WO2012144395A1

WO2012144395A1 - Photoacoustic imaging apparatus and method therefor

Info

Publication number: WO2012144395A1
Application number: PCT/JP2012/059950
Authority: WO
Inventors: Kazuhiko Fukutani
Original assignee: Canon Kabushiki Kaisha
Priority date: 2011-04-18
Filing date: 2012-04-05
Publication date: 2012-10-26
Also published as: JP2012223283A; US9554709B2; CN103492871B; US20140046165A1; EP2699896A1; CN103492871A; US10206586B2; US20170100039A1; JP5783779B2

Abstract

There is provided a specimen information acquisition apparatus for performing photoacoustic imaging and reconstruction of absorption coefficient maps that can obtain an image in which an influence of a local fluence distribution in a specimen is reduced. A signal processing apparatus includes a region extraction unit and an optical constant estimation unit. The region extraction unit extracts two or more regions that have a known absorption coefficient, from an optical characteristic value distribution of the specimen. The optical constant estimation unit estimates an optical constant of a background of the specimen by using optical characteristic values of the two or more regions extracted by the region extraction unit and a local fluence distribution obtained from an optical constant which is set in advance for the specimen background.

Description

DESCRIPTION

PHOTOACOUSTIC IMAGING APPARATUS AND METHOD THEREFOR

Technical Field

[0001] The present invention relates to a specimen information acquisition apparatus which generates an optical characteristic value distribution of a specimen by using photoacoustic waves, and a method used for the specimen information acquisition apparatus to acquire specimen information .

Background Art

[0002] Research on optical imaging apparatuses which emit light from a light source such as a laser into a living body and which create images that represent information about the inside of the living body which is obtained on the basis of the incident light has been actively carried out in the medical field. One technique in this optical imaging technology is photoacoustic imaging (PAI) . In PAI, a living body is irradiated with pulsed light produced from a light source, acoustic waves (typically, ultrasonic waves) are detected which are produced from the tissue of the living body that absorbs the energy of the pulsed light which propagates and diffuses in the living body, and imaging (creation of an image) is performed on the basis of the detected signals so that the created image represents live body information.

[0003] That is, by utilizing a difference between the light energy absorption ratio of a specimen area such as a tumor and that of other tissue of the specimen, elastic waves which are produced when the specimen area absorbs received light energy and momentarily expands are detected by an acoustic wave probe (also referred to as a probe or a transducer) which is a detector. The detected signals are analyzed so that an image that represents an optical

characteristic value distribution, such as an initial

pressure distribution, an optical energy absorption density distribution, or an optical absorption coefficient

distribution, is obtained.

[0004] These pieces of image information can be utilized for quantitative measurement of a specific substance in a specimen, such as hemoglobin concentration included in blood, or oxygen saturation of blood, by measuring the specimen by means of light having various wavelengths.

[0005] Recently, by using this photoacoustic imaging, preclinical research on imaging of a vessel image of a small animal, and clinical research on application of this

principle to diagnosis of, for example, breast cancer, prostatic cancer, and carotid plaque have been actively carried out.

[0006] In photoacoustic imaging, an initial sound pressure (Po) for photoacoustic waves produced from an absorber due to light absorption may be expressed by the following

equation .

[0007]

[Math. 1]

0 = Γ · μ_α · Φ - (1)

[0008] Here, Γ represents a Grueneisen coefficient, which is obtained by dividing the product of a thermal expansion coefficient (β) and the square of the speed of sound (c) by a specific heat at constant pressure (C_P) . The symbol μ_β represents the optical absorption coefficient of an absorber, and Φ represents a quantity of light (local optical fluence) with which the absorber is irradiated. The Grueneisen

coefficient Γ is known to have a substantially constant value for the same tissue. Accordingly, by measuring and analyzing changes in a sound pressure P, which is the

magnitude of an acoustic wave, in multiple positions, the product of μ₃ and Φ, that is, an optical energy absorption density distribution can be obtained.

[0009] In photoacoustic imaging, as understandable from Equation (1) described above, to determine an absorption coefficient (μ₃) distribution in a specimen, which is

required for quantitative imaging, from the measurement result of a sound pressure (P), it is necessary to determine a quantity of light (Φ) , with which an absorber that

produces photoacoustic waves is irradiated, in some way.

However, light introduced into the inside of a specimen

(especially, a living body) exhibits behavior such as strong diffusion, so that it is difficult to estimate a quantity of light with which an absorber is irradiated.

[0010] Therefore, when no specific measures are taken, photoacoustic imaging has a problem in that only an image that represents an optical energy absorption density (μ₃ x Φ) distribution or an initial sound pressure (P₀)

distribution obtained by multiplying the optical energy absorption density distribution by Γ can be generated on the basis of the measurement results of sound pressures of acoustic waves. For example, even when light absorbers are composed of the same substance and have the same absorption coefficient, such light absorbers are displayed in different contrasts in accordance with an influence of a local fluence distribution in the living body (for example, a distance from a light source) .

