WO2013171004A1 - Device for introducing samples into a micro-fluidic system - Google Patents

Device for introducing samples into a micro-fluidic system Download PDF

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Publication number
WO2013171004A1
WO2013171004A1 PCT/EP2013/057315 EP2013057315W WO2013171004A1 WO 2013171004 A1 WO2013171004 A1 WO 2013171004A1 EP 2013057315 W EP2013057315 W EP 2013057315W WO 2013171004 A1 WO2013171004 A1 WO 2013171004A1
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WO
WIPO (PCT)
Prior art keywords
channel
component
adapter component
collar
fluid
Prior art date
Application number
PCT/EP2013/057315
Other languages
German (de)
French (fr)
Inventor
Thomas BRETTSCHNEIDER
Christian Dorrer
Original Assignee
Robert Bosch Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE102012208074.0 priority Critical
Priority to DE102012208074A priority patent/DE102012208074A1/en
Application filed by Robert Bosch Gmbh filed Critical Robert Bosch Gmbh
Publication of WO2013171004A1 publication Critical patent/WO2013171004A1/en

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02Adapting objects or devices to another
    • B01L2200/026Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0672Integrated piercing tool
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0681Filter
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56Labware specially adapted for transferring fluids
    • B01L3/563Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors

Abstract

The invention relates to a device for introducing samples into a microfluidic system, comprising an adapter component that has an upper surface and lateral surfaces arranged perpendicularly thereto, said adapter component having a first and a second hollow needle which extend from the upper surface of the adapter component, said first and second hollow needle each being in fluid connection with a channel that runs, within the adapter component, to a first or a second lateral surface of said adapter component, the hollow needles which extend from the upper surface being surrounded together by a collar that extends from the upper surface so as to be higher than the hollow needles, said adapter component being able to be coupled to the microfluidic system via the first and the second channel, and the collar being designed to receive a sample removal device wherein the sample to be removed can be conducted away from said sample removal device and into one or both channels by means of the first and/or the second hollow needle.

Description

 Description title

 Device for sample introduction into a microfluidic system

The present invention relates to a device for sample introduction into a

Microfluidic system, in particular in a lab-on-a-chip (LOC) system.

State of the art

 The prior art discloses LOC systems, which are often referred to as μΤΑ5 (micro-total-analysis-systems), which generally serve to assemble samples, e.g. To analyze samples of patients, such as blood, urine, sputum, or other body fluids completely and automatically, the small size of these systems is very significant, so they are particularly used for mobile applications where a full analysis must be carried out quickly, e.g. at the medical first aid. In the practice of these systems, the introduction of the sample into these systems can be critical, for example, in preventing contamination of the sample, which is typically manual, e.g. by means of a pipette, from a standardized sampling device in an opening of a LOCs is made. From DE 60 2005 001 235 T2, for example, such a microfluidic system is known in which a sample or fluid supply mechanism of the

Sample entry is controlled by a user, with this mechanism, after the introduction of the sample into an opening on the LOC, valves on the LOC opened or

must be closed in order to introduce a sample into the LOC.

Disclosure of the invention

The invention proposes in a first aspect according to claim 1, a device for sample introduction into a microfluidic system, wherein the device a

Adapter component having an upper surface and arranged substantially perpendicular to the upper surface disposed side surfaces, wherein the adapter component a first hollow needle and a second hollow needle, which are each in a

Spacing vertically apart from the upper surface of the adapter component, wherein the first hollow needle in fluid communication with a running within the adapter component to a first side surface of the adapter component first channel, and separately from the second hollow needle with a within the

Adapter component is in fluid communication with a second side surface of the adapter component extending second channel, wherein the perpendicularly extending from the upper surface of the hollow needles are jointly surrounded by a collar which extends higher than the hollow needles of the upper surface, and wherein the

Adapter component can be coupled via the first and second channel to the microfluidic system, and the collar is adapted to receive a sampling device, wherein the sample to be sampled on the first and / or second

Hollow needle from the sampling device and in one or both channels can be discharged.

