WO2011156784A1 - Compositions and methods of treating no-option critical limb ischemia (cli) - Google Patents
Compositions and methods of treating no-option critical limb ischemia (cli) Download PDFInfo
- Publication number
- WO2011156784A1 WO2011156784A1 PCT/US2011/040087 US2011040087W WO2011156784A1 WO 2011156784 A1 WO2011156784 A1 WO 2011156784A1 US 2011040087 W US2011040087 W US 2011040087W WO 2011156784 A1 WO2011156784 A1 WO 2011156784A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cells
- composition
- subject
- amputation
- cli
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 197
- 208000032064 Chronic Limb-Threatening Ischemia Diseases 0.000 title claims abstract description 148
- 206010034576 Peripheral ischaemia Diseases 0.000 title claims abstract description 148
- 238000000034 method Methods 0.000 title claims abstract description 138
- 238000002266 amputation Methods 0.000 claims abstract description 106
- 206010052428 Wound Diseases 0.000 claims abstract description 88
- 208000027418 Wounds and injury Diseases 0.000 claims abstract description 88
- 230000001965 increasing effect Effects 0.000 claims abstract description 81
- 230000003247 decreasing effect Effects 0.000 claims abstract description 72
- 230000004083 survival effect Effects 0.000 claims abstract description 55
- 230000000250 revascularization Effects 0.000 claims abstract description 53
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 claims abstract description 50
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 claims abstract description 50
- 206010053648 Vascular occlusion Diseases 0.000 claims abstract description 46
- 208000021331 vascular occlusion disease Diseases 0.000 claims abstract description 46
- 238000010561 standard procedure Methods 0.000 claims abstract description 41
- 230000017423 tissue regeneration Effects 0.000 claims abstract description 36
- 206010017711 Gangrene Diseases 0.000 claims abstract description 35
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 claims abstract description 33
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 claims abstract description 33
- 238000003306 harvesting Methods 0.000 claims abstract description 33
- 230000029663 wound healing Effects 0.000 claims abstract description 33
- 230000034994 death Effects 0.000 claims abstract description 30
- 231100000517 death Toxicity 0.000 claims abstract description 30
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 29
- 230000035899 viability Effects 0.000 claims abstract description 29
- 238000011109 contamination Methods 0.000 claims abstract description 27
- 230000003394 haemopoietic effect Effects 0.000 claims abstract description 25
- 239000002158 endotoxin Substances 0.000 claims abstract description 24
- 241000204031 Mycoplasma Species 0.000 claims abstract description 22
- 230000000813 microbial effect Effects 0.000 claims abstract description 22
- 230000003511 endothelial effect Effects 0.000 claims abstract description 21
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 19
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 18
- 210000004027 cell Anatomy 0.000 claims description 306
- 238000011282 treatment Methods 0.000 claims description 82
- 210000003414 extremity Anatomy 0.000 claims description 65
- 210000001519 tissue Anatomy 0.000 claims description 44
- 210000001185 bone marrow Anatomy 0.000 claims description 38
- 238000005259 measurement Methods 0.000 claims description 38
- 230000036407 pain Effects 0.000 claims description 38
- 210000005087 mononuclear cell Anatomy 0.000 claims description 27
- 210000002414 leg Anatomy 0.000 claims description 21
- 230000002265 prevention Effects 0.000 claims description 17
- 239000003814 drug Substances 0.000 claims description 16
- 210000002966 serum Anatomy 0.000 claims description 15
- 206010061218 Inflammation Diseases 0.000 claims description 14
- 230000004054 inflammatory process Effects 0.000 claims description 13
- 238000004393 prognosis Methods 0.000 claims description 12
- 206010028851 Necrosis Diseases 0.000 claims description 11
- 230000017074 necrotic cell death Effects 0.000 claims description 11
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 claims description 10
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 claims description 10
- 230000033115 angiogenesis Effects 0.000 claims description 10
- 230000003111 delayed effect Effects 0.000 claims description 10
- 102000008186 Collagen Human genes 0.000 claims description 9
- 108010035532 Collagen Proteins 0.000 claims description 9
- 208000025865 Ulcer Diseases 0.000 claims description 9
- 229920001436 collagen Polymers 0.000 claims description 9
- 230000008602 contraction Effects 0.000 claims description 9
- 239000008151 electrolyte solution Substances 0.000 claims description 9
- 239000012091 fetal bovine serum Substances 0.000 claims description 9
- 230000037313 granulation tissue formation Effects 0.000 claims description 9
- 238000007634 remodeling Methods 0.000 claims description 9
- 230000035488 systolic blood pressure Effects 0.000 claims description 9
- 230000036269 ulceration Effects 0.000 claims description 9
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 claims description 8
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 claims description 8
- 230000001605 fetal effect Effects 0.000 claims description 8
- 230000000302 ischemic effect Effects 0.000 claims description 8
- 210000003141 lower extremity Anatomy 0.000 claims description 8
- 238000011084 recovery Methods 0.000 claims description 8
- 210000004233 talus Anatomy 0.000 claims description 8
- 238000010255 intramuscular injection Methods 0.000 claims description 7
- 239000007927 intramuscular injection Substances 0.000 claims description 7
- 210000005259 peripheral blood Anatomy 0.000 claims description 7
- 239000011886 peripheral blood Substances 0.000 claims description 7
- 238000009556 duplex ultrasonography Methods 0.000 claims description 6
- 210000004700 fetal blood Anatomy 0.000 claims description 6
- 210000002683 foot Anatomy 0.000 claims description 6
- 238000003384 imaging method Methods 0.000 claims description 6
- 210000004185 liver Anatomy 0.000 claims description 6
- 229940124583 pain medication Drugs 0.000 claims description 6
- 210000003423 ankle Anatomy 0.000 claims description 4
- 238000002355 open surgical procedure Methods 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 101000851018 Homo sapiens Vascular endothelial growth factor receptor 1 Proteins 0.000 claims description 2
- 102100033178 Vascular endothelial growth factor receptor 1 Human genes 0.000 claims description 2
- 238000004458 analytical method Methods 0.000 description 48
- 239000000243 solution Substances 0.000 description 27
- 206010066901 Treatment failure Diseases 0.000 description 22
- 239000000306 component Substances 0.000 description 22
- 210000000130 stem cell Anatomy 0.000 description 22
- 238000002347 injection Methods 0.000 description 21
- 239000007924 injection Substances 0.000 description 21
- 230000006870 function Effects 0.000 description 17
- 239000002609 medium Substances 0.000 description 17
- 230000008569 process Effects 0.000 description 16
- 238000002560 therapeutic procedure Methods 0.000 description 16
- 238000000926 separation method Methods 0.000 description 15
- 230000008901 benefit Effects 0.000 description 14
- 239000000047 product Substances 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 13
- 206010012601 diabetes mellitus Diseases 0.000 description 12
- 239000007788 liquid Substances 0.000 description 12
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 10
- 230000009467 reduction Effects 0.000 description 10
- 230000002411 adverse Effects 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 210000002304 esc Anatomy 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 201000010099 disease Diseases 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 8
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 8
- 238000001325 log-rank test Methods 0.000 description 8
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 8
- 210000003371 toe Anatomy 0.000 description 8
- 102100035248 Alpha-(1,3)-fucosyltransferase 4 Human genes 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 238000000729 Fisher's exact test Methods 0.000 description 7
- 101001022185 Homo sapiens Alpha-(1,3)-fucosyltransferase 4 Proteins 0.000 description 7
- 102100038021 Steryl-sulfatase Human genes 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- 239000008280 blood Substances 0.000 description 7
- 230000007423 decrease Effects 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- 102000006639 indoleamine 2,3-dioxygenase Human genes 0.000 description 7
- 108020004201 indoleamine 2,3-dioxygenase Proteins 0.000 description 7
- 239000012071 phase Substances 0.000 description 7
- 210000002798 bone marrow cell Anatomy 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 6
- 238000002659 cell therapy Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 239000008177 pharmaceutical agent Substances 0.000 description 6
- 239000000902 placebo Substances 0.000 description 6
- 229940068196 placebo Drugs 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 108010074708 B7-H1 Antigen Proteins 0.000 description 5
- 108091006905 Human Serum Albumin Proteins 0.000 description 5
- 102000008100 Human Serum Albumin Human genes 0.000 description 5
- 239000007760 Iscove's Modified Dulbecco's Medium Substances 0.000 description 5
- 208000008589 Obesity Diseases 0.000 description 5
- 102100024216 Programmed cell death 1 ligand 1 Human genes 0.000 description 5
- 235000012000 cholesterol Nutrition 0.000 description 5
- 239000002131 composite material Substances 0.000 description 5
- 208000019622 heart disease Diseases 0.000 description 5
- 230000006872 improvement Effects 0.000 description 5
- 230000002757 inflammatory effect Effects 0.000 description 5
- 150000002632 lipids Chemical class 0.000 description 5
- 210000002540 macrophage Anatomy 0.000 description 5
- 210000003205 muscle Anatomy 0.000 description 5
- 230000001172 regenerating effect Effects 0.000 description 5
- 239000007858 starting material Substances 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 206010003210 Arteriosclerosis Diseases 0.000 description 4
- 201000001320 Atherosclerosis Diseases 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 108010074328 Interferon-gamma Proteins 0.000 description 4
- 102000003814 Interleukin-10 Human genes 0.000 description 4
- 108090000174 Interleukin-10 Proteins 0.000 description 4
- 230000003276 anti-hypertensive effect Effects 0.000 description 4
- 230000003110 anti-inflammatory effect Effects 0.000 description 4
- 208000011775 arteriosclerosis disease Diseases 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 244000309466 calf Species 0.000 description 4
- 239000002458 cell surface marker Substances 0.000 description 4
- 230000003833 cell viability Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 4
- 239000003792 electrolyte Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 230000035876 healing Effects 0.000 description 4
- 229960000890 hydrocortisone Drugs 0.000 description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 210000001616 monocyte Anatomy 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 230000010412 perfusion Effects 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 238000001356 surgical procedure Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 206010007882 Cellulitis Diseases 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 102000013462 Interleukin-12 Human genes 0.000 description 3
- 108010065805 Interleukin-12 Proteins 0.000 description 3
- 102000004889 Interleukin-6 Human genes 0.000 description 3
- 108090001005 Interleukin-6 Proteins 0.000 description 3
- 210000001744 T-lymphocyte Anatomy 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 230000008827 biological function Effects 0.000 description 3
- 230000017531 blood circulation Effects 0.000 description 3
- 230000036772 blood pressure Effects 0.000 description 3
- 238000009583 bone marrow aspiration Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000001804 debridement Methods 0.000 description 3
- 210000001671 embryonic stem cell Anatomy 0.000 description 3
- 230000007717 exclusion Effects 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 229940076144 interleukin-10 Drugs 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 235000020824 obesity Nutrition 0.000 description 3
- 239000008194 pharmaceutical composition Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 230000003442 weekly effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101710155857 C-C motif chemokine 2 Proteins 0.000 description 2
- 102000000018 Chemokine CCL2 Human genes 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 101000884279 Homo sapiens CD276 antigen Proteins 0.000 description 2
- 101000743488 Homo sapiens V-set and immunoglobulin domain-containing protein 4 Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 2
- 108090001061 Insulin Proteins 0.000 description 2
- 102100037850 Interferon gamma Human genes 0.000 description 2
- 102000008070 Interferon-gamma Human genes 0.000 description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
- 229930182816 L-glutamine Natural products 0.000 description 2
- 206010024774 Localised infection Diseases 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 206010040943 Skin Ulcer Diseases 0.000 description 2
- 230000006044 T cell activation Effects 0.000 description 2
- 102100038296 V-set and immunoglobulin domain-containing protein 4 Human genes 0.000 description 2
- 108010059993 Vancomycin Proteins 0.000 description 2
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 2
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 2
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000002870 angiogenesis inducing agent Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000702 anti-platelet effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 238000013176 antiplatelet therapy Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 210000001367 artery Anatomy 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 238000011072 cell harvest Methods 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 108091092356 cellular DNA Proteins 0.000 description 2
- 208000012696 congenital leptin deficiency Diseases 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 230000001934 delay Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 108010067396 dornase alfa Proteins 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- 229960003130 interferon gamma Drugs 0.000 description 2
