WO2013020996A1 - Composition for controlled ovarian stimulation - Google Patents

Composition for controlled ovarian stimulation Download PDF

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Publication number
WO2013020996A1
WO2013020996A1 PCT/EP2012/065507 EP2012065507W WO2013020996A1 WO 2013020996 A1 WO2013020996 A1 WO 2013020996A1 EP 2012065507 W EP2012065507 W EP 2012065507W WO 2013020996 A1 WO2013020996 A1 WO 2013020996A1
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WIPO (PCT)
Prior art keywords
fsh
dose
patient
pmol
product
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PCT/EP2012/065507
Other languages
French (fr)
Inventor
Joan-Carles Arce
Original Assignee
Ferring Bv
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Family has litigation
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Application filed by Ferring Bv filed Critical Ferring Bv
Priority to JP2014524372A priority Critical patent/JP6161609B2/en
Priority to IL300380A priority patent/IL300380A/en
Priority to KR1020197036505A priority patent/KR20190140098A/en
Priority to DK12753675.3T priority patent/DK2741763T3/en
Priority to SI201230438T priority patent/SI2741763T1/en
Priority to RS20160080A priority patent/RS54561B1/en
Priority to PL19213649T priority patent/PL3646881T3/en
Priority to BR122020001644-2A priority patent/BR122020001644B1/en
Priority to US14/237,697 priority patent/US9694052B2/en
Priority to CN201280038831.7A priority patent/CN103732243A/en
Priority to EP21207717.6A priority patent/EP4005588B1/en
Priority to KR1020207029774A priority patent/KR20200121918A/en
Priority to AU2012293647A priority patent/AU2012293647B2/en
Priority to PL14184409T priority patent/PL2821080T3/en
Priority to EP12753675.3A priority patent/EP2741763B1/en
Priority to EP19164277.6A priority patent/EP3566712B1/en
Priority to EP19213649.7A priority patent/EP3646881B1/en
Priority to BR112014002884A priority patent/BR112014002884A2/en
Priority to KR1020147005938A priority patent/KR102014469B1/en
Priority to PL19164277T priority patent/PL3566712T3/en
Priority to EP14184409.2A priority patent/EP2821080B1/en
Priority to KR1020217037512A priority patent/KR102499969B1/en
Priority to PL18167552T priority patent/PL3395357T3/en
Priority to EP18167552.1A priority patent/EP3395357B1/en
Priority to KR1020217023662A priority patent/KR102353144B1/en
Priority to RU2014102924A priority patent/RU2613324C3/en
Priority to EP20186935.1A priority patent/EP3756681B1/en
Priority to PL17154031T priority patent/PL3195875T3/en
Priority to NZ620369A priority patent/NZ620369B2/en
Priority to EP17154031.3A priority patent/EP3195875B1/en
Priority to PL12753675T priority patent/PL2741763T3/en
Priority to KR1020237004855A priority patent/KR20230024442A/en
Priority to KR1020197024335A priority patent/KR102111514B1/en
Priority to CA2844282A priority patent/CA2844282A1/en
Priority to EP23208228.9A priority patent/EP4306174A3/en
Priority to MX2014001489A priority patent/MX348981B/en
Priority to ES12753675.3T priority patent/ES2562648T3/en
Publication of WO2013020996A1 publication Critical patent/WO2013020996A1/en
Priority to IL230696A priority patent/IL230696B2/en
Priority to ZA2014/00952A priority patent/ZA201400952B/en
Priority to HK14111597.8A priority patent/HK1198005A1/en
Priority to HRP20160145TT priority patent/HRP20160145T1/en
Priority to SM201600038T priority patent/SMT201600038B/en
Priority to AU2016273925A priority patent/AU2016273925B2/en
Priority to US15/637,962 priority patent/US10624953B2/en
Priority to AU2018203222A priority patent/AU2018203222B2/en
Priority to US16/851,260 priority patent/US11291708B2/en
Priority to US17/709,305 priority patent/US20220370567A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors

Definitions

  • composition for controlled ovarian stimulation Composition for controlled ovarian stimulation
  • the present invention relates to compositions and pharmaceutical products for the treatment of infertility.
  • ART Assisted reproductive technology
  • IVF in vitro fertilisation
  • COS controlled ovarian stimulation
  • FSH follicle stimulating hormone
  • LH luteinising hormone
  • the pharmaceutical compositions generally used for COS include recombinant follicle stimulating hormone (rFSH), urinary derived FSH, recombinant FSH + LH preparations, urinary derived menotrophin [human menopausal gonadotrophin (hMG)] and highly purified human menopausal gonadotrophin (HP-hMG).
  • IVF can be associated with a risk of ovarian hyperstimulation syndrome (OHSS), which can be life threatening in severe cases.
  • OHSS ovarian hyperstimulation syndrome
  • the ability to predict the response potential of women to controlled ovarian stimulation may allow the development of individualised COS protocols. This could, for example, reduce the risk of OHSS in women predicted to have an excessive response to stimulation, and/or improve pregnancy outcomes in women classed as poor responders.
  • the serum concentration of anti-Mullerian hormone (AMH) is now established as a reliable marker of ovarian reserve. Decreasing levels of AMH are correlated with reduced ovarian response to gonadotrophins during COS. Further, high levels of AMH are a good predictor of excessive ovarian response, and an indicator of risk of OHSS.
  • FSH is naturally secreted by the anterior pituitary gland and functions to support follicular development and ovulation.
  • FSH comprises a 92 amino acid alpha sub-unit, also common to the other glycoprotein hormones LH and CG, and a 1 1 1 amino acid beta sub-unit unique to FSH that confers the biological specificity of the hormone (Pierce and Parsons, 1981 ). Each sub-unit is post translationally modified by the addition of complex carbohydrate residues.
  • Both subunits carry 2 sites for N-linked glycan attachment, the alpha sub-unit at amino acids 52 and 78 and the beta sub-unit at amino acid residues 7 and 24 (Rathnam and Saxena, 1975, Saxena and Rathnam, 1976).
  • FSH is thus glycosylated to about 30% by mass (Dias and Van Roey. 2001. Fox et al. 2001 ).
  • FSH purified from post-menopausal human urine has been used for many years in infertility treatment; both to promote ovulation in natural reproduction and to provide oocytes for assisted reproduction technologies.
  • the currently approved recombinant FSH (rFSH) products for ovarian stimulation such as follitropin alfa (GONAL-F, Merck Serono / EMD Serono) and follitropin beta (PUREGON / FOLLISTIM, MSD / Schering-Plough), are derived from a Chinese Hamster Ovary (CHO) cell line.
  • follitropin alfa GONAL-F, Merck Serono / EMD Serono
  • PUREGON / FOLLISTIM MSD / Schering-Plough
  • FSH FSH
  • isoforms exhibit identical amino acid sequences but differ in the extent to which they are post-translationally modified; particular isoforms are characterised by heterogeneity of the carbohydrate branch structures and differing amounts of sialic acid (a terminal sugar) incorporation, both of which appear to influence the specific isoform bioactivity.
  • Glycosylation of natural FSH is highly complex.
  • the glycans in naturally derived pituitary FSH can contain a wide range of structures that can include combinations of mono-, bi- , tri- and tetra-antennary glycans (Pierce and Parsons, 1981. Ryan et al., 1987. Baenziger and Green, 1988).
  • the glycans can carry further modifications: core fucosylation, bisecting glucosamine, chains extended with acetyl lactosamine, partial or complete sialylation, sialylation with a2,3 and a2,6 linkages, and sulphated galactosamine substituted for galactose (Dalpathado et al., 2006).
  • the glycosylation of recombinant FSH products reflects the range of glycosyl- transferases present in the host cell line.
  • the commercially available rFSH products are derived from engineered Chinese hamster ovary cells (CHO cells).
  • the range of glycan modifications in CHO cell derived rFSH are more limited than those found on the natural products.
  • Examples of the reduced glycan heterogeneity found in CHO cell derived rFSH include a lack of bisecting glucosamine and a reduced content of core fucosylation and acetyl lactosamine extensions (Hard et al., 1990).
  • CHO cells are only able to add sialic acid using the a2,3 linkage (Kagawa et al, 1988, Takeuchi et al, 1988, Svensson et al., 1990); CHO cell derived rFSH only includes a2,3-linked sialic acid and does not include a2,6-linked sialic acid.
  • CHO cell derived FSH is different from naturally produced FSH (e.g. human Pituitary/ serum/ urinary FSH) which contains glycans with a mixture of a2,3 and a2,6-linked sialic acid, with a predominance of the former.
  • FSH e.g. human Pituitary/ serum/ urinary FSH
  • the commercially available recombinant FSH preparation differs in the amounts of FSH with an isoelectric point (pi) of below 4 (considered the acidic isoforms) when compared to pituitary, serum or post-menopausal urine FSH (Ulloa-Aguirre et al. 1995).
  • the amount of acidic isoforms in the urinary preparations was much higher as compared to the CHO cell derived recombinant products, Gonal-f (Merck Serono) and Puregon (Schering Plough) (Andersen et al. 2004).
  • the circulatory life-time of FSH has been documented for materials from a variety of sources. Some of these materials have been fractionated on the basis of overall molecular charge, as characterised by their pi, in which more acid equates to a higher negative charge.
  • the major contributor to overall molecular charge is the total sialic content of each FSH molecule.
  • rFSH Organon
  • urine-derived FSH has a higher sialic acid content
  • the corresponding plasma clearance rates in the rat are 0.34 and 0.14 ml/min (Ulloa-Aguirre et al. 2003).
  • recombinant proteins expressed using the CHO system will differ from their natural counterparts in their type of terminal sialic acid linkages. This is an important consideration in the production of biologicals for pharmaceutical use since the carbohydrate moieties may contribute to the pharmacological attributes of the molecule.
  • Recombinant FSH with a mixture of both a2,3 and a2,6-linked sialic acid was made by engineering a human cell line to express both rFSH and a2,3 sialyltransferase.
  • the expressed product is highly acidic and carries a mix of both a2,3- and a2,6-linked sialic acids; the latter provided by the endogenous sialyl transferase activity.
  • the rFSH product disclosed in International Patent Application No. PCT/GB2009/000978 contains branched glycan moieties.
  • FSH comprises glycans (attached to the FSH glycoproteins) and these glycans may contain a wide variety of structures.
  • branching can occur with the result that the glycan may have 1 , 2, 3, 4 or more terminal sugar residues or "antennae”; glycans with 1 , 2, 3 or 4 terminal sugar residues or "antennae” are referred to respectively as mono-antennary, di-antennary, tri- antennary or tetra-antennary structures.
  • Glycans may have sialylation presence on mono- antennary and/or di-antennary and/or tri-antennary and/or tetra-antennary structures.
  • a mono-sialylated glycan structure carries one sialic acid residue; a di-sialylated glycan structure carries two sialic acid residues; a tri- sialylated glycan structure carries three sialic acid residues; and a tetra-sialylated glycan structure carries four sialic acid residues.
  • X% mono-sialylated refers to the number of glycan structures on FSH which are mono-, di, tri or tetra sialylated (respectively), expressed as a percentage (X%) of the total number of glycan structures on the FSH which are sialylated in any way (carry sialic acid).
  • the phrase "30 - 36% tri-sialylated glycan structures" means that, of the total number of glycan structures on the FSH which carry sialic acid residues (that is, are sialylated), 30 to 36% of these glycan structures are tri sialylated (carry three sialic acid residues).
  • FSH having a specific amount of tetra- sialylated glycan structures is markedly more potent then recombinant FSH products which are currently on the market.
  • the amino acid sequence of the applicant's products is the native sequence and is identical to natural human FSH and existing CHO-derived rFSH products.
  • human derived recombinant FSH products i.e. recombinant FSH produced or expressed in a human cell line e.g. made by engineering a human cell line
  • a human cell line e.g. made by engineering a human cell line
  • a specific amount of tetra-sialylated glycan structures may be particularly effective when utilised in (e.g. individualised) COS protocols.
  • a product e.g. a pharmaceutical composition
  • FSH follicle stimulating hormone
  • a patient e.g. a patient having serum AMH level of 0.05 pmol/L or above, for example 0.5 pmol/L or above
  • the product comprises a dose of, or a dose equivalent to, 1-24 ⁇ g, for example 2-24 ⁇ g, for example 2 to 15 ⁇ g, human derived recombinant FSH.
  • the product comprises a dose of, or a dose equivalent to, 4.5 to 12.5 ⁇ g, for example 5 to 12.5 ⁇ g, for example 6 to 12.5 ⁇ g, for example 6.3 to 10.5 ⁇ g, human derived recombinant FSH.
  • a product e.g. a pharmaceutical composition
  • FSH follicle stimulating hormone
  • the product comprises a (e.g. daily) dose of, or dose equivalent to, 9 to 14 ⁇ g, for example 1 1 to 13 ⁇ g, for example 12 ⁇ g human derived recombinant FSH.
  • FSH is a recombinant FSH ("rFSH" or "recFSH”).
  • FSH is a human cell line derived recombinant FSH.
  • the dose provides an effective response while minimising risk of OHSS.
  • the treatment of infertility comprising a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of ⁇ 15 pmol/L (e.g. 0.05 pmol/L to 14.9 pmol/L).
  • a product e.g. a pharmaceutical composition
  • follicle stimulating hormone for use in the treatment of infertility in a patient having serum AMH level of ⁇ 15 pmol/L
  • the product comprises a (e.g. daily) dose of, or dose equivalent to, 5 to 12.5 ⁇ g, for example 6 to 10.5 ⁇ g human derived recombinant FSH.
  • FSH follicle stimulating hormone
  • the FSH is a recombinant FSH ("rFSH" or "recFSH”
  • the FSH is a human cell line derived recombinant FSH.
  • the dose provides an effective response while minimising risk of OHSS.
  • the treatment of infertility comprising a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of ⁇ 15 pmol/L.
  • the product is for use in the treatment of infertility in a patient having serum AMH level of 15 to 24.9 pmol/L, and the product is for administration at a (e.g.
  • the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of 15 to 24.9 pmol/L.
  • the product is for use in the treatment of infertility in a patient having serum AMH level of 25 to 34.9 pmol/L, and the product is for administration at a (e.g.
  • the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of 25 to 34.9 pmol/L.
  • the product is for use in the treatment of infertility in a patient having serum AMH level of ⁇ 35 pmol/L, and the product is for administration at a (e.g.
  • the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of > 35 pmol/L.
  • the doses above may be for treatment of infertility in the patient's (subject's) first stimulation protocol. It will be appreciated that for further stimulation cycles, the doses may be adjusted according to actual ovarian response in the first cycle.
  • a product e.g. a pharmaceutical composition
  • FSH follicle stimulating hormone
  • the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.09 to 0.19 ⁇ g (for example 0.09 to 0.17 ⁇ g) human derived recombinant FSH per kg bodyweight of the patient.
  • the treatment of infertility comprises a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of ⁇ 15 pmol/L.
  • the product is for use in the treatment of infertility in a patient having serum AMH level of 15 to 24.9 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.14 to 0.19 ⁇ g human derived recombinant FSH (preferably 0.15 to 0.16 ⁇ g human derived recombinant FSH) per kg bodyweight of the patient.
  • the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of 15 to 24.9 pmol/L.
  • the product is for use in the treatment of infertility in a patient having serum AMH level of 25 to 34.9 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.1 1 to 0.14 ⁇ g human derived recombinant FSH (preferably 0.12 to 0.13 ⁇ g human derived recombinant FSH) per kg bodyweight of the patient.
  • the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of 25 to 34.9 pmol/L.
  • the product is for use in the treatment of infertility in a patient having serum AMH level of ⁇ 35 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.10 to 0.1 1 ⁇ g human derived recombinant FSH per kg bodyweight of the patient.
  • the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of > 35 pmol/L.
  • the FSH is a recombinant FSH ("rFSH" or "recFSH").
  • the FSH is a human cell line derived recombinant FSH. The doses provide an effective response while minimising risk of OHSS.
  • the doses above may be for treatment of infertility in the patient's (subject's) first stimulation protocol. It will be appreciated that for further stimulation cycles, the doses may be adjusted according to actual ovarian response in the first cycle.
  • a product e.g. a pharmaceutical composition
  • follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH level of ⁇ 15 pmol/L
  • the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.15 to 0.21 ⁇ g, (for example 0.19 to 0.21 ⁇ g) human derived recombinant FSH per kg bodyweight of the patient.
  • the treatment of infertility comprises a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of ⁇ 15 pmol/L.
  • these doses may be readily converted to treat patients with dosing according to their BMI, using conversions well known in the art.
  • the product may be for use in the treatment of infertility in a patient having serum AMH of 5.0-14.9 pmol/L, wherein the product comprises a dose of, or dose equivalent to, 6 to 18 ⁇ g, for example 8 to 1 1 ⁇ g, for example 8.5 to 10.2 ⁇ g human derived recombinant FSH.
  • the product may be for use in the treatment of infertility in a patient having serum AMH 15.0-29.9 pmol/L, wherein the product comprises a dose of, or a dose equivalent to, 4.8 to 15 ⁇ g, for example 6 to 9 ⁇ g, for example 6.8 to 8.5 ⁇ g human derived recombinant FSH.
  • the product may be for use in the treatment of infertility in a patient having serum AMH 30-44.9 pmol/L, wherein the product comprises a dose of, or a dose equivalent to, 3.6 to 12 ⁇ g, for example 4 to 7 ⁇ g, for example 5.1 to 6.8 ⁇ g human derived recombinant FSH.
  • the product may be for use in the treatment of infertility in a patient having serum AMH 45 pmol/L or greater, wherein the product comprises a dose of, or a dose equivalent to, 2 to 9 ⁇ g, for example 2.4 to 9 ⁇ g (for example 3.4 to 5.1 ⁇ g) or 2 to 5 ⁇ g human derived recombinant FSH.
  • the product may comprise follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH of 5 pmol/L or less, wherein the product comprises a dose of, or a dose equivalent to 7.2 to 24 ⁇ g, for example 10 to 15 ⁇ g for example 10.2 to 13.6 ⁇ g, human derived recombinant FSH.
  • the product may be for use in the treatment of infertility in a patient wherein the product comprises a dose of, or dose equivalent to, 4.8 to 18 ⁇ g, for example 6 to 1 1 ⁇ g, for example 6.8 to 10.2 ⁇ g human derived recombinant FSH.
  • the FSH is a recombinant FSH ("rFSH" or "recFSH").
  • the FSH is a human cell line derived recombinant FSH.
  • the rFSH (e.g. human cell line derived recombinant FSH) includes a2,3- and a2,6- sialylation.
  • the FSH (rFSH) for use according to the invention may have 1 % to 99% of the total sialylation being a2,3-sialylation.
  • the FSH (rFSH) according to the invention may have 1 % to 99% of the total sialylation being a2,6-sialylation.
  • 50 to 70% for example 60 to 69%, for example about 65%, of the total sialylation is a2,3-sialylation.
  • Preferably 25 to 50%, for example 30 to 50 %, for example 31 to 38%, for example about 35%, of the total sialylation is a2,6- sialylation.
  • the rFSH (e.g. human cell line derived recombinant FSH) includes mono-, di- , tri- and tetra-sialylated glycan structures, wherein 15-24%, for example 17-23% of the sialylated glycan structures are tetrasialylated glycan structures (e.g. as shown by WAX analysis of charged glycans, as set out in the Examples below).
  • the FSH comprises glycans (attached to the FSH glycoproteins). It is well known that glycans in FSH may contain a wide variety of structures. These may include combinations of mono, bi, tri and tetra-antennary glycans.
  • X% of the sialylated glycan structures are tetrasialylated glycan structures refers to the number of glycan structures on the FSH which are tetra sialylated, i.e. carry four sialic acid residues, expressed as a percentage (X%) of the total number of glycan structures on the FSH which are sialylated in any way (carry sialic acid).
  • the phrase "15-24% of the sialylated glycan structures are tetrasialylated glycan structures" means that, of the total number of glycan structures on FSH which carry sialic acid residues (that is, are sialylated), 15 to 24% of these glycan structures are tetra sialylated (carry four sialic acid residues).
  • the rFSH may be present as a single isoform or as a mixture of isoforms.
  • the serum level of AMH may be determined (e.g. measured) by any method known in the art.
  • the serum AMH level is measured using the AMH Gen-ll enzyme linked immunosorbent assay, a kit (Beckman Coulter, Inc., Webster, Texas). This assay can detect can detect AMH concentrations greater than 0.57 pmol/L with a minimum limit of quantitation of 1.1 pmol/L. Other assays may be used.
  • the product e.g. pharmaceutical composition
  • the (daily) dose may be an initial dose (i.e. it may be reduced, increased, or maintained during the treatment).
  • the product may be for (daily) administration of FSH starting on day one of treatment and continuing for seven to thirteen days, for example nine to thirteen days, for example 10 to 13 days, for example 10 to 1 1 days.
  • the product e.g. pharmaceutical composition
  • the product may be for administration 12 to 16, e.g. 13 to 15, e.g. 14 days after administration of (e.g. after initiation of administration of, e.g. after initiation of daily administration of) a GnRH agonist (e.g. Synarel, Lupron, Decapeptyl).
  • the product e.g. pharmaceutical composition
  • the product may be for administration with a GnRH agonist.
  • the product e.g.
  • the pharmaceutical composition may be for administration prior to administration of a GnRH antagonist (e.g. ganirelix, cetrorelix), for example for administration five or six days prior to administration of a GnRH antagonist.
  • the product e.g. pharmaceutical composition
  • the product may be for administration with a GnRH antagonist.
  • the product e.g. pharmaceutical composition
  • the product may be for dosing at frequencies more (or less) than daily, in which case the relevant doses will be equivalent to the (daily) doses specified herein.
