WO2011144742A1 - Novel pyrimidine derivatives - Google Patents

Novel pyrimidine derivatives Download PDF

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Publication number
WO2011144742A1
WO2011144742A1 PCT/EP2011/058271 EP2011058271W WO2011144742A1 WO 2011144742 A1 WO2011144742 A1 WO 2011144742A1 EP 2011058271 W EP2011058271 W EP 2011058271W WO 2011144742 A1 WO2011144742 A1 WO 2011144742A1
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WO
WIPO (PCT)
Prior art keywords
alkyl
indol
aryl
heteroaryl
heterocyclyl
Prior art date
Application number
PCT/EP2011/058271
Other languages
French (fr)
Inventor
Marita Högberg
Tommy Johansson
Emma Dahlstedt
Olof Smitt
Original Assignee
Chemilia Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP10163597A external-priority patent/EP2395001A1/en
Priority to CA2798578A priority Critical patent/CA2798578C/en
Priority to AU2011254550A priority patent/AU2011254550B2/en
Priority to BR112012029647A priority patent/BR112012029647A2/en
Priority to US13/697,954 priority patent/US8927547B2/en
Priority to EP11720532.8A priority patent/EP2571867B1/en
Application filed by Chemilia Ab filed Critical Chemilia Ab
Priority to JP2013510640A priority patent/JP5607241B2/en
Priority to RU2012155712/04A priority patent/RU2528386C2/en
Priority to KR1020127033533A priority patent/KR20130031296A/en
Priority to CN201180034863.5A priority patent/CN103003264B/en
Priority to MX2012013274A priority patent/MX2012013274A/en
Publication of WO2011144742A1 publication Critical patent/WO2011144742A1/en
Priority to IL222867A priority patent/IL222867A0/en
Priority to ZA2012/09029A priority patent/ZA201209029B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5375—1,4-Oxazines, e.g. morpholine
    • A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing three or more hetero rings

Definitions

  • This invention relates to novel pyrimidine derivatives of formula I, to methods of preparing such compounds, to pharmaceutical compositions containing such compounds, and to methods for using such compounds in treatment of diseases including cancer.
  • Cancer is a major and often fatal disease. Accordingly, the development of new therapies for cancer is an ongoing process of outmost importance. The majority of cancers are present as solid tumours, such as lung cancer, breast cancer and prostate cancer, while others represent haemato logical and lymphoid malignancies, such as leukaemias and lymphomas.
  • TTKs Tyrosine Kinase Receptors
  • monoclonal antibodies and tyrosine kinase inhibitors Two classes of compounds targeting RTKs are currently used in clinical practice: monoclonal antibodies and tyrosine kinase inhibitors.
  • the first approved targeted therapies were trastuzumab, a monoclonal antibody against HER2, for treatment of metastatic breast cancer, and imatinib, a small tyrosine kinase inhibitor targeting BCR-Abl, in Chronic Myeloid Leukemia.
  • tubulin Another example of an important target for cancer chemotherapy is tubulin.
  • the targeting drugs in this therapy interrupt microtubule spindle-mediated chromosome segregation, arrest the dividing tumor cells in mitosis and subsequently induce apoptosis.
  • Existing drugs are targeting microtubules via two main mechanisms, e.g. molecules of the taxane class (that stabilize the tubulins) and several vinca alkaloids (destabilizers).
  • novel tubulin polymerization inhibitors see e.g. WO 2009/070645 and US
  • cancer chemotherapy is used in attempts to cure or palliate the disease. In most cases this therapy is delivered in the form of combination chemotherapy, i.e. when two or more drugs having different modes of action are used together in order to optimise the effect on the cancer cells and to minimise side effects.
  • the results obtained with chemotherapy vary according to tumour type. Some tumours are very sensitive and the treatment has a high probability of leading to beneficial treatment results including cure of the disease. Examples of this type of tumours are acute leukaemias, malignant lymphomas, testicular cancer, chorion carcinomas, and Wilms tumour. Other types of cancer chemotherapy can result in effective palliation and prolonged survival.
  • tumours are breast cancer, colorectal cancer, ovarian cancer, small-cell lung cancer, bladder cancer, multiple myeloma, and chronic leukaemias of both the lymphatic and myeloid type.
  • Primary drug resistant tumours which respond poorly to classical chemotherapy include malignant glioma, melanoma, prostate cancer, sarcomas, and gastrointestinal tumours other than colorectal cancers (see e.g. DeVita, Hellman, and Rosenberg: Cancer: Principles & Practice of Oncology, Eighth Edition 978-0-7817-7207-5).
  • Certain pyrimidine compounds and their potential use in the treatment of cancer are disclosed in for example WO2003/030909, WO2003/063794, WO2004/056807, WO2004/056786, US2004/220177, WO2005/013996, WO2006/133426,
  • WO2007/085833 WO2008/128231 and WO2009/063240.
  • What is needed in the art are targeted drugs that work in a specific manner, being selective in eliminating subpopulations of cells involved in tumour survival and progression.
  • the present invention provides novel pyrimidine compounds having a surprisingly efficient and selective antiproliferative activity. Hence, these novel compounds are useful in the treatment of proliferative diseases, such as cancer.
  • the present invention provides compounds of formula I or a pharmaceutically acceptable ester, amide, solvate or salt thereof,
  • Z represents carbon or nitrogen
  • Y represents carbon or nitrogen; wherein one of Z and Y represents nitrogen;
  • A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and D and E represents carbon; or A and D represent nitrogen and E represents carbon; or A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen or methyl, when D represents carbon
  • R 2 represents hydrogen or amino, when Y or Z represents carbon
  • R 3 represents hydrogen, (Ci-C 3 )alkyl, amino, trifluoromethyl or (Co-Ci)alkylaryl;
  • R 4 represents heteroaryl, optionally substituted with one or more substituents; and
  • R 5 represents hydrogen or methyl.
  • Z represents carbon or nitrogen
  • Y represents carbon or nitrogen; wherein one of Z and Y represents nitrogen;
  • A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and D and E represents carbon; or A and D represent nitrogen and E represents carbon; or A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen or methyl, when D represents carbon
  • R 2 represents hydrogen or amino, when Y or Z represents carbon
  • R 3 represents hydrogen, (Ci-C 3 )alkyl, amino, trifluoromethyl or (Co-Ci)alkylaryl;
  • R 4 represents heteroaryl, optionally substituted with one or more substituents; and
  • R 5 represents hydrogen or methyl;
  • R 4 represents heteroaryl that is optionally substituted with one or more substituents.
  • substituents include, but are not limited to, halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (C 1 -C 4 )alkyl(C 2 - C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci- C 4 )alkyl(CO)NH 2 , (Ci-C 4 )alkyl(CO)NH(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl- 0(C 6 -Cio)aryl, (Ci-C 4 )alkyl-0(CO)(Ci-C 4 )alkyl, (Ci-C 4 )alkyl
  • Cio)aryl 0(CO)(Ci-C 4 )alkyl, O(CO)(C 6 -Ci 0 )aryl, OS0 2 OH, NH(Ci-C 4 )alkyl, N[(Ci- C 4 )alkyl][(Ci-C 4 )alkyl], NH(CO)(Ci-C 4 )alkyl, NH(CO)(C 6 -Ci 0 )aryl, and CF 3 .
  • R 4 represents heteroaryl that is optionally substituted with one or more substituents.
  • substituents include, but are not limited to, halogen, hydroxy, amino, nitro, cyano, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl(C 2 - C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci- C 4 )alkyl(CO)NH 2 , (Ci-C 4 )alkyl(CO)NH(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO) H(Ci- C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl-0(Ci-C 4 )alkyl, (d-
  • R 4 represents heteroaryl that is optionally substituted with one or more substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH 2 , (Ci- C 4 )alkyl(CO)NH(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH(Ci-C 4 )alkyl(CO)OH, (Ci- C 4 )alkyl-OH, (Ci-C 4 )alkyl-0(Ci-C 4 )alkyl, (Ci-C 4 )al
  • L represents a bond
  • L represents Ci-alkyl (methylene).
  • L represents C 2 -alkyl (ethylene).
  • R 6 is selected from hydrogen and (Ci-C 4 )alkyl
  • R 7 is selected from hydrogen, halogen, nitro, cyano, (Ci-C 4 )alkyl, (Ci- C 4 )alkyl(CO)OH, (Ci-C )alkyl(CO)0(Ci-C 4 )alkyl, (Ci-C )alkyl(CO)NH 2 , (d- C 4 )alkyl(CO)NH(Ci-C 4 )alkyl, (C]-C 4 )alkyl-OH, (Ci-C 4 )alkyl-O(C 6 -Ci 0 )aryl, (d- C 4 )alkyl-0(CO)(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-O(CO)(C 6 -Ci 0 )aryl, (Ci-C 4 )alkyl-NH 2 , (Ci-C 4 )alkyl-NH(Ci-C 4 )alkyl,
  • R 8 is selected from hydrogen, halogen, nitro, cyano, hydroxy, amino, (Ci-C 4 )alkyl, (C i -C 4 )alky l(C 2 -C 9 )heterocyclyl, (C i -C 4 )alkyl-NH 2 , (C i -C 4 )alky 1-NH(C i -C 4 )alkyl, (Ci-C 4 )alkyl-N[(Ci-C4)alkyl][(Ci-C 4 )-alkyl], (Ci-C 4 )alkyl-NH(CO)(Ci-C 4 )alkyl, (Ci- C 4 )alkyl-NH(CO)(C 6 -Cio)aryl, (CO)(Ci-C 4 )alkyl, (CO)OH, (CO)0(Ci-C 4 )alkyl, (CO)NH 2 , (CO)NH(C
  • R 9 is selected from hydrogen, halogen, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl- 0(Ci-C 4 )alkyl, (CO)OH, (CO)0(Ci-C 4 )alkyl, (CO)NH 2 , (CO)NH(Ci-C 4 )alkyl, and
  • R 10 is selected from hydrogen, halogen, hydroxy, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl-OH, (Ci- C 4 )alkyl-0(Ci-C 4 )alkyl, and 0(Ci-C 4 )alkyl; and
  • R 1 1 is selected from hydrogen, hydroxy, (Ci-C 4 )alkyl, and 0(Ci-C 4 )alkyl.
