WO2013174192A1

WO2013174192A1 - Biological sample management system based on two-dimensional bar code technologies

Info

Publication number: WO2013174192A1
Application number: PCT/CN2013/074659
Authority: WO
Inventors: 王俊; 刘小立; 许佳章; 徐健; 于微; 朱李佳; 谭洪兴; 杨慧
Original assignee: 深圳市慢性病防治中心
Priority date: 2012-05-21
Filing date: 2013-04-24
Publication date: 2013-11-28
Also published as: CN102693456A

Abstract

Provided is a biological sample management system based on two-dimensional bar code technologies. The system comprises: a database, a management server connected with the database, and a plurality of management terminals, bar code printers, data collectors and sample tubes connected with the management server through a network. The bar code printer generates a sample tag for each miniature centrifuge tube that stores a biological sample, and generates, according to a storage position of each sample tube in a refrigerator, a two-dimensional bar code for each miniature centrifuge tube that stores a biological sample. The data collector reads the sample tag and the two-dimensional bar code on each sample tube, and collects corresponding biological sample information of the sample tube. The management terminal logs in to the management server, and the management server establishes record data for each sample tube in the database according to preset data structure and put-in-storage condition of the sample tube. This system specifies the management of biological samples, simplifies operation procedures of put-in-storage and put-out-storage of the biological samples, and accordingly provides conditions for realizing establishment, use and maintenance of a biological sample library.

Description

Biological sample management system based on two-dimensional barcode technology

Technical field

The invention relates to a management system, in particular to a biological sample management system based on two-dimensional barcode technology.

Background technique

The pathogenesis of clinical diseases, early detection and treatment, the designation of individualized treatment programs, the study of molecular mechanisms of anti-tumor metastasis and recurrence, and the development of new anti-tumor drugs are all inseparable from the on-site biological sample bank. Therefore, establishing a sample library of on-site tissue of a certain scale is an inevitable way to further improve the diagnosis and treatment of diseases and improve the long-term efficacy of cancer patients.

Due to the large population, even if biological samples are collected in a specific population, the number of biological samples is still huge, and the number of biological samples is tens of thousands, hundreds of thousands or even millions. For example, biological samples collected include tissue, whole blood, plasma, serum, Biological samples such as DNA, as well as test data generated from specimen testing and analysis procedures and information related to specimens, such as demographic information.

At present, when the biological sample bank is established, the relevant information of the biological sample is mainly manual registration, which is prone to error; and, due to the disordered state of the biological sample storage and delivery, it is difficult and difficult to maintain the biological sample library in the subsequent stage. Locate a biological sample in a large library of biological samples.

Therefore, how to effectively establish an information management system for biological samples for a large number of biological samples, change the disordered state of biological samples into and out of the library, and provide a basis for the rapid positioning of biological samples, has become a technical problem that needs to be solved urgently. one.

technical problem

The invention provides a biological sample management system based on two-dimensional barcode technology In order to solve the current problem of the disordered state of the biological sample storage and delivery, the technical problem is easy to occur, and provide a basis for the rapid positioning of the biological sample.

The invention adopts the following technical solutions: a biological sample management system based on two-dimensional barcode technology, the system comprises: a database; a management server connected to the database; a plurality of management terminals and a barcode printer connected with the management server through the network; Data collector, sample tube;

The sample tube includes a microcentrifuge tube and a sample label;

The microcentrifuge tube comprises a tube body;

The pipe body comprises a lower pipe body at a lower portion and an upper pipe body at an upper portion, the lower pipe body and the upper pipe body being integrally formed integrally;

The sample label is attached to an outer surface of the upper tube;

The sample label is printed with a two-dimensional barcode, the shape of the two-dimensional barcode is square;

A sample label generated by a bar code printer for each microcentrifuge tube storing the biological sample, and a two-dimensional barcode is generated for each microcentrifuge tube storing the biological sample according to the storage position of each sample tube in the refrigerator, the two-dimensional barcode The barcode is printed on the corresponding sample label, and the sample label with the two-dimensional barcode is attached to the microcentrifuge tube;

The sample label and the two-dimensional barcode on the sample tube are read by the data collector, and the biological sample information corresponding to the sample tube is collected;

The management terminal logs in to the management server, and the management server establishes a record data in the database for each sample tube according to the preset data structure according to the preset data structure, and dynamically maintains the database according to the outflow condition of the sample tube.