  • treatment of infertility includes treatment of infertility by controlled ovarian stimulation (COS) or methods which include a step or stage of controlled ovarian stimulation (COS), for example Intra Uterine Insemination (IUI), in vitro fertilisation (IVF), or intracytoplasmic sperm injection (ICSI).
  • COS controlled ovarian stimulation
  • IUI Intra Uterine Insemination
  • IVF in vitro fertilisation
  • ICSI intracytoplasmic sperm injection
  • treatment of infertility includes treatment of infertility by ovulation induction (Ol) or by methods which include a step or stage of ovulation induction (01).
  • treatment of infertility includes treatment of infertility in a subject having tubal or unexplained infertility, including treatment of infertility in a subject having endometriosis, for example stage I or stage II endometriosis, and/or in a subject having anovulatory infertility, for example WHO type II anovulatory infertility, and/or in a subject with a partner with male factor infertility.
  • the product (or composition) may be for (use in) the treatment of infertility (and/or for controlled ovarian stimulation) in a subject having endometriosis, for example in a subject having stage I or stage II endometriosis, as defined by The American Society for Reproductive Medicine (ASRM) classification system for the various stages of endometriosis, (stage IV most severe; stage I least severe) [American Society for Reproductive Medicine. Revised American Society for Reproductive Medicine classification of endometriosis: 1996. Fertil Steril 1997; 67,817 821 .].
  • ASRM American Society for Reproductive Medicine
  • the product (composition) may be for (use in) the treatment of infertility (and/or for controlled ovarian stimulation) in a subject having normal serum FSH level of 1 to 16 IU/L, for example 1 to 12 IU/L, in the early follicular phase.
  • the product (composition) may be for (use in) the treatment of infertility (and/or for controlled ovarian stimulation) in a subject aged 18 to 42 years, for example 25 to 37 years.
  • the product may be for (use in) the treatment of infertility (and/or for controlled ovarian stimulation) in a subject having BMI >1 and BMI ⁇ 35 kg/m 2 , for example a subject having BMI >18 and BMI ⁇ 25 kg/m 2 , for example a subject having BMI >20 and BMI ⁇ 25 kg/m 2 .
  • the rFSH may preferably include 27 - 33%, for example 30 - 32%, tri-sialylated glycan structures.
  • the rFSH may preferably include 24 - 33%, for example 26 - 30%, di-sialylated glycan structures.
  • the rFSH may preferably include 12 - 21 %, for example 15 - 17%, mono- sialylated glycan structures.
  • the rFSH preferably includes mono-sialylated, di- sialylated, tri- sialylated and tetra- sialylated glycan structures with relative amounts as follows: 15 to 17% mono-sialylated; 26 - 30% di-sialylated; 27 - 33% (e.g. 29 to 32%, e.g 30-32%, e.g 30 to 31 %) tri-sialylated and 17 - 23 % tetra-sialylated (e.g. as shown by WAX analysis of charged glycans, as set out in the Examples).
  • the rFSH may include from 0 to 7%, for example 0.1 to 7%, for example 3 to 6%, for example 5 to 6%, neutral sialylated structures.
  • the FSH comprises glycans (attached to the FSH glycoproteins).
  • X% mono-sialylated refers to the number of glycan structures on FSH which are mono-, di, tri or tetra sialylated (respectively), expressed as a percentage (X%) of the total number of glycan structures on the FSH which are sialylated in any way (carry sialic acid).
  • the phrase "27 - 33% tri-sialylated glycan structures” means that, of the total number of glycan structures on FSH which carry sialic acid residues (that is, are sialylated), 27 to 33% of these glycan structures are tri sialylated (carry three sialic acid residues).
  • the rFSH may have a sialic acid content [expressed in terms of a ratio of moles of sialic acid to moles of protein] of 6 mol/mol or greater, for example between 6 mol/mol and 15 mol/mol, e.g between 8 mol/mol and 14 mol/mol, for example between 10 mol/mol and 14 mol/mol, e.g between 1 1 mol/mol and 14 mol/mol, e.g between 12 mol/mol and 14 mol/mol, e.g. between 12 mol/mol and 13 mol/mol.
  • the rFSH may be produced or expressed in a human cell line.
  • the FSH (rFSH) for use according to the invention may have 1 % to 99% of the total sialylation being a2,3-sialylation.
  • the rFSH may have 10% or more of the total sialylation being a2,3-sialylation.
  • 20, 30, 40, 50, 60, 70, 80 or 90% or more of the total sialylation may be a2,3-sialylation.
  • the rFSH may preferably include a2,3-sialylation in an amount which is from 50 to 70% of the total sialylation, for example from 60 to 69% of the total sialylation, for example from 63 to 67%, for example around 65% of the total sialylation.
  • the FSH (rFSH) for use according to the invention may have 1 % to 99% of the total sialylation being a2,6- sialylation.
  • the rFSH (or rFSH preparation) of the invention may have 5% or more, for example 5% to 99%, of the total sialylation being a2,6-sialylation.
  • the rFSH may have 50% or less of the total sialylation being a2,6-sialylation.
  • the rFSH may preferably include a2,6- sialylation in an amount which is from 25 to 50% of the total sialylation, for example from 30 to 50% of the total sialylation, for example from 31 to 38%, for example around 35% of the total sialylation.
  • sialylation it is meant the amount of sialic residues present on the FSH carbohydrate structures.
  • a2,3-sialylation means sialylation at the 2,3 position (as is well known in the art) and a2,6 sialylation at the 2,6 position (also well known in the art).
  • % of the total sialylation may be a 2,3 sialylation” refers to the % of the total number of sialic acid residues present in the FSH which are sialylated in the 2,3 position.
  • the term "% of the total sialylation being a2,6-sialylation” refers to the % of the total number of sialic acid residues present in the FSH which are sialylated in the 2,6 position.
  • the rFSH may have a sialic acid content (amount of sialylation per FSH molecule) of (based on the mass of protein, rather than the mass of protein plus carbohydrate) of 6% or greater (e.g. between 6% and 15%, e.g. between 7% and 13%, e.g. between 8% and 12%, e.g. between 1 1 % and 15%, e.g. between 12% and 14%) by mass.
  • sialic acid content amount of sialylation per FSH molecule
  • the rFSH may be rFSH or a rFSH preparation in which 16 % or fewer (e.g. 0.1 to 16%) of the glycans comprise (e.g. carry) bisecting N-acetylglucosamine (bisecting GlcNAc or bisGlcNAc).
  • the rFSH (or rFSH preparation) is an rFSH or rFSH preparation in which 8 to 14.5% of the glycans comprise (e.g. carry) a bisecting N-acetylglucosamine (bisecting GlcNAc or bisGlcNAc).
  • FSH comprises glycans attached to the FSH glycoproteins. It will also be understood that 100% of the glycans refers to or means all of the glycans attached to the FSH glycoproteins.
  • the terminology "8 to 14.5% of the glycans comprise (carry) bisecting N-acetylglucosamine” means that 8 to 14.5% of the total number of glycans attached to the FSH glycoproteins include/carry bisecting N-acetylglucosamine; "16% or fewer of the glycans comprise (carry) bisecting N-acetylglucosamine” means that 16 % or fewer of the total number of glycans attached to the FSH glycoproteins include/carry bisecting N- acetylglucosamine, and so on.
  • recombinant FSH rFSH preparations; rFSH compositions
  • 16% or fewer (e.g. 8 to 14.5%) of the glycans comprised in the FSH glycoproteins carry bisecting GlcNac may have advantageous pharmacokinetic properties. It is believed the advantageous properties may arise because the amount of glycans which carry bisecting GlcNac is similar to that in the human urinary derived product Bravelle, which is rather less than that of other recombinant FSH preparations such as those disclosed in WO2012/017058.
  • the rFSH may be an rFSH or rFSH preparation in which 20% or more of the glycans comprise (e.g. carry) N-Acetylgalactosamine (GalNAc), for example in which 20% or more of the glycans comprise (e.g. carry) a terminal GalNAc.
  • the rFSH (or rFSH preparation) is an FSH or FSH preparation in which the 40 to 55%, for example 42% to 52%, of the glycans comprise (e.g. carry) GalNAc.
  • the rFSH (or rFSH preparation) is an FSH or FSH preparation in which the 40 to 55%, for example 42% to 52%, of the glycans comprise (e.g. carry) terminal GalNAc.
  • FSH comprises glycans attached to the FSH glycoproteins. It will also be understood that 100% of the glycans refers to or means all of the glycans attached to the FSH glycoproteins.
  • the terminology "wherein 20% or more of the glycans comprise (e.g. carry) GalNAc” means that 20% or more of the total number of glycans attached to the FSH glycoproteins include/carry N-Acetylgalactosamine (GalNAc); "40 to 55%, for example 42% to 52%, of the glycans comprise (e.g. carry) terminal GalNAc” means that 40 to 55 %, for example 42% to 52%, of the total number of glycans attached to the FSH glycoproteins include/carry terminal GalNAc, and so on.
  • rFSH is distinguished over other approved products because of the sugar composition: it includes, or may include, a specific amount of GalNac. This may be linked to tetrasialylation and potency because the 2,6- sialylation is associated with GalNac.
  • the present applicants have developed an rFSH product which includes specific characteristics (2,6- linker sites, GalNac) which provide rFSH with high degree of sialylation, which appears to lead to improved potency in vivo.
  • the rFSH (or rFSH preparation) may have 16 to 24% of the glycans comprising (e.g. terminal) 1 fucose-lewis, for example 16.5 to 18% of the glycans comprising (e.g. terminal) 1 fucose-lewis.
  • the rFSH (or rFSH preparation) may have 1 .5 to 4.5%, for example 2 to 4%, for example 3.7%, of the glycans comprising (e.g. terminal) 2 fucose -lewis.
  • the content of fucose-lewis may have an effect on potency.
  • the rFSH may be produced or expressed in a human cell line, for example a Per.C6 cell line, a HEK293 cell line, a HT1080 cell line etc.. This may simplify (and render more efficient) the production method because manipulation and control of e.g. the cell growth medium to retain sialylation may be less critical than with known processes.
  • the method may also be more efficient because there is little basic rFSH produced compared to production of known rFSH products; more acidic rFSH is produced and separation/removal of basic FSH is less problematic.
  • the rFSH may be produced or expressed in a PER.C6® cell line, a PER.C6® derived cell line or a modified PER.C6® cell line.
  • rFSH which is produced or expressed in a human cell line (e.g. PER.C6® cell line, HEK293 cell line, HT1080 cell line etc.) will include some a2,6-linked sialic acids (a2,6 sialylation) provided by endogenous sialyl transferase activity [of the cell line] and will include some a2,3-linked sialic acids (a2,3 sialylation) provided by endogenous sialyl transferase activity.
  • the cell line may be modified using a2,3- sialyltransferase.
  • the cell line may be modified using a2,6-sialyltransferase.
  • the rFSH may include a2,6-linked sialic acids (a2,6 sialylation) provided by endogenous sialyl transferase activity [of the cell line].
  • a2,6 sialylation a2,6 sialylation
  • endogenous sialyl transferase activity of the cell line.
  • human derived recombinant FSH means recombinant FSH which is produced or expressed in a human cell line (e.g. recombinant FSH made by engineering a human cell line).
  • the rFSH may be produced using a2,3- and/or a2,6-sialyltransferase.
  • rFSH is produced using a2,3- sialyltransferase.
  • the rFSH may include a2,6-linked sialic acids (a2,6 sialylation) provided by endogenous sialyl transferase activity.
  • the product may be a pharmaceutical composition.
  • the pharmaceutical composition is for the treatment of infertility.
  • the treatment of infertility may comprise assisted reproductive technologies (ART), ovulation induction or intrauterine insemination (IUI).
  • ART assisted reproductive technologies
  • IUI intrauterine insemination
  • the pharmaceutical composition may be used, for example, in medical indications where known FSH preparations are used.
  • the product or composition can be formulated into well-known compositions for any route of drug administration, e.g. oral, rectal, parenteral, transdermal (e.g. patch technology), intravenous, intramuscular, subcutaneous, intrasusternal, intravaginal, intraperitoneal, local (powders, ointments or drops) or as a buccal or nasal spray.
  • route of drug administration e.g. oral, rectal, parenteral, transdermal (e.g. patch technology), intravenous, intramuscular, subcutaneous, intrasusternal, intravaginal, intraperitoneal, local (powders, ointments or drops) or as a buccal or nasal spray.
  • a typical composition comprises a pharmaceutically acceptable carrier, such as aqueous solution, non toxic excipients, including salts and preservatives, buffers and the like, as described in Remington's Pharmaceutical Sciences fifteenth edition (Matt Publishing Company, 1975), at pages 1405 to 1412 and 1461 - 87, and the national formulary XIV fourteenth edition (American Pharmaceutical Association, 1975), among others.
  • a pharmaceutically acceptable carrier such as aqueous solution, non toxic excipients, including salts and preservatives, buffers and the like, as described in Remington's Pharmaceutical Sciences fifteenth edition (Matt Publishing Company, 1975), at pages 1405 to 1412 and 1461 - 87, and the national formulary XIV fourteenth edition (American Pharmaceutical Association, 1975), among others.
  • aqueous and non-aqueous pharmaceutical carriers, diluents, solvents or vehicles examples include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), carboxymethylcellulose and suitable mixtures thereof, vegetable oils (such as olive oil), and injectible organic esters such as ethyl oleate.
  • the compositions of the present invention also can contain additives such as but not limited to preservatives, wetting agents, emulsifying agents, surfactants and dispersing agents.
  • Antibacterial and antifungal agents can be included to prevent growth of microbes and includes, for example, m-cresol, benzyl alcohol, paraben, chlorobutanol, phenol, sorbic acid, and the like. If a preservative is included, benzyl alcohol, phenol and/or m-cresol are preferred; however, the preservative is by no means limited to these examples. Furthermore, it may be desirable to include isotonic agents such as sugars, sodium chloride, and the like.
  • the product or composition may further comprise a salt comprising a pharmaceutically acceptable alkali metal cation selected from the group consisting of Na + - or K + - salts, or a combination thereof. Preferably the salt is a Na+- salt, for example NaCI or Na 2 S0 4 .
  • the product or composition comprises recombinant FSH and one or more of
  • Polysorbate 20 L-methionine, phenol, disodium sulphate and sodium phosphate buffer.
  • FSH FSH
  • other active ingredients if present
  • This can be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility.
  • the rate of absorption of FSH then depends upon its rate of dissolution which, in turn, can depend upon crystal size and crystalline form.
  • delayed absorption of a parenterally administered FSH combination form is accomplished by dissolving or suspending the FSH combination in an oil vehicle.
  • injectable depot forms can be made by forming microencapsule matrices of the FSH (and other agents, if present) in biodegradable polymers such as polylactide-polyglycolide.
  • the rate of FSH release can be controlled.
  • biodegradable polymers include polyvinylpyrrolidone, poly(orthoesters), poly(anhydrides) etc.
  • Depot injectable formulations are also prepared by entrapping the FSH in liposomes or microemulsions which are compatible with body tissues.
  • Injectable formulations can be sterilized, for example, by filtration through a bacterial- retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium just prior to use.
  • Injectable formulations can be supplied in any suitable container, e.g. vial, pre-filled syringe, injection cartridges, and the like.
  • the product or composition may be formulated for single use or for multiple use
  • the single use or multiple use formulated product or composition may further comprise a salt comprising a pharmaceutically acceptable alkali metal cation selected from the group consisting of Na + - or K + - salts, or a combination thereof.
  • the salt is a Na+- salt, for example NaCI or Na 2 S0 4 .
  • the product or composition may be included in a container such as a vial, prefilled cartridge (e.g. for single administration or multiple use) or an injection device such as a "pen” for e.g. administration of multiple doses .
  • the product or composition may be a formulation (e.g. injectable formulation) including FSH (optionally with hCG, LH, LH activity etc.)
  • the LH activity may originate from LH or human chorionic gonadotropin, hCG. If there is more than one active ingredient (i.e. FSH and e.g. hCG or LH) these may be suitable for administration separately or together. If administered separately, administration can be sequential.
  • the product can be supplied in any appropriate package.
  • a product can include a number of containers (e.g. pre- filled syringes or vials) containing either FSH or hCG, or a combination (or combination) of both FSH and hCG.
  • the hCG may be recombinant hCG or urinary hCG.
  • the product includes a number of containers (e.g. pre-filled syringes or vials) containing FSH, e.g. recombinant FSH, each container may include the same amount of FSH.
  • One or more containers may include different amounts of FSH.
  • the syringes or vials may be packaged in a blister package or other means to maintain sterility. Any product can optionally contain instructions for using the FSH (and e.g. hCG if present) formulations.
  • the pH and exact concentration of the various components of the pharmaceutical composition are adjusted in accordance with routine practice in this field.
  • compositions of the invention are supplied as compositions for parenteral administration.
  • General methods for the preparation of the parenteral formulations are known in the art and are described in REMINGTON; THE SCIENCE AND PRACTICE OF PHARMACY, supra, at pages 780-820.
  • the parenteral compositions can be supplied in liquid formulation or as a solid which will be mixed with a sterile injectable medium just prior to administration.
  • the parenteral compositions are supplied in dosage unit form for ease of administration and uniformity of dosage.
  • a method of treatment of infertility comprising: (a) measuring the serum AMH level of a subject; and (b) administration to the subject a dose of, or a dose equivalent to, 1-24 ⁇ g, for example 2-24 ⁇ g, for example 2 to 15 ⁇ g, human derived recombinant FSH.
  • the dose is, or is equivalent to, 4.5 to 12.5 ⁇ g, for example 5 to 12.5 ⁇ g, for example 6 to 12.5 ⁇ g, for example 6.3 to 12 ⁇ g, human derived recombinant FSH.
  • a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject; and (b) administering a (e.g. daily) dose of, or dose equivalent to, 9 to 14 ⁇ g, for example 1 1 to 13 ⁇ g, for example 12 ⁇ g human derived recombinant follicle stimulating hormone (FSH) to a (the) subject having serum AMH level of ⁇ 15 pmol/L (e.g. 0.05 pmol/L to 14.9 pmol/L).
  • FSH is a recombinant FSH ("rFSH" or "recFSH").
  • the FSH is a human cell line derived recombinant FSH.
  • the dose provides an effective response while minimising risk of OHSS.
  • a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject; and (b) administering a (e.g. daily) dose of, or dose equivalent to, 5 to 12.5 ⁇ g human derived recombinant follicle stimulating hormone (FSH) to a (the) subject having serum AMH level of ⁇ 15 pmol/L.
  • the (e.g. daily) dose may be, or be equivalent to, 6 to 10 ⁇ g human derived recombinant follicle stimulating hormone (FSH).
  • the FSH is a recombinant FSH ("rFSH" or "recFSH").
  • the FSH is a human cell line derived recombinant FSH.
  • the dose provides an effective response while minimising risk of OHSS.
  • the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 5 to 12 ⁇ g, for example 7 to 12 ⁇ g, for example 8.7 to 10 ⁇ g, human derived recombinant FSH (preferably 9 to 10 ⁇ g human derived recombinant FSH) to a (the) subject having serum AMH level of 15 to 24.9 pmol/L
  • a step of administering a e.g.
  • the method includes a step of administering a (e.g.
  • a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject; and (b) administering a (e.g. daily) dose of, or dose equivalent to, 0.09 to 0.19 ⁇ g (for example 0.09 to 0.17 ⁇ g) human derived recombinant FSH per kg bodyweight of the subject, wherein the subject has serum AMH level of ⁇ 15 pmol/L.
  • the FSH is a recombinant FSH ("rFSH" or "recFSH”).
  • the FSH is a human cell line derived recombinant FSH.
  • the dose provides an effective response while minimising risk of OHSS.
  • the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 0.14 to 0.19 ⁇ g human derived recombinant FSH (preferably 0.15 to 0.16 ⁇ g human derived recombinant FSH) per kg bodyweight of the subject, the subject having serum AMH level of 15 to 24.9 pmol/L .
  • the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 0.1 1 to 0.14 ⁇ g human derived recombinant FSH (preferably 0.12 to 0.13 ⁇ g human derived recombinant FSH) per kg bodyweight of the subject, the subject having serum AMH level of 25 to 34.9 pmol/L.
  • the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 0.10 to 0.1 1 ⁇ g human derived recombinant FSH per kg bodyweight of the subject, the subject having serum AMH level of ⁇ 35 pmol/L.
  • the FSH is a recombinant FSH ("rFSH" or "recFSH").
  • rFSH recombinant FSH
  • recFSH human cell line derived recombinant FSH.
  • a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject; and (b) administering a (e.g. daily) dose of, or dose equivalent to, 0.15 to 0.21 ⁇ g (for example 0.19 to 0.21 ⁇ g) human derived recombinant FSH per kg bodyweight of the subject, wherein the subject has serum AMH level of ⁇ 15 pmol/L.
  • the administration preferably comprises a daily dose of, or a daily dose equivalent to, the amount of FSH defined above and in the claims.
  • the (daily) dose may be an initial dose (it may be reduced, increased, or maintained during the treatment).
  • the method may be a method of treatment of infertility in the patient's (subject's) first stimulation protocol. It will be appreciated that for further stimulation cycles, the doses may be adjusted according to actual ovarian response in the first cycle.
  • a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject;
  • the subject has serum AMH level of 15 to 24.9 pmol/L, administering to the subject a dose of, or dose equivalent to, 0.14 to 0.19 ⁇ g human derived recombinant FSH (preferably 0.15 to 0.16 ⁇ g human derived recombinant FSH) per kg bodyweight of the subject; or
  • the subject has serum AMH level of 25 to 34.9 pmol/L pmol/L, administering to the subject a dose of, or dose equivalent to, 0.1 1 to 0.14 ⁇ g human derived recombinant FSH (preferably 0.12 to 0.13 ⁇ g human derived recombinant FSH) per kg bodyweight of the subject; or
  • the subject has serum AMH level of ⁇ 35 pmol/L pmol/L, administering to the subject a dose of, or dose equivalent to, 0.10 to 0.1 1 ⁇ g human derived recombinant FSH per kg bodyweight of the subject.