Citation List

Non Patent Literature

[0011] NPL 1 "Photoacoustic imaging in biomedicine" , M. Xu, L. V. Wang, REVIEW OF SCIENTIFIC INSTURUMENT, 77, 041101, 2006 Summary of Invention

Technical Problem

[0012] As described above, photoacoustic imaging has a problem in that it is difficult to precisely compute a local fluence distribution (Φ) with which an absorber is

irradiated, and, as a result, it is difficult to compute an optical characteristic value distribution such as an

absorption coefficient distribution properly.

[0013] Therefore, the present invention provides a specimen information acquisition apparatus which can obtain an optical characteristic value distribution in which an influence of a local fluence distribution (Φ) with which an absorber is irradiated is reduced, and a method for

acquiring specimen information.

Solution to Problem

[0014] To overcome the above-described problem, there is provided a specimen information acquisition apparatus including a light source, a detector, and a signal

processing apparatus. The light source irradiates a

specimen with light. The detector detects an acoustic wave that is produced by the specimen due to the light. The signal processing apparatus generates an optical

characteristic value distribution of the specimen on the basis of a detected signal that is obtained from the

detector. The signal processing apparatus includes a region extraction unit and an optical constant estimation unit. The region extraction unit extracts two or more regions that have a known absorption coefficient, from the optical characteristic value distribution of the specimen. The optical constant estimation unit estimates an optical constant of a background of the specimen by using optical characteristic values of the two or more regions extracted by the region extraction unit and a local fluence

distribution obtained from an optical constant that is set in advance for the specimen background.

Advantageous Effects of Invention

[0015] The present invention can provide a specimen information acquisition apparatus which can obtain an optical characteristic value distribution in which an influence of a local fluence distribution (Φ) with which an absorber is irradiated is reduced, and a method for

acquiring specimen information.

Brief Description of Drawings

[0016] Fig. 1 is a schematic diagram illustrating a configuration of a specimen information acquisition

apparatus according to an embodiment of the present

invention.

[0017] Fig. 2 is a flowchart of an exemplary process on detected signals according to an embodiment of the present invention . [0018] Fig. 3A is a diagram illustrating an exemplary image, and Figs. 3B to 3D are graphs showing calculation results .

[0019] Fig. 4 is a diagram illustrating an exemplary image in which an influence of a local fluence distribution is reduced.

[0020] Fig. 5 is a schematic diagram illustrating an apparatus configuration which is described in a second embodiment .

[0021] Figs. 6A to 6C are diagrams for explaining examples in which two or more regions are extracted from an optical characteristic value distribution.

Description of Embodiments

[0022] The present invention will be described in more detail below referring to the drawings. In principle, the same components are designated with the same reference numeral, and will not be described.

[0023] Referring to Fig. 1, a configuration of a specimen information acquisition apparatus according to an embodiment of the present invention will be described. The specimen information acquisition apparatus according to the

embodiment is an apparatus which creates an image that represents optical characteristic value information about the inside of a specimen.

[0024] Herein, the optical characteristic value information typically indicates an initial sound pressure distribution (P₀) or an optical energy absorption density distribution (μ₃ ·Φ) , or an optical absorption coefficient distribution (μ₃) which is derived from these.

Basic Con iguration

[0025] The specimen information acquisition apparatus according to the embodiment has a basic hardware

configuration including a light source 11, a detector 17, and a signal processing apparatus 19. Pulsed light 12 emitted from the light source 11 is guided while being processed by an optical system including, for example, a lens, a mirror, an optical fiber, and a diffuser plate so as to be shaped in a desired form, and enters a specimen 15 such as a living body.

[0026] When part of optical energy that propagates through the inside of the specimen 15 is absorbed into light

absorbers 14 such as vessels, acoustic waves 16 which are elastic waves are produced due to thermal expansion of the light absorbers 14. These may be called "photoacoustic waves" .

[0027] The acoustic waves 16 are detected by the detector 17, and are amplified and converted into digital signals by a signal collector 18. Then, an optical characteristic value distribution for the specimen 15 is generated by the signal processing apparatus 19, and is output as image data. This image data is displayed as an image on a display apparatus 20.

Method for Acquiring Specimen Information

[0028] An exemplary method for acquiring specimen

information which is performed by the signal processing apparatus 19 and which is a characteristic of the present invention will be described referring to the drawings. The reference numerals in the description each correspond to the number of a corresponding one of the processes in Fig. 2.

