WO2013133753A1 - Plants having improved growth properties - Google Patents
Plants having improved growth properties Download PDFInfo
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- WO2013133753A1 WO2013133753A1 PCT/SE2013/050191 SE2013050191W WO2013133753A1 WO 2013133753 A1 WO2013133753 A1 WO 2013133753A1 SE 2013050191 W SE2013050191 W SE 2013050191W WO 2013133753 A1 WO2013133753 A1 WO 2013133753A1
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- plant
- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Definitions
- the invention relates to a method for producing a genetically modified plant with improved growth properties as compared to a corresponding non-genetically modified wild type plant, said method comprising reducing or deleting the amount or activity of an EBI1 or EBI2 polypeptide in a plant cell, a plant or a part thereof.
- Plants use light-dark cues and an internal 24-h (circadian) clock to orient themselves in their local environment and to synchronize their metabolism accordingly.
- the circadian clock of the model plant Arabidopsis (Arabidopsis thaliana) is made up of a complex series of interacting feedback loops whereby proteins regulate their own expression across day and night.
- Early bird (ebi) is a circadian mutation that causes the clock to speed up: ebi plants have short circadian periods, early phase of clock gene expression, and are early flowering.
- the gene responsible for the ebi-1 phenotype, AtNFXL-2 is a zinc finger transcription factor, a homolog of the human NF-Xl protein.
- NF-Xl binds to the X-box found in class II MHC genes.
- Arabidopsis has two NF-Xl homologs, AtNFXL-1 and AtNFXL-2, which are thought to act antagonistically to regulate genes involved in salt, osmotic and drought stress, with AtNFXL-1 activating and AtNFXL-2 repressing stress-inducing genes.
- AtNFXL-1 has also been suggested to be a negative regulator of defense-related genes and temperature stress.
- the clock phenotype of the AtNFXL-2 mutant provides an interesting link between the clock and biotic and abiotic stress responses. This link has been alluded to in a recent review and in the identification of a possible role for the clock protein GI in cold stress tolerance.
- the circadian phenotypes of the ebi-1 mutant have been characterized by Johansson, M. et al. (2011) Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock. Plant Physiol. 155(4): 2108-2122.
- EBI1 genes EBIla (SEQ ID NO: 1) and EBIlb (SEQ ID NO: 3) as well as two EBI2 genes: EBI2a (SEQ ID NO: 6) and EBI2b (SEQ ID NO: 8). See also Johansson et al. (2011), Supplemental Table I and Supplemental Figure 1.
- Figure 1 A illustrates diurnal expression of Populus EBI1 from Real time PCR Biological repeat 1.
- the Y-axis is representing the relative expression (PttEBIla/Pttl8S)
- Figure IB illustrates the light induced diurnal expression of Populus EBI2 from Real time PCR Biological repeat 1.
- the Y-axis is representing the relative expression (PttEBI2a/Pttl8S).
- Figure 1C illustrates the light induced diurnal expression of Populus EBI1 from Real time PCR Biological repeat 2.
- the Y-axis is representing the relative expression (PttEBIla/Pttl 8S)
- Figure ID illustrates the light induced expression of Populus EBI2 from Real time PCR Biological repeat 2.
- the Y-axis is representing the relative expression (PttEBI2a/Pttl8S).
- Figure IE illustrates the diurnal expression of Populus EBIl .
- the two Y-axises are representing the expression level of EBIla and EBIlb, respectively.
- LDHH data were obtained from the diurnal data base [http: //diurnal. cgrb. oregonstate. edu/] *
- Figure IF illustrates the diurnal expression of Populus EBI2a.
- the two Y-axises are representing the expression level of EBI2a and EBI2b, respectively.
- the LDHH data is from the diurnal data base.
- Figure 1 G illustrates expression of EBIl in various Populus tissues (data from Poplar eFP Browser at http://bar.utoronto.ca/).
- Figure 1 H illustrates expression of EBI2 in various Populus tissues (Poplar eFP Browser).
- the tissues are mature leaf (M); young leaf (L); root (R); dark-grown seedling etiolated (S); dark -grown seedling, etiolated, exposed to light for 3 hours (S3); continuous light- grown seedling (CS); female catkins (FC); male catkins (MC) and xylem (X).
- the Y-axis is representing the expression level.
- Figure 2 shows elongation and radial growth in transgenic Populus trees wherein EBIl (Fig. 2A) and EBI2 (Fig. 2B), have been knocked out down by RNA interference.
- T89 indicates a wild type tree.
- the left Y-axis is representing the height in cm.
- the right Y-axis is representing the diameter in mm.
