WO2012101652A2 - A novel process for synthesis of polyphenols - Google Patents
A novel process for synthesis of polyphenols Download PDFInfo
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- WO2012101652A2 WO2012101652A2 PCT/IN2012/000052 IN2012000052W WO2012101652A2 WO 2012101652 A2 WO2012101652 A2 WO 2012101652A2 IN 2012000052 W IN2012000052 W IN 2012000052W WO 2012101652 A2 WO2012101652 A2 WO 2012101652A2
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- UELLEKZKIHLEMJ-UHFFFAOYSA-N C(c1ccccc1)OC(C(c(cc1)cc(OCc2ccccc2)c1OCc1ccccc1)Oc1cc(OCc2ccccc2)c2)=Cc1c2OCc1ccccc1 Chemical compound C(c1ccccc1)OC(C(c(cc1)cc(OCc2ccccc2)c1OCc1ccccc1)Oc1cc(OCc2ccccc2)c2)=Cc1c2OCc1ccccc1 UELLEKZKIHLEMJ-UHFFFAOYSA-N 0.000 description 1
- NJKDDTOZBYEEMA-UHFFFAOYSA-N C(c1ccccc1)OC(C1)=C(c(cc2)cc(OCc3ccccc3)c2OCc2ccccc2)Oc2c1c(OCc1ccccc1)cc(OCc1ccccc1)c2 Chemical compound C(c1ccccc1)OC(C1)=C(c(cc2)cc(OCc3ccccc3)c2OCc2ccccc2)Oc2c1c(OCc1ccccc1)cc(OCc1ccccc1)c2 NJKDDTOZBYEEMA-UHFFFAOYSA-N 0.000 description 1
- CSQNIJRRXIHHAY-UHFFFAOYSA-N O=C1C(OCc2ccccc2)=C(c(cc2)cc(OCc3ccccc3)c2OCc2ccccc2)Oc2cc(OCc3ccccc3)cc(OCc3ccccc3)c12 Chemical compound O=C1C(OCc2ccccc2)=C(c(cc2)cc(OCc3ccccc3)c2OCc2ccccc2)Oc2cc(OCc3ccccc3)cc(OCc3ccccc3)c12 CSQNIJRRXIHHAY-UHFFFAOYSA-N 0.000 description 1
- 0 Oc1c(CSCO2)c2cc(C2I*2)c1 Chemical compound Oc1c(CSCO2)c2cc(C2I*2)c1 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/74—Benzo[b]pyrans, hydrogenated in the carbocyclic ring
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/30—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only not hydrogenated in the hetero ring, e.g. flavones
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
Definitions
- the present invention provides synthetic processes for preparing racemic and/or optically pure polyphenols and their variously functionalized derivatives.
- Polyphenolic natural products are of current interest because of their numerous biological activities, their widespread occurrence in foodstuffs, and their resulting relevance for human health. Polyphenolic natural products have one or several hydroxyl groups on their aromatic rings and often an additional hydroxyl group in the 3 position. Several different hydroxylation patterns of the A and B rings have been found in nature.
- Representative examples include: (-)- epiafzelechin, (+)-catechin, (-)-epicatechin, (-)-gallocatechin, (-)-epigallocatechin, their respective 3-gallate esters, as well as two 3-(30-methyl)gallate esters, which are referred to collectively herein as "catechins.”
- (+)-Catechin, (-)-catechin, (+)-epicatechin and (-)-epicatechin are flavan-3-ols, with (+)-catechin, (-)-epicatechin the most abundant.
- Catechins constitute about 25% of the dry weight of fresh tea leaves although the total content varies widely depending on tea variety and growth conditions.
- Catechins are also present in the human diet in chocolate, fruits, vegetables and wine. Catechins have found use in the treatment of acute coronary syndromes, including but not limited to myocardial infarction and angina; acute ischemic events in other organs and tissues, including but not limited to renal injury, renal ischemia and diseases of the aorta and its branches;. injuries arising from medical interventions, including but not limited to coronary artery bypass grafting (CABG) procedures and aneurysm repair; cancer; and metabolic diseases, including but not limited to diabetes mellitus.
- CABG coronary artery bypass grafting
- Health benefits of catechins have been broadly attributed to antioxidant properties, effects on intestinal microorganisms and nutrient absorption, and effects on metabolism and metabolic enzymes.
- Catechins for use as pharmaceutical and neutraceutical preparations have been obtained through plant extraction, followed if desired by purification of individual catechin species using chromatographic methods.
- comparisons must be made of defined structure prepared synthetically to polyphenols such as epicatechin. Synthetic monomers, dimers and oligomers are useful in various in vitro and ultimately in vivo models for pharmacological activity.
