WO2012089115A1

WO2012089115A1 - Moisture-retaining chinese herbal medicine composition and preparation method and use thereof

Info

Publication number: WO2012089115A1
Application number: PCT/CN2011/084772
Authority: WO
Inventors: 程康; 陈默; 祝乐; 熊卫国; 赵亚; 方程; 吕洛; 魏少敏
Original assignee: 上海家化联合股份有限公司
Priority date: 2010-12-31
Filing date: 2011-12-27
Publication date: 2012-07-05
Also published as: CA2823423A1; CA2823423C; AU2011352504B2; CN102552080B; CN102552080A

Abstract

Disclosed is a Chinese herbal medicine composition having a moisture-retaining effect, containing water and/or solvent extracts of raw materials in the following parts by weight: 20-50 Asparagus cochinchinensis, 20-50 rehmannia root, 10-40 Dendrobium nobile, and 10-40 Dioscorea opposita. The composition can assist skin against drying in a dry environment, and prevent moisture loss.

Description

Moisturizing Chinese herbal medicine composition and preparation method and application thereof

Technical field

The present invention relates to the field of cosmetics, and in particular to a herbal extract and its preparation and use in cosmetics, and a moisturizing herbal composition comprising the Chinese herbal extract and its preparation and application in cosmetics. Background technique

The skin consists of epidermis, dermis and subcutaneous fat tissue, each layer containing water. The amount of water in the skin is the key to the tenderness, fullness, luster and softness of the skin. Infants and young children have high water content and appear delicate and lustrous. The skin of the elderly is dry and wilting due to low water content, easy to desquamation, and even wrinkles.

Moisturizing is the eternal theme. It is an important condition for ensuring healthy skin and delaying aging. It is a desirable goal to develop and research moisturizers with excellent performance.

On the one hand, with the increase of age, the skin moisturizing mechanism is damaged, the moisture content of the skin tissue cells and cells is reduced, and when the water in the stratum corneum drops below 10%, the skin will appear dry, lose elasticity, wrinkle and accelerate skin aging. . On the other hand, the skin is exposed to heat, cold, wind, detergent and ultraviolet radiation, and the surface becomes drier. Therefore, how to maintain the moisture of the skin and reduce the loss of water is very important. Currently, skin moisturizers on the market mainly include a blocking agent, a moisture absorbent, a hydrophilic matrix, a light inhibitor and some special additives.

Traditional medicine believes that dry skin is caused by dysfunction of the lungs and kidneys, imbalance of water and liquid metabolism, and fluid is not good for the skin. Therefore, the way to moisturize the skin is to supplement the lungs and kidneys and nourish the body fluid. In the Chinese medical literature, there are also records of moisturizing skin, and a collection of Chinese medicine beauty prescriptions, from theory to practice. In recent years, some modern studies have explained the role of polysaccharides. The perfect combination of high water absorption and good film-forming properties of polysaccharides can achieve a good moisturizing effect. Plant polysaccharides are gradually being applied to skin moisturizing, while polysaccharides are mostly The main ingredients contained in Chinese herbal medicines have brought us bright prospects for finding safe and effective skin moisturizers. Summary of the invention

The technical problem to be solved by the present invention is to provide a Chinese herbal medicine extract having a moisturizing effect. In order to achieve the above object, the Chinese herbal medicine extract of the present invention is a water and/or solvent extract of the following Chinese herbal medicines: Asparagus: 20-50, Rehmannia glutinosa: 20~50, Dendrobium: 10~40, Yam: 10~ 40. The solvent is selected from the group consisting of methanol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1, 3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof. . Root, also known as Asparagus. Excavate in the autumn and winter seasons, wash, remove the stem base and fibrous roots, boil in boiling water or steam to the heart, remove the skin while hot, wash and dry. Asparagus is mainly distributed in South China, Southwest China, Central China and Henan, Shandong and other provinces.

