WO2012068107A3 - Autoantibody to rna-protein complex detected by quantitative pcr - Google Patents

Autoantibody to rna-protein complex detected by quantitative pcr Download PDF

Info

Publication number
WO2012068107A3
WO2012068107A3 PCT/US2011/060789 US2011060789W WO2012068107A3 WO 2012068107 A3 WO2012068107 A3 WO 2012068107A3 US 2011060789 W US2011060789 W US 2011060789W WO 2012068107 A3 WO2012068107 A3 WO 2012068107A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
protein complex
autoantibody
protein
aggregate
Prior art date
Application number
PCT/US2011/060789
Other languages
French (fr)
Other versions
WO2012068107A2 (en
Inventor
Angela Ceribelli
Edward K. L. Chan
Minoru Satoh
Original Assignee
University Of Florida Research Foundation, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US41453710P priority Critical
Priority to US61/414,537 priority
Application filed by University Of Florida Research Foundation, Inc. filed Critical University Of Florida Research Foundation, Inc.
Publication of WO2012068107A2 publication Critical patent/WO2012068107A2/en
Publication of WO2012068107A3 publication Critical patent/WO2012068107A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The disclosure provides methods of detecting an autoantibody from a serum sample from a human or animal that is specific for an RNA:protein complex, the method comprising the steps of: (a) obtaining an immunoglobulin-RNA:protein aggregate, wherein said aggregate can comprise a target RNA:protein complex and an RNA:protein complex-specific antibody; (b) isolating the RNA from the immunoglobulin-RNA-protein aggregate; and (c) detecting the isolated RNA by quantitative reverse transcription PCR, whereby detection of the RNA can indicate the presence of the RNA:protein complex-specific antibody in a subject human or animal. One example of the method is configured for the detection of autoantibodies specifically binding to a U3 RNA: protein complex.
PCT/US2011/060789 2010-11-17 2011-11-15 Autoantibody to rna-protein complex detected by quantitative pcr WO2012068107A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US41453710P true 2010-11-17 2010-11-17
US61/414,537 2010-11-17

Publications (2)

Publication Number Publication Date
WO2012068107A2 WO2012068107A2 (en) 2012-05-24
WO2012068107A3 true WO2012068107A3 (en) 2012-08-09

Family

ID=46084604

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/060789 WO2012068107A2 (en) 2010-11-17 2011-11-15 Autoantibody to rna-protein complex detected by quantitative pcr

Country Status (1)

Country Link
WO (1) WO2012068107A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5385824A (en) * 1991-01-22 1995-01-31 Akzo N.V. Method for detection of anti-RNA-antibodies
US5541291A (en) * 1984-12-31 1996-07-30 Duke University Methods and compositions useful in the diagnosis and treatment of autoimmune diseases
US20030013134A1 (en) * 1998-02-23 2003-01-16 Mark Roth Method of diagnosing autoimmune disease
US20040180385A1 (en) * 2003-03-14 2004-09-16 Dooil Jeoung Method of diagnosing systemic lupus erythematosus
US20050227251A1 (en) * 2003-10-23 2005-10-13 Robert Darnell Method of purifying RNA binding protein-RNA complexes
US20080254461A1 (en) * 1999-12-28 2008-10-16 Ribonomics, Inc. METHODS FOR ISOLATING AND CHARACTERIZING ENDOGENOUS mRNA-PROTEIN (mRNP) COMPLEXES

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541291A (en) * 1984-12-31 1996-07-30 Duke University Methods and compositions useful in the diagnosis and treatment of autoimmune diseases
US5385824A (en) * 1991-01-22 1995-01-31 Akzo N.V. Method for detection of anti-RNA-antibodies
US20030013134A1 (en) * 1998-02-23 2003-01-16 Mark Roth Method of diagnosing autoimmune disease
US20080254461A1 (en) * 1999-12-28 2008-10-16 Ribonomics, Inc. METHODS FOR ISOLATING AND CHARACTERIZING ENDOGENOUS mRNA-PROTEIN (mRNP) COMPLEXES
US20040180385A1 (en) * 2003-03-14 2004-09-16 Dooil Jeoung Method of diagnosing systemic lupus erythematosus
US20050227251A1 (en) * 2003-10-23 2005-10-13 Robert Darnell Method of purifying RNA binding protein-RNA complexes

Also Published As

Publication number Publication date
WO2012068107A2 (en) 2012-05-24

Similar Documents

Publication Publication Date Title
AU2010229924B2 (en) Devices and methods of cell capture and analysis
MX2010000533A (en) Multiplexed analyses of test samples.
WO2005089524A3 (en) Compositions and methods for detection of single molecules
WO2012047889A3 (en) Systems and methods for automated reusable parallel biological reactions
MX350074B (en) Techniques for predicting, detecting and reducing aspecific protein interference in assays involving immunoglobulin single variable domains.
BRPI1009874A2 (en) system and method of determining analyte concentration in fluid sample and biosensor
WO2006047670A3 (en) Methods for assessing antibodies to neurodegenerative disease-associated antigens
WO2010088288A3 (en) Determination of copy number differences by amplification
EP2145180A4 (en) Comparative sequence analysis processes and systems
EA201600085A1 (en) Kit for detection of soyo synt synt0h2 event
WO2012019193A3 (en) Assay systems for genetic analysis
NZ716369A (en) Anti-ox40 antibodies and methods of using the same
WO2010027870A3 (en) Assay methods for increased throughput of samples and/or targets
WO2011022093A3 (en) Methods and devices for detecting the presence of an analyte in a sample
WO2011093602A3 (en) Centrifugal micro-fluidic device and method for detecting analytes from liquid specimen
SG185543A1 (en) Assays for the detection of genotype, mutations, and/or aneuploidy
WO2012024650A3 (en) Anti-ngf antibodies and their use
MX2012004619A (en) Proximity-mediated assays for detecting oncogenic fusion proteins.
EP1605044A4 (en) Marker for detecting mesenchymal stem cell and method of distinguishing mesenchymal stem cell using the marker
WO2011109364A3 (en) Ultra-sensitive detection of molecules or particles using beads or other capture objects
EP2534257A4 (en) Methods for identifying and isolating cells expressing a polypeptide
NZ569311A (en) Real time binding analysis of antigens on a biosensor surface
BRPI0818877A2 (en) Method and system for semi-quantitative or quantitative assay analysis
CA2749846A1 (en) Methods of determining patient response by measurement of her-3
SI2243030T2 (en) Methods for identification of an antibody or a target

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11841207

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct app. not ent. europ. phase

Ref document number: 11841207

Country of ref document: EP

Kind code of ref document: A2