  • a dose of, or dose equivalent to, 6 to 18 ⁇ g, for example 8 to 1 1 ⁇ g, for example 8.5 to 10.2 ⁇ g human derived recombinant FSH may be administered.
  • a dose of, or a dose equivalent to, 4.8 to 15 ⁇ g, for example 6 to 9 ⁇ g, for example 6.8 to 8.5 ⁇ g human derived recombinant FSH may be administered.
  • a dose of, or a dose equivalent to, 3.6 to 12 ⁇ g, for example 4 to 7 ⁇ g, for example 5.1 to 6.8 ⁇ g human derived recombinant FSH may be administered.
  • a dose of, or a dose equivalent to, 2 to 9 ⁇ g, for example 2.4 to 9 ⁇ g (for example 3.4 to 5.1 ⁇ g) or 2 to 5 ⁇ g human derived recombinant FSH may be administered.
  • human derived recombinant FSH may be administered.
  • a dose of, or dose equivalent to, 4.8 to 18 ⁇ g, for example 6 to 1 1 ⁇ g, for example 6.8 to 10.2 ⁇ g human derived recombinant FSH is administered.
  • the FSH is a recombinant FSH ("rFSH" or "recFSH").
  • the FSH is a human cell line derived recombinant FSH.
  • the administration preferably comprises a daily dose of, or a daily dose equivalent to, the amount of FSH defined above and in the claims.
  • the (daily) dose may be an initial dose (it may be reduced, increased, or maintained during the treatment.
  • Figure 1 shows a plasmid map of the pFSHalpha/beta expression vector
  • Figure 2 shows the a2,3-sialyltransferase (ST3GAL4) expression vector
  • Figure 3 shows the a2,6-sialyltransferase (ST6GAL1 ) expression vector
  • Figure 4 shows % abundance sialic acid distribution of examples of recombinant FSH produced by PER.C6® cells stably expressing FSH after engineering with a2,3- sialyltransferase;
  • Figure 5 shows % abundance of glycan charge distribution of examples of recombinant FSH produced by PER.C6® cells stably expressing FSH after engineering with a2,3- sialyltransferase;
  • Figure 6 shows a comparison of concentration of inhibin-B following administration of 225IU Gonal f (bottom line, dotted line) and 225 IU of the Example (top line, full line) of Invention;
  • Fig 7 shows the effect of body weight on oocytes retrieved in the low AMH treatment group (Example 10, 10A);
  • Fig 8 shows the effect of Body weight on oocytes retrieved in the high AMH treatment group
  • the coding region of the gene for the FSH alpha polypeptide was used to according to Fiddes and Goodman. (1981 ).
  • the sequence is banked as AH007338 and at the time of construction there were no other variants of this protein sequence.
  • the sequence is referred herein as SEQ ID NO:1.
  • the coding region of the gene for FSH beta polypeptide was used according to Keene et al (1989).
  • the sequence is banked as NM_000510 and at the time of construction there were no other variants of this protein sequence.
  • the sequence is referred herein as SEQ ID NO: 2
  • a2,3-Sialyltransferase The coding region of the gene for beta-galactoside alpha-2,3- sialyltransferase 4 (a2,3-sialyltransferase, ST3GAL4) was used according to Kitagawa and Paulson (1994). The sequence is banked as L23767 and referred herein as SEQ ID NO: 3. a2,6-Sialyltransferase - The coding region of the gene for beta-galactosamide alpha- 2,6-sialyltransferase 1 (a2,6-sialyltransferase, ST6GAL1 ) was used according to Grundmann et al. (1990). The sequence is banked as NM_003032 and referred herein as SEQ ID NO: 4.
  • FSH alpha polypeptide AH007338, SEQ ID NO: 1
  • FSH beta polypeptide NM_003032, SEQ ID NO: 2
  • FSHa-fw and FSHa-rev and FSHb-fw and FSHb-rec were amplified by PCR using the primer combinations FSHa-fw and FSHa-rev and FSHb-fw and FSHb-rec respectively.
  • FSHa-rev 5'-GGATGGCTAGCTTAAGATTTGTGATAATAAC-3' SEQ ID NO:10
  • the resulting amplified FSH beta DNA was digested with the restriction enzymes Asc ⁇ and Hpa ⁇ and inserted into the Asc ⁇ and Hpa ⁇ sites on the CMV driven mammalian expression vector carrying a neomycin selection marker.
  • the FSH alpha DNA was digested with SamHI and Nhe ⁇ and inserted into the sites SamHI and Nhe ⁇ on the expression vector already containing the FSH beta polypeptide DNA.
  • the vector DNA was used to transform the DH5a strain of E.coli. Colonies were picked for amplification. Colonies containing the vector containing both FSH alpha and beta were selected for sequencing and all contained the correct sequences according to SEQ ID NO: 1 and SEQ ID NO: 2. Plasmid pFSH A+B#17 was selected for transfection ( Figure 1 ).
  • SEQ ID NO: 3 was amplified by PCR using the primer combination 2,3STfw and 2,3STrev.
  • the resulting amplified ST3 DNA was digested with the restriction enzymes BamH ⁇ and AflW and inserted into the BamH ⁇ and AflW sites on the CMV driven mammalian expression vector carrying a hygromycin resistance marker.
  • the vector was amplified as previously described and sequenced.
  • Clone pST3#1 ( Figure 2) contained the correct sequence according SEQ ID NO: 3 and was selected for transfection.
  • NM_003032, SEQ ID NO: 4 was amplified by PCR using the primer combination 2,6STfw and 2,6STrev.
  • the resulting amplified ST6 DNA was digested with the restriction enzymes BamH ⁇ and AflW and inserted into the BamH ⁇ and AflW sites on the CMV driven mammalian expression vector carrying a hygromycin resistance marker.
  • the vector was amplified as previously described and sequenced.
  • Clone pST6#1 1 ( Figure 3) contained the correct sequence according SEQ ID NO: 4 and was selected for transfection.
  • Example 4 Stable expression of pFSH ⁇ + ⁇ in PER.C6® cells. Transfection isolation and screening of clones.
  • PER.C6®clones producing FSH were generated by expressing both polypeptide chains of FSH from a single plasmid (see Example 1 ).
  • a liposome based transfection agent with the pFSH ⁇ + ⁇ construct Stable clones were selected in VPRO supplemented with 10% FCS and containing G418. Three weeks after transfection G418 resistant clones grew out. Clones were selected for isolation. The isolated clones were cultured in selection medium until 70-80% confluent. Supernatants were assayed for FSH protein content using an FSH selective ELISA and pharmacological activity at the FSH receptor in cloned cell line, using a cAMP accumulation assay. Clones expressing functional protein were progressed for culture expansion to 24 well, 6 well and T80 flasks.
  • PER.C6® clones producing highly sialylated FSH were generated by expressing a2,3 sialyltransferase from separate plasmids (Example 2) in PER.C6® cells already expressing both polypeptide chains of FSH (from Example 4). Clones produced from PER.C6® cells as set out in Example 4 were selected for their characteristics including productivity, good growth profile, production of functional protein, and produced FSH which included some sialylation. Stable clones were generated as previously described in Example 4. Clones were isolated, expanded and assayed. The a2,3-sialyltransferase clones were adapted to serum free media and suspension conditions.
  • FSH from a2,3- clone was prepared using a using a modification of the method described by Lowry et al. (1976).
  • the cell lines were adapted to a serum- free medium, i.e., Excell 525 (JRH Biosciences).
  • the cells were first cultured to form a 70%-90% confluent monolayer in a T80 culture flask. On passage the cells were re-suspended in the serum free medium, Excell 525 + 4 rtiM L-Glutamine, to a cell density of 0.3x10 6 cells/ml.
  • a 25 ml cell suspension was put in a 250 ml shaker flask and shaken at 100 rpm at 37°C at 5% CO 2 .
  • the cells After reaching a cell density of > IxlO 6 cells/ml, the cells were sub-cultured to a cell density of 0.2 or 0.3x10 6 cells/ml and further cultured in shaker flasks at 37°C, 5% C0 2 and 100 rpm.
  • FSH FSH
  • the cells were transferred to a serum- free production medium, i.e., VPRO (JRH Biosciences), which supports the growth of PER.C6®cells to very high cell densities (usually > 10 7 cells/ml in a batch culture).
  • the cells were first cultured to > 1 xlO 6 cells/ml in Excell 525, then spun down for 5 min at 1000 rpm and subsequently suspended in VPRO medium + 6 mM L-glutamine to a density of 1x10 6 cells/ml. The cells were then cultured in a shaker flask for 7-10 days at 37°C, 5% CO2 and 100 rpm. During this period, the cells grew to a density of > 10 7 cells/ml. The culture medium was harvested after the cell viability started to decline. The cells were spun down for 5 min at 1000 rpm and the supernatant was used for the quantification and purification of FSH. The concentration of FSH was determined using ELISA (DRG EIA 1288).
  • N-Glycans were released from the samples using PNGase F under denaturative conditions and then labelled with 2-aminobenzamide. Released glycan forms were then separated and analysed by Weak Anion Exchange (WAX) column for determination of charge distribution. Labelled glycans treated with 2,3,6,8 sialidase for determination of total sialic acid and 2,3 sialidase for determination of 2,3 sialic acid, were further analyzed by wax column.
  • WAX Weak Anion Exchange
  • the relative percentages of the charged glycans were calculated from structures present in the undigested and digested glycan pools and are shown in Figure 4 (for 8 samples). These were found to be in the ranges 50% - 70% (e.g. about 60% or 65%) for a2,3 sialylation and 28 to 50%, generally 30 to 35% (e.g. about 31 % or 35%), for a2,6 sialylation.
  • Example 8 Quantification of relative amounts mono, di, tri and tetra sialylated glycan structures
  • N Glycans were released from the samples using PNGase F under denaturative conditions and then were labeled with 2-aminobenzamide.
  • Glycans were released from the samples using PNGase F under denaturative conditions and then labeled with 2- aminobenzamide. Released glycan forms were then separated and analysed by Weak Anion Exchange (WAX) column for determination of sialylation distribution. The relative amounts of neutral, mono-sialylated, di-sialylated, tri-sialylated and tetra-sialylated structures are shown in Figure 5 (for the 8 samples shown in Fig 4).
  • WAX Weak Anion Exchange
  • the rFSH includes neutral , mono-sialylated, di- sialylated, tri- sialylated and tetra- sialylated glycan structures with relative amounts as follows: neutral 5-6 %; 15-17% mono- sialylated; 26-30% di-sialylated; 30-32% tri-sialylated and 17-23 % tetra-sialylated.
  • N-Glycans were released from the samples using PNGase F under denaturative conditions and then labelled with 2-aminobenzamide. Released glycan forms were then separated and analysed by Weak Anion Exchange (WAX) column for determination of charge distribution. Labelled glycans treated with 2,3,6,8 sialidase for determination of total sialic acid and 2,3 sialidase for determination of 2,3 sialic acid, were further analyzed by wax column (see Example 8). The analysis allows calculation of a2,6 sialic acid.
  • WAX Weak Anion Exchange
  • the relative percentages of the charged glycans were calculated from structures present in the undigested and digested glycan pools and are shown in the following Table. These were found to be in the ranges 25 to 50%, generally 30 to 35% for a2,6 sialylation.
  • the glycans were analyzed by MALDI-MS The relative amounts of alpha 2,6-sialic acid and the terminal residues are shown in the following table, together with those for Gonal F (CHO cell derived recombinant FSH) and Bravelle (human urinary FSH).
  • Gonal F CHO cell derived recombinant FSH
  • Bravelle human urinary FSH
  • the amount of GalNac in the FSH of the invention varies between about 44.9 and 51 %, averaging about 47.1 %.
  • the amount of bisecting GlcNac in the FSH of the invention varies between 8.7 and 13.9%, averaging approximately at 10.9%.
  • the amount of 1 Fucose Lewis in the FSH of the invention varies between 16.1 and 23.3%, averaging approximately at 19%.
  • Example 9 A multiple dose study investigating the safety, tolerability, pharmacokinetics, pharmacodynamics, and immunogenicity of FE 999049 in comparison to GONAL-F.
  • FE 999049 and GONAL-F were safe and generally well tolerated as assessed by Adverse Events (AEs), vital signs, ECG, clinical laboratory measurements, and physical examination. No serious adverse event or death occurred during the study.
  • AEs Adverse Events
  • vital signs vital signs
  • ECG ECG
  • clinical laboratory measurements ECG
  • physical examination No serious adverse event or death occurred during the study.
  • Example 9 demonstrates that FSH having a specific amount (17-23%) of tetra-sialylated glycan structures and e.g. specific amounts of a2,3 sialylation and a2,6 sialylation is markedly more potent then recombinant FSH products which are currently on the market.
  • Example 10 A multiple dose study investigating FE 999049 in comparison to GONAL-F.
  • the following describes a randomised, controlled, assessor-blind, parallel groups, multinational, multicentre trial assessing the dose-response relationship of FE 999049 in patients undergoing controlled ovarian stimulation for in vitro fertilisation (IVF) / intracytoplasmic sperm injection (ICSI).
  • IVF in vitro fertilisation
  • ICSI intracytoplasmic sperm injection
  • the trial was designed as a dose-response trial with number of oocytes retrieved as the primary endpoint. Secondary endpoints will explore the qualitative and quantitative impact of different doses of FE 999049 with regard to endocrine profile, follicular development, oocyte fertilisation, embryo quality and treatment efficiency (i.e. total gonadotropin consumption and duration of stimulation).
  • the trial is designed to evaluate the efficacy of FE 999049 to establish pregnancy when used in controlled ovarian stimulation for IVF/ICSI cycles.
  • Subjects were assessed within 3 months prior to randomisation for compliance with the inclusion and exclusion criteria, including an anti-Mijllerian hormone (AMH) assessment to increase homogeneity of the trial population in relation to ovarian response and minimise the number of potential poor and hyper-responders to the FE 999049 doses and GONAL-F dose used in the trial.
  • AMH assessment was measured using the AMH Gen-ll enzyme linked immunosorbent assay kit (Beckman Coulter, Inc., Webster, Texas). This assay can detect AMH concentrations greater than 0.57 pmol/L with a minimum limit of quantitation of 1 .1 pmol/L.
  • Gonal-F is filled by mass (FbM) at FDA request; referring to ⁇ g dose is therefore appropriate.
  • the Gonal -F label indicates 600 IU/44 ⁇ g, which indicates that 150 IU is 1 1 ⁇ g.
  • the FE999049 doses are presented by protein content (pg) rather than biological activity. Thus the doses of FE999049 were 5.2pg (90 IU), 6.9pg (120 IU), 8.6pg (150 IU), 10.3pg (180 IU) or 12.1 pg (210 IU).
  • the subject and dose distribution is set out as follows (data are number of subjects):
  • the daily dose level of FE 999049 or GONAL-F is fixed throughout the entire stimulation period. During stimulation, subjects are monitored on stimulation day 1 , 4 and 6 and hereafter at least every second day. When 3 follicles of >15 mm are observed, visits are performed daily. Subjects are treated with FE 999049 or GONAL-F for a maximum of 16 days.
  • a GnRH antagonist (ganirelix acetate,
  • ORGALUTRAN MSD / Schering-Plough
  • MSD may be initiated on stimulation day 6 at a daily dose of 0.25 mg and continued throughout the stimulation period.
  • Triggering of final follicular maturation is done on the day when >3 follicles with a diameter >17 mm are observed. If there are ⁇ 25 follicles with a diameter >12 mm, 250 pg recombinant hCG (choriogonadotropin alfa, OVITRELLE, Merck Serono / EMD Serono) is administered.
  • hCG choriogonadotropin alfa, OVITRELLE, Merck Serono / EMD Serono
  • GnRH agonist triptorelin acetate, DECAPEPTYL / GONAPEPTYL, Ferring Pharmaceuticals
  • >35 follicles with a diameter >12 mm the treatment is cancelled.
  • poor ovarian response defined as ⁇ 3 follicles with a diameter >10 mm observed on stimulation day 10, the cycle could be cancelled.
  • Oocyte retrieval takes place 36h ( ⁇ 2h) after triggering of final follicular maturation and the oocytes inseminated by IVF and/or ICSI. Fertilisation and embryo development are assessed from oocyte retrieval to the day of transfer. For subjects who underwent triggering of final follicular maturation with hCG, one blastocyst of the best quality available is transferred on day 5 after oocyte retrieval while remaining blastocysts are frozen. For subjects who undergo triggering of final follicular maturation with GnRH agonist, no embryo transfer takes place in the fresh cycle and blastocysts are instead frozen on day 5.
  • Vaginal progesterone tablets (LUTINUS, Ferring Pharmaceuticals) 100 mg 3 times daily are provided for luteal phase support from the day after oocyte retrieval until the day of the clinical pregnancy visit.
  • a ⁇ test is performed 13-15 days after embryo transfer and clinical pregnancy will be confirmed by transvaginal ultrasound (TVU) 5-6 weeks after embryo transfer.
  • the number of oocytes retrieved (primary endpoint) is shown in the following Table.
  • the serum FSH concentrations after exposure to FE 999049 were significantly higher than for GONAL-F.
  • the applicants also investigated the impact of body weight. If relevant, the dose is converted into ⁇ g/kg for an average subject. This value of pg/kg and ⁇ 0.01 g kg are evaluated in a model with respect to distribution of oocytes retrieved as well as safety profile, and the optimal dose is identified.
  • the dose of FE999049 which fulfilled the first criterion was 12.1 g (mean 9.4 oocytes retrieved).
  • the distribution of oocytes is shown in Table 3 below.
  • a dose of 12.1 pg FE999049 provides retrieval of the most desirable number of oocytes in 60% of subjects in the low AMH group. This is a marked improvement on Gonal-F (most desirable number of oocytes in only 33% of subjects).
  • Table 4 shows the analysis of signs of excessive response in the low AMH strata (data are number of subjects). It can be seen that there were no indications of early OHSS of a moderate or severe nature and there were no incidences of preventative action being required; there are no concerns associated with the dose of 12.1 pg FE999049 in a patient having low AMH.
  • FIG 7 shows the effect of body weight on oocytes retrieved (for the low AMH strata), for the various doses.
  • the arrows indicate the number of oocytes retrieved from subjects of bodyweight between 45kg and 90kg treated at the 12.1 ⁇ g dose.
  • the variation between patients of bodyweight 45kg and those of 90kg is less than around 0.5 oocytes; in other words dosing by body weight is not required in patients with low AMH when dose of FE999049 is at least 12 ⁇ g, because there is not a significant variation in oocytes retrieved with body weight, at this dose.
  • a dose of, or dose equivalent to, 6 to 18 ⁇ g, for example 9 to 14 ⁇ g, for example 12 ⁇ g, human derived recombinant FSH is suitable for use in the treatment of infertility in a patient having serum AMH ⁇ 15 pmol/L, for example 0.05-14.9 pmol/L for example 5.0-14.9 pmol/L.
  • the dose provides an effective response while minimising risk of OHSS.
  • Fig 8 shows the effect of body weight on oocytes retrieved (for the high AMH strata), for the various doses.
  • the arrows indicate the number of oocytes retrieved from subjects of body weight between 45kg and 90kg treated at the 6.9 ⁇ g, 8.6 ⁇ g and 10.3 ⁇ g doses.
  • the variation is significant: for the 6.9 ⁇ g dose 6 additional oocytes will be retrieved from a 45 kg patient compared to a 90 kg patient; for the 8.6 ⁇ g dose 4 additional oocytes will be retrieved from a 45 kg patient compared to a 90 kg patient; and for the 10.1 ⁇ g dose 2.5 additional oocytes will be retrieved from a 45 kg patient compared to a 90 kg patient.
  • dosing by body weight has an impact in patients with high AMH when the dose of FE999049 is less than 12 ⁇ g, because there is a significant variation in oocytes retrieved with body weight, at these doses.
  • Table 5a shows a further breakdown of oocytes retrieved (from Table 2) by AMH. This shows the doses which fulfilled the first criterion (oocytes retrieved in the range 8-14) for each sub strata of AMH (boxes).
  • Table 5 b shows the analysis of patients where treatment was cancelled due to either excessive response or agonist triggering, for these subgroups. For example, one patient in the 25-34 pmol/L AMH strata cancelled due to excessive response following the dose of 10.3 pg and one patient in the 25-34 pmol/L AMH strata cancelled due to excessive response following the dose of 12.1 pg; one patient in the 35-45 pmol/L AMH strata cancelled following agonist triggering following dose of 10.3 pg; and one patient in the 35-45 pmol/L AMH strata cancelled following agonist triggering following dose of 6.9 pg.
  • AMH categories 3 AMH categories 2
  • a dose of, or dose equivalent to, 9 to 14 pg, for example 12 pg, human derived recombinant FSH is suitable for use in the treatment of infertility in a patient having serum AMH ⁇ 15 pmol/L, for example 0.05-14.9 pmol/L for example 5.0-14.9 pmol/L.
  • the dose provides an effective response while minimising risk of OHSS.
  • a dose of, or dose equivalent to, 5 to 12.5 pg, for example 6 to 10.5 pg, human derived recombinant FSH is suitable for use in the treatment of infertility in a patient having serum AMH ⁇ 5 pmol/L.
  • the dose provides an effective response while minimising risk of OHSS.