[0029] In step S201, an optical characteristic value distribution is generated. In this step, image

reconstruction is performed using received signals so that the optical characteristic value distribution (first optical characteristic value distribution) for the specimen is generated. Here, an optical characteristic value

distribution for a specimen means an initial sound pressure distribution or an optical energy absorption density

distribution which is obtained by multiplying an absorption coefficient distribution by a local fluence distribution, which are expressed by, for example, Equation (1) . This first optical characteristic value distribution is dependent on the local fluence distribution.

[0030] For example, a first generation unit 19a generates the first optical characteristic value distribution by using received digital signals which are detected signals and which are obtained from the signal collector 18.

[0031] This process can be performed by using various image reconstruction processes used in typical photoacoustic imaging. For example, a time-domain back-projection method and a Fourier transform method, which are typically used in tomography technology, may be used as an image

reconstruction algorithm. When it is possible to take a large amount of time for the reconstruction, an image reconstruction technique such as a model-based method that performs a large number of matrix operations may be used. Typical image reconstruction techniques for PAI include a Fourier transform method, a back-projection method, a time reversal method, a deconvolution method, and a model-based method. In the present invention, any image reconstruction technique may be used.

[0032] When, for example, a focus-type detector that performs detection for limited observation points is used and a received signal from such a detector is originally proportional to an image in the depth direction, the image reconstruction need not be performed and the received signal may be directly converted into image data.

[0033] In step S202, two or more regions from the optical characteristic value distribution is extracted. In this step, two or more regions that have known absorption

coefficients are extracted from the first optical characteristic value distribution which is generated in step S201. As long as the absorption coefficients of the two or more regions are known, the absorption coefficients of the regions may be the same or may be different. In addition, as long as the regions to be extracted have any quantitative data, the regions are not necessarily image data.

[0034] Fig. 6A illustrates an exemplary method for extracting regions. Since light 60 with which a specimen 61 is irradiated is attenuated in accordance with the incident depth, light quantities during the irradiation are different in regions 63 and 64 in a vessel 62. Since the regions 63 and 64 are portions of the same vessel, the absorption coefficients of these regions are the same. In this step, two or more regions may be extracted from the same light absorber as described above.

[0035] Fig. 6B illustrates an example in which two or more regions are extracted from different light absorbers. Thus, in this step, as long as the absorption coefficients of regions 65 and 66 are known, these regions may be extracted. For example, a contrast agent that has an absorption

coefficient that is known in advance may be introduced and utilized .

[0036] Fig. 6C illustrates an example in which two or more regions are extracted from a light absorber provided on the outside of the specimen 61. Thus, in this step, a light absorber 67 may be provided on the outside of the specimen 61, and regions 68 and 69 which are portions of the light absorber 67 may be extracted.

[0037] The apparatus may automatically extract regions, or an operator using the apparatus may specify regions, so that the regions are extracted.

[0038] For example, a region extraction unit 19b extracts, for example, image data regions that have the same

absorption coefficient from an image that represents the first optical characteristic value distribution obtained by the first generation unit 19a. That is, positions in which the absorption coefficients are the same in at least one light absorber are specified from image data of the at least one light absorber.

[0039] Fig. 3A illustrates an example of the first optical characteristic value distribution obtained through the process performed in step S201. In Fig. 3A, the regions denoted by reference characters A, B, and C represent light absorbers which have been subjected to imaging. The

contrast (color intensity) of each region represents, for example, the magnitude of the initial sound pressure or the optical energy absorption density. Although the absorption coefficients of the light absorbers corresponding to the reference characters A, B, and C in Fig. 3A are the same, the contrasts are different. [0040] In step S203, optical constants of a specimen background is estimated. In this step, the optical

constants of the specimen background are estimated by using optical characteristic values, such as initial sound

pressures or optical energy absorption densities, of the two or more regions extracted in step S202, and optical

constants which are set in advance for the specimen

background .

[0041] The optical constants of the specimen background that are to be estimated in this step include, for example, an absorption coefficient {μ_Ά ^ύ) , a scattering coefficient

( μ₃ ¹³ ) , and an anisotropic parameter (g^b) of the specimen background. A scattering coefficient {μ^⁰) and an

anisotropic parameter (g^b) are included in a reduced

scattering coefficient ^_s ^'b) , and an absorption coefficient

{μ^) and a reduced scattering coefficient ^_s ^'b) may be obtained as the optical constants of the specimen background. Furthermore, an absorption coefficient ( μ₃ ¹³ ) and a reduced scattering coefficient ^_s ^'b) are included in an effective attenuation coefficient ^^b _{ef f} ) , and an effective attenuation coefficient (

) may be obtained as the optical constant of the specimen background.

