US61/683,690 2012-08-15

Publications (2)

Publication Number Publication Date
WO2014028311A2 WO2014028311A2 (en) 2014-02-20
WO2014028311A3 true WO2014028311A3 (en) 2015-07-16

Family

ID=50101578

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/054250 Ceased WO2014028311A2 (en) 2012-08-15 2013-08-09 Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing

Country Status (1)

Country Link
WO (1) WO2014028311A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3436602B1 (en) 2016-03-31 2024-08-14 Genia Technologies, Inc. Nanopore protein conjugates and uses thereof
EP4028413A1 (en) 2019-09-10 2022-07-20 Obsidian Therapeutics, Inc. Ca2-il15 fusion proteins for tunable regulation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US20070178499A1 (en) * 2006-01-06 2007-08-02 The Scripps Research Institute Specific Labeling of Protein with Zinc Finger Tags and Use of Zinc-Finger-Tagged Proteins for Analysis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US20070178499A1 (en) * 2006-01-06 2007-08-02 The Scripps Research Institute Specific Labeling of Protein with Zinc Finger Tags and Use of Zinc-Finger-Tagged Proteins for Analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GARTNER, Z ET AL.: "Programmed Assembly Of 3-Dimensional Microtissues With Defined Cellular Connectivity.", PNAS., vol. 106, no. 12, 9 March 2009 (2009-03-09), pages 4606 - 4610 *
HUAMBACHANO, O. ET AL.: "Double-Stranded DNA Binding Domain of Poly(ADP-ribose) Polymerase-1 And Molecular Insight Into The Regulation Of Its Activity.", J. BIOL. CHEM., vol. 286, 23 December 2010 (2010-12-23), pages 7149 - 7160 *

Also Published As

Publication number Publication date
WO2014028311A2 (en) 2014-02-20

Similar Documents

Publication Publication Date Title
ZA202308846B (en) Cx3cr1-binding polypeptides
EP4491726A3 (en) Protein enriched microvesicles and methods of making and using the same
MY177065A (en) 4-1bb binding molecules
EP2853597A4 (en) NUCLEIC ACID MOLECULE INDUCING INTERFERING RNA CAPABLE OF PENETRATING IN CELLS AND USE THEREOF
PL2334831T3 (en) Pentacyclic anion salt and use thereof as an electrolyte
IL222961B (en) DNA binding proteins and their uses
CL2013002361A1 (en) Method to measure natural killer cell activity (nk); kit to measure said activity; fusion protein; composition for activating nk cells; polypeptide, oligonucleotide, vector and host cell comprising the protein;
HK1223629A1 (en) Binding molecules for bcma and cd3
EP4509189A3 (en) Anti-fcrn antibodies
SG10201609347RA (en) Cell Culture Medium Comprising Small Peptides
JP2012140442A5 (en)
EP2816141A4 (en) ELECTROLYSIS CELL, AND ELECTROLYSIS TANK
WO2011130624A3 (en) Sustained polypeptide expression from synthetic, modified rnas and uses thereof
EP4282882A3 (en) Binding domains directed against gpcr:g protein complexes and uses derived thereof
PH12013500336A1 (en) Peptide or peptide complex binding to alpha2 integrin and methods and uses involving the same
WO2014100481A8 (en) Modified alpha hemolysin polypeptides and methods of use
GB201111237D0 (en) Nanochip
EP2865041A4 (en) Gel polymer composite electrolyte, polymer lithium ion battery comprising the gel polymer composite electrolyte and methods of preparing the same
WO2015160928A3 (en) Isolated t cell receptors and methods of use therefor
EP3072953A4 (en) Cell culture container and container in which cell culture has been accommodated
EP2691463A4 (en) POLYMER ELECTROLYTE COMPOSITION AND DYE-SENSITIZED SOLAR CELL CONTAINING THE COMPOSITION
WO2012135432A3 (en) Dyes for membranes and biological structures
WO2015120542A8 (en) Hybrid proteins and uses thereof
WO2014028311A3 (en) Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing
WO2012068038A3 (en) Caffeic acid derivatives and their use in improving neuronal cell viability

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13829346

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2013829346

Country of ref document: EP