- An object of the invention is to provide a novel method of synthesis to obtain polyphenols in isomerically pure and/or racemic forms.
- the present invention relates to a novel synthetic process for preparing polyphenols or their derivatives both as racemic mixtures and enantiomerically pure forms of Formula (I) and their pharmaceutically acceptable salts.
- Y is selected from the group consisting of H and OR6;
- Rl, R2, R3, R4, R5, and R6 are independently selected from the group consisting of H, Ac, Bn, Allyl, propargyl, benzyl, 2-fluoroethyl, 4-nitrobenzyl, 4-chlorobenzyl, 4- methoxybenzyl, 4-methoxybenzonitrile, cinnamyl, methyl 4-crotonyl, but-2-en-l-yl, 2- pentenyl, (3-prop-len-lyl)sulfonyl benzene, l-trimethylsilyl-prop-l-yn-3-yl, 2-octyne-l- yl, 2-butyne-l-yl, 2-picolyl, 3-picolyl, 4-picolyl, quinolin-4-yl-methyl, acetonitrile, 2- methyl-oxirane, fluoromethyl, nitromethyl, methyl acetate-2-yl, methoxymethyl, acetamide,
- Formula (I) may be envisage to comprise the following compounds Fl to F4
- the present invention relates to a novel synthetic process for preparing polyphenols or their derivatives both as racemic mixtures and enantiomerically pure forms of Formula (I), their racemic mixtures, enantiomers, diastereomers and their pharmaceutically acceptable salts.
- Y is selected from the group consisting of H and OR6;
- Rl, R2, R3, R4, R5, and R6 are independently selected from the group consisting of H, Ac, Bn, Allyl, propargyl, benzyl, 2-fluoroethyl, 4-nitrobenzyl, 4-chlorobenzyl, 4- methoxybenzyl, 4-methoxybenzonitrile, cinnamyl, methyl 4-crotonyl, but-2-en-l-yl, 2- pentenyl, (3-prop-len-lyl)sulfonyl benzene, l-trimethylsilyl-prop-l-yn-3-yl, 2-octyne-l- yl, 2-butyne-l-yl, 2-picolyl, 3-picolyl, 4-picolyl, quinolin-4-yl-methyl, acetonitrile, 2- methyl-oxirane, fluoromethyl, nitromethyl, methyl acetate-2-yl, methoxymethyl, acetamide,
- Formula (I) may be envisage to comprise the following compounds Fl to F4
- the invention is directed to a novel method for preparing the compounds of Formula (I), or pharmaceutically acceptable salt(s) thereof.
- the process of this invention comprises one or more of the following steps, and illustrated in Scheme 1,
- the process of the present invention may yield pure formula (IV) or formula (V) or formula (VI) or a mixture of formula (IV) and (V).
- the reduction of the compound may be chiral or achiral, that may yield a compound of Formula (I) as a single enantiomer or an enantiomerically enriched mixture, for instance the compounds R,R diastereomer (Formula (VII)), the S,S diastereomer (Formula (VIII)), or a mixture thereof:
- the process of the present invention may yield compounds of Formula (I) such as (S,S)- epicatechin, (R,R)-epicatechin, a mixture of (S,S)- and (R,R)-epicatechin, (S,S)-epigallocatechin, (R,R)-epigallocatechin, and a mixture of (S,S)- and (R,R)-epigallocatechin.
- compounds of Formula (I) such as (S,S)- epicatechin, (R,R)-epicatechin, a mixture of (S,S)- and (R,R)-epicatechin, (S,S)-epigallocatechin, (R,R)-epigallocatechin, and a mixture of (S,S)- and (R,R)-epigallocatechin.
- the protected starting material [1] may be reduced to compounds such as [3] directly or through the intermediate [2] using suitable reducing agents such as lithium aluminum hydride in solvent such as tetrahydrofuran and followed by treatment with an acid such as hydrochloric acid.
- suitable reducing agents such as lithium aluminum hydride in solvent such as tetrahydrofuran and followed by treatment with an acid such as hydrochloric acid.
- the compound 3 may also be converted to [5a] and/ or [5b] using a reducing agent such as NaCNBH 3 in a solvent such as DCM.
- Further allylic alcohol [2] may be converted to 5a using reagent such as triethylsilane in solvent such as tetrahydrofuran at heating.
- [1] may also be selectively reduced to [4] in presence of a reagent such as lithium aluminum hydride with or without copper salt as a co-catalyst in solvent such as tetrahydrofuran at a temperature ranging from 60 - 80°C.
