WO2013062900A1 - Inhibitors of the renal outer medullary potassium channel - Google Patents

Inhibitors of the renal outer medullary potassium channel Download PDF

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Publication number
WO2013062900A1
WO2013062900A1 PCT/US2012/061296 US2012061296W WO2013062900A1 WO 2013062900 A1 WO2013062900 A1 WO 2013062900A1 US 2012061296 W US2012061296 W US 2012061296W WO 2013062900 A1 WO2013062900 A1 WO 2013062900A1
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mmol
oxo
compound
pharmaceutically acceptable
carbonitrile
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PCT/US2012/061296
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French (fr)
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Haifeng Tang
Barbara Pio
Harry R. Chobanian
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Merck Sharp & Dohme Corp.
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Priority to EP12844384.3A priority Critical patent/EP2771004B1/en
Priority to US14/353,418 priority patent/US8999991B2/en
Publication of WO2013062900A1 publication Critical patent/WO2013062900A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
    • C07D241/04—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having no double bonds between ring members or between ring members and non-ring members
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/06—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems

Definitions

  • ROMK Renal Outer Medullary Potassium channel
  • ROMK participates in potassium recycling across the luminal membrane which is critical for the function of the Na + /K + /2C1 " co-transporter, the rate- determining step for salt reuptake in this part of the nephron.
  • ROMK provides a pathway for potassium secretion that is tightly coupled to sodium uptake through the amiloride- sensitive sodium channel (see Reinalter, S.C., et al., Pharmacotyping of hypokalaemic salt-losing tubular disorders, Acta Physiol Scand, 2004, 181(4): p.
  • ROMK channel also referred to herein as inhibitors of ROMK or ROMK inhibitors
  • ROMK inhibitors are predicted to represent novel diuretics for the treatment of hypertension and other conditions where treatment with a diuretic would be beneficial with potentially reduced liabilities (i.e., hypo- or hyperkalemia, new onset of diabetes, dyslipidemia) over the currently used clinical agents (see Lifton, R.P., A.G. Gharavi, and D.S. Geller, Molecular mechanisms of human hypertension, Cell, 2001, 104(4): p. 545-56).
  • Patent application publication number WO2010/129379 published November 11, 2010 having common representative Merck Sharp & Dohme Corp., (also published as
  • R5 and R6 are independently -H, -Ci-6 alkyl, -C3-6 cycloalkyl, -CF3, -CHF2, -C3 ⁇ 4F or
  • X is -H, -OH,-OCi-3alkyl, -F, oxo, NH 2 or-CH3; and Xl is -H or -CH3.
  • Patent application publication number WO2012/058134 published May 3, 2012, having common representative Merck Sharp & Dohme Corp., describes ROMK inhibitors having the generic formula:
  • a and B are mono and/or bicyclic aromatic groups; R2 is -H,
  • R3 is -H, -Ci-6 alkyl, -C3.6 cycloalkyl, -OH, -F, -OC1.3 alkyl, or -CH2OH, or R3 can be joined to R 10b to form a ring.
  • Patent application publication number WO2012/058116 published May 3, 2012, having common representative Merck Sharp & Dohme Corp., describes ROMK inhibitors having the generic formula: wherein R5 and R6 are independently -H, -Ci-6 alkyl or -C(0)OCi-3alkyl; and X, X 1 , Y and Y 1 are independently -H or-Ci-6alkyl; or Yl can be joined together with Z2 to form a fused ring system.
  • the compounds of Formula I and salts thereof of this invention are selective inhibitors of the ROMK channel and could be used for the treatment of hypertension, heart failure and other conditions where treatment with a diuretic or natriuretic would be beneficial.
  • the present invention provides compounds of Formula I, defined herein, and the pharmaceutically acceptable salts thereof.
  • the compounds of Formula I are inhibitors of the ROMK (Kirl.l) channel.
  • the compounds of Formula I could be used in methods of treatment, inhibition or amelioration of one or more disease states that could benefit from inhibition of ROMK.
  • the compounds of this invention could be used in methods of treatment which comprise administering a therapeutically or prophylactically effective amount of a compound of Formula I to a patient in need of a diuretic and/or natriuretic agent.
  • the compounds of Formula I could be valuable pharmaceutically active compounds for the therapy, prophylaxis or both of medical conditions, including, but not limited to, cardiovascular diseases such as hypertension and heart failure, and conditions associated with excessive salt and water retention.
  • cardiovascular diseases such as hypertension and heart failure
  • the compounds of this invention could further be used in combination with other therapeutically effective agents, including but not limited to, other drugs which are useful for the treatment of hypertension, heart failure and conditions associated withexcessive salt and water retention.
  • the invention furthermore relates to processes for preparing compounds of Formula I, and pharmaceutical compositions which comprise compounds of Formula I.
  • the present invention is directed to compounds having structural Formula I:
  • dashed bonds represent the optional presence of -Ci_2alkanediyl- which, when present,
  • Rl is -H, -CH3, -CF3, -CHF2, -CH2F or -CH2OH;
  • R4 is -H, -OH, oxo or -C l -6alkyl
  • R4 is -OH or oxo, then R2 is not oxo;
  • R3 and R5 are each independently -H or -Ci_6alkyl; provided that Its is absent when R2 is oxo, and R5 is absent when R is oxo;
  • Rla is -H, halo or -Ci_3alkyl
  • Rlb is -H, halo, -Ci-3alkyl, -O-Ci alkyl or -COOCi-3alkyl;
  • R2a and R2b 1S -CN and the other is -H or -Ci-3alkyl
  • R2c and R2d 1S -S02-Ci-3alkyl and the other is -H or -Ci-3alkyl;
  • Xl, X , ⁇ 3 and X4 are each independently CH or N, provided that only one of ⁇ , ⁇ 2, ⁇ 3 or ⁇ is N;
  • X5, ⁇ 6 and X 7 are each independently CH or N, provided that only one of X5, ⁇ 6 0 r X 7 is N;
  • Ra and Rb are each independently -H, halo, -Ci_3alkyl, -0-Ci-3alkyl or -COOCi-3alkyl.
  • the present invention is further directed to compounds of Formula I having structural Formula ⁇ :
  • compounds of Formula I or ⁇ wherein Zl is zl-a are compounds of Formula I or ⁇ wherein Zl is zl-a.
  • R2, R3 and R5 are each -H, and R4 is -H, -OH or oxo, and more particularly R4 is -H or -OH.
  • Rl is -H.
  • compounds of Formula I or II wherein Zl is zl-b are compounds of Formula I or II wherein Zl is zl-b.
  • R2, R3 and R5 are each -H, and R4 is -H, -OH or oxo, and more particularly R4 is -H or -OH.
  • Rl is -H.
  • 2alkanediyl- and z 1 -c has the formula z 1 -c 1 :
  • alkyl is intended to include both branched- and straight-chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms. Commonly used abbreviations for alkyl groups are used throughout the specification.
  • Ci-6 alkyl (or “C1-C6 alkyl”), means linear or branched chain alkyl groups, including all isomers, having the specified number of carbon atoms and includes all of the hexyl and pentyl isomers as well as n-, iso-, sec- and tert-butyl (butyl, s-butyl, /-butyl, t-butyl;
  • Halo means -F, -CI, -Br and -I.
  • preferred halos are -F and -CI.
  • alkanediyl is a saturated aliphatic divalent hydrocarbon group having the specified number of carbon atoms.
  • alkanediyl groups include, but are not limited to, methanediyl, ethanediyl, propanediyl, butanediyl and the like.
  • the present invention encompasses all stereoisomeric forms of the compounds of Formula I. Centers of asymmetry that are present in the compounds of Formula I can all independently of one another have (R) or (S) configuration. When bonds to the chiral carbon are depicted as straight lines in the structural Formulas of the invention, or when a compound name is recited without a chiral designation for a chiral carbon, it is understood that both the (R) and (S) configurations of the chiral carbon and hence both enantiomers and mixtures thereof, are embraced within the Formula or by the name.
  • the production of specific stereoisomers or mixtures thereof may be identified in the Examples where such stereoisomers or mixtures were obtained, but this in no way limits the inclusion of all stereoisomers and mixtures thereof from being within the scope of this invention.
  • the invention includes all possible enantiomers and diastereomers and mixtures of two or more stereoisomers, for example mixtures of enantiomers and/or diastereomers, in all ratios.
  • enantiomers are a subject of the invention in enantiomerically pure form, both as levorotatory and as dextrorotatory antipodes, in the form of racemates and in the form of mixtures of the two enantiomers in all ratios.
  • the invention includes both the cis form and the trans form as well as mixtures of these forms in all ratios.
  • the preparation of individual stereoisomers can be carried out, if desired, by separation of a mixture by customary methods, for example by chromatography or crystallization, by the use of stereochemically uniform starting materials for the synthesis or by stereoselective synthesis.
  • a derivatization can be carried out before a separation of stereoisomers.
  • the separation of a mixture of stereoisomers can be carried out at an intermediate step during the synthesis of a compound of Formula I or it can be done on a final racemic product.
  • Absolute stereochemistry may be determined by X-ray crystallography of crystalline products or crystalline intermediates which are derivatized, if necessary, with a reagent containing a stereogenic center of known configuration.
  • Reference to the compounds of Formula I herein encompasses the compounds of Formula II and all embodiments thereof.
  • Reference to the compounds of this invention as those of a specific formula or embodiment or any other generic structural formula or specific compound described or claimed herein, is intended to encompass the specific compound or compounds falling within the scope of the Formula or embodiment, including salts thereof, particularly pharmaceutically acceptable salts, solvates (including hydrates) of such compounds and solvated (or hydrated) salt forms thereof, where such forms are possible unless specified otherwise.
  • the atoms may exhibit their natural isotopic abundances, or one or more of the atoms may be artificially enriched in a particular isotope having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number predominantly found in nature.
  • the present invention is meant to include all suitable isotopic variations of the compounds of Formula I.
  • different isotopic forms of hydrogen (H) include protium (lH) and deuterium (2H).
  • Protium is the predominant hydrogen isotope found in nature. Enriching for deuterium may afford certain therapeutic advantages, such as increasing in vivo half-life or reducing dosage requirements, or may provide a compound useful as a standard for characterization of biological samples.
  • Isotopically-enriched compounds within Formula I can be prepared without undue experimentation by conventional techniques well known to those skilled in the art or by processes analogous to those described in the Schemes and Examples herein using appropriate isotopically-enriched reagents and/or intermediates.
  • the invention also includes the corresponding pharmaceutically acceptable salts.
  • the compounds of Formula I which contain acidic groups can be used according to the invention, for example, as alkali metal salts, alkaline earth metal salts or as ammonium salts.
  • such salts include but are not limited to sodium salts, potassium salts, calcium salts, magnesium salts or salts with ammonia or organic amines such as, for example, ethylamine, ethanolamine, triethanolamine or amino acids.
  • Compounds of Formula I which contain one or more basic groups i.e.
  • the invention also includes, in addition to the salt forms mentioned, inner salts or betaines (zwitterions). Salts can be obtained from the compounds of Formula I by customary methods which are known to the person skilled in the art, for example by combination with an organic or inorganic acid or base in a solvent or dispersant, or by anion exchange or cation exchange from other salts.
  • the present invention also includes all salts of the compounds of Formula I which, owing to low physiological
  • compounds of the present invention may exist in amorphous form and/or one or more crystalline forms, and as such all amorphous and crystalline forms and mixtures thereof of the compounds of Formula I are intended to be included within the scope of the present invention.
  • some of the compounds of the instant invention may form solvates with water (i.e., a hydrate) or common organic solvents.
  • solvates and hydrates, particularly the pharmaceutically acceptable solvates and hydrates, of the instant compounds are likewise encompassed within the scope of this invention, along with un-solvated and anhydrous forms.
  • esters can optionally be made by esterification of an available carboxylic acid group or by formation of an ester on an available hydroxy group in a compound.
  • labile amides can be made.
  • Pharmaceutically acceptable esters or amides of the compounds of this invention may be prepared to act as pro-drugs which can be hydrolyzed back to an acid (or -COO- depending on the pH of the fluid or tissue where conversion takes place) or hydroxy form particularly in vivo and as such are encompassed within the scope of this invention.
  • Examples of pharmaceutically acceptable pro-drug modifications include, but are not limited to, -Ci -6alkyl esters and -C i -6alkyl substituted with phenyl esters.
  • the compounds within the generic structural formulas, embodiments and specific compounds described and claimed herein encompass salts, all possible stereoisomers and tautomers, physical forms (e.g., amorphous and crystalline forms), solvate and hydrate forms thereof and any combination of these forms, as well as the salts thereof, pro-drug forms thereof, and salts of pro-drug forms thereof, where such forms are possible unless specified otherwise.
  • the compounds of Formula I according to the invention are inhibitors of ROMK, and therefore could be used as diuretic and/or natriuretic agents.
  • ROMK inhibitors may be used to help to increase urination and increase urine volume and also to prevent or reduce reabsorption of sodium in the kidneys leading to increased excretion of sodium and water. Therefore, the compounds could be used for treatment or prophylaxis or both of disorders that benefit from increased excretion of water and sodium from the body. Accordingly, the compounds of this invention could be used in a method for inhibiting ROMK comprising administering a compound of Formula I in a ROMK-inhibitory effective amount to a patient in need thereof.
  • the inhibition of ROMK by the compounds of Formula I can be examined, for example, in the Thallium Flux Assay described below.
  • this invention also relates to the use of the compounds of Formula I or salts thereof to validate in vitro assays, for example but not limited to the Thallium Flux Assay described herein.
  • the compounds of this invention could be used in a method for causing diuresis, natriuresis or both, comprising administering a compound of Formula I in a therapeutically effective amount to a patient in need thereof.
  • the compounds of Formual I of this invention could be used in methods for treatment of, prevention of or reduction of risk for developing medical conditions that benefit from increased excretion of water and sodium, such as but not limited to one or more of hypertension, heart failure (both acute and chronic, the latter also known as congestive heart failure) and/or other conditions associated with excessive salt and water retention.
  • medical conditions such as but not limited to one or more of hypertension, heart failure (both acute and chronic, the latter also known as congestive heart failure) and/or other conditions associated with excessive salt and water retention.
  • the compounds of Formula I could be used in methods for treatment of, prevention of or reduction of risk for developing one or more disorders such as pulmonary arterial hypertension (PAH), cardiovascular disease, diabetes mellitus, diabetes insipidus, post-operative volume overload, endothelial dysfunction, diastolic dysfunction, systolic dysfunction, stable and unstable angina pectoris, thromboses, restenosis, myocardial infarction, stroke, cardiac insufficiency, pulmonary hypertonia, atherosclerosis, hepatic cirrhosis, ascitis, pre-eclampsia, cerebral edema, nephropathy, glomerulonephritis, nephrotic syndrome, acute and chronic kidney insufficiency (also referred to as chronic kidney disease, or more generally as renal impairment), acute tubular necrosis, hypercalcemia, idiopathic edema, Dent's disease, Meniere's disease, edematous states, glaucoma, benign intra
  • the compounds of Formula I may potentially have reduced liabilities (for example, hypo- or hyperkalemia, new onset of diabetes, dyslipidemia, etc.) over currently used clinical agents. Also the compounds may have reduced risk for diuretic tolerance, which can be a problem with long-term use of loop diuretics.
  • compounds that are ROMK inhibitors can be identified as those compounds which, when tested, have an IC50 of 5 ⁇ or less, preferably 1 ⁇ or less, and more preferably
  • the dosage amount of the compound to be administered depends on the individual case and is, as is customary, to be adapted to the individual circumstances to achieve an optimum effect. Thus, it depends on the nature and the severity of the disorder to be treated, and also on the sex, age, weight and individual responsiveness of the human or animal to be treated, on the efficacy and duration of action of the compounds used, on whether the therapy is acute or chronic or prophylactic, or on whether other active compounds are administered in addition to compounds of Formula I. A consideration of these factors is well within the purview of the ordinarily skilled clinician for the purpose of determining the therapeutically effective or prophylactically effective dosage amount needed to prevent, counter, or arrest the progress of the condition. It is expected that the compound will be administered chronically on a daily basis for a length of time appropriate to treat or prevent the medical condition relevant to the patient, including a course of therapy lasting days, months, years or the life of the patient.
  • a daily dose of approximately 0.001 to 100 mg/kg, preferably 0.001 to 30 mg/kg, in particular 0.001 to 10 mg/kg (in each case mg per kg of bodyweight) is appropriate for administration to an adult weighing approximately 75 kg in order to obtain the desired results.
  • the daily dose is preferably administered in a single dose or can be divided into several, for example two, three or four individual doses, and may be, for example but not limited to, 0.1 mg, 0.25 mg, 0.5 mg, 0.75 mg, 1 mg, 1.25 mg, 2 mg, 2.5 mg, 5 mg, 10 mg, 20 mg, 40 mg, 50 mg, 75 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, etc., on a daily basis. In some cases, depending on the individual response, it may be necessary to deviate upwards or downwards from the given daily dose.
  • the compound may be formulated for immediate or modified release such as extended or controlled release.
  • patient includes animals, preferably mammals and especially humans, who use the instant active agents for the prohylaxis or treatment of a medical condition.
  • Administering of the drug to the patient includes both self-administration and administration to the patient by another person.
  • the patient may be in need of treatment for an existing disease or medical condition, or may desire prophylactic treatment to prevent or reduce the risk for developing said disease or medical condition or developing long-term complications from a disease or medical condition.
  • therapeutically effective amount is intended to mean that amount of a drug or pharmaceutical agent that will elicit the biological or medical response of a tissue, a system, animal or human that is being sought by a researcher, veterinarian, medical doctor or other clinician.
  • a prophylactically effective amount is intended to mean that amount of a
  • a specific daily dosage amount can simultaneously be both a therapeutically effective amount, e.g., for treatment of hypertension, and a prophylactically effective amount, e.g., for prevention or reduction of risk of myocardial infarction or prevention and reduction of risk for complications related to hypertension.
  • the ROMK inhibitors may be administered via any suitable route of administration such as, for example, orally, parenterally, or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles.
  • parenteral as used herein includes subcutaneous injections, intravenous (IV), intramuscular, intrasternal injection or infusion techniques.
  • Oral formulations are preferred for treatment of chronic indications such as hypertension or chronic heart failure, particularly solid oral dosage units such as pills, tablets or capsules, and more particularly tablets. IN dosing is preferred for acute treatment, for example for the treatment of acute heart failure.
  • compositions comprised of a compound of Formula I and a pharmaceutically acceptable carrier which is comprised of one or more excipients or additives.
  • An excipient or additive is an inert substance used to formulate the active drug ingredient.
  • the pharmaceutical compositions of this invention containing the active ingredient may be in forms such as pills, tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsions, hard or soft capsules, or syrups or elixirs.
  • Compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions.
  • Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets.
  • the excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, mannitol, calcium phosphate or sodium
  • granulating and disintegrating agents for example, corn starch, or alginic acid
  • binding agents for example starch, gelatin or acacia
  • lubricating agents for example, magnesium stearate, stearic acid or talc.
  • compositions may also contain other customary additives, for example but not limited to, wetting agents, stabilizers, emulsifiers, dispersants, preservatives, sweeteners, colorants, flavorings, aromatizers, thickeners, buffer substances, solvents, solubilizers, agents for achieving a depot effect, salts for altering the osmotic pressure, coating agents or antioxidants.
  • customary additives for example but not limited to, wetting agents, stabilizers, emulsifiers, dispersants, preservatives, sweeteners, colorants, flavorings, aromatizers, thickeners, buffer substances, solvents, solubilizers, agents for achieving a depot effect, salts for altering the osmotic pressure, coating agents or antioxidants.
  • Oral immediate-release and time-controlled release dosage forms may be employed, as well as enterically coated oral dosage forms. Tablets may be uncoated or they may be coated by known techniques for aesthetic purposes, to mask taste or for other reasons. Coatings can also be used to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate may be employed.
  • Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredients is mixed with water or miscible solvents such as propylene glycol, PEGs and ethanol, or an oil medium, for example peanut oil, liquid paraffin, or olive oil.
  • an inert solid diluent for example, calcium carbonate, calcium phosphate or kaolin
  • water or miscible solvents such as propylene glycol, PEGs and ethanol
  • an oil medium for example peanut oil, liquid paraffin, or olive oil.
  • Aqueous suspensions contain the active material in admixture with excipients suitable for the manufacture of aqueous suspensions.
  • Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in mineral oil such as liquid paraffin.
  • the oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol.
  • Sweetening agents and flavoring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
  • Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose.
  • therapeutically effective amount of a compound of this invention can be used for the preparation of a medicament useful for inhibiting ROMK, for causing diuresis and/or natriuresis, and/or for treating, preventing or reducing the risk for any of the medical conditions described herein, in dosage amounts described herein.
  • the amount of active compound of Formula I and/or its pharmaceutically acceptable salts in the pharmaceutical composition may be, for example but not limited to, from 0.1 to 200 mg, particularly from 0.1 to 100 mg, and more particularly from 0.1 to 50 mg, per dose on a free acid/free base weight basis, but depending on the type of the pharmaceutical composition, potency of the active ingredient and/or the medical condition being treated, it could also be lower or higher.
  • Pharmaceutical compositions usually comprise 0.5 to 90 percent by weight of the active compound on a free acid/free base weight basis.
  • the compounds of Formula I inhibit ROMK. On account of this property, apart from use as pharmaceutically active compounds in human medicine and veterinary medicine, they can also be employed as a scientific tool or as aid for biochemical investigations in which such an effect on ROMK is intended, and also for diagnostic purposes, for example in the in vitro diagnosis of cell samples or tissue samples.
  • the compounds of Formula I can also be employed as
  • One or more additional pharmacologically active agents may be administered in combination with a compound of Formula I.
  • the additional active agent (or agents) is intended to mean a medicinal compound that is different from the compound of Formula I, and which is a pharmaceutically active agent (or agents) that is active in the body, including pro-drugs, for example esterified forms, that convert to pharmaceutically active form after administration, and also includes free-acid, free-base and pharmaceutically acceptable salts of said additional active agents when such forms are sold commercially or are otherwise chemically possible.
  • any suitable additional active agent or agents including but not limited to anti-hypertensive agents, additional diuretics, anti-atherosclerotic agents such as a lipid modifying compound, antidiabetic agents and/or anti-obesity agents may be used in any combination with the compound of Formula I in a single dosage formulation (a fixed dose drug combination), or may be
  • thiazide-like diuretics e.g., hydrochlorothiazide (HCTZ or HCT); angiotensin converting enzyme inhibitors (e.g, alacepril, benazepril, captopril, ceronapril, cilazapril, delapril, enalapril, enalaprilat, fosinopril, imidapril, lisinopril, moveltipril, perindopril, quinapril, ramipril, spirapril, temocapril, or trandolapril); dual inhibitors of angiotensin converting enzyme (ACE) and neutral endopeptidase (NEP) such as omapatrilat
  • ACE angiotensin converting enzyme
  • NEP neutral endopeptidase
  • eprosartan e.g., eprosartan mesylate (TEVETAN®), irbesartan (AVAPRO®), losartan, e.g., losartan potassium (COZAAR®), olmesartan, e.g, olmesartan medoximil
  • a thiazide-like diuretic such as hydrochlorothiazide (e.g., HYZAAR®, DIOVAN HCT®, ATACAND HCT®), etc.); potassium sparing diuretics such as amiloride HCl, spironolactone, epleranone, triamterene, each with or without HCTZ; carbonic anhydrase inhibitors, such as acetazolamide, neutral endopeptidase inhibitors (e.g., thiorphan and phosphoramidon); aldosterone antagonists; aldosterone synthase inhibitors; renin inhibitors (e.g.