A further limitation of the above technical solution is that the sample label is an 18-bit two-dimensional barcode, and the first to the first from left to right 18 digits, the first 1-4 digits represent the project number of the biological sample, the 5th digit represents the year of project implementation, and the 9th digit represents the administrative area where the biological sample is investigated, the 10th The number represents the street to be surveyed for each administrative area, the 11th represents the community selected for each street, and the 12th-14th represents each subject selected for the biological sample, 15-16 The bits represent the type of biological sample to be dispensed, and the 17-18th digit represents the serial number of the packed biological sample.

A further limitation of the above technical solution is that the two-dimensional barcode is a 12-bit two-dimensional barcode, and the first to the first from left to right 12 digits, the 1-4th digit represents the refrigerator category in which the sample tube is stored, the 5th to 6th represents the serial number of the refrigerator, the 7th digit represents the tier of the refrigerator, and the 8th to 9th represents the shelf number in the tier, the 10th -12 The bit represents the number of the box in the shelf.

The above technical solution is further defined in that the record data is stored in the 'inbound record data' of the database.

The above technical solution is further defined in that the dynamic maintenance of the database according to the outbound condition of the sample tube means: logging in to the management server through the management terminal, entering the outbound record interface of the database, scanning the sample label of the sample tube of the outbound library, thereby The corresponding record data is found in the 'inbound record data' of the database, and the record data is deleted from the 'inbound record data' of the database to update the database.

The above technical solution is further defined in that the data collector is a mobile data collector, and the management server is connected through a wireless network, or the data collector is a batch data collector connected to the management terminal.

A further limitation of the above technical solution is that the system further includes a barcode scanner connected to the management terminal.

The above technical solution is further defined in that the biological sample information includes at least personal information corresponding to the biological sample, the item name, the sample number, the state at the time of sample collection, the sample size, the sample status, the expiration date, the sample collector, the storage location, The barcode number of the sample label and the information of the storage time.

The above technical solution is further defined in that the lower tubular body is triangular pyramid shaped and hollow inside; the upper tubular body is cylindrical and located at an upper portion of the lower tubular body;

The above technical solution is further defined in that the size of the label sticker is 25 x 8 mm, the outer diameter of the upper tube is 14 mm, and the side length of the two-dimensional barcode is 6-10 mm.

Compared with the prior art, the present invention has the following beneficial effects:

1. The biological sample management system based on the two-dimensional barcode technology proposed by the invention passes the two-dimensional barcode (also known as barcode, barcode The use of the data collector to quickly collect biological samples, changing the traditional tissue biological sample library by manual registration, reducing the possibility of generating errors when building a biological sample library. Moreover, the disorderly and unplanned use of biological samples in storage and delivery is completely avoided.

2 The invention combines the information management technology with the two-dimensional bar code, records the operation, use time and type of the biological sample when the biological sample is put into and out of the library, further standardizes the management of the biological sample library, and simplifies the biological sample library. Operating procedures.

3 The invention increases operational flexibility, and can manage the biological sample library and query the biological sample information on any computer terminal connected to the management server through the network, thereby not only reducing the labor intensity of the staff, but also Rapid positioning to find biological samples provides a basis for rapid identification of target biological samples from millions of biological samples.

4 The present invention can quickly find any biological sample in the biological sample library, and provides conditions for better use of the biological sample library.

1 is a schematic structural view of a system of the present invention;

2 is a schematic diagram of the warehousing process of the biological sample of the present invention;

3 is a schematic diagram of the outflow process of the biological sample of the present invention;

4 is a perspective view of a sample tube of the present invention.

Tens of thousands, hundreds of thousands or even millions of biological samples (such as blood and urine) are stored in sample tubes, each sample is about 100 Microliter to 1 ml, stored in multiple ultra-low temperature refrigerators at -80 °C.