- a reagent such as lithium aluminum hydride with or without copper salt as a co-catalyst in solvent such as tetrahydrofuran at a temperature ranging from 60 - 80°C.
- racemic epicatechin or epigallocatechin [8] in major amount along with minor amount of catechin analogues.
- the racemic [8] can be resolved to pure enantiomer with the art known in literature such as enzymatic resolution using lipases/esterases and/ or chemical resolution by making diastereoisomers with a chiral acid.
- Achiral hydrogenation either of [3] or [5a] and/or [5b] in the presence of catalyst such as palladium on carbon in a solvent such as ethyl acetate under reducing atmosphere such as hydrogen can provide racemic [8].
- Chiral reduction either of [3] or [5a] and/or [5b] in the presence of a chiral reducing agent such as cinchona or other alkaloid-modified metal catalyst with a reducing atmosphere such as hydrogen in a solvent such as tetrahydrofuran can provide optically enriched or pure [6] or [7] as major products.
- the compounds of Formula(l), that contain more than one hydroxyl group may be protected by methods suitable for protecting hydroxyl groups.
- the compounds of Formula (I) may. be obtained by natural sources or synthetic sources and may be either anhydrous, hydrous or as a dihydrate.
- Suitable methods for protection of the hydroxyl group may be effected through alkylation, silylation, or esterification to form an ether, an ester, an acetate, a chloroacetate, a trifluoroacetate, a pivaloate, a benzoate, a 1,2- isopropylidene or a 1,3- isopropylidene.
- Alkylating agents include alkyi chlorides, bromides, iodides or alkyi sulfonates. Specific examples of alkylating agents include ally I bromide, propargyl bromide, benzyl bromide, 2-fluoroethyl bromide, 4- nitrobenzyl bromide, 4-chlorobenzyl bromide, 4-methoxybenzyl bromide, a-bromo-p- tolunitrile, cinnamyl bromide, methyl 4-bromocrotonate, crotyl bromide, l-bromo-2-pentene, 3-bromo-l-propenyl phenyl sulfone, 3-bromo-l-trimethylsilyl-l-propyne, 3-bromo-2-octyne, 1- bromo-2-butyne, 2-picolyl chloride, 3-picolyl chloride, 3-picolyl chloride, 3-
- a preferred reagent for alkylation may be a benzyl halide, such as benzyl bromide.
- the protection of the hydroxyl group by alkylation reaction may be carried out in the presence of a strong base and a polar organic solvent.
- the base may be selected from alkali metal hydride, dialkylamide, bis(trialkylsilyl)amide, carbonates or hydroxide, more preferably an alkali metal carbonate such as potassium carbonate.
- the solvent may be a polar water-miscible solvent selected from acetonitrile, tetrahydrofuran (tetrahydrofuran), dimethylacetamide, dioxane, ⁇ , ⁇ -dimethylformamide, a sulfoxide such as dimethylsulfoxide, or N-methylpyrrolidinone.
- the solvent is dimethylformamide.
- the alkylation reaction for the protection of the hydroxyl groups of quercetin [9] may be carried for a period of 4 - 7 hours, preferably at atmospheric pressure and at a temperature ranging from 60 -80 °C by reacting with [10] to yield the protected compound [11].
- the reduction of compounds of Formula (1) may be carried out by the use of suitable reducing agents such as metal hydride which may include sodium bis(2-methoxyethoxy)aluminum hydride, lithium aluminum hydride, sodium borohydride, aluminum hydride, diisobutyl aluminum hydride, trialkoxy aluminum hydride sodium amalgam, zinc mercury amalgam and sodium bis(2-methoxyethoxy)aluminum hydride.
- metal hydride which may include sodium bis(2-methoxyethoxy)aluminum hydride, lithium aluminum hydride, sodium borohydride, aluminum hydride, diisobutyl aluminum hydride, trialkoxy aluminum hydride sodium amalgam, zinc mercury amalgam and sodium bis(2-methoxyethoxy)aluminum hydride.
- the reducing agent is lithium aluminum hydride.
- the reduction may be carried out in the presence of lewis acids such as aluminum chloride, cerium chloride, zinc chloride, boron trifluoride, iodine.
- lewis acids such as aluminum chloride, cerium chloride, zinc chloride, boron trifluoride, iodine.
- the reduction reaction may be carried out in the presence of a solvent, which may be selected from the group comprising methyl tertiary butyl ether, tetrahydrofuran, diethyl ether, toluene, acetonitrile, etc.
- a solvent which may be selected from the group comprising methyl tertiary butyl ether, tetrahydrofuran, diethyl ether, toluene, acetonitrile, etc.