- the X-axis is representing the time in days.
- Figure 3 illustrates the ratio (mutant/WT) of Populus EBIl (Fig. 3 A) and EBI2 (Fig. 3B) expression in transgenic trees wherein EBIl and EBI2, respectively, have been knocked out down by RNA interference.
- the X-axis is representing the time in days under short days.
- EBI1 EARLY BIRD1
- EBI2 EARLY BIRD2
- the growth phenotype is inversely proportional to the level of expressed transcript (more growth when there is less transcript) indicating that the effect is due to the down-regulation of the targeted transcripts. It is suggested that EBI genes are useful as targets for down-regulation to obtain increased growth and generating more biomass of forest trees.
- the invention provides a method for producing a genetically modified plant with improved growth properties as compared to a corresponding non-genetically modified wild type plant, said method comprising:
- improved growth properties should be understood as primary growth, including a lengthening of the stem and roots, as well as secondary growth of a plant, including production of secondary tissue, "wood", from the cambium and an increase in the girth of stems and roots.
- One way of following the growth might be by measuring the height and the diameter of the stem and optionally calculating the volume of the stem and compare it with a wild type population or with parental control trees of the plant of interest.
- the method according to the invention comprises the additional steps of: (c) selfing or crossing the genetically modified plant with itself or another plant, respectively, to produce seed; and
- the said EBIl polypeptide comprises a domain having at least about 161 amino acids, said domain being at least 75% identical, such as 80%, 85%, 90%, 95% or 100% identical, with the amino acid sequence shown as SEQ ID NO: 5. More preferably, the said EBIl polypeptide has an amino acid sequence which is at least 75%, such as 80%, 85%, 90%, 95% or 100%, identical with the sequence shown as SEQ ID NO: 2 (EBIl a) or SEQ ID NO: 4 (EBIlb).
- the said EBI2 polypeptide comprises a domain having at least about 191 amino acids, said domain being at least 75% identical, such as 80%, 85%, 90%, 95% or 100% identical, with the amino acid sequence shown as SEQ ID NO: 10. More preferably, the said EBI2 polypeptide has an amino acid sequence which is at least 75%, such as 80%, 85%, 90%, 95% or 100%, identical with the sequence shown as SEQ ID NO: 7 (EBI2a) or SEQ ID NO: 9 (EBI2b).
- the invention provides a the method as describe above, comprising reducing or deleting the expression of at least one nucleic acid molecule, wherein said molecule is selected from: (a) a nucleic acid molecule encoding a EBIl polypeptide or EBI2 polypeptide; and (b) a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 (EBIl a), SEQ ID NO: 3 (EBIlb), SEQ ID NO: 6 (EBI2a); and SEQ ID NO: 8 (EBI2b).
- the method comprise the further step of transforming regenerable cells of a plant with said nucleic acid construct or recombinant DNA construct and regenerating a transgenic plant from said transformed cell.
- the nucleic acid to be inserted should be assembled within a construct that contains effective regulatory elements that will drive transcription, as described above. There must be available a method of transporting the construct into the cell. Once the construct is within the cell, integration into the endogenous chromosomal material either will or will not occur.
- Transformation techniques may be used to introduce the DNA constructs and vectors into plant cells to produce transgenic plants, in particular transgenic trees, with improved growth properties.
- host cells may be employed as recipients for the DNA constructs and vectors according to the invention.
- Non-limiting examples of host cells include cells in embryonic tissue, callus tissue type I, II, and III, hypocotyls, meristem, root tissue, tissues for expression in phloem, leaf discs, petioles and stem intemodes.
- Agrobacterium transformation is one method widely used by those skilled in the art to transform tree species, in particular hardwood species such as poplar. Production of stable, fertile transgenic plants is now a routine in the art.
- Other methods such as microprojectile or particle bombardment, electroporation, microinjection, direct DNA uptake, liposome mediated DNA uptake, or the vortexing method may be used where Agrobacterium transformation is inefficient or ineffective, for example in some gymnosperm species.
- a combination of different techniques may be employed to enhance the efficiency of the transformation process, e.g. bombardment with Agrobacterium coated microparticles or microprojectile bombardment to induce wounding followed by co-cultivation with Agrobacterium.
- transgenic plants are preferably selected using a dominant selectable marker incorporated into the transformation vector.
- a dominant selectable marker will confer antibiotic or herbicide resistance on the transformed plants and selection of transformants can be accomplished by exposing the plants to appropriate concentrations of the antibiotic or herbicide.
