WO2013065498A1 - Fluorescent light phantom device and fluorescent light imaging method - Google Patents

Fluorescent light phantom device and fluorescent light imaging method Download PDF

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Publication number
WO2013065498A1
WO2013065498A1 PCT/JP2012/076976 JP2012076976W WO2013065498A1 WO 2013065498 A1 WO2013065498 A1 WO 2013065498A1 JP 2012076976 W JP2012076976 W JP 2012076976W WO 2013065498 A1 WO2013065498 A1 WO 2013065498A1
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Prior art keywords
fluorescent
phantom
phantoms
fluorescence
measurement object
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PCT/JP2012/076976
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French (fr)
Japanese (ja)
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健二郎 長谷川
光春 三輪
貴弘 鹿山
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浜松ホトニクス株式会社
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Priority to JP2011241650A priority Critical patent/JP6087049B2/en
Priority to JP2011-241650 priority
Application filed by 浜松ホトニクス株式会社 filed Critical 浜松ホトニクス株式会社
Publication of WO2013065498A1 publication Critical patent/WO2013065498A1/en

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J1/00Photometry, e.g. photographic exposure meter
    • G01J1/58Photometry, e.g. photographic exposure meter using luminescence generated by light
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Detecting, measuring or recording for diagnostic purposes; Identification of persons
    • A61B5/0059Detecting, measuring or recording for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071Detecting, measuring or recording for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Detecting, measuring or recording for diagnostic purposes; Identification of persons
    • A61B5/02Detecting, measuring or recording pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography or electroauscultation; Heart catheters for measuring blood pressure
    • A61B5/026Measuring blood flow
    • A61B5/0261Measuring blood flow using optical means, e.g. infra-red light
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B90/00Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/39Markers, e.g. radio-opaque or breast lesions markers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274Calibration, base line adjustment, drift correction
    • G01N21/278Constitution of standards
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B90/00Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/39Markers, e.g. radio-opaque or breast lesions markers
    • A61B2090/3937Visible markers
    • A61B2090/3941Photoluminescent markers
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2560/00Constructional details of operational features of apparatus; Accessories for medical measuring apparatus
    • A61B2560/02Operational features
    • A61B2560/0223Operational features of calibration, e.g. protocols for calibrating sensors
    • A61B2560/0228Operational features of calibration, e.g. protocols for calibrating sensors using calibration standards
    • A61B2560/0233Optical standards
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging

Abstract

A fluorescent light phantom device (1) comprises: a phantom support body (10) having fluorescent light phantom housing units (1b, 1c, 1d, 1e); and fluorescent light phantoms (12, 13, 14, 15) which are configured by including a fluorescent light pigment of a prescribed concentration in a medium which reproduces light diffusion and/or light absorption of an object to be measured, and which are housed in the fluorescent light phantom housing units (1b, 1c, 1d, 1e).

Description

Fluorescence phantom device and fluorescence imaging method

The present invention relates to a fluorescent phantom device and a fluorescent imaging method.

In the field of plastic surgery in the field of plastic surgery, it is known that the presence or absence of blood flow in the transplanted living tissue has a significant effect on the prognosis of the operation. As a method for determining the presence or absence of blood flow, a patient is injected with indocyanine green (hereinafter referred to as “ICG”) reagent, and then the tissue of interest is irradiated with near infrared light, and the tissue is observed with a camera. A method for determining the presence or absence of blood flow is known. In such a blood flow presence / absence determination method, for example, a calibration assistance means described in Patent Document 1 is known as a calibration assistance means for standardizing a measurement result. The calibration assisting means described in Patent Document 1 is produced by dissolving ICG dye and albumin protein in water and immersing them in a carrier sheet.

JP 2005-300540 A

However, the calibration assisting means described in Patent Document 1 has the following problems. That is, in the manufacturing process of the calibration assisting means, the solution containing the fluorescent dye is soaked in the carrier sheet such as paper or cloth, so that it is difficult to accurately adjust the concentration of the fluorescent dye. Further, in this calibration assisting means, since the fluorescent dye is bound to albumin protein, there is a concern about deterioration of the fluorescence characteristics accompanying protein denaturation. From the above points, it has been difficult to quantitatively evaluate the concentration of the fluorescent dye contained in the measurement object using such calibration means.

The present invention has been made to solve the above problems, and provides a fluorescent phantom device and a fluorescent imaging method capable of quantitatively evaluating the concentration of a fluorescent dye in a measurement object. Objective.

In order to achieve such an object, a fluorescent phantom device according to one aspect of the present invention is a phantom support having a fluorescent phantom housing portion and a medium that reproduces at least one of light scattering and light absorption of a measurement object. A fluorescent phantom configured to contain a fluorescent dye of a predetermined concentration and housed in the fluorescent phantom housing portion.