  • R 6 is selected from hydrogen and (Ci-C 4 )alkyl
  • R 7 is selected from hydrogen, halogen, nitro, cyano, (Ci-C 4 )alkyl, (Ci- C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH 2 , (d- C 4 )alkyl(CO)NH(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl-0(C 6 -Cio)aryl, (Ci- C 4 )alkyl-0(CO)(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-0(CO)(C 6 -Cio)aryl, (Ci-C 4 )alkyl-NH 2 , (Ci-C 4 )alkyl-NH(Ci-C 4 )alkyl, (
  • R 8 is selected from hydrogen, halogen, nitro, cyano, hydroxy, amino, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl-NH 2 , (Ci-C 4 )alkyl-NH(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-N[(Ci-C 4 )alkyl][(Ci-C 4 )-alkyl], (Ci-C 4 )alkyl-NH(CO)(Ci-C 4 )alkyl, (Ci- C 4 )alkyl-NH(CO)(C 6 -Cio)aryl, (CO)(Ci-C 4 )alkyl, (CO)OH, (CO)0(Ci-C 4 )alkyl, (CO)NH 2 , (CO)NH(Ci-C 4 )
  • R 9 is selected from hydrogen, halogen, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl- 0(Ci-C 4 )alkyl, (CO)OH, (CO)0(Ci-C 4 )alkyl, (CO)NH 2 , (CO)NH(Ci-C 4 )alkyl, and (C 6 -C 10 )aryl;
  • R 10 is selected from hydrogen, halogen, hydroxy, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl-OH, (Ci- C 4 )alkyl-0(Ci-C 4 )alkyl, and 0(Ci-C 4 )alkyl; and
  • R 1 1 is selected from hydrogen, hydroxy, (Ci-C 4 )alkyl, and 0(Ci-C 4 )alkyl.
  • R 6 represents hydrogen
  • R 7 is selected from hydrogen and (Ci-C4)alkyl, preferably methyl;
  • R 8 , R 9 and R 10 represents hydrogen
  • R 11 is selected from hydrogen, hydroxy, (Ci-C4)alkyl, preferably methyl, and 0(Ci- C4)alkyl, preferably methoxy.
  • R 6 is selected from hydrogen and (Ci-C4)alkyl
  • R 7 is selected from hydrogen, halogen, nitro, cyano, (Ci-C4)alkyl, (Ci- C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH 2 , (d- C 4 )alkyl(CO)NH(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl-0(C 6 -Cio)aryl, (Ci- C 4 )alkyl-0(CO)(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-0(CO)(C 6 -Cio)aryl, (Ci-C 4 )alkyl-NH 2 , (Ci-C 4 )alkyl-NH(Ci-C 4 )alkyl, (Ci
  • R 7 a and R 7 b are independently selected from hydrogen and (Ci-C 4 )alkyl, preferably methyl;
  • R 8 is selected from hydrogen, halogen, nitro, cyano, hydroxy, amino, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl-NH 2 , (Ci-C 4 )alkyl-NH(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-N[(Ci-C 4 )alkyl][(Ci-C 4 )-alkyl], (Ci-C 4 )alkyl-NH(CO)(Ci-C 4 )alkyl, (Ci- C 4 )alkyl-NH(CO)(C 6 -Cio)aryl, (CO)(Ci-C 4 )alkyl, (CO)OH, (CO)0(Ci-C 4 )alkyl, (CO)NH 2 , (CO)NH(Ci-C 4 )
  • R 9 is selected from hydrogen, halogen, (Ci-C 4 )alkyl, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl- 0(Ci-C 4 )alkyl, (CO)OH, (CO)0(Ci-C 4 )alkyl, (CO)NH 2 , (CO)NH(Ci-C 4 )alkyl, and
  • R 1 1 is selected from hydrogen, hydroxy, (Ci-C 4 )alkyl, and 0(Ci-C 4 )alkyl.
  • R 6 and R 8 represent hydrogen
  • R 7 a and R 7 b are independently selected from hydrogen and (Ci-C4)alkyl, preferably methyl.
  • R 5 represents hydrogen.
  • Y and A represent nitrogen
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen or methyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci- C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH 2 , (Ci-C 4 )alkyl(CO) H(Ci- C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH(Ci-C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl-OH, (Ci-C 4
  • R 5 represents hydrogen or methyl.
  • Y, D, and A represent nitrogen
  • L represents a bond or (Ci-C 2 )alkyl
  • R 2 represents hydrogen;
  • R 3 represents hydrogen or methyl;
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci-
  • C 9 heterocyclyl(Ci-C 4 )alkyl-NH 2 , 0(Ci-C 4 )alkyl, O(Ci-C 4 )alkyl(C 6 -Ci 0 )aryl, 0(Ci- C 4 )alkyl(C 6 -Cio)heteroaryl, 0(Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyL 0(Ci-C 4 )alkyl(C 2 - C 9 )heterocyclyl(Ci-C 4 )alkyl, 0(Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl(Ci-C 4 )alkyl-OH, 0(EtO)i_ 3 H, 0(EtO)i_ 3 (Ci-C 4 )alkyl, O(C 6 -Ci 0 )aryl, 0
  • C 4 )alkyl N[(Ci-C 4 )alkyl][(Ci-C 4 )alkyl], NH(CO)(Ci-C 4 )alkyl, NH(CO)(Ci-C 4 )alkyl- NH 2 , NH(CO)(C 6 -Cio)aryl, NHS0 2 (Ci-C 4 )alkyl, S0 2 NH 2 , and CF 3 ; and
  • R 5 represents hydrogen or methyl.
  • Y, E and A represent nitrogen
  • L represents a bond or (Ci-C2)alkyl
  • R 1 represents hydrogen
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci-
  • C 9 heterocyclyl(Ci-C 4 )alkyl-NH 2 , 0(Ci-C 4 )alkyl, O(Ci-C 4 )alkyl(C 6 -Ci 0 )aryl, 0(Ci- C 4 )alkyl(C 6 -Cio)heteroaryl, 0(Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyL 0(Ci-C 4 )alkyl(C 2 - C 9 )heterocyclyl(Ci-C 4 )alkyl, 0(Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl(Ci-C 4 )alkyl-OH, 0(EtO)i_ 3 H, 0(EtO)i_ 3 (Ci-C 4 )alkyl, O(C 6 -Ci 0 )aryl, 0
  • R 5 represents hydrogen or methyl.
  • Y and E represent nitrogen
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen or methyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci-
  • R 5 represents hydrogen or methyl.
  • Y, D and E represent carbon
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen or methyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci-
  • R 5 represents hydrogen or methyl.
  • Y and E represent carbon
  • L represents a bond or (Ci-C 2 )alkyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci- C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH 2 , (Ci-C 4 )alkyl(CO) H(Ci-
  • C 4 )alkyl (Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH(Ci-C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl- 0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-O(C 6 -Ci 0 )aryl, (Ci-C 4 )alkyl-0(CO)(Ci-C 4 )alkyl, (Ci- C 4 )alkyl-0(CO)(Ci-C 4 )alkyl-NH 2 , (Ci-C 4 )alkyl-O(CO)(C 6 -Ci 0 )aryl, (Ci-C 4 )alkyl- NH 2 , (Ci-C 4 )alkyl-NH(Ci-C 4 )alkyl, (Ci-C
  • R 5 represents hydrogen or methyl.
  • Y and D represent carbon
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci- C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH 2 , (Ci-C 4 )alkyl(CO)NH(Ci-
  • C 4 )alkyl (Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH(Ci-C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl- 0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-0(C 6 -Cio)aryl, (Ci-C 4 )alkyl-0(CO)(Ci-C 4 )alkyl, (Ci- C 4 )alkyl-0(CO)(Ci-C 4 )alkyl-NH 2 , (Ci-C 4 )alkyl-O(CO)(C 6 -Ci 0 )aryl, (Ci-C 4 )alkyl- NH 2 , (Ci-C 4 )alkyl-NH(Ci-C 4 )alkyl, (Ci-C 4
  • R 5 represents hydrogen or methyl.
  • Y, A and D represent carbon
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C 4 )alkyl, (Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, (Ci-C 4 )alkyl(CO)OH, (Ci- C 4 )alkyl(CO)0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH 2 , (Ci-C 4 )alkyl(CO)NH(Ci-
  • C 4 )alkyl (Ci-C 4 )alkyl, (Ci-C 4 )alkyl(CO)NH(Ci-C 4 )alkyl(CO)OH, (Ci-C 4 )alkyl-OH, (Ci-C 4 )alkyl- 0(Ci-C 4 )alkyl, (Ci-C 4 )alkyl-O(C 6 -Ci 0 )aryl, (Ci-C 4 )alkyl-0(CO)(Ci-C 4 )alkyl, (Ci- C 4 )alkyl-0(CO)(Ci-C 4 )alkyl-NH 2 , (Ci-C 4 )alkyl-O(CO)(C 6 -Ci 0 )aryl, (Ci-C 4 )alkyl- NH 2 , (Ci-C 4 )alkyl-NH(Ci-C 4 )alkyl, (Ci-C
  • R 5 represents hydrogen or methyl.
  • Z, D and E represent carbon; Y and A represent nitrogen;
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen or methyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy;
  • R 5 represents hydrogen or methyl.
  • Y, D, and A represent nitrogen
  • L represents a bond or (Ci-C 2 )alkyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy;
  • R 5 represents hydrogen or methyl.
  • Y, E and A represent nitrogen
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy;
  • R 5 represents hydrogen or methyl.
  • Y and E represent nitrogen
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen or methyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy;
  • R 5 represents hydrogen or methyl.
  • Y, D and E represent carbon
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen or methyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy;
  • R 5 represents hydrogen or methyl.
  • L represents a bond or (Ci-C 2 )alkyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy;
  • R 5 represents hydrogen or methyl.
  • Y and D represent carbon
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy;
  • R 5 represents hydrogen or methyl.
  • Y, A and D represent carbon
  • L represents a bond or (Ci-C 2 )alkyl
  • R 1 represents hydrogen
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • R 4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy;
  • R 5 represents hydrogen or methyl.
  • Y and A represent nitrogen
  • R 1 represents hydrogen or methyl
  • R 2 represents hydrogen
  • R 3 represents hydrogen or methyl
  • L-R 4 is selected from:
  • R 7 represents hydrogen or methyl
  • R 6 represents hydrogen or methyl
  • R 8 is selected from hydrogen, methyl, fluoro, methoxy, ethoxy and OCF 3
  • R 11 is selected from hydrogen, methyl, and methoxy
  • R 5 represents hydrogen or methyl.
  • Y, D and E represent carbon
  • R 1 , R 2 , and R 3 represent hydrogen
  • L-R 4 is selected from:
  • R 6 represents hydrogen or methyl
  • R 8 represents hydrogen
  • R 7 is selected from hydrogen, methyl, (Ci-C4)alkyl-OH, and COOCH 3 ;
  • R 7 a and R 7 b is independently selected from hydrogen and methyl
  • R 5 represents hydrogen or methyl.
  • Z represents carbon or nitrogen
  • Y represents carbon or nitrogen, wherein one of Z and Y represents nitrogen;
  • A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and D and E represents carbon; or A and D represent nitrogen and E represents carbon; or A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
  • R 1 represents hydrogen or methyl, when D represents carbon
  • R 2 represents hydrogen or amino
  • R 3 represents hydrogen, methyl, trifluoromethyl or (Co-Ci)alkylaryl
  • R 5 represents hydrogen or methyl
  • L-R 4 is selected from:
  • R 6 is selected from hydrogen and methyl
  • R 7 is selected from hydrogen, methyl, (Ci-C 4 )alkyl-OH and (CO)OCH 3 ;
  • R 7 a and R 7 b are independently selected from hydrogen, and methyl;
  • R 8 is selected from halogen, hydrogen, hydroxy, (CO)OH, (CO)OCH 3 , 0(Ci-C 4 )alkyl, 0(Ci-C 4 )alkyl(C 6 -Cio)aryl, 0(Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, 0(EtO)i_ 3 (Ci-C 4 )alkyl, and OCF 3 ;
  • R 9 and R 10 represent hydrogen
  • R 11 is selected from hydrogen, methyl, and 0(Ci-C 4 )alkyl.