Advantages of the invention

The advantage of the proposed device arises from the fact that a sample is continuous, i. from removal from the patient to completion of the analysis, remaining in sealed containers so that contamination of the sample by contact with the environment and / or the user is excluded. Also, contamination of the environment by the sample is excluded. The user inserts the sampling system onto the LOC just at the beginning of the analysis, eliminating the need for manual pipetting. The transport of the sample from the sampling system into the LOC is automated. Thus, advantageously, there is additional time savings and easier operation, thereby reducing operator error, e.g. the pipetting of wrong sample quantities or the confusion of samples from different patients should be excluded. Advantageously, the first channel and / or the second channel with a

Fluid reservoir within the adapter component with relative to the respective volumes of the channels increased volume in fluid communication. This has the advantage that the sample can be further processed directly in the reservoir, for example by mixing with reagents located upstream in the reservoir, for example with reagents for digesting the biological sample. It is preferred that the adapter component comprise an upper component and a lower component, wherein the hollow components, the collar and the channels are integrated into the upper component, namely the channels in the manner of recesses in the upper component, and the lower component and the upper component are connected together in the manner of a sandwich. This has the advantage that a simple and inexpensive production is possible.

It is further preferred that a film-like layer is disposed between the upper component and the lower component, and the lower component has channel-like recesses for the first and second channels which are fluidly separated from the channels in the upper component by the film-like layer. This has the advantage that the film-like layer can be used for joining the layer structure, the film-like layer mechanical stresses in the

Layer structure can compensate, and in the film-like layer also active elements, such as. Valves, with integrable.

It is also preferred that the collar is claw-shaped to releasably secure the sampling device in the collar. This has the advantage that an even safer fit of the removal device on the

Adapter component or the LOC is achieved and a faulty operation is prevented because a defined insertion depth of the removal device can be specified in the collar.

Preferably, when the adapter component is not used above the collar, a layer which seals the cavity needles but is detachable or pierceable is disposed. This has the advantage that contamination of the needles and channels during non-use or storage is avoided.

Advantageously, the first channel is fluidically connected to a reservoir, which contains a fluid, for example air, wherein the reservoir can be acted upon by a mechanical, hydraulic or pneumatic pressure, that the first channel by means of the fluid in the reservoir with a fluid pressure can be acted upon. In this case, the reservoir can be configured such that the first channel is enlarged in a recess-like manner at least along a section of the first channel, and the fluid pressure in the first channel can be generated by means of an actuation channel which extends substantially along the recess-like enlargement the first channel is arranged and is fluidically separated from the first channel by an elastically or plastically deformable membrane, and a fluid in the Aktuierungskanal is pressurized, so that the membrane in the direction of

displaced recess-like enlargement of the first channel, and thus the fluid pressure in the first channel can be generated. This has the advantage that the first channel, the supply channel is not connected to the outside world, and thus contamination is prevented.

In addition, it is preferred that the reservoir comprises a housing which is fluidically connected to the first channel, and the housing a displaceable

Housing wall section, e.g. by a membrane in the housing wall portion, which can be acted upon by a mechanical, hydraulic or pneumatic pressure, so that the displaceable housing wall portion in the direction

Housing interior is displaced, in which there is a fluid, so that the first channel is acted upon by a fluid pressure. This has the advantage that the first channel, the supply channel is not connected to the outside world, and thus contamination is prevented. In addition, the housing with the reservoir located therein can be made relatively large. Then it is further preferred that the displaceable housing wall section is displaceable in the direction of the housing interior by means of a mechanical pressure, wherein the displaceable housing wall section can also be displaced in the manner of a stamp by means of the mechanical pressure in the direction of the housing interior. This has the advantage that no hydraulic or pneumatic pressure connection must be provided and thus the operation and handling of the microfluidic system is simplified.