Tianmendong was born in the highlands, with whitening and moistening, clearing the lungs, strengthening the kidneys, nourishing the yin and moistening the skin, and nourishing the skin. The rehmannia is produced in the thickest part of the Central Plains. The color is yellow and sweet, and the earth is righteous. It is thick and heavy, and has the strongest flavor. It is a multi-fat cream, so it is a good nourishing agent. Fresh is the new radix rehmannia, because of the rustic, to the yin nature. For example, "The Herbal Enlightenment" said: "The solidification of the land of the yellow land, the right color of the soil, the medicine for the kidney, the top of the Yin." The two herbs are rising and falling, one day and one place, containing the aura of heaven and earth. Therefore, the "Compendium of Materia Medica" contains the combination of Tianmendong and Rehmannia glutinosa, and the marbles are large. Each of the warm wines is made of three pills, three times, and the mountains are far away, and the valley is good. It is served for ten days. Ming, to the 20th, a hundred times healed, the color is like a flower; on the 30th, the whitening is darker, the tooth is reborn, the 50th, the line and the horse, the hundred days, the year, this is the heaven and earth soup. The reason why it can nourish and moisturize, because both drugs are into the lung and kidney, supplement the lungs, nourish the kidney water, regulate the metabolism of the body fluid, make the skin and hair moist.

Rehmannia refers to the medicinal part of the root of plant rehmannia. Rehmannia glutinosa is the dry root of Rehmannia glutinosa Libosch. Excavation in the autumn, remove the reed, the roots and the sediment, slowly bake the ground yellow to about 80%. Rehmannia is mainly distributed in Liaoning, Hebei, Henan, Shandong, Shanxi, Shaanxi, Gansu, Inner Mongolia, Jiangsu, Hubei and other provinces.

Caulis Dendrobi refers to the medicinal part of the fresh or dry stem of the plant sarcophagus, which is a perennial epiphytic herb of the genus Orchidaceae. It is mainly distributed in Yunnan, Sichuan, Guizhou and tropical regions of Asia. It can be harvested all year round and harvested in the fall. Dry or dry, cut into sections, and use.

Dendrobium: Gan Han, Guiwei Shenyin, Yiwei Shengjin, Ziyin Qingre, Runfei Yishen, used for Yin and Jin deficiency, mouth and polydipsia; "Shen Nong's Herbal Classic""complementing five dirty and sturdy, thin and light "Year of the famous doctor";"The muscles of the stone are long, the skin is evil and the heat is suffocating";"MateriaMedica" cloud "the stone is round and thin, and it is said that it is good and strong, strong and tonic, strong knees, Drive cold, but horrified, set mind.., Xu Yuren: "Shijie function clear stomach and stomach, stomach and kidney heat is best. If you want to clear your stomach and save your life, you will not be able to use it. For merit. " Modern pharmacological research shows that the active constituents of Dendrobium contain alkaloids, polysaccharides, trace elements and amino acids, and their extracts have excellent water holding effect and can increase capillary blood flow; and can significantly increase superoxide dismutase (SOD) level, which plays a role in reducing lipid peroxide (LPO); from the perspective of the regulation of brain monoamine neurotransmitter levels, it acts as an inhibitor of monoamine oxidase (MAO) and acts as an anti-aging agent.

Yam refers to the medicinal part of the dried rhizome of the plant Dioscorea. Yam is a yam tree Dioscorea opposita Thunb. j dry H, . After the winter leaves and leaves are withered, the roots are removed, the roots are removed, washed, the outer skin and fibrous roots are removed, and dried. Yam is now distributed in various provinces and regions in the north, northwest and Yangtze River basins of China.