The biological sample management system based on the two-dimensional barcode technology proposed by the invention passes the sample label and the two-dimensional barcode (also called barcode, barcode) The use of biological samples in the storage and delivery of biological samples, recorded the operation, use time and type of biological samples, further standardized the management of the biological sample library, simplifying the operation of the biological sample library.

As shown in FIG. 1 , the biological sample management system based on the two-dimensional barcode technology proposed by the present invention comprises: a database; a management server connected to the database; and a plurality of management terminals and industrial barcode printers and sample tubes connected to the management server through the network.

In a preferred embodiment, a bar code printer, a barcode scanner and a batch data collector are connected to one or each of the management terminals.

In another preferred embodiment, the management server connects one or more mobile data collectors over a wireless network.

In another preferred embodiment, the management server is connected to the management sub-server of the branch structure, and the management sub-server is connected to one or more management terminals, and at least one of the management terminals is connected to the barcode printer, the barcode scanner, and the batch data collection. Device.

Among them, bar code printers and industrial bar code printers are all used to produce and print bar codes according to certain rules.

The biological sample is stored in the sample tube.

The sample tube described above includes a microcentrifuge tube 1 and a sample label 2.

The microcentrifuge tube 1 comprises a tube body 12, a tube cover 14, and a connecting strip 16.

The microcentrifuge tube 1 has a height of 40 mm and a volume of 1.5 to 2.0 ml.

The tubular body 12 includes a lower tubular body 122 at a lower portion and an upper tubular body 124 at an upper portion.

The lower tubular body 122 has a triangular pyramid shape and is hollow inside.

The upper tubular body 124 is cylindrical in shape and is located at an upper portion of the lower tubular body 122. The upper tubular body 124 has an inner diameter of 11 mm and an outer diameter of 14 mm.

The lower tubular body 122 and the upper tubular body 124 are integrally formed as a unitary body.

The lower tubular body 122 and the upper tubular body 124 are made of a sterile inactivated transparent material.

The tube cover 14 includes a cover plate 142 and a closure (not shown).

The cover is vertically protruded from the cover plate 142.

The cap is inserted into the spout of the upper tubular body 124 to cover the tubular body 12.

A space formed between the upper tubular body 124, the lower tubular body 122, and the closure is used to accommodate a biological sample.

The connecting belt 16 is connected between the cover plate 142 of the tube cover 14 and the top end of the upper tube body 122 to prevent the tube cover 14 from falling after being opened, which is difficult to find or contaminated.

The sample label 2 is attached to the outer surface of the upper tube 124. The sample label 2 is printed with information such as the item name, time, address, and code of the biological sample.

The size of the sample label 2 is 25 x 8 mm.

A two-dimensional barcode 22 is printed on the sample label 2.

The two-dimensional barcode 22 has a square shape with a side length of 6-10 mm.

Since the diameter of the upper tubular body 124 is small (the outer diameter is 14 mm), the size of the two-dimensional barcode 22 cannot be made too large, otherwise the scanner can scan the two-dimensional barcode 22 and cannot recognize it. In order to reduce the size, the shape of the two-dimensional barcode 22 is squared and the size is small (the side length is 6-10 mm).

The above two-dimensional barcode 22 represents the storage position of each microcentrifuge tube 1 in an ultra-low temperature refrigerator (-80 ° C), and functions as a positioning.

In one embodiment, the barcode encoding rules for sample tag 2 are designed as follows:

Numbering mode of sample label 2: □□□□ □□□□ □ □ □ □□□ □□ □□

Among them, each "□" represents 1 person, and the first to the 18th are from left to right. Specifically, bits 1-4: the letter A-Z , on behalf of the project number, such as chronic disease flow FMLD; 5-8: digits 0-9, representing the year of project implementation, such as 2008 represents a project implementation year is 2008; 9th: 1 number or 1 Characters, each number or character represents 1 administrative area under investigation; 10th place: 1 number or 1 character, each number or character represents the street to be investigated in each administrative area; 11th place : Number 1-4 , representing the community selected for each street; Figures 12-14: number 001-130, representing the selected subjects in each community; 15-16: the letter AZ, representing the type of sampled sample; 17-18 digits: number 0-9 , representing the serial number of the sample.