BRIEF DESCRIPTION OF THE DRAWINGS In the following, the invention is explained by means of embodiments in conjunction with the figures, in which:

Figure 1 is a schematic cross-sectional side view of a first embodiment of the invention;

Figure 2 is a plan view of the device of Figure 1; Figure 3 is a schematic cross-sectional side view of the device of Figure 1, together with a sampling device;

Figure 4 is a schematic cross-sectional side view of a second embodiment of the invention;

Figure 5 is a schematic cross-sectional side view of a third embodiment of the invention;

Figure 6 is a schematic cross-sectional side view of a fourth embodiment of the invention;

Figure 7 shows a schematic cross-sectional side view of a fifth embodiment of the invention;

Figure 8 is a schematic cross-sectional side view of a sixth embodiment of the invention;

Figure 9 is a schematic cross-sectional side view of a seventh embodiment of the invention;

Figure 10 is a schematic cross-sectional side view of an eighth embodiment of the invention;

Figure 1 1 shows a schematic cross-sectional side view of a ninth embodiment of the invention; and

Figure 12 shows a schematic cross-sectional side view of a tenth embodiment of the invention.

Embodiments of the invention

With reference to Figure 1, a first embodiment of the invention will now be explained. FIG. 1 shows, in a schematic side cross-sectional view, an adapter component 10 according to a first embodiment of the invention, wherein the

Adapter component 10 (also referred to as a fluidic adapter) is part of a LOC for clarity not shown here.

As can be seen in FIG. 1, the adapter component 10 comprises a housing body 20 with a first side surface 30 (hereinafter also referred to as first end surface 30) and a second side surface 40 (hereinafter also referred to as second end surface 40), as well as an upper surface 50, wherein the side surfaces 30, 40 in

Are arranged substantially perpendicular to the upper surface 50. By doing

 Housing body 20 extend a first channel 60 and a second channel 70, which respectively open into the first end face 30 and the second end face 40. Approximately toward the center of the housing body 20, the channels 60, 70 are substantially perpendicular relative to the upper surface 50, and are formed outside the housing body 20 as hollow needles 80, 90. The channels 60, 70 form a channel system of at least two channels (as shown), the first channel 60, as will be explained in more detail below, serving as a feed channel and the second channel 70 as a bleed channel. A height h3 of the channels 60, 70 is in the range of 0.01 to 5.0 mm, and is preferably 0.3 mm.

As is known in the art, the channel system serves to transport sample material to reaction and analysis chambers in a LOC to which the adapter component 10 is coupled (not shown). The hollow needles 80, 90 are surrounded by a collar 100, which extends from the upper surface 50 via the hollow needles 80, 90. A height h1 of the collar is in the range of 1 to 40 mm, and is preferably 20 mm. A length h2 of the hollow needles 80, 90 is in the range of 1 to 40 mm, and is preferably 10 mm. Thus, the

Hollow needles 80, 90 protected against accidental contact by the user with appropriate choice of h1 and h2.

The collar 100 may be integrally formed with the housing body 20, but it is also conceivable that the collar 100 is mounted as a component on the upper surface 50 of the housing body 20. As can be seen in Figure 2, which is a plan view of the adapter component 10 and the housing body 20 with its upper surface 50, the collar 100 is arranged in a circle around the hollow needles 80, 90, wherein an inner diameter d1 of the collar 100 in the range of 5 to 30 mm, and preferably 8 mm, and a

Outer diameter d2 of the collar 100 is in the range of 6 to 50 mm, and is preferably 10 mm.

Furthermore, in Figure 2, indicated by dashed lines, the channels 60, 70 can be seen, wherein a width w1 of the channels 60, 70 is in the range of 0.01 to 5.0 mm, and preferably 0.3 mm, and thus essentially corresponds to the channel height h3 (see Figure 1). In addition, it can be seen in FIG. 2 that the channels 60, 70 each open into the end faces 30 and 40, respectively, of the housing body 20, specifically for coupling to a LOC (not shown here). The functional principle of the adapter component 10 will now be explained with reference to FIG.