Yam is the best. Shengjin emollient, "Compendium": "Yi Shen Qi, Jianpi Stomach, Stop sputum, phlegm, moisturizing hair." Li Wei: "It also cures dry skin, so that it is moist." "Materia Medica": "Yam, this is a food, the ancients used to add decoction, which means that it can replenish the spleen and replenish heat, but the gas is warm but flat, to replenish the spleen and lungs, it is able to moisturize the skin, long muscles." Because of its warmth and coolness, it can replenish the lungs and nourish the kidneys and supplement the spleen and stomach. It is a supreme product in nourishing medicines, and its characteristics are very peaceful.

The four herbs in this side were listed as top grades in the Shennong Bencao Classics more than 2,000 years ago. Among them, Tianmendong nourishing yin and moistening dryness and nourishing heart and lungs, rehmannia radix rehmanniae nourishing and nourishing liver and kidney, using Tianmendong and raw land compared to the world, taking the gas of ascending and descending, Shangruntou face heart and lung qi and blood, lowering liver and kidney essence, but Junshi; Shiqi nursed the spleen and stomach to nourish yin and spleen, as a medicine, can replenish the soil spleen and stomach, nourish the skin and moisturize the skin, yam Yiwei Shengjin emollient, for the adjuvant to strengthen the effect of Yiwei Shengjin emollient, four drugs with the same, five internal organs Co-adjust, soften the skin, clear and replenish. Combined with a large number of ancient beauty literature prescriptions, the formula was adjusted by trial and error.

Preferably, in the Chinese herbal medicine extract of the present invention, the extraction is selected from the group consisting of boiling, reflux extraction, impregnation, percolation, or a combination thereof.

Preferably, the form of the extract of the present invention is selected from the group consisting of a solution, a powder, an ointment, a film or a cream.

The invention also provides a preferred preparation method of a Chinese herbal medicine extract, the method comprising:

The following parts by weight: Asparagus: 20-50, Rehmannia glutinosa: 20 50, Dendrobium: 10~40, Yam: 10~40 of Chinese herbal medicine is thoroughly mixed; soaked in water or solvent for 0.5~1 hour and extracted, the solvent selected Yue, ethanol, isopropanol, n-butanol, ethylene glycol, 1, ² _ propylene glycol, 1, ³ _-propanediol, 1,3-butanediol, acetone, ethyl acetate, or combinations thereof.

Preferably, the method further comprises:

Filtering, and repeatedly extracting the filter residue, and combining the filtrates; The filtrate is concentrated to 2 times the amount (V/W) of the Chinese herbal medicine, and the multiple is the ratio of the volume of the solvent to the total weight of the medicinal material, wherein the unit of the solvent volume is ml, and the unit of the total weight of the medicinal material is g;

Then carry out alcohol precipitation;

The alcohol precipitation supernatant recovers the solvent.

The alcohol precipitate can be formulated into a desired dosage form, such as a solution, a powder, an ointment, a film or a cream.

Preferably, in the above preparation method, the extracted Chinese herbal medicine is extracted by boiling, refluxing, dipping or osmosis.

Preferably, the alcohol precipitation employs an alcohol selected from the group consisting of decyl alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propylene glycol, 1, 3-propanediol, 1, 3-butanediol, or combinations thereof.

The Chinese herbal medicine extract of the invention has good moisturizing effect and can be applied to cosmetics, in particular, it is suitable for cosmetics such as beauty creams, eye creams, facial masks, facial cleansers, toners and essences.

The Chinese herbal medicine extract of the invention can be directly used for skin moisturizing, and can also be used as an effect additive to be added into cosmetics, which can help the skin resist dryness in a dry environment, prevent water from dispersing, moisturize and skin, and the skin is radiant and moist, and has no skin. Stimulating, and at a lower cost. DRAWINGS

Figure 1 A shows the state of the cells at 0 min after drying in the blank control group without incubation.

Figure 1B shows the state of the cells when the cells were not incubated for 15 minutes.