  • urea derivatives of di- and tri-peptides See U.S. Pat. No. 5,116,835), amino acids and derivatives (U.S. Patents 5,095,119 and 5,104,869), amino acid chains linked by non-peptidic bonds (U.S. Patent 5,114,937), di- and tri-peptide derivatives (U.S. Patent 5,106,835), peptidyl amino diols (U.S. Patents 5,063,208 and 4,845,079) and peptidyl beta-aminoacyl aminodiol carbamates (U.S. Patent 5,089,471); also, a variety of other peptide analogs as disclosed in the following U.S.
  • Patent 5,066,643 enalkrein; RO 42-5892; A 65317; CP 80794; ES 1005; ES 8891; SQ 34017; aliskiren (2(S),4(S),5(S),7(S)-N-(2-carbamoyl-2-methylpropyl)-5-amino-4- hydroxy-2,7-diisopropyl-8-[4-methoxy-3-(3-methoxypropoxy)-phenyl]-octanamid hemifumarate) SPP600, SPP630 and SPP635); endothelin receptor antagonists; vasodilators (e.g.
  • calcium channel blockers e.g., amlodipine, nifedipine, verapamil, diltiazem, , felodipine, gallopamil, niludipine, nimodipine, nicardipine, bepridil, nisoldipine
  • potassium channel activators e.g., nicorandil, pinacidil, cromakalim, minoxidil, aprilkalim, loprazolam
  • sympatholitics e.g., acebutolol, atenolol, betaxolol, bisoprolol, carvedilol, metoprolol, metoprolol tartate, nadolol, propranolol, sotalol, timolol
  • beta-adrenergic blocking drugs e.g., acebutolol, atenolol, betaxolol, bisoprolol, carvedilol, metoprolol, metoprolol tartate, nadolol, propranolol, sotalol, timolol
  • alpha adrenergic blocking drugs e.g., doxazocin, prazocin or alpha methyldopa
  • central alpha adrenergic agonists e.g.
  • nitrates or nitric oxide donating compounds eg isosorbide mononitrate
  • lipid lowering agents e.g., HMG-CoA reductase inhibitors such as simvastatin and lovastatin which are marketed as ZOCOR® and MEVACOR® in lactone pro-drug form and function as inhibitors after administration, and pharmaceutically acceptable salts of dihydroxy open ring acid HMG-CoA reductase inhibitors such as atorvastatin (particularly the calcium salt sold in LIPITOR®), rosuvastatin (particularly the calcium salt sold in CRESTOR®), pravastatin (particularly the sodium salt sold in PRAVACHOL®), and fluvastatin (particularly the sodium salt sold in LESCOL®); a cholesterol absorption inhibitor such as ezetimibe (ZETIA®), and ezetimibe in combination with any other lipid lowering agents such as the HMG-CoA reductase inhibitors noted above and particularly with sim
  • TREDAPTIVE® and/or with an HMG-CoA reductase inhibitor
  • niacin in immediate-release or controlled release forms and particularly niacin in combination with a DP antagonist such as laropiprant (TREDAPTTVE®) and/or with an HMG-CoA reductase inhibitor
  • niacin receptor agonists such as acipimox and acifran, as well as niacin receptor partial agonists
  • metabolic altering agents including insulin sensitizing agents and related compounds for the treatment of diabetes such as biguanides (e.g., metformin), meglitinides (e.g., repaglinide, nateglinide), sulfonylureas (e.g., chlorpropamide, glimepiride, glipizide, glyburide, tolazamide, tolbutamide), thiazolidinediones also referred to as glitazones (e.g.,
  • the Boc group of 1-2 was then removed to afford amine 1-3, which was reacted with electrophile 1-4 or 1-5 to deliver compounds of formula II-l or II-2, respectively.
  • keto- piperazine 1-6 was first reacted with epoxide 1-7 to furnish carboxamide 1-8, which was further coupled to Z2- Halide under palladium catalysis conditions to provide compounds of formula II-2, as shown in Scheme 2.
  • the subject compounds may be prepared by modification of the procedures disclosed in the Examples as appropriate. Starting materials are commercially available or made by known procedures or as illustrated. The following examples are provided for the purpose of further illustration only and are not intended to be limitations on the disclosed invention.
  • reactions sensitive to moisture or air were performed under nitrogen or argon using anhydrous solvents and reagents.
  • the progress of reactions was determined by either analytical thin layer chromatography (TLC) usually performed with E. Merck precoated TLC plates, silica gel 60F-254, layer thickness 0.25 mm or liquid chromatography-mass spectrometry (LC-MS; also referred to as "LC” in the experimental procedures herein).
  • TLC analytical thin layer chromatography
  • LC-MS liquid chromatography-mass spectrometry
  • the analytical LC-MS system used consisted of a Waters ZQ platform with electrospray ionization in positive ion detection mode with an Agilent 1 100 series HPLC with autosampler.
  • the column was usually a
  • Preparative FfPLC purifications were usually performed using a mass spectrometry directed system. Usually they were performed on a Waters Chromatography Workstation configured with LC-MS System Consisting of: Waters ZQ single quad MS system with
  • Electrospray Ionization Waters 2525 Gradient Pump, Waters 2767 Injector / Collector, Waters 996 PDA Detetor, the MS Conditions of: 150-750 amu, Positive Electrospray, Collection Triggered by MS, and a Waters Sunfire C-l 8 5 micron, 30 mm (id) x 100 mm column.
  • the mobile phases consisted of mixtures of acetonitrile (10-100%) in water containing 0.1%TFA. Flow rates were maintained at 50 mL/min, the injection volume was 1800 ⁇ uL, and the UV detection range was 210-400 nm. Mobile phase gradients were optimized for the individual compounds.
  • Tetramethylsilane (TMS) was used as internal reference in CD 3 C1 solutions, and residual CH3OH peak or TMS was used as internal reference in CD3OD solutions. Coupling constants (J) were reported in hertz (Hz). Chiral analytical chromatography was performed on one of Chiralpak AS, Chiralpak AD, Chiralcel OD, Chiralcel IA, or Chiralcel OJ columns (250x4.6 mm) (Daicel Chemical Industries, Ltd.) with noted percentage of either ethanol in hexane (%Et/Hex) or isopropanol in heptane (%IP A/Hep) as isocratic solvent systems.
  • Chiral preparative chromatography was conducted on one of Chiralpak AS, Chiralpak AD, Chiralcel OD, Ciralcel IA, or Chiralcel OJ columns (20x250 mm) (Daicel Chemical Industries, Ltd.) with desired isocratic solvent systems identified on chiral analytical chromatography or by supercritical fluid chromatography (SFC) conditions.
  • Abbreviations used herein include: -C(0)CH 3 (Ac); acetic acid (AcOH); -OC(0)CH 3 (OAc); aqueous (aq); Cbz (benzyloxycarbonyl); N ⁇ V-diisopropylethylamine (DEEA); N;N- dimethylformamide (DMF); ethyl acetate (EtOAc; EA); diethyl ether (ether or Et 2 0); petroleum ether (Pet Ether; PE); 2-propanol (IPA); methyl t-butylether (MTBE); (benzotriazol-1- yloxy)tripyrrolidino-phosphonium hexafluorophosphate (PyBOP); saturated aq sodium chloride solution (brine); trifluoroacetic acid (TFA); tetrahydrofuran (THF); t-butyloxycarbonyl (Boc or BOC); Diethylaminosulfur tri
  • DCE dichloroethane
  • NPS N-iodosuccinamide
  • TfOH trifluoromethanesulfonic acid
  • NMP N-methyl morpholine
  • MsCl methanesulfonyl chloride
  • DVB 1,8- diazabicyclo[5.4.0]-undec-7-ene
  • TosMIC p-toulenesulfonylmethyl isocyanide
  • DME dimethoxyethane
  • TEDA tetramethyl ethylene diamine
  • DMAP 4-dimethylamino pyridine
  • Pd 2 dba 3 Tris(dibenzylideneacetone)dipalladium(O)); Xantphos (4,5-
  • Bis(diphenylphosphino)-9,9-dimethylxanthene gram(s) (g); milligram(s) (mg); microliter(s) ( ⁇ ); milliliter(s) (mL); millimole (mmol); minute(s) (min, mins); hour(s) (h, hr or hrs); retention time (R t ); room temperature (rt, r.t.
  • RT round bottom
  • RB thin layer chromatography
  • FC flash chromatography
  • LCMS or LC- MS liquid chromatography-mass spectrometry
  • SFC supercritical fluid chromatography
  • MPLC Medium Pressure Liquid Chromatography
  • HPLC High Pressure Liquid Chromatography
  • Celite is the tradename for diatomaceous earth filter aid.
  • Step A 5 -( 1 ,3 -Dioxolan-2-ylmethyl -2-benzofuran- 1 (3H)-one :
  • a three-neck 5L round bottomed flask equipped with a stir bar, firestone valve, thermocouple, condenser and heating mantle was charged with tri-t-butyl phosphonium tetrafluoroborate (500 mg, 1.72 mmol), palladium (II) acetate (250 mg, 1.1 mmol) and 5-bromo-2-benzofuran-l(3H)-one (100 g, 470 mmol).
  • DMF (1.88 L) was added to the flask, and the mixture was degassed three times by alternating vacuum and nitrogen purge.
  • bromo(l ,3-dioxolan-2-ylmethyl)zinc solution (1.03 L, 516 mmol) was added via canula and the mixture was again degassed three times. The mixture was then heated at 85 °C for 5 hours, checked by HPLC-MS, then stirred at 85 °C for 5 more hours. The mixture was then allowed to return to RT for overnight. 2-MethylTHF (2L) and brine were added, and the mixture was stirred for 5 min. The layers were separated and the aqueous layer was extracted again with 2-methylTHF. The organic layers were combined, washed three times with brine (4L each), dried over MgS04, filtered and concentrated.
  • Step B (1-Oxo-l J-dmvdro-2-benzofuran-5-vnacetaldehyde: 5-(L3-Dioxolan-2-ylmethyl -2- benzofuran-l(3H)-one (61 g, 280 mmol) was combined with water (2.2 L) in a 5 L round bottomed flask equipped with a Claisen adapter, thermocouple, stir bar and nitrogen bubbler. Aqueous HCl solution (2M, 1.14 L, 2.29 mol) was added and the resulting mixture was heated at 40 °C for 8 h. Then the mixture was stirred overnight at room temperature. The mixture was extracted three times with 2 L of ethyl acetate.
  • Step A 2-chloroquinoline-6-carbonitrile: To a flask containing a stir bar was added 6-bromo-2- chloroquinoline (0.90 g, 3.7 mmol), CuCN (0.50 g, 5.6 mmol) followed by addition of DMF (10 mL). The resulting mixture was then refluxed at 150 °C overnight. When the reaction appeared complete by LC analysis, the reaction flask was taken out of the oil bath and cooled to room temperature. To the reaction mixture was then poured DCM (20 mL) and a precipitate formed immediately, which was filtered, redissolved in DCM, absorbed onto silica gel, and purified by flash chromatography to provide the title product.
  • Step B tert-Butyl 4-(6-cvanoquinolin-2-yl ' )-3-oxopiperazine-l-carboxylate:
  • Step A 7-Methyl-2-oxo-l, 2-dihydroquinoline-6-carbonitrile
  • Step B 2-Chloro-7-methylquinoline-6-carbonitrile: A mixture of 7-Methyl-2-oxo-l, 2- dihydroquinoline-6-carbonitrile (10 g, 54 mmol) and POCl 3 (30 mL) was stirred at 110 °C for 2 h and then at 20 °C overnight. The mixture was carefully poured into a mixture of water and ice (400 mL) while being stirred vigorously. The product was collected by filtration and dried by co- evaporation with MeCN/Toluene to furnish the title product.
  • LC MS (IE, m/z): [M+l] + 203.
  • Step C tert-Butyl 4-(6-cvano-7-methylquinolin-2-yl)-3-oxopiperazine-l-carboxylate: To a 25 mL flask was added a stir bar, 2-Chloro-7-methylquinoline-6-carbonitrile (0.2 g, 0.98 mmol), Pd 2 dba 3 (0.11 g, 0.19 mmol), Xantphos (0.090 g, 0.090 mmol), Cesium Carbonate (0.26 g, 0.63 mmol) and tert-Butyl 3-oxopiperazine-l-carboxylate (0.29 g, 1.4 mmol). The resulting mixture was degassed and purged with N 2 three times.
  • Step A 3-Chloroquinoline-7-carbonitrile: To a flask containing a stir bar was added 7-Bromo-3- chloroquinoline (1.0 g, 4.1 mmol), CuCN (0.55 g, 6.2 mmol), and DMF (25 mL). The mixture was then refluxed at 150 °C overnight. When the reaction was complete, as evidenced by LC analysis, the reaction flask was taken out of the oil bath and cooled to room temperature. To the reaction mixture was then poured DCM (20 mL) and a precipitate formed immediately, which was filtered off. The crude product was adsorbed onto silica gel and purified by silica gel column chromatography (HexanesiDCM ⁇ 1 :1) to provide the title product.
  • Step B tert-Butyl 4-(7-cyanoquinolin-3-yl)-3-oxopiperazine-l-carboxylate
  • Step C 3-(2-Oxopiperazin-l-yl) quinoline-7-carbonitrile: tert-Butyl 4-(7-cyanoquinolin-3-yl)-3- oxopiperazine-l-carboxylate (0.030 g, 0.099 mmol) was treated with TFA (2 mL) and stirred for 20 minutes. Analysis of the reaction mixture by LC indicated that reaction had gone to completion. The solution was concentrated in vacuo and crude was azetroped with
  • Step A tert-Butyl 4-(6-bromo-2-naphthyl -3 -oxopiperazine-l-carboxylate: To a microwave tube charged with a stir bar was added 2,6-dibromonaphthalene (710 mg, 2.5 mmol), tert-butyl 3- oxopiperazine-l-carboxylate (250 mg, 1.2 mmol), copper(I) iodide (48 mg, 0.25 mmol), ⁇ , ⁇ '- dimethylcyclohexane-l,2-diamine (71 mg, 0.50 mmol), K 3 P0 4 (530 mg, 2.5 mmol), and dioxane (10 mL).
  • 2,6-dibromonaphthalene 710 mg, 2.5 mmol
  • tert-butyl 3- oxopiperazine-l-carboxylate 250 mg, 1.2 mmol
  • copper(I) iodide 48 mg, 0.25 m
  • Step B 6-(2-Oxopiperazin-l-yl)naphthalene-2-carbonitrile hydrochloride: To a flask charged with tert-butyl 4-(6-bromo-2-naphthyl)-3 -oxopiperazine-l-carboxylate (500mg, 1.2 mmol) and a stir bar was added copper(I) cyanide (220mg, 2.5 mmol) and DMF (20 mL). The mixture was heated to 135 °C for 12 hours under an atmosphere of N2. The reaction was diluted with EtOAc, washed with sat.
  • Step A 3-Chloroisoquinoline-7-carbonitrile: To a flask charged with 7-bromo-3- chloroisoquinoline (l.Og, 4.1 mmol) and a stir bar was added copper(I) cyanide (550mg, 6.2 mmol) and DMF (50 mL). The mixture was heated to 150 °C for 24 hours under an atmosphere of nitrogen. The reaction was diluted with EtOAc, washed with sat. NH 4 OAc and brine, dried over sodium sulfate, concentrated and purified by silica gel chromatography (Hexanes:DCM) and the solvent removed to afford the title compound. LC-MS (IE, m/z): 189 [M + 1] + .
  • Step B 3-(2-oxopiperazin-l-vnisoquinoline-7-carbonitrile hydrochloride
  • StepC 3-(2-oxopiperazin- 1 -yl)isoquinoline-7-carbonitrile:
  • the product of Step B was further treated with 4N HC1 in dioxane, and removal of solvent furnished 3-(2-oxopiperazin-l- yl)isoquinoline-7-carbonitrile hydrochloride.
  • the HC1 salt was washed with aqueous sodium carbonate, and back extracted with CHC1 3 -IPA (3:1) to furnish the title compound.
  • Step A tert-Butyl 4-(7-bromo-2-naphthvn-3-oxopiperazine-l-carboxylate: To a 20 mL microwave vial charged with 2,7-dibromonaphthalene (1.1 g, 3.8 mmol), tert-butyl 3- oxopiperazine-l-carboxylate (0.50 g, 2.5 mmol), and a stir bar was added copper (I) iodide (95 mg, 0.50 mmol), N,N'-dimethylcyclohexane-l,2-diamine (142 mg, 1.0 mmol), K 3 P0 4 (1 100 mg, 5.0 mmol), and dioxane (15 mL).
  • Step B 7-(2-oxopiperazin-l-yl ' )naphthalene-2-carbonitrile
  • the mixture was purged three times with nitrogen, and heated to 100 °C under N 2 for 16 hours.
  • the reaction was diluted with EtOAc, adsorbed onto silica gel, and purified by silica gel chromatography (Hexanes:EtOAc 1 : 1) to provide tert-butyl 4-(5-cyano-l, 3 -benzothiazol-2-yl)-3 -oxopiperazine-1 -carboxylate (95 mg, 35% yield).
  • the carboxylate was further treated with TFA to remove the Boc group.
  • Step A 7-chloroquinoline-3-carbonitrile: To a flask containing a stir bar was added 3-bromo-7- chloroquinoline (1.0 g, 4.1 mmol), CuCN (0.55 g, 6.2 mmol) followed by addition of DMF (25 mL). The resulting mixture was then heated to 150 °C overnight. When the reaction was complete as evidenced by LC analyisis, the reaction flask was taken out of the oil bath and cooled to RT. To the reaction mixture was then poured DCM (20 mL) and a precipitate formed immediately, which was filtered. The filtrated was washed with aq. NH4CI, dried over sodium sulfate, and purified by flash chromatography. LC-MS (IE, m/z) 189 [M + 1] + .
  • Step B tert-butyl 4-(3-cvanoquinolin-7-yl)-3-oxopiperazine-l-carboxylate
  • Step C 7-(2-oxopiperazin-l-yl)quinoline-3-carbonitrile: tert-Butyl 4-(3-cyanoquinolin-7-yl)-3- oxopiperazine-l-carboxylate was treated with TFA (2 mL) for 20 minutes at RT.
  • Step A 6-Chloro-2-iodothieno[Z3-b1pyridine: To a flask charged with 6-Chlorothieno[2,3- b]pyridine (500 mg, 3.0 mmol) and a stir bar was added NIS (800 mg, 3.5 mmol) and Triflic acid (5 mL). The mixture was monitored by TLC and allowed to stir at RT for 4 hours. The reaction was poured into ice, neutralized with aqueous sodium carbonate, extracted with DCM, dried over sodium sulfate, concentrated and purified by flash chromatography (Hex: EtOAc gradient) to obtain the title product. LC-MS (IE, tn/z): 296 [M + 1] + .
  • Step A 6-Bromo-2-(methylsulfanyl quinoline: A solution of 6-Bromo-2-chloroquinoline (0.68 g, 2.8 mmol) in DMF was treated with sodium thiomethoxide (0.22 g, 3.1 mmol). After 1 h at RT, the LC-MS indicated conversion. The solution was diluted with brine and extracted with EtOAc. The organic layer was removed, dried, filtered and concentrated. The crude product was used in the next step. LC-MS (IE, m/z): 256 [M + 1] + .
  • Step B 6-Bromo-2-(methylsulfonyl)quinoline:
  • Step A (3-Bromo-2-methylphenyl methanol: To a solution of 3-bromo-2-methyl benzoic acid (35 g, 163 mmol) in THF (200 mL) was added Borane THF Complex (1.0 M, 212 mL, 212 mmol). The mixture was allowed to stir for 24 h. The reaction was then quenched with water. The THF was removed under reduced pressure. The resulting solid was dissolved in ethyl acetate (500 mL), washed with IN HC1, sodium carbonate, and brine. The organic layer was dried over sodium sulfate and concentrated to afford (3-bromo-2-methylphenyl)methanol.
  • Step B 5-Bromo-4-methyl-2-benzofuran-l(3H)-one: To a flask charged with (3-bromo-2- methylphenyl)methanol (6.0 g, 30 mmol) was added a 1M TFA solution of Thallium
  • Trifluoroacetate (16.2 g, 29.8 mmol). The mixture was stirred at RT overnight. The solvent was removed under vacuum, and the residue was pumped under high vacuum for 30 min to ensure complete removal of TFA. To the residue was then added palladium(II) chloride (529 mg, 2.98 mmol), lithium chloride (2.53 g, 59.7 mmol), magnesium oxide (2.41 g, 59.7 mmol), and MeOH (150 mL). The reaction was flushed with CO twice, and kept under CO at RT. Analysis by LC showed a formation of product within 2 hours. To this solution was added ethyl acetate to precipitate the salts.
  • Step C 5-ethenyl-4-methyl-2-benzofuran-U3H -one: 5-Bromo-4-methyl-2-benzofuran-l(3H)- one (598 mg, 4.47 mmol), potassium vinyl trifluoroborate (507 mg, 2.23 mmmol), PdCl 2 (dppf)- CH 2 C1 2 Adduct (182 mg, 0.223 mmmol) , and TEA (0.622 mL, 4.47 mmol) were added to 10 mL ethanol in a 20 mL microwave tube.
  • Step D 4-methyl-5-oxiran-2-yl-2-benzofuran-l(3H)-one
  • Step E 1-15 A (slower eluting isomer and I-15B (faster eluting isomer):
  • Racemic 4-methyl-5-oxiran-2-yl-2-ben ⁇ ofuran-l(3H)-one was resolved on a ChiralPak® AD-H column (5x25cm) under SFC conditions on a Berger MGIII preparative SFC instrument.
  • the racemate was diluted to 50 mg/ml in 1 :1 DCM:MeOH. The separation was accomplished using 10% EtOH/C0 2 , flow rate 200 ml/min, 100 bar, 25 °C. 500 ul Injections were spaced every 2.12 mins. The faster eluting (2i?)-epoxide (I-15B) eluted at 5.2 min, and the slower eluting (25)- epoxide (I- 15 A) eluted at 5.6 min.
  • Step C 5-(2-hydroxyethyl -6-iodo-2-benzofuran-U3 /)-one
  • Step D 5-(2-hydroxyethyl -6-methyl-2-benzofuran-l(3H)-one: To a flask charged with 5-(2- hydroxyethyl)-6-iodo-2-benzofuran-l(3H)-one (6.00 g, 19.7 mmol) and a stir bar was added Pd 2 (dba) 3 (452 mg,0.493 mmol), PPh 3 (1 g, 4 mmol) and NMP (50 mL). The mixture was purged with N 2 and heated to 50 °C for 10 min, followed by addition of Cul (375 mg, 1.97 mmol).
