WO2012064739A3 - Microbial enrichment primers - Google Patents
Microbial enrichment primers Download PDFInfo
- Publication number
- WO2012064739A3 WO2012064739A3 PCT/US2011/059783 US2011059783W WO2012064739A3 WO 2012064739 A3 WO2012064739 A3 WO 2012064739A3 US 2011059783 W US2011059783 W US 2011059783W WO 2012064739 A3 WO2012064739 A3 WO 2012064739A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- enrichment primers
- microbial enrichment
- microbial
- primer set
- primers
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
Abstract
A primer set, referred to as the Microbial Enrichment Primers (MEP) that generates cDNA libraries depleted of ribosomal and mitochondrial sequences is provided. The primer set is useful in a method that enriches for the presence of microbial nucleic acids from samples. The method enhances sensitivity of microbe detection.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US41114210P | 2010-11-08 | 2010-11-08 | |
US61/411,142 | 2010-11-08 | ||
US201061424276P | 2010-12-17 | 2010-12-17 | |
US61/424,276 | 2010-12-17 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2012064739A2 WO2012064739A2 (en) | 2012-05-18 |
WO2012064739A3 true WO2012064739A3 (en) | 2012-07-19 |
Family
ID=46051514
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2011/059783 WO2012064739A2 (en) | 2010-11-08 | 2011-11-08 | Microbial enrichment primers |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2012064739A2 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9487828B2 (en) | 2012-05-10 | 2016-11-08 | The General Hospital Corporation | Methods for determining a nucleotide sequence contiguous to a known target nucleotide sequence |
US10450597B2 (en) | 2014-01-27 | 2019-10-22 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
GB2531741A (en) * | 2014-10-28 | 2016-05-04 | Bisn Laboratory Services Ltd | Molecular and bioinformatics methods for direct sequencing |
CN112927756B (en) * | 2019-12-06 | 2023-05-30 | 深圳华大基因科技服务有限公司 | Method and device for identifying rRNA pollution source of transcriptome and method for improving rRNA pollution |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5770402A (en) * | 1995-04-05 | 1998-06-23 | Board Of Regents, The University Of Texas System | DNA encoding macrophage inflammatory protein-1γ |
US6068991A (en) * | 1997-12-16 | 2000-05-30 | Bristol-Myers Squibb Company | High expression Escherichia coli expression vector |
US20030224357A1 (en) * | 2000-06-07 | 2003-12-04 | Santalucia John | Method and system for predicting nucleic acid hybridization thermodynamics and computer-readable storage medium for use therein |
US20030229044A1 (en) * | 2002-03-29 | 2003-12-11 | Lawrence Steinman | Use of statins and other immunomodulatory agents in the treatment of autoimmune disease |
US20080187969A1 (en) * | 2005-10-27 | 2008-08-07 | Rosetta Inpharmatics Llc | Nucleic acid amplification using non-random primers |
US20100029511A1 (en) * | 2007-10-26 | 2010-02-04 | Rosetta Inpharmatics Llc | Cdna synthesis using non-random primers |
US20100120022A1 (en) * | 2004-01-27 | 2010-05-13 | Michal Ayalon-Soffer | Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis |
-
2011
- 2011-11-08 WO PCT/US2011/059783 patent/WO2012064739A2/en active Application Filing
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5770402A (en) * | 1995-04-05 | 1998-06-23 | Board Of Regents, The University Of Texas System | DNA encoding macrophage inflammatory protein-1γ |
US6068991A (en) * | 1997-12-16 | 2000-05-30 | Bristol-Myers Squibb Company | High expression Escherichia coli expression vector |
US20030224357A1 (en) * | 2000-06-07 | 2003-12-04 | Santalucia John | Method and system for predicting nucleic acid hybridization thermodynamics and computer-readable storage medium for use therein |
US20030229044A1 (en) * | 2002-03-29 | 2003-12-11 | Lawrence Steinman | Use of statins and other immunomodulatory agents in the treatment of autoimmune disease |
US20100120022A1 (en) * | 2004-01-27 | 2010-05-13 | Michal Ayalon-Soffer | Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis |
US20080187969A1 (en) * | 2005-10-27 | 2008-08-07 | Rosetta Inpharmatics Llc | Nucleic acid amplification using non-random primers |
US20100029511A1 (en) * | 2007-10-26 | 2010-02-04 | Rosetta Inpharmatics Llc | Cdna synthesis using non-random primers |
Also Published As
Publication number | Publication date |
---|---|
WO2012064739A2 (en) | 2012-05-18 |
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