[ 0042 ] For example, an optical constant estimation unit 19c estimates the optical constants of the specimen

background from pieces of extracted image data that have the same absorption coefficient. In the following description, an absorption coefficient and a reduced scattering

coefficient are estimated as an example of the optical constants of the specimen background.

[0043] In the case where the specimen is a living body, when the emitted light is near-infrared light of 500 to 900 nm, the main light absorber in the living body is

oxygenated/deoxygenated hemoglobin. In other words, an image of vessels having a large amount of hemoglobin is mainly created. Here, it is assumed that the absorption coefficients of blood in vessels located in any positions be the same.

[0044] Under this assumption, the values of an absorption coefficient (μ₃) and a Grueneisen coefficient (Γ) in

Equation (1) are constant in a vessel image. That is, Γ and μ₃ are constants, so that Po(r) is a function that is dependent only on a local fluence distribution O(r). The local fluence distribution X>(r) may be obtained through calculation when the optical constants, the shape, the light irradiation distribution (light source distribution) of the specimen are determined.

[0045] Specific description will be made using equations. It is assumed that the initial sound pressure distribution P₀(r) is obtained as the first optical characteristic value distribution. In addition, it is assumed that the quantity of light at a position r of a light absorber is determined by the absorption coefficient ( μ₃ ^ΐ3 ) and the reduced

scattering coefficient ^_s ^'b) of the specimen background.

Equation (1) will be the following equation.

[0046]

[Math. 2]

¾('^■) = Γ(Γ) · μ_β (Γ)·Φ(Γ, _μ; , μ·ί) -(2)

[0047 ] Here, r represents a position of the light absorber. It is assumed that all of the light absorbers are vessels, and that the absorption coefficients ( μ₃) of the vessels are constant regardless of the oxygen saturation. In this case, Γ and μ₃ are constants. Consequently, Po(r) is a function that is dependent only on the local fluence distribution (Φ) .

[0048]

[Math. 3]

P₀(r) * A - (r ^b _a,tf ) -(3)

[0049] Here, A is a proportional constant, and is obtained by multiplying the Γ by μ₃, which are constants. When the specimen background is a uniform medium and diffusion

approximation for light is established, the quantity of light O(r) at a position r of the light absorber may be obtained using, for example, the following diffusion

equation .

[0050]

[Math. 4] · Φ(Γ) - £>V²<D(r) = S(r) · · · (4)

[0051] Here, D is a diffusion constant, and is expressed by the equation D = 1/3 (μ₃ ¹³ + ^_a'^h) , and S(r) represents a light irradiation distribution. In other words, when

optical constants of the specimen background, such as a diffusion constant D and an absorption coefficient μ^, boundary conditions such as the shape of the specimen, and a light irradiation distribution S (r) are determined, the local fluence distribution O(r) of the specimen background may be obtained through forward model calculation

(simulation) . Here, it is assumed that the absorption

coefficient (μ₃ ¹³) and the reduced scattering coefficient

^_s ^'b) of the specimen background be variables, and the local fluence distribution obtained through calculation is defined as O_sim(r, μ₃\ μ₅'^ύ) .

[0052] Then, an objective function E is defined as follows.

[0053]

[Math. 5]

E =∑^[p₀(r) - A ^_sim(r^ _s ^b )f - . (5)

r

[0054] By minimizing the objective function E, an

absorption coefficient (μ₃ ^ι>) and a reduced scattering

coefficient ^_s ^'b) , which are the optical constants of the specimen background, may be estimated. Specifically, a typical optimization technique is used, and while the absorption coefficient and the reduced scattering coefficient are repeatedly updated and <J>_Si_m is obtained through simulation, E may be minimized. For example, the absorption coefficient (μ₃ ¹³) and the reduced scattering coefficient ^_s' ) , which are the optical constants of the specimen background, are set as appropriate, and a local fluence distribution 0_Si_m(r, μ₃ ¹ ^_s'^b) of the specimen is calculated through simulation.

[0055] The average absorption coefficient (μ₃ ¹⁵) and the average reduced scattering coefficient ^_s ^'b) of the specimen background are repeatedly changed so as to cause the

minimization of a sum of the differences between the result obtained by multiplying the value <I>_Si_m(r, ,

by the predetermined proportional constant A and each of the pieces of image data that have the same absorption coefficient and that are obtained in step S202.

[0056] For example, Fig. 3B illustrates the result obtained through the above-described minimization. The vertical axis represents a log value of the objective function E, and the horizontal axis represents the number of repetitions of the calculation.

[0057] Fig. 3C illustrates the relationship between an absorption coefficient of the specimen background and the number of repetitions of the calculation. Fig. 3D

illustrates the relationship between a reduced scattering coefficient of the. specimen background and the number of repetitions of the calculation. From these graphs, it is found that the value of the objective function E becomes small through the optimization computation (Fig. 3B) , and that the absorption coefficient and the reduced scattering coefficient converge on certain values as the value of the objective function E becomes small (Figs. 3C and 3D). The final convergence values are defined as average optical constants of the specimen background.