  • Step F 5-e1henyl-6-methyl-2-benzofuran-l(3H)-one
  • Step G 6-methyl-5-oxiran-2-yl-2-benzofuran-l( ' 3H)-one
  • 6-Oxo-5,6J.8-tetrahvdronaphthalene-2-carbonitrile To a 20 mL microwave tube was added 5- bromo-2-tetralone (2.0 g, 8.9 mmol) and Palladium(O) Tetrakis (0.616 g, 0.533 mmol), zinc cyanide (1.0 g, 8.9 mmol), and DMF (8 mL). The tube was sealed, degassed and microwaved at 80 °C for 1.0 hour. TLC showed no starting material left. The mixture was diluted with ethyl acetate, washed with ammonium hydroxide (2M, 2 x), dried with sodium sulfate, filtered and evaporated under reduced pressure.
  • 5- bromo-2-tetralone 2.0 g, 8.9 mmol
  • Palladium(O) Tetrakis 0.616 g, 0.533 mmol
  • zinc cyanide 1.0 g, 8.9 mmol
  • DMF 8 mL
  • Step B Methyl 5-nitro-2-(2-oxoethyl)benzoate: To a solution of Methyl 5-nitro-2-(prop-2-en- 1 - yl)benzoate (0.20 g, 0.90 mmol) in water (2 mL) and THF (5 mL) was added osmium tetroxide (0.57 mL 2.5% solution, 0.045 mmol) and NMO (0.16 g, 1.4 mmol). The mixture was allowed to stir at RT for 16 hours and monitored by TLC. The reaction was diluted with EtOAc, washed with NH 4 C1 and brine, and concentrated. The residue was redissolved in MeOH and water.
  • Step A 5-Allyl-2-benzofuran-l(3H -one
  • a 4-neck, 22-L, round bottom flask equipped with a mechanical stirrer, thermocouple, nitrogen bubbler, and condenser was charged with 5-bromophthalide (650 g, 3.0 mol), allyltri-n-butyltin (1200 g, 3.6 mol), palladium tetrakis triphenylphosphine (100 g, 0.089 mol), lithium chloride (250 g, 5.9 mol) and toluene (8.8 L).
  • the mixture was evacuated and flushed with nitrogen 3 times and then was stirred at 100 °C for 4 hours. After slowly cooling to ambient temperature, the mixture was filtered and concentrated.
  • Step B 5-(2-HydroxyethylV2-berizofuran-l(3H)-one: 5-allyl-2-benzofuran-l(3H)-one (1.53 g, 8.78 mmol) was dissolved in methanol (30 mL). THF was added to solubilize the starting material. The resulting mixture was cooled in a dry ice acetone bath (-78 °C) and ozone was bubbled into the reaction until the color of the mixture changed to orange.
  • Step C 5-(2-Bromoetnyl -2-benzofuran-l( ' 3H)-one
  • Step A methyl 3-amino-2-chlorobenzoate: To a solution of methyl 2-chloro-3-nitrobenzoate (2.1 g, 9.7 mmol) in methanol (100 mL) and THF (20 mL) was added zinc powder (1.9 g, 29 mmol), ammonium formate (3.1 g, 49 mmol), and a few drops of acetic acid. The mixture was allowed to stir at RT for 18 hours. Most of the volatiles were removed under reduced pressure. The residue was redissolved in EtOAc (200 mL), washed with brine, concentrated and purified by MPLC to provide methyl 3-amino-2-chlorobenzoate.
  • Step B methyl 3-bromo-2-chlorobenzoate: To a solution of methyl 3-amino-2-chlorobenzoate (2.0 g, 11 mmol) in 48% HBr (10 mL) and water (20 mL) was added an aqueous solution of sodium nitrite (0.89 g, 13 mmol) at 0 °C. The mixture was allowed to stir at 0 °C for 30 minutes before it was added into a suspension of copper(I) bromide (2.3 g, 16 mmol) in water (10 mL) and 48% HBr (5 mL) at 0 °C. The reaction was allowed to warm to RT slowly, and then heated to 60 °C for 5 minutes.
  • Step C (3-bromo-2-chlorophenyl methanol: To a solution of methyl 3-bromo-2-chlorobenzoate (1.9 g, 7.6 mmol) in THF (30 mL) was added Super Hydride (23 mL, 23 mmol) at 0 °C. The reaction was allowed to stir for 16 hours. The reaction was diluted with EtOAc, washed with brine, dried over sodium sulfate, and purified by MPLC to provide (3-bromo-2- chlorophenyl)methanol. LC-MS (IE, m/z): 205 [M- 17] + ;
  • Step D 5-bromo-4-cMoro-2-benzofuran-l(3H -one: To a flask charged with (3-bromo-2- chlorophenyl)methanol (1.1 g, 4.8 mmol) and a stir bar was added thallium trifluoroacetate (2.9 g, 5.3 mmol) and TFA (6 mL). The mixture was allowed to stir at RT for 16 hours. The volatiles were removed under reduced pressure.
  • Step E 5-allyl-4-chloro-2-benzofuran-l(3H)-one: To a flask charged with 5-bromo-4-chloro-2- benzofuran-l(3H)-one (190 mg, 0.77 mmol) and a stir bar was added allyl tri-n-butyltin (0.36 mL, 1.2 mmol), PdCl 2 (dppf)-DCM complex, lithium chloride (0.098 mg, 2.3 mmol), and toluene (5 mL). The flask was fitted with a condensor, purged three times with nitrogen, and heated to reflux for 6 hours.
  • Step A 4-methyl-5-prop-2-en-l-yl-2-benzofuran-l(3H)-one:
  • Step B (4-methyl-l-oxo-l ,3-dihvdro-2-benzofuran-5-ynacetaldehvde
  • Step A 2-(Methyloxy -4-prop-2-en-l-ylbenzonitrile
  • Step A Methyl (3-fluoro-4-hvdroxyphenyl ' ) acetate
  • Step B Methyl (3-fluoro-4-( (trifluoromethyl ' )sulfonyl]oxy
  • the crude triflate (43.4 g) was subsequently dissolved in anhydrous dimethylformamide (100 mL).
  • Zinc cyanide (7.2 g, 78 mmol) was added, and the solution was purged thoroughly with nitrogen.
  • Tetrakis(triphenylphosphine) palladium(O) (12 g, 14 mmol) was then added and the reaction mixture was heated to 80 °C for 4h. After allowing cooling to ambient temperature and diluting with water (300 mL), ethyl acetate (500 mL) was added. The combined layers were filtered to remove any solids, the filtrate transferred to a separatory funnel, and the layers separated.
  • the aqueous layer was re-extracted with ethyl acetate (1 x 100 mL), the organic portions were combined and dried over magnesium sulfate. The dry organics were then filtered and evaporated to dryness under reduced pressure and excess dimethylformamide was removed by evaporation in vacuo at 65 °C for 1.5 h to yield the crude title compound (42 g).
  • 6- ⁇ 2- ⁇ ⁇ ⁇ -4-[2-( ⁇ - ⁇ ⁇ ⁇ -1 J-dihydro-2-benzofuran-5-yl)ethyl]piperazin-l-yl)naphthalene-2- carbonitrile To a 25 mL pear shaped flask charged with 6-(2-Oxopiperazin-l-yl)naphthalene-2- carbonitrile hydrochloride [1-5] (30 mg, 0.10 mmol) and a stir bar was added (l-Oxo-1,3- dihydro-2-benzofuran-5-yl)acetaldehyde [I-l](28 mg, 0.16 mmol), sodium triacetocyborohydride (22 mg, 0.10 mmol), DCM, and a few drops of acetic acid. The mixture was allowed to stir at RT for 16 hours. The title product was purified by mass-directed reverse phase HPLC (AcCN- Water with 0.1% TFA). LC-MS (IE, m/
  • Step A 2-Chloro-l,3-benzothiazole-6-carbonitrile is commercially available or can be prepared as described in Example 268 of US Patent Publication No. US2009/019862.
  • Step B A mixture of 4-[(2i?)-2-hydroxy-2-(4-methyl-l-oxo-l,3-dihydro-2-benzofuran-5- yl)ethyl]piperazin-2-one [I- 10] (30 mg, 0.10 mmol), 2-chloro-l,3-benzothiazole-6-carbonitrile (20 mg, 0.10 mmol), Pd 2 (dba) 3 (19 mg, 0.021 mmol), Xantphos (24 mg, 0.041 mmol), and Cs 2 C0 3 (50 mg, 0.16 mmol) in dioxane (2 mL) was sealed in a microwave tube and purged three times with nitrogen. It was heated to 120 °C for 10 minutes in a microwave reactor. LC showed formation of the title product, which was purified by silica gel flash chromatography. LC-MS (IE, m/z): 449 [M + 1] + .
  • a mixture of 4-[(2i?)-2-hydroxy-2-(4-methyl- 1 -oxo- 1 ,3- dihydro-2-benzofuran-5-yl)ethyl]piperazin-2-one 50 mg, 0.17 mmol
  • 6-Bromo-l- benzothiophene-2-carbonitrile [1-11](41 mg, 0.17 mmol), Pd 2 (dba) 3 (32 mg, 0.034 mmol), Xantphos (40 mg, 0.069 mmol), and Cs 2 C0 3 (84 mg, 0.26 mmol) in dioxane (1 mL) was sealed in
  • Thallium Flux Assay was performed on the final product compounds in all the Examples. This assay makes use of the ability of thallium to permeate through open ROMK channels and increase the fluorescence of a dye previously loaded into the cells. Under control conditions, cells loaded with dye and exposed to thallium-containing medium display a time- dependent increase in fluorescence, the rate of which depends on number of functional channels. When cells are incubated in the presence of a channel inhibitor, the increase in fluorescence is attenuated in a concentration-dependent manner, and IC50 values of inhibition by compounds can be accurately determined. This assay has been established with cell lines expressing either human, or rat ROMK channels, and operates in 384-well format.
  • HEK293 cells stably expressing hROMK (hK ir l.l) were grown at 37°C in a 10%CO 2 humidified incubator in complete growth media: Dulbecco's Modified Eagle Medium supplemented with non-essential amino acids, Penicillin/Streptomycin/Glutamine, G418 and FBS. At >80% confluency, aspirate the media from the flask and rinse with 10 mL
  • Probenecid (Component D) - Lyophilized sample is kept at -20°C. Water soluble, 100X after solubilization in 1 mL water. Store at 4°C.
  • Probenecid/ Assay Buffer 100 mL of IX FluxORTM Assay Buffer; 1 mL of reconstituted component D; Store at 4°C
  • Loading Buffer (per microplate): 10 ⁇ 1000X FluxORTM Reagent; 100 ⁇ component C; 10 mL Probenecid/ Assay Buffer
  • Compound Buffer (per microplate): 20 mL Probenecid/ Assay Buffer; 0.3 mM ouabain (10 mM ouabain in water can be stored in amber bottle/aluminum foil at room temperature); Test compound
  • Stimulant Buffer prepared at 5X final concentration in IX FluxORTMChloride-Free Buffer: 7.5 mM Thallium sulfate and 0.75 mM Potassium sulfate (to give a final assay concentration of 3 mM Thallium/ 0.3 mM Potassium). Store at 4°C when not in use. If kept sterile, this solution is good for months.
  • Assay protocol- The ROMK channel functional thallium flux assay is performed in 384 wells, using the FLIPR-Tetra instrument. HEK-hKirl.l cells are seeded in Poly-D-Lysine microplates and kept in a 37°C-10%CO 2 incubator overnight. On the day of the experiment, the growth media is replaced with the FluxORTM reagent loading buffer and incubated, protected from light, at ambient temperature (23-25°C) for 90 min. The loading buffer is replaced with assay buffer ⁇ test compound followed by 30 min incubation at ambient temperature, where the Thallium/Potassium stimulant is added to the microplate.
  • Step Protocol 1 Seed HEK-hKirl.l cells (50 ⁇ at 20,000 cells/well) in 384-well PDL coated Microplates
  • control compound is included to support that the assay is giving consistent results compared to previous measurements, although the control is not required to obtain the results for the test compounds.
  • the control can be any compound of Formula I of the present invention, preferably with an IC50 potency of less than 1 ⁇ in this assay.
  • the control could be another compound (outside the scope of Formula I) that has an IC50 potency in this assay of less than 1 ⁇ .

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Abstract

The present invention provides compounds of Formula I I and the pharmaceutically acceptable salts thereof, which are inhibitors of the ROMK (Kir1.1) channel. The compounds act as diuretics and natriuretics and are valuable pharmaceutically active compounds for the therapy and prophylaxis of medical conditions including cardiovascular diseases such as hypertension and conditions resulting from excessive salt and water retention.

Description

TITLE OF THE INVENTION
INHIBITORS OF THE RENAL OUTER MEDULLARY POTASSIUM CHANNEL.
BACKGROUND OF THE INVENTION
The Renal Outer Medullary Potassium (ROMK) channel (Kirl .1) (see e.g., Ho, K., et al.,
Cloning and expression of an inwardly rectifying ATP -regulated potassium channel, Nature, 1993, 362(6415): p. 31-8.1, 2; and Shuck, M.E., et al., Cloning and characterization of multiple forms of the human kidney ROM-K potassium channel, J Biol Chem, 1994, 269(39): p. 24261- 70) is a member of the inward rectifier family of potassium channels expressed in two regions of the kidney: thick ascending loop of Henle (TALH) and cortical collecting duct (CCD) (see
Hebert, S.C., et al., Molecular diversity and regulation of renal potassium channels, Physiol Rev, 2005, 85(1): p. 319-713). At the TALH, ROMK participates in potassium recycling across the luminal membrane which is critical for the function of the Na+/K+/2C1" co-transporter, the rate- determining step for salt reuptake in this part of the nephron. At the CCD, ROMK provides a pathway for potassium secretion that is tightly coupled to sodium uptake through the amiloride- sensitive sodium channel (see Reinalter, S.C., et al., Pharmacotyping of hypokalaemic salt-losing tubular disorders, Acta Physiol Scand, 2004, 181(4): p. 513-21; and Wang, W., Renal potassium channels: recent developments, Curr Opin Nephrol Hypertens, 2004, 13(5): p. 549-55). Selective inhibitors of the ROMK channel (also referred to herein as inhibitors of ROMK or ROMK inhibitors) are predicted to represent novel diuretics for the treatment of hypertension and other conditions where treatment with a diuretic would be beneficial with potentially reduced liabilities (i.e., hypo- or hyperkalemia, new onset of diabetes, dyslipidemia) over the currently used clinical agents (see Lifton, R.P., A.G. Gharavi, and D.S. Geller, Molecular mechanisms of human hypertension, Cell, 2001, 104(4): p. 545-56). Human genetics (Ji, W., et al., Rare independent mutations in renal salt handling genes contribute to blood pressure variation, Nat Genet, 2008, 40(5): p. 592-9; and Tobin, M.D., et al., Common variants in genes underlying monogenic hypertension and hypotension and blood pressure in the general population, Hypertension, 2008, 51(6): p. 1658-64) and genetic ablation of ROMK in rodents (see Lorenz, J.N., et al., Impaired renal NaCl absorption in mice lacking the ROMK potassium channel, a model for type II Banter's syndrome, J Biol Chem, 2002, 277(40): p. 37871-80 and Lu, M., et al., Absence of small conductance K+ channel (SK) activity in apical membranes of thick ascending limb and cortical collecting duct in ROMK (Bartter's) knockout mice, J Biol Chem, 2002, 277(40): p. 37881-7) support these expectations. To our knowledge, the first publicly disclosed small molecule selective inhibitors of ROMK, including VU590, were reported from work done at Vanderbilt University as described in Lewis, L.M., et al., High-Throughput Screening Reveals a Small-Molecule Inhibitor of the Renal Outer Medullary Potassium Channel and Kir 7.1, Mol Pharmacol, 2009, 76(5): p. 1094-1103. The compound VU591 was later reported in Bhave, G. et al., Development of a Selective Small-Molecule Inhibitor of Kirl. l,the Renal Outer Medullary Potassium Channel, Mol Pharmacol, 2011, 79(1), p.42-50, the text of which states that "ROMK (Kir 1.1), is a putative drug target for a novel class of loop diuretics that would lower blood pressure without causing hypokalemia."
Figure imgf000003_0001
Patent application publication number WO2010/129379, published November 11, 2010 having common representative Merck Sharp & Dohme Corp., (also published as
US2010/0286123 on same date), describes ROMK inhibitors having the generic formula:
Figure imgf000003_0002
and, e.g., an embodiment wherein
R5 and R6 are independently -H, -Ci-6 alkyl, -C3-6 cycloalkyl, -CF3, -CHF2, -C¾F or
-CH2OH; X is -H, -OH,-OCi-3alkyl, -F, oxo, NH2 or-CH3; and Xl is -H or -CH3.
Patent application publication number WO2012/058134, published May 3, 2012, having common representative Merck Sharp & Dohme Corp., describes ROMK inhibitors having the generic formula:
Figure imgf000003_0003
wherein A and B are mono and/or bicyclic aromatic groups; R2 is -H,
-Cl-6 alkyl, -C3.6 cycloalkyl, CF3, -CH2OH, or -CO2R, or R2 can be joined to Rl or RlOa to form a ring; R3 is -H, -Ci-6 alkyl, -C3.6 cycloalkyl, -OH, -F, -OC1.3 alkyl, or -CH2OH, or R3 can be joined to R 10b to form a ring.
Patent application publication number WO2012/058116, published May 3, 2012, having common representative Merck Sharp & Dohme Corp., describes ROMK inhibitors having the generic formula:
Figure imgf000003_0004
wherein R5 and R6 are independently -H, -Ci-6 alkyl or -C(0)OCi-3alkyl; and X, X1, Y and Y1 are independently -H or-Ci-6alkyl; or Yl can be joined together with Z2 to form a fused ring system.
However, continuing discovery of selective small molecule inhibitors of ROMK is still needed for the development of new treatments for hypertension and related disorders. The compounds of Formula I and salts thereof of this invention are selective inhibitors of the ROMK channel and could be used for the treatment of hypertension, heart failure and other conditions where treatment with a diuretic or natriuretic would be beneficial.
SUMMARY OF THE INVENTION
The present invention provides compounds of Formula I, defined herein, and the pharmaceutically acceptable salts thereof. The compounds of Formula I are inhibitors of the ROMK (Kirl.l) channel. As a result, the compounds of Formula I could be used in methods of treatment, inhibition or amelioration of one or more disease states that could benefit from inhibition of ROMK. The compounds of this invention could be used in methods of treatment which comprise administering a therapeutically or prophylactically effective amount of a compound of Formula I to a patient in need of a diuretic and/or natriuretic agent. Therefore, the compounds of Formula I could be valuable pharmaceutically active compounds for the therapy, prophylaxis or both of medical conditions, including, but not limited to, cardiovascular diseases such as hypertension and heart failure, and conditions associated with excessive salt and water retention. The compounds of this invention could further be used in combination with other therapeutically effective agents, including but not limited to, other drugs which are useful for the treatment of hypertension, heart failure and conditions associated withexcessive salt and water retention. The invention furthermore relates to processes for preparing compounds of Formula I, and pharmaceutical compositions which comprise compounds of Formula I. These and other aspects of the invention will be evident from the description contained herein.
DETAILED DESCRIPTION OF THE INVENTION
The present invention is directed to compounds having structural Formula I:
Figure imgf000004_0001
and the pharmaceutically acceptable salts thereof wherein: Zl i IS
Figure imgf000005_0001
wherein the dashed bonds represent the optional presence of -Ci_2alkanediyl- which, when present,
;
Figure imgf000005_0002
Rl is -H, -CH3, -CF3, -CHF2, -CH2F or -CH2OH;
R2 is -H, oxo (=0) or -Ci-6alkyl;
R4 is -H, -OH, oxo or -C l -6alkyl;
provided that when R4 is -OH or oxo, then R2 is not oxo;
R3 and R5 are each independently -H or -Ci_6alkyl; provided that Its is absent when R2 is oxo, and R5 is absent when R is oxo;
Rla is -H, halo or -Ci_3alkyl;
Rlb is -H, halo, -Ci-3alkyl, -O-Ci alkyl or -COOCi-3alkyl;
one of R2a and R2b 1S -CN and the other is -H or -Ci-3alkyl;
one of R2c and R2d 1S -S02-Ci-3alkyl and the other is -H or -Ci-3alkyl;
Xl, X , χ3 and X4 are each independently CH or N, provided that only one of χΐ, χ2, χ3 or χ is N;
X5, χ6 and X7 are each independently CH or N, provided that only one of X5, χ6 0r X7 is N; and
Ra and Rb are each independently -H, halo, -Ci_3alkyl, -0-Ci-3alkyl or -COOCi-3alkyl.
The present invention is further directed to compounds of Formula I having structural Formula Π:
Figure imgf000006_0001
In another embodiment of this invention are compounds of Formula I or Π wherein Zl is zl-a. In a class thereof are compounds wherein R2, R3 and R5 are each -H, and R4 is -H, -OH or oxo, and more particularly R4 is -H or -OH. In a sub-class are compounds wherein Rl is -H.
In another embodiment of this invention are compounds of Formula I or II wherein Zl is zl-b. In a class thereof are compounds wherein R2, R3 and R5 are each -H, and R4 is -H, -OH or oxo, and more particularly R4 is -H or -OH. In a sub-class are compounds wherein Rl is -H.
In another embodiment of this invention are compounds of Formula I or II wherein Zl is zl-c. In a class thereof are compounds wherein the dashed bonds in zl-c represent -Ci_
2alkanediyl- and z 1 -c has the formula z 1 -c 1 :
Figure imgf000006_0002
. In another class thereof are compounds wherein the dashed bonds in zl-c are absent and therefore -Ci_2alkanediyl- is not present, and zl-c has the formula zl-c2:
Figure imgf000006_0003
. In a sub-class of both classes are compounds wherein Rl is -H.
All structural Formulas and embodiments described herein include the pharmaceutically acceptable salts of the compounds defined therein.
As used herein except if noted otherwise, "alkyl" is intended to include both branched- and straight-chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms. Commonly used abbreviations for alkyl groups are used throughout the specification. For example the term "Ci-6 alkyl" (or "C1-C6 alkyl"), means linear or branched chain alkyl groups, including all isomers, having the specified number of carbon atoms and includes all of the hexyl and pentyl isomers as well as n-, iso-, sec- and tert-butyl (butyl, s-butyl, /-butyl, t-butyl;
Bu = butyl), n- and /-propyl (Pr = propyl), ethyl (Et) and methyl (Me). Halo means -F, -CI, -Br and -I. In the Formula I, preferred halos are -F and -CI.
As used herein "alkanediyl" is a saturated aliphatic divalent hydrocarbon group having the specified number of carbon atoms. Examples of alkanediyl groups include, but are not limited to, methanediyl, ethanediyl, propanediyl, butanediyl and the like. The term
-Ci_2alkanediyl- describes methanediyl or ethanediyl. Unless expressly depicted or described otherwise, variables depicted in a structural formula with a "floating" bond, such as Rl, are permitted on any available carbon atom in the ring to which the variable is attached.