FIG. 3 shows the adapter component 10 in the schematic side cross-sectional view as in FIG. 1. In addition, FIG. 3 shows a sampling device 110, which essentially consists of a sample tube 120 with a sample to be examined, wherein the sample tube 120 is inserted into an end 130 of a tubular screw cap 135. The other end 140 of the tubular screw cap 135 is sealed by a septum 150 against outflow and contamination of the sample contained in the tube 120.

As shown in Figure 3, the end 140 of the screw cap 135 and the removal device 1 10 is located within the collar 100, with the membrane 150 just above the pointed hollow needles 80, 90. If the

Removal device 1 10 further moves in the direction of the hollow needles 80, 90, they pierce the membrane 150, whereby fluidic connections between the channels 60, 70 and the interior of the tube 120 are produced. To empty the tube 120, an overpressure is applied to the channel 60, the feed channel, e.g. one

pneumatic overpressure, so that, via the hollow needle 90, an overpressure in the tube 120 is generated, which brings the sample located in the tube 120 to drain via the hollow needle 80 and the channel 70, the discharge channel. From the channel 70, the sample enters a coupled LOC or in its reaction analysis chambers. By Flushing a liquid, such as a buffer, through channel 60, the feed channel, also allows solid samples to be flushed out of tube 120.

The overpressure in the feed channel 60 may be generated by a pump located outside the adapter component 10, e.g. in a coupled LOC or as an external component, e.g. as a syringe or peristaltic pump, and can be controlled via valves located outside and / or inside the adapter component 10 and / or the LOC (not shown in FIG. 3). The overpressure is in a range of 10 to 2000 mbar, and is preferably between 50 and 200 mbar.

In addition to the channel system and at least one pump, the adapter component 10 or the LOC may contain further fluidic or microfluidic elements, e.g.

Valves, chambers, mixers, filters, strainers and / or other channels. The inner diameter d1 of the collar is matched to the shape of the removal device 110 or the screw cap 135 in such a way that a secure fit of the

Removal device 1 10 results on the adapter component 10. It is particularly advantageous that the material of the collar 100, e.g. a polymer such as polyethylene, has a certain flexibility, or the thickness of the collar (i.e., d2-d1) is designed so that when attaching the removal device 1 10 a slight elastic deformation of the collar 100 occurs, whereby an even better fit of the

Removal device is achieved on the adapter component 10.

FIG. 4 shows a further embodiment of an adapter component 10 in a schematic side cross-sectional view.

It should be noted at this point that, with respect to the figures and the description, like reference numerals refer to the same or similar elements. The adapter component 10 shown in FIG. 4 essentially corresponds to the adapter component illustrated in FIG. 1, but with the difference that the second channel 70, the discharge channel, opens into a fluid reservoir 160. This has the advantage that the sample can be further processed directly in the reservoir 160, for example by mixing with reagents upstream in the reservoir. Otherwise, the elements shown in Figure 4, ie the channel 60, the cavity needles 80, 90 and the collar 100 substantially correspond to the elements described with reference to Figure 1. FIG. 5 shows a further embodiment of an adapter component 10 in a schematic side cross-sectional view. Here, the adapter component 10 consists of a multi-layer structure, ie, in the example shown, an upper component 170 and a lower component 180. The upper component 170 is on its underside 171 with so

Recesses structure that channels 60 and 70 are formed when the upper component 170 is connected to the lower component 180, e.g. by gluing or welding. The upper surface 181 of the lower component 180 is not patterned in the illustrated example, i. just.

The components 170, 180 may be made of polymers, e.g. thermoplastic polymers, for example PC (polycarbonate), COC (cyclo-olefin copolymer), COP (cyclo-olefin polymer), PE (polyethylene) or PP (polypropylene). The underside 171 of the component 170 is structured, for example, by milling, hot stamping or injection molding.

The hollow needles 80, 90 may be made of metal, e.g. Steel, or also made of a polymer, and are glued or pressed into the component 170 or made integral therewith, e.g. by molding.