In addition, due to the use of multiple ultra-low temperature refrigerators, each refrigerator has its own serial number, such as No. 01 refrigerator; in addition, each refrigerator is divided into: refrigerator > horizon -> shelf -> storage box -> storage location. That is, a plurality of levels are included in a refrigerator, each level includes a plurality of shelves, each shelf including a plurality of storage boxes, each storage box having a plurality of storage locations.

When the staff operates, the operation is generally performed in units of boxes. Therefore, the present invention actually creates a corresponding barcode for each storage box, and the barcode is also generated and printed by a barcode printer and an industrial barcode printer according to a barcode encoding scheme of the storage location. . Specifically, the coding scheme representing the barcode of each storage box or the two-dimensional barcode 22 representing the specific storage location of each sample tube is designed as follows:

Numbering mode of 2D barcode 22: □ □ □ □ □ □ □ □ □ □ □ □ □

Among them, each "□" represents 1 bit, and the 1st to 12th places are from left to right. Position 1-4: Letter A-Z , for cabinet or refrigerator category, such as low temperature refrigerator DWBX; 5-6: digits or letters, representing the serial number of the refrigerator; 7th digit: 0-9, representing the horizon; 8-9: digit 0-9 , represents the shelf number; the 10th-12th digit: the number 001-999, represents the number of the storage box.

Specifically, after the staff accepts the biological sample, the processing flow of the biological sample dispensing and storage is shown in Figure 2:

Step S11: Pre-preserving the biological sample before storage.

Take biological samples for blood and urine as an example. After receiving the biological sample, the staff first check whether the heparin sodium anticoagulant tube, the sodium fluoride anticoagulant tube, and the urine cup are consistent with the quantity indicated on the test report; for the heparin sodium anticoagulant tube, at 4000 rpm Centrifuge at centrifugation for 5-10 minutes, separate plasma and blood cells, see plasma divided into 0.5 ml of 3 sample tubes, store blood cells in 0.5 ml of 2 sample tubes; potassium oxalate sodium fluoride anticoagulant tube at 4000 rpm Centrifugation at a centrifugation speed for 5-10 minutes, separating plasma and blood cells, seeing the plasma divided into 0.5 ml of 2 sample tubes, storing the blood cells into 0.5 ml of 2 sample tubes; dividing the urine in the urine cup into Store 1 ml of 3 sample tubes.

Step S12, generating a unique sample label 2 for each sample tube storing the biological sample.

As described above, after pre-treating the biological sample, after checking the information of the blood vessel, the urine cup and the test report, such as the number and the name, the number of the biological sample is scanned by the barcode scanner to obtain the information of the subject, and the barcode is used. The printer or industrial bar code printer generates and prints 12 sample tags 2 in accordance with the bar code encoding rules on the aforementioned sample tube.

Step S13, generating a unique two-dimensional barcode 22 for each sample tube storing the biological sample.

Determining the storage (refrigeration) position of each sample tube in the refrigerator (ie, which storage box in which the refrigerator is stored in each of the sample tubes), using a bar code printer or an industrial bar code printer according to the bar code encoding rule of the two-dimensional bar code 22 A two-dimensional barcode 22 is generated for each sample tube, printed directly on the corresponding sample label 2, and a sample label 2 on which the two-dimensional barcode 22 has been printed is pasted on each microcentrifuge tube.

Step S14, the staff passes the mobile data collector (such as PDA, Personal Digital Assistant) or batch data collector, collect biological sample information and save it to the database connected to the management server.

Because of the above pretreatment, biological samples of blood and urine are specifically divided into various biological samples such as whole blood, serum, plasma, red blood cells, white blood cells, platelets, blood cells, urine samples, and each biological sample is stored in one sample tube. A sample label 2 with a two-dimensional barcode 22 is provided on each sample tube. Therefore, you only need to use a mobile data collector with a bar code reading function or a batch data collector.