The present invention encompasses all stereoisomeric forms of the compounds of Formula I. Centers of asymmetry that are present in the compounds of Formula I can all independently of one another have (R) or (S) configuration. When bonds to the chiral carbon are depicted as straight lines in the structural Formulas of the invention, or when a compound name is recited without a chiral designation for a chiral carbon, it is understood that both the (R) and (S) configurations of the chiral carbon and hence both enantiomers and mixtures thereof, are embraced within the Formula or by the name. The production of specific stereoisomers or mixtures thereof may be identified in the Examples where such stereoisomers or mixtures were obtained, but this in no way limits the inclusion of all stereoisomers and mixtures thereof from being within the scope of this invention.
The invention includes all possible enantiomers and diastereomers and mixtures of two or more stereoisomers, for example mixtures of enantiomers and/or diastereomers, in all ratios. Thus, enantiomers are a subject of the invention in enantiomerically pure form, both as levorotatory and as dextrorotatory antipodes, in the form of racemates and in the form of mixtures of the two enantiomers in all ratios. In the case of a cis/trans isomerism the invention includes both the cis form and the trans form as well as mixtures of these forms in all ratios. The preparation of individual stereoisomers can be carried out, if desired, by separation of a mixture by customary methods, for example by chromatography or crystallization, by the use of stereochemically uniform starting materials for the synthesis or by stereoselective synthesis. Optionally a derivatization can be carried out before a separation of stereoisomers. The separation of a mixture of stereoisomers can be carried out at an intermediate step during the synthesis of a compound of Formula I or it can be done on a final racemic product. Absolute stereochemistry may be determined by X-ray crystallography of crystalline products or crystalline intermediates which are derivatized, if necessary, with a reagent containing a stereogenic center of known configuration. Where compounds of this invention are capable of tautomerization, all individual tautomers as well as mixtures thereof are included in the scope of this invention. The present invention includes all such isomers, as well as salts, solvates (which includes hydrates) and solvated salts of such racemates, enantiomers, diastereomers and tautomers and mixtures thereof.
Reference to the compounds of Formula I herein encompasses the compounds of Formula II and all embodiments thereof. Reference to the compounds of this invention as those of a specific formula or embodiment or any other generic structural formula or specific compound described or claimed herein, is intended to encompass the specific compound or compounds falling within the scope of the Formula or embodiment, including salts thereof, particularly pharmaceutically acceptable salts, solvates (including hydrates) of such compounds and solvated (or hydrated) salt forms thereof, where such forms are possible unless specified otherwise.
In the compounds of Formula I, the atoms may exhibit their natural isotopic abundances, or one or more of the atoms may be artificially enriched in a particular isotope having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number predominantly found in nature. The present invention is meant to include all suitable isotopic variations of the compounds of Formula I. For example, different isotopic forms of hydrogen (H) include protium (lH) and deuterium (2H). Protium is the predominant hydrogen isotope found in nature. Enriching for deuterium may afford certain therapeutic advantages, such as increasing in vivo half-life or reducing dosage requirements, or may provide a compound useful as a standard for characterization of biological samples. Isotopically-enriched compounds within Formula I can be prepared without undue experimentation by conventional techniques well known to those skilled in the art or by processes analogous to those described in the Schemes and Examples herein using appropriate isotopically-enriched reagents and/or intermediates.
When the compounds of Formula I contain one or more acidic or basic groups the invention also includes the corresponding pharmaceutically acceptable salts. Thus, the compounds of Formula I which contain acidic groups can be used according to the invention, for example, as alkali metal salts, alkaline earth metal salts or as ammonium salts. Examples of such salts include but are not limited to sodium salts, potassium salts, calcium salts, magnesium salts or salts with ammonia or organic amines such as, for example, ethylamine, ethanolamine, triethanolamine or amino acids. Compounds of Formula I which contain one or more basic groups, i.e. groups which can be protonated, can be used according to the invention in the form of their acid addition salts with inorganic or organic acids as, for example but not limited to, salts with hydrogen chloride, hydrogen bromide, phosphoric acid, sulfuric acid, nitric acid, benzenesulfonic acid, methanesulfonic acid, p-toluenesulfonic acid, naphthalenedisulfonic acids, oxalic acid, acetic acid, trifluoroacetic acid, tartaric acid, lactic acid, salicylic acid, benzoic acid, formic acid, propionic acid, pivalic acid, diethylacetic acid, malonic acid, succinic acid, pimelic acid, fumaric acid, maleic acid, malic acid, sulfaminic acid, phenylpropionic acid, gluconic acid, ascorbic acid, isonicotinic acid, citric acid, adipic acid, etc. If the compounds of Formula I simultaneously contain acidic and basic groups in the molecule the invention also includes, in addition to the salt forms mentioned, inner salts or betaines (zwitterions). Salts can be obtained from the compounds of Formula I by customary methods which are known to the person skilled in the art, for example by combination with an organic or inorganic acid or base in a solvent or dispersant, or by anion exchange or cation exchange from other salts. The present invention also includes all salts of the compounds of Formula I which, owing to low physiological
compatibility, are not directly suitable for use in pharmaceuticals but which can be used, for example, as intermediates for chemical reactions or for the preparation of pharmaceutically acceptable salts.
Furthermore, compounds of the present invention may exist in amorphous form and/or one or more crystalline forms, and as such all amorphous and crystalline forms and mixtures thereof of the compounds of Formula I are intended to be included within the scope of the present invention. In addition, some of the compounds of the instant invention may form solvates with water (i.e., a hydrate) or common organic solvents. Such solvates and hydrates, particularly the pharmaceutically acceptable solvates and hydrates, of the instant compounds are likewise encompassed within the scope of this invention, along with un-solvated and anhydrous forms.
Any pharmaceutically acceptable pro-drug modification of a compound of this invention which results in conversion in vivo to a compound within the scope of this invention is also within the scope of this invention. For example, esters can optionally be made by esterification of an available carboxylic acid group or by formation of an ester on an available hydroxy group in a compound. Similarly, labile amides can be made. Pharmaceutically acceptable esters or amides of the compounds of this invention may be prepared to act as pro-drugs which can be hydrolyzed back to an acid (or -COO- depending on the pH of the fluid or tissue where conversion takes place) or hydroxy form particularly in vivo and as such are encompassed within the scope of this invention. Examples of pharmaceutically acceptable pro-drug modifications include, but are not limited to, -Ci -6alkyl esters and -C i -6alkyl substituted with phenyl esters.
Accordingly, the compounds within the generic structural formulas, embodiments and specific compounds described and claimed herein encompass salts, all possible stereoisomers and tautomers, physical forms (e.g., amorphous and crystalline forms), solvate and hydrate forms thereof and any combination of these forms, as well as the salts thereof, pro-drug forms thereof, and salts of pro-drug forms thereof, where such forms are possible unless specified otherwise.
The compounds of Formula I according to the invention are inhibitors of ROMK, and therefore could be used as diuretic and/or natriuretic agents. ROMK inhibitors may be used to help to increase urination and increase urine volume and also to prevent or reduce reabsorption of sodium in the kidneys leading to increased excretion of sodium and water. Therefore, the compounds could be used for treatment or prophylaxis or both of disorders that benefit from increased excretion of water and sodium from the body. Accordingly, the compounds of this invention could be used in a method for inhibiting ROMK comprising administering a compound of Formula I in a ROMK-inhibitory effective amount to a patient in need thereof. The inhibition of ROMK by the compounds of Formula I can be examined, for example, in the Thallium Flux Assay described below. Moreover, this invention also relates to the use of the compounds of Formula I or salts thereof to validate in vitro assays, for example but not limited to the Thallium Flux Assay described herein. The compounds of this invention could be used in a method for causing diuresis, natriuresis or both, comprising administering a compound of Formula I in a therapeutically effective amount to a patient in need thereof. Therefore, the compounds of Formual I of this invention could be used in methods for treatment of, prevention of or reduction of risk for developing medical conditions that benefit from increased excretion of water and sodium, such as but not limited to one or more of hypertension, heart failure (both acute and chronic, the latter also known as congestive heart failure) and/or other conditions associated with excessive salt and water retention. Furthermore, the compounds of Formula I could be used in methods for treatment of, prevention of or reduction of risk for developing one or more disorders such as pulmonary arterial hypertension (PAH), cardiovascular disease, diabetes mellitus, diabetes insipidus, post-operative volume overload, endothelial dysfunction, diastolic dysfunction, systolic dysfunction, stable and unstable angina pectoris, thromboses, restenosis, myocardial infarction, stroke, cardiac insufficiency, pulmonary hypertonia, atherosclerosis, hepatic cirrhosis, ascitis, pre-eclampsia, cerebral edema, nephropathy, glomerulonephritis, nephrotic syndrome, acute and chronic kidney insufficiency (also referred to as chronic kidney disease, or more generally as renal impairment), acute tubular necrosis, hypercalcemia, idiopathic edema, Dent's disease, Meniere's disease, edematous states, glaucoma, benign intracranial hypertension, and other conditions for which a diuretic or natriuretic or both would have therapeutic or prophylactic benefit. The compounds of the invention may be administered to a patient having, or at risk of having, one or more conditions for which a diuretic or natriuretic or both would have therapeutic or prophylactic benefit such as those described herein.
The compounds of Formula I may potentially have reduced liabilities (for example, hypo- or hyperkalemia, new onset of diabetes, dyslipidemia, etc.) over currently used clinical agents. Also the compounds may have reduced risk for diuretic tolerance, which can be a problem with long-term use of loop diuretics.
In general, compounds that are ROMK inhibitors can be identified as those compounds which, when tested, have an IC50 of 5 μΜ or less, preferably 1 μΜ or less, and more preferably
0.25 μΜ or less, in the Thallium Flux Assay, which is described in more detail further below.
The dosage amount of the compound to be administered depends on the individual case and is, as is customary, to be adapted to the individual circumstances to achieve an optimum effect. Thus, it depends on the nature and the severity of the disorder to be treated, and also on the sex, age, weight and individual responsiveness of the human or animal to be treated, on the efficacy and duration of action of the compounds used, on whether the therapy is acute or chronic or prophylactic, or on whether other active compounds are administered in addition to compounds of Formula I. A consideration of these factors is well within the purview of the ordinarily skilled clinician for the purpose of determining the therapeutically effective or prophylactically effective dosage amount needed to prevent, counter, or arrest the progress of the condition. It is expected that the compound will be administered chronically on a daily basis for a length of time appropriate to treat or prevent the medical condition relevant to the patient, including a course of therapy lasting days, months, years or the life of the patient.
In general, a daily dose of approximately 0.001 to 100 mg/kg, preferably 0.001 to 30 mg/kg, in particular 0.001 to 10 mg/kg (in each case mg per kg of bodyweight) is appropriate for administration to an adult weighing approximately 75 kg in order to obtain the desired results. The daily dose is preferably administered in a single dose or can be divided into several, for example two, three or four individual doses, and may be, for example but not limited to, 0.1 mg, 0.25 mg, 0.5 mg, 0.75 mg, 1 mg, 1.25 mg, 2 mg, 2.5 mg, 5 mg, 10 mg, 20 mg, 40 mg, 50 mg, 75 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, etc., on a daily basis. In some cases, depending on the individual response, it may be necessary to deviate upwards or downwards from the given daily dose. Furthermore, the compound may be formulated for immediate or modified release such as extended or controlled release.
The term "patient" includes animals, preferably mammals and especially humans, who use the instant active agents for the prohylaxis or treatment of a medical condition.
Administering of the drug to the patient includes both self-administration and administration to the patient by another person. The patient may be in need of treatment for an existing disease or medical condition, or may desire prophylactic treatment to prevent or reduce the risk for developing said disease or medical condition or developing long-term complications from a disease or medical condition.
The term therapeutically effective amount is intended to mean that amount of a drug or pharmaceutical agent that will elicit the biological or medical response of a tissue, a system, animal or human that is being sought by a researcher, veterinarian, medical doctor or other clinician. A prophylactically effective amount is intended to mean that amount of a
pharmaceutical drug that will prevent or reduce the risk of occurrence of the biological or medical event that is sought to be prevented in a tissue, a system, animal or human by a researcher, veterinarian, medical doctor or other clinician. It is understood that a specific daily dosage amount can simultaneously be both a therapeutically effective amount, e.g., for treatment of hypertension, and a prophylactically effective amount, e.g., for prevention or reduction of risk of myocardial infarction or prevention and reduction of risk for complications related to hypertension.
In the methods of treatment of this invention, the ROMK inhibitors may be administered via any suitable route of administration such as, for example, orally, parenterally, or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles. The term parenteral as used herein includes subcutaneous injections, intravenous (IV), intramuscular, intrasternal injection or infusion techniques. Oral formulations are preferred for treatment of chronic indications such as hypertension or chronic heart failure, particularly solid oral dosage units such as pills, tablets or capsules, and more particularly tablets. IN dosing is preferred for acute treatment, for example for the treatment of acute heart failure.
This invention also provides pharmaceutical compositions comprised of a compound of Formula I and a pharmaceutically acceptable carrier which is comprised of one or more excipients or additives. An excipient or additive is an inert substance used to formulate the active drug ingredient. For oral use, the pharmaceutical compositions of this invention containing the active ingredient may be in forms such as pills, tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsions, hard or soft capsules, or syrups or elixirs. Compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions. Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets. The excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, mannitol, calcium phosphate or sodium
phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid;
binding agents, for example starch, gelatin or acacia, and lubricating agents, for example, magnesium stearate, stearic acid or talc.
Pharmaceutical compositions may also contain other customary additives, for example but not limited to, wetting agents, stabilizers, emulsifiers, dispersants, preservatives, sweeteners, colorants, flavorings, aromatizers, thickeners, buffer substances, solvents, solubilizers, agents for achieving a depot effect, salts for altering the osmotic pressure, coating agents or antioxidants.
Oral immediate-release and time-controlled release dosage forms may be employed, as well as enterically coated oral dosage forms. Tablets may be uncoated or they may be coated by known techniques for aesthetic purposes, to mask taste or for other reasons. Coatings can also be used to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate may be employed.
Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredients is mixed with water or miscible solvents such as propylene glycol, PEGs and ethanol, or an oil medium, for example peanut oil, liquid paraffin, or olive oil.
Aqueous suspensions contain the active material in admixture with excipients suitable for the manufacture of aqueous suspensions. Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents and flavoring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid. Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose.
The instant invention also encompasses a process for preparing a pharmaceutical composition comprising combining a compound of Formula I with a pharmaceutically acceptable carrier. Also encompassed is the pharmaceutical composition which is made by combining a compound of Formula I with a pharmaceutically acceptable carrier. Furthermore, a
therapeutically effective amount of a compound of this invention can be used for the preparation of a medicament useful for inhibiting ROMK, for causing diuresis and/or natriuresis, and/or for treating, preventing or reducing the risk for any of the medical conditions described herein, in dosage amounts described herein.
The amount of active compound of Formula I and/or its pharmaceutically acceptable salts in the pharmaceutical composition may be, for example but not limited to, from 0.1 to 200 mg, particularly from 0.1 to 100 mg, and more particularly from 0.1 to 50 mg, per dose on a free acid/free base weight basis, but depending on the type of the pharmaceutical composition, potency of the active ingredient and/or the medical condition being treated, it could also be lower or higher. Pharmaceutical compositions usually comprise 0.5 to 90 percent by weight of the active compound on a free acid/free base weight basis.
The compounds of Formula I inhibit ROMK. On account of this property, apart from use as pharmaceutically active compounds in human medicine and veterinary medicine, they can also be employed as a scientific tool or as aid for biochemical investigations in which such an effect on ROMK is intended, and also for diagnostic purposes, for example in the in vitro diagnosis of cell samples or tissue samples. The compounds of Formula I can also be employed as
intermediates for the preparation of other pharmaceutically active compounds.
One or more additional pharmacologically active agents may be administered in combination with a compound of Formula I. The additional active agent (or agents) is intended to mean a medicinal compound that is different from the compound of Formula I, and which is a pharmaceutically active agent (or agents) that is active in the body, including pro-drugs, for example esterified forms, that convert to pharmaceutically active form after administration, and also includes free-acid, free-base and pharmaceutically acceptable salts of said additional active agents when such forms are sold commercially or are otherwise chemically possible. Generally, any suitable additional active agent or agents, including but not limited to anti-hypertensive agents, additional diuretics, anti-atherosclerotic agents such as a lipid modifying compound, antidiabetic agents and/or anti-obesity agents may be used in any combination with the compound of Formula I in a single dosage formulation (a fixed dose drug combination), or may be
administered to the patient in one or more separate dosage formulations which allows for concurrent or sequential administration of the active agents (co-administration of the separate active agents). Examples of the one or more additional active agents which may be employed include but are not limited to thiazide-like diuretics, e.g., hydrochlorothiazide (HCTZ or HCT); angiotensin converting enzyme inhibitors (e.g, alacepril, benazepril, captopril, ceronapril, cilazapril, delapril, enalapril, enalaprilat, fosinopril, imidapril, lisinopril, moveltipril, perindopril, quinapril, ramipril, spirapril, temocapril, or trandolapril); dual inhibitors of angiotensin converting enzyme (ACE) and neutral endopeptidase (NEP) such as omapatrilat, sampatrilat and fasidotril; angiotensin II receptor antagonists, also known as angiotensin receptor blockers or ARBs, which may be in free-base, free-acid, salt or pro-drug form, such as azilsartan, e.g., azilsartan medoxomil potassium (EDARBI®), candesartan, e.g., candesartan cilexetil
(ATACAND®), eprosartan, e.g., eprosartan mesylate (TEVETAN®), irbesartan (AVAPRO®), losartan, e.g., losartan potassium (COZAAR®), olmesartan, e.g, olmesartan medoximil
(BENICAR®), telmisartan (MICARDIS®), valsartan (DIOVAN®), and any of these drugs used in combination with a thiazide-like diuretic such as hydrochlorothiazide (e.g., HYZAAR®, DIOVAN HCT®, ATACAND HCT®), etc.); potassium sparing diuretics such as amiloride HCl, spironolactone, epleranone, triamterene, each with or without HCTZ; carbonic anhydrase inhibitors, such as acetazolamide, neutral endopeptidase inhibitors (e.g., thiorphan and phosphoramidon); aldosterone antagonists; aldosterone synthase inhibitors; renin inhibitors (e.g. urea derivatives of di- and tri-peptides (See U.S. Pat. No. 5,116,835), amino acids and derivatives (U.S. Patents 5,095,119 and 5,104,869), amino acid chains linked by non-peptidic bonds (U.S. Patent 5,114,937), di- and tri-peptide derivatives (U.S. Patent 5,106,835), peptidyl amino diols (U.S. Patents 5,063,208 and 4,845,079) and peptidyl beta-aminoacyl aminodiol carbamates (U.S. Patent 5,089,471); also, a variety of other peptide analogs as disclosed in the following U.S. Patents 5,071,837; 5,064,965; 5,063,207; 5,036,054; 5,036,053; 5,034,512 and 4,894,437, and small molecule renin inhibitors (including diol sulfonamides and sulfinyls (U.S. Patent 5,098,924), N-morpholino derivatives (U.S. Patent 5,055,466), N-heterocyclic alcohols (U.S. Patent 4,885,292) and pyrolimidazolones (U.S. Patent 5,075,451); also, pepstatin derivatives (U.S. Patent 4,980,283) and fluoro- and chloro-derivatives of statone-containing peptides (U.S. Patent 5,066,643); enalkrein; RO 42-5892; A 65317; CP 80794; ES 1005; ES 8891; SQ 34017; aliskiren (2(S),4(S),5(S),7(S)-N-(2-carbamoyl-2-methylpropyl)-5-amino-4- hydroxy-2,7-diisopropyl-8-[4-methoxy-3-(3-methoxypropoxy)-phenyl]-octanamid hemifumarate) SPP600, SPP630 and SPP635); endothelin receptor antagonists; vasodilators (e.g. nitroprusside); calcium channel blockers (e.g., amlodipine, nifedipine, verapamil, diltiazem, , felodipine, gallopamil, niludipine, nimodipine, nicardipine, bepridil, nisoldipine); potassium channel activators (e.g., nicorandil, pinacidil, cromakalim, minoxidil, aprilkalim, loprazolam);
sympatholitics; beta-adrenergic blocking drugs (e.g., acebutolol, atenolol, betaxolol, bisoprolol, carvedilol, metoprolol, metoprolol tartate, nadolol, propranolol, sotalol, timolol); alpha adrenergic blocking drugs (e.g., doxazocin, prazocin or alpha methyldopa); central alpha adrenergic agonists; peripheral vasodilators (e.g. hydralazine); nitrates or nitric oxide donating compounds eg isosorbide mononitrate, lipid lowering agents, e.g., HMG-CoA reductase inhibitors such as simvastatin and lovastatin which are marketed as ZOCOR® and MEVACOR® in lactone pro-drug form and function as inhibitors after administration, and pharmaceutically acceptable salts of dihydroxy open ring acid HMG-CoA reductase inhibitors such as atorvastatin (particularly the calcium salt sold in LIPITOR®), rosuvastatin (particularly the calcium salt sold in CRESTOR®), pravastatin (particularly the sodium salt sold in PRAVACHOL®), and fluvastatin (particularly the sodium salt sold in LESCOL®); a cholesterol absorption inhibitor such as ezetimibe (ZETIA®), and ezetimibe in combination with any other lipid lowering agents such as the HMG-CoA reductase inhibitors noted above and particularly with simvastatin (VYTORIN®) or with atorvastatin calcium; niacin in immediate-release or controlled release forms, and particularly niacin in combination with a DP antagonist such as laropiprant
(TREDAPTIVE®) and/or with an HMG-CoA reductase inhibitor; niacin in immediate-release or controlled release forms, and particularly niacin in combination with a DP antagonist such as laropiprant (TREDAPTTVE®) and/or with an HMG-CoA reductase inhibitor; niacin receptor agonists such as acipimox and acifran, as well as niacin receptor partial agonists; metabolic altering agents including insulin sensitizing agents and related compounds for the treatment of diabetes such as biguanides (e.g., metformin), meglitinides (e.g., repaglinide, nateglinide), sulfonylureas (e.g., chlorpropamide, glimepiride, glipizide, glyburide, tolazamide, tolbutamide), thiazolidinediones also referred to as glitazones (e.g., pioglitazone, rosiglitazone), alpha glucosidase inhibitors (e.g., acarbose, miglitol), dipeptidyl peptidase inhibitors, (e.g., sitagliptin (JANUVIA®), alogliptin, vildagliptin, saxagliptin, linagliptin, dutogliptin, gemigliptin), ergot alkaloids (e.g., bromocriptine), combination medications such as JANUMET® (sitagliptin with metformin), and injectable diabetes medications such as exenatide and pramlintide acetate; or with other drugs beneficial for the prevention or the treatment of the above-mentioned diseases including but not limited to diazoxide; and including the free-acid, free-base, and
pharmaceutically acceptable salt forms, pro-drug forms including but not limited to esters, and salts of pro-drugs of the above medicinal agents where chemically possible. Trademark names of pharmaceutical drugs noted above are provided for exemplification of the marketed form of the active agent(s); such pharmaceutical drugs could be used in a separate dosage form for concurrent or sequential administration with a compound of Formula I, or the active agent(s) therein could be used in a fixed dose drug combination including a compound of Formula I.