  • a (e.g. daily) dose of, or dose equivalent to, 0.09 to 0.19 pg human derived recombinant FSH per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of ⁇ 15 pmol/L.
  • a (e.g. daily) dose of, or dose equivalent to, 0.14 to 0.19 pg human derived recombinant FSH (preferably 0.15 to 0.16 pg human derived recombinant FSH) per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of 15 to 24.9 pmol/L.
  • a (e.g. daily) dose of, or dose equivalent to, 0.09 to 0.19 pg human derived recombinant FSH per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of 15 to 24.9 pmol/L.
  • a daily dose of, or dose equivalent to, 0.1 1 to 0.14 pg human derived recombinant FSH (preferably 0.12 to 0.13 pg human derived recombinant FSH) per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of 25 to 34.9 pmol/L.
  • a (e.g. daily) dose of, or dose equivalent to, 0.10 to 0.1 1 ⁇ g human derived recombinant FSH per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of ⁇ 35 pmol/L.
  • a (e.g. daily) dose of, or dose equivalent to, 0.15 to 0.21 ⁇ g (e.g. 0.16 ⁇ g) human derived recombinant FSH per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of ⁇ 15 pmol/L, for example for the first stimulation cycle with human derived recombinant FSH. .
  • a (e.g. daily) dose of, or dose equivalent to, 0.15 to 0.21 ⁇ g (e.g. 0.16 ⁇ g) human derived recombinant FSH per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of ⁇ 15 pmol/L, for example for the first stimulation cycle with human derived recombinant FSH.
  • body weight at this level of AMH.
  • the selected patients are about to undergo COS for in vitro fertilisation (IVF) / intracytoplasmic sperm injection (ICSI) by methods known in the art.
  • the pre-treatment protocol includes assessment/screening of the patient's serum AMH using the AMH Gen-ll enzyme linked immunosorbent assay kit (Beckman Coulter, Inc., Webster, Texas). This assay can detect AMH concentrations greater than 0.57 pmol/L with a minimum limit of quantitation of 1.1 pmol/L. AMH may be measured using other Assay kits (e.g. available from Roche).
  • the COS protocol proceeds in the usual manner apart from administration of the initial dose of FE 999049 according to AMH level at screening.
  • a patient with an AMH level of ⁇ 14.9 pmol/L would be administered an initial daily dose of approximately 12 ⁇ g FE 999049, a human derived recombinant FSH product manufactured according to the method of Example 6.
  • a patient with an AMH level of 15 to 24.9 pmol/L would receive an initial daily dose of 0.15 to 0.19 ⁇ g of the human derived recombinant FSH per kg bodyweight of the patient.
  • a patient with an AMH level of 25 to 34.9 pmol/L would receive an initial daily dose of 0.1 1 to 0.13 ⁇ g of the human derived recombinant FSH per kg bodyweight of the patient.
  • a patient with an AMH level of ⁇ 35 pmol/L would receive an initial daily dose of 0.10 to 0.1 1 ⁇ g of the human derived recombinant FSH per kg bodyweight of the patient.
  • Example 10A The doses in this protocol are less preferred that Example 10A.
  • the selected patients are about to undergo COS for in vitro fertilisation (IVF) / intracytoplasmic sperm injection (ICSI) by methods known in the art.
  • the pre-treatment protocol includes assessment/screening of the patient's serum AMH using the AMH Gen-ll enzyme linked immunosorbent assay kit (Beckman Coulter, Inc., Webster, Texas). This assay can detect AMH concentrations greater than 0.57 pmol/L with a minimum limit of quantitation of 1.1 pmol/L.
  • the COS protocol proceeds in the usual manner apart from administration of the initial dose of FE 999049 according to AMH level at screening in line with the following table.
  • a patient with an AMH level of 5-14.8 pmol/L would be administered 180 IU FSH in the form of approximately 8-1 1 ⁇ g FE 999049, a human derived recombinant FSH product manufactured according to the method of Example 6.
  • a patient with an AMH level of 30-44.9 pmol/L would be administered 120 I U FSH in the form of approximately 4-7 ⁇ g FE 999049, a human derived recombinant FSH product manufactured according to the method of Example 6.
  • the patient recombinant would be administered 120-180 IU FSH in the form of approximately 6-1 1 ⁇ g FE 999049, a human derived recombinant FSH product manufactured according to the method of Example 6.
  • FSH isoform composition of commercial gonadotrophin preparations a neglected aspect? Reprod Biomed Online. 9(2), 231-236.
  • Figs 1 , 2 and 3 Plasmid maps of the pFSHalpha/beta ,pST3 and pST6 expression vectors.
  • CMV Cytomegalovirus promoter
  • BGHp(A) Bovine Growth Hormone poly-adenylation sequence
  • fl ori fl origin of replication
  • SV40 Simian Virus 40 promoter
  • Neo Neomycin resistance marker
  • Hyg Hygromycin resistance marker
  • SV40 p(A) Simian Virus 40 poly-adenylation sequence
  • FSH A Follicle stimulating hormone alpha polypeptide
  • FSH B Follicle stimulating hormone beta polypeptide
  • ST3GAL4 a2,3- sialyltransferase
  • ST6GAL1 a2,6-sialyltransferase
  • ColEI ColEI origin of replication
  • Amp ampicillin resistance marker.
  • Beta-galactosamide alpha-2,6-sialyltransferase 1 Accession number NM 003032 Nucleotide sequence of ST6GAL1

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Abstract

Preparations including FSH, for example recombinant FSH, for use in the treatment of infertility.

Description

Composition for controlled ovarian stimulation
The present invention relates to compositions and pharmaceutical products for the treatment of infertility.
Assisted reproductive technology (ART) techniques such as in vitro fertilisation (IVF) are well known. These ART techniques generally require a step of controlled ovarian stimulation (COS), in which a cohort of follicles is stimulated to full maturity. Standard COS regimens include administration of gonadotrophins, such as follicle stimulating hormone (FSH) alone or in combination with luteinising hormone (LH) activity to stimulate follicular development, normally with administration of a GnRH analogue prior to and/or during stimulation to prevent premature LH surge. The pharmaceutical compositions generally used for COS include recombinant follicle stimulating hormone (rFSH), urinary derived FSH, recombinant FSH + LH preparations, urinary derived menotrophin [human menopausal gonadotrophin (hMG)] and highly purified human menopausal gonadotrophin (HP-hMG). IVF can be associated with a risk of ovarian hyperstimulation syndrome (OHSS), which can be life threatening in severe cases.
The ability to predict the response potential of women to controlled ovarian stimulation (COS) may allow the development of individualised COS protocols. This could, for example, reduce the risk of OHSS in women predicted to have an excessive response to stimulation, and/or improve pregnancy outcomes in women classed as poor responders. The serum concentration of anti-Mullerian hormone (AMH) is now established as a reliable marker of ovarian reserve. Decreasing levels of AMH are correlated with reduced ovarian response to gonadotrophins during COS. Further, high levels of AMH are a good predictor of excessive ovarian response, and an indicator of risk of OHSS.
In a preliminary study of women under 35 years old undergoing ART, the CONSORT dosing algorithm (incorporating basal FSH, BMI, age and AFC) was used to predict the optimal FSH starting dose for COS in women at risk of developing OHSS (Olivennes et. al., 2009). Individualising the dose did lead to adequate oocyte yield and good pregnancy rate. However, there were high rates of cancellations in the low dose group (75 IU FSH) due to inadequate response, and OHSS did occur in a significant proportion of the patients.
There is therefore a need for a composition for use in individualised COS protocols which provides adequate response to stimulation, and/or decreased risk of OHSS.
As indicated above, standard COS protocols may include administration of FSH. FSH is naturally secreted by the anterior pituitary gland and functions to support follicular development and ovulation. FSH comprises a 92 amino acid alpha sub-unit, also common to the other glycoprotein hormones LH and CG, and a 1 1 1 amino acid beta sub-unit unique to FSH that confers the biological specificity of the hormone (Pierce and Parsons, 1981 ). Each sub-unit is post translationally modified by the addition of complex carbohydrate residues. Both subunits carry 2 sites for N-linked glycan attachment, the alpha sub-unit at amino acids 52 and 78 and the beta sub-unit at amino acid residues 7 and 24 (Rathnam and Saxena, 1975, Saxena and Rathnam, 1976). FSH is thus glycosylated to about 30% by mass (Dias and Van Roey. 2001. Fox et al. 2001 ).
FSH purified from post-menopausal human urine has been used for many years in infertility treatment; both to promote ovulation in natural reproduction and to provide oocytes for assisted reproduction technologies. The currently approved recombinant FSH (rFSH) products for ovarian stimulation, such as follitropin alfa (GONAL-F, Merck Serono / EMD Serono) and follitropin beta (PUREGON / FOLLISTIM, MSD / Schering-Plough), are derived from a Chinese Hamster Ovary (CHO) cell line. Currently, no rFSH products from a human cell line are commercially available.
There is considerable heterogeneity associated with FSH preparations which relates to differences in the amounts of various isoforms present. Individual FSH isoforms exhibit identical amino acid sequences but differ in the extent to which they are post-translationally modified; particular isoforms are characterised by heterogeneity of the carbohydrate branch structures and differing amounts of sialic acid (a terminal sugar) incorporation, both of which appear to influence the specific isoform bioactivity.
Glycosylation of natural FSH is highly complex. The glycans in naturally derived pituitary FSH can contain a wide range of structures that can include combinations of mono-, bi- , tri- and tetra-antennary glycans (Pierce and Parsons, 1981. Ryan et al., 1987. Baenziger and Green, 1988). The glycans can carry further modifications: core fucosylation, bisecting glucosamine, chains extended with acetyl lactosamine, partial or complete sialylation, sialylation with a2,3 and a2,6 linkages, and sulphated galactosamine substituted for galactose (Dalpathado et al., 2006). Furthermore, there are differences between the distributions of glycan structures at the individual glycosylation sites. A comparable level of glycan complexity has been found in FSH derived from the serum of individuals and from the urine of postmenopausal women (Wide et al., 2007).
The glycosylation of recombinant FSH products reflects the range of glycosyl- transferases present in the host cell line. The commercially available rFSH products are derived from engineered Chinese hamster ovary cells (CHO cells). The range of glycan modifications in CHO cell derived rFSH are more limited than those found on the natural products. Examples of the reduced glycan heterogeneity found in CHO cell derived rFSH include a lack of bisecting glucosamine and a reduced content of core fucosylation and acetyl lactosamine extensions (Hard et al., 1990). In addition, CHO cells are only able to add sialic acid using the a2,3 linkage (Kagawa et al, 1988, Takeuchi et al, 1988, Svensson et al., 1990); CHO cell derived rFSH only includes a2,3-linked sialic acid and does not include a2,6-linked sialic acid.
Thus CHO cell derived FSH is different from naturally produced FSH (e.g. human Pituitary/ serum/ urinary FSH) which contains glycans with a mixture of a2,3 and a2,6-linked sialic acid, with a predominance of the former.
Further, it has also been demonstrated that the commercially available recombinant FSH preparation differs in the amounts of FSH with an isoelectric point (pi) of below 4 (considered the acidic isoforms) when compared to pituitary, serum or post-menopausal urine FSH (Ulloa-Aguirre et al. 1995). The amount of acidic isoforms in the urinary preparations was much higher as compared to the CHO cell derived recombinant products, Gonal-f (Merck Serono) and Puregon (Schering Plough) (Andersen et al. 2004). This must reflect a lower molar content of sialic acid in the recombinant FSH since the content of negatively-charged glycan modified with sulphate is low in recombinant FSH. The lower sialic acid content, compared to natural FSH, is a feature of both commercially available recombinant FSH products and may reflect a limitation in the manufacturing process.
The circulatory life-time of FSH has been documented for materials from a variety of sources. Some of these materials have been fractionated on the basis of overall molecular charge, as characterised by their pi, in which more acid equates to a higher negative charge. As previously stated the major contributor to overall molecular charge is the total sialic content of each FSH molecule. For instance, rFSH (Organon) has a sialic acid content of around 8 mol/mol, whereas urine-derived FSH has a higher sialic acid content (de Leeuw et al. 1996). The corresponding plasma clearance rates in the rat are 0.34 and 0.14 ml/min (Ulloa-Aguirre et al. 2003). In another example where a sample of recombinant FSH was split into high and low pi fractions, the in vivo potency of the high pi (lower sialic acid content) fraction was decreased and it had a shorter plasma half-life (D'Antonio et al. 1999). It has also been reported that the more basic FSH circulating during the later stages of the ovulation cycle is due to the down- regulation of a2,3 sialyl-transferase in the anterior pituitary which is caused by increasing levels of estradiol (Damian-Matsumara et al. 1999. Ulloa-Aguirre et al. 2001 ). Results for the a2,6 sialyl-transferase have not been reported.
Thus, as set out above, recombinant proteins expressed using the CHO system will differ from their natural counterparts in their type of terminal sialic acid linkages. This is an important consideration in the production of biologicals for pharmaceutical use since the carbohydrate moieties may contribute to the pharmacological attributes of the molecule.
The present applicants have developed a human derived recombinant FSH which is the subject of International Patent Application No. PCT/GB2009/000978, published as WO2009/127826A. Recombinant FSH with a mixture of both a2,3 and a2,6-linked sialic acid was made by engineering a human cell line to express both rFSH and a2,3 sialyltransferase. The expressed product is highly acidic and carries a mix of both a2,3- and a2,6-linked sialic acids; the latter provided by the endogenous sialyl transferase activity. It was found that the type of sialic acid linkage, a2,3- or a2,6-, can have a dramatic influence on biological clearance of FSH. Recombinant FSH with a mixture of both a2,3 and a2,6-linked sialic acid has two advantages over rFSH expressed in conventional CHO cells: first the material is more highly sialylated due to the combined activities of the two sialyltransferases; and secondly the material more closely resembles the natural FSH. This is likely to be more biologically appropriate compared to CHO cell derived recombinant products that have produce only a2,3 linked sialic acid (Kagawa et al, 1988, Takeuchi et al, 1988, Svensson et al., 1990) and have decreased sialic acid content (Ulloa-Aguirre et al. 1995., Andersen et al. 2004).
The rFSH product disclosed in International Patent Application No. PCT/GB2009/000978 contains branched glycan moieties. FSH comprises glycans (attached to the FSH glycoproteins) and these glycans may contain a wide variety of structures. As is well known in the art, branching (of a glycan) can occur with the result that the glycan may have 1 , 2, 3, 4 or more terminal sugar residues or "antennae"; glycans with 1 , 2, 3 or 4 terminal sugar residues or "antennae" are referred to respectively as mono-antennary, di-antennary, tri- antennary or tetra-antennary structures. Glycans may have sialylation presence on mono- antennary and/or di-antennary and/or tri-antennary and/or tetra-antennary structures. An example rFSH disclosed in International Patent Application No. PCT/GB2009/000978 included mono-sialylated, di- sialylated, tri- sialylated and tetra- sialylated glycan structures with relative amounts as follows: 9-15% mono-sialylated; 27 - 30% di— sialylated; 30 - 36% tri— sialylated and 25 - 29 % tetra-sialylated. As is well known, a mono-sialylated glycan structure carries one sialic acid residue; a di-sialylated glycan structure carries two sialic acid residues; a tri- sialylated glycan structure carries three sialic acid residues; and a tetra-sialylated glycan structure carries four sialic acid residues. Herein, terminology such as "X% mono-sialylated", "X% di-sialylated", "X% tri-sialylated" or "X% tetra-sialylated" refers to the number of glycan structures on FSH which are mono-, di, tri or tetra sialylated (respectively), expressed as a percentage (X%) of the total number of glycan structures on the FSH which are sialylated in any way (carry sialic acid). Thus, the phrase "30 - 36% tri-sialylated glycan structures" means that, of the total number of glycan structures on the FSH which carry sialic acid residues (that is, are sialylated), 30 to 36% of these glycan structures are tri sialylated (carry three sialic acid residues). The applicants have surprisingly found that FSH having a specific amount of tetra- sialylated glycan structures (which is different to that of the example rFSH product disclosed in PCT/GB2009/000978 mentioned above) is markedly more potent then recombinant FSH products which are currently on the market. The amino acid sequence of the applicant's products is the native sequence and is identical to natural human FSH and existing CHO-derived rFSH products. However, the present applicants have found that human derived recombinant FSH products (i.e. recombinant FSH produced or expressed in a human cell line e.g. made by engineering a human cell line) which have a mixture of both a2,3 and a2,6-linked sialic acid and/or a specific amount of tetra-sialylated glycan structures may be particularly effective when utilised in (e.g. individualised) COS protocols.
According to the present invention in a first aspect there is provided a product (e.g. a pharmaceutical composition) comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient (e.g. a patient having serum AMH level of 0.05 pmol/L or above, for example 0.5 pmol/L or above), wherein the product comprises a dose of, or a dose equivalent to, 1-24 μg, for example 2-24 μg, for example 2 to 15 μg, human derived recombinant FSH. Preferably the product comprises a dose of, or a dose equivalent to, 4.5 to 12.5 μg, for example 5 to 12.5 μg, for example 6 to 12.5 μg, for example 6.3 to 10.5 μg, human derived recombinant FSH.
According to the invention there is provided a product (e.g. a pharmaceutical composition) comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH level of <15 pmol/L (e.g. 0.05 pmol/L to 14.9 pmol/L), wherein the product comprises a (e.g. daily) dose of, or dose equivalent to, 9 to 14 μg, for example 1 1 to 13 μg, for example 12 μg human derived recombinant FSH. Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH. The dose provides an effective response while minimising risk of OHSS. Preferably the treatment of infertility comprising a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of <15 pmol/L (e.g. 0.05 pmol/L to 14.9 pmol/L).
According to the invention in a further aspect there is provided a product (e.g. a pharmaceutical composition) comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH level of ≥15 pmol/L, wherein the product comprises a (e.g. daily) dose of, or dose equivalent to, 5 to 12.5 μg, for example 6 to 10.5 μg human derived recombinant FSH. Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH. The dose provides an effective response while minimising risk of OHSS. Preferably the treatment of infertility comprising a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of ≥15 pmol/L. In one embodiment, the product is for use in the treatment of infertility in a patient having serum AMH level of 15 to 24.9 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 5 to 12 μg, for example 7 to 12 μg, for example 8.7 to 10 μg, human derived recombinant FSH (preferably 9 to 10 μg human derived recombinant FSH) In this embodiment, the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of 15 to 24.9 pmol/L. In another embodiment, the product is for use in the treatment of infertility in a patient having serum AMH level of 25 to 34.9 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 5 to 12 μg, for example 6 to 9 μg, for example 7 to 8 μghuman derived recombinant FSH (preferably 7.3 to 8 μg human derived recombinant FSH). In this embodiment, the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of 25 to 34.9 pmol/L. In another embodiment, the product is for use in the treatment of infertility in a patient having serum AMH level of≥ 35 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 5 to 1 1 μg, for example 6.3 to 7 μg, human derived recombinant FSH (preferably 6 to 7 μg human derived recombinant FSH). In this embodiment, the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of > 35 pmol/L.
The doses above may be for treatment of infertility in the patient's (subject's) first stimulation protocol. It will be appreciated that for further stimulation cycles, the doses may be adjusted according to actual ovarian response in the first cycle.
The applicants have found that it is generally necessary to retrieve in the region of nine oocytes in order to enable selection of two high quality oocytes for transfer.
The applicants have found that for subjects having low AMH (AMH < 15 pmol/L per litre) a reasonably high dose of recombinant FSH is required (for example 12 μg) to achieve this. At this dose, 8 to 14 oocytes will be retrieved from 60% of subjects with low AMH. This is an unexpected and significant improvement over treatment of subjects with low AMH treated with 150 IU Gonal-f, where 8 to 14 oocytes are retrieved from only 33% of subjects. The applicants have found that there is no need to adjust this dose according to the bodyweight of the patient.
However, 60 % of the population (and 80% of women under 30 treated for infertility) have high AMH (that is, AMH of ≥15 pmol/L). For these subjects it is generally fairly straightforward to retrieve a mean of 9 to 1 1 oocytes; the problem with stimulation protocols is the risk of OHSS. The applicants have found that in patients dosed at low doses of human recombinant FSH that there is a relationship between oocytes retrieved and body weight of the subject. This means that there may be a risk associated with treatment with a fixed dose of FSH (which is usual in the art). The present applicants have established a relationship between dose of FSH and AMH level and weight of the subject which provides an improved safety profile (reduced risk of OHSS) with acceptable or improved oocyte retrieval compared to the known treatment protocols (see example 10).
According to the invention in a further aspect there is provided a product (e.g. a pharmaceutical composition) comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH level of≥15 pmol/L, wherein the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.09 to 0.19 μg (for example 0.09 to 0.17 μg) human derived recombinant FSH per kg bodyweight of the patient. Preferably the treatment of infertility comprises a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of ≥15 pmol/L. In one embodiment, the product is for use in the treatment of infertility in a patient having serum AMH level of 15 to 24.9 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.14 to 0.19 μg human derived recombinant FSH (preferably 0.15 to 0.16 μg human derived recombinant FSH) per kg bodyweight of the patient. In this embodiment, the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of 15 to 24.9 pmol/L. In another embodiment, the product is for use in the treatment of infertility in a patient having serum AMH level of 25 to 34.9 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.1 1 to 0.14 μg human derived recombinant FSH (preferably 0.12 to 0.13 μg human derived recombinant FSH) per kg bodyweight of the patient. In this embodiment, the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of 25 to 34.9 pmol/L. In a still further embodiment, the product is for use in the treatment of infertility in a patient having serum AMH level of ≥ 35 pmol/L, and the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.10 to 0.1 1 μg human derived recombinant FSH per kg bodyweight of the patient. In this embodiment, the treatment of infertility may comprise a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of > 35 pmol/L. Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH. The doses provide an effective response while minimising risk of OHSS.