It is possible to create corresponding biological sample information, such as personal information, items, for the biological samples in each sample tube by reading the sample label 2. Name, sample number, state of sample collection, sample size, sample status (hemolysis, normal), expiration date, sampler, storage location, barcode number of sample label 2, storage time, and other biological sample information; A two-dimensional barcode 22 records the storage location of each sample tube in the refrigerator in a database.

The personal information corresponding to the biological sample may include the following information:

A. Demographic information, such as: name, date of birth, gender (male, female), nationality, place of origin, age of in-depth residence, education level (illiterate, elementary school, junior high school, high school/secondary school, college/university/college) , graduate students and above, and add their own types), marital status, occupation (workers, farmers, military personnel, administrative cadres, scientific and technical personnel, medical personnel, teachers, financial and financial services, business service personnel, family women, retirees, unemployed, students Wait, add the type yourself, blood type,

B, smoking: whether smoking now, etc.

C, drinking situation:

D, diet

E, physical activity:

F, health status:

G, mental status:

H, physical examination results: inspector, height, weight, waist circumference, hip circumference, blood pressure (systolic blood pressure, diastolic blood pressure, heart rate).

I, biochemical results: code, data, project name, reference value lower limit, reference value upper limit, unit, prompt

J, fasting blood glucose, total cholesterol, total triglycerides, HDL, LDL, blood uric acid, trace elements (calcium, magnesium, iron, zinc, lead, cadmium, copper, Manganese, aluminum, etc., vitamins (Vit A, Vit B1, Vit B2, Vit B6, Vit B12, Vit C, Vit D1, Vit D2, Vit E, Vit K, etc., antibiotics, etc.

A data structure for recording biological sample information is constructed in advance in the data, and each biological sample information has a corresponding recorded data in the database. In this way, the respective recorded data is created in the database for the biological sample information of each sample tube through the two-dimensional barcode on the sample tube.

In step S15, the worker puts the sample tube into the storage location corresponding to the sample label 2 for storage, and completes the storage operation.

As shown in FIG. 3, the present invention can quickly locate a specific storage location of each sample tube, and specifically includes the following steps:

Step S21: Log in to the database maintained by the management terminal through the management terminal, enter the database, and “inbound record data” in the database. Find the record data corresponding to the sample tube that needs to be out of the library, and find the storage location corresponding to the biological sample.

Since each sample tube has a two-dimensional barcode 22, the two-dimensional barcode 22 corresponds to a unique storage location. Therefore, when searching in the database, various retrieval methods supported by the database, such as input sample tube corresponding to the input, can be used. The "name" sample tube retrieves a plurality of sample tubes corresponding to the name, and parses the two-dimensional barcode 22 of each sample tube to determine which storage box of each refrigerator the sample tube is in.

Step S22: Find the sample tube in the corresponding storage box to perform the outbound operation according to the found storage location.

Step S23, entering the outbound record interface of the database, scanning the sample tag 2 of the sample tube of the library, so that the corresponding record data is found in the “inbound record data” of the database, and the record data is taken from the “inbound record of the database”. The data is deleted to update the database and complete the outflow of the sample tube.

In summary, the present invention has the following beneficial effects as compared with the prior art:

1. The biological sample management system based on the two-dimensional barcode technology proposed by the present invention, through the use of the two-dimensional barcode 22, rapidly collecting biological samples through the data collector, changing the traditional tissue biological sample library by manual registration, reducing the establishment of biological The possibility of errors in the sample library. Moreover, the disorderly and unplanned use of biological samples in storage and delivery is completely avoided.

2. The invention combines the information management technology with the two-dimensional bar code, records the operation, use time and type of the biological sample when the biological sample is put into storage and out of the library, further standardizes the management of the biological sample library, and simplifies the biological sample. The operating procedures of the library.

3. The invention increases operational flexibility, and can manage the biological sample library and query the biological sample information on any computer terminal connected to the management server through the network, thereby not only reducing the labor intensity of the staff, but also Quickly locate the biometric sample to provide a basis for implementation.

4. The present invention can quickly find any biological sample in the biological sample library, and provides conditions for better use of the biological sample library.

The above is only the preferred embodiment of the present invention, and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection of the present invention. Within the scope.