- a novel selection marker using the D-form of amino acids and based on the fact that plants can only tolerate the L- form offers a fast, efficient and environmentally friendly selection system. An interesting feature of this selection system is that it enables both selection and counter-selection.
- a plant may be regenerated, e.g. from single cells, callus tissue or leaf discs, as is standard in the art. Almost any plant can be entirely regenerated from cells, tissues and organs of the plant. After transformed plants are selected and grown to maturity, those plants showing altered growth properties phenotype are identified. Additionally, to confirm that the phenotype is due to changes in expression levels or activity of the polypeptide or polynucleotide disclosed herein can be determined by analyzing mRNA expression using Northern blots, RT-PCR or microarrays, or protein expression using immunoblots or Western blots or gel shift assays.
- the method according to the invention comprises at least one step selected from:
- nucleic acid molecule encoding a ribonucleic acid sequence, which is able to form a double-stranded ribonucleic acid molecule, whereby a fragment of at least 17 nucleotides (such as 18, 19, 20 or 21 nucleotides) of said double- stranded ribonucleic acid molecule has a nucleic acid sequence having at least 50 % (such as 60%, 70%, 80%, 90%, or 95%) nucleic acid sequence identity to an EBI (i.e. EBIla, EBIlb, EBI2a, or EBI2b) nucleic acid molecule;
- EBI i.e. EBIla, EBIlb, EBI2a, or EBI2b
- RNAi or antisense nucleic acid molecule comprises a fragment of at least 17 nucleotides (such as 18, 19, 20 or 21 nucleotides) with a nucleic acid sequence having at least 50 % (such as 60%, 70%, 80%, 90%, or 95%) nucleic acid sequence identity to an EBI nucleic acid molecule;
- a nucleic acid construct able to recombine with and silence, inactivate, or reduce the activity of an endogenous gene comprising an EBI nucleic acid molecule;
- the invention provides a method wherein reducing or deleting of the amount or activity of an EBI1 polypeptide or EBI2 polypeptide is caused by any one of:
- said endogenous gene comprises an EBI nucleic acid molecule.
- the method according to the invention comprises:
- the invention provides a genetically modified, especially a transgenic, plant produced by the methods as described above.
- the transgenic plant may be a perennial plant which preferable is a woody plant or a woody species.
- the woody plant is a hardwood plant which may be selected from the group consisting of acacia, eucalyptus, hornbeam, beech, mahogany, walnut, oak, ash, willow, hickory, birch, chestnut, poplar, alder, maple, sycamore, ginkgo, a palm tree and sweet gum.
- Hardwood plants from the Salicaceae family, such as willow, poplar and aspen including variants thereof, are of particular interest, as these two groups include fast-growing species of tree or woody shrub which are grown specifically to provide timber and bio-fuel.
- the woody plant is a conifer which may be selected from the group consisting of cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, pine, redwood, spruce and yew.
- the woody plant is a fruit bearing plant which may be selected from the group consisting of apple, plum, pear, banana, orange, kiwi, lemon, cherry, grapevine and fig.
- Other woody plants which may be useful in the present method may also be selected from the group consisting of cotton, bamboo and rubber plants.
- Other plants, which may be useful is grasses grown for biomass production, for example Miscanthus and Switchgrass.
- the present invention extends to any plant cell of the above transgenic plants obtained by the methods described herein, and to all plant parts, including harvestable parts of a plant, seeds and propagules thereof, and plant explant or plant tissue.
- the present invention also encompasses a plant, a part thereof, a plant cell or a plant progeny comprising a DNA construct according to the invention.
- the present invention extends further to encompass the progeny of a primary transformed or transfected cell, tissue, organ or whole plant that has been produced by any of the aforementioned methods, the only requirement being that progeny exhibit the same genotypic and/or phenotypic characteristic(s) as those produced in the parent by the methods according to the invention.
- the invention provides a genetically modified plant having improved growth properties as compared to a corresponding non-genetically modified wild type plant, wherein said plant has a reduced amount or activity of a EBI1 or EBI2 polypeptide, and wherein the genome of said plant comprises a genetic modification selected from any one of:
- a non-silent mutation in an endogenous gene comprising a nucleic acid molecule encoding an EBI1 or EBI2 polypeptide
- transgene inserted into said genome, said transgene comprising a nucleic acid molecule encoding a ribonucleic acid sequence, which is able to form a double-stranded ribonucleic acid molecule, whereby a fragment of at least 17 nucleotides of said double-stranded ribonucleic acid molecule has a homology of at least 50% to a nucleic acid molecule encoding an EBIl or EBI2 polypeptide;
- a mutation in an endogenous gene comprising a nucleic acid molecule encoding an EBIl or
- EBI2 polypeptide induced by introducing into at least one plant cell a nucleic acid construct able to recombine with and silence, inactivate, or reduce the activity of the endogenous gene, wherein said EBIl polypeptide has an amino acid sequence having at least 80% amino acid sequence identity to a sequence selected from among SEQ ID NOS: 2, 4 and 5, or wherein said EBI2 polypeptide has an amino acid sequence having at least 80% amino acid sequence identity to a sequence selected from among SEQ ID NOS: 7, 9 and 10.