The doses above may be for treatment of infertility in the patient's (subject's) first stimulation protocol. It will be appreciated that for further stimulation cycles, the doses may be adjusted according to actual ovarian response in the first cycle.
According to the invention in a still further aspect there is provided a product (e.g. a pharmaceutical composition) comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH level of <15 pmol/L, wherein the product is for administration at a (e.g. daily) dose of, or dose equivalent to, 0.15 to 0.21 μg, (for example 0.19 to 0.21 μg) human derived recombinant FSH per kg bodyweight of the patient. Preferably the treatment of infertility comprises a step of determining (e.g. measuring) the serum AMH level of the patient, and administering the dose to a patient having serum AMH level of <15 pmol/L. However, it is not required that patients having serum AMH level of <15 pmol/L are dosed by body weight. It will be appreciated that these doses may be readily converted to treat patients with dosing according to their BMI, using conversions well known in the art.
The product (e.g. pharmaceutical compostion) may be for use in the treatment of infertility in a patient having serum AMH of 5.0-14.9 pmol/L, wherein the product comprises a dose of, or dose equivalent to, 6 to 18 μg, for example 8 to 1 1 μg, for example 8.5 to 10.2 μg human derived recombinant FSH. The product may be for use in the treatment of infertility in a patient having serum AMH 15.0-29.9 pmol/L, wherein the product comprises a dose of, or a dose equivalent to, 4.8 to 15 μg, for example 6 to 9 μg, for example 6.8 to 8.5 μg human derived recombinant FSH. The product may be for use in the treatment of infertility in a patient having serum AMH 30-44.9 pmol/L, wherein the product comprises a dose of, or a dose equivalent to, 3.6 to 12 μg, for example 4 to 7 μg, for example 5.1 to 6.8 μg human derived recombinant FSH. The product may be for use in the treatment of infertility in a patient having serum AMH 45 pmol/L or greater, wherein the product comprises a dose of, or a dose equivalent to, 2 to 9 μg, for example 2.4 to 9 μg (for example 3.4 to 5.1 μg) or 2 to 5 μg human derived recombinant FSH. The product may comprise follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH of 5 pmol/L or less, wherein the product comprises a dose of, or a dose equivalent to 7.2 to 24 μg, for example 10 to 15 μg for example 10.2 to 13.6 μg, human derived recombinant FSH. The product may be for use in the treatment of infertility in a patient wherein the product comprises a dose of, or dose equivalent to, 4.8 to 18 μg, for example 6 to 1 1 μg, for example 6.8 to 10.2 μg human derived recombinant FSH. Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH.
Preferably the rFSH (e.g. human cell line derived recombinant FSH) includes a2,3- and a2,6- sialylation. The FSH (rFSH) for use according to the invention may have 1 % to 99% of the total sialylation being a2,3-sialylation. The FSH (rFSH) according to the invention may have 1 % to 99% of the total sialylation being a2,6-sialylation. Preferably, 50 to 70%, for example 60 to 69%, for example about 65%, of the total sialylation is a2,3-sialylation. Preferably 25 to 50%, for example 30 to 50 %, for example 31 to 38%, for example about 35%, of the total sialylation is a2,6- sialylation.
Preferably the rFSH (e.g. human cell line derived recombinant FSH) includes mono-, di- , tri- and tetra-sialylated glycan structures, wherein 15-24%, for example 17-23% of the sialylated glycan structures are tetrasialylated glycan structures (e.g. as shown by WAX analysis of charged glycans, as set out in the Examples below). The FSH comprises glycans (attached to the FSH glycoproteins). It is well known that glycans in FSH may contain a wide variety of structures. These may include combinations of mono, bi, tri and tetra-antennary glycans. Herein, terminology such as "X% of the sialylated glycan structures are tetrasialylated glycan structures" refers to the number of glycan structures on the FSH which are tetra sialylated, i.e. carry four sialic acid residues, expressed as a percentage (X%) of the total number of glycan structures on the FSH which are sialylated in any way (carry sialic acid). Thus, the phrase "15-24% of the sialylated glycan structures are tetrasialylated glycan structures" means that, of the total number of glycan structures on FSH which carry sialic acid residues (that is, are sialylated), 15 to 24% of these glycan structures are tetra sialylated (carry four sialic acid residues).
The rFSH may be present as a single isoform or as a mixture of isoforms.
The applicants have devised "individualised" COS protocols wherein specific doses of recombinant FSH having specific characteristics are used to treat patients based on their specific AMH levels, thereby increasing the likelihood of adequate response to stimulation (e.g. in patients having a low response potential), and/or decreased risk of OHSS (e.g. in patients classed as high or excessive responders).
The serum level of AMH may be determined (e.g. measured) by any method known in the art. Preferably the serum AMH level is measured using the AMH Gen-ll enzyme linked immunosorbent assay, a kit (Beckman Coulter, Inc., Webster, Texas). This assay can detect can detect AMH concentrations greater than 0.57 pmol/L with a minimum limit of quantitation of 1.1 pmol/L. Other assays may be used.
Herein, serum AMH values are generally recited in terms of pmol/L. This may be converted to ng/mL using the conversion equation 1 ng/ml AMH = 7.1 pmol/L AMH.
Herein the terms "patient" and "subject" are used interchangeably.
The product (e.g. pharmaceutical composition) preferably comprises a daily dose of, or a daily dose equivalent to, the amounts of human derived rFSH defined above, herein, and in the claims. The (daily) dose may be an initial dose (i.e. it may be reduced, increased, or maintained during the treatment).
The product (e.g. pharmaceutical composition) may be for (daily) administration of FSH starting on day one of treatment and continuing for seven to thirteen days, for example nine to thirteen days, for example 10 to 13 days, for example 10 to 1 1 days. The product (e.g. pharmaceutical composition) may be for administration 12 to 16, e.g. 13 to 15, e.g. 14 days after administration of (e.g. after initiation of administration of, e.g. after initiation of daily administration of) a GnRH agonist (e.g. Synarel, Lupron, Decapeptyl). The product (e.g. pharmaceutical composition) may be for administration with a GnRH agonist. The product (e.g. pharmaceutical composition) may be for administration prior to administration of a GnRH antagonist (e.g. ganirelix, cetrorelix), for example for administration five or six days prior to administration of a GnRH antagonist. The product (e.g. pharmaceutical composition) may be for administration with a GnRH antagonist. Preferably the product (e.g. pharmaceutical composition) is for administration prior to administration of a high (ovulatory) dose of hCG (for example 4,000 to 1 1 ,000 IU hCG, e.g. 5,000 IU hCG, 10,000 IU hCG etc.; or 150 to 350 microgram recombinant hCG, for example 250 microgram recombinant hCG) to induce final follicular maturation.
It will be appreciated that the product may be for dosing at frequencies more (or less) than daily, in which case the relevant doses will be equivalent to the (daily) doses specified herein.
Herein the term "treatment of infertility" includes treatment of infertility by controlled ovarian stimulation (COS) or methods which include a step or stage of controlled ovarian stimulation (COS), for example Intra Uterine Insemination (IUI), in vitro fertilisation (IVF), or intracytoplasmic sperm injection (ICSI). The term "treatment of infertility" includes treatment of infertility by ovulation induction (Ol) or by methods which include a step or stage of ovulation induction (01). The term "treatment of infertility" includes treatment of infertility in a subject having tubal or unexplained infertility, including treatment of infertility in a subject having endometriosis, for example stage I or stage II endometriosis, and/or in a subject having anovulatory infertility, for example WHO type II anovulatory infertility, and/or in a subject with a partner with male factor infertility. The product (or composition) may be for (use in) the treatment of infertility (and/or for controlled ovarian stimulation) in a subject having endometriosis, for example in a subject having stage I or stage II endometriosis, as defined by The American Society for Reproductive Medicine (ASRM) classification system for the various stages of endometriosis, (stage IV most severe; stage I least severe) [American Society for Reproductive Medicine. Revised American Society for Reproductive Medicine classification of endometriosis: 1996. Fertil Steril 1997; 67,817 821 .].
The product (composition) may be for (use in) the treatment of infertility (and/or for controlled ovarian stimulation) in a subject having normal serum FSH level of 1 to 16 IU/L, for example 1 to 12 IU/L, in the early follicular phase.
The product (composition) may be for (use in) the treatment of infertility (and/or for controlled ovarian stimulation) in a subject aged 18 to 42 years, for example 25 to 37 years. The product may be for (use in) the treatment of infertility (and/or for controlled ovarian stimulation) in a subject having BMI >1 and BMI < 35 kg/m2, for example a subject having BMI >18 and BMI < 25 kg/m2, for example a subject having BMI >20 and BMI < 25 kg/m2.
The rFSH may preferably include 27 - 33%, for example 30 - 32%, tri-sialylated glycan structures. The rFSH may preferably include 24 - 33%, for example 26 - 30%, di-sialylated glycan structures. The rFSH may preferably include 12 - 21 %, for example 15 - 17%, mono- sialylated glycan structures. The rFSH preferably includes mono-sialylated, di- sialylated, tri- sialylated and tetra- sialylated glycan structures with relative amounts as follows: 15 to 17% mono-sialylated; 26 - 30% di-sialylated; 27 - 33% (e.g. 29 to 32%, e.g 30-32%, e.g 30 to 31 %) tri-sialylated and 17 - 23 % tetra-sialylated (e.g. as shown by WAX analysis of charged glycans, as set out in the Examples). The rFSH may include from 0 to 7%, for example 0.1 to 7%, for example 3 to 6%, for example 5 to 6%, neutral sialylated structures. The FSH comprises glycans (attached to the FSH glycoproteins). Herein, terminology such as "X% mono-sialylated", "X% di-sialylated", "X% tri-sialylated" or "X% tetra-sialylated" refers to the number of glycan structures on FSH which are mono-, di, tri or tetra sialylated (respectively), expressed as a percentage (X%) of the total number of glycan structures on the FSH which are sialylated in any way (carry sialic acid). Thus, the phrase "27 - 33% tri-sialylated glycan structures" means that, of the total number of glycan structures on FSH which carry sialic acid residues (that is, are sialylated), 27 to 33% of these glycan structures are tri sialylated (carry three sialic acid residues).
The rFSH may have a sialic acid content [expressed in terms of a ratio of moles of sialic acid to moles of protein] of 6 mol/mol or greater, for example between 6 mol/mol and 15 mol/mol, e.g between 8 mol/mol and 14 mol/mol, for example between 10 mol/mol and 14 mol/mol, e.g between 1 1 mol/mol and 14 mol/mol, e.g between 12 mol/mol and 14 mol/mol, e.g. between 12 mol/mol and 13 mol/mol. The rFSH may be produced or expressed in a human cell line.
The FSH (rFSH) for use according to the invention may have 1 % to 99% of the total sialylation being a2,3-sialylation. The rFSH may have 10% or more of the total sialylation being a2,3-sialylation. For example, 20, 30, 40, 50, 60, 70, 80 or 90% or more of the total sialylation may be a2,3-sialylation. The rFSH may preferably include a2,3-sialylation in an amount which is from 50 to 70% of the total sialylation, for example from 60 to 69% of the total sialylation, for example from 63 to 67%, for example around 65% of the total sialylation. The FSH (rFSH) for use according to the invention may have 1 % to 99% of the total sialylation being a2,6- sialylation. The rFSH (or rFSH preparation) of the invention may have 5% or more, for example 5% to 99%, of the total sialylation being a2,6-sialylation. The rFSH may have 50% or less of the total sialylation being a2,6-sialylation. The rFSH may preferably include a2,6- sialylation in an amount which is from 25 to 50% of the total sialylation, for example from 30 to 50% of the total sialylation, for example from 31 to 38%, for example around 35% of the total sialylation. By sialylation it is meant the amount of sialic residues present on the FSH carbohydrate structures. a2,3-sialylation means sialylation at the 2,3 position (as is well known in the art) and a2,6 sialylation at the 2,6 position (also well known in the art). Thus "% of the total sialylation may be a 2,3 sialylation" refers to the % of the total number of sialic acid residues present in the FSH which are sialylated in the 2,3 position. The term "% of the total sialylation being a2,6-sialylation" refers to the % of the total number of sialic acid residues present in the FSH which are sialylated in the 2,6 position.
The rFSH may have a sialic acid content (amount of sialylation per FSH molecule) of (based on the mass of protein, rather than the mass of protein plus carbohydrate) of 6% or greater (e.g. between 6% and 15%, e.g. between 7% and 13%, e.g. between 8% and 12%, e.g. between 1 1 % and 15%, e.g. between 12% and 14%) by mass.
The rFSH may be rFSH or a rFSH preparation in which 16 % or fewer (e.g. 0.1 to 16%) of the glycans comprise (e.g. carry) bisecting N-acetylglucosamine (bisecting GlcNAc or bisGlcNAc). Preferably the rFSH (or rFSH preparation) is an rFSH or rFSH preparation in which 8 to 14.5% of the glycans comprise (e.g. carry) a bisecting N-acetylglucosamine (bisecting GlcNAc or bisGlcNAc).
It will be understood that FSH comprises glycans attached to the FSH glycoproteins. It will also be understood that 100% of the glycans refers to or means all of the glycans attached to the FSH glycoproteins. Thus, herein, the terminology "8 to 14.5% of the glycans comprise (carry) bisecting N-acetylglucosamine" means that 8 to 14.5% of the total number of glycans attached to the FSH glycoproteins include/carry bisecting N-acetylglucosamine; "16% or fewer of the glycans comprise (carry) bisecting N-acetylglucosamine" means that 16 % or fewer of the total number of glycans attached to the FSH glycoproteins include/carry bisecting N- acetylglucosamine, and so on.
The applicants have found that recombinant FSH (rFSH preparations; rFSH compositions) in which 16% or fewer (e.g. 8 to 14.5%) of the glycans comprised in the FSH glycoproteins carry bisecting GlcNac may have advantageous pharmacokinetic properties. It is believed the advantageous properties may arise because the amount of glycans which carry bisecting GlcNac is similar to that in the human urinary derived product Bravelle, which is rather less than that of other recombinant FSH preparations such as those disclosed in WO2012/017058.
The rFSH (or rFSH preparation) may be an rFSH or rFSH preparation in which 20% or more of the glycans comprise (e.g. carry) N-Acetylgalactosamine (GalNAc), for example in which 20% or more of the glycans comprise (e.g. carry) a terminal GalNAc. Preferably the rFSH (or rFSH preparation) is an FSH or FSH preparation in which the 40 to 55%, for example 42% to 52%, of the glycans comprise (e.g. carry) GalNAc. Preferably the rFSH (or rFSH preparation) is an FSH or FSH preparation in which the 40 to 55%, for example 42% to 52%, of the glycans comprise (e.g. carry) terminal GalNAc.
It will be understood that FSH comprises glycans attached to the FSH glycoproteins. It will also be understood that 100% of the glycans refers to or means all of the glycans attached to the FSH glycoproteins. Thus, herein, the terminology "wherein 20% or more of the glycans comprise (e.g. carry) GalNAc" means that 20% or more of the total number of glycans attached to the FSH glycoproteins include/carry N-Acetylgalactosamine (GalNAc); "40 to 55%, for example 42% to 52%, of the glycans comprise (e.g. carry) terminal GalNAc" means that 40 to 55 %, for example 42% to 52%, of the total number of glycans attached to the FSH glycoproteins include/carry terminal GalNAc, and so on.
It appears that the availability of the a2,6- linkage increases the number of tetra sialylated structures, compared to CHO cell derived products which have only the a2,3- linkage available. The applicants have also found that their rFSH is distinguished over other approved products because of the sugar composition: it includes, or may include, a specific amount of GalNac. This may be linked to tetrasialylation and potency because the 2,6- sialylation is associated with GalNac. In other words, the present applicants have developed an rFSH product which includes specific characteristics (2,6- linker sites, GalNac) which provide rFSH with high degree of sialylation, which appears to lead to improved potency in vivo.
The rFSH (or rFSH preparation) may have 16 to 24% of the glycans comprising (e.g. terminal) 1 fucose-lewis, for example 16.5 to 18% of the glycans comprising (e.g. terminal) 1 fucose-lewis. The rFSH (or rFSH preparation) may have 1 .5 to 4.5%, for example 2 to 4%, for example 3.7%, of the glycans comprising (e.g. terminal) 2 fucose -lewis. The content of fucose-lewis may have an effect on potency.
The rFSH may be produced or expressed in a human cell line, for example a Per.C6 cell line, a HEK293 cell line, a HT1080 cell line etc.. This may simplify (and render more efficient) the production method because manipulation and control of e.g. the cell growth medium to retain sialylation may be less critical than with known processes. The method may also be more efficient because there is little basic rFSH produced compared to production of known rFSH products; more acidic rFSH is produced and separation/removal of basic FSH is less problematic. The rFSH may be produced or expressed in a PER.C6® cell line, a PER.C6® derived cell line or a modified PER.C6® cell line. rFSH which is produced or expressed in a human cell line (e.g. PER.C6® cell line, HEK293 cell line, HT1080 cell line etc.) will include some a2,6-linked sialic acids (a2,6 sialylation) provided by endogenous sialyl transferase activity [of the cell line] and will include some a2,3-linked sialic acids (a2,3 sialylation) provided by endogenous sialyl transferase activity. The cell line may be modified using a2,3- sialyltransferase. The cell line may be modified using a2,6-sialyltransferase. Alternatively or additionally, the rFSH may include a2,6-linked sialic acids (a2,6 sialylation) provided by endogenous sialyl transferase activity [of the cell line]. Herein, the term "human derived recombinant FSH" means recombinant FSH which is produced or expressed in a human cell line (e.g. recombinant FSH made by engineering a human cell line).
The rFSH may be produced using a2,3- and/or a2,6-sialyltransferase. In an example, rFSH is produced using a2,3- sialyltransferase. The rFSH may include a2,6-linked sialic acids (a2,6 sialylation) provided by endogenous sialyl transferase activity.
The product may be a pharmaceutical composition. The pharmaceutical composition is for the treatment of infertility. The treatment of infertility may comprise assisted reproductive technologies (ART), ovulation induction or intrauterine insemination (IUI). The pharmaceutical composition may be used, for example, in medical indications where known FSH preparations are used.
The product or composition can be formulated into well-known compositions for any route of drug administration, e.g. oral, rectal, parenteral, transdermal (e.g. patch technology), intravenous, intramuscular, subcutaneous, intrasusternal, intravaginal, intraperitoneal, local (powders, ointments or drops) or as a buccal or nasal spray. A typical composition comprises a pharmaceutically acceptable carrier, such as aqueous solution, non toxic excipients, including salts and preservatives, buffers and the like, as described in Remington's Pharmaceutical Sciences fifteenth edition (Matt Publishing Company, 1975), at pages 1405 to 1412 and 1461 - 87, and the national formulary XIV fourteenth edition (American Pharmaceutical Association, 1975), among others.
Examples of suitable aqueous and non-aqueous pharmaceutical carriers, diluents, solvents or vehicles include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), carboxymethylcellulose and suitable mixtures thereof, vegetable oils (such as olive oil), and injectible organic esters such as ethyl oleate. The compositions of the present invention also can contain additives such as but not limited to preservatives, wetting agents, emulsifying agents, surfactants and dispersing agents. Antibacterial and antifungal agents can be included to prevent growth of microbes and includes, for example, m-cresol, benzyl alcohol, paraben, chlorobutanol, phenol, sorbic acid, and the like. If a preservative is included, benzyl alcohol, phenol and/or m-cresol are preferred; however, the preservative is by no means limited to these examples. Furthermore, it may be desirable to include isotonic agents such as sugars, sodium chloride, and the like. The product or composition may further comprise a salt comprising a pharmaceutically acceptable alkali metal cation selected from the group consisting of Na+- or K+- salts, or a combination thereof. Preferably the salt is a Na+- salt, for example NaCI or Na2S04.
Preferably the product or composition comprises recombinant FSH and one or more of
Polysorbate 20, L-methionine, phenol, disodium sulphate and sodium phosphate buffer.
In some cases, to effect prolonged action it is desirable to slow the absorption of FSH (and other active ingredients, if present) from subcutaneous or intramuscular injection. This can be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of FSH then depends upon its rate of dissolution which, in turn, can depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered FSH combination form is accomplished by dissolving or suspending the FSH combination in an oil vehicle. Injectable depot forms can be made by forming microencapsule matrices of the FSH (and other agents, if present) in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of FSH to polymer and the nature of the particular polymer employed, the rate of FSH release can be controlled. Examples of other biodegradable polymers include polyvinylpyrrolidone, poly(orthoesters), poly(anhydrides) etc. Depot injectable formulations are also prepared by entrapping the FSH in liposomes or microemulsions which are compatible with body tissues.
Injectable formulations can be sterilized, for example, by filtration through a bacterial- retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium just prior to use. Injectable formulations can be supplied in any suitable container, e.g. vial, pre-filled syringe, injection cartridges, and the like.
The product or composition may be formulated for single use or for multiple use
(multiple dose). If the product or composition is formulated for multiple use, it is preferred that a preservative is included. If a preservative is included, benzyl alcohol, phenol and/or m-cresol are preferred; however, the preservative is by no means limited to these examples. The single use or multiple use formulated product or composition may further comprise a salt comprising a pharmaceutically acceptable alkali metal cation selected from the group consisting of Na+- or K+- salts, or a combination thereof. Preferably the salt is a Na+- salt, for example NaCI or Na2S04. The product or composition may be included in a container such as a vial, prefilled cartridge (e.g. for single administration or multiple use) or an injection device such as a "pen" for e.g. administration of multiple doses .