- 229940117681 interleukin-12 Drugs 0.000 description 2
- 229940100601 interleukin-6 Drugs 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 210000003127 knee Anatomy 0.000 description 2
- 238000011542 limb amputation Methods 0.000 description 2
- 229920006008 lipopolysaccharide Polymers 0.000 description 2
- 238000007726 management method Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 208000001022 morbid obesity Diseases 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000001558 permutation test Methods 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 230000005195 poor health Effects 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 230000001023 pro-angiogenic effect Effects 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 108091006091 regulatory enzymes Proteins 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000011477 surgical intervention Methods 0.000 description 2
- 230000009885 systemic effect Effects 0.000 description 2
- 230000000699 topical effect Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 2
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 2
- 210000005166 vasculature Anatomy 0.000 description 2
- 208000016261 weight loss Diseases 0.000 description 2
- 230000004580 weight loss Effects 0.000 description 2
- LAQPKDLYOBZWBT-NYLDSJSYSA-N (2s,4s,5r,6r)-5-acetamido-2-{[(2s,3r,4s,5s,6r)-2-{[(2r,3r,4r,5r)-5-acetamido-1,2-dihydroxy-6-oxo-4-{[(2s,3s,4r,5s,6s)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy}hexan-3-yl]oxy}-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy}-4-hydroxy-6-[(1r,2r)-1,2,3-trihydrox Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@H]([C@@H](NC(C)=O)C=O)[C@@H]([C@H](O)CO)O[C@H]1[C@H](O)[C@@H](O[C@]2(O[C@H]([C@H](NC(C)=O)[C@@H](O)C2)[C@H](O)[C@H](O)CO)C(O)=O)[C@@H](O)[C@@H](CO)O1 LAQPKDLYOBZWBT-NYLDSJSYSA-N 0.000 description 1
- FUFLCEKSBBHCMO-UHFFFAOYSA-N 11-dehydrocorticosterone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 FUFLCEKSBBHCMO-UHFFFAOYSA-N 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 description 1
- 208000020446 Cardiac disease Diseases 0.000 description 1
- 206010007559 Cardiac failure congestive Diseases 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- MFYSYFVPBJMHGN-ZPOLXVRWSA-N Cortisone Chemical compound O=C1CC[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 MFYSYFVPBJMHGN-ZPOLXVRWSA-N 0.000 description 1
- MFYSYFVPBJMHGN-UHFFFAOYSA-N Cortisone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 MFYSYFVPBJMHGN-UHFFFAOYSA-N 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000009329 Graft vs Host Disease Diseases 0.000 description 1
- 229940121710 HMGCoA reductase inhibitor Drugs 0.000 description 1
- 206010020100 Hip fracture Diseases 0.000 description 1
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 description 1
- 101000994378 Homo sapiens Integrin alpha-3 Proteins 0.000 description 1
- 101001057504 Homo sapiens Interferon-stimulated gene 20 kDa protein Proteins 0.000 description 1
- 101001076407 Homo sapiens Interleukin-1 receptor antagonist protein Proteins 0.000 description 1
- 101001055144 Homo sapiens Interleukin-2 receptor subunit alpha Proteins 0.000 description 1
- 206010021138 Hypovolaemic shock Diseases 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- 102100032819 Integrin alpha-3 Human genes 0.000 description 1
- 229940119178 Interleukin 1 receptor antagonist Drugs 0.000 description 1
- 102000051628 Interleukin-1 receptor antagonist Human genes 0.000 description 1
- 102100026878 Interleukin-2 receptor subunit alpha Human genes 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- FQISKWAFAHGMGT-SGJOWKDISA-M Methylprednisolone sodium succinate Chemical compound [Na+].C([C@@]12C)=CC(=O)C=C1[C@@H](C)C[C@@H]1[C@@H]2[C@@H](O)C[C@]2(C)[C@@](O)(C(=O)COC(=O)CCC([O-])=O)CC[C@H]21 FQISKWAFAHGMGT-SGJOWKDISA-M 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010029113 Neovascularisation Diseases 0.000 description 1
- 208000031481 Pathologic Constriction Diseases 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 208000006735 Periostitis Diseases 0.000 description 1
- 208000005764 Peripheral Arterial Disease Diseases 0.000 description 1
- 208000030831 Peripheral arterial occlusive disease Diseases 0.000 description 1
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 206010061926 Purulence Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 206010062237 Renal impairment Diseases 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 108091008605 VEGF receptors Proteins 0.000 description 1
- 108010053096 Vascular Endothelial Growth Factor Receptor-1 Proteins 0.000 description 1
- 102000016548 Vascular Endothelial Growth Factor Receptor-1 Human genes 0.000 description 1
- 102100033177 Vascular endothelial growth factor receptor 2 Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 206010058041 Wound sepsis Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- -1 and the like Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000008365 aqueous carrier Substances 0.000 description 1
- 230000008321 arterial blood flow Effects 0.000 description 1
- 238000012865 aseptic processing Methods 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 238000011130 autologous cell therapy Methods 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000002771 cell marker Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000003021 clonogenic effect Effects 0.000 description 1
- 239000012468 concentrated sample Substances 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 229960004544 cortisone Drugs 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000011461 current therapy Methods 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 238000002674 endoscopic surgery Methods 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001317 epifluorescence microscopy Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 239000012997 ficoll-paque Substances 0.000 description 1
- 239000013020 final formulation Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000002695 general anesthesia Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 208000005017 glioblastoma Diseases 0.000 description 1
- 230000024924 glomerular filtration Effects 0.000 description 1
- 208000024908 graft versus host disease Diseases 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 210000001255 hallux Anatomy 0.000 description 1
- 210000004247 hand Anatomy 0.000 description 1
- 238000012787 harvest procedure Methods 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- VWQWXZAWFPZJDA-CGVGKPPMSA-N hydrocortisone succinate Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)COC(=O)CCC(O)=O)[C@@H]4[C@@H]3CCC2=C1 VWQWXZAWFPZJDA-CGVGKPPMSA-N 0.000 description 1
- 230000002631 hypothermal effect Effects 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 229940124589 immunosuppressive drug Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000003407 interleukin 1 receptor blocking agent Substances 0.000 description 1
- 238000007913 intrathecal administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 230000003447 ipsilateral effect Effects 0.000 description 1
- 208000028867 ischemia Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 238000002690 local anesthesia Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229940097364 magnesium acetate tetrahydrate Drugs 0.000 description 1
- XKPKPGCRSHFTKM-UHFFFAOYSA-L magnesium;diacetate;tetrahydrate Chemical compound O.O.O.O.[Mg+2].CC([O-])=O.CC([O-])=O XKPKPGCRSHFTKM-UHFFFAOYSA-L 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012569 microbial contaminant Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 238000002324 minimally invasive surgery Methods 0.000 description 1
- 239000007758 minimum essential medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000001178 neural stem cell Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 210000003460 periosteum Anatomy 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 238000009521 phase II clinical trial Methods 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 238000000554 physical therapy Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 238000012794 pre-harvesting Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 229940107568 pulmozyme Drugs 0.000 description 1
- 230000000541 pulsatile effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 210000003289 regulatory T cell Anatomy 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 231100000279 safety data Toxicity 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 206010040560 shock Diseases 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 231100000019 skin ulcer Toxicity 0.000 description 1
- 229940088542 solu-cortef Drugs 0.000 description 1
- 229940087854 solu-medrol Drugs 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000011272 standard treatment Methods 0.000 description 1
- 238000010972 statistical evaluation Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000036262 stenosis Effects 0.000 description 1
- 208000037804 stenosis Diseases 0.000 description 1
- 239000008174 sterile solution Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 210000000242 supportive cell Anatomy 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- CWERGRDVMFNCDR-UHFFFAOYSA-M thioglycolate(1-) Chemical compound [O-]C(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-M 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 239000001974 tryptic soy broth Substances 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 229940072335 vancocin Drugs 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
Definitions
- the present invention relates to compositions of mixed cell populations, their subsequent use in vivo for tissue repair and processes, and, in particular, to the treatment of critical limb ischemia (CLI) for those patients and subjects who present a vascular occlusion that cannot be resolved by using a standard method revascularization.
- CLI critical limb ischemia
- Regenerative medicine harnesses, in a clinically targeted manner, the ability of regenerative cells, e.g., stem cells and/or progenitor cells (i.e., the unspecialized master cells of the body), to renew themselves indefinitely and develop into mature specialized cells.
- Stem cells are found in embryos during early stages of development, in fetal tissue and in some adult organs and tissue.
- Embryonic stem cells hereinafter referred to as "ESCs" are known to become many if not all of the cell and tissue types of the body. ESCs not only contain all the genetic information of the individual but also contain the nascent capacity to become any of the 200+ cells and tissues of the body. Thus, these cells have tremendous potential for regenerative medicine.
- ESCs can be grown into specific tissues such as heart, lung or kidney which could then be used to repair damaged and diseased organs.
- ESC derived tissues have clinical limitations. Since ESCs are necessarily derived from another individual, i.e., an embryo, there is a risk that the recipient's immune system will reject the new biological material.
- immunosuppressive drugs to prevent such rejection are available, such drugs are also known to block desirable immune responses such as those against bacterial infections and viruses.
- ASCs Adult stem cells
- ESCs i.e., embryos
- ASCs represent an alternative to the use of ESCs.
- ASCs reside quietly in many non-embryonic tissues, presumably waiting to respond to trauma or other destructive disease processes so that they can heal the injured tissue.
- emerging scientific evidence indicates that each individual carries a pool of ASCs that may share with ESCs the ability to become many if not all types of cells and tissues.
- ASCs like ESCs, have tremendous potential for clinical applications of regenerative medicine.
- ASC populations have been shown to be present in one or more of bone marrow, skin, muscle, liver and brain.
- the frequency of ASCs in these tissues is low.
- mesenchymal stem cell frequency in bone marrow is estimated at between 1 in 100,000 and 1 in 1,000,000 nucleated cells
- any proposed clinical application of ASCs from such tissues requires increasing cell number, purity, and maturity by processes of cell purification and cell culture.
- cell culture steps may provide increased cell number, purity, and maturity, they do so at a cost.
- This cost can include one or more of the following technical difficulties: loss of cell function due to cell aging, loss of potentially useful cell populations, delays in potential application of cells to patients, increased monetary cost, increased risk of contamination of cells with environmental microorganisms during culture, and the need for further post-culture processing to deplete culture materials contained with the harvested cells.
- the invention provides a method of treating critical limb ischemia (CLI) in a subject, wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination, thereby improving or preventing the clinical consequence of critical limb ischemia (CLI).
- the isolated cell composition for tissue repair is also referred to herein as the tissue repair cell (TRC) composition.
- the standard method of revascularization is an open surgical procedure or a percutaneous endovascular procedure. Furthermore, the presence of a vascular occlusion that cannot be resolved by using a standard method of revascularization may be determined by physical examination, angiographic imaging, color flow duplex ultrasound, or any combination thereof.
- the subject may present a vascular occlusion in a lower extremity.
- the subject may present recurring ischemic rest pain for at least 2 weeks, ulceration, or gangrene with absent pulses in an extremity.
- the subject may further present recurring ischemic rest pain for at least 2 weeks, ulceration, or gangrene in the foot or toe with absent pedal pulses, and with either a toe systolic pressure of equal to or less than 50 mm Hg or ankle systolic pressure of equal to or less than 70 mm Hg.
- Exemplary clinical consequences of no-option critical limb ischemia include, but are not limited to, increased rest pain, decreased mobility of a limb (arm or leg), ulceration, increased wound size (doubling of wound size), decreased or impaired wound healing, de novo gangrene, decreased or absent pulse at extremity, tissue loss (tissue necrosis), amputation (for instance, of a digit, such as a finger or toe, which would not constitute a major amputation), major amputation (defined as, for example, an amputation at or above the talus on the leg), or death.
- Decreased function of an affected limb includes, but is not limited to, decreased range of motion, decreased strength, or decreased endurance for physical exertion of the limb.