  • Y represents carbon or nitrogen, wherein one of Z and Y represents nitrogen;
  • A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and
  • D and E represents carbon; or A and D represent nitrogen and E represents carbon; or
  • a and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
  • R 1 represents hydrogen or methyl, when D represents carbon
  • R 2 represents hydrogen or amino
  • R 3 represents hydrogen, methyl, trifluoromethyl or (Co-Ci)alkylaryl
  • R 5 represents hydrogen or methyl
  • L-R 4 is selected from:
  • R 6 is selected from hydrogen and methyl
  • R 7 is selected from hydrogen, methyl, (Ci-C 4 )alkyl-OH, and (CO)OCH 3 ,
  • R 7 a and R 7 b are independently selected from hydrogen, and methyl;
  • R 8 is selected from halogen, hydrogen, hydroxy, (CO)OH, (CO)OCH 3 , 0(Ci-C 4 )alkyl, 0(Ci-C 4 )alkyl(C 6 -Cio)aryl, 0(Ci-C 4 )alkyl(C 2 -C 9 )heterocyclyl, 0(EtO)i_ 3 (Ci-C 4 )alkyl, and OCF 3 ;and
  • R 1 1 is selected from hydrogen, methyl, and 0(Ci-C 4 )alkyl.
  • a compound of formula I in the manufacture of a medicament and pharmaceutical compositions for treatment of cancer.
  • a pharmaceutical composition comprising a compound of formula I, together with pharmaceutically acceptable diluents and carriers.
  • a method for treatment of cancer which comprises administering to a subject in need thereof, a therapeutically effective amount of a compound of formula I.
  • a method for treatment of cancer which comprises administering to a subject in need thereof, a therapeutically effective amount of a compound of formula I, in combination with another compound of formula I, in combination with radiation therapy, or in combination with another anticancer agent selected from alkylating agents, antimetabolites, anticancer camptothecin derivatives, plan-derived anticancer agents, antibiotics, enzymes, platinum coordination complexes, tyrosine kinase inhibitors, hormones, hormone antagonists, monoclonal antibodies, interferons, and biological response modifiers.
  • another anticancer agent selected from alkylating agents, antimetabolites, anticancer camptothecin derivatives, plan-derived anticancer agents, antibiotics, enzymes, platinum coordination complexes, tyrosine kinase inhibitors, hormones, hormone antagonists, monoclonal antibodies, interferons, and biological response modifiers.
  • the compounds may form esters, amides and/or salts which are within the scope of the present invention.
  • Salts and solvates of compounds of formula I which are suitable for use in medicine are those wherein a counterion or an associated solvent is pharmaceutically acceptable.
  • salts and solvates having non- pharmaceutically acceptable counterions or associated solvents are within the scope of the present invention, for example, for use as intermediates in the preparation of the compounds of formula I and their pharmaceutically acceptable salts, solvates and physiologically functional derivatives.
  • physiologically functional derivative is meant a chemical derivative of a compound of formula I having the same physiological function as the free compound of formula I, for example, by being convertible in the body thereto.
  • Esters and amides are examples of physiologically functional derivatives.
  • a compound which, upon administration to the recipient, is capable of being converted into a compound of formula I as described above, or an active metabolite or residue thereof, is known as a "prodrug".
  • a prodrug may, for example, be converted within the body, e. g. by hydrolysis in the blood, into its active form that has medical effects.
  • Pharmaceutical acceptable prodrugs are described in T. Higuchi and V. Stella, Prodrugs as Novel Delivery Systems, Vol. 14 of the A. C. S.
  • Suitable salts according to the invention include those formed with organic or inorganic acids or bases.
  • suitable salts formed with acids according to the invention include those formed with mineral acids, strong organic carboxylic acids, such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted, for example, by halogen, such as saturated or unsaturated dicarboxylic acids, such as hydroxycarboxyhc acids, such as amino acids, or with organic sulfonic acids, such as (C j -C 4 )alkyl- or aryl-sulfonic acids which are unsubstituted or substituted, for example by halogen.
  • mineral acids such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted, for example, by halogen, such as saturated or unsaturated dicarboxylic acids, such as hydroxycarboxyhc acids, such as amino acids, or with organic sulfonic acids, such as (C j -C 4 )alkyl- or aryl-sulfonic
  • Pharmaceutically acceptable acid addition salts include those formed from hydrochloric, hydrobromic, sulphuric, nitric, citric, tartaric, acetic, phosphoric, lactic, pyruvic, acetic, trifluoroacetic, succinic, perchloric, fumaric, maleic, glycolic, lactic, salicylic, oxaloacetic, methanesulfonic,
  • ethanesulfonic p-toluenesulfonic, formic, benzoic, malonic, naphthalene-2-sulfonic, benzenesulfonic, isethionic, ascorbic, malic, phthalic, aspartic, and glutamic acids, lysine and arginine.
  • Other acids such as oxalic, while not in themselves
  • Pharmaceutically acceptable may be useful as intermediates in obtaining the compounds of the invention and their pharmaceutical acceptable acid addition salts.
  • Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts, for example those of potassium and sodium, alkaline earth metal salts, for example those of calcium and magnesium, and salts with organic bases, for example
  • dicyclohexylamine N-methyl-D-glucamine, morpholine, thiomorpholine, piperidine, pyrrolidine, a mono-, di- or tri-lower alkylamine, for example ethyl-, tert-butyl-, diethyl-, diisopropyl-, triethyl-, tributyl- or dimethyl-propylamine, or a mono-, di- or trihydroxy lower alkylamine, for example mono-, di- or triethanolamine.
  • Corresponding internal salts may furthermore be formed.
  • Those skilled in the art of organic chemistry will appreciate that many organic compounds can form complexes with solvents in which they are reacted or from which they are precipitated or crystallized. These complexes are known as
  • alkyl means both straight and branched chain saturated hydrocarbon groups.
  • alkyl groups include methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, and t-butyl groups.
  • unbranched alkyl groups there are preferred methyl, ethyl, n-propyl, and n-butyl groups.
  • branched alkyl groups there may be mentioned iso-propyl, iso-butyl, sec-butyl, and t-butyl groups.
  • alkoxy means the group O-alkyl, where "alkyl” is used as described above.
  • alkoxy groups include, but are not limited to, methoxy and ethoxy groups.
  • Other examples include propoxy and butoxy.
  • aryl means a monocyclic or bicyclic aromatic carbocyclic group.
  • aryl groups include phenyl and naphthyl. A naphthyl group may be attached through the 1 or the 2 position.
  • one of the rings may, for example, be partially saturated. Examples of such groups include indanyl and tetrahydronaphthyl.
  • (C6-Cio)aryl is used herein to mean a group comprising from 6 to 10 carbon atoms in a monocyclic or bicyclic aromatic group.
  • a particularly preferred (C 6 -Cio)aryl group is phenyl.
  • halogen means fluorine, chlorine, bromine or iodine. Fluorine, chlorine and bromine are particularly preferred.
  • heteroaryl means an aromatic cyclic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen or sulfur.
  • a heteroaryl group may, for example, be monocyclic, bicyclic or tricyclic.
  • monocyclic heteroaryl groups include, but are not limited to, furanyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, oxadiazolyl, thiadiazolyl, pyridyl, triazolyl, triazinyl, pyridazyl, iso thiazolyl, isoxazolyl, pyrazinyl, pyrazolyl, and pyrimidinyl.
  • bicyclic heteroaryl groups include, but are not limited to, quinoxalinyl, quinazolinyl, pyridopyrazinyl, benzoxazolyl, benzothiophenyl, benzimidazolyl, naphthyridinyl, quinolinyl, benzo furanyl, indolyl, indazolyl, benzothiazolyl, pyridopyrimidinyl, and isoquinolinyl.
  • tricyclic heteroaryl groups include, but are not limited to, carbazole, dibenzofuran, xanthene, and acridine.
  • heterocyclyl means a cyclic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen and sulfur.
  • heterocyclyl groups include, but are not limited to, tetrahydrofuranyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, and dioxanyl.
  • the compounds of the invention may be used in the prophylaxis and treatment as such, or preferably in a form of a pharmaceutical composition. While it is possible for the active ingredient to be administered alone, it is preferable for it to be present in a pharmaceutical formulation or composition. Accordingly, the invention provides a pharmaceutical formulation comprising a compound according to the invention, and a pharmaceutically acceptable diluent, excipient or carrier (collectively referred to herein as "carrier" materials). Pharmaceutical compositions of the invention may take the form of a pharmaceutical formulation as described below. Thus, the present invention relates to a pharmaceutical composition containing at least one compound of Formula I together with conventional excipients.
  • compositions for oral administration include suspensions which can contain, for example, micro crystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer, and sweeteners or flavoring agents such as those known in the art; and immediate release tablets which can contain, for example, micro crystalline cellulose, dicalcium phosphate, starch, magnesium stearate, calcium sulfate, sorbitol, glucose and/or lactose and/or other excipients, binders, extenders, disintegrants, diluents and lubricants such as those known in the art.
  • Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes and the like.
  • Disintegrators include without limitation starch, methylcellulose, agar, bentonite, xanthan gum and the like.
  • the compounds of formula I can also be delivered through the oral cavity by sublingual and/or buccal administration. Molded tablets, compressed tablets or freeze-dried tablets are exemplary forms which may be used.
  • compositions include those formulating the present compound(s) with fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins. Also included in such formulations may be high molecular weight excipients such as celluloses (avicel) or polyethylene glycols (PEG). Such formulations can also include an excipient to aid mucosal adhesion such as hydroxy propyl cellulose (HPC), hydroxy propyl methyl cellulose (HPMC), sodium carboxy methyl cellulose (SCMC), maleic anhydride copolymer (e.g., Gantrez), and agents to control release such as polyacrylic copolymer (e.g. Carbopol 934).
  • fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins.
  • high molecular weight excipients such as celluloses (avicel) or polyethylene glycols (P
  • Lubricants, glidants, flavors, coloring agents and stabilizers may also be added for ease of fabrication and use.
  • Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like.
  • the oral drug components can be combined with any oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like.
  • the pharmaceutical formulations according to the invention include those suitable for oral, parenteral (including subcutaneous, intradermal, intramuscular, intravenous [bolus or infusion], and intraarticular), inhalation (including fine particle dusts or mists which may be generated by means of various types of metered dose pressurized aerosols), nebulizers or insufflators, rectal, intraperitoneal and topical (including dermal, buccal, sublingual, and intraocular) administration, although the most suitable route may depend upon, for example, the condition and disorder of the recipient.