In the fluorescent phantom device according to one aspect of the present invention, since the fluorescent phantom having a predetermined concentration of the fluorescent dye is provided, the measurement object and the fluorescent phantom of the present invention are irradiated with near infrared light, By comparing and observing the fluorescence from the measurement object and the fluorescence brightness from the fluorescence phantom, the concentration of the fluorescence dye in the measurement object can be compared quantitatively with the brightness of the fluorescence dye in the fluorescence phantom. It becomes possible.

Further, in the fluorescent phantom device according to one aspect of the present invention, the phantom support has a plurality of fluorescent phantom accommodating portions, and each of the fluorescent phantom accommodating portions accommodates the fluorescent phantom, and the fluorescent phantom is contained in the fluorescent phantom. The concentration of the dye may be different in each fluorescent phantom. In this case, since the concentration of the fluorescent dye is different in each of the plurality of fluorescent phantoms, the fluorescent dye in the measurement object is obtained by comparing and observing the luminance of the fluorescence from the measurement object and the fluorescence from the plurality of fluorescent phantoms. It becomes possible to evaluate the density | concentration of more quantitatively.

In addition, a fluorescent phantom device according to one aspect of the present invention includes a phantom support having a fluorescent phantom accommodating portion, and a fluorescent phantom accommodated in the fluorescent phantom accommodating portion, and the fluorescent phantom is light scattering of a measurement object. And a surface layer dummy arranged on the surface layer of the fluorescent phantom accommodating portion, and a deep layer dummy arranged on the deep layer of the fluorescent phantom accommodating portion. And a plate-like phantom that is arranged in a fluorescent phantom accommodating portion while being sandwiched between a surface layer dummy and a deep layer dummy.

In the fluorescent phantom device according to one aspect of the present invention, a plate-like phantom is disposed between a surface layer dummy and a deep layer dummy made of a medium that reproduces light scattering and light absorption of a measurement object. Therefore, by irradiating the measurement object and the fluorescent phantom device of the present invention with near infrared light, and comparing the fluorescence from the measurement object and the fluorescence luminance from the fluorescence phantom, the skin and fat of the measurement object are observed. Even if scattering or light absorption occurs due to the difference in muscle thickness, the presence or absence of the fluorescent dye in the measurement object can be correctly evaluated.

In the fluorescent phantom device according to one aspect of the present invention, the phantom support includes a plurality of fluorescent phantom accommodating portions, and the fluorescent phantoms are accommodated in the plurality of fluorescent phantom accommodating portions, respectively. Each of the fluorescent phantoms may have an equivalent thickness, contain an equivalent concentration of the fluorescent dye, and be disposed at a different depth in each of the fluorescent phantoms. In this case, plate-like phantoms having the same thickness and containing the fluorescent dye having the same concentration are arranged at different depths in the respective fluorescent phantoms. In addition, a plate-like phantom is disposed between a surface layer dummy and a deep layer dummy that are formed of a medium that reproduces light scattering and light absorption of the measurement object. Therefore, by irradiating the measurement object and the fluorescent phantom device of the present invention with near infrared light, and comparing the fluorescence from the measurement object and the fluorescence luminance from the fluorescence phantom, the skin and fat of the measurement object are observed. Even when scattering and light absorption occur due to differences in muscle thickness, the concentration of the fluorescent dye in the measurement object can be more accurately evaluated.

In addition, the fluorescent phantom device according to one aspect of the present invention includes a plurality of fluorescent phantom storage units arranged in a row, and the fluorescent phantom device is arranged in a matrix by arranging the fluorescent phantom device in a matrix. The concentration of the fluorescent dye contained in the phantom is different for each column.

In the fluorescent phantom device according to one aspect of the present invention, the fluorescent phantom devices are arranged in a matrix by arranging the fluorescent phantom devices in which a plurality of fluorescent phantom accommodating portions are arranged in a single row, thereby forming a plate-like phantom. The concentration of the fluorescent dye contained in each differs for each column. Therefore, the measurement object and the fluorescent phantom device of the present invention are irradiated with near-infrared light, and the fluorescence from the measurement object and the fluorescence from the fluorescence phantom device are compared and observed. It becomes possible to evaluate concentration and depth more accurately.

In the fluorescent phantom device according to one aspect of the present invention, the phantom support and the fluorescent phantom may be formed of an epoxy resin. In this case, since the phantom support, the standard phantom, and the fluorescent phantom are formed of an epoxy resin and solidified, the medium does not evaporate, and the reliability of the fluorescent dye concentration is ensured for a long time.

Further, in the fluorescent phantom device according to one aspect of the present invention, the phantom support further includes a standard phantom housing portion, and is composed of a medium that reproduces at least one of light scattering and light absorption of the measurement object. You may further have the standard phantom accommodated in an accommodating part. In this case, by comparing the fluorescence luminance of a standard phantom composed of a medium that reproduces light scattering and light absorption of the measurement object and a fluorescent phantom composed of the medium containing a fluorescent dye, It becomes possible to more accurately evaluate the fluorescence luminance without being affected by light scattering and light absorption.