- the limb is a leg and a decreased function of an affected limb includes decreased walking distance or decreased walking time.
- vascular occlusion that cannot be resolved by using a standard method of revascularization achieves a clinical goal.
- exemplary clinical goals include, but are not limited to, decreased pain, increased function of an affected limb, decreased wound size, increased wound healing, delay or prevention of de novo gangrene, delay or prevention of amputation, or increased survival.
- decreased pain is determined by comparing a demand from the subject for administration of a pain medicine or a dosage of a pain medication from a time period prior to administration of the composition to a demand from the subject for administration of a pain medicine or a dosage of a pain medication from a time point following administration of the composition, wherein a decreased demand or a decreased dosage indicates that the treatment decreased the pain of the subject following administration of the composition.
- increased function of an affected limb is determined by comparing a range of motion, a strength, or an endurance measurement for physical exertion of the limb from a time period prior to administration of the composition to a range of motion, a strength, or an endurance measurement for physical exertion of the limb from a time point following administration of the composition, wherein an increased range of motion, increased strength, or increased endurance measurement indicates that the treatment increased the function of the affected limb of the subject following administration of the composition.
- decreased wound size is determined by comparing an area, circumference, or depth measurement of a wound from a time period prior to administration of the composition to an area, circumference, or depth measurement of a wound from a time point following administration of the composition, wherein a decreased area, circumference, or depth measurement indicates that the treatment decreased size of a wound following administration of the composition.
- increased wound healing is determined by comparing a measurement of active inflammation, angiogenesis, collagen disposition, fibroplasia, granulation tissue formation, epithelialization, contraction, or remodeling of a wound from a time period prior to administration of the composition to a measurement of active inflammation, angiogenesis, collagen disposition, fibroplasias, granulation tissue formation, epithelialization, contraction, or remodeling of a wound from a time point following administration of the composition, wherein an increased measurement of active inflammation, angiogenesis, collagen disposition, fibroplasia, granulation tissue formation, epithelialization, contraction, or remodeling indicates that the treatment increased wound healing following administration of the composition.
- delay or prevention of de novo gangrene is determined by comparing a measurement of tissue necrosis from a time period prior to administration of the composition to a measurement of tissue necrosis from a time point following administration of the composition, wherein an identical or decreased
- tissue necrosis indicates that the treatment delayed or prevented the formation of de novo gangrene following administration of the composition.
- delay or prevention of amputation is determined by comparing the prognosis for amputation in the subject from a time period prior to administration of the composition to the prognosis for either amputation in the subject following administration of the composition, wherein an increase in the time required until amputation or a cancellation of the amputation procedure due to recovery indicates that the treatment delayed or prevented the amputation of the affected limb, respectively.
- increased survival is determined by comparing the prognosis for survival in the subject from a time period prior to administration of the composition to the prognosis for survival in the subject following administration of the composition, wherein an increase in predicted survival time indicates that the treatment increased survival of the subject following administration of the composition.
- composition may be administered by intramuscular injection at one or more sites.
- the composition is injected at 20 sites.
- the cells of the composition are derived from mononuclear cells. These mononuclear cells are derived from bone marrow, peripheral blood, umbilical cord blood or fetal liver.
- cells of the composition are in a pharmaceutical-grade electrolyte solution suitable for human administration.
- the composition is substantially free of horse serum and/or fetal bovine serum.
- the CD90 + cells of the composition co- express CD 15.
- the CD45 + cells of the composition are CD14 + , CD34 + or VEGFRl + .
- composition is 35 million to 300 million. In a preferred embodiment, the composition contains an average of between 90-180 x 10 6 viable cells. Moreover, the cells may be in a volume less than 15 milliliters, 10 milliliters, or 7.5 milliliters.
- the invention also provides a method of increasing amputation-free survival in a subject diagnosed with critical limb ischemia (CLI), wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination.
- CLI critical limb ischemia
- the amputation- free survival is increased in the treated subject when compared to an untreated subject, wherein the untreated subject is also diagnosed with critical limb ischemia (CLI) and also presents a vascular occlusion that cannot be resolved by using a standard method of revascularization.
- Amputation-free survival is defined as the time of administration of the composition until an amputation is performed, the subject dies, or the combination occurs.
- the invention provides a method of preventing major amputation in a subject diagnosed with critical limb ischemia (CLI), wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 ⁇ g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination.
- CLI critical limb ischemia
- the vascular occlusion occurs in a leg.
- the major amputation is an amputation at or above the talus on the leg.
- a major amputation is prevented from the time of administration of the composition until 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, or 25 years after administration.
- a major amputation is prevented because the extremity is revascularized, as confirm, for instance, by physical examination, angiographic imaging, color flow duplex ultrasound, or any combination thereof. A subject who experiences revascularization in combination with function of the extremity will avoid major amputation indefinitely, and, therefore, the major amputation has been prevented.
- the invention provides a method of delaying the onset of de novo gangrene, tissue loss, amputation, or death in a subject diagnosed with critical limb ischemia (CLI), wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination.
- CLI critical limb ischemia
- the onset of de novo gangrene, tissue loss, amputation, or death is delayed in the treated subject when compared to an untreated subject, wherein the untreated subject is also diagnosed with critical limb ischemia (CLI) and also presents a vascular occlusion that cannot be resolved by using a standard method of revascularization.
- amputation includes minor and major amputation.
- a subject with no-option CLI who also has an underlying medical condition like morbid obesity, advanced diabetes, or advanced age (with poor general health), may not be able to avoid the more severe consequences of no-option CLI forever, however, they may benefit from these methods by delaying the onset of these events for a sufficient time to experience a significant increased in quality of life. Furthermore, an elderly patient may benefit by avoiding amputation until morbidity arises from age rather than no-option CLI, thereby, benefitting by an increased quality of life for the interim.
- the concept of "treating" no-option CLI includes improving mobility, decreasing pain, improving wound healing, decreasing wound size, and delaying tissue loss, amputation, and death.
- the treatment for no-option CLI could be a cure in an otherwise healthy individual
- the measure of success for treating a subject with no-option CLI in the average subject includes ameliorating an existing symptom or delaying the onset of a worse symptom.
- the invention provides a method of increasing survival probability in a subject diagnosed with critical limb ischemia (CLI), wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 ⁇ g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination.
- CLI critical limb ischemia
- the survival probability is increased in the treated subject when compared to an untreated subject, wherein the untreated subject is also diagnosed with critical limb ischemia (CLI) and also presents a vascular occlusion that cannot be resolved by using a standard method of revascularization. Survival probability is another method of expressing the time to treatment failure, or the likelihood that the treatment will be successful.
- CLI critical limb ischemia
- the composition is administered to a subject who presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, in combination with another therapy. For instance, if the subject suffers from an underlying atherosclerosis in the limb undergoing treatment, or in another part of his or her body, the composition is administered in combination with a pharmaceutical agent.
- Contemplated pharmaceutical agents reduce lipids (lipid or cholesterol reduction therapy), reduce platelet aggregation or platelet attachment to the walls of the vasculature (anti-platelet therapy), or reduce blood pressure (anti-hypertensive therapy).
- the subject of the present methods may have a wound associated with no-option CLI on the treated limb, or on another part of his or her body.
- the composition is administered in combination with topical or systemic wound care.
- wound care includes, but is not limited to, pharmaceutical agents to decrease infection (like antibiotics), decrease inflammation, promote healing (antioxidants), and promote vascularization (pro-angiogenic factors); matrices or scaffolds to provide a substrate upon which to grow tissue for the wound; and surgical intervention to removal of dead, damaged, or infected tissue (debridement).
- the methods provided herein may also be used in combination with treatments for obesity (for instance, drugs including olitstat and the non-prescription version, alii), heart disease (for instance, drugs used to combat high cholesterol or high blood pressure), and diabetes (for example, insulin for type I and weight-loss therapy for type II).
- obesity for instance, drugs including olitstat and the non-prescription version, alii
- heart disease for instance, drugs used to combat high cholesterol or high blood pressure
- diabetes for example, insulin for type I and weight-loss therapy for type II.
- the invention provides an isolated cell composition for tissue repair containing a mixed population of cells.
- the cells are in a pharmaceutical-grade electrolyte solution suitable for human administration.
- the cells are derived from mononuclear cells.
- the cells are derived from bone marrow, peripheral blood, umbilical cord blood or fetal liver.
- the cells are of hematopoietic, mesenchymal and endothelial lineage.
- the viability of cells is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or greater.
- the total number of viable cells in the composition is 35 million to 300 million and in volume less than 25 ml, 20 ml, 15 ml, 10 ml, 7.5 ml, 5 ml or less.
- At least 5% of the viable cells in the composition are CD90 + .
- 10%, 20%, 30%, 40%, 50%, 60%, 70%, 75% or more are CD90 + .
- the cells are about 5-75% viable CD90 + with the remaining cells in the composition being CD45 + .
- the CD45 + cells are CD14 + , CD34 + or VEGFR1 + .
- the composition is substantially free of components used during the production of the cell composition, e.g., cell culture components such as bovine serum albumin, horse serum, fetal bovine serum, enzymatically active harvest reagent (e.g., trypsin) and substantially free of mycoplasma, endotoxin, and microbial contamination .
- cell culture components such as bovine serum albumin, horse serum, fetal bovine serum, enzymatically active harvest reagent (e.g., trypsin) and substantially free of mycoplasma, endotoxin, and microbial contamination .
- the composition contain 10, 5, 4, 3, 2, 1, 0.1, 0.05 or less ⁇ g/ml bovine serum albumin and 5, 4, 3, 2, 1, 0.1, 0.05 g/ml enzymatically active harvest reagent.
- Figure 1 A-D is a series of photographs depicting the process of expanding bone marrow cells in a bioreactor following harvest.
- Figure 2A-B is a pair of graphs depicting the frequency distribution of cell types found in starting bone marrow (A) versus TRC populations following expansion. TRC expansion according methods of the invention also increases the number of early stage cells found in bone marrow. The differences between A and B demonstrate that the frequency of cell types shifts towards stem and progenitor cells following expansion of bone marrow cells into TRC populations in the bioreactor.
- Figure 4 is a graph depicting a Kaplan-Meier survival plot of time to first occurrence of treatment failure, a composite endpoint of major amputation, doubling of wound total surface area, occurrence of de novo gangrene or death. Censored observations are indicated by "+" symbols.
- Figure 5A-B is a pair of graphs depicting a Kaplan-Meier survival plot of amputation- free survival at the first interim analysis timepoint (A) and the final study database lock (B), respectively. Censored observations are indicated by "+" symbols.
- Figure 9 is a graph depicting a Kaplan-Meier survival plot of amputation-free survival (AFS) in patients with wounds at baseline, at the final study database lock.
- AFS amputation-free survival
- Figure 10 is a graph depicting a Kaplan-Meier survival plot of time to treatment failure (TTF) in patients with wounds at baseline, at the final study database lock.
- the present invention is based on the discovery of compositions and methods of producing cells for cell therapy.
- the compositions are a mixed population of cells that are enhanced in stem and progenitor cells that are uniquely suited to human administration for tissue repair, tissue regeneration, and tissue engineering. These cells are referred to herein as "Tissue Repair Cells" or "TRCs.”
- TRCs tissue Repair Cells
- the methods and data presented herein demonstrate that TRCs treat critical limb ischemia in patients/subjects who present a vascular occlusion that cannot be resolved by using a standard method of revascularization.
- TRCs Tissue Repair Cells
- TRCs contain a mixture of cells of hematopoietic, mesenchymal and endothelial cell lineage produced from mononuclear cells.
- the mononuclear cells are isolated from adult, juvenile, fetal or embryonic tissues.
- the mononuclear cells are derived from bone marrow, peripheral blood, umbilical cord blood or fetal liver tissue.
- TRCs are produced from mononuclear cells, for example by an in vitro culture process which results in a unique cell composition having both phenotypic and functional differences compared to the mononuclear cell population that was used as the starting material. Additionally, the TRCs have both high viability and low residual levels of components used during their production.