The product or composition may be a formulation (e.g. injectable formulation) including FSH (optionally with hCG, LH, LH activity etc.) The LH activity, if present, may originate from LH or human chorionic gonadotropin, hCG. If there is more than one active ingredient (i.e. FSH and e.g. hCG or LH) these may be suitable for administration separately or together. If administered separately, administration can be sequential. The product can be supplied in any appropriate package. For example, a product can include a number of containers (e.g. pre- filled syringes or vials) containing either FSH or hCG, or a combination (or combination) of both FSH and hCG. The hCG may be recombinant hCG or urinary hCG. If the product includes a number of containers (e.g. pre-filled syringes or vials) containing FSH, e.g. recombinant FSH, each container may include the same amount of FSH. One or more containers may include different amounts of FSH. The syringes or vials may be packaged in a blister package or other means to maintain sterility. Any product can optionally contain instructions for using the FSH (and e.g. hCG if present) formulations. The pH and exact concentration of the various components of the pharmaceutical composition are adjusted in accordance with routine practice in this field. See GOODMAN and GILMAN's THE PHARMACOLOGICAL BASIS FOR THERAPEUTICES, 7th ed. In a preferred embodiment, the compositions of the invention are supplied as compositions for parenteral administration. General methods for the preparation of the parenteral formulations are known in the art and are described in REMINGTON; THE SCIENCE AND PRACTICE OF PHARMACY, supra, at pages 780-820. The parenteral compositions can be supplied in liquid formulation or as a solid which will be mixed with a sterile injectable medium just prior to administration. In an especially preferred embodiment, the parenteral compositions are supplied in dosage unit form for ease of administration and uniformity of dosage.
According to the present invention in a further aspect there is provided a method of treatment of infertility comprising: (a) measuring the serum AMH level of a subject; and (b) administration to the subject a dose of, or a dose equivalent to, 1-24 μg, for example 2-24 μg, for example 2 to 15 μg, human derived recombinant FSH. Preferably the dose is, or is equivalent to, 4.5 to 12.5 μg, for example 5 to 12.5 μg, for example 6 to 12.5 μg, for example 6.3 to 12 μg, human derived recombinant FSH.
According to the present invention in a further aspect there is provided a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject; and (b) administering a (e.g. daily) dose of, or dose equivalent to, 9 to 14 μg, for example 1 1 to 13 μg, for example 12 μg human derived recombinant follicle stimulating hormone (FSH) to a (the) subject having serum AMH level of <15 pmol/L (e.g. 0.05 pmol/L to 14.9 pmol/L). Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH. The dose provides an effective response while minimising risk of OHSS.
According to the present invention in a further aspect there is provided a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject; and (b) administering a (e.g. daily) dose of, or dose equivalent to, 5 to 12.5 μg human derived recombinant follicle stimulating hormone (FSH) to a (the) subject having serum AMH level of ≥15 pmol/L. The (e.g. daily) dose may be, or be equivalent to, 6 to 10 μg human derived recombinant follicle stimulating hormone (FSH). Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH. The dose provides an effective response while minimising risk of OHSS.
In one embodiment, the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 5 to 12 μg, for example 7 to 12 μg, for example 8.7 to 10 μg, human derived recombinant FSH (preferably 9 to 10 μg human derived recombinant FSH) to a (the) subject having serum AMH level of 15 to 24.9 pmol/L In another embodiment, the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 5 to 12 μg human derived recombinant FSH (for example 7 to 12 μg, for example 6 to 9 μg, for example 7 to 8 μg, for example 7.3 to 8 μg human derived recombinant FSH) to a (the) subject having serum AMH level of 25 to 34.9 pmol/L. In another embodiment, the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 5 to 1 1 μg human derived recombinant FSH (for example 6 to 7 μg, for example 6.3 to 7 μg, human derived recombinant FSH) to a (the) subject having serum AMH level of≥ 35 pmol/L.
According to the present invention in a further aspect there is provided a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject; and (b) administering a (e.g. daily) dose of, or dose equivalent to, 0.09 to 0.19 μg (for example 0.09 to 0.17 μg) human derived recombinant FSH per kg bodyweight of the subject, wherein the subject has serum AMH level of≥15 pmol/L. Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH. The dose provides an effective response while minimising risk of OHSS.
In one embodiment, the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 0.14 to 0.19 μg human derived recombinant FSH (preferably 0.15 to 0.16 μg human derived recombinant FSH) per kg bodyweight of the subject, the subject having serum AMH level of 15 to 24.9 pmol/L . In another embodiment, the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 0.1 1 to 0.14 μg human derived recombinant FSH (preferably 0.12 to 0.13 μg human derived recombinant FSH) per kg bodyweight of the subject, the subject having serum AMH level of 25 to 34.9 pmol/L. In another embodiment, the method includes a step of administering a (e.g. daily) dose of, or dose equivalent to, 0.10 to 0.1 1 μg human derived recombinant FSH per kg bodyweight of the subject, the subject having serum AMH level of ≥35 pmol/L. Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH. These doses provide an effective response while minimising risk of OHSS.
According to the present invention in a further aspect there is provided a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject; and (b) administering a (e.g. daily) dose of, or dose equivalent to, 0.15 to 0.21 μg (for example 0.19 to 0.21 μg) human derived recombinant FSH per kg bodyweight of the subject, wherein the subject has serum AMH level of <15 pmol/L.
The administration preferably comprises a daily dose of, or a daily dose equivalent to, the amount of FSH defined above and in the claims. The (daily) dose may be an initial dose (it may be reduced, increased, or maintained during the treatment).
The method may be a method of treatment of infertility in the patient's (subject's) first stimulation protocol. It will be appreciated that for further stimulation cycles, the doses may be adjusted according to actual ovarian response in the first cycle.
According to the present invention in a further aspect there is provided a method of treatment of infertility comprising: (a) determining (e.g. measuring) the serum AMH level of a subject;
and (b) if the subject has serum AMH level of <15 pmol/L (e.g. 0.05 pmol/L to 14.9 pmol/L), administering to the subject a dose of, or dose equivalent to, 10 to 14 μg, for example 1 1 to 13 μg, for example 12 μg, human derived recombinant follicle stimulating hormone (FSH); or
if the subject has serum AMH level of 15 to 24.9 pmol/L, administering to the subject a dose of, or dose equivalent to, 0.14 to 0.19 μg human derived recombinant FSH (preferably 0.15 to 0.16 μg human derived recombinant FSH) per kg bodyweight of the subject; or
if the subject has serum AMH level of 25 to 34.9 pmol/L pmol/L, administering to the subject a dose of, or dose equivalent to, 0.1 1 to 0.14 μg human derived recombinant FSH (preferably 0.12 to 0.13 μg human derived recombinant FSH) per kg bodyweight of the subject; or
if the subject has serum AMH level of≥35 pmol/L pmol/L, administering to the subject a dose of, or dose equivalent to, 0.10 to 0.1 1 μg human derived recombinant FSH per kg bodyweight of the subject.
For a patient (subject) having serum AMH of 5.0-14.9 pmol/L, a dose of, or dose equivalent to, 6 to 18 μg, for example 8 to 1 1 μg, for example 8.5 to 10.2 μg human derived recombinant FSH may be administered. For a patient (subject) having serum AMH 15.0-29.9 pmol/L, a dose of, or a dose equivalent to, 4.8 to 15 μg, for example 6 to 9 μg, for example 6.8 to 8.5 μg human derived recombinant FSH may be administered. For a patient (subject) having serum AMH 30-44.9 pmol/L, a dose of, or a dose equivalent to, 3.6 to 12 μg, for example 4 to 7 μg, for example 5.1 to 6.8 μg human derived recombinant FSH may be administered. For a patient (subject) having serum AMH 45 pmol/L or greater, a dose of, or a dose equivalent to, 2 to 9 μg, for example 2.4 to 9 μg (for example 3.4 to 5.1 μg) or 2 to 5 μg human derived recombinant FSH may be administered. For a patient (subject) having serum AMH of 5 pmol/L or less, a dose of, or a dose equivalent to 7.2 to 24 μg, for example 10 to 15 μg for example 10.2 to 13.6 μg, human derived recombinant FSH may be administered. In some examples, a dose of, or dose equivalent to, 4.8 to 18 μg, for example 6 to 1 1 μg, for example 6.8 to 10.2 μg human derived recombinant FSH is administered. Preferably the FSH is a recombinant FSH ("rFSH" or "recFSH"). Preferably the FSH is a human cell line derived recombinant FSH. The administration preferably comprises a daily dose of, or a daily dose equivalent to, the amount of FSH defined above and in the claims. The (daily) dose may be an initial dose (it may be reduced, increased, or maintained during the treatment.
Detailed description of the invention
The present invention will now be described in more detail with reference to the attached drawings in which:
Figure 1 shows a plasmid map of the pFSHalpha/beta expression vector;
Figure 2 shows the a2,3-sialyltransferase (ST3GAL4) expression vector;
Figure 3 shows the a2,6-sialyltransferase (ST6GAL1 ) expression vector;
Figure 4 shows % abundance sialic acid distribution of examples of recombinant FSH produced by PER.C6® cells stably expressing FSH after engineering with a2,3- sialyltransferase;
Figure 5 shows % abundance of glycan charge distribution of examples of recombinant FSH produced by PER.C6® cells stably expressing FSH after engineering with a2,3- sialyltransferase;
Figure 6 shows a comparison of concentration of inhibin-B following administration of 225IU Gonal f (bottom line, dotted line) and 225 IU of the Example (top line, full line) of Invention; Fig 7 shows the effect of body weight on oocytes retrieved in the low AMH treatment group (Example 10, 10A); and
Fig 8 shows the effect of Body weight on oocytes retrieved in the high AMH treatment group
Sequence Selection
Human FSH
The coding region of the gene for the FSH alpha polypeptide was used to according to Fiddes and Goodman. (1981 ). The sequence is banked as AH007338 and at the time of construction there were no other variants of this protein sequence. The sequence is referred herein as SEQ ID NO:1.
The coding region of the gene for FSH beta polypeptide was used according to Keene et al (1989). The sequence is banked as NM_000510 and at the time of construction there were no other variants of this protein sequence. The sequence is referred herein as SEQ ID NO: 2
Sialyltransferase
a2,3-Sialyltransferase - The coding region of the gene for beta-galactoside alpha-2,3- sialyltransferase 4 (a2,3-sialyltransferase, ST3GAL4) was used according to Kitagawa and Paulson (1994). The sequence is banked as L23767 and referred herein as SEQ ID NO: 3. a2,6-Sialyltransferase - The coding region of the gene for beta-galactosamide alpha- 2,6-sialyltransferase 1 (a2,6-sialyltransferase, ST6GAL1 ) was used according to Grundmann et al. (1990). The sequence is banked as NM_003032 and referred herein as SEQ ID NO: 4.
EXAMPLES
Example 1 Construction of the FSH expression vector
The coding sequence of FSH alpha polypeptide (AH007338, SEQ ID NO: 1 ) and FSH beta polypeptide (NM_003032, SEQ ID NO: 2) were amplified by PCR using the primer combinations FSHa-fw and FSHa-rev and FSHb-fw and FSHb-rec respectively.
FSHa-fw 5'-CCAGGATCCGCCACCATGGATTACTACAGAAAAATATGC-3' (SEQ ID NO:9) FSHa-rev 5'-GGATGGCTAGCTTAAGATTTGTGATAATAAC-3' (SEQ ID NO:10)
FSHb-fw 5'-CCAGGCGCGCCACCATGAAGACACTCCAGTTTTTC-3' (SEQ ID NO: 1 1 ) FSHb-rev 5'-CCGGGTTAACTTATTATTCTTTCATTTCACCAAAGG-3' (SEQ ID NO: 12)
The resulting amplified FSH beta DNA was digested with the restriction enzymes Asc\ and Hpa\ and inserted into the Asc\ and Hpa\ sites on the CMV driven mammalian expression vector carrying a neomycin selection marker. Similarly the FSH alpha DNA was digested with SamHI and Nhe\ and inserted into the sites SamHI and Nhe\ on the expression vector already containing the FSH beta polypeptide DNA.
The vector DNA was used to transform the DH5a strain of E.coli. Colonies were picked for amplification. Colonies containing the vector containing both FSH alpha and beta were selected for sequencing and all contained the correct sequences according to SEQ ID NO: 1 and SEQ ID NO: 2. Plasmid pFSH A+B#17 was selected for transfection (Figure 1 ).
Example 2 Construction of the ST3 expression vector
The coding sequence of beta-galactoside alpha-2,3-sialyltransferase 4 (ST3, L23767,
SEQ ID NO: 3) was amplified by PCR using the primer combination 2,3STfw and 2,3STrev.
2,3STfw 5'-CCAGGATCCGCCACCATGTGTCCTGCAGGCTGGAAGC-3' (SEQ ID NO: 13) 2,3STrev 5'-TTTTTTTCTTAAGTCAGAAGGACGTGAGGTTCTTG-3' (SEQ ID NO: 14)
The resulting amplified ST3 DNA was digested with the restriction enzymes BamH\ and AflW and inserted into the BamH\ and AflW sites on the CMV driven mammalian expression vector carrying a hygromycin resistance marker. The vector was amplified as previously described and sequenced. Clone pST3#1 (Figure 2) contained the correct sequence according SEQ ID NO: 3 and was selected for transfection.
Example 3 Construction of the ST6 expression vector
The coding sequence of beta-galactosamide alpha-2,6-sialyltransferase 1 (ST6,
NM_003032, SEQ ID NO: 4) was amplified by PCR using the primer combination 2,6STfw and 2,6STrev.
2,6STfw 5'-CCAGGATCCGCCACCATGATTCACACCAACCTGAAG-3' (SEQ ID NO: 15) 2,6STrev 5'-TTTTTTTCTTAAGTTAGCAGTGAATGGTCCGG-3' (SEQ ID NO: 16)
The resulting amplified ST6 DNA was digested with the restriction enzymes BamH\ and AflW and inserted into the BamH\ and AflW sites on the CMV driven mammalian expression vector carrying a hygromycin resistance marker. The vector was amplified as previously described and sequenced. Clone pST6#1 1 (Figure 3) contained the correct sequence according SEQ ID NO: 4 and was selected for transfection.
Example 4 Stable expression of pFSH α+β in PER.C6® cells. Transfection isolation and screening of clones.
PER.C6®clones producing FSH were generated by expressing both polypeptide chains of FSH from a single plasmid (see Example 1 ).
To obtain stable clones a liposome based transfection agent with the pFSH α+β construct. Stable clones were selected in VPRO supplemented with 10% FCS and containing G418. Three weeks after transfection G418 resistant clones grew out. Clones were selected for isolation. The isolated clones were cultured in selection medium until 70-80% confluent. Supernatants were assayed for FSH protein content using an FSH selective ELISA and pharmacological activity at the FSH receptor in cloned cell line, using a cAMP accumulation assay. Clones expressing functional protein were progressed for culture expansion to 24 well, 6 well and T80 flasks.
Studies to determine productivity and quality of the material from seven clones were initiated in T80 flasks to generate sufficient material. Cells were cultured in supplemented media as previously described for 7 days and the supernatant harvested. Productivity was determined using the FSH selective ELISA. The isoelectric profile of the material was determined by Isoelectric focusing (IEF), by methods known in the art. Clones with sufficient productivity and quality were selected for sialyltransferase engineering. Example 5 Level of sialylation is increased in cells that over express a2,3- sialyltransferase. Stable expression of pST3 in FSH expressing PER.C6® cells; Transfection isolation and screening of clones.
PER.C6® clones producing highly sialylated FSH were generated by expressing a2,3 sialyltransferase from separate plasmids (Example 2) in PER.C6® cells already expressing both polypeptide chains of FSH (from Example 4). Clones produced from PER.C6® cells as set out in Example 4 were selected for their characteristics including productivity, good growth profile, production of functional protein, and produced FSH which included some sialylation. Stable clones were generated as previously described in Example 4. Clones were isolated, expanded and assayed. The a2,3-sialyltransferase clones were adapted to serum free media and suspension conditions.
As before, clones were assayed using a FSH selective ELISA, functional response in an FSH receptor cell line, IEF, metabolic clearance rate and Steelman Pohley analysis. Results were compared to a commercially available recombinant FSH (Gonal-f, Serono) and the parental FSH PER.C6®cell lines. FSH produced by most of the clones has significantly improved sialylation (i.e. on average more FSH isoforms with high numbers of sialic acids) compared to FSH expressed without a2,3- sialyltransferase. In conclusion expression of FSH together with sialyltransferase in PER.C6®cells resulted in increased levels of sialylated FSH compared to cells expressing FSH only. Example 6 Production and purification overview
A procedure was developed to produce FSH in PER.C6®cells that were cultured in suspension in serum free medium. The procedure is described below and was applied to several FSH-producing PER.C6®cell lines.
FSH from a2,3- clone (Example 5) was prepared using a using a modification of the method described by Lowry et al. (1976).
For the production of PER.C6®-FSH, the cell lines were adapted to a serum- free medium, i.e., Excell 525 (JRH Biosciences). The cells were first cultured to form a 70%-90% confluent monolayer in a T80 culture flask. On passage the cells were re-suspended in the serum free medium, Excell 525 + 4 rtiM L-Glutamine, to a cell density of 0.3x106 cells/ml. A 25 ml cell suspension was put in a 250 ml shaker flask and shaken at 100 rpm at 37°C at 5% CO2. After reaching a cell density of > IxlO6 cells/ml, the cells were sub-cultured to a cell density of 0.2 or 0.3x106 cells/ml and further cultured in shaker flasks at 37°C, 5% C02 and 100 rpm. For the production of FSH, the cells were transferred to a serum- free production medium, i.e., VPRO (JRH Biosciences), which supports the growth of PER.C6®cells to very high cell densities (usually > 107 cells/ml in a batch culture). The cells were first cultured to > 1 xlO6 cells/ml in Excell 525, then spun down for 5 min at 1000 rpm and subsequently suspended in VPRO medium + 6 mM L-glutamine to a density of 1x106 cells/ml. The cells were then cultured in a shaker flask for 7-10 days at 37°C, 5% CO2 and 100 rpm. During this period, the cells grew to a density of > 107 cells/ml. The culture medium was harvested after the cell viability started to decline. The cells were spun down for 5 min at 1000 rpm and the supernatant was used for the quantification and purification of FSH. The concentration of FSH was determined using ELISA (DRG EIA 1288).
Thereafter, purification of FSH was carried out using a modification of the method described by Lowry et al. (1976). Purification using charge selective chromatography was carried out to enrich the highly sialylated forms by methods well known in the art.
During all chromatographic procedures, enrichment of the sialylated forms of FSH as claimed herein was confirmed by RIA (DRG EIA 1288) and/or IEF.
Example 7 Quantification of relative amounts of a2,3 and a2,6 sialic acid
The relative percentage amounts of a2,3 and a2,6 sialic acid on purified rFSH (Example 6) were measured using known techniques.
N-Glycans were released from the samples using PNGase F under denaturative conditions and then labelled with 2-aminobenzamide. Released glycan forms were then separated and analysed by Weak Anion Exchange (WAX) column for determination of charge distribution. Labelled glycans treated with 2,3,6,8 sialidase for determination of total sialic acid and 2,3 sialidase for determination of 2,3 sialic acid, were further analyzed by wax column.
The relative percentages of the charged glycans were calculated from structures present in the undigested and digested glycan pools and are shown in Figure 4 (for 8 samples). These were found to be in the ranges 50% - 70% (e.g. about 60% or 65%) for a2,3 sialylation and 28 to 50%, generally 30 to 35% (e.g. about 31 % or 35%), for a2,6 sialylation. Example 8 Quantification of relative amounts mono, di, tri and tetra sialylated glycan structures
The relative percentage amounts of mono, di, tri and tetra sialylated structures on glycans extracted from purified rFSH (Example 6) were measured using known techniques.
N Glycans were released from the samples using PNGase F under denaturative conditions and then were labeled with 2-aminobenzamide. Glycans were released from the samples using PNGase F under denaturative conditions and then labeled with 2- aminobenzamide. Released glycan forms were then separated and analysed by Weak Anion Exchange (WAX) column for determination of sialylation distribution. The relative amounts of neutral, mono-sialylated, di-sialylated, tri-sialylated and tetra-sialylated structures are shown in Figure 5 (for the 8 samples shown in Fig 4).
The rFSH includes neutral , mono-sialylated, di- sialylated, tri- sialylated and tetra- sialylated glycan structures with relative amounts as follows: neutral 5-6 %; 15-17% mono- sialylated; 26-30% di-sialylated; 30-32% tri-sialylated and 17-23 % tetra-sialylated.
Example 8a
The relative percentage amounts of a2,6 sialic acid on purified rFSH extracted from nine samples of purified rFSH (produced by the methods of Example 6) were measured using known techniques.
N-Glycans were released from the samples using PNGase F under denaturative conditions and then labelled with 2-aminobenzamide. Released glycan forms were then separated and analysed by Weak Anion Exchange (WAX) column for determination of charge distribution. Labelled glycans treated with 2,3,6,8 sialidase for determination of total sialic acid and 2,3 sialidase for determination of 2,3 sialic acid, were further analyzed by wax column (see Example 8). The analysis allows calculation of a2,6 sialic acid.
The relative percentages of the charged glycans were calculated from structures present in the undigested and digested glycan pools and are shown in the following Table. These were found to be in the ranges 25 to 50%, generally 30 to 35% for a2,6 sialylation.
The relative percentage amounts of bisecting GlcNac, GalNac and 1 -Fucose Lewis on glycans extracted from the nine samples of purified rFSH (produced by the methods of Example 6) were measured using known techniques. N-Glycans were released from the glycoprotrein using PNGase F and labeled with 2-aminobenzamide (2AB). The analysis was done by two dimensional (2D) HPLC analysis in combination with enzymatic degradation of the glycans. For verification, the glycans were analyzed by MALDI-MS The relative amounts of alpha 2,6-sialic acid and the terminal residues are shown in the following table, together with those for Gonal F (CHO cell derived recombinant FSH) and Bravelle (human urinary FSH).