In the fluorescent phantom device according to one aspect of the present invention, the medium is at least one kind of scattering particles selected from titanium dioxide particles, silica particles, polymer fine particles, alumina, quartz glass fine particles and lipid fine particles, a pigment and a dye. And at least one light absorbing material selected from the group consisting of: In this way, light scattering and light absorption of the measurement object are reproduced with higher accuracy, and fluorescence luminance is measured with higher accuracy.

The fluorescent phantom device according to one aspect of the present invention is used by being attached to a measurement object, and contains a fluorescent dye having a predetermined concentration in a medium that reproduces at least one of light scattering and light absorption of the measurement object. The fluorescent phantom is constructed. In this case, since the fluorescent phantom device is used by being attached to the measurement object, the fluorescent phantom device can be bent along a curved surface, and the measurement is performed when the surface of the measurement object is a curved surface. By bending the fluorescent phantom device along the surface of the object, it is possible to measure the fluorescence luminance from the measurement object more accurately.

Further, in the fluorescent phantom device according to one aspect of the present invention, a plurality of fluorescent phantoms may be provided, and the concentration of the fluorescent dye contained in the fluorescent phantom may be different in each fluorescent phantom. In this case, since the concentration of the fluorescent dye is different in each of the plurality of fluorescent phantoms, the fluorescent dye in the measurement object is obtained by comparing and observing the luminance of the fluorescence from the measurement object and the fluorescence from the plurality of fluorescent phantoms. It becomes possible to evaluate the density | concentration of more quantitatively.

In the fluorescent phantom device according to one aspect of the present invention, the fluorescent phantom may be formed of a polyurethane resin or a silicon resin. In this case, since the fluorescent phantom is formed in a semi-solid gel, the fluorescent phantom device can be bent along the surface of the measurement object, and the fluorescence luminance from the measurement object can be measured more accurately. It becomes possible.

Further, in the fluorescent phantom device according to one aspect of the present invention, the fluorescent dye may be ICG. In this way, since the ICG having the optical characteristics that the excitation wavelength is 750 to 810 nm and the center of the fluorescence wavelength is 840 nm is used as the fluorescent dye, when fluorescence observation is performed using a living body as a measurement object, from 600 nm It is difficult to be affected by light absorption by blood that absorbs light having a short wavelength or water that absorbs light having a wavelength longer than 1000 nm. Further, ICG that is harmless to a living body can be injected into the living body, and fluorescence observation can be performed using the fluorescent phantom device according to one aspect of the present invention.

In addition, the fluorescence imaging method according to one aspect of the present invention introduces a fluorescent dye into a living body, arranges the fluorescent phantom device of the present invention in the vicinity of the living body, irradiates the living body and the fluorescent phantom device with excitation light, By detecting near-infrared fluorescence from the fluorescent dye introduced and the fluorescent dye contained in the fluorescent phantom.

According to the fluorescence imaging method according to one aspect of the present invention, the concentration of the fluorescent dye in the living body is irradiated with the fluorescent phantom device including the fluorescent phantom having a predetermined concentration of the fluorescent dye together with the living body of the measurement object. Can be quantitatively evaluated.

According to the present invention, a fluorescent phantom device and a fluorescent imaging method capable of quantitatively evaluating the concentration of a fluorescent dye in a measurement object are obtained.

It is a perspective view which shows the structure of the fluorescence phantom apparatus which concerns on 1st Embodiment. It is the photograph which image | photographed the fluorescence phantom apparatus which concerns on 1st Embodiment, and the biological body with the near-infrared camera. It is a perspective view which shows the structure of the fluorescence phantom apparatus which concerns on 2nd Embodiment. It is a top view which shows the structure of the fluorescence phantom apparatus which concerns on 3rd Embodiment. It is a schematic diagram which shows the usage method of the fluorescence phantom apparatus which concerns on 3rd Embodiment. It is a perspective view which shows the structure of the fluorescence phantom apparatus which concerns on 4th Embodiment. It is a perspective view which shows the structure of the fluorescence phantom apparatus which concerns on 5th Embodiment.

Hereinafter, a preferred embodiment of a fluorescent phantom device according to the present invention will be described with reference to the drawings. Note that, in each drawing, the same elements are denoted by the same reference numerals, and redundant description is omitted.
(First embodiment)

FIG. 1 is a perspective view showing a configuration of a fluorescent phantom device 1 according to the first embodiment. As shown in the figure, the fluorescent phantom device 1 includes a phantom support 10, a standard phantom 11, and a plurality (four in the present embodiment) of fluorescent phantoms 12, 13, 14, and 15. Is done.

The phantom support 10 includes a standard phantom housing portion 1a for housing the standard phantom 11 and a plurality (four in this embodiment) of fluorescent phantom housing portions for housing the fluorescent phantoms 12, 13, 14, and 15. 1b, 1c, 1d, and 1e. The phantom support 10 is made of an epoxy resin.