- the viability of the TRCs is at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95% or more. Viability is measured by methods known in the art such as trypan blue exclusion. This enhanced viability makes the TRC population more effective in tissue repair, as well as enhances the shelf-life and cryopreservation potential of the final cell product.
- components used during production is meant, but not limited, to culture media components such as horse serum, fetal bovine serum and enzyme solutions for cell harvest.
- Enzyme solutions include trypsins (animal-derived, microbial-derived, or recombinant), various collagenases, alternative microbial-derived enzymes, dissociation agents, general proteases, or mixtures of these. Removal of these components provide for safe administration of TRC to a subject in need thereof
- the TRC compositions of the invention contain less than 10, 5, 4, 3, 2, 1 ⁇ g/ml bovine serum albumin; less than 5, 4, 3, 2, 1, 0.9, 0.8, 0.7, 0.6, 0.5 g ml harvest enzymes (as determined by enzymatic activity) and are substantially free of mycoplasma, endotoxin and microbial (e.g., aerobic, anaerobic and fungi) contamination.
- microbial e.g., aerobic, anaerobic and fungi
- substantially free of endotoxin is meant that there is less endotoxin per dose of TRCs than is allowed by the FDA for a biologic, which is a total endotoxin of 5 EU/kg body weight per day, which for an average 70 kg person is 350 EU per total dose of TRCs.
- mycoplasma contamination is determined by subculturing a TRC product sample in broth medium and distributed over agar plates on day 1, 3, 7, and 14 at 37°C with appropriate positive and negative controls. The product sample appearance is compared microscopically, at lOOx, to that of the positive and negative control. Additionally, inoculation of an indicator cell culture is incubated for 3 and 5 days and examined at 600x for the presence of mycoplasma as by epifluorescence microscopy using a DNA-binding fluorochrome. The product is considered satisfactory if the agar and/or the broth media procedure and the indicator cell culture procedure show no evidence of mycoplasma contamination.
- the sterility test to establish that the product is free of microbial contamination is based on the U.S. Pharmacopedia Direct Transfer Method. This procedure requires that a pre-harvest medium effluent and a pre-concentrated sample be inoculated into a tube containing tryptic soy broth media and fluid thioglycollate media. These tubes are observed periodically for a cloudy appearance (turbidity) for a 14 day incubation. A cloudy appearance on any day in either medium indicate contamination, with a clear appearance (no growth) testing substantially free of contamination.
- Results are the average ⁇ SEM from 8 clinical-scale experiments.
- the cells of the TRC composition have been characterized by cell surface marker expression.
- Table 2 shows the typical phenotype measured by flow cytometry for starting BM MNCs and TRCs. These phenotypic and functional differences highly differentiate TRCs from the mononuclear cell starting compositions.
- M mesenchymal lineage
- H hematopoietic lineage
- E endothelial lineage
- TRCs are highly enriched for CD90 + cells compared to the mononuclear cell population from which they are derived.
- the cells in the TRC composition are at least 5%, 10%, 25%, 50%, 75%, or more CD90 + .
- the remaining cells in the TRC composition are CD45 + .
- the cells in the TRC composition are about 5-75% viable CD90 + .
- at least 5%, 10%, 15% , 20%, 25%, 30%, 40%, 50%, 60% or more of the CD90 + are also CD15 + (Table 3).
- the CD90 + are also CD105 + .
- the CD90 + population in bone marrow mononuclear cells is typically less than 1% with the resultant CD45 + cells making up greater than 99% of the nucleated cells in BMMNCs
- mesenchymal stem cells are highly purified for CD90 + (greater than 95% CD90 + ), with very low percentage CD45 + (if any).
- Adipose-derived stem cells are more variable but also typically have greater than 95% CD90 + , with almost no CD45 + blood cells as part of the composition.
- MSCs multi-Potent Adult Progenitor Cells
- MSCs Multi-Potent Adult Progenitor Cells
- TRCs stem cells being used are highly purified cell types including CD34 + cells, AC133 + cells, and CD34 + lin " cells, which by nature have little to no CD90 + cells as part of the composition and thus are substantially different from TRCs.
- CD34 + cells CD34 + cells
- AC133 + cells AC133 + cells
- CD34 + lin " cells which by nature have little to no CD90 + cells as part of the composition and thus are substantially different from TRCs.
- Cell marker analysis have also demonstrated that the TRCs isolated according to the methods of the invention have higher percentages of CD14 + auto + , CD34 + and VEGFR + cells.
- T-cells (CD45 + CD4 + CD25 + ) regulate innate inflammatory response after injury. (Murphy T.J., et al, J. Immunol., 174:2957-2963 (2005)). The T-cells are also responsible for maintenance of self tolerance and prevention and suppression of autoimmune disease. (Sakaguchi S. et al, Immunol. Rev., 182: 18-32 (2001); Tang Q., et al, J. Exp. Med., 199:1455-1465 (2004)) The T-cells also induce and maintain transplant tolerance (Kingsley C.I., et al J.
- CD45 + CD90 + CD105 + express IDO and inhibit T-cell activation (Meisel R., et al, Blood, 103:4619-4621 (2004); Krampera M., et al, Stem Cells, (2005)) as well as induce antiinflammatory activity (Aggarwal S. and Pittenger M.F., Blood, 105: 1815-1822 (2005)).
- TRCs also show increased expression of programmed death ligand 1 (PDL1). Increased expression of PDL1 is associated with production of the anti-inflammatory cytokine IL-10. PDL1 expression is associated with a non-inflammatory state. TRCs have increased PDL1 expression in response to inflammatory induction, showing another aspect of the antiinflammatory qualities of TRCs.
- PDL1 programmed death ligand 1
- TRCs in contrast to BM MNCs also produce at least five distinct cytokines and one regulatory enzyme with potent activity both for wound repair and controlled down-regulation of inflammation Specifically, TRCs produce 1) Interleukin-6 (IL-6), 2) Interleukin-10 (IL-10), 3) vascular endothelial growth factor (VEGF), 4) monocyte chemoattractant protein- 1 (MCP-1) and, 5) interleukin-1 receptor antagonist (IL-lra). The characteristics of these five cytokines is summarized in Table 5, below.
- TRCs Additional characteristics include a failure to spontaneously produce, or very low-level production of certain pivotal mediators known to activate the Thl inflammatory pathway including interleukin- alpha (IL-la), interleukin-beta (IL- ⁇ ) interferon-gamma (IFN- ⁇ ) and most notably interleukin- 12 (IL-12).
- IL-la interleukin- alpha
- IL- ⁇ interleukin-beta
- IFN- ⁇ interferon-gamma
- IL-12 interleukin- 12
- the TRCs neither produce these latter Thl -type cytokines spontaneously during medium replacement or perfusion cultures nor after intentional induction with known inflammatory stimuli such as bacterial lipopolysaccharide (LPS).
- LPS bacterial lipopolysaccharide
- the TRCs produced by the current methods produce more of the anti-inflammatory cytokines IL-6 and IL-10 as well as less of the inflammatory cytokine IL-12.
- TRCs are inducible for expression of a key immune regulatory enzyme designated indoleamine-2,-3 dioxygenase (IDO).
- IDO indoleamine-2,-3 dioxygenase
- the TRCs according to the present invention express higher levels of IDO upon induction with interferon- ⁇ . IDO has been demonstrated to down-regulate both nascent and ongoing inflammatory responses in animal models and humans (Meisel R., et al, Blood, 103:4619-4621 (2004); Munn D.H., et al, J. Immunol., 156:523-532 (1996); Munn D.H., et al J. Exp. Med. 189: 1363-1372 (1999); Munn D.H. and Mellor A.L., Curr. Pharm. Des., 9:257-264 (2003); Mellor A.L. and Munn D.H., J. Immunol., 170:5809-5813 (2003)).
- TRCs are highly enriched for a population of cells that co-express CD90 and CD15.
- CD90 is present on stem and progenitor cells that can differentiate into multiple lineages. These cells are a heterogeneous population of cells that are at different states of differentiation. Cell markers have been identified on stem cells of embryonic or fetal origin that define the differentiation state of the cell. One of these markers, SSEA-1, also referred to as CD15, is found on mouse embryonic stem cells, but is not expressed on human embryonic stem cells. It has however been detected in neural stem cells in both mice and human. CD 15 is also not expressed on purified mesenchymal stem cells derived from human bone marrow or adipose tissue (Table 6). Thus, the cell population in TRCs that co-expresses both CD90 and CD 15 is a unique cell population and may define a the stem-like state of the CD90 adult-derived cells.
- the cell population expressing both CD90 and CD 15 may be further enriched.
- further enriched is meant that the cell composition contains 5%, 10%, 25%, 50%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% 99% or 100% CD90 + CD15 + cells.
- TRCs can be further enriched for CD90 + CD15 + cells by methods known in the art such as positive or negative selection using antibodies direct to cell surface markers.
- the TRCs that have been further enriched for CD90 + CD15 + cells are particularly useful in cardiac repair and regeneration.
- TRCs are isolated from any mammalian tissue that contains bone marrow mononuclear cells (BM MNC). Suitable sources for BM MNC is peripheral blood, bone marrow, umbilical cord blood or fetal liver. Blood is often used because this tissue is easily obtained. Mammals include for example, a human, a primate, a mouse, a rat, a dog, a cat, a cow, a horse or a pig.
- BM MNC bone marrow mononuclear cells
- the culture method for generating TRCs begins with the enrichment of BM MNC from the starting material (e.g., tissue) by removing red blood cells and some of the polynucleated cells using a conventional cell fractionation method. For example, cells are fractionated by using a FICOLL® density gradient separation.
- the volume of starting material needed for culture is typically small, for example, 40 to 50 mL, to provide a sufficient quantity of cells to initiate culture. However, any volume of starting material may be used.
- Nucleated cell concentration is then assessed using an automated cell counter, and the enriched fraction of the starting material is inoculated into a biochamber (cell culture container).
- the number of cells inoculated into the biochamber depends on its volume.
- TRC cultures which may be used in accordance with the invention are performed at cell densities of from 10 4 to 10 9 cells per ml of culture. When a Aastrom Replicell Biochamber is used 2-3 x 10 total cells are inoculated into a volume of approximately 280 mL.
- a biochamber Prior to inoculation, a biochamber is primed with culture medium.
- the medium used in accordance with the invention comprises three basic components.
- the first component is a media component comprised of IMDM, MEM, DMEM, RPMI 1640, Alpha Medium or McCoy's Medium, or an equivalent known culture medium component.
- the second is a serum component which comprises at least horse serum or human serum and may optionally further comprise fetal calf serum, newborn calf serum, and/or calf serum.
- serum free culture mediums known in the art may be used.
- the third component is a corticosteroid, such as hydrocortisone, cortisone, dexamethasone, solumedrol, or a combination of these, preferably hydrocortisone.
- the culture medium further comprises B7H3 polypeptides, VSIG4 polypeptides or a combination of both.
- the culture medium consists of IMDM, about 10% fetal bovine serum, about 10% horse serum, about 5 ⁇ hydrocortisone, and 4mM L-Glutamine.
- the cells and media are then passed through the biochamber at a controlled ramped perfusion schedule during culture process.
- the cells are cultured for 2, 4, 6, 8, 10, 12, 14, 16 or more days.
- the cells are cultured for less than 12 days. Not to be bound by theory, but it is thought that the addition of B7H3 polypeptides, VSIG4 polypeptides or both will allow for the rapid expansion of TRCs, in particular the CD45 + , CD31 + , CD14 + , and auto + cell population. This rapid expansion will greatly reduce culturing time which is a particular advantage when manufacturing cell suitable for transplantation into humans.
- These cultures are typically carried out at a pH which is roughly physiologic, i.e. 6.9 to 7.6.
- the medium is kept at an oxygen concentration that corresponds to an oxygen-containing atmosphere which contains from 1 to 20 vol. percent oxygen, preferably 3 to 12 vol. percent oxygen.
- the preferred range of 0 2 concentration refers to the concentration of 0 2 near the cells, not necessarily at the point of 0 2 introduction which may be at the medium surface or through a membrane.