Fig. 2A shows the state of the cells when the cells incubated with the extract of Example 1 were dried for 0 minutes. Fig. 2B shows the state of the cells when the cells incubated with the extract of Example 1 were dried for 15 minutes. Fig. 3A is the state of the cells when the cells incubated with the extract C of Example 3 were dried for 0 minutes. Fig. 3B is the state of the cells when the cells incubated with the extract C of Example 3 were dried for 15 minutes. Fig. 4A is a view showing the state of the cells when the cells incubated with the extract D of Example 4 were dried for 0 minutes. Fig. 4B is the state of the cells when the cells incubated with the extract D of Example 4 were dried for 15 minutes.

detailed description

The invention will now be described in greater detail by way of specific examples.

Example 1 Take pure Chinese herbal medicine asparagus 30g, rehmannia root 30g, sarcophagus 10g, yam 30g total 100g, mix well, add 10 times the amount (multiple amount: refers to the ratio of solvent volume to the total weight of the medicinal material, where the unit volume of the solvent is ml, medicinal materials The unit of total weight is g, the same as) 75% ethanol (v / v) cold soak for 48 hours, filtered, the filtrate is recovered ethanol, the resulting extract is dissolved in water to prepare a solution containing 1000 mg / ml of crude drug, which is extracted. Liquid A. Example 2

Take pure Chinese herbal medicine Asparagus 30g, Rehmannia glutinosa 50g, Dendrobium 10g, Yam 10g total 100g, mix well, add 10 times methanol to reflux extraction for 2 hours, filter, the filtrate recovers sterol, the obtained extract is dissolved in water, made into A solution having a crude drug amount of 50 mg/ml was used to obtain an extract B. Example 3

Take pure Chinese herbal medicine Asparagus 50g, Radix Rehmanniae 30g, Dendrobium 10g, Yam 10g total 100g, mix well, decoction and extract with 10 times of water for about 2 hours, concentrate, filter, add supernatant to add raw water A solution of 1000 mg/ml gave an extract (: Example 4.

Take pure Chinese herbal medicine asparagus 30g, rehmannia root 30g, sarcophagus 30g, yam 10g total 100g, mix well, decoction and extract with 10 times water for about 2 hours, concentrate, add 2 times ethanol precipitation, filter, recover ethanol from filtrate The obtained extract was dissolved in water and filtered to prepare a solution containing a crude drug amount of 500 mg/ml to obtain an extract D. Test Example 1 In vitro thermogravimetric analysis test:

Thermogravimetric analysis (TG) is a technique for measuring the mass change of a substance with temperature and time under a program temperature control by a thermogravimetric analysis instrument, which can reflect changes in water evaporation.

Equal masses of water, 10% of Example 1 Extract A, Example ² Extract B, Example 3 Extract C, and Example 4 Extract D were placed under the same temperature environment, and different time periods were measured by a thermogravimeter. The quality of the change. At the same time point, the higher the residual ratio, the stronger the ability to combine water to prevent water dispersal, and the better the moisturizing effect. The test results are shown in Table 1.

Table 1

Test Example 2 In vitro test for preventing keratinocyte water loss

According to the moisturizing-related mechanism, the in vitro evaluation method of the cell platform was used, and in the dry environment of the epidermal cells, the extracts of the examples were examined to help the cells resist drying, prevent water loss, and maintain the original morphology of the cells.

0.1 ml of the extract of each example was taken, and 9.9 ml of a medium (MEM) of MEM (Minimum Essential Media) was added thereto, and suction-filtered to prepare a concentration of 1%. In the same way, 1% glycerol was prepared as a positive control. The human epidermal keratinocyte Hacat was selected as the experimental cells, the cells cultured in the normal MEM solution were used as the blank control group, and the cells cultured in the MEM containing 1% glycerol were used as the positive control group, and the extract containing the sample of 1% concentration was used. The cells cultured in MEM were the dosing group. After incubation for 24 h, the liquid in each well was blotted in turn, placed under a microscope (10x20) to observe the time from normal to dry (significant change in state), and photographed at 15 min. See Figure 1 A-5B for details.