The standard phantom 11 is composed of a medium that reproduces light scattering and light absorption of a measurement object such as a living body. Specifically, the medium constituting the standard phantom 11 includes titanium dioxide (TiO 2 ) particles, silica particles, polymer fine particles, alumina (Al 2 O 3 ), and quartz glass fine particles as scattering particles for reproducing light scattering. , Comprising at least one particle selected from lipid microparticles. Specific examples of lipid fine particles include milk and Intralipid (registered trademark). The medium constituting the standard phantom 11 contains at least one substance selected from pigments and dyes as a light absorbing substance for reproducing light absorption. In the present embodiment, the medium constituting the standard phantom 11 is a liquid obtained by mixing the scattering particles and the light absorbing substance with ethanol as a solvent. The standard phantom 11 is accommodated in the standard phantom accommodating portion 1a of the phantom support 10 and sealed.

The fluorescent phantoms 12, 13, 14, and 15 are configured by containing a fluorescent dye having a predetermined concentration in the above medium. In this embodiment, ICG is used as the fluorescent dye. The concentration of ICG contained in the fluorescent phantoms 12, 13, 14, and 15 is different in the fluorescent phantoms 12, 13, 14, and 15, respectively. In the present embodiment, the fluorescent phantoms 12, 13, 14, and 15 are arranged in a line, and are arranged in the order from the lowest ICG content concentration to the highest. In this embodiment, the fluorescent phantoms 12, 13, 14, and 15 are liquids, and are accommodated in the fluorescent phantom accommodating portions 1b, 1c, 1d, and 1e of the phantom support 10 and sealed.

Next, a fluorescence imaging method using the fluorescence phantom device 1 of the present embodiment will be described. First, ICG is introduced into a living body as a measurement object as a fluorescent dye. Next, the fluorescent phantom device 1 is disposed in the vicinity of the living body. Then, the living body and the fluorescent phantom device 1 are irradiated with excitation light with near infrared light having an excitation wavelength of 750 nm to 810 nm. At this time, near-infrared fluorescence having a fluorescence wavelength centered at 840 nm is generated from ICG introduced into the living body. Further, near-infrared fluorescence of the same wavelength is also generated from the fluorescent phantoms 12, 13, 14, and 15 of the fluorescent phantom device 1. At this time, the intensity of the generated near-infrared fluorescence corresponds to the concentration of ICG contained in the living body and the fluorescent phantoms 12, 13, 14, and 15. The intensity of the generated near-infrared fluorescence increases as the concentration of ICG increases. Get higher. These near-infrared fluorescence is detected by, for example, a near-infrared camera. Fluorescence imaging processing can be performed by performing image processing on the detected near-infrared fluorescence by a known method.

FIG. 2 shows a photograph that is an example of the result of fluorescence imaging performed by the method described above. FIG. 2 is a photograph of the fluorescent phantom device 1 according to the first embodiment and a living body taken with a near-infrared camera. On the slightly upper side of the central portion in FIG. 2, the light spots are arranged in a line in the vertical direction. The brightness of these points is different, and the lower point is brighter. As described above, since the brightness of a point corresponds to the concentration of ICG in the fluorescent phantom, the lower the point, the brighter the point, the higher the concentration of ICG in the fluorescent phantom corresponding to the lower point. It represents that. Further, since the ICG content concentration contained in the fluorescent phantom is known, it is possible to associate the ICG content concentration with the point brightness from the brightness of the points arranged in a line. That is, the portion that shines with the same brightness has the same ICG concentration.

Further, in FIG. 2, a white shining portion spreads around the vertically arranged points representing the fluorescence from the fluorescent phantom. This represents fluorescence generated from ICG introduced into a living body as a measurement object. By comparing the fluorescence intensity from ICG in the living body with the fluorescence intensity of the fluorescent phantom, the magnitude relationship between the concentration of ICG in the living body and the concentration of ICG in the fluorescent phantom can be understood, and the concentration of ICG in the living body For example, the presence / absence of blood flow and the amount of blood flow while containing ICG in a living body can be quantitatively evaluated.

According to the present embodiment, since the fluorescent phantoms 12, 13, 14, and 15 having a predetermined concentration of the fluorescent dye are provided, the object to be measured and the fluorescent phantom device 1 of the present embodiment are connected to near infrared light. And observing the fluorescence from the measurement object and the fluorescence brightness from the fluorescence phantoms 12, 13, 14, and 15 to observe the concentration of the fluorescent dye in the measurement object. 15 can be quantitatively evaluated as compared with the luminance of the fluorescent dye in FIG.

Further, in the fluorescent phantom device 1, since the concentration of the fluorescent dye is different in each of the fluorescent phantoms 12, 13, 14, and 15, the fluorescence from the measurement object and the plurality of fluorescent phantoms 12, 13, 14, and 15 It is possible to more quantitatively evaluate the concentration of the fluorescent dye in the measurement object by comparing and observing the luminance of the fluorescence.