- the solvent is methyl tertiary butyl ether.
- the reaction may be carried out in a temperature ranging from -10 °C to 80 °C.
- the reduction and deprotection may be carried out by methods such that both reactions are carried out simultaneously.
- the hydrogenation catalyst is hydrogenation catalyst is selected from the group consisting of platinum, palladium, ruthenium, rhodium and nickel etc.
- a preferred catalyst is by the use of hydrogen in presence of 10 % palladium and carbon.
- Reaction is carried out in the presence of a solvent or mixture of solvents selected from methanol, ethanol, ethyl acetate, tetrahydrofuran, acetic acid, or their mixtures, where preferably the solvent is methanol, ethanol, ethylacetate or their mixtures.
- a solvent or mixture of solvents selected from methanol, ethanol, ethyl acetate, tetrahydrofuran, acetic acid, or their mixtures, where preferably the solvent is methanol, ethanol, ethylacetate or their mixtures.
- Reaction may be carried out at a temperature ranging from 25 - 60°C and at a pressure ranging from 4 - 50 psi.
- catechin may be prepared by the same process as claimed in this invention and is included within the scope of this invention.
- the present invention includes a process of converting the compound of Formula (VI) to the compound of Formula (IV), by the use of the reducing agent.
- the reducing agent may be selected from the group comprising metal hydrides which may include sodium bis(2-methoxyethoxy)aluminum hydride, lithium aluminum hydride, sodium borohydride, sodium cyanoborohydride, aluminum hydride, isobutyl aluminum hydride, trialkoxy aluminum hydride with or without Lewis acids such as aluminum chloride, cerium chloride, zinc chloride, boron trifluoride, or iodine.
- a suitable reducing reagent may include sodium amalgam, zinc mercury amalgam.
- the reducing agent is sodium cyanoborohydride.
- the reduction reaction may be carried in the presence of a solvent selected from the group consisting of methanol, ethanol, acetic acid, ethyl acetate, methyl t-butyl ether, diethyl ether, toluene, acetonitrile or tetrahydrofuran.
- the reaction may be carried out in the presence of sodium cyanoborohydride, acetic acid, and dichloromethane at a temperature of from 0 °C to 35 °C
- the unprotected or partially protected polyphenol may be resolved to obtain both the enantiomers in optically enriched form using asymmetric resolution technique such as:
- Racemic epicatechin may be resolved by methods such as enzymatic resolution, chemical resolution, chiral column chromatography and chiral induced fractional crystallization of either unprotected or selectively protected polyphenols, as mentioned above.
- the racemic polyphenol may be resolved by a method comprising the steps of: (i) protecting any four of the hydroxyl group of polyphenol by a suitable achiral protecting group, coupling the unprotected hydroxyl group with an optically pure acid to form an ester as a mixture of two diastereomers, more preferably esterification at 3 position by an optically pure -acid or activated acid to obtain an ester as a mixture of two diastereomers,
- step (ii) separation of the two diastereoisomers of step (i) by exploiting different chemical and/or physical properties of diastereoisomers ,such as fractional or preferential crystallization to obtain optically pure or diastereomerically enriched ester of preferred polyphenol,
- Alkylating agents include alkyl chlorides, bromides, iodides or alkyl sulfonates.
- alkylating agents include allyl bromide, propargyl bromide, benzyl bromide, 2-fluoroethyl bromide, 4-nitrobenzyl bromide, 4-chlorobenzyl bromide, 4- methoxybenzyl bromide, a-bromo-p-tolunitrile, cinnamyl bromide, methyl 4-bromocrotonate, crotyl bromide, l-bromo-2-pentene, 3-bromo-l-propenyl phenyl sulfone, 4-bromomethyl quinoline, bromoacetonitrile, bromofluoromethane, methyl bromoacetate, methoxymethyl chloride, bromoacetamide
- a suitable reagent for alkylation may be benzyl halide, such as benzyl bromide.
- the protection of the hydroxyl group by alkylation reaction is carried out in the presence of a base and a polar organic solvent.
- the base may be selected from the group comprising alkali metal hydride, dialkylamide, bis(trialkylsilyl)amide, carbonates or hydroxide, more preferably an alkali metal carbonate such as potassium carbonate.
- the solvent may be selected from the group comprising acetonitrile, tetrahydrofuran (tetrahydrofuran), ⁇ , ⁇ -dimethylformamide, a sulfoxide such as dimethylsulfoxide, or N- methylpyrrolidinone.
- the solvent is dimethylformamide.