The thickness h4 of the upper component 170 and the thickness h5 of the lower component 180 are each in the range of 0.1 to 30 mm, and is preferably 1 mm.

It is also conceivable that the sandwich construction of the adapter component has more than two or three components, which depends, for example, on the type of application. FIG. 6 shows a further embodiment of an adapter component 10 in a schematic side cross-sectional view.

In this case, in contrast to the embodiment illustrated in FIG. 5, between the upper component 170 and the lower component 180 there is another component 190, which is a foil which may be made of an elastomer, a thermoplastic or a thermoplastic elastomer, for example. in fact with a thickness in the range of 0.01 to 1 mm, preferably 0.1 mm. The arrangement of a film 190 between the upper and lower components 170, 180 has the advantage that an even easier production of the adapter component 10 is possible because the film 190 can compensate for mechanical stresses in the layer structure, and with the film 190 active elements, such as Valves, can be integrated within the adapter component 10, and the film can be used to join the components.

In the embodiment shown in Figure 6, the top 181 of the lower

Component 180 is structured with recesses analogous to the example shown in Figure 5 with respect to the bottom 171 of the upper component 170, that in a composite of the components 170, 180, 190, the channels 60, 70 are formed. In this case, there are openings 61 or 71 in the film 190 in order to permit a fluidic connection to the cavity needles 80, 90 in each case.

It should be noted that both the upper surface 181 of the lower component 180 and the lower surface 171 of the upper component 170 may be patterned, as will be shown below by way of example. FIG. 7 shows a further embodiment of an adapter component 10 in a schematic side cross-sectional view.

The adapter component 10 shown in FIG. 7 corresponds to the example shown in FIG. 6 with regard to its construction with three components 170, 180, 190, but has a collar 100 of different design. This has at its upper end 101 a claw-like structure 102 with claws 104, which holds the removal device 1 10 particularly safe when it is inserted into the collar 100. Since the material of the collar 100, as mentioned above, is elastic, presses the removal device 1 10 with its screw cap 135, which is slightly larger in outer diameter than that

Inner diameter of the claw-like structure 102, when inserted into the collar 100, the structure 102 to the side, which is facilitated by beveled surfaces 103 on the claw 104 until the screw cap 135 with its shoulder 136 below the jaws 104 "snaps" so that a Particularly secure fixation is achieved because the claw-like structure 102 rests against the outer surface of the screw cap 135 (here, the distance between the screw cap 135 and the inside of the claw-like structure 102 is exaggerated in Figure 7 for clarity). In this way is also a particularly good leadership for the removal device 1 10 scored.

In addition, a misuse is prevented, since by a formed in the collar 100 paragraph 105 at a predetermined distance from the upper surface 50 of

Housing body 20 is given a defined insertion depth for the removal device 1 10, when this abuts with their sales area 137 on the paragraph 105.

Although not shown, the claw-like structure 102 is divided into a plurality of segments along its circumference, so that the respective segments can yield to the mechanical pressure by the removal device 110 upon insertion into the collar 100.

FIG. 8 shows a further embodiment of an adapter component 10 in a schematic side cross-sectional view. Here, the only difference with respect to the previous embodiments is that the upper end 101 of the collar 100 is sealed with a foil 200 in a releasable or puncturable manner when the adapter component 10 is not in use. Thus, contamination of the space formed by the collar 100 space 210 or the cavity needles 80, 90 and the channels 60, 70 is avoided.

The film 200 may be made of, for example, aluminum, a thermoplastic polymer, a coated thermoplastic polymer, e.g. one with a metal, e.g.

Aluminum, coated thermoplastic polymer, rubber or other suitable material, and may be bonded to the upper end 101 of the collar 100 by gluing or welding or mechanical stress, although the foil 200 may not be shown explicitly in FIG. can extend over the edge of the collar 100.

FIG. 9 shows a further embodiment of an adapter component 10 in a schematic side cross-sectional view.