- Standard culture schedules call for medium and serum to be exchanged weekly, either as a single exchange performed weekly or a one-half medium and serum exchange performed twice weekly.
- the nutrient medium of the culture is replaced, preferably perfused, either continuously or periodically, at a rate of about 1 ml per ml of culture per about 24 to about 48 hour period, for cells cultured at a density of from 2xl0 6 to lxlO 7 cells per ml.
- the same medium exchange rate may be used.
- the present medium replacement rate may be expressed as 1 ml of medium per 10' cells per about 24 to about 48 hour period.
- the medium exchange rate may be increased proportionality to achieve a constant medium and serum flux per cell per unit time
- Bone marrow (BM) aspirates are diluted in isotonic buffered saline (Diluent 2, Stephens Scientific, Riverdale, NJ), and nucleated cells are counted using a Coulter ZM cell counter (Coulter Electronics, Hialeah, FL).
- Erythrocytes are lysed using a Manual Lyse (Stephens Scientific), and mononuclear cells (MNC) are separated by density gradient centrifugation (Ficoll-Paque ® Plus, Pharmacia Biotech, Uppsala, Sweden) (specific gravity 1.077) at 300g for 20 min at 25°C.
- MNC mononuclear cells
- LTBMC long-term BM culture medium
- IMDM Iscove's modified Dulbecco's medium
- the cells are harvested, for example using trypsin, and washed to remove the growth medium.
- the cells are resuspended in a pharmaceutical grade electrolyte solution, for example Isolyte (B. Braun Medical Inc., Bethlehem, PA) supplemented with serum albumin.
- the cells are washed in the biochamber prior to harvest using the wash harvest procedure described below.
- the cells are concentrated and cryopreserved in a biocompatible container, such as 250 ml cryocyte freezing containers (Baxter Healthcare Corporation, Irvine, CA) using a cryoprotectant stock solution containing 10% DMSO (Cryoserv, Research Industries, Salt Lake City, UT), 10% HSA (Michigan Department of Public Health, Lansing, MI), and 200 ⁇ g ml recombinant human DNAse (Pulmozyme ,
- cryocyte freezing container is transferred to a precooled cassette and cryopreserved with rate-controlled freezing (Model 1010, Forma Scientific, Marietta, OH).
- rate-controlled freezing Model 1010, Forma Scientific, Marietta, OH
- Frozen cells are immediately transferred to a liquid nitrogen freezer (CMS-86, Forma Scientific) and stored in the liquid phase.
- Preferred volumes for the concentrated cultures range from about 5 mL to about 15 ml. More preferably, the cells are concentrated to a volume of 7.5 mL.
- the cells When harvested from the biochamber the cells reside in a solution that consists of various dissolved components that were required to support the culture of the cells as well as dissolved components that were produced by the cells during the culture. Many of these components are unsafe or otherwise unsuitable for patient administration. To create cells ready for therapeutic use in humans it is therefore required to separate the dissolved components from the cells by replacing the culture solution with a new solution that has a desired composition, such as a pharmaceutical-grade, injectable, electrolyte solution suitable for storage and human
- a significant problem associated with many separation processes is cellular damage caused by mechanical forces applied during these processes, exhibited, for instance, by a reduction in viability and biological function of the cells and an increase in free cellular DNA and debris. Additionally, significant loss of cells can occur due to the inability to both transfer all the cells into the separation apparatus as well as extract all the cells from the apparatus.
- centrifugal separation is the COBE 2991 Cell Processor (COBE BCT) and an example of a filtration separation is the CYTOMATE® Cell Washer (Baxter Corp) (Table 7). Both are commercially available state-of-the-art automated separation devices that can be used to separate (wash) dissolved culture components from harvested cells. As can be seen in Table 7, these devices result in a significant drop in cell viability, a reduction in the total quantity of cells, and a shift in cell profile due to the preferential loss of the large and fragile CD14 + auto + subpopulation of TRCs.
- the invention described in this disclosure overcomes all of these limitations in the current art by implementing a separation process to wash the cells that minimizes exposure of the cells to mechanical forces and minimizes entrapment of cells that cannot be recovered. As a result, damage to cells (e.g. reduced viability or function), loss of cells, and shift in cell profile are all minimized while still effectively separating unwanted dissolved culture components.
- the separation is performed within the same device that the cells are cultured in which eliminates the added risk of contamination by transfer and separation using another apparatus.
- the wash process according to the invention is described below.
- wash-harvest technique reverses the order and provides a unique means to complete all separation (wash) steps prior to harvest of the cells from the biochamber.
- a new liquid of desired composition may be introduced, preferably at the center of the biochamber and preferably at a predetermined, controlled flow rate. This results in the liquid being displaced and expelled along the perimeter of the biochamber, for example, through apertures, which may be collected in the waste bag.
- the diameter of the liquid space in the biochamber is about 33 cm
- the height of the liquid space is about 0.33 cm
- the flow rates of adding rinsing and/or harvesting fluids to the biochamber is about 0.03 to 1.0 volume exchanges (VE) per minute and preferably 0.50 to about 0.75 VE per minute. This substantially corresponds to about 8.4 to about 280 mL/min and preferably 140 to about 210 ml/min.
- the flow rates and velocities aid in insuring that a majority of the cultured cells are retained in the biochamber and not lost into the waste bag and that an excessively long time period is not required to complete the process.
- the quantity of cells in the chamber may range from 10 4 to 10 8 cell/mL.
- the quantity may range fromlO 5 to 10 6 cells/mL, corresponding to 30 to 300 million total cells for the biochamber dimensions above.
- the following process in harvesting the cultured cells from the biochamber, the following process may be followed, and is broadly outlined in Table 8, below.
- the solutions introduced into the biochamber are added into the center of the biochamber.
- the waste media bag 76 may collect corresponding fluid displaced after each step where a fluid or gas is introduced into the biochamber.
- the biochamber is filled with conditioned culture medium (e.g., IMDM, 10% FBS, 10% Horse Serum, metabolytes secreted by the cells during culture) and includes between about 30 to about 300 million cells.
- a 0.9% NaCl solution (“rinse solution”) may then be introduced into the biochamber at about 140 to 210 mL per minute until about 1.5 to about 2.0 liters of total volume has been expelled from the biochamber (Step 1).
- the rinse solution is replaced by harvest solution.
- a harvest solution is typically an enzyme solution that allows for the detachment of cells adhered to the culture surface.
- Harvest solutions include for example 0.4% Trypsin/EDTA in 0.9% NaCl that may be introduced into the biochamber at about 140 to 210 mL per minute until about 400 to about 550 ml of total volume has been delivered (Step 2). Thereafter, a predetermined period of time elapses (e.g., 13-17 minutes) to allow enzymatic detachment of cells adhered to the culture surface of the biochamber (Step 3).
- Isolyte (B Braun) supplemented with 0.5% HSA may be introduced at about 140 to 210 mL per minute until about 2 to about 3 liters of total volume has been delivered, to displace the enzyme solution (Step 4).
- Step 5 To reduce the volume collected, some of the Isolyte solution is preferably displaced using a gas (e.g., air) which is introduced into the biochamber at a disclosed flow rate (Step 5). This may be used to displace approximately 200 to 250 cc of the present volume of the biochamber.
- a gas e.g., air
- the biochamber may then be agitated to bring the settled cells into solution (Step 6).
- This cell suspension may then be drained into the cell harvest bag 70 (or other container) (Step 7).
- An additional amount of the second solution may be added to the biochamber and a second agitation may occur in order to rinse out any other residual cells (Steps 8 & 9).
- This final rinse may then be added to the harvest bag 70 (Step 10).
- TRCs Tissue Repair Cells
- administration of a TRC composition delays or prevents the progression of no-option CLI over a period of time, which may include the death of the patient (that is not a result of no-option CLI).
- administration of a TRC composition improves a symptom of no-option CLI, thereby improving the quality of life for the individual.
- Critical Limb Ischemia or CLI is a severe obstruction of the arteries, which decreases blood flow to the extremities (hands, feet and legs). Irs fact, blood flow is so minimal thai when a patient is diagnosed with CLI, he or she presents severe pain thai often coincides with the appearance of open wounds that cannot heal (including skin ulcers or sores), The pain caused by CLI is constant and pervades all aspects of life. CLI-associated pain is most noticeable to the patient when he or she is at rest, and, therefore, this pain is also referred to as "rest pain " .
- Temporary relief from rest pain can be found by moving the limb or walking for a short period of time.
- No-option CLI is a form of CLI in which arterial blood flow cannot be restored to the affected limb by using any known or standard method of revascularization.
- a no-option CLI patient suffers from atherosclerosis or arteriosclerotic vascular disease (AVSD), a condition in which an artery wall thickens as a result of the accumulation of fatty materials such as cholesterol.
- AVSD arteriosclerotic vascular disease
- the subject presents a vascular occlusion. If the occlusion is complete, then the individual could be diagnosed with no-option CLI based upon the singular disorder.
- an individual develops no-option CLI for variety of reasons, most of which have a basis in that individual's unique physiology.
- the patient has other underlying medical conditions, such as obesity or diabetes in addition to atherosclerosis.
- a patient having multiple medical conditions not only presents a vascular occlusion, but may also present additional obstacles to application of standard methods of revascularization, which include either open surgical or percutaneous endovascular procedures. For instance, the location of the occlusion may prevent standard treatment.
- the patient is obese, diabetic, or aged, the patient may not be otherwise healthy enough for surgery or the subsequent recovery. Because the patient has no acceptable alternative to revascularization, he or she falls within the scope of no-option CLI.
- TRCs are delivered to no-option CLI patients using the procedures provided in Examples 1 and 2.
- the invention provides a method of treating critical limb ischemia (CLI) in a subject, wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination, thereby improving or preventing the clinical consequence of critical limb ischemia (CLI) in a subject,
- the standard method of revascularization is an open surgical procedure or a percutaneous endovascular procedure.
- the presence of a vascular occlusion that cannot be resolved by using a standard method of revascularization, i.e., the presentation of no-option CLI, may be determined by physical examination, angiographic imaging, color flow duplex ultrasound, or any combination thereof.
- the subject may present a vascular occlusion in one or more upper or lower extremities, including any portion thereof.
- the subject may present recurring ischemic rest pain for at least 2 weeks, ulceration, or gangrene with absent pulses in one or more extremities.
- the subject may further present recurring ischemic rest pain for at least 2 weeks, ulceration, or gangrene in the foot or toe with absent pedal pulses, and with either a toe systolic pressure of equal to or less than 50 mm Hg or ankle systolic pressure of equal to or less than 70 mm Hg.
- no-option CLI includes improving mobility, decreasing pain, improving wound healing, decreasing wound size, and delaying tissue loss, amputation, and death.
- the treatment for no-option CLI can be a cure, for instance, in an otherwise healthy individual, the measure of success for treating a subject with no-option CLI in the average subject includes ameliorating an existing symptom or delaying the onset of a worse symptom.
- the methods described herein prevent a clinical consequence from occurring when the patient either experiences recovery or when the adverse events associated with no-option CLI are delayed for such a period of time that the patient avoids its occurrence for the duration of his or her life. In an elderly patient, this period of survival may be shorter than in a younger patient, however, in either situation, the endpoint remains recovery or morbidity (by a cause unrelated to no-option CLI). Recovery is defined by either revascularization or sufficient reanimation of the affected limb to be functional.
- a functional recovery would include the ability of that individual to support his or her weight, to even to surrender the use of the cane, walker, or wheelchair, depending upon the degree of revascularization. It is contemplated that a subject who regains sufficient function in an affected limb, can sustain motion in this limb and, therefore, through further treatment and physical therapy, permanently avoid amputation.
- a clinical consequence of no-option CLI is an adverse event that, without treatment, will inevitably occur as the disease progresses.
- the term clinical consequence is used to encompass both natural consequences, such as increased pain, wound size, decreased healing, de novo gangrene, and death, and medical consequences such as amputation.
- Medical consequences are adverse events, compared to what a healthy person might encounter, however, they include necessary interventions to prolong life or improve the quality of life for a patient (e.g. surgery and amputation).