- the alkylation reaction for the protection of the hydroxyl groups of epicatechin may be carried for a period of 4-7 hours preferably at atmospheric pressure and at a temperature ranging from25 - 80 °C.
- the protected polyphenol may be converted into its diastereoisomer by the use of chiral compounds selected from the group consisting of chiral tartaric acid or it's derivative, methoxyphenylacetic acid, 2-methoxy-2-(l-naphthyl)-propionic acid, etc.
- the chiral reagent is (S and/or /?)-2-methoxy-2-phenylacetic acid.
- the reaction may be carried out under general reaction conditions used for esterification where a hydroxyl group of polyphenol may' be esterified with a corresponding activated or un- activated acylating agent in a solvent such as tetrahydrofuran, dichloromethane, acetonitrile, dimethylformamide etc., in the presence of a base such as pyridine, triethyl amine, diisopropyl amine with or without the presence of a catalyst such 4-dimethylaminopyridine (DMAP) or ⁇ ', ⁇ '-dicylcohexylcarbodiimide (DCC) at a temperature ranging from 0 - 50 °C.
- a solvent such as tetrahydrofuran, dichloromethane, acetonitrile, dimethylformamide etc.
- a base such as pyridine, triethyl amine, diisopropyl amine with or without the presence of a catalyst such 4-dimethylamin
- the reaction conditions include the use of activated acid as acid chloride in dichloromethane as the solvent, triethyl amine as a base and 4-dimethylaminopyridine as a catalyst.
- the reaction may be preferably carried out at room temperature.
- the diastereoisomers may be separated by any method known in the art such as column chromatography, fractional crystallization etc. Preferably, the diastereoisomers are separated by fractional crystallization.
- Fractional crystallization may be achieved by solubilizing the compound in a single solvent in which the compound is freely soluble at higher temperature, but one of the diastereoisomers is insoluble at lower temperature, and thereby selectively precipitating one diastereoisomer and retaining the other in solution.
- Fractional crystallization may also be achieved by solubilizing the compound in a solvent or solvents in which the compound is freely soluble and adding another solvent or solvents in which one of the diastereoisomer is insoluble and thereby selectively precipitating one diastereoisomer and retaining the other in solution.
- the fractional crystallization of,the polyphenol stereoisomer may be achieved by the use of any suitable solvents.
- the fractional crystallization may be carried out solubilizing the compound in dichloromethane and precipitating the solution by methanol to selectively obtain one diastereomer in excess in the form of ester. This process may be repeated again to get a desire level of diastereomeric excess
- the chiral ester may be hydrolyzed by any method known in the art to obtain chiral purepolyphenol.
- a preferred method of hydrolyzing the chiral ester is by treating the diastereoisomerically pure ester with a base such as potassium carbonate in a solvent such as methanol and/or dichloromethane.
- optically pure benzyl protected polyphenol may be deprotected by general hydrogenolysis reaction conditions known in the art.
- This reaction may preferably be carried out in an atmosphere of hydrogen gas in a suitable organic solvent such as methanol, ethanol, ethyl acetate, tetrahydrofuran, acetic acid, etc. or mixtures thereof in the presence of a suitable catalyst such as palladium hydroxide, palladium on carbon at temperature range from room temperature to 60 °C.
- a suitable catalyst such as palladium hydroxide, palladium on carbon at temperature range from room temperature to 60 °C.
- the catalyst is palladium hydroxide and solvent is ethyl acetate at room temperature under a hydrogen atmosphere .
- the protected polyphenol may be converted to an ester by esterification, using acids or acid chlorides, such as alkyl or aryl esters.
- the enzymatic separation reaction may be carried out under general enzyme hydrolysis conditions known in the art used for resolving prochiral esters, for example using commercial esterases with a mixture of water-miscible or immiscible solvents such as tetrahydrofuran, acetonitrile, dimethylformamide, or ethyl acetate, etc., in the presence of base addition so as to maintain a pH range where the enzyme is still active.
- the reaction may be carried out at room temperature.
- the resulting chiral hydrolyzed protected enantiomer of polyphenol may be separated from the unreacted ester by any method known in the art such as column chromatography, fractional crystallization etc.
- the process of the present invention as may be directed to a novel method for preparing epicatechin and epigallocatechin, or pharmaceutically acceptable salt(s) thereof.