Experimental results:

Under the dry conditions given by the outside, the blank group cells incubated without the extract of the example gradually lost water, and the drying time of the unloading time was 720 seconds. The positive control group incubated with 1% glycerol maintained the drying time relative to the blank group. The increase is 780 seconds, and the keratinocytes incubated with the extracts of the examples can help the skin cells to maintain moisture, give the cells a good resistance to drying, and can withstand drying time of 780 seconds or more, which is more than 9% more than the blank group. , the effect is more than 1% glycerol control, see the following table:

Table 2

Average Dry Maintenance Time (seconds) Difference Percentage Blank Control 720

1% glycerol 780 60 8.33% Example 1 Extract A 810 90 12.5% Example 2 Extract B 785 65 9.02% Example 3 Extract C 805 85 11.81% Example 4 Extract D 830 110 15.28%

At the 15th minute of drying, the cells in the blank group had shrunk and severely lost water, the refractive index increased, and the morphology changed significantly. However, the cells in the incubation group of each example maintained the original morphology, indicating that the extracts of the examples have good properties. It has anti-drying and moisturizing effect, and the anti-drying and moisturizing effect of the extract D of the embodiment 4 is the best, and the percentage of the drying time is up to 15.28%.

From the above experimental studies, it has been revealed that the Chinese herbal medicine composition of this embodiment is capable of imparting a good anti-drying ability to skin keratinocytes, reducing water loss and helping the skin to remain moist.

Claims

Claim

1. A Chinese herbal medicine extract, which is a water and/or solvent extract of a raw material consisting of the following parts by weight: Asparagus: 20-50, Rehmannia glutinosa: 20~50, Dendrobium: 10~40, Yam: 10~40 . The solvent is selected from the group consisting of decyl alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1, 2-propanediol, 1,3-propanediol, 1, 3-butanediol, acetone, ethyl acetate or a combination thereof .

The herbal extract according to claim 1, wherein the extraction is by boiling, refluxing, dipping or osmosis.

The herbal extract according to claim 1 or 2, wherein the herbal extract form is selected from the group consisting of a solution, a powder, an ointment, a film or a cream.

A Chinese herbal medicine composition having a moisturizing action, comprising the herbal extract of any one of claims 1 to 3.

5. Use of a Chinese herbal composition according to claim 3 in cosmetics.

6. A method for preparing a Chinese herbal medicine extract, comprising the steps of:

It will be divided into the following parts by weight: Asparagus: 20-50, Rehmannia glutinosa: 20~50, Dendrobium: 10~40, Yam: 10~40, and the raw materials are soaked with water and/or solvent for 0.5~1 hour and extracted. It is selected from the group consisting of methanol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1,3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof.

7. The method according to claim 6, further comprising:

Filtering, and repeatedly extracting the filter residue, and combining the filtrates;

The filtrate is concentrated to about twice the total amount of the medicinal material, and the multiple is the ratio of the volume of the solvent to the total weight of the medicinal material, wherein the unit of the solvent volume is ml, and the unit of the total weight of the medicinal material is g;

Then carry out alcohol precipitation ^

The alcohol precipitation supernatant recovers the solvent.

The method according to claim 6 or 7, wherein the alcohol precipitation is selected from the group consisting of decyl alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1 . 1,3-propanediol, 1, ³ - butylene glycol, or a combination of alcohol.

9. A herbal extract prepared by the method of claim 6 or 7.

The Chinese herbal medicine extract according to claim 9, wherein the Chinese herbal medicine extract The form is selected from the group consisting of a solution, a powder, an ointment, a film or a cream.

A Chinese herbal medicine composition having a moisturizing action, comprising the herbal extract of claim 9 or 10.

12. Use of a Chinese herbal composition according to claim 11 in cosmetics.

The use according to claim 5 or 12, wherein the cosmetic is a cosmetic skin cream, an eye cream, a facial mask, a facial cleanser, a toner or a serum.