- Exemplary clinical consequences of no-option critical limb ischemia include, but are not limited to, increased rest pain, decreased mobility of a limb (arm or leg), ulceration, increased wound size (doubling of wound size), decreased or impaired wound healing, de novo gangrene, decreased or absent pulse at extremity, tissue loss (tissue necrosis), amputation (for instance, of a digit, such as a finger or toe, which would not constitute a major amputation), major amputation (defined as, for example, an amputation at or above the talus on the leg), or death.
- Decreased function of an affected limb includes, but is not limited to, decreased range of motion, decreased strength, or decreased endurance for physical exertion of the limb.
- the limb is a leg and a decreased function of an affected limb includes decreased walking distance or decreased walking time.
- treatment of the subject having no-option CLI achieves a clinical goal.
- Exemplary clinical goals include, but are not limited to, decreased pain, increased function of an affected limb, decreased wound size, increased wound healing, delay or prevention of de novo gangrene, delay or prevention of amputation, or increased survival.
- decreased pain is determined by comparing a demand from the subject for administration of a pain medicine or a dosage of a pain medication from a time period prior to administration of the composition to a demand from the subject for administration of a pain medicine or a dosage of a pain medication from a time point following administration of the composition, wherein a decreased demand or a decreased dosage indicates that the treatment decreased the pain of the subject following administration of the composition.
- increased function of an affected limb is determined by comparing a range of motion, a strength, or an endurance measurement for physical exertion of the limb from a time period prior to administration of the composition to a range of motion, a strength, or an endurance measurement for physical exertion of the limb from a time point following administration of the composition, wherein an increased range of motion, increased strength, or increased endurance measurement indicates that the treatment increased the function of the affected limb of the subject following administration of the composition.
- decreased wound size is determined by comparing an area, circumference, or depth measurement of a wound from a time period prior to administration of the composition to an area, circumference, or depth measurement of a wound from a time point following administration of the composition, wherein a decreased area, circumference, or depth measurement indicates that the treatment decreased size of a wound following administration of the composition.
- increased wound healing is determined by comparing a measurement of active inflammation, angiogenesis, collagen disposition, fibroplasia, granulation tissue formation, epithelialization, contraction, or remodeling of a wound from a time period prior to administration of the composition to a measurement of active inflammation, angiogenesis, collagen disposition, fibroplasias, granulation tissue formation, epithelialization, contraction, or remodeling of a wound from a time point following administration of the composition, wherein an increased measurement of active inflammation, angiogenesis, collagen disposition, fibroplasia, granulation tissue formation, epithelialization, contraction, or remodeling indicates that the treatment increased wound healing following administration of the composition.
- delay or prevention of de novo gangrene is determined by comparing a measurement of tissue necrosis from a time period prior to administration of the composition to a measurement of tissue necrosis from a time point following administration of the composition, wherein an identical or decreased
- tissue necrosis indicates that the treatment delayed or prevented the formation of de novo gangrene following administration of the composition.
- delay or prevention of amputation is determined by comparing the prognosis for amputation in the subject from a time period prior to administration of the composition to the prognosis for either amputation in the subject following administration of the composition, wherein an increase in the time required until amputation or a cancellation of the amputation procedure due to recovery indicates that the treatment delayed or prevented the amputation of the affected limb, respectively.
- increased survival is determined by comparing the prognosis for survival in the subject from a time period prior to administration of the composition to the prognosis for survival in the subject following administration of the composition, wherein an increase in predicted survival time indicates that the treatment increased survival of the subject following administration of the composition.
- the TRC composition also known as ixmyelocel-T, is administered by intramuscular or intravascular injection at one or more sites. Preferably, the composition is administered by intramuscular injection at approximately 20 sites.
- the TRC composition may be delivered through a wide range of needle sizes, from large 16 gauge needles to very small 30 gauge needles, as well as very long 28 gauge catheters for minimally invasive procedures.
- the cells of the composition are derived from mononuclear cells. These mononuclear cells are derived from bone marrow, peripheral blood, umbilical cord blood or fetal liver. [0130] Optionally, the cells of the composition are in formulated or provided in a pharmaceutical-grade electrolyte solution suitable for human administration.
- the composition is substantially free of horse serum and/or fetal bovine serum.
- the CD90 + cells of the composition co- express CD 15.
- the CD45 + cells of the composition are CD14 + , CD34 + or VEGFRl + .
- the total number of viable cells in the composition is between 35 million and 300 million.
- the composition contains an average of between 90-180 x 10 6 viable cells.
- the cells may be suspended in a volume of equal to or less than 15 milliliters, equal to or less than 10 milliliters, or equal to or less than 7.5 milliliters.
- the invention provides a method of increasing amputation-free survival in a subject diagnosed with critical limb ischemia (CLI), wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination.
- CLI critical limb ischemia
- the amputation-free survival is increased in the treated subject when compared to an untreated subject, wherein the untreated subject is also diagnosed with critical limb ischemia (CLI) and also presents a vascular occlusion that cannot be resolved by using a standard method of revascularization.
- Amputation- free survival is defined as the time of administration of the composition until an amputation is performed, the subject dies, or the combination occurs.
- the invention provides a method of preventing major amputation in a subject diagnosed with critical limb ischemia (CLI), wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 ⁇ g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination.
- CLI critical limb ischemia
- the vascular occlusion may occur in a leg.
- the major amputation is an amputation at or above the talus on the leg.
- a major amputation is prevented from the time of administration of the composition until the passage of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, or 25 years.
- a major amputation is prevented because the extremity is revascularized, as confirmed, for instance, by physical examination, angiographic imaging, color flow duplex ultrasound, or any combination thereof.
- the invention provides a method of delaying the onset of de novo gangrene, tissue loss, amputation, or death in a subject diagnosed with critical limb ischemia (CLI), wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination.
- CLI critical limb ischemia
- the onset of de novo gangrene, tissue loss, amputation, or death is delayed in the treated subject when compared to an untreated subject, wherein the untreated subject is also diagnosed with critical limb ischemia (CLI) and also presents a vascular occlusion that cannot be resolved by using a standard method of revascularization.
- CLI critical limb ischemia
- the term amputation includes both minor and major amputation.
- the invention provides a method of increasing survival probability in a subject diagnosed with critical limb ischemia (CLI), wherein the subject presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, including administering to the subject an isolated cell composition for tissue repair including a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of the cells is at least 80% and the composition contains: a) about 5-75% viable CD90 + cells with the remaining cells in the composition being CD45 + ; b) less than 2 ⁇ g/ml of bovine serum albumin; c) less than 1 ⁇ g/ml of a enzymatically active harvest reagent; and d) substantially free of mycoplasma, endotoxin, and microbial contamination.
- CLI critical limb ischemia
- the survival probability is increased in the treated subject when compared to an untreated subject, wherein the untreated subject is also diagnosed with critical limb ischemia (CLI) and also presents a vascular occlusion that cannot be resolved by using a standard method of revascularization.
- Survival probability is an alternative method of expressing the time to treatment failure, or the likelihood that the treatment will be successful.
- the composition is administered to a subject who presents a vascular occlusion that cannot be resolved by using a standard method of revascularization, in combination with another therapy. For instance, if the subject suffers from an underlying atherosclerosis in the limb undergoing treatment, or in another part of his or her body, the composition is administered in combination with a pharmaceutical agent.
- Contemplated pharmaceutical agents reduce lipids (lipid or cholesterol reduction therapy), reduce platelet aggregation or platelet attachment to the walls of the vasculature (anti-platelet therapy), or reduce blood pressure (anti-hypertensive therapy).
- the subject of the present methods may have a wound associated with no-option CLI on the treated limb, or on another part of his or her body.
- the composition is administered in combination with topical or systemic wound care.
- wound care includes, but is not limited to, pharmaceutical agents to decrease infection (like antibiotics), decrease inflammation, promote healing (antioxidants), and promote vascularization (pro-angiogenic factors); matrices or scaffolds to provide a substrate upon which to grow tissue for the wound; and surgical intervention to removal of dead, damaged, or infected tissue (debridement).
- the methods provided herein may also be used in combination with treatments for obesity (for instance, drugs including olitstat and the non-prescription version, alii), heart disease (for instance, drugs used to combat high cholesterol or high blood pressure), and diabetes (for example, insulin for type I and weight-loss therapy for type II).
- obesity for instance, drugs including olitstat and the non-prescription version, alii
- heart disease for instance, drugs used to combat high cholesterol or high blood pressure
- diabetes for example, insulin for type I and weight-loss therapy for type II.
- TRCs can be administered as a pharmaceutically or physiologically acceptable preparation or composition containing a physiologically acceptable carrier, excipient, or diluent, and administered to the tissues of the recipient organism of interest, including humans and non-human animals.
- TRC-containing composition can be prepared by resuspending the cells in a suitable liquid or solution such as sterile physiological saline or other physiologically acceptable injectable aqueous liquids.
- suitable liquid or solution such as sterile physiological saline or other physiologically acceptable injectable aqueous liquids.
- the amounts of the components to be used in such compositions can be routinely determined by those having skill in the art.
- the TRCs can be administered by parenteral routes of injection, including subcutaneous, intravenous, intramuscular, and intrasternal. Other modes of administration include, but are not limited to, intrathecal, intracutaneous, and percutaneous. In one embodiment of the present invention, administration of the TRCs can be mediated by endoscopic surgery.
- the composition is in sterile solution or suspension or can be resuspended in pharmaceutically- and physiologically-acceptable aqueous or oleaginous vehicles, which may contain preservatives, stabilizers, and material for rendering the solution or suspension isotonic with body fluids (i.e. blood) of the recipient.
- excipients suitable for use include water, phosphate buffered saline, pH 7.4, 0.15 M aqueous sodium chloride solution, dextrose, glycerol, dilute ethanol, and the like, and mixtures thereof.
- Illustrative stabilizers are polyethylene glycol, proteins, saccharides, amino acids, inorganic acids, and organic acids, which may be used either on their own or as admixtures.
- the amounts or quantities, as well as the routes of administration used, are determined on an individual basis, and correspond to the amounts used in similar types of applications or indications known to those of skill in the art.
- the TRC can be administered to body tissues, including blood vessel, muscle, skeletal muscle, joints, and limb.
- the number of cells in a TRC suspension and the mode of administration may vary depending on the site and condition being treated. As non-limiting examples, in accordance with the present invention, about 35-300xl0 6 TRCs are injected to effect tissue repair. Consistent with the Examples disclosed herein, a skilled practitioner can modulate the amounts and methods of TRC-based treatments according to requirements, limitations, and/or optimizations determined for each case.
- the TRC pharmaceutical composition comprises between about 8 and 54% CD90 + cells and between about 46 and 92% CD45 + cells.
- the TRC pharmaceutical composition preferably contains between about 35xl0 6 and 300x10 6 viable nucleated cells and between about 7xl0 6 and 75xl0 6 viable CD90 + cells.
- the TRC pharmaceutical compositional preferably has less than 0.5 EU/ml of endotoxin and no bacterial or fungal growth.
- a dosage form of TRCs is comprised within 4.7-7.3 mL of pharmaceutically acceptable aqueous carrier.
- the preferred suspension solution is Multiple Electrolyte Injection Type 1 (USP/EP).
- Each 100 mL of Multiple Electrolyte Injection Type 1 contains 234 mg of Sodium Chloride, USP (NaCl); 128 mg of Potassium Acetate, USP (C 2 H 3 KO 2 ); and 32 mg of Magnesium Acetate Tetrahydrate (Mg(C 2 H 3 0 2 ) 2 *4H 2 0). It contains no antimicrobial agents.
- the pH is adjusted with hydrochloric acid. The pH is 5.5 (4.0 to 8.0).
- the Multiple Electrolyte Injection Type 1 is preferably supplemented with 0.5% human serum albumin (USP/EP).
- the TRC pharmaceutical composition is stored at 0-12 °C, unfrozen.