[0058] As described above, by using such a repeated

computation method, the optical constants of the specimen background may be estimated from an optical characteristic value distribution, such as an initial sound pressure

distribution or an optical energy absorption density

distribution.

[0059] When the light absorber is a vessel, the absorption coefficient of the vessel varies in accordance with oxygen saturation. Accordingly, a certain wavelength that causes the absorption coefficient not to vary in accordance with an oxygen saturation value, for example, a wavelength of 797 nm, is desirably selected.

[0060] In the above-described embodiment, certain examples, such as a method for estimating a local fluence distribution, an objective function, and an optimization technique for the objective function are employed. However, any methods and techniques may be used as the method for estimating a local fluence distribution, a method for selecting an objective function, and the optimization technique for the objective function as long as the optical constants of the specimen background are eventually determined by using these.

[0061] By performing the step described above, optical constants of the specimen background may be estimated from pieces of image data that have the same absorption

coefficient .

[0062] In step S204, an optical characteristic value distribution by using the estimated optical constant is generated. In this step, a local fluence distribution is computed using the optical constants of the specimen background that are estimated in step S203, and the optical characteristic value distribution (second optical

characteristic value distribution) of the specimen is generated by using the values of this local fluence

distribution. The second optical characteristic value distribution is, for example, an optical absorption

coefficient distribution in which an influence of the local fluence distribution is reduced.

[0063] For example, a second generation unit 19d computes a local fluence distribution in the specimen by using the optical constants of the specimen background obtained in step S203 and, for example, the diffusion equation expressed by Equation (4). Then, by using the obtained local fluence distribution, the first optical characteristic value

distribution obtained in step S201 is corrected. Fig. 4 illustrates an optical characteristic value distribution obtained by dividing the first optical characteristic value distribution in Fig. 3A by the local fluence distribution computed using the absorption coefficient and the reduced scattering coefficient of the specimen background that are obtained from the results in Figs. 3C and 3D. In Fig. 3A, the light absorbers A, B, and C that have the same

absorption coefficient are represented by images having different contrasts due to the influence of the local

fluence distribution. In contrast, in Fig. 4, the light absorbers are represented by images having the same contrast. That is, the second optical characteristic value

distribution in which the influence of the local fluence distribution is reduced is obtained.

[0064] As described above, an example in which a local fluence distribution is obtained using a diffusion equation is described. However, any method, such as a method in which a radiative transport equation is precisely solved, or the Monte Carlo method, may be employed as long as a local fluence distribution is obtained.

[0065] As described above, the second optical

characteristic value distribution is obtained by dividing the first optical characteristic value distribution by the local fluence distribution. However, other computation methods may be used to obtain the second optical

characteristic value distribution.

[0066] By performing the above steps, even when the optical constants for the specimen are not known, a specimen information acquisition apparatus can be provided which can obtain an image in which an influence of the local fluence distribution in a specimen is reduced.

[0067] The first generation unit 19a, the region

extraction unit 19b, the optical constant estimation unit 19c, and the second generation unit 19d are functional blocks, which are typically performed by software. The execution of the software may be performed in the same hardware, or may be performed in different pieces of

hardware .

[0068] Now, an exemplary specific configuration according to the embodiment will be described.

Light Source 11

[0069] When the specimen is a living body, the light source 11 emits light having a certain wavelength which is absorbed by a specific one of the components of the living body. The light source may be provided integrally with the specimen information acquisition apparatus according to the embodiment, or may be provided separately from the specimen information acquisition apparatus. The light source is desirably a pulsed light source that can generate pulsed light on the order of several to hundreds nano-seconds .

Specifically, to generate photoacoustic waves efficiently, the pulse width of about 10 nano-seconds is used. A laser is desirably used as the light source because it produces a large amount of output. Instead of a laser, for example, a light-emitting diode may be used. Various lasers, such as a solid laser, a gas laser, a dye laser, and a semiconductor laser, may be used. A light source controller (not

illustrated) controls, for example, the timing of the irradiation and optical intensity. This light source controller is typically provided integrally with the light source. Usable wavelengths of the light source are

desirably wavelengths that cause light to propagate into the inside of the specimen. Specifically, when the specimen is a living body, the wavelength is equal to or more than 500 nm and equal to or less than 1200 nm.