- the process for preparing epicatechin and epigallocatechin comprises one or more of the following steps, and illustrated in Scheme 1: (i) partially or fully and/or selectively protecting the hydroxyl groups of quercetin or myricetin with one or more protecting groups;
- Quercetin may be conducted by a process as illustrated in Sche
- the protected quercetin [11] (pentabenzyl quercetin; 3,5,7-tris(benzyloxy)-2-(3,4- bis(benzyloxy)phenyl)-4H-chromen-4-one) is reduced by suitable reducing agents as described above to obtain protected 4H chromene [12] 3,5,7-tris(benzyloxy)-2-(3,4-bis(benzyloxy)phenyl)- 4H-chromene...
- racemic epicatechin [13] by reduction and deprotection of 4H chromene is illustrated in Scheme 5.
- the protected 4H chromene [12] may be simultaneously reduced and deprotected or sequentially reduced and de-protected as shown in the general synthetic Scheme 5.
- the reduction of chromene may be carried out in the presence of a chiral catalyst and/or chiral auxiliary known in the art, to provide isomerically enriched reduced product.
- the preferred method is the simultaneous reduction and deprotection of cyanidin.
- This reaction may preferably be carried out in an atmosphere of hydrogen gas in a suitable organic solvent such as methanol, ethanol, ethyl acetate, tetrahydrofuran, acetic acid etc. or mixtures thereof in the presence of a suitable catalyst such as Pd, Pt, Ni etc. adsorbed onto a solid support.
- the reduction and protection of 4 H chromene may be carried out by methods as above.
- the protected quercetin [11] penentabenzyl quercetin; 3,5,7-tris(benzyloxy)-2-(3,4- bis(benzyloxy)phenyl)-4H-chromen-4-one
- suitable reducing agents to obtain protected cyanidin [14] (3,5,7-tris(benzyloxy)-2-(3,4-bis(benzyloxy)phenyl)chromenylium).
- the conversion of cyanidin is illustrated in scheme 7.
- the protected cyanidin can be converted to of 4H chromene and 2H chromene using a suitable reducing agent. Further, reaction conditions can be optimized to get either 4H chromene or 2H chromene as an exclusive product.
- cyanidin [14] may be converted to 2H chromene [16] and 4 H chromene [12] by reduction.
- the reducing agent may be selected from the group consisting of metal hydrides which may include sodium bis(2-methoxyethoxy)aluminum hydride, lithium aluminum hydride, sodium borohydride, sodium cyanoborohydride, aluminum hydride, isobutyl aluminum hydride, trialkoxy aluminum hydride with or without Lewis acids such as aluminum chloride, cerium chloride, zinc chloride, boron trifluoride, or iodine.
- a suitable reducing reagent may include sodium amalgam, zinc mercury amalgam, preferbaly sodium cyanoborohydride.
- the reaction may be conducted in the presence of a solvent selected from the group consisting of methanol, ethanol, acetic acid, ethyl acetate, methyl t-butyl ether, diethyl ether, toluene, acetonitrile or tetrahydrofuran or their mixture.
- a solvent selected from the group consisting of methanol, ethanol, acetic acid, ethyl acetate, methyl t-butyl ether, diethyl ether, toluene, acetonitrile or tetrahydrofuran or their mixture.
- the solvent is a mixture of acetic acid and dichloromethane at a temperature of from 0 °C to 35 °C Conversion of 2H chromene to 4H chromene
- 4H chromene to 2H chromene may be converted to 2 H chromene as below at Scheme 8.
- 2H chromene may be converted to 4H chromene by the use of a catalyst.
- Catalyst may be a lewis acid such as aluminum chloride, cerium chloride, zinc chloride, boron trifluoride and/ or iodine or a mild acid such as para-toluenesulfonic acid in a solvent such as THF, toluene, xylene, nitrobenzene etc at a temperature ranging from 20 to 150 oC.
- a lewis acid such as aluminum chloride, cerium chloride, zinc chloride, boron trifluoride and/ or iodine
- a mild acid such as para-toluenesulfonic acid in a solvent such as THF, toluene, xylene, nitrobenzene etc at a temperature ranging from 20 to 150 oC.
- the unprotected or partially protected racemic epicatechin and/or epigallocatechin or related phenols can be resolved to get both the enantiomers in optically enriched form using asymmetric resolution technique known in the art such as:
- the product is the ester of the desired isomer
- the final product in this instance epicatechin or epigallocatechin can be obtained by the hydrolysis of the ester moiety by acid catalyzed hydrolysis or base catalyzed hydrolysis by methods Resolution of compounds of the present invention
- the compounds of the present invention may be reloved by the method comprising the steps of:
- step (iii) separation of the two diastereoisomers formed in step (ii) by fractional or preferential crystallization to obtain an optically pure or diastereomerically enriched ester;
- the resolution may be carried out such that the esterification occurs at step (v)
- the racemic epicatechin may be resolved by any method known in the art such as enzymatic resolution, chemical resolution, chiral column chromatography and chiral induced fractional crystallization of either unprotected or selectively protected epicatechin, as mentioned above.