Further, since the fluorescent phantom device 1 includes the standard phantom 11, the standard phantom 11 including a medium that reproduces light scattering and light absorption of the measurement object and a fluorescent dye contained in the medium are configured. By comparing the fluorescence brightness with the fluorescence phantoms 12, 13, 14, and 15, the fluorescence brightness can be more accurately evaluated without being affected by light scattering and light absorption.

In the fluorescent phantom device 1, the medium constituting the standard phantom 11 and the fluorescent phantoms 12, 13, 14, and 15 is at least one selected from titanium dioxide particles, silica particles, polymer particles, alumina, quartz glass particles, and lipid particles. Since it contains seed scattering particles and at least one light-absorbing substance selected from pigments and dyes, the light scattering and light absorption of the measurement object can be reproduced more accurately, and the fluorescence luminance can be measured more accurately. Is done.

In addition, according to the fluorescence imaging method according to the present embodiment, in order to irradiate the fluorescence phantom device 1 including a plurality of fluorescence phantoms 12, 13, 14, and 15 having different concentrations of the fluorescent dye together with the living body of the measurement object, The concentration of the fluorescent dye in the living body can be quantitatively evaluated.

In addition, since the fluorescent phantom device 1 uses ICG as a fluorescent dye, ICG having the optical characteristics that the excitation wavelength is 750 to 810 nm and the center of the fluorescent wavelength is 840 nm is used as the fluorescent dye, so that the living body is measured. When performing fluorescence observation as a target object, it is difficult to be affected by light absorption by blood that absorbs light having a wavelength shorter than 600 nm or water that absorbs light having a wavelength longer than 1000 nm, and it is possible to observe the deep part of the living body. . Further, ICG that is harmless to the living body can be injected into the living body, and fluorescence observation can be performed using the fluorescent phantom device 1.

In the present embodiment, the solvent constituting the standard phantom and the fluorescent phantom need not be ethanol, and instead of ethanol, for example, methanol, dimethyl sulfoxide, water, or the like can be used. In addition, the medium constituting the standard phantom 11 and the fluorescent phantoms 12, 13, 14, and 15 need not include both the light scattering particles and the light absorbing material, and may include at least one.

(Second Embodiment)
Next, a second embodiment will be described. FIG. 3 is a perspective view showing the configuration of the fluorescent phantom device 2 according to the second embodiment. The fluorescent phantom device 2 according to the second embodiment is different from the fluorescent phantom device 1 according to the first embodiment in that a standard phantom 21 and fluorescent phantoms 22, 23, 24, and 25 are formed of an epoxy resin and solidified. It is different in point. Therefore, the difference from the first embodiment will be mainly described.

The fluorescent phantom device 2 includes a phantom support 20, a standard phantom 21, and fluorescent phantoms 22, 23, 24, and 25. The phantom support 20 includes a standard phantom housing portion 2a for housing the standard phantom 21 and fluorescent phantom housing portions 2b, 2c, 2d and 2e for housing the fluorescent phantoms 22, 23, 24 and 25.

The standard phantom 21 is composed of a medium that reproduces light scattering and light absorption, like the standard phantom 11 of the first embodiment. As the light scattering particles and the light absorbing material contained in this medium, the same materials as in the first embodiment can be used. In this embodiment, the medium is obtained by mixing light scattering particles and a light-absorbing substance with ethanol, and further mixing this liquid with an epoxy resin to solidify it.

The fluorescent phantoms 22, 23, 24, and 25 are configured by containing ICG having a predetermined concentration in the above medium. More specifically, the medium is formed by mixing light scattering particles and a light-absorbing substance in ethanol, further dissolving ICG of a predetermined concentration, and mixing and solidifying an epoxy resin. The concentration of ICG contained in the fluorescent phantoms 22, 23, 24, and 25 is different in each of the fluorescent phantoms 22, 23, 24, and 25. In the present embodiment, the fluorescent phantoms 22, 23, 24, and 25 are arranged in a line, and are arranged in order from the lowest ICG concentration to the highest.

Even when the fluorescence phantom device 2 configured as described above is used, the same fluorescence imaging method as that described in the first embodiment can be performed.

According to the present embodiment, the same effect as that according to the first embodiment can be obtained. Furthermore, according to the fluorescent phantom device 2, since the standard phantom 21 and the fluorescent phantoms 22, 23, 24, 25 are formed of an epoxy resin, the standard phantom 21 and the fluorescent phantoms 22, 23, 24, 25 are solidified. The medium does not evaporate, and the reliability of the fluorescent dye concentration is ensured over a long period of time.

(Third embodiment)
Next, a third embodiment will be described. FIG. 4 is a plan view showing the configuration of the fluorescent phantom device 3 according to the third embodiment. The fluorescent phantom device 3 according to the third embodiment includes a standard phantom 31 and fluorescent phantoms 32 and 33. The fluorescent phantom device 3 is used by being attached to a measurement object.