Figure imgf000024_0001
I GalNA 0 1 1.3
51 44.6 50.7 44.7 49 47.6 45.3 46.4 44.9 47.1
c
Bisectin
g 10
GlcNAc 12.4 10.2 8.9 8.7 1 1.8 1 1.4 10.6 13.9 10.9 55 14
1
Fucose 21.1 16.7 23.3 16.1 20.3 18.1 17.9 18.7 19.0 19.0 3.11 2.2 Lewis
2
Fucose 4 4.1 4.3 1.9 3.1 4.2 3.8 3.9 4.4 3.7 - n.d.2 Lewis
Value of 3.1 is total ½ Fucose Lewis. Not determined.
It can be seen that the amount of GalNac in the FSH of the invention varies between about 44.9 and 51 %, averaging about 47.1 %.
It can be seen that the amount of bisecting GlcNac in the FSH of the invention varies between 8.7 and 13.9%, averaging approximately at 10.9%.
It can be seen that the amount of 1 Fucose Lewis in the FSH of the invention varies between 16.1 and 23.3%, averaging approximately at 19%.
It can be seen that the amount of 2 Fucose Lewis in the FSH of the invention varies between 1 .9 and 4.4%, averaging approximately at 3.7%.
Example 9 - A multiple dose study investigating the safety, tolerability, pharmacokinetics, pharmacodynamics, and immunogenicity of FE 999049 in comparison to GONAL-F.
Study population
A total of 48 (24 on each drug) healthy women received daily doses of 14.6 μg of FE 999049 (a composition according to the invention, produced according to Example 6) or 16.5 μg of Gonal- F for seven days.
Safety results
Multiple dose administration of FE 999049 and GONAL-F was safe and generally well tolerated as assessed by Adverse Events (AEs), vital signs, ECG, clinical laboratory measurements, and physical examination. No serious adverse event or death occurred during the study.
Pharmacokinetic results
Following the administration of FE 999049 and GONAL-F over 7 days, the FSH concentration values as assessed immediately prior to the next injection increased and seemed to reach a steady state level after 6-7 days. However the exposure (AUC and Cmax) of FE 999049 was 60% higher in comparison to Gonal-F.
Pharmacodynamic results
The concentrations of inhibin-B (see figure 6), oestradiol, and progesterone all increased subsequent to administration of FE 999049 and GONAL-F, however to a greater extent following administration of FE 999049 compared to GONAL-F. Both number and size distribution of follicles showed a greater response to FE 999049 compared to GONAL-F. Example 9 demonstrates that FSH having a specific amount (17-23%) of tetra-sialylated glycan structures and e.g. specific amounts of a2,3 sialylation and a2,6 sialylation is markedly more potent then recombinant FSH products which are currently on the market.
Example 10 - A multiple dose study investigating FE 999049 in comparison to GONAL-F.
The following describes a randomised, controlled, assessor-blind, parallel groups, multinational, multicentre trial assessing the dose-response relationship of FE 999049 in patients undergoing controlled ovarian stimulation for in vitro fertilisation (IVF) / intracytoplasmic sperm injection (ICSI). The patient population was 265 IVF patients aged between 18 to 37 years, with BMI 18.5 to 32.0 kg/m2.
The trial was designed as a dose-response trial with number of oocytes retrieved as the primary endpoint. Secondary endpoints will explore the qualitative and quantitative impact of different doses of FE 999049 with regard to endocrine profile, follicular development, oocyte fertilisation, embryo quality and treatment efficiency (i.e. total gonadotropin consumption and duration of stimulation). The trial is designed to evaluate the efficacy of FE 999049 to establish pregnancy when used in controlled ovarian stimulation for IVF/ICSI cycles.
Subjects were assessed within 3 months prior to randomisation for compliance with the inclusion and exclusion criteria, including an anti-Mijllerian hormone (AMH) assessment to increase homogeneity of the trial population in relation to ovarian response and minimise the number of potential poor and hyper-responders to the FE 999049 doses and GONAL-F dose used in the trial. The AMH assessment was measured using the AMH Gen-ll enzyme linked immunosorbent assay kit (Beckman Coulter, Inc., Webster, Texas). This assay can detect AMH concentrations greater than 0.57 pmol/L with a minimum limit of quantitation of 1 .1 pmol/L.
On day 2-3 of their menstrual cycle, subjects were randomised in a 1 : 1 :1 : 1 :1 : 1 fashion to treatment with either 90 IU, 120 IU, 150 IU, 180 IU or 210 IU FE 999049 or 150 IU GONAL-F, and ovarian stimulation initiated. Randomisation was stratified according to AMH level at screening [5.0-14.9 pmol/L (low AMH) and 15.0 to 44.9 pmol/L (high AMH)).
Gonal-F is filled by mass (FbM) at FDA request; referring to μg dose is therefore appropriate. The Gonal -F label indicates 600 IU/44 μg, which indicates that 150 IU is 1 1 μg. However, there is some variation and the batch certificate for this trial indicated that 11.3 pg Gonal-F was equivalent to 150 IU. The FE999049 doses are presented by protein content (pg) rather than biological activity. Thus the doses of FE999049 were 5.2pg (90 IU), 6.9pg (120 IU), 8.6pg (150 IU), 10.3pg (180 IU) or 12.1 pg (210 IU).
The subject and dose distribution is set out as follows (data are number of subjects):
Table 1
FE 999049 GONAL-F
5.2pg 6.9pg 8.6pg 10.3pg 12.1 μς 1 1.3 (1 1 ) Mg Total
Screened 334
Randomised
42 45 44 45 46 43 265 and exposed
High AMH
strata
23 26 24 24 26 25 148 (56%)
(15.0 - 44.9
pmol/L)
Low AMH
strata
19 19 20 20 21 18 1 17 (44%)
(5.0 - 14.9
pmol/L)
Per-protocol 40 42 42 44 44 43 255
The daily dose level of FE 999049 or GONAL-F is fixed throughout the entire stimulation period. During stimulation, subjects are monitored on stimulation day 1 , 4 and 6 and hereafter at least every second day. When 3 follicles of >15 mm are observed, visits are performed daily. Subjects are treated with FE 999049 or GONAL-F for a maximum of 16 days.
To prevent a premature LH surge, a GnRH antagonist (ganirelix acetate,
ORGALUTRAN, MSD / Schering-Plough) may be initiated on stimulation day 6 at a daily dose of 0.25 mg and continued throughout the stimulation period. Triggering of final follicular maturation is done on the day when >3 follicles with a diameter >17 mm are observed. If there are <25 follicles with a diameter >12 mm, 250 pg recombinant hCG (choriogonadotropin alfa, OVITRELLE, Merck Serono / EMD Serono) is administered. If there are 25-35 follicles with a diameter≥12 mm, 0.2 mg GnRH agonist (triptorelin acetate, DECAPEPTYL / GONAPEPTYL, Ferring Pharmaceuticals) is administered. In case of excessive ovarian response, defined as >35 follicles with a diameter >12 mm, the treatment is cancelled. In case of poor ovarian response, defined as <3 follicles with a diameter >10 mm observed on stimulation day 10, the cycle could be cancelled.
Oocyte retrieval takes place 36h (± 2h) after triggering of final follicular maturation and the oocytes inseminated by IVF and/or ICSI. Fertilisation and embryo development are assessed from oocyte retrieval to the day of transfer. For subjects who underwent triggering of final follicular maturation with hCG, one blastocyst of the best quality available is transferred on day 5 after oocyte retrieval while remaining blastocysts are frozen. For subjects who undergo triggering of final follicular maturation with GnRH agonist, no embryo transfer takes place in the fresh cycle and blastocysts are instead frozen on day 5. Vaginal progesterone tablets (LUTINUS, Ferring Pharmaceuticals) 100 mg 3 times daily are provided for luteal phase support from the day after oocyte retrieval until the day of the clinical pregnancy visit. A βΙιΟΘ test is performed 13-15 days after embryo transfer and clinical pregnancy will be confirmed by transvaginal ultrasound (TVU) 5-6 weeks after embryo transfer.
Results
The number of oocytes retrieved (primary endpoint) is shown in the following Table.
Table 2
FE 999049 GONAL-F
5.2 pg 6.9 pg 8.6 pg 10.3 pg 12.1 g 11.3 (11 )ug
Oocytes
retrieved
All 5.2 (3.3) 7.9 (5.9) 9.2 (4.6) 10.6 (7.0) 12.2 (5.9) 10.4 (5.2)
High AMH 5.9 (3.9) 9.1 (6.4) 10.6 (4.8) 13.6 (7.8) 14.4 (5.8) 12.4 (5.4)
Low AMH 4.5 (2.2) 6.3 (4.9) 7.4 (3.8) 6.9 (3.6) 9.4 (4.9) 7.8 (3.4)
Data are mean (SD)
The primary objective was met: a significant dose-response relationship was established for FE 999049 with respect to number of oocytes retrieved. This finding was observed not only for the overall trial population, but also for each of the two AMH strata used at randomisation. A significant dose-response for FE 999049 was demonstrated for all key objective pharmacodynamic parameters, e.g. estradiol, inhibin B and inhibin A. At a similar microgram dose level, the pharmacodynamic responses with FE 999049 were larger than with GONAL-F (these results not shown).
The serum FSH concentrations after exposure to FE 999049 were significantly higher than for GONAL-F. The results confirm that the PK profile of FE 999049 differs from that of GONAL-F. Fertilisation rates, blastocyst development and pregnancy rates in IVF/ICSI patients treated with FE 999049 were within expectations.
There were no safety concerns with the use of FE 999049. A good local tolerability was documented.
Further Analysis
The applicants have further analysed the data to identify the FE 999049 dose(s) that fulfil the following criteria with respect to number of oocytes retrieved:
· Oocytes retrieved in the range 8-14
Minimise proportion of patients with <8 oocytes
Minimise proportion of patients with≥20 oocytes
The applicants also investigated the impact of body weight. If relevant, the dose is converted into μg/kg for an average subject. This value of pg/kg and ±0.01 g kg are evaluated in a model with respect to distribution of oocytes retrieved as well as safety profile, and the optimal dose is identified.
Low AMH strata
As seen in Table 2, the dose of FE999049 which fulfilled the first criterion (Oocytes retrieved in the range 8-14) was 12.1 g (mean 9.4 oocytes retrieved). The distribution of oocytes is shown in Table 3 below.
Table 3
FE 999049 GONAL-F 5.2pg 6^g 8.6pg 10.3pg 12.1 pg 1 1 .3 (1 1 )pg
Oocytes
retrieved
<4 32% 24% 15% 10% 10% 6% 4-7 63% 42% 45% 60% 20% 56%
8-14 5% 24% 35% 30% 60% 33%
15-19 0% 5% 5% 0% 5% 6%
>20 0% 5% 0% 0% 5% 0%
Data are % of subjects
As shown by the box and arrow, a dose of 12.1 pg FE999049 provides retrieval of the most desirable number of oocytes in 60% of subjects in the low AMH group. This is a marked improvement on Gonal-F (most desirable number of oocytes in only 33% of subjects).
Table 4 below shows the analysis of signs of excessive response in the low AMH strata (data are number of subjects). It can be seen that there were no indications of early OHSS of a moderate or severe nature and there were no incidences of preventative action being required; there are no concerns associated with the dose of 12.1 pg FE999049 in a patient having low AMH.
Table 4
FE 999049 GONAL-F
5.2pg 6.9pg 8.6pg 10.3pg 12.1 pg 11.3(11)pg
All subjects 19 19 20 20 21 18
Early OHSS, mod/sev
GnRH agonist
triggering
Preventive action*
≥15 oocytes
Any of the above Fig 7 shows the effect of body weight on oocytes retrieved (for the low AMH strata), for the various doses. The arrows indicate the number of oocytes retrieved from subjects of bodyweight between 45kg and 90kg treated at the 12.1 μg dose. As can be seen (text box) the variation between patients of bodyweight 45kg and those of 90kg is less than around 0.5 oocytes; in other words dosing by body weight is not required in patients with low AMH when dose of FE999049 is at least 12 μg, because there is not a significant variation in oocytes retrieved with body weight, at this dose.
Thus the applicants have found that a dose of, or dose equivalent to, 6 to 18 μg, for example 9 to 14 μg, for example 12 μg, human derived recombinant FSH is suitable for use in the treatment of infertility in a patient having serum AMH <15 pmol/L, for example 0.05-14.9 pmol/L for example 5.0-14.9 pmol/L. The dose provides an effective response while minimising risk of OHSS.
High AMH strata
As seen in Table 2, three doses of FE999049 fulfilled the first criterion (oocytes retrieved in the range 8-14): 6.9 μg (mean 9.1 oocytes retrieved), 8.6 μg (mean 10.6 oocytes retrieved), and 10.3 μg (mean 13.6 oocytes retrieved).
Fig 8 shows the effect of body weight on oocytes retrieved (for the high AMH strata), for the various doses. The arrows indicate the number of oocytes retrieved from subjects of body weight between 45kg and 90kg treated at the 6.9 μg, 8.6 μg and 10.3 μg doses. As can be seen (text boxes) the variation is significant: for the 6.9 μg dose 6 additional oocytes will be retrieved from a 45 kg patient compared to a 90 kg patient; for the 8.6 μg dose 4 additional oocytes will be retrieved from a 45 kg patient compared to a 90 kg patient; and for the 10.1 μg dose 2.5 additional oocytes will be retrieved from a 45 kg patient compared to a 90 kg patient. In other words dosing by body weight has an impact in patients with high AMH when the dose of FE999049 is less than 12 μg, because there is a significant variation in oocytes retrieved with body weight, at these doses.
Table 5a below shows a further breakdown of oocytes retrieved (from Table 2) by AMH. This shows the doses which fulfilled the first criterion (oocytes retrieved in the range 8-14) for each sub strata of AMH (boxes).
Figure imgf000031_0001
5.2 pg 6.9 pg 8.6 pg 10.3 pg 12.1 pg
Oocytes retrieved
15-24 pmol/L 4.9 (3.8) 7.3 (3.6) 10.6 (5.1) 11.5 (6.7) 12.3 (5.9)
25-34 pmol/L 7.0 (1.8) 9.1 (6.8) 9.7 (6.7) 15.5 (6.4) 16.7 (4.9)
35-45 pmol/L 8.5 (9.2) \ 21.0 (1.4) \11.3 (2.6) \ 18.0 15.7 (6.5)
(12.2)
Table 5 b below shows the analysis of patients where treatment was cancelled due to either excessive response or agonist triggering, for these subgroups. For example, one patient in the 25-34 pmol/L AMH strata cancelled due to excessive response following the dose of 10.3 pg and one patient in the 25-34 pmol/L AMH strata cancelled due to excessive response following the dose of 12.1 pg; one patient in the 35-45 pmol/L AMH strata cancelled following agonist triggering following dose of 10.3 pg; and one patient in the 35-45 pmol/L AMH strata cancelled following agonist triggering following dose of 6.9 pg.
Table 5b
FE 999049
5.2pg 6.9pg 8.6pg 10.3pg 12.1 pg
OHSS***, cancellation
due to excessive
response or agonist
triggering****
15-24 pmol/L 0 25-34 pmol/L 0 35-45 pmol/L 0 It can be seen therefore that tailoring of dose by bodyweight (Fig 8) and AMH level would be useful in the high AMH strata, to minimise cancellations and maximise oocyte retrieval.
The applicants have found that the following doses provide an effective response while minimising risk of OHSS (kg is kg body weight of patient).
Figure imgf000033_0001
The following are appropriate if dosing by bodyweight is not desired.
Figure imgf000033_0002
The following are appropriate if fewer categories of AMH are required. AMH categories 3 AMH categories 2 AMH categories One dose
AMH Dose AMH Dose AMH Dose AMH Dose
<15 12 pg <15 12 pg <15 12 pg - 0.16 pg/kg
15-24 0.15-0.16 pg/kg 15-24 0.15-0.16 ≥15 0.14 pg/kg
pg/kg
25-34 0.12-0.13 pg/kg >25 0.12 pg/kg ≥35 0.10-0.11 pg/kg The following are appropriate if dosing by bodyweight is not desired. .
4 AMH categories 3 AMH categories 2 AMH categories One dose
AMH Dose AMH Dose AMH Dose AMH Dose
<15 12 pg <15 12 pg <15 12 pg - 9.3 pg or 10 pg
15-24 9.3-10 pg 15-24 9.3-10 pg ≥15 8.7 pg
25-34 7.3-8 pg >25 7.3 pg
>35 6.3-7 pg
Thus the applicants have found that a dose of, or dose equivalent to, 9 to 14 pg, for example 12 pg, human derived recombinant FSH is suitable for use in the treatment of infertility in a patient having serum AMH <15 pmol/L, for example 0.05-14.9 pmol/L for example 5.0-14.9 pmol/L. The dose provides an effective response while minimising risk of OHSS. The applicants have found that a dose of, or dose equivalent to, 5 to 12.5 pg, for example 6 to 10.5 pg, human derived recombinant FSH is suitable for use in the treatment of infertility in a patient having serum AMH≥ 5 pmol/L. The dose provides an effective response while minimising risk of OHSS.
The applicants have found that a (e.g. daily) dose of, or dose equivalent to, 0.09 to 0.19 pg human derived recombinant FSH per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of≥15 pmol/L. The applicants have found that a (e.g. daily) dose of, or dose equivalent to, 0.14 to 0.19 pg human derived recombinant FSH (preferably 0.15 to 0.16 pg human derived recombinant FSH) per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of 15 to 24.9 pmol/L. The applicants have found that a (e.g. daily) dose of, or dose equivalent to, 0.1 1 to 0.14 pg human derived recombinant FSH (preferably 0.12 to 0.13 pg human derived recombinant FSH) per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of 25 to 34.9 pmol/L. The applicants have found that a (e.g. daily) dose of, or dose equivalent to, 0.10 to 0.1 1 μg human derived recombinant FSH per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of≥ 35 pmol/L. These doses provide an effective response while minimising risk of OHSS.
The applicants have found that a (e.g. daily) dose of, or dose equivalent to, 0.15 to 0.21 μg (e.g. 0.16 μg) human derived recombinant FSH per kg bodyweight of the patient is suitable for use in the treatment of infertility in a patient having serum AMH level of <15 pmol/L, for example for the first stimulation cycle with human derived recombinant FSH. . However, it is not required that patients are dosed by body weight at this level of AMH.
Example 10 A - Individualised COS protocol (low AMH)
The selected patients are about to undergo COS for in vitro fertilisation (IVF) / intracytoplasmic sperm injection (ICSI) by methods known in the art. The pre-treatment protocol includes assessment/screening of the patient's serum AMH using the AMH Gen-ll enzyme linked immunosorbent assay kit (Beckman Coulter, Inc., Webster, Texas). This assay can detect AMH concentrations greater than 0.57 pmol/L with a minimum limit of quantitation of 1.1 pmol/L. AMH may be measured using other Assay kits (e.g. available from Roche).
The COS protocol proceeds in the usual manner apart from administration of the initial dose of FE 999049 according to AMH level at screening. A patient with an AMH level of <14.9 pmol/L would be administered an initial daily dose of approximately 12 μg FE 999049, a human derived recombinant FSH product manufactured according to the method of Example 6. A patient with an AMH level of 15 to 24.9 pmol/L would receive an initial daily dose of 0.15 to 0.19 μg of the human derived recombinant FSH per kg bodyweight of the patient. A patient with an AMH level of 25 to 34.9 pmol/L would receive an initial daily dose of 0.1 1 to 0.13 μg of the human derived recombinant FSH per kg bodyweight of the patient. A patient with an AMH level of ≥ 35 pmol/L would receive an initial daily dose of 0.10 to 0.1 1 μg of the human derived recombinant FSH per kg bodyweight of the patient.
Example 11 - Individualised COS protocols.
The doses in this protocol are less preferred that Example 10A.
The selected patients are about to undergo COS for in vitro fertilisation (IVF) / intracytoplasmic sperm injection (ICSI) by methods known in the art. The pre-treatment protocol includes assessment/screening of the patient's serum AMH using the AMH Gen-ll enzyme linked immunosorbent assay kit (Beckman Coulter, Inc., Webster, Texas). This assay can detect AMH concentrations greater than 0.57 pmol/L with a minimum limit of quantitation of 1.1 pmol/L.
The COS protocol proceeds in the usual manner apart from administration of the initial dose of FE 999049 according to AMH level at screening in line with the following table. Thus a patient with an AMH level of 5-14.8 pmol/L would be administered 180 IU FSH in the form of approximately 8-1 1 μg FE 999049, a human derived recombinant FSH product manufactured according to the method of Example 6. A patient with an AMH level of 30-44.9 pmol/L would be administered 120 I U FSH in the form of approximately 4-7 μg FE 999049, a human derived recombinant FSH product manufactured according to the method of Example 6. If the AMH level is not available, the patient recombinant would be administered 120-180 IU FSH in the form of approximately 6-1 1 μg FE 999049, a human derived recombinant FSH product manufactured according to the method of Example 6.
Figure imgf000036_0001
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Ulloa-Aguirre A, Maldonado A, Damian-Matsumura P, and Timossi C (2001 ). Endocrine regulation of gonadotropin glycosylation. Arch Med Res. 32(6), 520-532. Ulloa-Aguirre A, Timossi C, Barrios-de-Tomasi J, Maldonado A, and Nayudu P. (2003). Impact of carbohydrate heterogeneity in function of follicle-stimulating hormone: studies derived from in vitro and in vivo models. Biol Reprod. 69(2), 379-389.