Several methods for preparing the compounds of this invention are described in the examples. Starting materials and intermediates are purchased, made from known procedures, or as otherwise illustrated. Some frequently applied routes to the compounds of Formula I are also described by the Schemes as follows. In some cases the order of carrying out the the steps of reaction schemes may be varied to facilitate the reaction or to avoid unwanted reaction products. The Ar group shown in the below schemes can represent any of the mono- or bi-cyclic rings at the terminal end of Zl as defined previously.
Two general methods were used for the synthesis of the compounds of Formula I. As shown In Scheme 1, N-Boc piperazine 1-1 may be coupled to Z2- Halide (Halide = CI, Br, or I) under palladium catalysis conditions (Buchwald, et. al. Organic Letters, 2000, 2(8), 1101-1104.). The Boc group of 1-2 was then removed to afford amine 1-3, which was reacted with electrophile 1-4 or 1-5 to deliver compounds of formula II-l or II-2, respectively. Alternatively, keto- piperazine 1-6 was first reacted with epoxide 1-7 to furnish carboxamide 1-8, which was further coupled to Z2- Halide under palladium catalysis conditions to provide compounds of formula II-2, as shown in Scheme 2.
SCHEME 1
Figure imgf000016_0001
The independent synthesis of diastereomers and enantiomers or their chromatographic separations may be achieved as known in the art by appropriate modification of the methodology disclosed herein. Their absolute stereochemistry may be determined by x-ray crystallography of crystalline products or crystalline intermediates which are derivatized, if necessary, with a reagent containing an asymmetric center of known absolute stereochemistry.
The subject compounds may be prepared by modification of the procedures disclosed in the Examples as appropriate. Starting materials are commercially available or made by known procedures or as illustrated. The following examples are provided for the purpose of further illustration only and are not intended to be limitations on the disclosed invention.
Reactions sensitive to moisture or air were performed under nitrogen or argon using anhydrous solvents and reagents. The progress of reactions was determined by either analytical thin layer chromatography (TLC) usually performed with E. Merck precoated TLC plates, silica gel 60F-254, layer thickness 0.25 mm or liquid chromatography-mass spectrometry (LC-MS; also referred to as "LC" in the experimental procedures herein). Typically the analytical LC-MS system used consisted of a Waters ZQ platform with electrospray ionization in positive ion detection mode with an Agilent 1 100 series HPLC with autosampler. The column was usually a
Water Xterra MS C 18, 3.0 χ 50 fexample 49 mm, 5 μτη. The flow rate was 1 mL/min, and the injection volume was 10 μΐ,. UV detection was in the range 210-400 nm. The mobile phase consisted of solvent A (water plus 0.06% TFA) and solvent B (acetonitrile plus 0.05% TFA) with a gradient of 100% solvent A for 0.7 min changing to 100% solvent B over 3.75 min, maintained for 1.1 min, then reverting to 100% solvent A over 0.2 min.
Preparative FfPLC purifications were usually performed using a mass spectrometry directed system. Usually they were performed on a Waters Chromatography Workstation configured with LC-MS System Consisting of: Waters ZQ single quad MS system with
Electrospray Ionization, Waters 2525 Gradient Pump, Waters 2767 Injector / Collector, Waters 996 PDA Detetor, the MS Conditions of: 150-750 amu, Positive Electrospray, Collection Triggered by MS, and a Waters Sunfire C-l 8 5 micron, 30 mm (id) x 100 mm column. The mobile phases consisted of mixtures of acetonitrile (10-100%) in water containing 0.1%TFA. Flow rates were maintained at 50 mL/min, the injection volume was 1800 ^uL, and the UV detection range was 210-400 nm. Mobile phase gradients were optimized for the individual compounds.
Reactions perfomed using microwave iradiation were normally carried out using an Emrys Optimizer manufactured by Personal Chemistry, or an Initiator manufactured by Biotage. Concentration of solutions was carried out on a rotary evaporator under reduced pressure. Flash chromatography was usually performed using a Biotage Flash Chromatography apparatus (Dyax Corp.) on silica gel (32-63 mM, 60 A pore size) in pre-packed cartridges of the size noted. 1H NMR spectra were acquired at 500 MHz spectrometers in CDC13 solutions unless otherwise noted. Chemical shifts were reported in parts per million (ppm). Tetramethylsilane (TMS) was used as internal reference in CD3C1 solutions, and residual CH3OH peak or TMS was used as internal reference in CD3OD solutions. Coupling constants (J) were reported in hertz (Hz). Chiral analytical chromatography was performed on one of Chiralpak AS, Chiralpak AD, Chiralcel OD, Chiralcel IA, or Chiralcel OJ columns (250x4.6 mm) (Daicel Chemical Industries, Ltd.) with noted percentage of either ethanol in hexane (%Et/Hex) or isopropanol in heptane (%IP A/Hep) as isocratic solvent systems. Chiral preparative chromatography was conducted on one of Chiralpak AS, Chiralpak AD, Chiralcel OD, Ciralcel IA, or Chiralcel OJ columns (20x250 mm) (Daicel Chemical Industries, Ltd.) with desired isocratic solvent systems identified on chiral analytical chromatography or by supercritical fluid chromatography (SFC) conditions.
In the Examples, when a compound is obtained via chromatography (e.g., MPLC, HPLC, silica gel), it means that the solvent was removed (generally under vacuum) after the
chromatography step to obtain the isolated product.
Abbreviations used herein include: -C(0)CH3 (Ac); acetic acid (AcOH); -OC(0)CH3 (OAc); aqueous (aq); Cbz (benzyloxycarbonyl); N^V-diisopropylethylamine (DEEA); N;N- dimethylformamide (DMF); ethyl acetate (EtOAc; EA); diethyl ether (ether or Et20); petroleum ether (Pet Ether; PE); 2-propanol (IPA); methyl t-butylether (MTBE); (benzotriazol-1- yloxy)tripyrrolidino-phosphonium hexafluorophosphate (PyBOP); saturated aq sodium chloride solution (brine); trifluoroacetic acid (TFA); tetrahydrofuran (THF); t-butyloxycarbonyl (Boc or BOC); Diethylaminosulfur trifluoride (DAST); dichloromethane (DCM); dimethylacetamide (DMA; DM AC); dimethylsulfoxide (DMSO); l,3-Bis(diphenylphosphino)propane (DPPP); acetic acid (HOAc); 3-chloroperoxybenzoic acid (m-CPBA); methyl (Me); methanol (MeOH); N-bromosuccinamide (NBS); N-methylmorpholine N-oxide (NMO); 1-hydroxybenzotriazole (HOBt); N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC); diisopropyl azodicarboxylate (DIAD); lithium diisopropylamide (LDA); triethyl amine (TEA); di-tert- butydicarboxylate (BOC20); glycine methyl ester (Gly-OMe); dimethyl sulfide (DMS);
dichloroethane (DCE); N-iodosuccinamide (NIS); trifiic acid or trifluoromethanesulfonic acid (TfOH); N-methyl morpholine (NMP); methanesulfonyl chloride (MsCl); 1,8- diazabicyclo[5.4.0]-undec-7-ene (DUB); p-toulenesulfonylmethyl isocyanide (TosMIC);
dimethoxyethane (DME); tetramethyl ethylene diamine (TMEDA); 4-dimethylamino pyridine (DMAP); Pd2dba3 (Tris(dibenzylideneacetone)dipalladium(O)); Xantphos (4,5-
Bis(diphenylphosphino)-9,9-dimethylxanthene; gram(s) (g); milligram(s) (mg); microliter(s) (μ ); milliliter(s) (mL); millimole (mmol); minute(s) (min, mins); hour(s) (h, hr or hrs); retention time (Rt); room temperature (rt, r.t. or RT); round bottom (RB); thin layer chromatography (TLC); flash chromatography (FC); liquid chromatography-mass spectrometry (LCMS or LC- MS); supercritical fluid chromatography (SFC); Medium Pressure Liquid Chromatography (MPLC); High Pressure Liquid Chromatography (HPLC); mass spectrum (ms or MS). Celite is the tradename for diatomaceous earth filter aid.
The following are representative procedures for the preparation of the compounds used in the following Examples, or which can be substituted for the compounds used in the following Examples which may not be commercially available.
INTERMEDIATE 1
Figure imgf000019_0001
(1 -Oxo-1 ,3-dihvdro-2-benzofuran-5-yl)acetaldehvde
Step A: 5 -( 1 ,3 -Dioxolan-2-ylmethyl -2-benzofuran- 1 (3H)-one : A three-neck 5L round bottomed flask equipped with a stir bar, firestone valve, thermocouple, condenser and heating mantle was charged with tri-t-butyl phosphonium tetrafluoroborate (500 mg, 1.72 mmol), palladium (II) acetate (250 mg, 1.1 mmol) and 5-bromo-2-benzofuran-l(3H)-one (100 g, 470 mmol). DMF (1.88 L) was added to the flask, and the mixture was degassed three times by alternating vacuum and nitrogen purge. Commercially available bromo(l ,3-dioxolan-2-ylmethyl)zinc solution (1.03 L, 516 mmol) was added via canula and the mixture was again degassed three times. The mixture was then heated at 85 °C for 5 hours, checked by HPLC-MS, then stirred at 85 °C for 5 more hours. The mixture was then allowed to return to RT for overnight. 2-MethylTHF (2L) and brine were added, and the mixture was stirred for 5 min. The layers were separated and the aqueous layer was extracted again with 2-methylTHF. The organic layers were combined, washed three times with brine (4L each), dried over MgS04, filtered and concentrated. The crude product was purified by flash chromatography (1.5 kg silica cartridge), eluting with 0-20% ethyl acetate in DCM to afford the title compound. LC-MS (IE, m/z): 221 [M+l ]+.
Step B: (1-Oxo-l J-dmvdro-2-benzofuran-5-vnacetaldehyde: 5-(L3-Dioxolan-2-ylmethyl -2- benzofuran-l(3H)-one (61 g, 280 mmol) was combined with water (2.2 L) in a 5 L round bottomed flask equipped with a Claisen adapter, thermocouple, stir bar and nitrogen bubbler. Aqueous HCl solution (2M, 1.14 L, 2.29 mol) was added and the resulting mixture was heated at 40 °C for 8 h. Then the mixture was stirred overnight at room temperature. The mixture was extracted three times with 2 L of ethyl acetate. The combined organic layers were concentrated to give (l-oxo-l,3-dihydro-2-benzofuran-5-yl)acetaldehyde. LC-MS (IE, m/z): 177 (M+l)+.
Figure imgf000020_0001
2-(2-Oxopiperazin- 1 -yl)quinoline-6-carbonitrile
Step A: 2-chloroquinoline-6-carbonitrile: To a flask containing a stir bar was added 6-bromo-2- chloroquinoline (0.90 g, 3.7 mmol), CuCN (0.50 g, 5.6 mmol) followed by addition of DMF (10 mL). The resulting mixture was then refluxed at 150 °C overnight. When the reaction appeared complete by LC analysis, the reaction flask was taken out of the oil bath and cooled to room temperature. To the reaction mixture was then poured DCM (20 mL) and a precipitate formed immediately, which was filtered, redissolved in DCM, absorbed onto silica gel, and purified by flash chromatography to provide the title product.
Step B: tert-Butyl 4-(6-cvanoquinolin-2-yl')-3-oxopiperazine-l-carboxylate:
To a 25 mL flask was added a stir bar, 2-chloroquinoline-6-carbonitrile (0.015 g, 0.79 mmol), Pd2dba3 (0.072 g, 0.08 mmol), Xantphos (0.09 g, 0.16 mmol), cesium carbonate (0.36 g, 1.13 mmol) and tert-Butyl 3-oxopiperazine-l-carboxylate (0.31 g, 1.6 mmol). The resulting mixture was degassed and purged with N2 (3X). To the flask was added 1,4-dioxane (10 mL). The reaction mixture was then refluxed at 100 °C overnight. Analysis of the reaction mixture by LC indicated that reaction had gone to completion. The solution was concentrated in vacuo and the resulting crude was dissolved in EtOAc and washed with brine, dried over Na2S04, filtered and concentrated in vacuo. The crude residue was purified by silica gel column chromatography (10% MeOH in DCM) to provide the title product.
Step C: 2-(2-Oxopiperazin-l-yl)quinoline-6-carbonitrile: tert-Butyl 4-(6-cyanoquinolin-2-yl)-3- oxopiperazine-l-carboxylate (0.15 g, 0.79 mmol) was treated with TFA (2 mL) and stirred for 20 minutes. Analysis of the reaction mixture by LC indicated the reaction had gone to completion. The solution was concentrated in vacuo and crude was azetroped (3X) with dichloroethane to furnish the title product. LC MS (IE, m/z): [M+l]+ = 253.
INTERMEDIATE 3
Figure imgf000021_0001
7-Methyl-2-(2-oxopiperazin- 1 -yl quinoline-6-carbonitrile
Step A: 7-Methyl-2-oxo-l, 2-dihydroquinoline-6-carbonitrile
A mixture of 6-bromo-7-methylquinolin (10 g, 42.0 mmol, prepared following J Med. Chem. 1988, 31, 2048.), N-methyl-2-pyrrolidinone (32 mL), and CuCN (1 1 g, 0.13 mol) was stirred at 20 °C overnight and then at reflux temperature (202 °C) for 7 h. The mixture was poured into water (250 mL) and filtered, and the solid was washed twice with water to furnish 7-Methyl-2- oxo-1 , 2-dihydroquinoline-6-carbonitrile.
Step B: 2-Chloro-7-methylquinoline-6-carbonitrile: A mixture of 7-Methyl-2-oxo-l, 2- dihydroquinoline-6-carbonitrile (10 g, 54 mmol) and POCl3 (30 mL) was stirred at 110 °C for 2 h and then at 20 °C overnight. The mixture was carefully poured into a mixture of water and ice (400 mL) while being stirred vigorously. The product was collected by filtration and dried by co- evaporation with MeCN/Toluene to furnish the title product. LC MS (IE, m/z): [M+l]+ = 203. Step C: tert-Butyl 4-(6-cvano-7-methylquinolin-2-yl)-3-oxopiperazine-l-carboxylate: To a 25 mL flask was added a stir bar, 2-Chloro-7-methylquinoline-6-carbonitrile (0.2 g, 0.98 mmol), Pd2dba3 (0.11 g, 0.19 mmol), Xantphos (0.090 g, 0.090 mmol), Cesium Carbonate (0.26 g, 0.63 mmol) and tert-Butyl 3-oxopiperazine-l-carboxylate (0.29 g, 1.4 mmol). The resulting mixture was degassed and purged with N2 three times. To the flask was added 1 ,4-dioxane (4 mL). The reaction mixture was then refluxed at 100 °C overnight. The solution was concentrated in vacuo and the resulting crude was dissolved in EtOAc and washed with brine, dried over Na2S04, filtered and concentrated in vacuo. The crude residue was then purified by silica gel column chromatography (10% MeOH in DCM) to provide the title compound.
Step D: 7-Methyl-2-(2-oxopiperazin-l-yl) quinoline-6-carbonitrile: tert-Butyl 4-(6-cyano-7- methylquinolin-2-yl)-3-oxopiperazine-l-carboxylate was treated with TFA (2 mL) and stirred for 20 minutes. Analysis of the reaction mixture by LC indicated that reaction had gone to completion. The solution was concentrated in vacuo and crude was azetroped (3X) with dichloroethane to furnish the title compound.LC/MS (IE, m/z)' [M+l]+ = 267.
INTERMEDIATE 4
Figure imgf000022_0001
3 -(2-Oxopiperazin- 1 -yl quinoline-7-carbonitrile
Step A: 3-Chloroquinoline-7-carbonitrile: To a flask containing a stir bar was added 7-Bromo-3- chloroquinoline (1.0 g, 4.1 mmol), CuCN (0.55 g, 6.2 mmol), and DMF (25 mL). The mixture was then refluxed at 150 °C overnight. When the reaction was complete, as evidenced by LC analysis, the reaction flask was taken out of the oil bath and cooled to room temperature. To the reaction mixture was then poured DCM (20 mL) and a precipitate formed immediately, which was filtered off. The crude product was adsorbed onto silica gel and purified by silica gel column chromatography (HexanesiDCM→ 1 :1) to provide the title product.
Step B: tert-Butyl 4-(7-cyanoquinolin-3-yl)-3-oxopiperazine-l-carboxylate
To a 25 mL flask was added a stir bar, 7-Chloroquinoline-3-carbonitrile (0.36 g, 1.9 mmol), Pd2dba3 (0.17 g, 0.19 mmol), Xantphos (0.22 g, 0.38 mmol), cesium carbonate (0.70 g, 2.3 mmol) and tert-butyl 3-oxopiperazine-l-carboxylate (0.45 g, 2.3 mmol). The resulting mixture was degassed and purged with N2 (3X). To the flask was added 1,4-dioxane (0.75 mL). The reaction mixture was then heated to 100 °C overnight. The solution was concentrated in vacuo and the resulting crude was dissolved in EtOAc and washed with brine, dried over Na2S04, filtered and concentrated in vacuo. The crude residue was then purified by silica gel column chromatography (5% MeOH in DCM) to provide the title product.
Step C: 3-(2-Oxopiperazin-l-yl) quinoline-7-carbonitrile: tert-Butyl 4-(7-cyanoquinolin-3-yl)-3- oxopiperazine-l-carboxylate (0.030 g, 0.099 mmol) was treated with TFA (2 mL) and stirred for 20 minutes. Analysis of the reaction mixture by LC indicated that reaction had gone to completion. The solution was concentrated in vacuo and crude was azetroped with
dichloroethane (3 X 8 mL) to furnish the title product. LC/MS (IE, m/z): [M+l]+ = 253.
Figure imgf000022_0002
6-(2-Oxopiperazin-l -yl)naphthalene-2-carbonitrile hydrochloride
Step A: tert-Butyl 4-(6-bromo-2-naphthyl -3 -oxopiperazine-l-carboxylate: To a microwave tube charged with a stir bar was added 2,6-dibromonaphthalene (710 mg, 2.5 mmol), tert-butyl 3- oxopiperazine-l-carboxylate (250 mg, 1.2 mmol), copper(I) iodide (48 mg, 0.25 mmol), Ν,Ν'- dimethylcyclohexane-l,2-diamine (71 mg, 0.50 mmol), K3P04 (530 mg, 2.5 mmol), and dioxane (10 mL). The mixture was heated to 150 °C in a microwave reactor for 1 hour. The reaction was diluted with EtOAc, adsorbed onto silica gel, and purified by MPLC (silica gel, EtOAc-Hexanes) to afford the title compound. LC-MS (IE, m/z): 407 [M + 1]+.
Step B: 6-(2-Oxopiperazin-l-yl)naphthalene-2-carbonitrile hydrochloride: To a flask charged with tert-butyl 4-(6-bromo-2-naphthyl)-3 -oxopiperazine-l-carboxylate (500mg, 1.2 mmol) and a stir bar was added copper(I) cyanide (220mg, 2.5 mmol) and DMF (20 mL). The mixture was heated to 135 °C for 12 hours under an atmosphere of N2. The reaction was diluted with EtOAc, washed with sat. N¾OAc and brine, dried over sodium sulfate, concentrated and purified by silica gel chromatography. The resulting product was treated with 4N HC1 in dioxane, and removal of solvent furnished the title compound. LC-MS (IE, /z): 252 [M + 1]+. The salt was further washed with aqueous sodium carbonate, and back extracted with CHCI3-IPA (3:1).
INTERMEDIATE 6
Figure imgf000023_0001
3-(2-oxopiperazin- 1 -vnisoquinoline-7-carbonitrile
Step A: 3-Chloroisoquinoline-7-carbonitrile: To a flask charged with 7-bromo-3- chloroisoquinoline (l.Og, 4.1 mmol) and a stir bar was added copper(I) cyanide (550mg, 6.2 mmol) and DMF (50 mL). The mixture was heated to 150 °C for 24 hours under an atmosphere of nitrogen. The reaction was diluted with EtOAc, washed with sat. NH4OAc and brine, dried over sodium sulfate, concentrated and purified by silica gel chromatography (Hexanes:DCM) and the solvent removed to afford the title compound. LC-MS (IE, m/z): 189 [M + 1]+.
Step B: 3-(2-oxopiperazin-l-vnisoquinoline-7-carbonitrile hydrochloride
To a flask charged with 3-chloroisoquinoline-7-carbonitrile (250mg, 1.3 mmol) and a stir bar was added tert-butyl 3 -oxopiperazine-l-carboxylate (400 mg, 2.0 mmol), Pd2(dba)3 (120 mg,
0.13 mmol), Xantphos (150 mg, 0.26 mmol), cesium carbonate (650 mg, 2.0 mmol), and dioxane (20 mL). The mixture was purged three times with nitrogen, and heated to 100 °C for 16 hours. The mixture was diluted with EtOAc, washed with brine, dried over sodium sulfate, adsorbed onto silica gel, and purified by silica gel chromatography (Hexanes:EtOAc) to provide tert-butyl 4-(7-cyanoisoquinolin-3-yl)-3-oxopiperazine-l-carboxylate. LC-MS (IE, m/z): 353 [M + 1]+. StepC: 3-(2-oxopiperazin- 1 -yl)isoquinoline-7-carbonitrile: The product of Step B was further treated with 4N HC1 in dioxane, and removal of solvent furnished 3-(2-oxopiperazin-l- yl)isoquinoline-7-carbonitrile hydrochloride. The HC1 salt was washed with aqueous sodium carbonate, and back extracted with CHC13-IPA (3:1) to furnish the title compound.
Figure imgf000024_0001
7-(2-oxopiperazin- 1 -yl naphthalene-2-carboriitrile
Step A: tert-Butyl 4-(7-bromo-2-naphthvn-3-oxopiperazine-l-carboxylate: To a 20 mL microwave vial charged with 2,7-dibromonaphthalene (1.1 g, 3.8 mmol), tert-butyl 3- oxopiperazine-l-carboxylate (0.50 g, 2.5 mmol), and a stir bar was added copper (I) iodide (95 mg, 0.50 mmol), N,N'-dimethylcyclohexane-l,2-diamine (142 mg, 1.0 mmol), K3P04 (1 100 mg, 5.0 mmol), and dioxane (15 mL). The mixture was purged three times with nitrogen, and heated to 150 °C in a microwave reactor. The crude solution was diluted with EtOAc, adsorbed onto silica gel, and purified by silica gel chromatography (HexrEtOAc). The solvent was removed to provide the title compound. LC-MS (IE, m/z): 405 [M + 1]+.