Light Absorber 14 and Specimen 15

[0070] The light absorber 14 and the specimen 15, which are not included in the specimen information acquisition apparatus according to the embodiment of the present

invention, will be described below. The specimen

information acquisition apparatus according to the

embodiment of the present invention which uses photoacoustic waves aims to be mainly used for angiography, diagnosis for malignant tumors, vascular diseases, or the like for a human being or an animal, observation after chemotherapy, or the like. Accordingly, it is presumed that the specimen 15 is a living body, particularly, a part to be checked for

diagnosis, such as a breast, a finger, or a limb of a human being or an animal. When a small animal such as a mouse is checked, the whole small animal may be checked in addition to a specific part. Components that have a relatively high absorption coefficient in the specimen are used as the light absorber 14 in the specimen. Such components, depending on a usable light wavelength, include, for example, oxygenated or deoxygenated hemoglobin, a vessel which contains a large amount of hemoglobin, and a malignant tumor which contains many neovascular vessels, when it is assumed that a human body is to be checked.

Detector 17

[0071] The detector 17, which detects photoacoustic waves that are produced, for example, from the specimen surface and in the specimen by means of pulsed light, detects acoustic waves and converts the acoustic waves into electric signals which are analog signals. This detector may be a probe or a transducer. Any detector, such as a transducer using piezoelectric phenomena, a transducer using optical resonance, or a transducer using changes in capacitance, may be used as long as the detector detects an acoustic wave signal. It is desirable that the detector 17 according to the embodiment be typically a detector in which sensing elements are arranged in one or two dimensions. Such multidimensional array elements are used so that acoustic waves can be simultaneously detected in multiple positions, thereby reducing the measurement time. As a result, an influence of the vibration of the specimen or the like can be reduced. If scanning is performed using a single

detector having only one detector in such a manner that acoustic waves can be detected in multiple positions, a one or two dimensional array detector need not be used. As in Fig. 1, scanning is performed using the detector 17 in a region surrounding the specimen 15, or multiple detectors are prepared like an array-type detector, so that the acoustic waves 16 are desirably detected at various angles. Signal Collector 18

[0072] The specimen information acquisition apparatus according to the embodiment desirably has the signal collector 18 which amplifies an electric signal obtained from the detector 17 and converts the electric signal, which is an analog signal, into a digital signal. The signal collector 18 typically includes an amplifier, an A/D converter, and a field programmable gate array (FPGA) chip. When the signal collector 18 obtains multiple detected signals from the detector 17, it is desirable that the signal collector 18 can simultaneously process multiple signals. Accordingly, the time period before the formation of an image can be reduced. A term "detected signal" herein encompasses an analog signal obtained from the detector 17 and a digital signal which is a result of the A/D conversion using the analog signal.

Signal Processing Apparatus 19

[0073] The signal processing apparatus 19 performs an image reconstruction process and a computation process using the received digital signals obtained from the signal collector 18, and obtains an image in which an influence of the local fluence distribution in the specimen is reduced. In particular, the signal processing apparatus 19 according to the embodiment of the present invention performs the above-described computation process.

[0074] In general, a computer such as a workstation is typically used as the signal processing apparatus 19. The process is performed by software which is produced through programming in advance. The first generation unit 19a, the region extraction unit 19b, the optical constant estimation unit 19c, and the second generation unit 19d are typically provided as a computer such as a workstation and the

software attached to the computer.

Display Apparatus 20 [0075] The display apparatus 20 displays image data that is output from the signal processing apparatus 19, and a liquid crystal display or the like is typically used as the display apparatus 20. The display apparatus 20 may be provided separately from the specimen information

acquisition apparatus according to the embodiment of the present invention.

First Embodiment

[0076] An example for the specimen information acquisition apparatus using photoacoustic imaging to which the present invention is applied will be described. The description will be made using the schematic diagram illustrating the apparatus in Fig. 1.

[0077] In a first embodiment, a Q-switch YAG laser which produces pulsed light of 1064-nm wavelength and about 10 nano-seconds is used as the light source 11. The energy of the light pulse emitted from the pulsed laser light 12 is 0.6 J. An optical system is set in such a manner that the top surface of the specimen is irradiated with the pulsed light after the pulsed light is expanded so as to have a radius of about 20 mm by using a different optical system including a mirror and a beam expander.

[0078] A phantom which mimics a living body in terms of the optical constants is used as the specimen 15. The base of the phantom is made from polyurethane rubber, and the optical constants are adjusted by using titanium oxide and ink. The base phantom has an absorption coefficient of 0.0047 mm^-1 and a reduced scattering coefficient of 0.7 mm^"1.

[0079] Three columnar objects of 2-mm diameter are embedded in the base phantom as the light absorber 14. The absorption coefficients of the columnar objects are the same; the absorption coefficient is 0.08 mm^-1 and the

Grueneisen coefficient is 0.9.

