WO2011156073A1 - Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts - Google Patents

Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts Download PDF

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Publication number
WO2011156073A1
WO2011156073A1 PCT/US2011/035936 US2011035936W WO2011156073A1 WO 2011156073 A1 WO2011156073 A1 WO 2011156073A1 US 2011035936 W US2011035936 W US 2011035936W WO 2011156073 A1 WO2011156073 A1 WO 2011156073A1
Authority
WO
WIPO (PCT)
Prior art keywords
phosphate
protein
interest
method
composite filter
Prior art date
Application number
PCT/US2011/035936
Other languages
English (en)
French (fr)
Inventor
Potty Ajish
Xenopolous Alex
Original Assignee
Millipore Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US39722910P priority Critical
Priority to US61/397,229 priority
Application filed by Millipore Corporation filed Critical Millipore Corporation
Publication of WO2011156073A1 publication Critical patent/WO2011156073A1/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
PCT/US2011/035936 2010-06-08 2011-05-10 Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts WO2011156073A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US39722910P true 2010-06-08 2010-06-08
US61/397,229 2010-06-08

Publications (1)

Publication Number Publication Date
WO2011156073A1 true WO2011156073A1 (en) 2011-12-15

Family

ID=44217341

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/035936 WO2011156073A1 (en) 2010-06-08 2011-05-10 Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts

Country Status (2)

Country Link
US (1) US20110301333A1 (patent-search.search_20190918_RC01)
WO (1) WO2011156073A1 (patent-search.search_20190918_RC01)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
CN110054661A (zh) 2013-12-12 2019-07-26 Emd密理博公司 使用含丙烯酰胺的过滤器分离蛋白

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0313090A2 (en) * 1987-10-22 1989-04-26 Asahi Kogaku Kogyo Kabushiki Kaisha Porous ceramic material
WO2005044856A2 (en) * 2003-10-27 2005-05-19 Wyeth Removal of high molecular weight aggregates using hydroxyapatite chromatography
US20090215997A1 (en) * 2008-02-22 2009-08-27 Tsuneo Okuyama Separation method
WO2010147686A2 (en) * 2009-03-11 2010-12-23 Wyeth Llc Methods of purifying small modular immunopharmaceutical proteins

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0313090A2 (en) * 1987-10-22 1989-04-26 Asahi Kogaku Kogyo Kabushiki Kaisha Porous ceramic material
WO2005044856A2 (en) * 2003-10-27 2005-05-19 Wyeth Removal of high molecular weight aggregates using hydroxyapatite chromatography
US20090215997A1 (en) * 2008-02-22 2009-08-27 Tsuneo Okuyama Separation method
WO2010147686A2 (en) * 2009-03-11 2010-12-23 Wyeth Llc Methods of purifying small modular immunopharmaceutical proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OHTA K. & AL.: "Adsorption characteristics of proteins on calcium phosphates using liquid chromatography", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, vol. 55, no. 3, 5 June 2001 (2001-06-05), pages 409-414, XP000002656355, *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3130384A1 (en) 2012-06-29 2017-02-15 EMD Millipore Corporation Purification of biological molecules
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules

Also Published As

Publication number Publication date
US20110301333A1 (en) 2011-12-08

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