Step B: 7-(2-oxopiperazin-l-yl')naphthalene-2-carbonitrile
To a flask charged with tert-Butyl 4-(7-bromo-2-naphthyl)-3-oxopiperazine-l-carboxylate (450 mg, 1.1 mmol) and a stir bar was added copper (I) cyanide (300 mg, 3.3 mmol) and DMF (10 mL). The mixture was heated to 140 °C for 16 hours. The reaction was diluted with DCM. The solids were filtered off. The filtrate was concentrated, adsorbed onto silica gel, and purified by silica gel chromatography (Hex:EtOAc 1 :1) to afford tert-butyl 4-(7-bromo-2-naphthyl)-3- oxopiperazine-l -carboxylate. LC-MS (IE, m/z) 352 [M + 1]+. The product was further treated with TFA to remove the Boc group. The resulting TFA salt was washed with aqueous sodium carbonate, and back extracted with CHCI3-IPA (3:1) to furnish the title compound.
Figure imgf000024_0002
2-(2-oxopiperazin-l -yl)-l ,3-benzothiazole-5-carbonitrile
To a flask charged with 2-chloro-l,3-benzothiazole-5-carbonitrile [prepared from 3-amino-4- chlorobenzonitrile following the procedure described in US Publication 2009/0197862, Example 267] (146 mg, 0.75 mmol) and a stir bar was added tert-butyl 3 -oxopiperazine-1 -carboxylate (225 mg, 1.1 mmol), Pd2(dba)3 (69 mg, 0.075 mmol), Xantphos (87 mg, 0.15 mmol), cesium carbonate (370 mg, 1.1 mmol), and dioxane (10 mL). The mixture was purged three times with nitrogen, and heated to 100 °C under N2 for 16 hours. The reaction was diluted with EtOAc, adsorbed onto silica gel, and purified by silica gel chromatography (Hexanes:EtOAc 1 : 1) to provide tert-butyl 4-(5-cyano-l, 3 -benzothiazol-2-yl)-3 -oxopiperazine-1 -carboxylate (95 mg, 35% yield). The carboxylate was further treated with TFA to remove the Boc group. After removal of TFA, the residue was dissolved in aqueous sodium carbonate, extracted with DCM, dried over sodium sulfate, and concentrated to provide the free base of 2-(2-oxopiperazin-l-yl)-l,3- benzothiazole-5-carbonitrile. LC-MS (IE, m/z): 259 [M + 1 ]+.
Figure imgf000025_0001
7-(2-oxopiperazin- 1 -yl)quinoline-3 -carbonitrile
Step A: 7-chloroquinoline-3-carbonitrile: To a flask containing a stir bar was added 3-bromo-7- chloroquinoline (1.0 g, 4.1 mmol), CuCN (0.55 g, 6.2 mmol) followed by addition of DMF (25 mL). The resulting mixture was then heated to 150 °C overnight. When the reaction was complete as evidenced by LC analyisis, the reaction flask was taken out of the oil bath and cooled to RT. To the reaction mixture was then poured DCM (20 mL) and a precipitate formed immediately, which was filtered. The filtrated was washed with aq. NH4CI, dried over sodium sulfate, and purified by flash chromatography. LC-MS (IE, m/z) 189 [M + 1]+.
Step B: tert-butyl 4-(3-cvanoquinolin-7-yl)-3-oxopiperazine-l-carboxylate
To a 25 mL flask was added a stir bar, 7-cUoroquinoline-3 -carbonitrile (0.15 g, 0.79 mmol), Pd2dba3 (0.072 g, 0.08 mmol), Xantphos (0.090 g, 0.16 mmol), cesium carbonate (0.36 g, 1.13 mmol) and tert-butyl 3 -oxopiperazine-1 -carboxylate (0.31 g, 1.6 mmol). The resulting mixture was degassed and purged with N2 (3X). To the flask was added 1, 4 dioxane (10 mL). The resulting reaction mixture was then refluxed at 100 °C for overnight. Analysis of the reaction mixture by LC indicated that reaction had gone to completion. The solution was concentrated in vacuo and the resulting crude was dissolved in EtOAc and washed with brine, then dried over Na2S04, filtered and concentrated in vacuo. The crude residue was then subjected for purification by silica gel column chromatography (10% MeOH in DCM) to provide the title product. Step C: 7-(2-oxopiperazin-l-yl)quinoline-3-carbonitrile: tert-Butyl 4-(3-cyanoquinolin-7-yl)-3- oxopiperazine-l-carboxylate was treated with TFA (2 mL) for 20 minutes at RT. The TFA was removed under vacuum, and the residue was dissolved in aqueous sodium carbonate, extracted with DCM, dried over sodium sulfate, and concentrated to deliver the free base of 7-(2- oxopiperazin-l-yl)quinoline-3-carbonitrile. LC-MS (IE, m/z): 253 [M + 1]+.
INTERMEDIATE 10
Figure imgf000026_0001
4-r(2i?>2-Hydroxy-2-(4-methyl-l-oxo-l -dihydro-2-berizofuran-5-yDethyl1piperazin-2-one A mixture of 4-Methyl-5-[(2i?)-oxiran-2-yl]-2-benzofuran-l(3H)-one (0.46 g, 2.4 mmol) and piperazin-2-one (0.20 g, 2.0 mmol) was heated to 145 °C in a microwave tube for 1 hour. The crude reaction was diluted with DCM, adsorbed onto silica gel, and purified by silica gel chromatography to provide the the title product. LC-MS (IE, m/z): 291 (M+l)+.
I 1
Figure imgf000026_0002
6-Bromo- 1 -benzothiophene-2-carbonitrile
A mixture of 6-bromo-l-benzothiophene-2-carboxylic acid (prepared in a similar manner to J. Med. Chem.(2007), 50(20), 4793-4807) (240mg, 0.95mmol) and thionyl chloride was heated to 75 °C in 10 mL of DCE until the mixture becomes homogeneous, about 1 hour. The reaction was then concentrated to dryness and redissolved in lOmL of DCE and then excess 2N ammonia in MeOH was added (about lOmL) and the reaction stirred at room temp for 30 minutes before excess solvent was removed. The crude amide was then redissolved in DCM, the salts were filtered off and the filtrate concentrated. The crude amide was then taken up in phosphorus oxychloride (lOmL) and heated at 80 °C for 2 hours and then cooled to RT. The reaction was partitioned between DCM and water, the organic layer was then washed with brine, dried over magnesium sulfate, filtered and purified via MPLC (0- 10% EtO Ac/Hex) to give 6-Bromo- 1 - benzothiophene-2-carbonitrile. 1H NMR (500 MHz; CDC13): 8.06 (d, J= 1.4 Hz, 1H), 7.88(s, 1H), 7.78 (d, J = 8.6 Hz, 1H), 7.62 (dd, J= 1.4, 8.6 Hz, 1H). LC-MS (IE, /z): 238 [M+l]+.
INTERMEDIATE 12
Figure imgf000027_0001
3-Chloro-8-methylisoquinoline-7-carbonitrile
Step A: 7-bromo-3-chloro-8-iodoisoquinoline
To a flask containing 7-Bromo-3-chloroisoquinoline (1.5 g, 6.19 mmol) was added NIS (1.4 g, 6.9 mmol). The flask was placed in an ice bath and treated with TfOH (40 mL) and stirred at RT overnight. The reaction mixture was carefully poured into ice, and the aqueous layer was extracted with DCM. The combined extracts were washed with brine, dried over Na2S04, and concentrated under reduced pressure. The crude residue was purified by silica gel column chromatography (Hexanes:DCM→ 1 : 1) to provide the title product.
Step B: 7-Bromo-3-chloro-8-methylisoquinoline
To a 20 mL microwave tube was added a stir bar, 7-bromo-3-chloro-8-methylisoquinoline (0.81 g, 2.2 mmol), Pd2dba3 (0.20 g, 0.22 mmol) and triphenylphosphine (0.12 g, 0.44 mmol). The tube was capped, degassed and then purged with N2 and heated in a microwave reactor at 50 °C for 10 minutes. The cap was removed, and the tube treated with copper (I) iodide (0.04 g, 0.22 mmol), capped, degassed and purged with N2 and heated again 50 °C for 10 minutes. The cap was once again opened and to the tube was added tetramethyltin (0.30 mL, 2.4 mmol), which was followed by capping, degassing and purging of the reaction mixture with N2, and heating it to 120 °C for 30 minutes. To the mixture was added water, and the aqueous layer was extracted with DCM. The combined extracts were washed with brine, dried over Na2S04, and concentrated under reduced pressure. The crude residue was purified by silica gel column chromatography (Hexanes:DCM→ 1 :1) to provide the title product. LC/MS (IE, m/z): [M+l]+ = 257.
Step C: 3-Chloro-8-methylisoquinoline-7-carbonitrile
To a flask containing a stir bar was added 7-Bromo-3-chloro-8-methylisoquinoline (0.63 g, 2.4 mmol), CuCN (0.33 g, 3.7 mmol), and DMF (10 mL). The mixture was then refluxed at 150 °C overnight. When the reaction was complete, as evidenced by LC analysis, the reaction flask was taken out of the oil bath and cooled to room temperature. To the mixture was then poured DCM (20 mL) and a precipitate formed immediately, which was filtered, re-dissolved in DCM, adsorbed into silica gel and purified by silica gel column chromatography (Hexanes:DCM→ 1 :1) to provide the title product. LC/MS (IE, m/z): [M+l]+ = 203.
INTERMEDIATE 13
Figure imgf000028_0001
6-Chlorothieno[2.3-blpyridine-2-carbonitrile
Step A: 6-Chloro-2-iodothieno[Z3-b1pyridine: To a flask charged with 6-Chlorothieno[2,3- b]pyridine (500 mg, 3.0 mmol) and a stir bar was added NIS (800 mg, 3.5 mmol) and Triflic acid (5 mL). The mixture was monitored by TLC and allowed to stir at RT for 4 hours. The reaction was poured into ice, neutralized with aqueous sodium carbonate, extracted with DCM, dried over sodium sulfate, concentrated and purified by flash chromatography (Hex: EtOAc gradient) to obtain the title product. LC-MS (IE, tn/z): 296 [M + 1]+.
Step B: 6-Chlorothieno[2,3-b1pyridine-2-carbonitrile
A mixture of 6-Chloro-2-iodothieno[2,3-b]pyridine (80 mg, 0.27 mmol) and Copper (I) Cyanide (36 mg, 0.41 mmol) in DMF (3 mL) was heated to 130 °C for 16 hrs. LC showed formation of the title product. The crude reaction was adsorbed onto silica gel, and purified by flash chromatography to obtain the title product. LC-MS (IE, m/z): 195 [M + 1]+.
INTERMEDIATE 14
Figure imgf000028_0002
6-Bromo-2-(methylsulfonyl quinoline
Step A: 6-Bromo-2-(methylsulfanyl quinoline: A solution of 6-Bromo-2-chloroquinoline (0.68 g, 2.8 mmol) in DMF was treated with sodium thiomethoxide (0.22 g, 3.1 mmol). After 1 h at RT, the LC-MS indicated conversion. The solution was diluted with brine and extracted with EtOAc. The organic layer was removed, dried, filtered and concentrated. The crude product was used in the next step. LC-MS (IE, m/z): 256 [M + 1]+.
Step B: 6-Bromo-2-(methylsulfonyl)quinoline:
A stirred solution of 6-Bromo-2-(methylsulfanyl)quinoline in DCM was treated with m-CPBA (1.07 g, 6.2 mmol) at RT. After 1 hour, TLC and LC-MS indicated conversion. The solvent was removed and the material was dissolved in minimal DCM and purifed via MPLC. The title compound eluted with 0-60% EtOAc, and the solvent was removed to obtain the title product. LC-MS (IE, m/z): 288 [M + 1]+.
INTERMEDIATE 15A and 15B
Figure imgf000029_0001
I- 15 A: 4-Methyl-5-[(2^)-oxiran-2-yl]-2-benzofuran-l(3H)-one and
I- 15B: 4-Methyl-5-rr25 -oxiran-2-yll-2-benzofuran- 1 f 3- fl-one
Step A: (3-Bromo-2-methylphenyl methanol: To a solution of 3-bromo-2-methyl benzoic acid (35 g, 163 mmol) in THF (200 mL) was added Borane THF Complex (1.0 M, 212 mL, 212 mmol). The mixture was allowed to stir for 24 h. The reaction was then quenched with water. The THF was removed under reduced pressure. The resulting solid was dissolved in ethyl acetate (500 mL), washed with IN HC1, sodium carbonate, and brine. The organic layer was dried over sodium sulfate and concentrated to afford (3-bromo-2-methylphenyl)methanol.
Step B: 5-Bromo-4-methyl-2-benzofuran-l(3H)-one: To a flask charged with (3-bromo-2- methylphenyl)methanol (6.0 g, 30 mmol) was added a 1M TFA solution of Thallium
Trifluoroacetate (16.2 g, 29.8 mmol). The mixture was stirred at RT overnight. The solvent was removed under vacuum, and the residue was pumped under high vacuum for 30 min to ensure complete removal of TFA. To the residue was then added palladium(II) chloride (529 mg, 2.98 mmol), lithium chloride (2.53 g, 59.7 mmol), magnesium oxide (2.41 g, 59.7 mmol), and MeOH (150 mL). The reaction was flushed with CO twice, and kept under CO at RT. Analysis by LC showed a formation of product within 2 hours. To this solution was added ethyl acetate to precipitate the salts. The solution was filtered through a celite pad, washed with EtOAc, adsorbed onto silica and purified by silica gel chromatography to provide the title compound Step C: 5-ethenyl-4-methyl-2-benzofuran-U3H -one: 5-Bromo-4-methyl-2-benzofuran-l(3H)- one (598 mg, 4.47 mmol), potassium vinyl trifluoroborate (507 mg, 2.23 mmmol), PdCl2(dppf)- CH2C12 Adduct (182 mg, 0.223 mmmol) , and TEA (0.622 mL, 4.47 mmol) were added to 10 mL ethanol in a 20 mL microwave tube. The tube was sealed and degassed, then heated to 140 °C for 20 min. Analysis by LC-MS showed product peak. The reaction mixture was diluted with ethyl acetate, washed with brine twice, dried and evaporated to dryness. The crude product was purified by MPLC chromatography using a 120g Redi-sep column and 0-80% EtOAC/Hexane solvent system to yield the title product. LC-MS: M+l= 175 at 2.42 retention time.
Step D: 4-methyl-5-oxiran-2-yl-2-benzofuran-l(3H)-one
5-ethenyl-4-methyl-2-benzofuran-l(3H)-one (1.46 g, 8.38 mmol) was added to DCM (25 mL) at 0 °C then m-CPBA (2.89 g, 16.8 mmol) was added and the mixture was stirred at RT overnight. The reaction mixture was washed once each with saturated aqueous Na2S203, NaHC03, and brine. The organic layer was dried over Na2S04, filtered, and evaporated to dryness. The crude material was purified by MPLC chromatography through 120g Redi-sep column eluting with 0- 80% EtOAc/hexane solvent system to yield 4-methyl-5-oxiran-2-yl-2-benzofuran-l(3H)-one. 1H- NMR (500 MHz, CDC13): δ ppm 7.77 ( d, J= 8 Hz, 1H), 7.43 (d, J= 8 Hz, 1H), 5.30 (s, 2 H), 4.12 ( s, 1 H), 3.27 (t, J= 4Hz, 1 H), 2.735 ( dd, J = 2.2, 5.5 Hz, 1H) , 2.43 (s, 3H).
Step E: 1-15 A (slower eluting isomer and I-15B (faster eluting isomer):
Racemic 4-methyl-5-oxiran-2-yl-2-ben^ofuran-l(3H)-one was resolved on a ChiralPak® AD-H column (5x25cm) under SFC conditions on a Berger MGIII preparative SFC instrument. The racemate was diluted to 50 mg/ml in 1 :1 DCM:MeOH. The separation was accomplished using 10% EtOH/C02, flow rate 200 ml/min, 100 bar, 25 °C. 500 ul Injections were spaced every 2.12 mins. The faster eluting (2i?)-epoxide (I-15B) eluted at 5.2 min, and the slower eluting (25)- epoxide (I- 15 A) eluted at 5.6 min.
IN 16
Figure imgf000030_0001
6-Methyl-5-(oxiran-2-yl)-2-benzofuran- 1 (3H)-one
Step A: 5-Prop-2-en-l-yl-2-benzofuran-l(3H)-one: A mixture of 5-bromo-2-benzofuran-l(3H)- one (15.0 g, 70.4 mmol), allyl-tributyl-stannane (25.6 g, 77.5 mmol), LiCl (11.8 g, 282 mmol) and Pd(PPh3)4 (1.2 g, 1.0 mmol) in 100 mL toluene was heated under N2 at 90-100 °C overnight. After cooling to RT, the mixture was diluted with 250 mL EtOAc and filtered. The filtrate was washed with water and brine, dried over anhydrous Na2S04 and concentrated to dryness. The residue was purified via column (DCM/Pet Ether =1 :5) to give the title compound.
Step B: 5-(2-hvdroxyethyl)-2-benzofuran-l(3H)-one
To a solution of 5-prop-2-en-l-yl-2-benzofuran-l(3H)-one (13.5 g, 45.2 mmol) in 200 mL DCM/MeOH (V/V=l : 1 ) was bubbled 03 at -78 °C for 30 min, and N2 was bubbled for another 15 min at -78 °C. Then 20 mL of Me2S were added, and the mixture was stirred at RT overnight before concentrating to dryness. The residue was dissolved in MeOH (100 mL) and then cooled to 0 °C. NaBHt (5.90 g, 155 mmol) was added in portions. The resulting mixture was stirred at 0 °C for 1 h, then quenched with citric acid (aq.) and extracted three times with EtOAc. The combined organic layers were washed with NaHC03 (aq.) and brine, dried over anhydrous Na2S04 and concentrated to dryness. The residue was purified via column chromatography (EtOAc/ Pet Ether =1 :5) to give 5-(2-hydroxyethyl)-2-benzofuran-l(3H)-one.
Step C: 5-(2-hydroxyethyl -6-iodo-2-benzofuran-U3 /)-one
To a cooled (0 °C) solution of 5-(2-hydroxyethyl)-2-benzofuran-l(3H)-one (9.00 g, 50.6 mmol) in 100 mL of TfOH was added NIS (12.5 g, 55.6 mmol), then the mixture was stirred at 0 °C for 2 hrs and then poured into ice- water (500 mL). The solution was extracted three times with 500 mL of EtOAc and the combined organic layers were washed with saturated NaHC03 and brine, dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by column chromatography (EtOAc/ Pet Ether =1 :5) to give the title compound.
Step D: 5-(2-hydroxyethyl -6-methyl-2-benzofuran-l(3H)-one: To a flask charged with 5-(2- hydroxyethyl)-6-iodo-2-benzofuran-l(3H)-one (6.00 g, 19.7 mmol) and a stir bar was added Pd2(dba)3 (452 mg,0.493 mmol), PPh3 (1 g, 4 mmol) and NMP (50 mL). The mixture was purged with N2 and heated to 50 °C for 10 min, followed by addition of Cul (375 mg, 1.97 mmol). After the mixture was heated for another 10 min, Sn(CH3)4 (5.30 g, 29.6 mmol) was added into the reaction, and it was heated to 120 °C for 2 h. After cooling to RT, the mixture was diluted with saturated N¾C1 (200 mL) and extracted with EtOAc (3x200 mL). The combined organic layers were washed with water and brine, dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by prep-HPLC to give the title compound. Step E: 2-(6-methyl-l-oxo-L3-dihydro-2-benzofuran-5-yl ethyl methanesulfonate
To a solution of 5-(2-hydroxyemyl)-6-memyl-2-berizofuran-l (3H)-one (1.20 g, 6.25 mmol) and TEA (2.5 g, 25 mmol) in DCM (100 mL) was added MsCl (1.40 g, 12.5 mmol) at 0 °C. The mixture was stirred at ambient temperature overnight, then was washed with water and brine. The organic layer was dried and concentrated to dryness to obtain the title compound.
Step F: 5-e1henyl-6-methyl-2-benzofuran-l(3H)-one
To a mixture of 2-(6-methyl-l-oxo-l,3-dihydro-2-benzofuran-5-yl)ethyl methanesulfonate (2.00 g, 7.41 mmol) and TEA (5 mL) in DCM (50 mL) was added DBU (5 mL) slowly at 0 °C. The mixture was stirred at r.t. overnight, and then was diluted with 50 mL of DCM, washed with 2 N HCl three times and brine. The organic layer was dried and concentrated to dryness. The residue was purified by prep-TLC to give 5-ethenyl-6-methyl-2-benzofuran-l(3H)-one.
Step G: 6-methyl-5-oxiran-2-yl-2-benzofuran-l('3H)-one
To a solution of 5-ethenyl-6-methyl-2-benzofuran-l(3H)-one (1.00 g, 5.75 mmol) in 50 mL of DCM was slowly added m-CPBA (3.50 g, 17.4 mmol) in 50 mL of DCM at 0 °C. The mixture was warmed to room temperature, and stirred for 2 days. The mixture was washed with aqueous Na2S03 until KI indicator paper didn't change color. The organic layer was washed with brine and then concentrated. The residue was purified via silica column to give product 6-methyl-5- oxiran-2-yl-2-benzofuran-l(3H)-one. LC/MS (IE, m/z): [M+l]+ = 191.
I 7
Figure imgf000032_0001
6-Oxo-5,6J.8-tetrahvdronaphthalene-2-carbonitrile: To a 20 mL microwave tube was added 5- bromo-2-tetralone (2.0 g, 8.9 mmol) and Palladium(O) Tetrakis (0.616 g, 0.533 mmol), zinc cyanide (1.0 g, 8.9 mmol), and DMF (8 mL). The tube was sealed, degassed and microwaved at 80 °C for 1.0 hour. TLC showed no starting material left. The mixture was diluted with ethyl acetate, washed with ammonium hydroxide (2M, 2 x), dried with sodium sulfate, filtered and evaporated under reduced pressure. The product was purified by silica gel chromatography with isocrartic 30% ethyl acetate-hexane to furnish the title compound. 1H-NMR (500 MHz, CDC13) 6 ppm 7.57 (s, 1H), 7.55 (d, J= 7.8 Hz, 1H), 7.28 (d, J= 7.8 Hz, 1H), 3.67 (s, 2H), 3.14 (t, J= 7.0 Hz, 2H), 2.60 (t, J= 7.0 Hz, 2H).
I
Figure imgf000032_0002
Methyl 5-nitro-2-(2-oxoethyl benzoate
Step A: Methyl 5-nitro-2-(prop-2-en-l-vDbenzoate
A mixture of Methyl 2-bromo-5-nitrobenzoate (0.60 g, 2.3 mmol), allyl tri-n-butyltin (0.92 g, 2.8 mmol), lithium chloride (0.29 g, 6.9 mmol), and palladium tetrakis (0.13 g, 0.12 mmol) was heated to reflux for 16 hours in toluene. TLC showed formation of the desired product. The reaction was diluted with EtOAc, washed with brine, and separated. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by silica gel chromatography to obtain the the title compound.