[0080] This phantom is placed in a water tank, the

detector 17 is set so as to be operated around the phantom as illustrated in Fig. 1, and the top surface of the phantom is irradiated with the pulsed light 12. In the first embodiment, it is difficult to produce an arch-shaped array- type probe, so that a single-element-type probe is used as the detector 17.

[0081] Then, while light is emitted, scanning is performed with the detector 17, and the detector 17 receives

photoacoustic waves produced in a range of 360° around the phantom.

[0082] The received analog signal is converted into digital data for the photoacoustic signal by using an amplifier, an A/D converter, and an FPGA which are included in the signal collector 18. Then, the obtained digital data is transferred to a workstation (WS) which is the signal processing apparatus 19, and is stored in the WS . [0083] Then, the first generation unit 19a which is a software program in the WS performs image reconstruction, and computes an initial sound pressure distribution which is a photoacoustic image.

[0084] Then, the region extraction unit 19b which is a software program in the WS extracts regions that have a contrast higher than that of the surroundings from the obtained initial sound pressure distribution image, so that columnar light absorber images are extracted.

[0085] Then, the optical constant estimation unit 19c which is a software program in the WS minimizes the

objective function which is the same as Equation (5) . Here, the absorption coefficient and the Grueneisen coefficient of the absorber which are known in advance are used as the proportional constant A. In the minimization, the initial values of the absorption coefficient and the reduced

scattering coefficient of the specimen background are set to 0.007 mm^-1 and 0.4 mm^"1, respectively. The obtained results at that time are shown in Figs. 3B, 3C, and 3D.

[0086] In Fig. 3B, the values of the absorption

coefficient and the reduced scattering coefficient of the phantom background converge after about 33 repetitions, and the final values of the absorption coefficient and the reduced scattering coefficient are 0.0049 mm^"1 and 0.68 mm^"1, respectively. These values are approximately equal to the values of the base phantom. Therefore, this method enables detection of an absorption coefficient and a reduced

scattering coefficient which are average optical constants of the background of the phantom which is a specimen.

[0087] Furthermore, the second generation unit 19d which is a software program in the WS uses these values to compute the local fluence distribution in the phantom, and

normalizes the initial sound pressure distribution image by using the local fluence distribution. Thus, an image in which an influence of the local fluence distribution is reduced can be obtained.

Second Embodiment

[0088] An example for a specimen information acquisition apparatus using photoacoustic imaging to which the present invention is applied will be described. In the first embodiment, an absorption coefficient ( μ₃ ^ϊ> ) and a reduced scattering coefficient ^_s ^'b) which are optical constants of the specimen background are obtained. In a second

embodiment, an effective attenuation coefficient ^_eff) which is an optical constant of the specimen background is obtained. The second embodiment is preferable when the absorption coefficient and the Grueneisen coefficient of a light absorber in a phantom are not known.

[0089] In the second embodiment, a phantom and a

measurement system which are similar to those used in the first embodiment are used.

[0090] First, similarly to the first embodiment, the first generation unit 19a performs image reconstruction from received signals of photoacoustic waves so as to compute an initial sound pressure distribution, which is a

photoacoustic wave image. Then, the region extraction unit 19b which is a software program in the WS extracts regions that have a higher contrast from the obtained initial sound pressure distribution image, so that columnar light absorber images are specified.

[0091] Then, an objective function is defined as follows, and a proportional constant A and an effective attenuation coefficient μ^heff of the specimen background are used as variables and are obtained so that E is minimized.

[0092]

[Math. 6]

r

[0093] As a result, through the repeated calculation, an effective attenuation coefficient of the specimen background is obtained.

[0094] Furthermore, the second generation unit 19d uses this effective attenuation coefficient to compute the local fluence distribution in the phantom, and normalizes the initial sound pressure distribution image by using the local fluence distribution. Thus, an image in which an influence of the local fluence distribution is reduced can be obtained. Third Embodiment

[0095] An example for a specimen information acquisition apparatus using photoacoustic imaging to which the present invention is applied will be described with reference to Fig. 5. In the first and second embodiments, the optical

constants of the specimen background are obtained by using light absorbers in the specimen. In a third embodiment, the optical constants of the specimen background are obtained by using a light absorber disposed on the outside of the

specimen.

[0096] In the third embodiment, a Ti:sa laser which

produces pulsed light of 800-nm wavelength and about 15 nano-seconds is used as the light source 11. The energy of the light pulse emitted from the pulsed laser light 12 is 0.1 J. The pulsed light is expanded so as to have a radius of about 20 mm by using an optical system including a mirror and a beam expander. A different optical system is set in such a manner that a side surface of the specimen 15 is irradiated with the pulsed light 12 as illustrated in Fig. 5.