  • parenteral including subcutaneous, intradermal, intramuscular, intravenous [bolus or infusion], and intraarticular
  • inhalation including fine particle dusts or mists which may be generated by means of various types of metered dose pressurized aerosols
  • nebulizers or insufflators rectal, intraperitoneal and topical (including dermal, buccal, sublingual, and intraocular) administration, although the most suitable route may depend upon,
  • Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, pills or tablets each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid, for example as elixirs, tinctures, suspensions or syrups; or as an oil-in-water liquid emulsion or a water-in- oil liquid emulsion.
  • the active ingredient may also be presented as a bolus, electuary or paste.
  • a tablet may be made by compression or moulding, optionally with one or more accessory ingredients.
  • Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, lubricating, surface active or dispersing agent.
  • Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.
  • the tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein.
  • the present compounds can, for example, be administered in a form suitable for immediate release or extended release.
  • Immediate release or extended release can be achieved by the use of suitable pharmaceutical compositions comprising the present compounds, or, particularly in the case of extended release, by the use of devices such as subcutaneous implants or osmotic pumps.
  • the present compounds can also be administered liposomally.
  • Preferred unit dosage formulations are those containing an effective dose, as hereinbefore recited, or an appropriate fraction thereof, of the active ingredient.
  • formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents.
  • the formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing the active ingredient into association with the carrier which constitutes one or more accessory ingredients. In general the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.
  • Liposomes can be formed from a variety of phospholipids, 1,2-dipalmitoylphosphatidylcholine, phosphatidyl ethanolamine (cephaline), phosphatidylserine, phosphatidylinositol, diphosphatidylglycerol (cardiolipin) or phosphatidylcholine (lecithin).
  • Formulations for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents.
  • the formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze- dried (lyophilised) condition requiring only the addition of the sterile liquid carrier, for example saline or water-for-injection, immediately prior to use.
  • compositions for parenteral administration include injectable solutions or suspensions which can contain, for example, suitable non-toxic, parenterally acceptable diluents or solvents, such as polyethylene glycol, ethanol, 1,3-butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid, or Cremaphor.
  • suitable non-toxic, parenterally acceptable diluents or solvents such as polyethylene glycol, ethanol, 1,3-butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid, or Cremaphor.
  • compositions for nasal, aerosol or inhalation administration include solutions in saline, which can contain, for example, benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, and/or other solubilizing or dispersing agents such as those known in the art.
  • Formulations for rectal administration may be presented as a suppository with the usual carriers such as cocoa butter, synthetic glyceride esters or polyethylene glycol. Such carriers are typically solid at ordinary temperatures, but liquefy and/or dissolve in the rectal cavity to release the drug.
  • Formulations for topical administration in the mouth include lozenges comprising the active ingredient in a flavoured basis such as sucrose and acacia or tragacanth, and pastilles comprising the active ingredient in a basis such as gelatin and glycerine or sucrose and acacia.
  • Exemplary compositions for topical administration include a topical carrier such as Plastibase (mineral oil gelled with polyethylene).
  • the amount of active ingredient which is required to achieve a therapeutic effect will, of course, vary with the particular compound, the route of administration, the subject under treatment, including the type, species, age, weight, sex, and medical condition of the subject and the renal and hepatic function of the subject, and the particular disorder or disease being treated, as well as its severity.
  • An ordinarily skilled physician, veterinarian or clinician can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.
  • Oral dosages of the present invention when used for the indicated effects, will range between about 0.01 mg per kg of body weight per day (mg/kg/day) to about 100 mg/kg/day, preferably 0.01 mg per kg of body weight per day (mg/kg/day) to 10 mg/kg/day, and most preferably 0.1 to 5.0 mg/kg/day, for adult humans.
  • the compositions are preferably provided in the form of tablets or other forms of presentation provided in discrete units containing 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 5.0, 10.0, 15.0, 25.0, 50.0, 100, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated.
  • a medicament typically contains from about 0.01 mg to about 500 mg of the active ingredient, preferably from about 1 mg to about 100 mg of active ingredient.
  • the most preferred doses will range from about 0.1 to about 10 mg/kg/minute during a constant rate infusion.
  • compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily.
  • preferred compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using those forms of transdermal skin patches well known to those of ordinary skill in the art.
  • the dosage to be administered in the form of a transdermal delivery system, the dosage
  • administration will, of course, be continuous rather than intermittent throughout the dosage regimen.
  • the invention also provides the use of a compound of formula I, for the manufacture of a medicament for the treatment or prophylaxis of cancer.
  • the compounds and the pharmaceutical compositions of the invention may be used in the prophylaxis and treatment of diseases such as cancer, diseases caused by parasites, allergic diseases, Crohns disease, rheumatic diseases, tuberculosis, diabetes, Alzheimer's disease, inflammatory diseases, multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), Parkinson's disease and diseases caused by bacteria, viruses and fungus.
  • diseases such as cancer, diseases caused by parasites, allergic diseases, Crohns disease, rheumatic diseases, tuberculosis, diabetes, Alzheimer's disease, inflammatory diseases, multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), Parkinson's disease and diseases caused by bacteria, viruses and fungus.
  • the compounds of the invention find particular application in the treatment or prophylaxis of various cancer types, including but not limited to, cancer of the bone, breast, respiratory tract, brain, reproductive organs, bone marrow, digestive tract, urinary tract, eye, liver, skin, head, neck, thyroid, parathyroid, and metastatic forms thereof.
  • Proliferative disorders of the breast include, but are not limited to, invasive ductal carcinoma, invasive lobular carcinoma, ductal carcinoma, lobular carcinoma in situ, and metastatic breast cancer.
  • Proliferative disorders of the skin include, but are not limited to, basal cell carcinoma, squamous cell carcinoma, malignant melanoma, and Kaposi's sarcoma.
  • Proliferative disorders of the respiratory tract include, but are not limited to, lung cancer, doxorubicin resistant lung cancer, small cell and non- small cell lung carcinoma, bronchial adenoma, pleuropulmonary blastoma, and malignant mesothelioma.
  • Proliferative disorders of the brain include, but are not limited to, brain stem and hypothalamic glioma, cerebellar and cerebral astrocytoma, medulloblastoma, ependymal tumors, oligodendroglial tumors, meningiomas and neuroectodermal, and pineal tumors.
  • Proliferative disorders of the male reproductive organs include, but are not limited to, prostate, testicular and penis cancer.
  • Proliferative disorders of the female reproductive organs include, but are not limited to, uterine, cervical, ovarian, vaginal, vulval cancer, uterine sarcoma, ovarian germ cell tumor, ovarian cancer, doxorubicin resistant ovarian cancer, and cisplatin resistant ovarian cancer.
  • Proliferative disorders of the digestive tract include, but are not limited to, anal, colon, colorectal, esophageal, gall bladder, stomach, pancreatic, rectal, small intestine, and salivary gland cancer.
  • Proliferative disorders of the liver include, but are not limited to, hepatocellular carcinoma, cholangiocarcinoma, and primary liver cancer.
  • Proliferative disorders of the eye include, but are not limited to, intraocular melanoma, retinoblastoma, and rhabdomyosarcoma.
  • Proliferative disorders of the head include, but are not limited to, laryngeal, hypopharyngeal, nasopharyngeal, oropharyngeal, lip, oral, and metastatic paranasal sinus cancer.
  • Proliferative disorders of the lymphomas include, but are not limited to, T cell and B cell lymphomas, non-Hodgkins lymphoma, cutaneous T cell lymphoma, Hodgkins disease, vincristin resistant lymphoma, and lymphoma of the central nervous system.
  • Leukaemia includes, but is not limited to, acute myeloid leukaemia, chronic myelogenous leukaemia, acute lymphocytic leukaemia, chronic lymphocytic leukaemia, teponiside resistant leukaemia, and hairy cell leukaemia.
  • Proliferative disorders of the thyroid include, but are not limited to, thyroid cancer, thymoma, and malignant thymoma.
  • Proliferative disorders of bone marrow include, but are not limited to, myeloma, and doxorubicin resistant myeloma.
  • Proliferative disorders of the urinary tract include, but are not limited to, kidney cancer and bladder cancer.
  • Sarcomas include, but are not limited to, sarcoma of the soft tissue, osteosarcoma, malignant fibrous histiocytoma, lymphosarcoma, and rhabdomyosarcoma.
  • anticancer and antineoplastic compounds include, but are not limited to, alkylating agents, antimetabolites, anticancer camptothecin derivatives, plant-derived anticancer agents, antibiotics, enzymes, platinum coordination complexes, tyrosine kinase inhibitors, hormones and hormone antagonists, monoclonal antibodies, interferons, biological response modifiers and other anticancer agents.
  • alkylating agents include, but are not limited to, mechlorethamine, cyclophosphamide, ifosfamide, melphalan, chlorambucil, busulfan, mitobronitol, ranimustin, nimustin, temozolomide and carmustine;
  • antimetabolites include, but are not limited to, methotrexate, fluorouracil, cytarabine, gemcitabine, fludarabine, mercaptopurine, thioguanine, and azathioprine;
  • camptothecin derivatives include, but are not limited to, irinotecan, topotecan, and camptothecin;
  • plant-derived agents include, but are not limited to, vinca alkaloids e.g.
  • vinblastine and vincristine examples include, but are not limited to, actinomycin D, daunorubicin, and bleomycin.
  • enzyme effective as antineoplastic agent includes L-asparaginase.
  • coordination compounds include, but are not limited to, cisplatin and carboplatin;
  • examples of tyrosine kinase inhibitors include, but are not limited to, gefitinib, imatinib, sunitinib, nilotinib, dasatinib, erlotinib, and pazopanib;
  • examples of hormones and hormone related compounds include, but are not limited to, prednisone, dexamethasone, formestane, aminoglutethimide, anastrozole,
  • interferons include, but are not limited to, interferon a, interferon a-2a, interferon a- 2b, interferon ⁇ , interferon ⁇ -la, and interferon ⁇ - ⁇ ;
  • biological response modifiers include, but are not limited to, krestin, lentinan, sizofiran, picibanil, and ubenimex.
  • anticancer agents include, but are not limited to, mitoxantrone, procarbazine, dacarbazine, hydroxycarbamide, pentostatin, tretinoin, leuprorelin, flutamide, and aldesleukin.
  • Example 104 was obtained by a final desilylation step by the action of tetrabutylammonium fluoride.
  • A, D, E, R 1 to R 5 , and L are as defined in formula I.
  • the monomethylated compound IX'a (Intermediate 117) was obtained as a major product under these reaction conditions and used in the synthesis of Example 105. A minor component was obtained due to an additional methylation at the indole nitrogen. This dimethylated compound IX'b (Intermediate 118) was used in the synthesis of Example 106. Under the same reaction conditions, monomethylation at the indole nitrogen yielded IX"a (Intermediate 119) that was used in the synthesis of Example 107. As a minor product, the dimethylated compound IX"b (Intermediate 120) was obtained and used in the synthesis of Example 108.