The standard phantom 31 is composed of a medium that reproduces light scattering and light absorption of the measurement object. Specifically, this medium is a mixture of ethanol mixed with light scattering particles and a light-absorbing substance similar to that in the first embodiment, in a polyurethane resin. Since polyurethane resin is mixed in the medium, the standard phantom 31 is semi-solidified and formed into a gel.

The fluorescent phantoms 32 and 33 are configured by containing ICG having a predetermined concentration in the above medium. Therefore, similarly to the standard phantom 31, the fluorescent phantoms 32 and 33 are also semi-solidified and formed in a gel form.

Since the fluorescent phantom device 3 is configured as described above, the fluorescent phantom device 3 is semi-solid that can be bent along a curved surface.

A method of using the fluorescent phantom device 3 configured as described above will be described with reference to FIG. FIG. 5 is a schematic diagram showing a method of using the fluorescent phantom device 3 according to the third embodiment. The surface of the living body B, which is the measurement object, forms a convex curved surface. The fluorescent phantom device 3 is bent along the surface of the living body B, and the fluorescent phantom device 3 is placed on the living body B. Then, the near-infrared camera P is brought close to a direction perpendicular to the surface of the fluorescent phantom device 3, and the fluorescent phantom device 3 and the living body are photographed by the near-infrared camera P.

Also with the fluorescent phantom device 3 configured as described above, the same effect as that obtained when the fluorescent phantom device 1 according to the first embodiment is used can be obtained. In the fluorescent phantom device 3, since the standard phantom 31 and the fluorescent phantoms 32, 33, 34, and 35 are formed of polyurethane resin, the fluorescent phantom device can be bent along a curved surface. When the surface of the object is a curved surface, the fluorescence luminance from the object to be measured can be measured more accurately by bending the fluorescence phantom device along the surface of the object to be measured.

In the present embodiment, the resin used for semi-solidifying the fluorescent phantom device 3 to form a gel is not a polyurethane resin but may be a silicon resin, for example.

(Fourth embodiment)
Next, a fourth embodiment will be described. FIG. 6 is a perspective view showing the configuration of the fluorescent phantom device 4 according to the fourth embodiment. The fluorescent phantom device 4 according to the fourth embodiment includes a phantom support 40, a standard phantom 41, and a plurality (four in this embodiment) of fluorescent phantoms 42, 43, 44, 45. The The phantom support 40 includes a standard phantom housing portion 4a for housing the standard phantom 41, and fluorescent phantom housing portions 4b, 4c, 4d, and 4e for housing the fluorescent phantoms 42, 43, 44, and 45.

As with the standard phantom 21 of the second embodiment, the standard phantom 41 is made of a medium in which an epoxy resin is mixed with ethanol in which light scattering particles and a light absorbing material are mixed, and is formed by solidification.

The fluorescent phantom 42 has a surface layer dummy 42A, a plate-like phantom 42B, and a deep layer dummy 42C. The surface layer dummy 42A is formed of a medium that reproduces light scattering and light absorption of the measurement object. Further, the surface layer dummy 42 </ b> A is disposed on the surface layer of the fluorescent phantom accommodating portion 4 b provided on the phantom support 40. The deep layer dummy 42C is made of the above-described medium, and is disposed in the deep layer of the fluorescent phantom accommodating portion 4b. The plate-like phantom 42B is configured by containing ICG as a fluorescent dye in the above medium. The plate-like phantom 42B is disposed in the fluorescent phantom accommodating portion 4b so as to be sandwiched between the surface layer dummy 42A and the deep layer dummy 42C.

Similarly, the fluorescent phantom 43 includes a surface layer dummy 43A, a plate-like phantom 43B, and a deep layer dummy 43C, and the plate-like phantom 43B is disposed in the fluorescent phantom accommodating portion 4c so as to be sandwiched between the surface layer dummy 43A and the deep layer dummy 43C. . The fluorescent phantom 44 includes a surface layer dummy 44A, a plate-like phantom 44B, and a deep layer dummy 44C, and the plate-like phantom 44B is disposed in the fluorescent phantom accommodating portion 4d so as to be sandwiched between the surface layer dummy 44A and the deep layer dummy 44C. The fluorescent phantom 45 includes a surface layer dummy 45A and a plate-like phantom 45B. In the fluorescent phantom 45, the plate-like phantom 45B is disposed at the deepest part of the fluorescent phantom accommodating portion 4e and does not have a deep layer dummy.

In the present embodiment, the plate-like phantoms 42B, 43B, 44B, and 45B have the same thickness in each of the fluorescent phantoms 42, 43, 44, and 45, and contain the same concentration of ICG. The fluorescent phantoms are arranged at different depths. In the present embodiment, the fluorescent phantoms 42, 43, 44, 45 are arranged in a line, and the depths at which the plate-like phantoms 42B, 43B, 44B, 45B are arranged are configured to increase in order. .

Even if the fluorescence phantom device 4 configured as described above is used, the same fluorescence imaging method as that described in the first embodiment can be performed.