- the compound of formula (II) is a racemic quercetin or myricetin.
- racemic myricetin or quercetin may be carried out by:
- the protection at step (i) of the resolution of quercetin and myricetin may be
- the esterification may be carried out preferably at 3 position by an optically pure -acid or activated acid to obtain an ester as a mixture of two diastereomers.
- the protected epicatechin may be converted to its diastereoisomer by the use of chiral compounds selected from the group consisting of chiral tartaric acid or it's derivative, methoxyphenylacetic acid, 2-methoxy-2-(l-naphthyl)-propionic acid, etc.
- the chiral reagent is (S and/or R)-2-methoxy-2-phenylacetic acid.
- the diastereoisomers may be separated by any method known in the art such as column chromatography, fractional crystallization etc. Preferably, the diastereoisomers are separated by fractional crystallization. Hydrolysis of the Chiral Ester
- the chiral ester may be hydrolyzed by any method known in the art to obtain chiral pure epicatechin.
- a preferred method of hydrolyzing the chiral ester may be by treating the diastereoisomerically pure ester with a base such as potassium carbonate in a solvent such as methanol and/or dichloromethane.
- optically pure benzyl protected epicatechin may be deprotected by general hydrogenolysis reaction conditions known in the art.
- the protected epicatechin may be converted to an ester by esterification, using acids or acid chlorides, such as alkyl or aryl esters.
- the present invention is drawn to the intermediates of the novel process of synthesis of polyphenols.
- this aspect discloses the unprotected, partially protected and completely protected 4H chromenefformula (II)] and 2H chromene[formula (III)] structures of as below:
- R is selected from H, CH 3 , Bn, Ac, Si(CH 3 ) 3 , allyl.
- the present invention is drawn to the use of 4H chromene and 2H chromene in the synthesis of epicatechin.
- the invention is directed to methods of preparing pharmaceutical or nutraceutical compositions comprising catechin and/ or epicatechin. These methods comprise preparing catechin and/ or epicatechin, or pharmaceutically acceptable salt(s) thereof, by the methods described herein and combining this with a pharmaceutically or neutraceutially acceptable carrier.
- the invention is directed to methods of administering such a pharmaceutical or nutraceutical composition to a subject in need thereof.
- Routes of administration for the pharmaceutical and nutraceutical compositions of the present invention include parenteral and enteral routes.
- Preferred enteral routes of administration include delivery by mouth (oral), nasal, rectal, and vaginal routes.
- Preferred parenteral routes of administration include intravenous, intramuscular, subcutaneous, and intraperitoneal routes.
- the pharmaceutical or nutraceutical compositions of the present invention are administered in an "effective amount.”
- an "effective amount” is not limited to a minimal amount sufficient to ameliorate a condition, or to an amount that results in an optimal or a maximal amelioration of the condition.
- an effective amount of one such pharmaceutical may not be, in and of itself be an effective amount, but may be an effective amount when used together with additional pharmaceuticals.
- the semi solid obtained was triturated with 50% ethyl acetate: hexane (250 ml) for 30 min at room temperature yielding a solid.
- the solid was further filtered and washed with 50% ethyl acetate: hexane (200ml).
- the solid was dried under vacuum to obtain the product as an off-white solid (0.250g, 10%) [16].
- Example 6 Synthesis of racemic epicatechin and racemic catechin from 2H-Chromene [16]
- Example 7 Synthesis of 4H-chromene [16] and/or 2H-chromene [14] from cyanidin:
- [(5)-2-methoxy-2-phenylacetyl chloride [20] was separately prepared by stirring (S)-2-methoxy-2-phenylacetic acid and thionylchloride in dry dichloromethane with catalytic amount of dimethylformamide for 30 min. and removing the excess thionylchloride and dichloromethane by under vacuum]. After the addition, the reaction mixture was stirred at 40 °C for 5 to 6 hours and monitored by TLC. On complete consumption of the starting material, the reaction mixture was cooled to ambient temperature and the organic layer was washed with water followed by brine solution. The organic layer was dried over anhydrous sodium sulfate and concentrated under vacuum to yield 0.500 g of a thick viscous semi solid.
- Example 16 Chiral Preparative HPLC Resolution of Racemic Epicatechin/catechin:
- the racemic mixture of epicatechin was dissolved in methanol and checked for its chiral purity on reverse phase CHIRAL PAK ® IC (250 X 4.6) mm, 5 ⁇ column at 25 0 C temperature.