  • Step 1 Serotonin hydrochloride (X) was dissolved in water (20 mg/mL). 3 Eq of potassium carbonate and 1 eq of di-tert-butyl dicarbonate were added and the mixture was stirred at room temperature for 24 h. The aqueous reaction mixture was extracted with EtOAc and the organic phase was washed with water, 1 M HC1 (aq) and brine. The solvent was removed in vacuo and the residue was purified by column chromatography on silica gel with Cf ⁇ CVMeOH as eluent to give tert-butyl 2-(5- hydroxy- 1 H-indo 1-3 -yl)ethylcarbamate (XI) .
  • Step 3 The alkylated derivative (XII) was dissolved in Ct ⁇ Cytrifluoroacetic acid 2: 1 (25 mg/mL) and kept at room temperature for 30-90 min. The reaction mixture was concentrated in vacuo, co-evaporated with toluene and the residue was purified by column chromatography on silica gel with EtOAc/MeOH/TEA as eluent to give the desired amine (XIII).
  • FMCA Fluorometric Microculture Cytotoxicity Assay
  • FMCA Fluorescein diacetate
  • 96-well plates were prepared as follows:
  • kinase inhibitors (dasatinib, pazopanib, sorafenib, and sunitinib) as described above.
  • the acute lymphoblastic leukaemia cell line CCRF-CEM (see e.g. Foley GE, et al. Cancer 1965, 18, 522-529) was used throughout.
  • medium was added to six empty wells (blank wells) and wells were filled with PBS and cell suspension and served as control wells. Si-values were then calculated for each compound-treated well as described above. All experiments were conducted twice and a new batch of plates was prepared for each experiment. The data obtained showed the activity of the example compounds compared to the comparative compounds.
  • 384-well plates were prepared as follows:
  • kinase inhibitors sorafenib, sunitinib, dasatinib, and pazopanib were diluted with PBS to a concentration which was ten-times higher than the desired starting concentration. Then, a Biomek 2000 liquid handling system was employed to serially dilute the compounds in a deep-well 384-well plate. From this plate, assay plates containing 5 ⁇ compound per well were prepared with the Biomek 2000. Certain compounds precipitated when diluted with PBS, and these compounds were therefore prepared in a 96-well plate manually as described above using culture medium RPMI 1640 instead of PBS.
  • the compounds were also tested at five concentrations, with five times serial dilution on the following cell types: CCRF-CEM, hTERT-RPEl (normal retinal epithelial cells), hRPTEpiC (normal renal cells) and PBMC (peripheral blood mononuclear cells). Each experiment was performed three times, except for PBMC and hRPTEpiC, which were performed twice. Sl-values were calculated, graphs were plotted using GraphPadPrism 5.0 (GraphPad Software Inc. La Jo 11a, CA) and ECso-values for each cell type and compound were determined from the curves.
  • the example compounds of the invention were active in the CCRF-CEM cell measurements, showing EC50 values less than 10 ⁇ .
  • Preferred compounds of the invention had EC50 values less than 1 ⁇ . More preferred compounds of the invention had EC50 values less than 0.1 ⁇ , and the reference compounds, sorafenib, sunitinib, dasatinib, and pazopanib, had EC50 values of 8.3 ⁇ , 14.1 ⁇ , 9.7 ⁇ , and 25.9 ⁇ respectively, in this assay.
  • Most of the compounds of the invention showed lower EC50 values than the reference compounds and data is presented in Table 1.
  • Example CCRF-CEM Example CCRF-CEM

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Abstract

The invention provides novel pyrimidine derivatives of formula I, to methods of preparing such compounds, to pharmaceutical compositions containing such compounds,and to methods for using such compounds in treatment of diseases including cancer; wherein R1, R2, R3, R4, R5, L, A, D, E, Z, and Y are as defined in the specification.

Description

NOVEL PYRIMIDINE DERIVATIVES
Field of Invention
This invention relates to novel pyrimidine derivatives of formula I, to methods of preparing such compounds, to pharmaceutical compositions containing such compounds, and to methods for using such compounds in treatment of diseases including cancer.
Background of Invention
Cancer is a major and often fatal disease. Accordingly, the development of new therapies for cancer is an ongoing process of outmost importance. The majority of cancers are present as solid tumours, such as lung cancer, breast cancer and prostate cancer, while others represent haemato logical and lymphoid malignancies, such as leukaemias and lymphomas.
During the recent decade much interest has been devoted to drugs directed to specific target molecules. Molecules regulating cell proliferation and death, such as Tyrosine Kinase Receptors (RTKs) for growth factors, are among targets for this type of therapeutic approach. Two classes of compounds targeting RTKs are currently used in clinical practice: monoclonal antibodies and tyrosine kinase inhibitors. The first approved targeted therapies were trastuzumab, a monoclonal antibody against HER2, for treatment of metastatic breast cancer, and imatinib, a small tyrosine kinase inhibitor targeting BCR-Abl, in Chronic Myeloid Leukemia. Despite good treatment results many of the treated patients have developed drug resistance, often due to the activation of alternative RTKs pathways. Currently there is a general idea that molecules interfering simultaneously with multiple RTKs might be more effective than single target agents. There are a few recently approved drugs, such as sorafenib and sunitinib, that apparently target multiple pathways and could serve as
representatives of this new generation of anti-cancer drugs (e.g. Gossage and Eisen, Targeting multiple kinase pathways: a change in paradigm. Clin Cancer Res (2010) vol. 16(7) pp. 1973-8).
Another example of an important target for cancer chemotherapy is tubulin. The targeting drugs in this therapy interrupt microtubule spindle-mediated chromosome segregation, arrest the dividing tumor cells in mitosis and subsequently induce apoptosis. Existing drugs are targeting microtubules via two main mechanisms, e.g. molecules of the taxane class (that stabilize the tubulins) and several vinca alkaloids (destabilizers). The potency, efficacy, and widespread clinical use of these agents of natural origin in a variety of cancers, e.g. breast, ovarian, prostate, lung, leukaemias, and lymphomas, stand testament to the importance of tubulin and its role in cancer growth. Derivatives and analogs of these plant compounds are constantly being isolated or synthesized to find more efficacious anticancer agents. For examples of novel tubulin polymerization inhibitors, see e.g. WO 2009/070645 and US
2010/0279410.
In the clinic cancer chemotherapy is used in attempts to cure or palliate the disease. In most cases this therapy is delivered in the form of combination chemotherapy, i.e. when two or more drugs having different modes of action are used together in order to optimise the effect on the cancer cells and to minimise side effects. The results obtained with chemotherapy vary according to tumour type. Some tumours are very sensitive and the treatment has a high probability of leading to beneficial treatment results including cure of the disease. Examples of this type of tumours are acute leukaemias, malignant lymphomas, testicular cancer, chorion carcinomas, and Wilms tumour. Other types of cancer chemotherapy can result in effective palliation and prolonged survival. Examples of such tumours are breast cancer, colorectal cancer, ovarian cancer, small-cell lung cancer, bladder cancer, multiple myeloma, and chronic leukaemias of both the lymphatic and myeloid type. Primary drug resistant tumours which respond poorly to classical chemotherapy include malignant glioma, melanoma, prostate cancer, sarcomas, and gastrointestinal tumours other than colorectal cancers (see e.g. DeVita, Hellman, and Rosenberg: Cancer: Principles & Practice of Oncology, Eighth Edition 978-0-7817-7207-5). Certain pyrimidine compounds and their potential use in the treatment of cancer are disclosed in for example WO2003/030909, WO2003/063794, WO2004/056807, WO2004/056786, US2004/220177, WO2005/013996, WO2006/133426,
WO2007/085833, WO2008/128231 and WO2009/063240. What is needed in the art are targeted drugs that work in a specific manner, being selective in eliminating subpopulations of cells involved in tumour survival and progression. The present invention provides novel pyrimidine compounds having a surprisingly efficient and selective antiproliferative activity. Hence, these novel compounds are useful in the treatment of proliferative diseases, such as cancer.
Description of the invention
The present invention provides compounds of formula I or a pharmaceutically acceptable ester, amide, solvate or salt thereof,
Figure imgf000004_0001
wherein
Z represents carbon or nitrogen;
Y represents carbon or nitrogen; wherein one of Z and Y represents nitrogen;
A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and D and E represents carbon; or A and D represent nitrogen and E represents carbon; or A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl, when D represents carbon;
R2 represents hydrogen or amino, when Y or Z represents carbon;
R3 represents hydrogen, (Ci-C3)alkyl, amino, trifluoromethyl or (Co-Ci)alkylaryl; R4 represents heteroaryl, optionally substituted with one or more substituents; and R5 represents hydrogen or methyl. In a first aspect of the invention, there is provided a compound of formula I or a pharmaceutically acceptable ester, amide, solvate or salt thereof,
Figure imgf000005_0001
wherein
Z represents carbon or nitrogen;
Y represents carbon or nitrogen; wherein one of Z and Y represents nitrogen;
A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and D and E represents carbon; or A and D represent nitrogen and E represents carbon; or A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl, when D represents carbon;
R2 represents hydrogen or amino, when Y or Z represents carbon;
R3 represents hydrogen, (Ci-C3)alkyl, amino, trifluoromethyl or (Co-Ci)alkylaryl; R4 represents heteroaryl, optionally substituted with one or more substituents; and R5 represents hydrogen or methyl;
provided that the compound N2,N4-bis(lH-indol-5-ylmethyl)pyrimidine-2,4-diamine is excluded. In another aspect of the invention, there is provided a compound of formula I, wherein Z, D and E represent carbon; and Y and A represent nitrogen. This is illustrated by formula la, wherein R1, R2, R3, R4, R5 and L are as defined under formula I above:
Figure imgf000006_0001
In another aspect of the invention, there is provided a compound of formula I, wherein Z and E represent carbon; and Y, D and A represent nitrogen. This is illustrated by formula lb, wherein R2, R3, R4, R5 and L are as defined under formula I above:
Figure imgf000006_0002
In another aspect of the invention, there is provided a compound of formula I, wherein Z and D represent carbon; and Y, E and A represent nitrogen. This is illustrated by formula Ic, wherein R1, R2, R3, R4, R5 and L are as defined under formula I above:
Figure imgf000007_0001
In another aspect of the invention, there is provided a compound of formula I, wherein Z, A and D represent carbon; and Y and E represent nitrogen. This is illustrated by formula Id, wherein R1, R2, R3, R4, R5 and L are as defined under formula I above:
Figure imgf000007_0002
In another aspect of the invention, there is provided a compound of formula I, wherein Y, D and E represent carbon; and Z and A represent nitrogen. This is illustrated by formula Ie, wherein R1, R2, R3, R4, R5 and L are as defined under formula I above:
Figure imgf000007_0003
In another aspect of the invention, there is provided a compound of formula I, wherein Y and E represent carbon; and Z, D and A represent nitrogen. This is illustrated by formula If, wherein R2, R3, R4, R5 and L are as defined under formula I above:
Figure imgf000008_0001
In another aspect of the invention, there is provided a compound of formula I, wherein Y and D represent carbon; and Z, E and A represent nitrogen. This is illustrated by formula Ig, wherein R1, R2, R3, R4, R5 and L are as defined under formula I above:
Figure imgf000008_0002
In another aspect of the invention, there is provided a compound of formula I, wherein Y, A and D represent carbon; and Z and E represent nitrogen. This is illustrated by formula Ih, wherein R1, R2, R3, R4, R5 and L are as defined under formula I above:
Figure imgf000009_0001
In another aspect of the invention, R4 represents heteroaryl that is optionally substituted with one or more substituents. Such substituents include, but are not limited to, halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (C1-C4)alkyl(C2- C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl(CO)0(Ci-C4)alkyl, (Ci- C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(C6-Cio)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci- C4)-alkyl], (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl(CN), (d-C4)alkyl(C6- Cio)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)(Ci- C4)alkyl, (C6-Cio)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6-Ci0)aryl-O(Ci- C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci- C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, 0(C6-
Cio)aryl, 0(CO)(Ci-C4)alkyl, O(CO)(C6-Ci0)aryl, OS02OH, NH(Ci-C4)alkyl, N[(Ci- C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(C6-Ci0)aryl, and CF3.