Step B: Methyl 5-nitro-2-(2-oxoethyl)benzoate: To a solution of Methyl 5-nitro-2-(prop-2-en- 1 - yl)benzoate (0.20 g, 0.90 mmol) in water (2 mL) and THF (5 mL) was added osmium tetroxide (0.57 mL 2.5% solution, 0.045 mmol) and NMO (0.16 g, 1.4 mmol). The mixture was allowed to stir at RT for 16 hours and monitored by TLC. The reaction was diluted with EtOAc, washed with NH4C1 and brine, and concentrated. The residue was redissolved in MeOH and water. After cooling the solution to 0 °C, an aqueous solution of sodium periodate (0.39 g, 1.8 mmol) was dropped into the reaction and allowed to stir for 2 hours. The reaction was diluted with water, extracted with EtOAc, dried over sodium sulfate, and concentrated. The crude residue was purified by silica gel chromatography to provide Methyl 5-nitro-2-(2-oxoethyl)benzoate. 1H- NMR (400 MHz, CDC13) δ ppm 9.48 (S, 1H), 8.97 (s, 1H), 8.39 (d, J- 8.0 Hz, 1H), 7.46 (d, J= 8.0 Hz, 1H), 4.31 (s, 2H), 3.98 (s, 3H).
INTERMEDIATE 19
Figure imgf000033_0001
Methyl 2-nitro-5-(2-oxoethyl)benzoate
The title compound was prepared from Methyl 5-chloro-2-nitrobenzoate using essentially the same procedure as described for Intermediate 40. Ή-NMR (400 MHz, CDC13) δ ppm 9.84 (S, 1H), 7.98 (d, J = 8.0 Hz, 1H), 7.61 (s, 1H), 7.50 (d, J= 8.0 Hz, 1H), 3.98 (s, 3H), 3.94 (s, 2H).
I
Figure imgf000033_0002
(2-Methoxy-4-nitrophenyDacetaldehyde
The title compound was prepared from l-Bromo-2-methoxy-4-nitrobenzene using essentially the same procedure as described for Intermediate 40. Ή-NMR (500 MHz, CDC13) δ ppm 9.78 (S, 1H), 7.89 (d, J = 8.0 Hz, 1H), 7.78 (s, 1H), 7.35 (d, J= 8.0 Hz, 1H), 3.98 (s, 3H), 3.81 (s, 2H).
INTERMEDIATE 21
Figure imgf000033_0003
(2-Fluoro-4-nitrophenyl¼cetaldehyde
The title compound was prepared from l-Bromo-2-fluoro-4- using essentially the same procedure as described for Intermediate 40. ^-NMR (500 MHz, CDC13) δ ppm 9.86 (S, 1H), 8.10 (m, 1H), 8.01 (m, 1H), 7.43 (m, 1H), 3.97 (s, 2H).
INTERMEDIATE 22
Figure imgf000034_0001
3-Fluoro-4-nitrophenylacetaldehvde
The title compound was prepared from 4-Bromo-2-fiuoro-l -nitrobenzene using essentially the same procedure as described for Intermediate 40. ^- MR (500 MHz, CD3C1) δ 9.84 (s, 1H), 8.09-8.08 (m, 1H), 7.21-7.15 (m, 2H), 3.87 (s, 2H)
I
Figure imgf000034_0002
2-Methyl-4-nitrophenylacetaldehvde
The title compound was prepared from l-Bromo-2-methyl-4-nitrobenzene using essentially the same procedure as described for Intermediate 40. ^- MR (500 MHz, CD3C1) δ 9.76 (s, 1H), 8.074 (s, 1H), 7.32-7.30 (m, 2H), 3.86 (s, 2H), 2.61 (s, 3H)
I 4
Figure imgf000034_0003
1 -(2-Iodoethyl)-4-nitrobenzene: To a 0 °C solution of tiphenylphosphine (1.9 g, 7.2 mmol) and iidazole (489 mg, 7.18 mmol) in DCM (20 ml) was added iodine (1822 mg, 7.18 mmol) to form a solution. Small portions of 2-(4-ntrophenyl)ethanol (1.0 g, 6.0 mmol) was added to the suspension. TLC showed conversion to the desired product quickly. Hexane was added to the reaction to precipitate side products which were filtered off. The filtrate was concentrated in vacuo to obtain crude l-(2-iodoethyl)-4-nitrobenzene, which was purified by silica gel flash chromatography using Hexane and EtO Ac system. 'H-NMR (500 MHz, CD3C1) δ 8.22 (d, J = 8.5 Hz, 2H), 7.40 (d, J= 8.5 Hz, 2H), 3.41 (t, J= 7.0 Hz, 2H), 3.32 (t, J= 7.0 Hz, 2H).
I
Figure imgf000034_0004
5-(2-Bromoethyl)-2-benzofuran- 1 (3H)-one
Step A: 5-Allyl-2-benzofuran-l(3H -one A 4-neck, 22-L, round bottom flask equipped with a mechanical stirrer, thermocouple, nitrogen bubbler, and condenser was charged with 5-bromophthalide (650 g, 3.0 mol), allyltri-n-butyltin (1200 g, 3.6 mol), palladium tetrakis triphenylphosphine (100 g, 0.089 mol), lithium chloride (250 g, 5.9 mol) and toluene (8.8 L). The mixture was evacuated and flushed with nitrogen 3 times and then was stirred at 100 °C for 4 hours. After slowly cooling to ambient temperature, the mixture was filtered and concentrated. The resulting solid was purified by silica gel column chromatography (heptane: ethyl acetate, 0->40%) to provide 5-allyl-2-benzofuran-l(3H)-one. Step B: 5-(2-HydroxyethylV2-berizofuran-l(3H)-one: 5-allyl-2-benzofuran-l(3H)-one (1.53 g, 8.78 mmol) was dissolved in methanol (30 mL). THF was added to solubilize the starting material. The resulting mixture was cooled in a dry ice acetone bath (-78 °C) and ozone was bubbled into the reaction until the color of the mixture changed to orange. Nitrogen was bubbled into the reaction for one minute to remove the excess ozone. Sodium borohydride (0.65 g, 2.9 mmol) was added at -78 °C, and the reaction mixture was allowed to warm to ambient temperature. The reaction mixture was concentrated part way and then taken up in ethyl acetate and water. The layers were separated and the organic layer was washed with brine, dried over magnesium sulfate, filtered, and concentrated to provide the title compound.
Step C: 5-(2-Bromoetnyl -2-benzofuran-l('3H)-one
To a solution of 5-(2-hydroxyethyl)-2-benzofuran-l(3H)-one (1.2 g, 6.8 mmol) in DCM at 0 °C was added carbon tetrabromide (2.3 g, 6.8 mmol), triphenylphosphine (1.8 g, 6.8 mmol), and imidazole (0.46 g, 6.8 mmol). The mixture was allowed to stir at 0 °C for 5 minutes, and then allowed to warm to RT and stir for 1.5 hours. The crude was concentrated and purified by silica gel chromatography (43% EtOAc with Hexanes) to obtain 5-(2-Bromoethyl)-2-benzofuran- l(3H)-one. LC-MS (IE, m/z): 241/243 (M+l)+.
I 6
Figure imgf000035_0001
(4-chloro-l -oxo-1 -dihydro-2-benzofuran-5-yl)acetaldehvde
Step A: methyl 3-amino-2-chlorobenzoate: To a solution of methyl 2-chloro-3-nitrobenzoate (2.1 g, 9.7 mmol) in methanol (100 mL) and THF (20 mL) was added zinc powder (1.9 g, 29 mmol), ammonium formate (3.1 g, 49 mmol), and a few drops of acetic acid. The mixture was allowed to stir at RT for 18 hours. Most of the volatiles were removed under reduced pressure. The residue was redissolved in EtOAc (200 mL), washed with brine, concentrated and purified by MPLC to provide methyl 3-amino-2-chlorobenzoate.
Step B: methyl 3-bromo-2-chlorobenzoate: To a solution of methyl 3-amino-2-chlorobenzoate (2.0 g, 11 mmol) in 48% HBr (10 mL) and water (20 mL) was added an aqueous solution of sodium nitrite (0.89 g, 13 mmol) at 0 °C. The mixture was allowed to stir at 0 °C for 30 minutes before it was added into a suspension of copper(I) bromide (2.3 g, 16 mmol) in water (10 mL) and 48% HBr (5 mL) at 0 °C. The reaction was allowed to warm to RT slowly, and then heated to 60 °C for 5 minutes. The product was extracted with DCM (100 mL X 2). The extractions were combined, dried over sodium sulfate, adsorbed onto silica gel, and purified by MPLC to provide methyl 3-bromo-2-chlorobenzoate. LC-MS (IE, m/z) 251 [M + 1]+
Step C: (3-bromo-2-chlorophenyl methanol: To a solution of methyl 3-bromo-2-chlorobenzoate (1.9 g, 7.6 mmol) in THF (30 mL) was added Super Hydride (23 mL, 23 mmol) at 0 °C. The reaction was allowed to stir for 16 hours. The reaction was diluted with EtOAc, washed with brine, dried over sodium sulfate, and purified by MPLC to provide (3-bromo-2- chlorophenyl)methanol. LC-MS (IE, m/z): 205 [M- 17]+;
Step D: 5-bromo-4-cMoro-2-benzofuran-l(3H -one: To a flask charged with (3-bromo-2- chlorophenyl)methanol (1.1 g, 4.8 mmol) and a stir bar was added thallium trifluoroacetate (2.9 g, 5.3 mmol) and TFA (6 mL). The mixture was allowed to stir at RT for 16 hours. The volatiles were removed under reduced pressure. The residue was pumped under high vacuum for 15 minutes before palladium (II) chloride (0.085 g, 0.48 mmol), magnesium oxide (0.39 g, 9.6 mmol), lithium chloride (0.20 g, 4.8 mmol), and ethanol (30 mL) were added. The mixture was stirred under an atmosphere of carbon mono-oxide until the reaction turned black. The reaction was diluted with DCM. The suspension was filtered through a pad of celite to remove the solids. The filtrate was adsorbed onto silica gel, and purified by MPLC to afford the title compound. Step E: 5-allyl-4-chloro-2-benzofuran-l(3H)-one: To a flask charged with 5-bromo-4-chloro-2- benzofuran-l(3H)-one (190 mg, 0.77 mmol) and a stir bar was added allyl tri-n-butyltin (0.36 mL, 1.2 mmol), PdCl2(dppf)-DCM complex, lithium chloride (0.098 mg, 2.3 mmol), and toluene (5 mL). The flask was fitted with a condensor, purged three times with nitrogen, and heated to reflux for 6 hours. When LC showed complete reaction, the crude material was purified by MPLC to provide 5-allyl-4-cMoro-2-benzofuran-l(3H)-one. LC-MS (IE, m/z): 209 [M+l ]+; Step E: (4-chloro-l-oxo-l -dihydro-2-ben2ofuran-5-vnacetaldehyde: Ozone was bubbled through a solution of 5-allyl-4-chloro-2-benzofuran-l(3H)-one (80 mg, 0.38 mmol) in MeOH at -78 °C until it turned light blue. After excess ozone was removed by bubbling nitrogen through the solution, dimethyl sulfide (0.57 mL, 7.7 mmol) was added into the reaction. The solution was allowed to warm to RT. The crude material was purified by MPLC to provide (4-chloro-l- oxo-l,3-dihydro-2-benzofuran-5-yl)acetaldehyde. LC-MS (IE, m/z): 211 [M+l ]+.
INTERMEDIATE 27
Figure imgf000037_0001
(4-Methyl- 1 -oxo- 1 ,3 -dihydro-2-benzofuran-5-yl)acetaldehvde
Step A: 4-methyl-5-prop-2-en-l-yl-2-benzofuran-l(3H)-one:
To a flask charged with 5-Bromo-4-methyl-2-benzofuran-l(3H)-one (320 mg, 1.41 mmol) and a stir bar was added Allyl tri-n-butyltin (0.655 ml, 2.11 mmol), Pd(PPh3)4 (244 mg, 0.211 mmol), lithium chloride (179 mg, 4.23 mmol), and toluene (15 mL). The reaction was purged with nitrogen 2 times then was heated at reflux for 4 hours. The product was purified by silica gel chromatography to give the title compound.
Step B: (4-methyl-l-oxo-l ,3-dihvdro-2-benzofuran-5-ynacetaldehvde
A solution of the above olefin (220 mg, 1.2 mmol) in MeOH (20 mL) was cooled to -78 °C. To this solution was bubbled ozone until the reaction turned blue. Nitrogen was bubbled through the reaction to drive off excess ozone, followed by addition of DMS (0.870 mL, 11.7 mmol).
The reaction was allowed to warm up to RT. The crude product was purified by flash
chromatography to afford (4-methyl-l-oxo-l,3-dihydro-2-benzofuran-5-yl)acetaldehyde.
Ή-NMR (500 MHz, CDC13) δ ppm 9.78 (s, 1H), 7.75 (d, J= 7.5 Hz, 1H), 7.34 (d, J= 7.5 Hz, 1H), 5.27 (s, 2H), 3.90 (s, 2H), 2.23 (s, 3H).
I
Figure imgf000037_0002
2-(,Methyloxy)-4-(2-oxoethyl)benzonitrile
Step A: 2-(Methyloxy -4-prop-2-en-l-ylbenzonitrile
To a 50 mL flask containing a stir bar were added 2-methoxy-4-bromobenzonitrile (0.30 g, 1.4 mmol), palladium tetrakis (82 mg, 0.071 mmol), allyltri-n-butyltin (0.88 mL, 2.8 mmol), and lithium chloride (0.120 g, 2.83 mmol). The resulting mixture was then dissolved in anhydrous toluene (16 mL); the flask was placed in an oil bath and heated at 130 °C. LC as well as TLC (hexanes/EtOAc =1/0.3) indicated that reaction had gone to completion. The flask was taken out of the oil bath and cooled to room temperature. To the flask was poured EtOAc (40 mL) and the mixture was transferred into a separatory funnel and washed with aqueous NaCl. The organic phase was dried over Na2S04, filtered and concentrated to dryness. It was then dissolved in DCM and absorbed into silica gel. The slica gel was then loaded onto a silica column for separation with the solvent systems of hexanes/EtOAc (1/0.3); this gave 2-(methyloxy)-4-prop-2-en-l- ylbenzonitrile. LC-MS (IE, m z): 174 [M + 1]+.
Step B: 2-(MethyloxyV4-(2-oxoethvDbenzonitrile
To a 25 mL flask containing a stir bar was added compound 2-(methyloxy)-4-prop-2-en-l- ylbenzonitrile (0.15 g, 0.87 mmol) and MeOH (8 mL). The flask was placed in a cold bath of -78 °C. Ozone was bubbled through the flask for about 10 min. followed by addition of dimethyl sulfide (1.5 mL, 24 mmol). The flask was taken out of the cold bath and stirred at room temperature for 1 h; LC indicated completion of the reaction. The reaction mixture was concentrated to dryness to give 2-(methyloxy)-4-(2-oxoethyl)benzonitrile. LC-MS (IE, m/z): 176 [M + 1]+.
INTERMEDIATE 29
Figure imgf000038_0001
2-Fluoro-4-(2-oxoethyl)benzonitrile:
Step A: Methyl (3-fluoro-4-hvdroxyphenyl') acetate
(3-Fluoro-4-hydroxy-phenyl)-acetic acid (25 g, 150 mmol) was dissolved in methanol (100 mL), and thionyl chloride (5 mL) was added dropwise to the solution. The solution was heated to 85 °C for 16 hours. The reaction mixture was allowed to cool and evaporated to dryness in vacuo. The residue was partitioned between ethyl acetate and saturated NaHC03. The organic layer was dried over MgS0 , filtered, and the filtrate was evaporated to dryness under reduced pressure to yield the crude title compound as an off white solid (25.3 g). lU NMR (500 MHz, CDC13): δ 7.07 (d, J= 7.07 Hz, 1H), 7.00-6.97 (m, 2H), 3.74 (s, 3H), 3.73 (s, 2H).
Step B: Methyl (3-fluoro-4-( (trifluoromethyl')sulfonyl]oxy|phenyl)acetate
The crude phenol [methyl (3-fluoro-4-hydroxyphenyl)acetate, 25.3 g] was dissolved in anhydrous dichloromethane (200 mL). 4-Dimethylaminopyridine (1.68 g) was added, followed by triethylamine (23.0 mL, 165 mmol). The solution was then cooled to in a dry ice and acetone bath while under nitrogen. Trifluoromethanesulfonic anhydride (27.9 mL, 165 mmol) was slowly added and the reaction mixture was allowed to warm to ambient temperature. The reaction mixture was then diluted with dichloromethane (200 mL) and washed with water (2 x 100 mL). The organic layer was dried over magnesium sulfate, filtered, and concentrated to dryness under reduced pressure and a cold water bath to yield the crude triflate. LC-MS (IE, m/z): 316 [M+l]+. Step C: Methyl (4-cyano-3 -fluorophenyl) acetate
The crude triflate (43.4 g) was subsequently dissolved in anhydrous dimethylformamide (100 mL). Zinc cyanide (7.2 g, 78 mmol) was added, and the solution was purged thoroughly with nitrogen. Tetrakis(triphenylphosphine) palladium(O) (12 g, 14 mmol) was then added and the reaction mixture was heated to 80 °C for 4h. After allowing cooling to ambient temperature and diluting with water (300 mL), ethyl acetate (500 mL) was added. The combined layers were filtered to remove any solids, the filtrate transferred to a separatory funnel, and the layers separated. The aqueous layer was re-extracted with ethyl acetate (1 x 100 mL), the organic portions were combined and dried over magnesium sulfate. The dry organics were then filtered and evaporated to dryness under reduced pressure and excess dimethylformamide was removed by evaporation in vacuo at 65 °C for 1.5 h to yield the crude title compound (42 g). The crude product was purified through silica gel chromatography (ethyl acetate :hexanes = 2:3) to yield the title nitrile. 1H NMR (500 MHz, CDC13): δ 7.62 (t, J= 6.1 Hz, 1H), 7.23-7.21 (m, 2H), 3.76 (s, 3H), 3.73 (s, 2H). LC-MS (IE, mlz): 194 [M+l]+.
Step D: 2-Fluoro-4-(2-hvdroxyethyl)benzonitrile
LiBH4 (1.94 mL, 3.88 mmol, 2 M in THF) was added to a stirred solution of methyl(4-cyano-3- fiuorophenyl)acetate (0.50 g, 2.59 mmol) in THF (25 ml) at 0 °C. The resulting solution was stirred for 12 h. Water (10 ml) was added, and the resulting solution was extracted with dichloromethane (2 x 50 ml). The combined organic layers were dried over MgS04, filtered, and evaporated under reduced pressure. The residue was purified by column chromatography eluting with EtOAc-Hexanes (7:3→ 1 :1) to give the product as colorless oil. 1H NMR (500 MHz, CDCl3) (5 7.60 (dd, J= 7.4, J= 7.1 Hz, lH ), 7.30 (s, 1H), 7.18 (dd, J= 7.3, J= 9.1 Hz, 1H), 3.95 (t, J= 6.2 Hz, 2H), 2.93 (t, J= 6.3 Hz, 2H).
Step E: 2-Fluoro-4-(2-oxoethyl)benzonitrile:
To a stirred solution of 2-fluoro-4-(2-hydroxyethyl)benzonitrile (0.40 g, 2.4 mmol) in dry CH2C12 (20 mL) at 0 °C was added Dess-Martin periodinane (1.54 g, 3.6 mmol) in one portion. The mixture was stirred for 12 h at rt and quenched with a 1 :1 mixture of saturated Na2S203 (10 mL) and saturated NaHC03 (10 mL). The resulting mixture was diluted with CH2C12 (70 mL) and the layers were separated. The aqueous phase was extracted with CH2C12 (2 χ 50 mL). The combined organic phases were washed with brine, dried (Na2S04), and concentrated in vacuo to give crude aldehyde as colorless oil. The residue was used in the next step without further purification. LC- MS (IE, mlz): 164.1 [M+l]+.
Intermediates described above may be referred to by their number preceded by "I-". For example, Intermediate 1 is shortened to 1-1.
1
Figure imgf000039_0001
6-{2-Οχο-4-[2-(Ί-οχο-1 J-dihydro-2-benzofuran-5-yl)ethyl]piperazin-l-yl)naphthalene-2- carbonitrile: To a 25 mL pear shaped flask charged with 6-(2-Oxopiperazin-l-yl)naphthalene-2- carbonitrile hydrochloride [1-5] (30 mg, 0.10 mmol) and a stir bar was added (l-Oxo-1,3- dihydro-2-benzofuran-5-yl)acetaldehyde [I-l](28 mg, 0.16 mmol), sodium triacetocyborohydride (22 mg, 0.10 mmol), DCM, and a few drops of acetic acid. The mixture was allowed to stir at RT for 16 hours. The title product was purified by mass-directed reverse phase HPLC (AcCN- Water with 0.1% TFA). LC-MS (IE, m/z) 412 [M + 1]+.
Figure imgf000040_0001
6-{4-r(2/. -2-Hydroxy-2-(4-methyl-l-oxo-l,3-dihy
1 - yl I naphthalene-2-carbonitrile : A mixture of 6-(2-Oxopiperazin-l-yl)naphthalene-2- carbonitrile (33 mg, 0.13 mmol) and 4-methyl-5-[(2i?)-oxiran-2-yl]-2-benzofuran-l(3H)-one (38 mg, 0.20 mmol) in EtOH (2 mL) in a 5 mL microwave tube was heated to 148 °C for 1.5 hours. LC showed formation of the title product, which was purified by mass-directed reverse phase HPLC (AcCN- Water with 0.1 % TFA). LC-MS (IE, m/z) 442 [M + 1 ]+.
Figure imgf000040_0002
6- (4- [2-Hvdroxy-2-(6-methyl- 1 -oxo- 1 ,3 -dihydro-2-benzofuran-5 -vDethyl] -2-oxopiperazin- 1 - yl } naphthalene-2-carbonitrile : A mixture of 6-(2-Oxopiperazin-l-yl)naphthalene-2-carbonitrile [1-5] (40 mg, 0.16 mmol) and 6-methyl-5-(oxiran-2-yl)-2-ber^ofuran-l(3H)-one [1-16] (30 mg, 0.16 mmol of the fast eluting isomer) in EtOH (1.5 mL) was heated to 140 °C for 1 hour. The reaction mixture was concentrated to dryness, and dissolved in MeOH, filtered and shot into Mass-directed HPLC for purification to give the title product. LC-MS (IE, m/z) 442 [M + 1]+.
EXAMPLE 4
Figure imgf000041_0001
2- {4-[(2i?)-2-Hvdroxy-2-(4-methyl- 1 -oxo- 1 -dihvdro-2-benzofuran-5-ynethyl]-2-oxopiperazin- l-yllquinoline-6-carbonitrile: A mixture of 2-(2-Oxopiperazin-l-yl)quinoline-6-carbonitrile [I- 2](20 mg, 0.079 mmol) and 4-methyl-5-[(2i?)-oxiran-2-yl]-2-benzofuran-l(3H)-one (30 mg, 0.16 mmol) in EtOH (2 mL) was heated to 140 °C for 1 hour. The reaction mixture was concentrated to dryness, and dissolved in MeOH, filtered and shot into Mass-directed HPLC for purification to give the title product. LC-MS (IE, m/z): 443 [M + 1]+.
