WO2012064739A3 - Microbial enrichment primers - Google Patents

Microbial enrichment primers Download PDF

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Publication number
WO2012064739A3
WO2012064739A3 PCT/US2011/059783 US2011059783W WO2012064739A3 WO 2012064739 A3 WO2012064739 A3 WO 2012064739A3 US 2011059783 W US2011059783 W US 2011059783W WO 2012064739 A3 WO2012064739 A3 WO 2012064739A3
Authority
WO
WIPO (PCT)
Prior art keywords
enrichment primers
microbial enrichment
microbial
primer set
primers
Prior art date
Application number
PCT/US2011/059783
Other languages
French (fr)
Other versions
WO2012064739A2 (en
Inventor
Lan QUAN
Alexander Solovyov
W. Ian Lipkin
Original Assignee
The Trustees Of Columbia University In The City Of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Trustees Of Columbia University In The City Of New York filed Critical The Trustees Of Columbia University In The City Of New York
Publication of WO2012064739A2 publication Critical patent/WO2012064739A2/en
Publication of WO2012064739A3 publication Critical patent/WO2012064739A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection

Abstract

A primer set, referred to as the Microbial Enrichment Primers (MEP) that generates cDNA libraries depleted of ribosomal and mitochondrial sequences is provided. The primer set is useful in a method that enriches for the presence of microbial nucleic acids from samples. The method enhances sensitivity of microbe detection.
PCT/US2011/059783 2010-11-08 2011-11-08 Microbial enrichment primers WO2012064739A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41114210P 2010-11-08 2010-11-08
US61/411,142 2010-11-08
US201061424276P 2010-12-17 2010-12-17
US61/424,276 2010-12-17

Publications (2)

Publication Number Publication Date
WO2012064739A2 WO2012064739A2 (en) 2012-05-18
WO2012064739A3 true WO2012064739A3 (en) 2012-07-19

Family

ID=46051514

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/059783 WO2012064739A2 (en) 2010-11-08 2011-11-08 Microbial enrichment primers

Country Status (1)

Country Link
WO (1) WO2012064739A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9487828B2 (en) 2012-05-10 2016-11-08 The General Hospital Corporation Methods for determining a nucleotide sequence contiguous to a known target nucleotide sequence
US10450597B2 (en) 2014-01-27 2019-10-22 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
GB2531741A (en) * 2014-10-28 2016-05-04 Bisn Laboratory Services Ltd Molecular and bioinformatics methods for direct sequencing
CN112927756B (en) * 2019-12-06 2023-05-30 深圳华大基因科技服务有限公司 Method and device for identifying rRNA pollution source of transcriptome and method for improving rRNA pollution

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770402A (en) * 1995-04-05 1998-06-23 Board Of Regents, The University Of Texas System DNA encoding macrophage inflammatory protein-1γ
US6068991A (en) * 1997-12-16 2000-05-30 Bristol-Myers Squibb Company High expression Escherichia coli expression vector
US20030224357A1 (en) * 2000-06-07 2003-12-04 Santalucia John Method and system for predicting nucleic acid hybridization thermodynamics and computer-readable storage medium for use therein
US20030229044A1 (en) * 2002-03-29 2003-12-11 Lawrence Steinman Use of statins and other immunomodulatory agents in the treatment of autoimmune disease
US20080187969A1 (en) * 2005-10-27 2008-08-07 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
US20100029511A1 (en) * 2007-10-26 2010-02-04 Rosetta Inpharmatics Llc Cdna synthesis using non-random primers
US20100120022A1 (en) * 2004-01-27 2010-05-13 Michal Ayalon-Soffer Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770402A (en) * 1995-04-05 1998-06-23 Board Of Regents, The University Of Texas System DNA encoding macrophage inflammatory protein-1γ
US6068991A (en) * 1997-12-16 2000-05-30 Bristol-Myers Squibb Company High expression Escherichia coli expression vector
US20030224357A1 (en) * 2000-06-07 2003-12-04 Santalucia John Method and system for predicting nucleic acid hybridization thermodynamics and computer-readable storage medium for use therein
US20030229044A1 (en) * 2002-03-29 2003-12-11 Lawrence Steinman Use of statins and other immunomodulatory agents in the treatment of autoimmune disease
US20100120022A1 (en) * 2004-01-27 2010-05-13 Michal Ayalon-Soffer Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
US20080187969A1 (en) * 2005-10-27 2008-08-07 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
US20100029511A1 (en) * 2007-10-26 2010-02-04 Rosetta Inpharmatics Llc Cdna synthesis using non-random primers

Also Published As

Publication number Publication date
WO2012064739A2 (en) 2012-05-18

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