- TRCs may be manufactured and processed for delivery to patients using the described processes where the final formulation is the TRCs with all culture components substantially removed to the levels deemed safe by the FDA. It is critical for the cells to have a final viability greater than 70%, however the higher the viability of the final cell suspension the more potent and efficacious the final cell dose will be, and the less cellular debris (cell membrane, organelles and free nucleic acid from dead cells), so processes that enhance cell viability while maintaining the substantially low culture and harvest components, while maintaining closed aseptic processing systems are highly desirable.
- Example 1 Design and Methods for Trial of Expanded Autologous Bone Marrow
- the secondary objective of this study was to investigate the efficacy of ixmyelocel-T in treating CLI, and in particular, no-option CLI.
- the secondary endpoints of the study were time to first occurrence of treatment failure (TTF; major amputation of the treated leg, all-cause mortality, doubling of the total wound surface area from baseline, and de novo gangrene.
- CLI Critical Limb Ischemia
- 5-year, and 10-year mortality rates are approximately 35%, 70%, and 100%, respectively.
- 40 to 50% of patients will undergo major limb amputation within 6 to 12 months.
- the CLI patient population is predominant elderly, and therefore, the ability of this population to successfully rehabilitate and maintain an independent living status following major limb amputation is poor.
- CLI is treated using a multi-faced approach.
- the vascular occlusion may be treated pharmacologically with lipid reduction, anti-platelet and anti-hypertensive therapies.
- the resultant wounds are treated by standard methods including surgical debridement.
- Revascularization continues to be the most important method of treatment. Standard methods of revascularization include either open surgical procedures or percutaneous endovascular approaches.
- TRCs-Autologous Bone Marrow Cells in Patients with Peripheral Arterial Disease to Treat Critical Limb Ischemia
- RESTORE-CLI is a prospective, randomized, double-blinded, placebo-controlled multi-center study that compares intramuscular injections of expanded autologous bone marrow cells ("Tissue Repair Cells” or TRCs) that are suspended in physiological electrolyte solution with injections of the same electrolyte solution without cells in patients with lower extremity critical limb ischemia (CLI).
- TRCs expanded autologous bone marrow cells
- the study was sponsored by Aastrom Biosciences Inc. in Ann Arbor, Michigan.
- DSMB Data Safety Monitoring Board
- Eligible patients were men and women 18 to 90 years of age with a diagnosis of CLI of the lower extremities defined as persistent, recurring ischemic rest pain for at least 2 weeks and/or ulceration or gangrene of the foot or toe with absent palpable pedal pulses with toe systolic pressure ⁇ 50 mm Hg or ankle systolic pressure ⁇ 70 mm Hg.
- Patients with flat or barely pulsatile pulse volume recording (PVR) and higher systolic blood pressures could be included based on sponsor review.
- Patients with infrainguinal occlusive disease without acceptable options for revascularization as determined by the site principles investigator was confirmed by angiographic imaging or color flow duplex ultrasound within 6-months prior to randomization were eligible. Establishment of controlled blood pressure with anti-hypertensive therapy as necessary, adequate anti-platelet and statin therapy was required prior to entry.
- Main exclusion criteria were poorly controlled diabetes (defined as, HbAi c > 10%); known aortoiliac disease with > 50% stenosis; wound with exposed tendon or bone (or a wound severity of greater than Grade 3 on the Wagner Wound Scale); known failed ipsilateral revascularization procedure within 2 weeks prior to randomization (defined as failure to restore adequate circulation, i.e. the procedure did not achieve an increase in ABI of 0.15 or more, substantial improvement in PVR, or clinical improvement); previous amputation of the talus or above in the target limb; infection of the involved extremity (manifested by, for example, fever, purulence, and severe cellulitis); and any active wet gangrenous tissue.
- Patients were centrally randomized 2:1 (treatment:control). Visits were scheduled on day minus 14 (bone marrow or sham aspiration), day 0 (injection), days 3 and 7, and months 3, 6, 9 and 12.
- Enrollment began at 20 clinical sites in the US in April 2007. The planned study population size was originally up to 150 patients. By November 2009, 33 patients had the opportunity to complete the trial (the 12-month follow-up visit), and were included in a prospectively planned first interim analysis. The first interim analysis was expanded to include 13 additional patients that had completed 6 months of follow-up at that time. Only the set of 32 patients completing 12 months and the set of 14 patients completing 6 months of follow-up by November 2009 were unblinded and included in the interim analysis and reported. Enrollment was subsequently halted and all enrolled patients were followed until completing the 12 month efficacy end-point. The final database lock for the study occurred in May 2011.
- TRC product was generated in a single-pass perfusion biochamber over approximately 12 days and then transported to the clinical site in a shipping container designed to maintain hypothermic storage conditions (between 0-12°C)(Dennis et al. Stem Cells. 2007 Oct; 25(10); 2575-82).
- TRCs are a mixture of nucleated cells cultured from the patient's bone marrow with high viability. TRCs are primarily composed of two cell phenotypes: mesenchymal stem and progenitor cells defined by the CD90 + cell surface marker, and hematopoietic and endothelial stem and progenitor cells, defined by the CD45 + cell surface marker.
- the overall cell viability as measured by membrane integrity by dye exclusion is greater than or equal to 70%.
- the cells are suspended in a physiological solution of HypoThermosol ® (BioLife Solutions) and Isolyte (B. Braun) supplemented with 0.5% human serum albumin (HSA) in a volume between 5.8 to 8.4 mL. Characteristics of TRCs from patients in the RESTORE-CLI interim analysis are presented in Results.
- the primary endpoint of the study was safety, which included adverse events, aspiration site assessment, injection toxicities, and injection site assessments. Amputation rates and wound healing, while also safety endpoints, are described below under Efficacy Evaluations.
- TRC treatment was assessed by secondary endpoints. Principal efficacy measures included time to first occurrence of treatment failure, amputation-free survival, incidence of major amputation, and wound healing. Study investigators made amputation decisions independently based on their clinical judgment.
- the composite treatment failure endpoint was comprised of the following events: major amputation on the treated/injected limb, death, doubling of wound total surface area from baseline (day 0) or occurrence of de novo gangrene.
- Major amputation was defined as amputation at or above the talus on the limb receiving injections.
- the time to first occurrence of treatment failure was defined as the earliest day at which any one of the treatment failure events occurred. For patients who did not experience any of the treatment failure events, their last day in the study was used to calculate event-free duration.
- the duration of amputation-free survival was defined as the first day on which a major amputation or death was reported. For patients who did not experience a major amputation or death, their last day in the study was used to determine the event-free duration.
- TTF time to treatment failure
- AFS amputation free survival
- Amputation free-survival and time to first occurrence of treatment failure were both summarized using Kaplan-Meier plots by treatment group; the p-value from the log-rank test was provided for descriptive purposes.
- Major amputation rates at 6 and 12 months were analyzed using Fisher's exact test.
- TRC tissue repair cells
- the cell surface phenotype of cells from both the bone marrow aspirates and the TRC product was assessed by flow cytometry in 19 of the patients that received TRCs.
- the results, presented in Figure 3, are consistent with established phenotypic differences between unprocessed bone marrow mononuclear cells and culture expanded TRCs (Dennis et al. Stem Cells. 2007 Oct; 25(10): 2575-82).
- the total number of cells was decreased by more than half primarily due loss of non-proliferative hematopoietic cells, including mature lymphocytes and granulocytes, which is reflected in the marked decrease in the number CD45 + cells.
- CD90 + mesenchymal cell population was expanded about 25-fold from 1 to 25 million cells.
- AEs overall adverse events
- Table 13 A summary of overall adverse events (AEs) is shown in Table 13. Nearly all patients reported AEs; the proportion of AEs in TRC-treated and control groups was consistent with the 32 to 14 patient randomization. The percentage of patients with serious adverse events (SAEs) was similar between the groups: 44% in TRC-treated and 57% in control patients. There was one death each in the TRC-treatment and in the control groups; neither was considered related to treatment. AEs reported by a total of 4 or more patients in the 6-month population are listed in Table 14. Bone marrow aspiration and injection site toxicities were minimal.
- Table 15 provides an overall summary of safety for the Safety Population (aspirated patients) at the end of the study.
- In the ixmyelocel-T group three of the patients who died were still participating in the study at the time of their death (one patient died on Study Day 148 related to a hip fracture; one patient died on Study Day 132 related to congestive cardiac failure; and one patient died on Study Day 333 related to renal impairment). The final ixmyelocel-T patient who died had completed the study, but later died of glioblastoma on post-Study Day 498.
- In the Control group both patients who died were still participating in the study at the time of their death (one patient died of hypovolemic shock on Study Day 37; and one patient died of cardiac disorder on Study Day 258).
- TRC tissue repair cells
- Treatment failure was defined as a composite endpoint of major amputation, death, doubling of wound size from baseline or de novo occurrence of gangrene.
- RESTORE-CLI is the first placebo-controlled autologous stem cell trial to use expanded bone marrow mononuclear cells (TRCs) to treat no-option CLI patients. It is double-blinded and studies a larger subject population than previously reported cellular therapy studies in CLI. Interim analysis of this phase II trial has demonstrated that TRC therapy is safe and yields potential improvement in efficacy outcomes.
- TRCs expanded bone marrow mononuclear cells
- Advantages of the cell expansion technique reported here include the need to collect a relatively small amount of bone marrow under local anesthesia. Alternative techniques require harvesting up to 500-600 mL of bone marrow under general anesthesia. Other potential advantages of TRCs are that the expansion process enriches for the cell lineages thought to be important for angiogenesis and neovascularization and may reverse the suppressive effects of chronic medical conditions on bone marrow progenitors that may impair their regenerative function (Lawall H. et al. Thromb Haemost. 2010 Mar 31 ; 103(4): 696-709).