The embodiment shown in FIG. 9 essentially corresponds to the embodiment shown in FIG. 6, but with the following difference: From the first end face 30 of the housing body 20, an actuation channel 220 extends along a

Section of the first channel 60, in the form of a recess at the bottom 171 of the first component 170. This actuation channel 220, the function of which will be explained below, is fluidically separated from the first channel 60 by the layer 190.

The first channel 60, which is structured on the upper side 181 of the second component 180, but does not open in the first end face 30, has a

recess-like enlargement 230, which at least partially covers the Aktuierungskanal 220.

The Aktuierungskanal 220 now serves to be acted upon with a pneumatic pressure from outside the end face 30, so that the layer 190, which is at least along the Aktuierungskanals 220 in the manner of a membrane elastically or plastically deformable, displaced into the recess 230 inside is, whereby the pressure in the first channel 60 is transmitted, and thus a used in the collar 100 unloading device (not shown here) is emptied into the second channel 70, see also the description with reference to Figure 3.

This has the advantage that the first channel 60, the feed channel, which does not open into the first end face 30, is not connected to the outside world, and thereby a

Contamination of the channel system from the environment, e.g. through contaminated

Compressed air is prevented.

FIG. 10 shows a further embodiment of an adapter component 10 in a schematic side cross-sectional view. The embodiment illustrated in FIG. 10 essentially corresponds to the embodiment shown in FIG. 5, but with the following difference: The first channel 60, in turn, not in the first end face 30, but in a housing 250 forming a reservoir 240, analogously to the previous example , which is arranged as a separate or integrated component on the upper surface 50 of the first component 170. Via an opening 260 in the housing 250, the first channel 60 is fluidically connected to the reservoir 240.

In a side wall or housing wall portion 251 of the housing 250 is for

Housing inside a membrane 252 arranged, which is acted upon via an actuation channel 220 in the side wall 251 with a pneumatic pressure, so that the diaphragm 252 displaced into the reservoir 240, whereby the pressure in the first channel 60 is transmitted, and thus a used in the collar 100

Removal device (not shown here) is emptied into the second channel 70, see again the description with reference to Figure 3. This in turn has the advantage that the first channel 60, the feed channel, which does not open into the first end face 30, is not connected to the outside world, and thereby a contamination of the channel system from the environment, eg through contaminated

Compressed air is prevented. Similarly, those shown in Figs. 11 and 12 function

 Embodiments that differ only slightly in construction from each other, and therefore will be described together below.

The embodiments shown in FIGS. 11 and 12 essentially correspond to the embodiment shown in FIG. 10, but with the following differences: In the embodiments shown in FIGS. 11 and 12, a pressure is not transmitted pneumatically to a diaphragm, but a pressure becomes mechanical , indicated in each case by an arrow 260, exerted on a stamp 270, which acts on the side wall 251, and is thus transferred to the reservoir 240. The deformation of the housing 250 may be elastic or plastic.

In FIG. 12, in contrast to the embodiment in FIG. 11, the side wall 251 can be displaced into the reservoir 240 in the manner of a piston relative to the housing. The advantage of the previously described embodiments with reference to Figures 10 to 12 is firstly that the reservoir 240, i. the displacement chamber can be relatively larger than the "displacement chamber" 230 shown in FIG. 9, so that even larger volumes can be displaced out of the sampling device; for another, the advantage lies in the fact that, with reference to FIGS. 11 and 12, no

pneumatic connection must be provided and thus the operation and handling of the microfluidic system is simplified.

Finally, it should be noted that individual characteristics of the different

Embodiments can also be combined with each other, even if this is not explicitly shown here, and if it makes sense technically. Incidentally, the serve dimensions shown are illustrative only, and are not drawn to scale.