Claims

A biological sample management system based on two-dimensional barcode technology, characterized in that the system comprises: a database; a management server connected to the database; a plurality of management terminals, a barcode printer and a data collector connected to the management server through the network, Sample tube

The sample tube includes a microcentrifuge tube and a sample label;

The microcentrifuge tube comprises a tube body;

The pipe body comprises a lower pipe body at a lower portion and an upper pipe body at an upper portion, the lower pipe body and the upper pipe body being integrally formed integrally;

The sample label is attached to an outer surface of the upper tube;

The sample label is printed with a two-dimensional barcode, the shape of the two-dimensional barcode is square;

A sample label generated by a bar code printer for each microcentrifuge tube storing the biological sample, and a two-dimensional barcode is generated for each microcentrifuge tube storing the biological sample according to the storage position of each sample tube in the refrigerator, the two-dimensional barcode The barcode is printed on the corresponding sample label, and the sample label with the two-dimensional barcode is attached to the microcentrifuge tube;

The sample label and the two-dimensional barcode on the sample tube are read by the data collector, and the biological sample information corresponding to the sample tube is collected;

The management terminal logs in to the management server, and the management server establishes a record data in the database for each sample tube according to the preset data structure according to the preset data structure, and dynamically maintains the database according to the outflow condition of the sample tube.
The biological sample management system based on the two-dimensional barcode technology according to claim 1, wherein the sample label is an 18-bit two-dimensional barcode, which is respectively from left to right. From 1st to 18th, the first 1-4th represents the project number of the biological sample, the 5th digit represents the year of project implementation, and the 9th represents the administrative area where the biological sample is investigated, the 10th The number represents the street to be surveyed for each administrative area, the 11th represents the community selected for each street, and the 12th-14th represents each subject selected for the biological sample, 15-16 The bits represent the type of biological sample to be dispensed, and the 17-18th digit represents the serial number of the packed biological sample.
The biological sample management system based on the two-dimensional barcode technology according to claim 1, wherein the two-dimensional barcode is a 12-bit two-dimensional barcode, from left to right respectively. 1st to 12th, the 1-4th represents the refrigerator category in which the sample tube is stored, the 5th to 6th represents the serial number of the refrigerator, the 7th represents the tier of the refrigerator, and the 8th to 9th represents the shelf in the tier Number, number 10-12 digits represent the number of the storage box in the shelf.
According to claim 1 The biological sample management system based on the two-dimensional barcode technology is characterized in that the recorded data is stored in the 'inbound record data' of the database.
According to claim 4 The biological sample management system based on the two-dimensional barcode technology is characterized in that the dynamic maintenance database according to the outflow condition of the sample tube means: logging in to the management server through the management terminal, entering the outbound record interface of the database, and scanning out the database. The sample tag of the sample tube, so that the corresponding record data is found in the 'inbound record data' of the database, and the record data is deleted from the 'inbound record data' of the database to update the database.
According to claim 1 The biological sample management system based on the two-dimensional barcode technology is characterized in that the data collector is a mobile data collector, and the management server is connected through a wireless network, or the data collector is a batch processing method for connecting the management terminal. Data collector.
According to claim 1 The biological sample management system based on the two-dimensional barcode technology is characterized in that the system further comprises a barcode scanner connected to the management terminal.
According to claim 1 The biological sample management system based on the two-dimensional barcode technology is characterized in that the biological sample information includes at least personal information corresponding to the biological sample, a project name, a sample number, a state at the time of sample collection, a sample size, a sample state, and an effective Information on the term, the sampler, the storage location, the barcode number of the sample label, and the time of the deposit.
A biological sample management system based on a two-dimensional barcode technique according to claim 1, wherein The lower tubular body is triangular pyramid shaped and hollow inside; the upper tubular body is cylindrical in shape and is located at an upper portion of the lower tubular body.
A biological sample management system based on a two-dimensional barcode technique according to claim 1, wherein The label sticker has a size of 25 x 8 mm, the upper tube has an outer diameter of 14 mm, and the two-dimensional barcode has a side length of 6-10 mm.