[0097] The same phantom as that used in the first

embodiment is used as the specimen 15. The base of the phantom is made from polyurethane rubber, and the optical constants are adjusted by using titanium oxide and ink. [0098] A sheet 51 of 2-mm thickness is disposed on the top surface of the base phantom and serves as a light absorber used for estimating the optical constants. This absorber has an absorption coefficient of 0.08 mm^-1 and a Grueneisen coefficient of 0.9. The detector 17 is in contact with the bottom of this phantom.

[0099] A two dimensional (2D) array detector having

multiple sensing elements arranged in two dimension is used as the detector 17. Then, produced photoacoustic waves are received by the sensing elements in the 2D array detector 17. The detected signals are converted into digital data for the photoacoustic signals by using the signal collector 18 which includes an amplifier, an A/D converter, and a FPGA. Then, the obtained digital data is transferred to the workstation (WS) which is the signal processing apparatus 19, and is stored in the WS .

[0100] Similarly to the first embodiment, the

photoacoustic image obtained through the program in the WS is analyzed, and an absorption coefficient and a reduced scattering coefficient which are average optical

characteristic values of the base phantom are computed. As a result, similarly to the first embodiment, values are obtained which are approximately equal to the optical

constants of the phantom. Then, the second generation unit 19d uses these values to compute the local optical fluence distribution in the phantom, and normalizes the initial sound pressure distribution image by using the local fluence distribution. Thus, an image in which an influence of the local fluence distribution is reduced can be obtained.

[0101] While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. The scope of the following claims is to be accorded the broadest interpretation so as to encompass all such modifications and equivalent structures and functions.

[0102] This application claims the benefit of Japanese Patent Application No. 2011-092155, filed April 18, 2011, which is hereby incorporated by reference herein in its entirety.

Reference Signs List

[0103] 19 signal processing apparatus

19a first generation unit

19b region extraction unit

19c optical constant estimation unit

19d second generation unit

Claims

[1] A specimen information acquisition apparatus

comprising :

a light source configured to irradiate a specimen with light;

a detector configured to detect an acoustic wave that is produced by the specimen due to the light; and

a signal processing apparatus configured to generate an optical characteristic value distribution of the specimen on the basis of a detected signal that is obtained from the detector,

wherein the signal processing apparatus includes

a region extraction unit configured to extract two or more regions that have a known absorption coefficient, from the optical characteristic value distribution of the specimen, and

an optical constant estimation unit configured to estimate an optical constant of a background of the specimen by using optical characteristic values of the two or more regions extracted by the region extraction unit and a local fluence distribution obtained from an optical constant that is set in advance for the background of the specimen.

[2] The specimen information acquisition apparatus

according to Claim 1, wherein the optical characteristic value is an initial sound pressure distribution or an optical energy absorption density distribution.

[3] The specimen information acquisition apparatus

according to Claim 1, wherein the optical constant of the background of the specimen is any of an absorption

coefficient, a scattering coefficient, an anisotropic

parameter, a reduced scattering coefficient, and an

effective attenuation coefficient.

[4] The specimen information acquisition apparatus

according to Claim 1, wherein the light absorber is a vessel.

[5] The specimen information acquisition apparatus

according to Claim 1, wherein the light absorber is provided on the outside of the specimen, and wherein the two or more regions are regions on the outside of the specimen.

[6] The specimen information acquisition apparatus

according to Claim 1, wherein the light absorber is a

contrast agent provided in the specimen.

[7] The specimen information acquisition apparatus

according to Claim 1, wherein the optical constant

estimation unit estimates an optical constant of the

background of the specimen by minimizing an objective

function that is defined on the basis of initial sound pressure distributions of the two or more regions and the optical constant that is set in advance.

[8] The specimen information acquisition apparatus

according to Claim 1, wherein the signal processing apparatus further includes a generation unit configured to compute a local fluence distribution of the specimen by using the optical constant of the background of the specimen that is estimated by the optical constant estimation unit, and to generate an optical characteristic value distribution of the specimen on the basis of the local fluence

distribution .

[9] A method used for a specimen information acquisition apparatus, the method comprising the steps of:

detecting an acoustic wave that is produced by a specimen due to light with which the specimen is irradiated; generating an optical characteristic value distribution of the specimen on the basis of a detected signal that is obtained in the detecting step;

extracting two or more regions that have a known absorption coefficient, from the optical characteristic value distribution of the specimen; and

estimating an optical constant of a background of the specimen by using optical characteristic values of the two or more regions and a local fluence distribution obtained from an optical constant that is set in advance for the background of the specimen.

[10] A program that causes a computer to execute the steps of:

generating an optical characteristic value distribution of a specimen on the basis of a detected signal that is obtained through detection of an acoustic wave that is produced by the specimen due to light with which the specimen is irradiated;