Van Lenten L, and Ashwell G. (1972) The binding of desialylated glycoproteins by plasma membranes of rat liver. Development of a quantitative inhibition assay. J Biol Chem. 247(14), 4633- 40.
Wide, L. and Albertsson-Wikland, K. (1990) Change in electrophoretic mobility of human follicle- stimulating hormone in serum after administration of gonadotropin-releasing hormone. J. Clin. Endocrinol. Metab. 70, 271-276.
Wide, L. and Bakos, O. (1993). More basic forms of both human follicle-stimulating hormone and luteinizing hormone in serum at midcycle compared with the follicular or luteal phase. J. Clin. Endocrinol. Metab., 76^ 885-889.
Wide L, Naessen T, Sundstrom-Poromaa I, Eriksson K. (2007) Sulfonation and sialylation of gonadotropins in women during the menstrual cycle, after menopause, and with polycystic ovarian syndrome and in men. J Clin Endocrinol Metab. ;92(11), 4410-4417.
Zambrano E, Zarinan T, Olivares A, Barrios-de-Tomasi J, and Ulloa-Aguirre A. (1999). Receptor binding activity and in vitro biological activity of the human FSH charge isoforms as disclosed by heterologous and homologous assay systems: implications for the structure-function relationship of the FSH variants. Endocrine. 10(2), 113-121. Zhang X, Lok SH, and Kon OL (1998) Stable expression of human alpha-2,6-sialyltransferase in Chinese hamster ovary cells: functional consequences for human erythropoietin expression and bioactivity. Biochim Biophys Acta. 1425(3). 441-452. Figs 1 , 2 and 3: Plasmid maps of the pFSHalpha/beta ,pST3 and pST6 expression vectors. CMV = Cytomegalovirus promoter, BGHp(A) = Bovine Growth Hormone poly-adenylation sequence, fl ori = fl origin of replication, SV40 = Simian Virus 40 promoter, Neo = Neomycin resistance marker, Hyg = Hygromycin resistance marker, SV40 p(A) = Simian Virus 40 poly-adenylation sequence, FSH A = Follicle stimulating hormone alpha polypeptide, FSH B = Follicle stimulating hormone beta polypeptide, ST3GAL4 = a2,3- sialyltransferase, ST6GAL1 = a2,6-sialyltransferase, ColEI = ColEI origin of replication, Amp = ampicillin resistance marker.
SEQ ID NO: 1
Follicle stimulating hormone alpha polypeptide
Accession number AH007338
Nucleotide sequence of FSH alpha
1 ATGGATTACT ACAGAAAATA TGCAGCTATC TTTCTGGTCA CATTGTCGGT GTTTCTGCAT 61 GTTCTCCATT CCGCTCCTGA TGTGCAGGAT TGCCCAGAAT GCACGCTACA GGAAAACCCA 121 TTCTTCTCCC AGCCGGGTGC CCCAATACTT CAGTGCATGG GCTGCTGCTT CTCTAGAGCA 181 TATCCCACTC CACTAAGGTC CAAGAAGACG ATGTTGGTCC AAAAGAACGT CACCTCAGAG 241 TCCACTTGCT GTGTAGCTAA ATCATATAAC AGGGTCACAG TAATGGGGGG TTTCAAAGTG 301 GAGAACCACA CGGCGTGCCA CTGCAGTACT TGTTATTATC ACAAATCTTA A
Protein sequence of FSH alpha (SEQ ID NO: 5)
1 MDYYRKYAAI FLVTLSVFLH VLHSAPDVQD CPECTLQENP FFSQPGAPIL QCMGCCFSRA 61 YPTPLRSKKT MLVQKNVTSE STCCVAKSYN RVTVMGGFKV ENHTACHCST CYYHKS
SEQ ID NO: 2
Follicle stimulating hormone beta polypeptide
Accession number NM_000510 Nucleotide sequence of FSH beta
1 ATGAAGACAC TCCAGTTTTT CTTCCTTTTC TGTTGCTGGA AAGCAATCTG CTGCAATAGC 61 TGTGAGCTGA CCAACATCAC CATTGCAATA GAGAAAGAAG AATGTCGTTT CTGCATAAGC 121 ATCAACACCA CTTGGTGTGC TGGCTACTGC TACACCAGGG ATCTGGTGTA TAAGGACCCA 181 GCCAGGCCCA AAATCCAGAA AACATGTACC TTCAAGGAAC TGGTATATGA AACAGTGAGA 241 GTGCCCGGCT GTGCTCACCA TGCAGATTCC TTGTATACAT ACCCAGTGGC CACCCAGTGT 301 CACTGTGGCA AGTGTGACAG CGACAGCACT GATTGTACTG TGCGAGGCCT GGGGCCCAGC 361 TACTGCTCCT TTGGTGAAAT GAAAGAATAA
Protein sequence of FSH beta (SEQ ID NO: 6)
1 MKTLQFFFLF CCWKAICCNS CELTNITIAI EKEECRFCIS INTTWCAGYC YTRDLVYKDP 61 ARPKIQKTCT FKELVYETVR VPGCAHHADS LYTYPVATQC HCGKCDSDST DCTVRGLGPS 121 YCSFGEMKE SEQ ID NO: 3
Beta-galactoside alpha-2,3-sialyltransferase 4
Accession Number L23767
Nucleotide sequence of ST3GAL4
1 ATGTGTCCTG CAGGCTGGAA GCTCCTGGCC ATGTTGGCTC TGGTCCTGGT CGTCATGGTG
61 TGGTATTCCA TCTCCCGGGA AGACAGGTAC ATCGAGCTTT TTTATTTTCC CATCCCAGAG
121 AAGAAGGAGC CGTGCCTCCA GGGTGAGGCA GAGAGCAAGG CCTCTAAGCT CTTTGGCAAC
181 TACTCCCGGG ATCAGCCCAT CTTCCTGCGG CTTGAGGATT ATTTCTGGGT CAAGACGCCA
241 TCTGCTTACG AGCTGCCCTA TGGGACCAAG GGGAGTGAGG ATCTGCTCCT CCGGGTGCTA
301 GCCATCACCA GCTCCTCCAT CCCCAAGAAC ATCCAGAGCC TCAGGTGCCG CCGCTGTGTG
361 GTCGTGGGGA ACGGGCACCG GCTGCGGAAC AGCTCACTGG GAGATGCCAT CAACAAGTAC
421 GATGTGGTCA TCAGATTGAA CAATGCCCCA GTGGCTGGCT ATGAGGGTGA CGTGGGCTCC
481 AAGACCACCA TGCGTCTCTT CTACCCTGAA TCTGCCCACT TCGACCCCAA AGTAGAAAAC
541 AACCCAGACA CACTCCTCGT CCTGGTAGCT TTCAAGGCAA TGGACTTCCA CTGGATTGAG
601 ACCATCCTGA GTGATAAGAA GCGGGTGCGA AAGGGTTTCT GGAAACAGCC TCCCCTCATC
661 TGGGATGTCA ATCCTAAACA GATTCGGATT CTCAACCCCT TCTTCATGGA GATTGCAGCT
721 GACAAACTGC TGAGCCTGCC AATGCAACAG CCACGGAAGA TTAAGCAGAA GCCCACCACG
781 GGCCTGTTGG CCATCACGCT GGCCCTCCAC CTCTGTGACT TGGTGCACAT TGCCGGCTTT
841 GGCTACCCAG ACGCCTACAA CAAGAAGCAG ACCATTCACT ACTATGAGCA GATCACGCTC
901 AAGTCCATGG CGGGGTCAGG CCATAATGTC TCCCAAGAGG CCCTGGCCAT TAAGCGGATG
961 CTGGAGATGG GAGC A CAA GAACCTCACG TCCTTCTGA
Protein Sequence of ST3GAL4 (SEQ ID NO: 7)
1 MCPAGWKLLA MLALVLWMV WYSISREDRY IELFYFPIPE KKEPCLQGEA ESKASKLFGN
61 YSRDQPIFLR LEDYFWVKTP SAYELPYGTK GSEDLLLRVL AITSSSIPKN IQSLRCRRCV
121 WGNGHRLRN SSLGDAINKY DWIRLNNAP VAGYEGDVGS KTTMRLFYPE SAHFDPKVEN
181 NPDTLLVLVA FKAMDFHWIE TILSDKKRVR KGFWKQPPLI WDVNPKQIRI LNPFFMEIAA
241 DKLLSLPMQQ PRKIKQKPTT GLLAITLALH LCDLVHIAGF GYPDAYNKKQ TIHYYEQITL
301 KSMAGSGHNV SQEALAIKRM LEMGAIKNLT SF
SEQ ID NO: 4
Beta-galactosamide alpha-2,6-sialyltransferase 1 Accession number NM 003032 Nucleotide sequence of ST6GAL1
1 ATGATTCACA CCAACCTGAA GAAAAAGTTC AGCTGCTGCG TCCTGGTCTT TCTTCTGTTT
61 GCAGTCATCT GTGTGTGGAA GGAAAAGAAG AAAGGGAGTT ACTATGATTC CTTTAAATTG
121 CAAACCAAGG AATTCCAGGT GTTAAAGAGT CTGGGGAAAT TGGCCATGGG GTCTGATTCC
181 CAGTCTGTAT CCTCAAGCAG CACCCAGGAC CCCCACAGGG GCCGCCAGAC CCTCGGCAGT
241 CTCAGAGGCC TAGCCAAGGC CAAACCAGAG GCCTCCTTCC AGGTGTGGAA CAAGGACAGC
301 TCTTCCAAAA ACCTTATCCC TAGGCTGCAA AAGATCTGGA AGAATTACCT AAGCATGAAC
361 AAGTACAAAG TGTCCTACAA GGGGCCAGGA CCAGGCATCA AGTTCAGTGC AGAGGCCCTG
421 CGCTGCCACC TCCGGGACCA TGTGAATGTA TCCATGGTAG AGGTCACAGA TTTTCCCTTC
481 AATACCTCTG AATGGGAGGG TTATCTGCCC AAGGAGAGCA TTAGGACCAA GGCTGGGCCT
541 TGGGGCAGGT GTGCTGTTGT GTCGTCAGCG GGATCTCTGA AGTCCTCCCA ACTAGGCAGA
601 GAAATCGATG ATCATGACGC AGTCCTGAGG TTTAATGGGG CACCCACAGC CAACTTCCAA
661 CAAGATGTGG GCACAAAAAC TACCATTCGC CTGATGAACT CTCAGTTGGT TACCACAGAG
721 AAGCGCTTCC TCAAAGACAG TTTGTACAAT GAAGGAATCC TAATTGTATG GGACCCATCT
781 GTATACCACT CAGATATCCC AAAGTGGTAC CAGAATCCGG ATTATAATTT CTTTAACAAC
841 TACAAGACTT ATCGTAAGCT GCACCCCAAT CAGCCCTTTT ACATCCTCAA GCCCCAGATG
901 CCTTGGGAGC TATGGGACAT TCTTCAAGAA ATCTCCCCAG AAGAGATTCA GCCAAACCCC
961 CCATCCTCTG GGATGCTTGG TATCATCATC ATGATGACGC TGTGTGACCA GGTGGATATT
1021 TATGAGTTCC TCCCATCCAA GCGCAAGACT GACGTGTGCT ACTACTACCA GAAGTTCTTC
1081 GATAGTGCCT GCACGATGGG TGCCTACCAC CCGCTGCTCT ATGAGAAGAA TTTGGTGAAG
1141 CATCTCAACC AGGGCACAGA TGAGGACATC TACCTGCTTG GAAAAGCCAC ACTGCCTGGC
1201 TTCCGGACCA TTCACTGCTA A
Op- Protein Sequence of ST6GAL1 (SEQ ID NO: 8)
1 MIHTNLKKKF SCCVLVFLLF AVICVWKEKK KGSYYDSFKL QTKEFQVLKS LGKLAMGSDS
61 QSVSSSSTQD PHRGRQTLGS LRGLAKAKPE ASFQVWNKDS SSKNLIPRLQ KIWKNYLSMN
121 KYKVSYKGPG PGIKFSAEAL RCHLRDHVNV SMVEVTDFPF NTSEWEGYLP KESIRTKAGP
181 WGRCAWSSA GSLKSSQLGR EIDDHDAVLR FNGAPTANFQ QDVGTKTTIR LMNSQLVTTE
241 KRFLKDSLYN EGILIVWDPS VYHSDIPKWY QNPDYNFFNN YKTYRKLHPN QPFYILKPQM
301 PWELWDILQE ISPEEIQPNP PSSGMLGIII MMTLCDQVDI YEFLPSKRKT DVCYYYQKFF
361 DSACTMGAYH PLLYEKNLVK HLNQGTDEDI YLLGKATLPG FRTIHC

Claims

1. A product comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient (e.g. a patient having serum AMH level of 0.05 pmol/L or above), wherein the product comprises a dose of, or a dose equivalent to, 2-24 μg, for example 2 to 15 μg, human derived recombinant FSH.
2. A product for use according to claim 1 comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH level of <15 pmol/L, wherein the product comprises a dose of, or dose equivalent to, 9 to 14 μg human derived recombinant FSH.
3. A product for use according to claim 2 wherein the product comprises a dose of, or dose equivalent to 12 μg human derived recombinant FSH.
4. A product for use according to claim 2 or claim 3 wherein the treatment of infertility comprises a step of determining the serum AMH level of the patient, and a step of
administering the dose to a patient having serum AMH level of <15 pmol/L. 5. A product for use according to claim 1 comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH level of≥15 pmol/L, wherein the product comprises a dose of, or dose equivalent to, 5 to 12.
5 μg human derived recombinant FSH.
6. A product for use according to claim 5 wherein the treatment of infertility comprises a step of determining the serum AMH level of the patient, and a step of administering the dose to a patient having serum AMH level of≥15 pmol/L.
7. A product for use according to claim 1 comprising follicle stimulating hormone (FSH) for use in the treatment of infertility in a patient having serum AMH level of≥15 pmol/L, wherein the product is for administration at a dose of, or dose equivalent to, 0.09 to 0.19 μg human derived recombinant FSH per kg bodyweight of the patient.
8. A product for use according to claim 7 in the treatment of a patient having serum AMH level of 15 to 24.9 pmol/L, wherein the product is for administration at a dose of, or dose equivalent to, 0.14 to 0.19 μg human derived recombinant FSH per kg bodyweight of the patient. 9. A product for use according to claim 7 in the treatment of a patient having serum AMH level of 25 to 34.
9 pmol/L, wherein the product is for administration at a dose of, or dose equivalent to, 0.1 1 to 0.14 human derived recombinant FSH per kg bodyweight of the patient.
10. A product for use according to claim 7 in the treatment of a patient having serum AMH level of≥ 35 pmol/L, wherein the product is for administration at a dose of, or dose equivalent to, 0.10 to 0.1 1 μg human derived recombinant FSH per kg bodyweight of the patient.
1 1. A product for use according to any of claims 7 to 10 wherein the treatment of infertility includes a step of determining the serum AMH level of the patient, and a step of administering the dose to a patient having the defined serum AMH level.
12. A product according to any preceding claim wherein the follicle stimulating hormone (FSH) is a human derived recombinant FSH.
13. A product according to any preceding claim wherein the FSH includes a2, 3- and a2, 6- sialylation.
14. A product according to claim 13 wherein 25 to 50%, for example 30 to 50%, of the total sialylation is a2, 6- sialyation.
15. A product according to claim 13 or claim 14 wherein 50 to 70% of the total sialylation is a 2,3- sialyation.
16. A product according to any preceding claim wherein the FSH includes mono-, di-, tri- and tetra-sialylated glycan structures, wherein 15-24%, for example 17-23% of the sialylated glycan structures are tetrasialylated glycan structures.
17. A product comprising follicle stimulating hormone (FSH) for use in the treatment of infertility according to any preceding claim, wherein the product further comprises a salt comprising a pharmaceutically acceptable alkali metal cation selected from the group consisting of Na+- or K+- salts, or a combination thereof.
18. A method of treatment of infertility comprising:
(a) measuring the serum AMH level of a subject; and
(b) administration to the subject a dose of, or a dose equivalent to, 2-24 μg, for example 2 to 15 μg, human derived recombinant FSH.
19. A method of treatment of infertility comprising: (a) determining the serum AMH level of a subject; and (b) administering a dose of, or dose equivalent to, 9 to 14 μg human derived recombinant follicle stimulating hormone (FSH) to a subject having serum AMH level of <15 pmol/L.
20. A method of treatment of infertility comprising: (a) determining the serum AMH level of a subject; and (b) administering a dose of, or dose equivalent to, 5 to 12.5 μg human derived recombinant follicle stimulating hormone (FSH) to a (the) subject having serum AMH level of >15 pmol/L.
21. A method of treatment of infertility comprising: (a) determining the serum AMH level of a subject; and (b) administering a dose of, or dose equivalent to, 0.09 to 0.19 μg human derived recombinant FSH per kg bodyweight of the subject, wherein the subject has serum AMH level of >15 pmol/L.
22. A method according to claim 21 comprising a step of administering a dose of, or dose equivalent to, 0.14 to 0.19 μg human derived recombinant FSH per kg bodyweight of the subject, the subject having serum AMH level of 15 to 24.9 pmol/L.
23. A method according to claim 21 comprising a step of administering a dose of, or dose equivalent to, 0.1 1 to 0.14 μg human derived recombinant FSH per kg bodyweight of the subject, the subject having serum AMH level of 25 to 34.9 pmol/L.
24. A method according to claim 21 comprising a step of administering a dose of, or dose equivalent to, 0.10 to 0.1 1 μg human derived recombinant FSH per kg bodyweight of the subject, the subject having serum AMH level of≥ 35 pmol/L.
25. A method of treatment of infertility comprising: (a) determining the serum AMH level of a subject; and (b) administering a dose of, or dose equivalent to, 0.15 to 0.17 μg human derived recombinant FSH per kg bodyweight of the subject, wherein the subject has serum AMH level of <15 pmol/L.
26. A product comprising follicle stimulating hormone (FSH) for use in the manufacture of a medicament for use in the treatment of infertility in a patient (e.g. a patient having serum AMH level of 0.05 pmol/L or above), wherein the product comprises a dose of, or a dose equivalent to, 2-24 μg, for example 2 to 15 μg, human derived recombinant FSH.
PCT/EP2012/065507 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation WO2013020996A1 (en)

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JP2014524372A JP6161609B2 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
IL300380A IL300380A (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
KR1020197036505A KR20190140098A (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
DK12753675.3T DK2741763T3 (en) 2011-08-08 2012-08-08 COMPOSITION FOR CONTROLLED OVARI STIMULATION.
SI201230438T SI2741763T1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
RS20160080A RS54561B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
PL19213649T PL3646881T3 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
BR122020001644-2A BR122020001644B1 (en) 2011-08-08 2012-08-08 COMPOSITION COMPRISING RECOMBINANT FOLLICLE STIMULATING HORMONE AND USE THEREOF FOR THE TREATMENT OF INFERTILITY
US14/237,697 US9694052B2 (en) 2011-08-08 2012-08-08 FSH composition and methods for controlled ovarian stimulation
CN201280038831.7A CN103732243A (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
EP21207717.6A EP4005588B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
KR1020207029774A KR20200121918A (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
AU2012293647A AU2012293647B2 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
PL14184409T PL2821080T3 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
EP12753675.3A EP2741763B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
EP19164277.6A EP3566712B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
EP19213649.7A EP3646881B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
BR112014002884A BR112014002884A2 (en) 2011-08-08 2012-08-08 composition for controlled ovarian stimulation
KR1020147005938A KR102014469B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
PL19164277T PL3566712T3 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
EP14184409.2A EP2821080B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
KR1020217037512A KR102499969B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
PL18167552T PL3395357T3 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
EP18167552.1A EP3395357B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
KR1020217023662A KR102353144B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
RU2014102924A RU2613324C3 (en) 2011-08-08 2012-08-08 COMPOSITION FOR CONTROLLED OVARIAN STIMULATION
EP20186935.1A EP3756681B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
PL17154031T PL3195875T3 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
NZ620369A NZ620369B2 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
EP17154031.3A EP3195875B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
PL12753675T PL2741763T3 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
KR1020237004855A KR20230024442A (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
KR1020197024335A KR102111514B1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
CA2844282A CA2844282A1 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
EP23208228.9A EP4306174A3 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
MX2014001489A MX348981B (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation.
ES12753675.3T ES2562648T3 (en) 2011-08-08 2012-08-08 Composition for controlled ovarian stimulation
IL230696A IL230696B2 (en) 2011-08-08 2014-01-28 Composition for controlled ovarian stimulation
ZA2014/00952A ZA201400952B (en) 2011-08-08 2014-02-07 Composition for controlled ovarian stimulation
HK14111597.8A HK1198005A1 (en) 2011-08-08 2014-11-17 Composition for controlled ovarian stimulation
HRP20160145TT HRP20160145T1 (en) 2011-08-08 2016-02-09 Composition for controlled ovarian stimulation
SM201600038T SMT201600038B (en) 2011-08-08 2016-02-10 COMPOSITION FOR A CONTROLLED OVARIAN STIMULATION
AU2016273925A AU2016273925B2 (en) 2011-08-08 2016-12-15 Composition for controlled ovarian stimulation
US15/637,962 US10624953B2 (en) 2011-08-08 2017-06-29 Human-derived recombinant FSH for controlled ovarian stimulation
AU2018203222A AU2018203222B2 (en) 2011-08-08 2018-05-09 Composition for controlled ovarian stimulation
US16/851,260 US11291708B2 (en) 2011-08-08 2020-04-17 Human-derived recombinant FSH for controlled ovarian stimulation
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