In another aspect of the invention, R4 represents heteroaryl that is optionally substituted with one or more substituents. Such substituents include, but are not limited to, halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (Ci-C4)alkyl(C2- C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl(CO)0(Ci-C4)alkyl, (Ci- C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl, (Ci-C4)alkyl(CO) H(Ci- C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl-0(Ci-C4)alkyl, (d-C4)alkyl-0(C6- Cio)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-0(CO)(C6-Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(CO)(C6-Ci0)aryl, (Ci- C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci- C4)alkyl(C6-Cio)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2- C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Cio)aryl, (C6-Cio)aryl-halogen, (C6-Cio)aryl-OH, (C6-Cio)aryl-NH2, (C6-Cio)aryl- 0(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci- C4)alkyl, (C2-C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2- C9)heterocyclyl(Ci-C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci- C4)alkyl, 0(Ci-C4)alkyl(C6-Cio)aryl, 0(Ci-C4)alkyl(C6-Cio)heteroaryl, 0(Ci- C4)alkyl(C2-C9)heterocyclyL 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, 0(C6-Cio)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2, O(CO)(C6-Ci0)aryl, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl],
NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl-NH2, NH(CO)(C6-Ci0)aryl, NHS02(Ci- C4)alkyl, S02NH2, and CF3.
In another aspect of the invention, R4 represents heteroaryl that is optionally substituted with one or more substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci- C4)alkyl(CO)NH(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl-OH, (Ci-C4)alkyl-0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl- 0(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-0(CO)(C6- Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci- C4)alkyl] [(C i -C4)-alkyl] , (C i -C4)alkyl-NH(CO)(C i -C4)alkyl, (C i -C4)alkyl- NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(CO)(C6-Ci0)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Cio)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci- C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6- Cio)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Cio)aryl-halogen, (C6-Cio)aryl-OH, (C6-Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci- C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci- C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci- C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, 0(Ci- C4)alkyl(C6-Cio)aryl, 0(Ci-C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2, O(CO)(C6-Ci0)aryl, OCF3, OS02(Ci- C4)alkyl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci- C4)alkyl, NH(CO)(Ci-C4)alkyl-NH2, NH(CO)(C6-Ci0)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3. In another aspect of the invention, R4 represents heteroaryl that is optionally substituted with one or more substituents selected from hydroxy, methyl, and methoxy.
In another aspect of the invention, L represents a bond.
In another aspect of the invention, L represents Ci-alkyl (methylene).
In another aspect of the invention, L represents C2-alkyl (ethylene).
In another aspect of the invention, there is provided a compound of formula I, wherein L-R4 is selected from:
Figure imgf000011_0001
Figure imgf000012_0001
wherein R6 is selected from hydrogen and (Ci-C4)alkyl;
R7 is selected from hydrogen, halogen, nitro, cyano, (Ci-C4)alkyl, (Ci- C4)alkyl(CO)OH, (Ci-C )alkyl(CO)0(Ci-C4)alkyl, (Ci-C )alkyl(CO)NH2, (d- C4)alkyl(CO)NH(Ci-C4)alkyl, (C]-C4)alkyl-OH, (Ci-C4)alkyl-O(C6-Ci0)aryl, (d- C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (Ci- C4)alkyl(C2-C9)heterocyclyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci- C4)alkyl, (CO)(Ci-C4)alkyl, (C6-C]0)aryl, (C6-Ci0)aryl-halogen, (C6-C10)aryl-OH, (C6- Cio)aryl-0(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-
C9)heteroaryl-OH, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, and (C2-C9)heterocyclyl(Ci-C4)alkyl-OH;
R8 is selected from hydrogen, halogen, nitro, cyano, hydroxy, amino, (Ci-C4)alkyl, (C i -C4)alky l(C2-C9)heterocyclyl, (C i -C4)alkyl-NH2, (C i -C4)alky 1-NH(C i -C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-NH(CO)(C6-Cio)aryl, (CO)(Ci-C4)alkyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(Ci-C9)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(C6- Cio)aryl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci- C4)alkyl, NH(CO)(C6-Cio)aryl, CF3, (C6-Cio)aryl, (C6-Ci0)aryl-halogen, (C6-Cio)aryl- OH, (C6-Cio)aryl-0(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci- C9)heteroaryl-OH, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, and (C2-C9)heterocyclyl(Ci-C4)alkyl-OH;
R9 is selected from hydrogen, halogen, (Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, and
Figure imgf000013_0001
R10 is selected from hydrogen, halogen, hydroxy, (Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci- C4)alkyl-0(Ci-C4)alkyl, and 0(Ci-C4)alkyl; and
R1 1 is selected from hydrogen, hydroxy, (Ci-C4)alkyl, and 0(Ci-C4)alkyl.
In another aspect of the invention, there is provided a compound of formula I, wherein L-R4 is selected from:
Figure imgf000013_0002
Figure imgf000014_0001
wherein R6 is selected from hydrogen and (Ci-C4)alkyl;
R7 is selected from hydrogen, halogen, nitro, cyano, (Ci-C4)alkyl, (Ci- C4)alkyl(CO)OH, (Ci-C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (d- C4)alkyl(CO)NH(Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci-C4)alkyl-0(C6-Cio)aryl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-0(CO)(C6-Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Cio)aryl, (Ci- C4)alkyl(C2-C9)heterocyclyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci- C4)alkyl, (CO)(Ci-C4)alkyl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-0(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci- C9)heteroaryl-OH, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, and (C2-C9)heterocyclyl(Ci-C4)alkyl-OH;
R8 is selected from hydrogen, halogen, nitro, cyano, hydroxy, amino, (Ci-C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-NH(CO)(C6-Cio)aryl, (CO)(Ci-C4)alkyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, 0(Ci-C4)alkyl, 0(Ci-C4)alkyl(C6-Cio)aryl, 0(Ci- C4)alkyl(Ci-C9)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(C6- Cio)aryl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci- C4)alkyl, NH(CO)(C6-Cio)aryl, CF3, (C6-Cio)aryl, (C6-Cio)aryl-halogen, (C6-Cio)aryl- OH, (C6-Cio)aryl-0(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci- C9)heteroaryl-OH, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, and (C2-C9)heterocyclyl(Ci-C4)alkyl-OH;
R9 is selected from hydrogen, halogen, (Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, and (C6-C10)aryl;
R10 is selected from hydrogen, halogen, hydroxy, (Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci- C4)alkyl-0(Ci-C4)alkyl, and 0(Ci-C4)alkyl; and
R1 1 is selected from hydrogen, hydroxy, (Ci-C4)alkyl, and 0(Ci-C4)alkyl.
In another aspect of the invention, there is provided a compound of formula I, wherein L-R4 is selected from:
Figure imgf000015_0001
wherein R6 represents hydrogen;
R7 is selected from hydrogen and (Ci-C4)alkyl, preferably methyl;
R8, R9 and R10 represents hydrogen; and
R11 is selected from hydrogen, hydroxy, (Ci-C4)alkyl, preferably methyl, and 0(Ci- C4)alkyl, preferably methoxy.
In another aspect of the invention, there is provided a compound of formula I, wherein L-R4 is selected from:
Figure imgf000016_0001
wherein R6 is selected from hydrogen and (Ci-C4)alkyl;
R7 is selected from hydrogen, halogen, nitro, cyano, (Ci-C4)alkyl, (Ci- C4)alkyl(CO)OH, (Ci-C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (d- C4)alkyl(CO)NH(Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci-C4)alkyl-0(C6-Cio)aryl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-0(CO)(C6-Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Cio)aryl, (Ci- C4)alkyl(C2-C9)heterocyclyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci- C4)alkyl, (CO)(Ci-C4)alkyl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-0(Ci-C4)alkyl, (Ci-Cc))heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci- C9)heteroaryl-OH, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, and (C2-C9)heterocyclyl(Ci-C4)alkyl-OH;
R7a and R7b are independently selected from hydrogen and (Ci-C4)alkyl, preferably methyl;
R8 is selected from hydrogen, halogen, nitro, cyano, hydroxy, amino, (Ci-C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-NH(CO)(C6-Cio)aryl, (CO)(Ci-C4)alkyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(Ci-C9)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, OCF3, 0(CO)(Ci-C4)alkyl, 0(CO)(C6-Cio)aryl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(C6-Ci0)aryl, CF3, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Cio)aryl-OH, (C6-Cio)aryl-0(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2- C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, and (C2-C9)heterocyclyl(Ci- C4)alkyl-OH;
R9 is selected from hydrogen, halogen, (Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, and
Figure imgf000017_0001
R1 1 is selected from hydrogen, hydroxy, (Ci-C4)alkyl, and 0(Ci-C4)alkyl.
In another aspect of the invention, there is provided a compound of formula I, wherein L-R4 is
Figure imgf000017_0002
wherein R6 and R8 represent hydrogen;
R7a and R7b are independently selected from hydrogen and (Ci-C4)alkyl, preferably methyl. In another aspect of the invention, there is provided a compound of formula I, wherein R5 represents hydrogen.
In another aspect of the invention, there is provided a compound of formula I, wherein R5 represents methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein R2 represents amino.
In another aspect of the invention, there is provided a compound of formula I, wherein R2 represents hydrogen.