Figure imgf000041_0002
3-i4-\(2R )-2-Hvdroxy-2-(4-methyl-l-oxo-1 -dihv(ko-2-benzofuran-5-yl ethyll-2-oxopiperazin- 1 -yl isoquinoline-7-carbonitrile: A mixture of 3-(2-oxopiperazin-l-yl)isoquinoline-7- carbonitrile [1-6] (20 mg, 0.079 mmol) and 4-methyl-5-[(2i?)-oxiran-2-yl]-2-benzofuran-l(3H)- one (30 mg, 0.16 mmol) in EtOH (1 mL) in a 5 mL microwave tube was heated to 145 °C for 1.5 hours. LC showed formation of title product, which was purified by mass-directed reverse phase HPLC (AcCN- Water with 0.1% TFA). LC-MS (IE, m/z): 443 [M + 1 ]+.
Figure imgf000041_0003
7-(4-r(2i?)-2-Hvdroxy-2-(4-methyl-l-oxo-13-
1 -yl)naphthalene-2-carbonitrile: A mixture of 7-(2-oxopiperazin- 1 -yl)naphthalene-2-carbonitrile [1-7] (35 mg, 0.14 mmol) and 4-methyl-5-[(2i?)-oxiran-2-yl]-2-benzofuran-l(3H)-one (40 mg, 0.21 mmol) in EtOH (2 mL) in a 5 mL microwave tube was heated to 145 °C for 2 hours. LC showed formation of the title product, which was purified by silica gel flash chromatography. LC-MS (IE, m/z) 442 [M + 1]+.
Figure imgf000042_0001
2- {4-[(2ifl-2-Hydroxy-2-(4-methyl- 1 -oxo- 1 ,3-dihvdro-2-benzofuran-5-yl')ethyl]-2-oxopiperazin- 1 -yl } - 1 ,3-benzothiazole-5-carbonitrile: A mixture of 2-(2-Oxopiperazin- 1 -yl)- 1 ,3 -benzothiazole- 5-carbonitrile [I-8](25 mg, 0.10 mmol) and 4-methyl-5-[(2i?)-oxiran-2-yl]-2-benzofuran-l(3 )- one (37 mg, 0.19 mmol) in EtOH (2 mL) in a 5 mL microwave tube was heated to 145 °C for 1.5 hours. LC showed formation of the title product, which was purified by silica gel flash chromatography. LC-MS (IE, m/z) 449 [M + 1]+.
Figure imgf000042_0002
6- (4-|Y2i? -2-Hydroxy-2-(4-methyl- 1 -oxo- 1 -dihydro-2-berizofuran-5-yl)ethyl1-2-oxopiperazin- l-yl|thieno[2,3-b1pyridine-2-carbonitrile: A mixture of 4-[(2i?)-2-hydroxy-2-(4-methyl-l-oxo- 1 ,3-dihydro-2-beiizofuran-5-yl)ethyl]piperazin-2-one [I- 10](33 mg, 0.11 mmol), 6- cWorothieno[2,3-b]pyridine-2-carbonitrile [I-13](22 mg, 0.11 mmol), Pd2(dba)3 (21 mg, 0.023 mmol), Xantphos (26 mg, 0.045 mmol), and Cs2C03 (55 mg, 0.17 mmol) in dioxane (2 mL) was sealed in a microwave tube and purged three times with nitrogen. It was heated to 120 °C for 20 minutes in a microwave reactor. LC showed formation of the title product, which was purified by silica gel flash chromatography. LC-MS (IE, m/z) 449 [M + 1]+.
EXAMPLE 9
Figure imgf000043_0001
2-{4-[(2J?)-2-Hydroxy-2-(4-methyl-l-oxo-l,3-dihydro-2-benzofuran-5-yl)ethyl]-2-oxopiperazm
1 -yl ) - 1,3 -benzothiazole-6-carbonitrile
Step A: 2-Chloro-l,3-benzothiazole-6-carbonitrile is commercially available or can be prepared as described in Example 268 of US Patent Publication No. US2009/019862.
Step B: A mixture of 4-[(2i?)-2-hydroxy-2-(4-methyl-l-oxo-l,3-dihydro-2-benzofuran-5- yl)ethyl]piperazin-2-one [I- 10] (30 mg, 0.10 mmol), 2-chloro-l,3-benzothiazole-6-carbonitrile (20 mg, 0.10 mmol), Pd2(dba)3 (19 mg, 0.021 mmol), Xantphos (24 mg, 0.041 mmol), and Cs2C03 (50 mg, 0.16 mmol) in dioxane (2 mL) was sealed in a microwave tube and purged three times with nitrogen. It was heated to 120 °C for 10 minutes in a microwave reactor. LC showed formation of the title product, which was purified by silica gel flash chromatography. LC-MS (IE, m/z): 449 [M + 1]+.
Figure imgf000043_0002
7-{4-[(2i?)-2-Hvdroxy-2-(4-methyl-l-oxo-1 -dihvdro-2-benzofuran-5-vDethyl]-2-oxopiperazin- 1 -yl } quinoline-3 -carbonitrile : A mixture of 7-(2-oxopiperazin-l-yl)quinoline-3 -carbonitrile [1-9] (20 mg, 0.079 mmol) and 4-methyl-5-[(2i?)-oxiran-2-yl]-2-benzofuran-l(3H)-one (30 mg, 0.16 mmol) in EtOH (1.5 mL) in a 5 mL microwave tube was heated to 140 °C for 2 hours. LC showed formation of the title product, which was purified by mass-directed reverse phase HPLC (AcCN-Water with 0.1% TFA). LC-MS (IE, m/z) 443 [M + 1 ]+.
EXAMPLE 11
Figure imgf000044_0001
3-{4-["(2j?)-2-hvdroxy-2-(4-memyl-l-oxo-13-dmvdro-2-benzoruran-5-yl)ethy
1 -yl)quinoline-7-carbonitrile: A mixture of 3-(2-oxopiperazin-l-yl)quinoline-7-carbonitrile [1-4] (17 mg, 0.067 mmol) and 4-methyl-5-[(2i.)-oxiran-2-yl]-2-berizofuran-l(3H)-one (19 mg, 0.10 mmol) in EtOH (1.5 mL) in a 5 mL microwave tube was heated to 140 °C for 1 hour. LC showed formation of the title product, which was purified by mass-directed reverse phase HPLC (AcCN- Water with 0.1% TFA). LC-MS (IE, m/z): 443 [M + 1]+.
Figure imgf000044_0002
2-{4-[(2i.)-2-Hvdroxy-2-(4-memyl-l-oxo-L3-dihvdro-2-benzofuran-5-yl>ethyl]-2-oxopiperazm^ 1 -yl } -7-methylquinoline-6-carbonitrile: A mixture of 7-Methyl-2-(2-oxopiperazin-l- yl)quinoline-6-carbonitrile [I-3](125 mg, 0.47 mmol) and 4-methyl-5-[(2i?)-oxiran-2-yl]-2- benzofuran-l(3H)-one (134 mg, 0.70 mmol) in EtOH (1.5 mL) in a 5 mL microwave tube was heated to 140 °C for 20 minutes. LC showed formation of the desired product, which was purified by mass-directed reverse phase HPLC (AcCN-Water with 0.1% TFA). LC-MS (IE, m/z): 457 [M + 1]+.
Figure imgf000044_0003
3-{4-[(2i?V2-hydroxy-2-(4-methyl-l-oxo-l ,3-dihvdro-2-benzofuran-5-vDethyl1-2-oxopiperazin- l-yl}-8-methylisoquinoline-7-carbonitrile: A mixture of 4-[(2i?)-2-hydroxy-2-(4-methyl-l-oxo- l,3-dihydro-2-benzofuran-5-yl)ethyl]piperazin-2-one (26 mg, 0.089 mmol), 3-Chloro-8- methylisoquinoline-7-carbonitrile [1-12] (18 mg, 0.089 mmol), Pd2(dba)3 (16 mg, 0.017 mmol), Xantphos (21 mg, 0.036 mmol), and CS2CO3 (40 mg, 0.12 mmol) in dioxane (4 mL) was sealed in a microwave tube and purged three times with nitrogen. It was heated to 100 °C for 10 minutes in a microwave reactor. LC showed formation of the desired product, which was purified by silica gel flash chromatography. LC-MS (IE, m/z) 457 [M + 1]+.
Figure imgf000045_0001
6-l4-[(2i?V2-Hvdroxy-2-(4-methyl-l-oxo-l J-dihvdro-2-benzofuran-5-yl)ethyl1-2-oxopiperazin- 1 - vU - 1 -benzothiophene-2-carbonitrile : A mixture of 4-[(2i?)-2-hydroxy-2-(4-methyl- 1 -oxo- 1 ,3- dihydro-2-benzofuran-5-yl)ethyl]piperazin-2-one (50 mg, 0.17 mmol), 6-Bromo-l- benzothiophene-2-carbonitrile [1-11](41 mg, 0.17 mmol), Pd2(dba)3 (32 mg, 0.034 mmol), Xantphos (40 mg, 0.069 mmol), and Cs2C03 (84 mg, 0.26 mmol) in dioxane (1 mL) was sealed in a microwave tube and purged three times with nitrogen. It was heated to 120 °C for 20 minutes in a microwave reactor. LC showed formation of the desired product, which was purified by silica gel flash chromatography. LC-MS (IE, m/z): 448 [M + 1]+.
PLE 15
Figure imgf000045_0002
4-r R)-2-hvdroxy-2-f 4-methyl- 1 -oxo- 1 ,3-dihvdro-2-benzofuran-5-yl)ethyl1- 1 -Γ2- (methylsulfonyl quinolin-6-yl]piperazin-2-one
A mixture of 4-[(2i?)-2-hydroxy-2-(4-methyl-l-oxo-l,3-dihydro-2-benzofuran-5- yl)ethyl]piperazin-2-one (66 mg, 0.23 mmol), 6-Bromo-2-(methylsulfonyl)quinoline [1-14] (65 mg, 0.23 mmol), Pd2(dba)3 (42 mg, 0.045 mmol), Xantphos (53 mg, 0.091 mmol), and Cs2C03 (1 10 mg, 0.34 mmol) in DMF (5 mL) was sealed in a microwave tube and purged three times with nitrogen. It was heated to 120 °C for 10 minutes in a microwave reactor. LC showed formation of the product. The material was purified by silica gel flash chromatography, followed by crystallization to give the title product. LC-MS (IE, m/z): 496 +.
The following Examples in Table 1 can be made from 1-1
Figure imgf000046_0001
and starting material also shown in the Table, using the synthetic procedures described above.
Figure imgf000046_0002
The following Examples in Table 2 can be made from 1-16
Figure imgf000047_0001
and the starting material also shown in the Table, using the synthetic procedures described above.
Figure imgf000047_0002
The following Examples in Table 3 can be made from the intermediate starting materials also shown in the Table, using the synthetic procedures described above.
Figure imgf000048_0001
The following Thallium Flux Assay was performed on the final product compounds in all the Examples. This assay makes use of the ability of thallium to permeate through open ROMK channels and increase the fluorescence of a dye previously loaded into the cells. Under control conditions, cells loaded with dye and exposed to thallium-containing medium display a time- dependent increase in fluorescence, the rate of which depends on number of functional channels. When cells are incubated in the presence of a channel inhibitor, the increase in fluorescence is attenuated in a concentration-dependent manner, and IC50 values of inhibition by compounds can be accurately determined. This assay has been established with cell lines expressing either human, or rat ROMK channels, and operates in 384-well format.
Thallium Flux Assay
Cell Culture Conditions- HEK293 cells stably expressing hROMK (hKirl.l) were grown at 37°C in a 10%CO2 humidified incubator in complete growth media: Dulbecco's Modified Eagle Medium supplemented with non-essential amino acids, Penicillin/Streptomycin/Glutamine, G418 and FBS. At >80% confluency, aspirate the media from the flask and rinse with 10 mL
Calcium/Magnesium-free PBS. Add 5 mL of IX trypsin (prepared in Ca/Mg Free PBS) to T-225 flask and return flask to 37°C/C02 incubator for 2-3 minutes. To dislodge the cell, gently bang the side of the flask with your hand. Triturate the cells completely and then transfer the cells to 25 mL complete media. Centrifuge at 1 ,500 rpm for 6 min followed by resuspension in complete growth media and determine cell concentration. For typical re-seeding, 4E6 cells/T-225 flask will attain >80% confluency in 4 days. Under ideal growth conditions and appropriate tissue culture practices, this cell line is stable for 40-45 passages. FluxOR Kit Components (Invitrogen F10017)
• FluxOR™ Reagent (Component A)
• FluxOR™ Assay Buffer (Component B) - 10X Concentrate
· PowerLoad™ Concentrate (Component C) - 100X Concentrate
• Probenecid (Component D) - Lyophilized sample is kept at -20°C. Water soluble, 100X after solubilization in 1 mL water. Store at 4°C.
• FluxOR™ Chloride-free Buffer (Component E) - 5X Concentrate
• Potassium sulfate (K2S04) Concentrate (Component F) - 125 raM in water. Store at 4°C. · Thallium sulfate (T12S04) Concentrate (Component G) - 50 mM in water. Store at 4°C
• DMSO (dimethyl sulfoxide, Component H) - 1 mL (100%)
Reagent preparation: FluxOR Working Solutions
• 1000X FluxOR™ Reagent: Reconstitute a vial of component A in 100 μΐ DMSO; Mix well; Store 10 μΐ aliquots at -20°C
· 1 X FluxOR™ Assay Buffer: Dilute Component B 10-fold with water; Adjust pH to 7.4 with Hepes/NaOH; Filter and store at 4°C
• Probenecid/ Assay Buffer: 100 mL of IX FluxOR™ Assay Buffer; 1 mL of reconstituted component D; Store at 4°C
• Loading Buffer (per microplate): 10 μΐ 1000X FluxOR™ Reagent; 100 μΐ component C; 10 mL Probenecid/ Assay Buffer
• Compound Buffer (per microplate): 20 mL Probenecid/ Assay Buffer; 0.3 mM ouabain (10 mM ouabain in water can be stored in amber bottle/aluminum foil at room temperature); Test compound
• IX FluxOR™Chloride-Free Buffer: Prepare IX working solution in water. Can be stored at room temperature
• Stimulant Buffer (prepared at 5X final concentration in IX FluxOR™Chloride-Free Buffer): 7.5 mM Thallium sulfate and 0.75 mM Potassium sulfate (to give a final assay concentration of 3 mM Thallium/ 0.3 mM Potassium). Store at 4°C when not in use. If kept sterile, this solution is good for months.
Assay protocol- The ROMK channel functional thallium flux assay is performed in 384 wells, using the FLIPR-Tetra instrument. HEK-hKirl.l cells are seeded in Poly-D-Lysine microplates and kept in a 37°C-10%CO2 incubator overnight. On the day of the experiment, the growth media is replaced with the FluxOR™ reagent loading buffer and incubated, protected from light, at ambient temperature (23-25°C) for 90 min. The loading buffer is replaced with assay buffer ± test compound followed by 30 min incubation at ambient temperature, where the Thallium/Potassium stimulant is added to the microplate.
Step Protocol 1. Seed HEK-hKirl.l cells (50 μΐ at 20,000 cells/well) in 384-well PDL coated Microplates
2. Allow cells to adhere overnight in humidified 37°C/10%CO2 incubator
3. Completely remove cell growth media from microplate and replace with 25 μΐ loading buffer
4. Incubate Microplate at room temperature, protected form light, for 90 min
5. Remove loading buffer and replace with 25 μΐ lx Assay Buffer ± test compound.
6. Incubate microplate at room temperature, protected form light, for 30 min
7. At FLIPR-Tetra 384: Add stimulant (Thallium/Potassium) solution to microplate and monitor fluorescence. Excitation = 400 ran, Emission = 460 & 580 nm. Collect data for ~ 10 min.
Data Calculation- The fluorescence intensity of wells containing 3 μΜ of a standard control ROMK inhibitor of the present invention is used to define the ROMK-sensitive component of thallium flux. Fluorescence in the presence of test compounds is normalized to control values to provide % fluorescence change. IC50 values represent the concentration of compound that inhibits 50% of the ROMK thallium flux signal.
Assay Standard- Normally, a control compound is included to support that the assay is giving consistent results compared to previous measurements, although the control is not required to obtain the results for the test compounds. The control can be any compound of Formula I of the present invention, preferably with an IC50 potency of less than 1 μΜ in this assay. Alternatively, the control could be another compound (outside the scope of Formula I) that has an IC50 potency in this assay of less than 1 μΜ.
Representative examples of data collected for compounds of the present invention using the Thallium Flux Assay are shown in Table 4 below. All of the final product compounds in Examples 1-15 were tested in the Thallium Flux Assay and had IC50 results of 0.5 μΜ or less.
TABLE 4
Figure imgf000050_0001
12 0.08
13 0.15
14 0.16
15 0.22
While the invention has been described with reference to certain particular embodiments thereof, numerous alternative embodiments will be apparent to those skilled in the art from the teachings described herein. Recitation or depiction of a specific compound in the claims (i.e., a species) without a specific stereoconfiguration designation, or with such a designation for less than all chiral centers, is intended to encompass the racemate, racemic mixtures, each individual enantiomer, a diastereoisomeric mixture and each individual diastereomer of the compound where such forms are possible due to the presence of one or more asymmetric centers. All patents, patent applications and publications cited herein are incorporated by reference in their entirety.

Claims

WHAT IS CLAIMED IS:
1. A compound having structural Formula I:
Figure imgf000052_0001
and the pharmaceutically acceptable salts thereof wherein:
Figure imgf000052_0002
wherein the dashed bonds represent the optional presence of -Cl-2alkanediyl- which, when present, forms a 5 to 6 membered carbocyclic ring which is fused to the aryl ring;
Figure imgf000052_0003
Rl is -H, -CH3, -CF3, -CHF2, -C¾F or -CH2OH;
R2 is -H, oxo (=0) or -Ci-6alkyl;
R4 is -H, -OH, oxo or -Ci -6alkyl;
provided that when R.4 is -OH or oxo, then R2 is not oxo;
R3 and R5 are each independently -H or -Ci-6alkyl; provided that R.3 is absent when R.2 is oxo, and R5 is absent when R4 is oxo;
Rla is -H, halo or -Ci -3 lkyl;
Rib is -H, halo, -Ci_3alkyl, -0-Ci-3alkyl or -COOCi.3alkyl;
one of R2a and R2b 1S -CN and the other is -H or -Ci-3alkyl; one of R2c and R2d 1S -S02-Ci-3alkyl and the other is -H or -Ci-3alkyl;
Xl, X2, χ3 and χ are each independently CH or N, provided that only one of Xl , χ2, χ3 0r χ4 is N;
X5, χ6 and X7 are each independently CH or N, provided that only one of X5, χ6 0r X7 is N; and
Ra and Rb are each independently -H, halo, -Ci_3alkyl, -0-Ci-3alkyl or -COOCi.3alkyl.
2. The compound of claim 1 or a pharmaceutically acceptable salt thereof having structural Formula II:
Figure imgf000053_0001
3. The compound of claim 1 or a pharmaceutically acceptable salt thereof wherein Z\ is zl-a.
4. The compound of claim 3 or a pharmaceutically acceptable salt thereof wherein R2, R3 and R.5 are each -H, and R4 is -H, -OH or oxo.
5. The compound of claim 1 or a pharmaceutically acceptable salt thereof wherein Z\ is zl-b.
6. The compound of claim 5 or a pharmaceutically acceptable salt thereof wherein R2, R3 and R5 are each -H, and R4 is -H, -OH or oxo.
7. The compound of claim 1 or a pharmaceutically acceptable salt thereof wherein Zl is zl-c.
8. The compound of claim 7 or a pharmaceutically acceptable salt thereof wherein the dashed bonds in zl-c represent -Ci-2alkanediyl-.
9. The compound of claim 7 or a pharmaceutically acceptable salt thereof wherein the dashed bonds in zl-c are absent and -Cl-2alkanediyl- is not present.
10. The compound of claim 1 which is
6- {2-Oxo-4- [2-( 1 -oxo- 1 ,3 -dihydro-2-benzofuran-5-yl)ethyl]piperazin- 1 -yl} naphthalene-2- carbonitrile; 6-{4-[(2J?)-2-Hydroxy-2-(4-methyl-l-oxo-l,3-dihydro-2-berizoruran-5-yl)ethyl]
1 -yl } naphthalene-2-carbonitrile;
6-{4-[2-Hydroxy-2-(6-methyl-l-oxo-l,3-dihydro-2-benzor an-5-yl)ethyl]-2-oxopiperazin-l- yl } naphthalene-2-carbonitrile ;
2-{4-[(2i-)-2-Hydroxy-2-(4-methyl-l-oxo-l,3-dihydro-2-benzor½an-5-yl)emyl]-2-oxopiperazin-
1 -yl} -1 ,3-benzothiazole-5-carbonitrile; or
2- {4-[(2i?)-2-Hydroxy-2-(4-me1hyl- 1 -oxo- 1 ,3 -dihydro
1 -yl} -7-methylquinoline-6-carbonitrile;
or a pharmaceutically acceptable salt thereof.
11. A pharmaceutical composition comprised of a compound of claim 1 and a pharmaceutically acceptable carrier.
12. The pharmaceutical composition of claim 11 further comprising one or more additional active agent selected from losartan, valsartan, candesartan, olmesartan, telmesartan, eprosartan, irbesartan, azilsartan, hydrochlorothiazide, amlodipine, alacepril, benazepril, captopril, ceronapril, cilazapril, delapril, enalapril, enalaprilat, fosinopril, imidapril, lisinopril, moveltipril, perindopril, quinapril, ramipril, spirapril, temocapril, trandolapril, amiloride, spironolactone, epleranone, triamterene or acetazolamide or a pharmaceutically acceptable salt thereof.
13. A method for inhibiting ROMK comprising administering a compound of claim 1 in a ROMK- inhibitory effective amount to a patient in need thereof.
14. A method for causing diueresis, natriuresis or both, comprising administering a compound of claim 1 in a therapeutically effective amount to a patient in need thereof.
15. A method for the treatment or prophylaxis of one or more disorders selected from hypertension, acute heart failure, chronic heart failure, pulmonary arterial hypertension, cardiovascular disease, diabetes mellitus, diabetes insipidus, post-operative volume overload, endothelial dysfunction, diastolic dysfunction, systolic dysfunction, stable and unstable angina pectoris, thromboses, restenosis, myocardial infarction, stroke, cardiac insufficiency, pulmonary hypertonia, atherosclerosis, hepatic cirrhosis, ascitis, pre-eclampsia, cerebral edema, nephropathy, glomerulonephritis, nephrotic syndrome, acute and chronic kidney insufficiency, acute tubular necrosis, hypercalcemia, idiopathic edema, Dent's disease, Meniere's disease, edematous states, glaucoma, and benign intracranial hypertension comprising administering a compound of claim 1 in a therapeutically or prophylactically effective amount as appropriate, to a patient in need thereof.
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US20140288088A1 (en) 2014-09-25
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US8999991B2 (en) 2015-04-07

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