Claims

claims
1 . Device for introducing samples into a microfluidic system, comprising an adapter component (10) with an upper surface (50) and side surfaces (30, 40) arranged substantially perpendicular to the upper surface (50), wherein the adapter component (10) comprises a first hollow needle ( 80) and a second hollow needle (90) each extending at a distance from each other perpendicularly from the upper surface (50), wherein the first hollow needle (80) with a within the adapter component (10) to a first side surface (30). of the
 Adapter component (10) extending first channel (60) is in fluid communication, and separately from the second hollow needle (90) with a within the
 Adapter component (10) to a second side surface (40) of
 Adapter component (10) extending second passage (70) is in fluid communication, wherein the perpendicular from the upper surface (50) extending
 Hollow needles (80, 90) are jointly surrounded by a collar (100) which extends higher than the hollow needles (80, 90) from the upper surface (50), and wherein the adapter component (10) via the first channel (60). and the second channel (70) can be coupled to the microfluidic system, and the collar (100) for
 Receiving a sampling device (1 10) is formed, wherein the sample to be sampled via the first and / or second hollow needle (80, 90) from the sampling device (1 10) and in the second channel (70) can be discharged.
The apparatus of claim 1, wherein the first channel (60) and / or the second channel (70) includes a fluid reservoir (160) within the adapter component (10) having increased volume relative to the respective volumes of the channels (60, 70) in fluid communication.
3. Device according to claim 1 or 2, wherein the adapter component (10).
 at least one upper component (170) and at least one lower component (180), wherein in the upper component (170) the hollow needles (80, 90) and the collar (100) are integrated, and wherein the channels (60, 70) in the upper one
Component (170) or the lower component (180) are integrated, namely the channels (60, 70) in the manner of recesses in the upper component (170) or the lower component (180), and wherein the lower component (170 ) and the upper component (180) are connected together in the manner of a sandwich. The device of claim 3, wherein a film-like layer (190) is disposed between the upper component (170) and the lower component (180).
Apparatus according to any one of the preceding claims, wherein the collar (100) is claw-shaped to releasably secure the sampling means (110) in the collar (100).
Device according to one of the preceding claims, wherein when non-use of the adapter component (10) above the collar (100) a cavity the needles (80, 90) sealing, but releasable layer (200) is arranged.
Device according to one of claims 1 to 6, wherein the first channel (60) in fluid communication with a reservoir (240), which includes a fluid, wherein the reservoir (240) is acted upon by a mechanical pressure (260) the first channel (60) can be acted on by means of the fluid in the reservoir (240) with a fluid pressure.
The apparatus of claim 7, wherein the reservoir (240) is configured such that the first channel (60) is recessed (230) at least along a portion of the first channel (60), and wherein the fluid pressure in the first channel (60) is increased. by means of an actuation channel (220) can be generated, which in
Substantially disposed along the recess-like enlargement (230) of the first channel (60) and is fluidly separated from the first channel (60) by a membrane (190), and a fluid in the Aktuierungskanal (220) is pressurized, so that the membrane (190) in the direction of
recess-like enlargement (230) of the first channel (60) displaces, and thus the fluid pressure in the first channel (60) is transferable.
The apparatus of claim 7, wherein the reservoir (240) includes a housing (250) fluidly communicating with the first channel (60), and wherein the housing (250) includes a displaceable housing wall portion (251) which is pressurized is acted upon, so that the relocatable
Housing wall portion (251) in the direction of Gehäuseinnerem is displaced, in which a fluid is, so that the first channel (60) can be acted upon by a fluid pressure.
10. Apparatus according to claim 9, wherein the displaceable Gehausewandabschnitt (251) comprises a membrane (252), which by means of a fluid in the direction of
 Housing interior is displaced.
1 1. Apparatus according to claim 9, wherein the displaceable Gehausewandabschnitt (251) by means of a mechanical pressure (260) is displaceable in the direction of the housing interior. 12. The apparatus of claim 1 1, wherein the displaceable housing wall portion (251) in the manner of a punch (270) by means of the mechanical pressure (260) in the direction of the housing interior is displaceable.
PCT/EP2013/057315 2012-05-15 2013-04-08 Device for introducing samples into a micro-fluidic system WO2013171004A1 (en)

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