In another aspect of the invention, there is provided a compound of formula I, wherein R2 represents hydrogen; and R3 represents hydrogen, methyl, trifluoromethyl or benzyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Z, D and E represent carbon;
Y and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO) H(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (Ci-C4)alkyl-0(C6-Cio)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl- NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH,
(CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci- C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, (C2- C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyL 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci- C4)alkyl-NH2, O(CO)(C6-Ci0)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci- C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Z and E represent carbon;
Y, D, and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R2 represents hydrogen; R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-
C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO) H(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl- NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH,
(CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci- C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, (C2-
C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyL 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci- C4)alkyl-NH2, O(CO)(C6-Ci0)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-
C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl. In another aspect of the invention, there is provided a compound of formula I, wherein Z and D represent carbon;
Y, E and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-
C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl- NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH,
(CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci- C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, (C2-
C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyL 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci- C4)alkyl-NH2, O(CO)(C6-Ci0)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci- C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl. In another aspect of the invention, there is provided a compound of formula I, wherein Z, A and D represent carbon;
Y and E represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-
C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO) H(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl- NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH,
(CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci- C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, (C2-
C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyL, 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci- C4)alkyl-NH2, 0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci- C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Y, D and E represent carbon;
Z and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-
C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO) H(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl- NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH,
(CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9) eteroaryl-OH, (Ci-C9) eteroaryl-NH2, (Ci-C9)heteroaryl(Ci- C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, (C2- C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyL 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci- C4)alkyl-NH2, 0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci- C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Y and E represent carbon;
Z, D, and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO) H(Ci-
C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl- NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci- C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, (C2- C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyL, 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci- C4)alkyl-NH2, O(CO)(C6-Ci0)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci- C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Y and D represent carbon;
Z, E and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci-
C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (Ci-C4)alkyl-0(C6-Cio)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl- NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH,
(CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci- C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, (C2- C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyL 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci- C4)alkyl-NH2, O(CO)(C6-Ci0)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci- C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Y, A and D represent carbon;
Z and E represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen;
R2 represents hydrogen;
R3 represents hydrogen or methyl; R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci-
C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl- 0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci- C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl- NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH,
(CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6-Ci0)aryl-halogen, (C6-Ci0)aryl-OH, (C6- Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci- C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2- C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-C4)alkyl-OH, (C2- C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, O(Ci-C4)alkyl(C6-Ci0)aryl, 0(Ci- C4)alkyl(C6-Cio)heteroaryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl(Ci-C4)alkyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-C4)alkyl, O(C6-Ci0)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci- C4)alkyl-NH2, O(CO)(C6-Ci0)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci- C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Z, D and E represent carbon; Y and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Z and E represent carbon;
Y, D, and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Z and D represent carbon;
Y, E and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy; and
R5 represents hydrogen or methyl. In another aspect of the invention, there is provided a compound of formula I, wherein Z, A and D represent carbon;
Y and E represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Y, D and E represent carbon;
Z and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy; and
R5 represents hydrogen or methyl. In another aspect of the invention, there is provided a compound of formula I, wherein Y and E represent carbon;
Z, D, and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Y and D represent carbon;
Z, E and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Y, A and D represent carbon;
Z and E represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen;
R2 represents hydrogen;
R3 represents hydrogen or methyl; R4 represents a heteroaryl selected from indolyl, indazolyl, and benzimidazolyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of hydroxy, methyl, and methoxy; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Z, D and E represent carbon;
Y and A represent nitrogen;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
L-R4 is selected from:
Figure imgf000031_0001
R7 represents hydrogen or methyl;
R6 represents hydrogen or methyl;
R8 is selected from hydrogen, methyl, fluoro, methoxy, ethoxy and OCF3
R11 is selected from hydrogen, methyl, and methoxy; and
R5 represents hydrogen or methyl.
In another aspect of the invention, there is provided a compound of formula I, wherein
Y, D and E represent carbon;
Z and A represent nitrogen;
R1, R2, and R3 represent hydrogen;
L-R4 is selected from:
Figure imgf000032_0001
R6 represents hydrogen or methyl;
R8 represents hydrogen;
R7 is selected from hydrogen, methyl, (Ci-C4)alkyl-OH, and COOCH3;
R7a and R7b is independently selected from hydrogen and methyl; and
R5 represents hydrogen or methyl. In another aspect of the invention, there is provided a compound of formula I, wherein
Z represents carbon or nitrogen;
Y represents carbon or nitrogen, wherein one of Z and Y represents nitrogen;
A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and D and E represents carbon; or A and D represent nitrogen and E represents carbon; or A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
R1 represents hydrogen or methyl, when D represents carbon;
R2 represents hydrogen or amino;
R3 represents hydrogen, methyl, trifluoromethyl or (Co-Ci)alkylaryl;
R5 represents hydrogen or methyl;
L-R4 is selected from:
Figure imgf000032_0002
Figure imgf000033_0001
R6 is selected from hydrogen and methyl;
R7 is selected from hydrogen, methyl, (Ci-C4)alkyl-OH and (CO)OCH3;
R7a and R7b are independently selected from hydrogen, and methyl;
R8 is selected from halogen, hydrogen, hydroxy, (CO)OH, (CO)OCH3, 0(Ci-C4)alkyl, 0(Ci-C4)alkyl(C6-Cio)aryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl, 0(EtO)i_3(Ci-C4)alkyl, and OCF3;
R9 and R10 represent hydrogen; and
R11 is selected from hydrogen, methyl, and 0(Ci-C4)alkyl.
In another aspect of the invention, there is provided a compound of formula I, wherein Z represents carbon or nitrogen;
Y represents carbon or nitrogen, wherein one of Z and Y represents nitrogen;
A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and
D and E represents carbon; or A and D represent nitrogen and E represents carbon; or
A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
R1 represents hydrogen or methyl, when D represents carbon;
R2 represents hydrogen or amino; R3 represents hydrogen, methyl, trifluoromethyl or (Co-Ci)alkylaryl;
R5 represents hydrogen or methyl;
L-R4 is selected from:
Figure imgf000034_0001
R6 is selected from hydrogen and methyl;
R7 is selected from hydrogen, methyl, (Ci-C4)alkyl-OH, and (CO)OCH3,
R7a and R7b are independently selected from hydrogen, and methyl;
R8 is selected from halogen, hydrogen, hydroxy, (CO)OH, (CO)OCH3, 0(Ci-C4)alkyl, 0(Ci-C4)alkyl(C6-Cio)aryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl, 0(EtO)i_3(Ci-C4)alkyl, and OCF3;and
R1 1 is selected from hydrogen, methyl, and 0(Ci-C4)alkyl.
In another aspect of the invention, there is provided a compound of formula I, wherein L represents a bond or (C2)alkyl.
In another aspect of the invention, there is provided a compound of formula I, said compound being selected from:
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4-diamine; N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamm^ N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-5-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-ylmethyl)pyrimidm^ diamine;
N2-(lH-indazol-5-ylmethyl)-N4-(lH-indol-5-ylmethyl)pyrimidine-2,4-diamine;
N2-(lH-benzo[JJimidazol-5-ylmethyl)- -(lH-indol-5-ylmethyl)pyrimidine-2,^ diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-6-ylmethyl)pyrimidine-2,4-diamine; N2 2-(lH-indol-3-yl)ethyl]-N4-(lH-indol-5-ylmethyl)pyrimidine-2,4-diamine; 3 - {2- [4-( lH-indo 1-5 -ylmethylamino)-pyrimidin-2-ylamino] ethyl} - lH-indo 1-5 -ol; A^-(lH-indol-5-ylmethyl)-N2 2-(5-methyl-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
N2-(lH-indol-4-yl)-N4-(2-methyl-lH-indol-5ylmethyl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-ylmethyl)pyrimidine-2,4- diamine;
N2,N4-Bis-(2-methyl-lH-indol-5-ylmethyl)pyrimidine-2,4-diamine;
N2-(lH-indazol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-ylmethyl)pyrimidine-2,4- diamine;
N2-(2-(lH-indol-3-yl)-ethyl)-A^-(2-methyl-lH-indol-5ylmethyl)pyrimidine-2,4- diamine;
N2-(lH-indol-4-yl)-N4-(lH-indazol-5ylmethyl)pyrimidine-2,4-diamine;
N4-(lH-benzo[JJimidazol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-6-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-6-ylmethyl)pyrimidine-2,4-diamine;
N2,N4-bis-(lH-indol-6-ylmethyl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamine;
N4-[2-(lH-indol-3-yl)ethyl]-N2-(lH-indol-5-ylmethyl)pyrimidine-2,4-diamine; 3 - {2- [2-( lH-indo 1-5 -ylmethylamino)-pyrimidin-4-ylamino] ethyl} - lH-indo 1-5 -ol; N2-( lH-indol-5 -ylmethyl)-^4- [2-(5 -methyl- lH-indol-3 -yl)ethyl]pyrimidine-2,4- diamine;
N2-(lH-indol-5-ylmethyl)-A^-[2-(5-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
N2-(lH-indazol-5-ylmethyl)-N4-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-4-yl)-N4-(lH-indol-5ylmethyl)-6-methylpyrimidine-2,4-diamine;
N2,N4-bis(lH-indol-5-ylmethyl)-6-methylpyrimidine-2,4-diamine;
3 - {2- [4-( lH-indo 1-5 -ylmethylamino)-6-methyl-pyrimidin-2-ylamino] -ethyl} - 1H- indol-5-ol;
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-yl)-6-trifluoromethylpyrimidine- 2,4-diamine;
N2,N4-bis-(lH-indol-5-ylmethyl)-6-trifluoromethylpyrimidine-2,4-diamine;
N2-(2-(lH-indol-3-yl)ethyl)-N4-(lH-indol-5-ylmethyl)-6-trifluoromethylpyrimidine- 2,4-diamine;
N2,N4-bis(lH-indol-5-ylmethyl)-6-benzylpyrimidine-2,4-diamine;
A^-(lH-indazol-5-ylmethyl)-N2-(lH-indol-4-yl)-6-methylpyrimidine-2,4-diamine;
-(lH ndol-5-ylmethyl)-N2-(lH ndol-4-yl)pyrimidine-2,4,5-triamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)pyrimidine-2,4,5-triamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-6-yl)pyrimidine-2,4,5-triamine; and
N2,N4-bis(lH-indol-5-ylmethyl)pyrimidine-2,4,5-triamine.