- the mobile phase used was hexanes/ ethanol/ trifluoroacetic acid// 60/ 40/ 0.05 (v/v/v) with a flow rate of 1.0 ml/minute and sample injection volume of 10 ⁇ .
- the signals were monitored at UV 280 nm with PDA.
- the both isomers separated with a retention time difference of about 1.6 minutes.
- the faster moving isomer on HPLC eluted at 4.7 minute while the slower moving isomer came at 6.3 minute on a 15 minute run.
- the racemic mixture (0.200 g) was dissolved in methanol and separated on a preparative HPLC on CHIRAL PAK ® IC (250 X 20) mm column at 25 0 C temperature.
- the sample injection volume was 2.0 ml with a feed concentration of 5 mg/ml.
- the mobile phase used was Hexanes/ EtOH// 60/40 v/v with a flow rate of 18 ml/ minute.
- the detection was done at UV 280 nm with PDA.
- the faster moving epicatechin isomer I (0.085 g) eluted at 4.7 minute and the slower moving epicatechin isomer II (0.084 g) at 6.3 minute with a qualitative purity of 99.6% and 99.8 % respectively.
- the slow moving isomer eluted at 6.3 minutes was assigned to be (-)-epicatechin ((2 ?,3/?)-2-(3,4-Dihydroxyphenyl)-3,4-dihydro-l(2H)-benzopyran- 3,5,7-triol) isomer and the fast moving isomer which eluted at 4.7 minutes was assigned to be (+)-epicatechin (((2S,3S)-2-(3,4-Dihydroxyphenyl)-3,4-dihydro-l(2W)-benzopyran-3,5,7-triol) isomer.
Abstract
Description
Claims
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JP2013551012A JP5890846B2 (en) | 2011-01-27 | 2012-01-24 | New synthesis method of polyphenol |
ES12739384.1T ES2636441T3 (en) | 2011-01-27 | 2012-01-24 | A novel procedure for the synthesis of polyphenols |
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WO2014115174A2 (en) | 2013-01-26 | 2014-07-31 | Sphaera Pharma Pvt. Ltd. | Novel approach for synthesis of catechins |
WO2014162320A2 (en) | 2013-04-04 | 2014-10-09 | Sphaera Pharma Pvt. Ltd. | Novel analogues of epicatechin and related polyphenols |
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US10618933B2 (en) | 2014-07-23 | 2020-04-14 | Epirium Bio Inc. | Hydroxysteroid compounds, their intermediates, process of preparation, composition and uses thereof |
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CN106946835B (en) * | 2017-04-25 | 2019-03-01 | 广州科技职业技术学院 | Hesperetin carboxylate and its preparation method and application |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2122608B (en) | 1982-06-01 | 1985-10-02 | Zyma Sa | (+)-cyanidan-3-ol derivatives |
DE69132694T2 (en) * | 1990-03-08 | 2002-06-20 | Fuji Photo Film Co Ltd | Positive working photoresist composition |
US6476241B1 (en) * | 2000-09-05 | 2002-11-05 | Mars Incorporated | Synthesis of 4α-arylepicatechins |
JPWO2004078741A1 (en) * | 2003-03-03 | 2006-06-08 | 三栄源エフ・エフ・アイ株式会社 | Adiponectin expression promoter |
JP5339664B2 (en) * | 2004-02-13 | 2013-11-13 | 花王株式会社 | AMPK activator |
JP2006083105A (en) * | 2004-09-16 | 2006-03-30 | Ito En Ltd | Ameliorant for blood fluidity and food and beverage |
CA2612438A1 (en) * | 2005-06-29 | 2007-01-04 | Mars, Incorporated | Preparation of (+)-catechin, (-)-epicatechin, (-)-catechin, (+)-epicatechin, and their 5,7,3',4'-tetra-o-benzyl analogues |
EP2167043A4 (en) * | 2007-06-06 | 2013-05-01 | Univ South Florida | Nutraceutical co-crystal compositions |
JP5382676B2 (en) * | 2007-12-07 | 2014-01-08 | 国立大学法人名古屋大学 | Method for producing anthocyanidins |
-
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US9428482B2 (en) | 2016-08-30 |
JP5890846B2 (en) | 2016-03-22 |
TWI697489B (en) | 2020-07-01 |
ES2636441T3 (en) | 2017-10-05 |
WO2012101652A3 (en) | 2012-12-27 |
CN103582635A (en) | 2014-02-12 |
CN103582635B (en) | 2019-02-19 |
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US20140031421A1 (en) | 2014-01-30 |
EP2668176A2 (en) | 2013-12-04 |
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