According to the present embodiment, the plate-like phantom 42B is disposed between the surface layer dummy 42A and the deep layer dummy 42C that are made of a medium that reproduces light scattering and light absorption of the measurement object. Therefore, the skin of the measurement object is irradiated by irradiating the measurement object and the fluorescent phantom device 4 of the present embodiment with near-infrared light and comparing the fluorescence from the measurement object and the fluorescence luminance from the fluorescence phantom for observation. Even if scattering or light absorption occurs due to differences in the thickness of fat, muscle, or muscle, the presence or absence of a fluorescent dye in the measurement object can be correctly evaluated.

In this case, in the fluorescent phantom device 4 according to the present embodiment, the plate-like phantoms 42B, 43B, 44B, and 45B having the same thickness and containing the fluorescent dye having the same concentration are used as the fluorescent phantom. 42, 43, 44 and 45 are arranged at different depths. Further, a plate-like phantom 42B is disposed between a surface layer dummy 42A and a deep layer dummy 42C that are made of a medium that reproduces light scattering and light absorption of the measurement object. Therefore, the measurement object and the fluorescent phantom device 4 of the present embodiment are irradiated with near-infrared light, and the fluorescence from the measurement object and the fluorescence luminance from the fluorescence phantoms 42, 43, 44, 45 are compared and observed. Thus, even when scattering or light absorption occurs due to differences in the thickness of the skin, fat, or muscle of the measurement object, the concentration of the fluorescent dye in the measurement object can be more accurately evaluated.

(Fifth embodiment)
Next, a fifth embodiment will be described. FIG. 7 is a perspective view showing the configuration of the fluorescent phantom device 4 according to the fifth embodiment. The fluorescent phantom device 5 according to the fifth embodiment includes a phantom support 50, standard phantoms 51, 61, 71, and fluorescent phantoms 52, 53, 54, 55, 62, 63, 64, 65, 72, 73, 74. , 75. The standard phantoms 51, 61, 71 are formed of a medium in which an epoxy resin is mixed with ethanol in which light scattering particles and a light absorbing material are mixed, and is formed by solidification, similarly to the standard phantom 41 according to the fourth embodiment. Yes.

The fluorescent phantoms 52, 53, 54, 55, 62, 63, 64, 65, 72, 73, 74, and 75 are respectively plate-like phantoms similar to the fluorescent phantoms 42, 43, 44, and 45 according to the fourth embodiment. have. In the fluorescent phantom device 5, the standard phantom 51 and the fluorescent phantoms 52, 53, 54, and 55 are arranged in a line, the standard phantom 61 and the fluorescent phantoms 62, 63, 64, and 65 are arranged in a line, and the standard phantom 71 And fluorescent phantoms 72, 73, 74 and 75 are arranged in a line. The plate-like phantoms included in the fluorescent phantoms 52, 53, 54, and 55 have the same thickness, contain the same concentration of ICG, and are provided at different depths. The plate-like phantoms of the fluorescent phantoms 62, 63, 64, 65 have the same thickness, contain ICG of the same concentration, and are provided at different depths. The plate-like phantoms of the fluorescent phantoms 72, 73, 74, and 75 have the same thickness, contain the same concentration of ICG, and are provided at different depths. Here, the concentrations of ICG contained in the fluorescent phantoms 52, 62, and 72 are different from each other. Therefore, the phantom support body 50, the standard phantom 51, and the fluorescent phantoms 52, 53, 54, and 55 constitute the phantom device 5A of the fourth embodiment. Similarly, the phantom support 50, the standard phantom 61, and the fluorescent phantoms 62, 63, 64, 65 constitute the phantom device 5B of the fourth embodiment, and the phantom support 50, the standard phantom 71, the fluorescent phantom 72, 73, 74, and 75 constitute the phantom device 5C of the fourth embodiment. That is, the fluorescent phantom device 5 of the present embodiment can be regarded as a device in which the three fluorescent phantom devices 5A to 5C described above are arranged in a plurality of rows (in this embodiment, three rows are arranged). In the fluorescent phantom device 5, the three fluorescent phantom devices 5A to 5C are arranged so that the fluorescent phantoms 52, 53, 54, 55, 62, 63, 64, 65, 72, 73, 74, 75 are provided. It will be arranged in a matrix. Further, the concentration of ICG contained in each fluorescent phantom is different for each column.

Even when the fluorescence phantom device 5 configured as described above is used, the same fluorescence imaging method as that described in the first embodiment can be performed.

According to the present embodiment, the fluorescent phantom devices are arranged in a matrix by arranging a plurality of fluorescent phantom devices in which a plurality of fluorescent phantom accommodating portions are arranged in a row, and the concentration of the fluorescent dye contained in the plate-like phantom is The fluorescent phantom device 5 is configured differently for each column. Therefore, the measurement object and the fluorescent phantom device 5 of the present embodiment are irradiated with near-infrared light, and the fluorescence from the measurement object and the fluorescence from the fluorescence phantom device 5 are compared and observed. It becomes possible to more accurately evaluate the concentration and depth of the fluorescent dye.