- the invention provides the use of EBIl and EBI2 genes for the identification of plants having increased growth as compared to the wild-type.
- the invention provides the use of EBIl and EBI2 genes and polypeptides in the identification of agents useful for inhibiting EBIl or EBI2 activity, thereby being useful for improving plant growth.
- the invention provides the use of EBIl and EBI2 genes as candidate genes in marker assisted breeding.
- EBIl and EBI2 appear to have a light induced and diurnal expression with a circadian pattern e.g. EBI2, less clear so EBIl , when assayed every four hours under 48 h in an 18 h light/ 6 h dark day length regime (18°C/18°C) starting 3 h before dawn ( Figure 1, rows 1 and 2; Real Time PCR Biological repeat 1 and 2, each containing leaves sampled at 7-9 internodes from four independent trees at each time point) and in DIURNAL ( Figure 1, row 3; http :// d iurnal . cgrb. oregonstate. edu/ ' ) .
- EBIl and EBI2 are expressed in various tissues as found in Poplar eFP Browser (http ://bar.utoronto. ca/ef pop/cgi-bin''ef Web. cgi).
- RNAi trigger regions were amplified from Populus tremula x tremuloides cDNA by PCR using Platinum pfx DNA polymerase (Invitrogen, Carlsbad, CA, USA) according to the product manual with following primer sets:
- EBIl constructs were used to down -regulate EPIla and EPIlb, and the EBI2 constructs were used down-regulate EBI2a and EBI2b.
- PCR products were cloned in pENTRTM/SD/D-TOPO ® vector (Invitrogen, Carlsbad, CA, USA).
- pENTRTM/SD/D-TOPO ® vector Invitrogen, Carlsbad, CA, USA.
- these vectors were digested with Noil and self-ligated.
- These entry vectors were subjected to dideoxy-nucleotide sequencing and used in the LR-Gateway reaction (Invitrogen, Carlsbad, CA, USA) with the destination vector, pANDA35FIK.
- Agrobacterium mediated transformation was subsequently used in order to transform hybrid aspen, Populus tremula L. x P. tremuloides Mich. Clone T89 was transformed and regenerated according to methods known in the art.
- the transgenic poplar lines were grown together with their wildtype control (wt) trees, in a growth chamber under a photoperiod of 18h and a temperature of 18°C/18°C (day /night).
- the plants were fertilized weekly Weibulls Rika S NPK 7-1-5 diluted 1 to 100 (final concentrations N0 3 , 55g/l; NH 4 , 29g/l; P, 12g/l; K, 56g/l; Mg 7,2g/l; S, 7,2g/l; B, 0,18g/l; Cu, 0,02g/l; Fe, 0,84g/l; Mn, 0,42g/l; Mo, 0,03g/l; Zn, 0,13g/L). Height and diameter was measured and used for analysis of growth.
- EBIl transgenic trees show 25-28% increased volume growth index and 5-13% increased height. Some of the EBI2 transgenic trees show 15-36% volume growth index and 6-14% increased height.
- Ratios are the average of transgenic line replicates divided with average of wt values.
- Volume index are calculated as (diameter x diameter x height). The t-test values show the p-value.
