WO2012088412A3 - Capture and enrichment of organisms and cells from liquids - Google Patents

Capture and enrichment of organisms and cells from liquids Download PDF

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Publication number
WO2012088412A3
WO2012088412A3 PCT/US2011/066838 US2011066838W WO2012088412A3 WO 2012088412 A3 WO2012088412 A3 WO 2012088412A3 US 2011066838 W US2011066838 W US 2011066838W WO 2012088412 A3 WO2012088412 A3 WO 2012088412A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
cells
sample
pellet
captured
becomes
Prior art date
Application number
PCT/US2011/066838
Other languages
French (fr)
Other versions
WO2012088412A2 (en )
Inventor
Michael Nicholson VAN HOY
Original Assignee
Becton, Dickinson And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745Assays involving non-enzymic blood coagulation factors
    • G01N2333/75Fibrin; Fibrinogen
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/948Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974Thrombin

Abstract

This invention relates to methods for collecting and processing specimens for diagnostic testing. Microorganisms and other cells are captured and enriched from liquid samples such as stomached/liquid food, urine, sputum, blood culture media, and blood, thereby enabling early detection of infections at the point of care. The method of the invention involves adding thrombin and fibrinogen to a biological sample that may contain cells including pathogens. After incubation, the sample becomes a homogenous gel. Brief agitation re-liquefies the sample, except for a very small portion that becomes a pellet which captures the cells. The pellet may then be enzymatically dissolved, resulting in a substantial percentage of the cells being captured in approximately 1/100th of the original sample, but without many of the original sample impurities.
PCT/US2011/066838 2010-12-22 2011-12-22 Capture and enrichment of organisms and cells from liquids WO2012088412A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US201061426112 true 2010-12-22 2010-12-22
US61/426,112 2010-12-22

Publications (2)

Publication Number Publication Date
WO2012088412A2 true WO2012088412A2 (en) 2012-06-28
WO2012088412A3 true true WO2012088412A3 (en) 2014-04-10

Family

ID=46314936

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/066838 WO2012088412A3 (en) 2010-12-22 2011-12-22 Capture and enrichment of organisms and cells from liquids

Country Status (1)

Country Link
WO (1) WO2012088412A3 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020197637A1 (en) * 2001-06-02 2002-12-26 Willson Richard C. Process and compositions for protection of nucleic acids
US6503731B2 (en) * 1997-09-19 2003-01-07 Hadasit Medical Research & Development Ltd. Fibrin microbeads prepared from fibrinogen, thrombin and factor XIII for binding cells
US20100143878A1 (en) * 2006-10-06 2010-06-10 Promega Corporation Methods and kits for isolating cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6503731B2 (en) * 1997-09-19 2003-01-07 Hadasit Medical Research & Development Ltd. Fibrin microbeads prepared from fibrinogen, thrombin and factor XIII for binding cells
US20020197637A1 (en) * 2001-06-02 2002-12-26 Willson Richard C. Process and compositions for protection of nucleic acids
US20100143878A1 (en) * 2006-10-06 2010-06-10 Promega Corporation Methods and kits for isolating cells

Also Published As

Publication number Publication date Type
WO2012088412A2 (en) 2012-06-28 application

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