According to the present invention, there are provided fluorescence phantom devices 1 to 5 and a fluorescence imaging method capable of quantitatively evaluating the concentration of a fluorescent dye in a measurement object.

1, 2, 3, 4, 5, 5A to 5C... Fluorescent phantom device, 1a, 2a, 4a... Standard phantom accommodating portion, 1b to 1e, 2b to 2e, 4b to 4e. 40, 50 ... phantom support, 11, 21, 31, 41, 51 ... standard phantom, 12, 13, 14, 15, 22, 23, 24, 25, 32, 33, 42, 43, 44, 45, 52 , 53, 54, 55, 62, 63, 64, 65, 72, 73, 74, 75 ... fluorescent phantom, 42A, 43A, 44A, 45A ... surface layer dummy, 42B, 43B, 44B, 45B ... plate phantom, 42C , 43C, 44C ... deep dummy, B ... living body, P ... near infrared camera.

Claims (13)

  1. A phantom support having a fluorescent phantom housing;
    A fluorescent phantom that is configured to contain a fluorescent dye of a predetermined concentration in a medium that reproduces at least one of light scattering and light absorption of the measurement object, and is accommodated in the fluorescent phantom accommodating portion;
    A fluorescent phantom device.
  2. The phantom support has a plurality of the fluorescent phantom accommodating portions,
    The fluorescent phantoms are housed in the plurality of fluorescent phantom housing parts,
    2. The fluorescent phantom device according to claim 1, wherein the concentration of the fluorescent dye contained in the fluorescent phantom is different in each of the fluorescent phantoms.
  3. A phantom support having a fluorescent phantom housing;
    A fluorescent phantom housed in the fluorescent phantom housing section;
    With
    The fluorescent phantom is
    It is composed of a medium that reproduces at least one of light scattering and light absorption of the measurement object, and a surface layer dummy disposed on the surface layer of the fluorescent phantom housing part
    A deep dummy disposed in the deep layer of the fluorescent phantom containing portion, and comprising the medium;
    A plate-like phantom that is configured to contain a fluorescent dye in the medium, and is disposed in the fluorescent phantom accommodating portion sandwiched between the surface layer dummy and the deep layer dummy,
    A fluorescent phantom device.
  4. The phantom support has a plurality of the fluorescent phantom accommodating portions,
    The fluorescent phantoms are housed in the plurality of fluorescent phantom housing parts,
    The plate-like phantom has an equivalent thickness in each of the fluorescent phantoms, contains the fluorescent dye having an equivalent concentration, and is disposed at a different depth in each of the fluorescent phantoms. Item 4. The fluorescent phantom device according to Item 3.
  5. The fluorescent phantom devices are arranged in a matrix by arranging the fluorescent phantom devices according to claim 4, wherein the fluorescent phantom accommodating portions are arranged in a row,
    The fluorescent phantom device characterized in that the concentration of the fluorescent dye contained in the plate-like phantom is different for each column.
  6. The fluorescent phantom device according to any one of claims 1 to 5, wherein the phantom support and the fluorescent phantom are formed of an epoxy resin.
  7. The phantom support further has a standard phantom receiving part,
    The fluorescent phantom device according to any one of claims 1 to 6, further comprising a standard phantom made of the medium and housed in the standard phantom housing portion.
  8. The medium is
    At least one kind of scattering particles selected from titanium dioxide particles, silica particles, polymer particles, alumina, quartz glass particles and lipid particles;
    At least one light absorbing material selected from pigments and dyes;
    The fluorescent phantom device according to any one of claims 1 to 7, characterized by comprising:
  9. Fluorescent phantom device provided with a fluorescent phantom that is used by being attached to a measurement object and containing a fluorescent dye having a predetermined concentration in a medium that reproduces at least one of light scattering and light absorption of the measurement object.
  10. The fluorescent phantom device according to claim 9, wherein a plurality of the fluorescent phantoms are provided, and the concentration of the fluorescent dye contained in the fluorescent phantom is different in each of the fluorescent phantoms.
  11. The fluorescent phantom device according to claim 9 or 10, wherein the fluorescent phantom is made of polyurethane resin or silicon resin.
  12. The fluorescent phantom device according to any one of claims 1 to 11, wherein the fluorescent dye is indocyanine green.
  13. A fluorescent dye is introduced into a living body, the fluorescent phantom device according to any one of claims 1 to 12 is disposed in the vicinity of the living body, the living body and the fluorescent phantom device are irradiated with excitation light, and the living body is irradiated. A fluorescence imaging method by detecting near-infrared fluorescence from an introduced fluorescent dye and a fluorescent dye contained in the fluorescent phantom.
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EP2775289A1 (en) 2014-09-10
CN103917857A (en) 2014-07-09
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US20140303496A1 (en) 2014-10-09

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