- TRCs intra-muscular injection of bone marrow- derived TRCs is safe and well tolerated, and provides a significant improvement in amputation- free survival and time to first occurrence of treatment failure when compared to control subjects.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Cell Biology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Epidemiology (AREA)
- Vascular Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Urology & Nephrology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2802203A CA2802203A1 (en) | 2010-06-10 | 2011-06-10 | Compositions and methods of treating no-option critical limb ischemia (cli) |
JP2013514402A JP2013528230A (en) | 2010-06-10 | 2011-06-10 | Compositions and methods for treating no option severe ischemic limb (CLI) |
AU2011265194A AU2011265194A1 (en) | 2010-06-10 | 2011-06-10 | Compositions and methods of treating no-option critical limb ischemia (CLI) |
EP11726304.6A EP2579882A1 (en) | 2010-06-10 | 2011-06-10 | Compositions and methods of treating no-option critical limb ischemia (cli) |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35351210P | 2010-06-10 | 2010-06-10 | |
US61/353,512 | 2010-06-10 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2011156784A1 true WO2011156784A1 (en) | 2011-12-15 |
Family
ID=44246418
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2011/040087 WO2011156784A1 (en) | 2010-06-10 | 2011-06-10 | Compositions and methods of treating no-option critical limb ischemia (cli) |
Country Status (6)
Country | Link |
---|---|
US (1) | US20120100108A1 (en) |
EP (1) | EP2579882A1 (en) |
JP (1) | JP2013528230A (en) |
AU (1) | AU2011265194A1 (en) |
CA (1) | CA2802203A1 (en) |
WO (1) | WO2011156784A1 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013142237A1 (en) * | 2012-03-23 | 2013-09-26 | Aastrom Biosciences, Inc. | Cell compositions and methods of using same |
WO2014089397A1 (en) * | 2012-12-06 | 2014-06-12 | Aastrom Biosciences, Inc. | Compositions and methods of treating and preventing pulmonary fibrosis |
WO2014153089A1 (en) * | 2013-03-14 | 2014-09-25 | Aastrom Biosciences, Inc. | Cd14+ cell compositions and methods of using same |
CN107949390A (en) * | 2015-05-01 | 2018-04-20 | 赛斯卡医疗有限公司 | Intramuscular administration for the autologous bone marrow for the treatment of |
US11324779B2 (en) | 2015-03-02 | 2022-05-10 | The Trustees Of Columbia University In The City Of New York | Injectable microtissue systems, devices, and methods |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150064141A1 (en) | 2012-04-05 | 2015-03-05 | The Regents Of The University Of California | Regenerative sera cells and mesenchymal stem cells |
US10331856B1 (en) * | 2012-12-19 | 2019-06-25 | One Call Care Management | Physical therapy patient treatment monitoring systems and methods |
US10426796B2 (en) | 2016-06-13 | 2019-10-01 | SMART SURGICAL, Inc. | Compositions for biological systems and methods for preparing and using the same |
US10456423B2 (en) | 2016-06-13 | 2019-10-29 | SMART SURGICAL, Inc. | Compositions for biological systems and methods for preparing and using the same |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070023302A1 (en) | 2005-07-29 | 2007-02-01 | Nationalpak Limited | Watch box with back-up effective security locker and warranty compartment |
WO2008054825A2 (en) | 2006-11-03 | 2008-05-08 | Aastrom Biosciences, Inc. | Mixed cell populations for tissue repair and separation technique for cell processing |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9598673B2 (en) * | 2006-05-19 | 2017-03-21 | Creative Medical Health | Treatment of disc degenerative disease |
-
2011
- 2011-06-10 WO PCT/US2011/040087 patent/WO2011156784A1/en active Application Filing
- 2011-06-10 EP EP11726304.6A patent/EP2579882A1/en not_active Withdrawn
- 2011-06-10 AU AU2011265194A patent/AU2011265194A1/en not_active Abandoned
- 2011-06-10 US US13/158,298 patent/US20120100108A1/en not_active Abandoned
- 2011-06-10 CA CA2802203A patent/CA2802203A1/en not_active Abandoned
- 2011-06-10 JP JP2013514402A patent/JP2013528230A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070023302A1 (en) | 2005-07-29 | 2007-02-01 | Nationalpak Limited | Watch box with back-up effective security locker and warranty compartment |
WO2008054825A2 (en) | 2006-11-03 | 2008-05-08 | Aastrom Biosciences, Inc. | Mixed cell populations for tissue repair and separation technique for cell processing |
Non-Patent Citations (38)
Title |
---|
AGGARWAL S., PITTENGER M.F., BLOOD, vol. 105, 2005, pages 1815 - 1822 |
ANON: "Aastrom Reports Interim Results from Critical Limb Ischemia Trial", 24 February 2010 (2010-02-24), Ann Arbor, USA, XP002650611, Retrieved from the Internet <URL:http://files.shareholder.com/downloads/ASTM/1322001252x0x352386/944cb334-7267-42d5-9af3-9b6fefdee6ae/ASTMD_News_2010_2_24_Financial.pdf> [retrieved on 20110715] * |
ANON: "Interim Results from Aastrom Critical Limb Ischemia Study Presented Today at Society for Vascular Surgery Annual Meeting", 11 June 2010 (2010-06-11), Ann Arbor, USA, XP002650613, Retrieved from the Internet <URL:http://files.shareholder.com/downloads/ASTM/1322001252x0x381412/c1d48551-7f3a-42c4-ad9b-1de598263872/ASTM_News_2010_6_11_Financial.pdf> [retrieved on 20110715] * |
ANON: "Use of Vascular Repair Cells (VRC) in Patients with Peripheral Arterial Disease to Treat Critical Limb Ischemia (RESTORE-CLI)", 30 April 2007 (2007-04-30), ClinicalTrials.gov, XP002650614, Retrieved from the Internet <URL:http://clinicaltrials.gov/ct2/show/study/NCT00468000> [retrieved on 20110715] * |
DEANS RJ, MOSELEY AB, EXP. HEMATOL., vol. 28, 2000, pages 875 - 884 |
DENNIS ET AL., STEM CELLS, vol. 25, no. 10, October 2007 (2007-10-01), pages 2575 - 82 |
DEVINE SM, J CELL BIOCHEM SUPP, vol. 38, 2002, pages 73 - 79 |
DUFFIELD J.S., CLIN SCI, vol. 104, 2003, pages 27 - 38 |
ERMANN J. ET AL., BLOOD, vol. 105, 2005, pages 2220 - 2226 |
FANDRICH F ET AL., HUM. IMMUNOL., vol. 63, 2002, pages 805 - 812 |
GORDON, S., NAT. REV. IMMUNOL., vol. 3, 2003, pages 23 - 35 |
GRACA L. ET AL., J. IMMUNOL., vol. 168, 2002, pages 5558 - 5565 |
GRONTHOS S ET AL., J CELL PHYSIOL, vol. 189, 2001, pages 54 - 63 |
HOFFMANN P. ET AL., CURR. TOP. MICROBIOL. IMMUNOL., vol. 293, 2005, pages 265 - 285 |
KATZ AJ ET AL., STEM CELLS, vol. 23, 2005, pages 412 - 423 |
KINGSLEY C.I. ET AL., J. IMMUNOL., vol. 168, 2002, pages 1080 - 1086 |
KRAMPERA M. ET AL., STEM CELLS, 2005 |
LAWALL H. ET AL., THROMB HAEMOST., vol. 103, no. 4, 31 March 2010 (2010-03-31), pages 696 - 709 |
LUNDELL ET AL.: "Clinical Scale Expansion of Cryopreserved Small Volume Whole Bone Marrow Aspirates Produces Sufficient Cells for Clinical Use", J. HEMATOTHERAPY, vol. 8, 1999, pages 115 - 127 |
MEISEL R. ET AL., BLOOD, vol. 103, 2004, pages 4619 - 4621 |
MEISEL R., BLOOD, vol. 103, 2004, pages 4619 - 4621 |
MELLOR A.L., MUNN D.H., J. IMMUNOL., vol. 170, 2003, pages 5809 - 5813 |
MOSSCR, D.M., J. LCUKOC. BIOL., vol. 73, 2003, pages 209 - 212 |
MUNN D H. ET AL., J. IMMUNOL., vol. 156, 1996, pages 523 - 532 |
MUNN D.H. ET AL., J EXP MED., vol. 189, 1999, pages 1363 - 1372 |
MUNN D.H. ET AL., J. LMMUNOL., vol. 156, 1996, pages 523 - 532 |
MUNN D.H., MELLOR A.L., CURR PHARM DES., vol. 9, 2003, pages 257 - 264 |
MUNN D.I1., MELLOR A.L., CURR. PHARM. DES., vol. 9, 2003, pages 257 - 264 |
MUNN D.IL. ET AL., J. EXP. MED., vol. 189, 1999, pages 1363 - 1372 |
MURPHY T.J. ET AL., J. IMMUNOL., vol. 174, 2005, pages 2957 - 2963 |
PHILIPPIDIS P. ET AL., CIRC. RCS., vol. 94, 2004, pages 119 - 126 |
POWELL ET AL.: "Interim Results from a Randomized, Placebo Controlled, Double-Blind Multi-Center Phase II Trial Comparing Expanded Autologous Bone Marrow Treatment in Patients with Critical Limb Ischemia", 11 July 2010 (2010-07-11), XP002650612, Retrieved from the Internet <URL:http://files.shareholder.com/downloads/ASTM/1322996334x0x422063/28e15764-64b4-40ac-8d32-d39de10ada4b/SVS_presentation_final.pdf> [retrieved on 20110715] * |
SAKAGUCHI S. ET AL., IMMUNOL. REV., vol. 182, 2001, pages 18 - 32 |
See also references of EP2579882A1 |
SPRENGERS RALF W ET AL: "Progenitor cell therapy in patients with critical limb ischemia without surgical options", ANNALS OF SURGERY, J.B. LIPPINCOTT COMPANY, PHILADELPHIA, US, vol. 247, no. 3, 1 March 2008 (2008-03-01), pages 411 - 420, XP009130888, ISSN: 0003-4932 * |
TANG Q. ET AL., J. EXP. MED., vol. 199, 2004, pages 1455 - 1465 |
TAYLOR P.A. ET AL., BLOOD, vol. 104, 2004, pages 3804 - 3812 |
ZUK PA ET AL., MOL BIOL CELL, vol. 13, 2002, pages 4279 - 95 |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013142237A1 (en) * | 2012-03-23 | 2013-09-26 | Aastrom Biosciences, Inc. | Cell compositions and methods of using same |
WO2014089397A1 (en) * | 2012-12-06 | 2014-06-12 | Aastrom Biosciences, Inc. | Compositions and methods of treating and preventing pulmonary fibrosis |
WO2014153089A1 (en) * | 2013-03-14 | 2014-09-25 | Aastrom Biosciences, Inc. | Cd14+ cell compositions and methods of using same |
US11324779B2 (en) | 2015-03-02 | 2022-05-10 | The Trustees Of Columbia University In The City Of New York | Injectable microtissue systems, devices, and methods |
CN107949390A (en) * | 2015-05-01 | 2018-04-20 | 赛斯卡医疗有限公司 | Intramuscular administration for the autologous bone marrow for the treatment of |
EP3288566A4 (en) * | 2015-05-01 | 2018-11-14 | Cesca Therapeutics Inc. | Intramuscular administration of autologous bone marrow for treatment |
Also Published As
Publication number | Publication date |
---|---|
EP2579882A1 (en) | 2013-04-17 |
JP2013528230A (en) | 2013-07-08 |
US20120100108A1 (en) | 2012-04-26 |
AU2011265194A1 (en) | 2013-01-10 |
CA2802203A1 (en) | 2011-12-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20120100108A1 (en) | Compositions and methods of treating no-option critical limb ischemia (cli) | |
Ge et al. | Infusion of mesenchymal stem cells and rapamycin synergize to attenuate alloimmune responses and promote cardiac allograft tolerance | |
US20190276803A1 (en) | Method of culturing immune cells, kit for thereof, immune cell cultured medium obtained by same method, cosmetic composition and pharmaceutical composition comprising thereof | |
KR101614823B1 (en) | Cell growth method and pharmaceutical preparation for tissue repair and regeneration | |
US20120201791A1 (en) | Methods of treating diseases or conditions using mesenchymal stem cells | |
JP2011529706A (en) | Mesenchymal stromal cell population and methods of isolating and using the same | |
JP6622189B2 (en) | Method for using adipose tissue-derived cells in the regulation of pain and / or fibrosis | |
US20200030380A1 (en) | Stimulation of therapeutic angiogenesis by t regulatory cells | |
US11738046B2 (en) | Macrophage cell therapy to treat orthopedic injury | |
JP2015159895A (en) | Pluripotent stem cell for cerebral infarction treatment | |
WO2009114860A2 (en) | Activated mesenchymal stem cells for the prevention and repair of inflammatory states | |
Gao et al. | The immunosuppressive properties of non-cultured dermal-derived mesenchymal stromal cells and the control of graft-versus-host disease | |
AU2017202192A1 (en) | Mesenchymal Stromal Cell Populations and Methods of Making Same | |
JP2001515505A (en) | Mononuclear phagocytic cells promoting axonal regeneration and uses thereof | |
AU2012255112A1 (en) | Mesenchymal stromal cell populations and methods of making same | |
WO2014089397A1 (en) | Compositions and methods of treating and preventing pulmonary fibrosis | |
KR20080048463A (en) | Method for regenerating an immune system | |
WO2021131261A1 (en) | Cell group and method for acquiring same | |
JP2018111722A (en) | Pluripotent stem cells for treatment of cerebral infarction | |
US20220280563A1 (en) | Therapeutic apoptotic cells for treatment of osteoarthritis | |
Bērziņš et al. | Characterisation and Safety of Canine Adipose-Derived Stem Cells | |
US20210154230A1 (en) | Intradiscal t-regulatory cell administration for treatment of disc degenerative disease | |
JP2021531279A (en) | Suppression of microglial activation by natural lymphoid cells | |
WO2024133886A1 (en) | Primed mesenchymal stem cells for use in the treatment of chronic kidney disease | |
Pittenger et al. | Immunological properties of mesenchymal stem cells isolated from bone marrow and umbilical cord |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 11726304 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2013514402 Country of ref document: JP Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 2802203 Country of ref document: CA |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2011726304 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref document number: 2011265194 Country of ref document: AU Date of ref document: 20110610 Kind code of ref document: A |