Abstract
Description
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Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
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CA2865229A CA2865229A1 (en) | 2012-03-06 | 2013-03-05 | Plants having improved growth properties |
AU2013230864A AU2013230864B2 (en) | 2012-03-06 | 2013-03-05 | Plants having improved growth properties |
NZ629391A NZ629391A (en) | 2012-03-06 | 2013-03-05 | Plants having improved growth properties |
JP2014560889A JP6121457B2 (en) | 2012-03-06 | 2013-03-05 | Plants with improved growth characteristics |
EP13757085.9A EP2823046A4 (en) | 2012-03-06 | 2013-03-05 | Plants having improved growth properties |
CN201380012776.9A CN104169423A (en) | 2012-03-06 | 2013-03-05 | Plants having improved growth properties |
BR112014022054A BR112014022054A2 (en) | 2012-03-06 | 2013-03-05 | plants having improved growth properties |
US14/382,937 US20150033389A1 (en) | 2012-03-06 | 2013-03-05 | Plants having improved growth properties |
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US201261607404P | 2012-03-06 | 2012-03-06 | |
US61/607,404 | 2012-03-06 |
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PCT/SE2013/050191 WO2013133753A1 (en) | 2012-03-06 | 2013-03-05 | Plants having improved growth properties |
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US (1) | US20150033389A1 (en) |
EP (1) | EP2823046A4 (en) |
JP (1) | JP6121457B2 (en) |
CN (1) | CN104169423A (en) |
AU (1) | AU2013230864B2 (en) |
BR (1) | BR112014022054A2 (en) |
CA (1) | CA2865229A1 (en) |
CL (1) | CL2014002338A1 (en) |
NZ (1) | NZ629391A (en) |
UY (1) | UY34658A (en) |
WO (1) | WO2013133753A1 (en) |
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CA2970260A1 (en) * | 2014-12-29 | 2016-07-07 | Swetree Technologies Ab | Woody plants having improved growth properties |
CN116769799B (en) * | 2023-08-18 | 2023-11-10 | 南昌大学 | Soybean mutant gene for improving yield of leguminous crops and application thereof |
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WO2010045218A1 (en) * | 2008-10-13 | 2010-04-22 | Board Of Regents, The University Of Texas System | Molecular clock mechanism of hybrid vigor |
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- 2013-03-05 JP JP2014560889A patent/JP6121457B2/en not_active Expired - Fee Related
- 2013-03-05 WO PCT/SE2013/050191 patent/WO2013133753A1/en active Application Filing
- 2013-03-05 BR BR112014022054A patent/BR112014022054A2/en active Search and Examination
- 2013-03-05 US US14/382,937 patent/US20150033389A1/en not_active Abandoned
- 2013-03-05 CN CN201380012776.9A patent/CN104169423A/en active Pending
- 2013-03-05 NZ NZ629391A patent/NZ629391A/en not_active IP Right Cessation
- 2013-03-05 EP EP13757085.9A patent/EP2823046A4/en not_active Withdrawn
- 2013-03-05 CA CA2865229A patent/CA2865229A1/en not_active Abandoned
- 2013-03-06 UY UY0001034658A patent/UY34658A/en not_active Application Discontinuation
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2014
- 2014-09-03 CL CL2014002338A patent/CL2014002338A1/en unknown
Non-Patent Citations (7)
Title |
---|
ASHELFORD K. ET AL.: "Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis", GENOME BIOLOGY, vol. 12, 2011, pages R28, XP021097624 * |
JOHANSSON M. ET AL.: "Partners in Time: EARLY BIRD associates with ZEITLUPE and regulates the speed of the Arabidopsis clock", PLANT PHYSIOLOGY, vol. 155, 2011, pages 2108 - 2122, XP055164742 * |
JOHANSSON, M. ET AL.: "Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock", PLANT PHYSIOL., vol. 155, no. 4, 2011, pages 2108 - 2122, XP055164742, DOI: doi:10.1104/pp.110.167155 |
LISSO J. ET AL.: "NFX1-LIKE2 (NFXL2) suppresses abscisic acid accumulation and stomatal closure in Arabidopsis thaliana", PLOS ONE, vol. 6, no. E26982, November 2011 (2011-11-01), pages 1 - 12, XP055164734 * |
LISSO J. ET AL.: "The AtNFXLI gene encodes a NF-X1 type zinc finger protein required for growth under salt stress", FEBS LETTERS, vol. 580, 2006, pages 4851 - 4856, XP028061356 * |
ROSSO M. G. ET AL.: "An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics", PLANT MOLECULAR BIOLOGY, vol. 53, 2003, pages 247 - 259, XP002450842 * |
See also references of EP2823046A4 |
Also Published As
Publication number | Publication date |
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JP2015509377A (en) | 2015-03-30 |
US20150033389A1 (en) | 2015-01-29 |
BR112014022054A2 (en) | 2017-07-04 |
AU2013230864A1 (en) | 2014-09-18 |
AU2013230864B2 (en) | 2018-07-19 |
EP2823046A1 (en) | 2015-01-14 |
CN104169423A (en) | 2014-11-26 |
UY34658A (en) | 2013-10-31 |
JP6121457B2 (en) | 2017-04-26 |
CL2014002338A1 (en) | 2015-02-27 |
EP2823046A4 (en) | 2016-01-20 |
CA2865229A1 (en) | 2013-09-12 |
NZ629391A (en) | 2016-01-29 |
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