In another aspect of the invention, there is provided a compound of formula I, said compound being selected from:
5-{[2-(lH-indol-5-ylmethylamino)pyrimidin-4-ylamino]methyl}indolin-2-one; N4-(2-methyl-lH-indol-5-yl)-N2-(2-methyl-lH-indol-5-ylmethyl)pyrimidine-2,4- diamine;
N2-[2-(5-methoxy- lH-indol-3-yl)ethyl]-N4-(2-methyl- lH-indol-5- ylmethyl)pyrimidine-2,4-diamine;
N2-(lH-indazol-5-ylmethyl)-N4-(2 -methyl- lH-indol-5-yl)pyrimidine-2,4-diamine; N4-( lH-indazol-5 -ylmethyl)-N2- [2-(5 -methoxy- lH-indol-3 -yl)ethyl]pyrimidine-2,4- diamine;
N2-(lH-benzo[ ]imidazol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrim
diamine;
N4-(lH-benzo[ ]imidazol-5-ylmethyl)-N2-(lH-indol-5-ylmethyl)pyrim
diamine;
N*-( lH-benzo [ ]imidazol-5 -ylmethyl)-N2- [2-(5 -methoxy- lH-indol-3 - yl)ethyl]pyrimidine-2,4-diamine;
N2-(lH-indol-6-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamine; A^-(lH-indol-6-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
A^-(lH-indol-5-ylmethyl)-N2-{2 5-(benzyloxy)-lH-indol-3-yl]ethyl}pyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-{2-[5-(2-morpholinoethoxy)-lH-indol-3- yl]ethyl}pyrimidine-2,4-diamine;
N*-( lH-indol-5 -ylmethyl)-N2- {2- [5 -(2-methoxyethoxy)- lH-indol-3 - yl]ethyl}pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(l -methyl- lH-indol-4-yl)pyrimidine-2,4-diamine; N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-4-yl)pyrimidine-2,4-diamine;
A^-(lH-indol-5-ylmethyl)-N2-[(l-methyl-lH-indol-5-yl)methyl]pyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-ylmethyl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[(9H-carbazol-3-yl)methyl]pyrimidine-2,4-diamine; N2-(lH-indol-5-ylmethyl)-N4-[(9H-carbazol-3-yl)methyl]pyrimidine-2,4-diamine; Methyl 4-[4-(lH-indol-5-ylmethylamino)pyrimidin-2-ylamino]-lH-indole-6- carboxylate;
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-5-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-6-methyl-N4-(2 -methyl- lH-indol-5-yl)pyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]-6- methylpyrimidine-2,4-diamine; and Λ^-( lH-indol-5 -ylmethyl)-6-benzyl-N2- [2-(5 -methoxy- lH-indol-3 - yl)ethyl]pyrimidine-2,4-diamine.
In another aspect of the invention, there is provided a compound of formula I, said compound being selected from:
N*-( lH-indol-5 -ylmethyl)-N2- [2-(5 -methoxy-7-methyl- 1 H-indol-3 - yl)ethyl]pyrimidine-2,4-diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(5-ethoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
Λ^-( lH-indol-5 -ylmethyl)-N2- {2- [5 -(trifiuoromethoxy)- lH-indol-3 - yl]ethyl}pyrimidine-2,4-diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(5-fluoro-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(6-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(7-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
N2-(lH-indol-5-ylmethyl)-N4-(l,2-dimethyl-lH-indol-5-yl)pyrimidine-2,4-diamine; methyl 5-[2-(lH-indol-5-ylmethylamino)pyrimidin-4-ylamino]-lH-indole-2- carboxylate;
N2-(lH-indol-5-ylmethyl)-N4-(2,3-dimethyl-lH-indol-5-yl)pyrimidine-2,4-diamine; N2-(lH-indol-5-ylmethyl)-N4-(lH-benzo[ ]imidazol-5-yl)pyrimidine-2,4-diamine; N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-benzo[(i]imidazol-5-yl)pyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-4-yl)-6-methylpyrimidine-2,4-diamine; N2- [2-(5-methoxy- lH-indol-3 -yl)ethyl] -6-methyl-N4- [(2-methyl- lH-indol-5 - yl)methyl]pyrimidine-2,4-diamine;
N*-( lH-indazol-5 -ylmethyl)-N2- [2-(5 -methoxy- lH-indol-3 -yl)ethyl] -6- methylpyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-2-methyl-lH-indol-3-yl)ethyl]-6- methylpyrimidine-2,4-diamine; A^-(lH-indol-5-ylmethyl)-N2 2-(4-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2 diamine;
4- [4-( lH-indo 1-5 -ylmethylamino)pyrimidin-2-ylamino] - lH-indo le-6-carboxylic acid; N2-( lH-indol-4-yl)-6-methyl-N^
diamine;
{5 - [2-( lH-indo 1-5 -ylmethylamino)pyrimidin-4-ylamino] - lH-indo 1-2-yl} methano 1; N2-(lH-indol-5-ylmethyl)-A^-methyl-A^-(2-methyl-lH-indol-5-yl)pyrimidine-2,4- diamine;
N2-(lH-indol-5-ylmethyl)-A^-(l,2-dimethyl-lH-indol-5-yl)-A^-methylpyrimidine-2,4 diamine;
N*-( lH-indol-5 -ylmethyl)-N2- [2-(5 -methoxy- 1 -methyl- 1 H-indol-3 - yl)ethyl]pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-l-methyl-lH-indol-3-yl)ethyl]-N2- methylpyrimidine-2,4-diamine; and
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]-N2- methylpyrimidine-2,4-diamine.
In preferred aspect of the invention, there is provided a compound of formula I, said compound being selected from:
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-5-yl)pyrimidine-2,4-diamine;
N2-[2-(lH-indol-3-yl)ethyl]-N4-(lH-indol-5-ylmethyl)pyrimidine-2,4-diamine;
3-{2-[4-(lH-indol-5-ylmethylamino)-pyrimidin-2-ylamino]ethyl}-lH-indol-5-ol;
A^-(lH-indol-5-ylmethyl)-N2-[2-(5-methyl-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
N2-( lH-indol-4-yl)-N4-(2 -methyl- lH-indol-5ylmethyl)pyrimidine-2,4-diamine; N2-(2-(lH-indol-3-yl)-ethyl)-A^-(2-methyl H-indol-5ylmethyl)pyrimidine-2,4- diamine;
N2-(lH-indol-4-yl)-N4-(lH-indazol-5ylmethyl)pyrimidine-2,4-diamine;
N4-(lH-benzo[ ]imidazol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4-di N4-(lH-indol-6-ylmethyl)-N -(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamm N4-[2-(lH-indol-3-yl)ethyl]-N2-(lH-indol-5-ylmethyl)pyrimidine-2,4-diamm^ 3 - {2- [2-( lH-indo 1-5 -ylmethylamino)-pyrimidin-4-ylamino] ethyl} - lH-indo 1-5 -ol; N2-(lH-indol-5-ylmethyl)-A^-[2-(5-methyl-lH-indol-3-yl)ethyl]pyrimidine diamine;
N2-(lH-indol-5-ylmethyl)-A^-[2-(5-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
N2-(lH-indazol-5-ylmethyl)-N4-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-( lH-indol-4-yl)-N4-( lH-indol-5ylmethyl)-6-methylpyrimidine-2,4-diamine; 3 - {2- [4-( lH-indo 1-5 -ylmethylamino)-6-methyl-pyrimidin-2-ylamino] -ethyl} - 1H- indol-5-ol;
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-yl)-6-trifluoromethylpyrimidine 2,4-diamine;
N2-(2-(lH-indol-3-yl)ethyl)-A^-(lH-indol-5-ylmethyl)-6-trifluoromethylpyrimidine- 2,4-diamine;
N4-(lH-indazol-5-ylmethyl)-N2-(lH-indol-4-yl)-6-methylpyrimidine-2,4-diamine; N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4,5-triamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)pyrimidine-2,4,5-triamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-6-yl)pyrimidine-2,4,5-triamine;
A^-(2-methyl-lH-indol-5-yl)-N2-(2-methyl-lH-indol-5-ylmethyl)pyrimidine-2,4- diamine;
N2-[2-(5-methoxy- lH-indol-3-yl)ethyl]-N4-(2-methyl- lH-indol-5- ylmethyl)pyrimidine-2,4-diamine;
N2-(lH-indazol-5-ylmethyl)-N4-(2 -methyl- lH-indol-5-yl)pyrimidine-2,4-diamine; N4-( lH-indazol-5 -ylmethyl)-N2- [2-(5 -methoxy- lH-indol-3 -yl)ethyl]pyrimidine-2,4- diamine;
N2-(lH-benzo[ ]imidazol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrim
diamine;
A^-(lH-benzo[ ]imidazol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3- yl)ethyl]pyrimidine-2,4-diamine;
N2-(lH-indol-6-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamine
A^-(lH-indol-6-ylmethyl)-N2 2-(5-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
A^-(lH-indol-5-ylmethyl)-N2-{2 5-(benzyloxy)-lH-indol-3-yl]ethyl}pyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-{2-[5-(2-morpholinoethoxy)-lH-indol-3- yl]ethyl}pyrimidine-2,4-diamine;
N*-( lH-indol-5 -ylmethyl)-N2- {2- [5 -(2-methoxyethoxy)- lH-indol-3 - yl]ethyl}pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(l -methyl- lH-indol-4-yl)pyrimidine-2,4-diamine; N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-4-yl)pyrimidine-2,4-diamine;
Methyl 4- [4-( lH-indo 1-5 -ylmethylamino)pyrimidin-2-ylamino] - lH-indo le-6- carboxylate;
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-5-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-6-methyl-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]-6- methylpyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-6-benzyl-N2-[2-(5-methoxy-lH-indol-3- yl)ethyl]pyrimidine-2,4-diamine;
N*-( lH-indol-5 -ylmethyl)-N2- [2-(5 -methoxy-7-methyl- 1 H-indol-3 - yl)ethyl]pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-ethoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine; Λ^-( lH-indol-5 -ylmethyl)-N2- {2- [5 -(trifluoromethoxy)- lH-indol-3 - yl]ethyl}pyrimidine-2,4-diamine;
A^-(lH-indol-5-ylmethyl)-N2 2-(5-fluoro-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
A^-(lH-indol-5-ylmethyl)-N2 2-(6-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2 diamine;
A^-(lH-indol-5-ylmethyl)-N2 2-(7-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
N2-(lH-indol-5-ylmethyl)-N4-(l,2-dimethyl-lH-indol-5-yl)pyrimidine-2,4-di methyl 5-[2-(lH-indol-5-ylmethylamino)pyrimidin-4-ylamino]-lH-indole-2- carboxylate;
N2-(lH-indol-5-ylmethyl)-N4-(2,3-dimethyl-lH-indol-5-yl)pyrimidine-2,4-diamine; N2-(lH-indol-5-ylmethyl)-N4-(lH-benzo[ ]imidazol-5-yl)pyrimidine-2,4-diamine; N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-benzo[ ]imidazol-5-yl)pyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-4-yl)-6-methylpyrimidine-2,4-diamine; N2- [2-(5-methoxy- lH-indol-3 -yl)ethyl] -6-methyl-N4- [(2-methyl- lH-indol-5 - yl)methyl]pyrimidine-2,4-diamine;
N*-( lH-indazol-5 -ylmethyl)-N2- [2-(5 -methoxy- lH-indol-3 -yl)ethyl] -6- methylpyrimidine-2,4-diamine;
N*-( lH-indol-5 -ylmethyl)-N2- [2-(5 -methoxy-2-methyl- 1 H-indol-3 -yl)ethyl] -6- methylpyrimidine-2,4-diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(4-methoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
4-[4-(lH-indol-5-ylmethylamino)pyrimidin-2-ylamino]-lH-indole-6-carboxylic acid; N2-(lH-indol-4-yl)-6-methyl-N4-[(2-methyl-lH-indol-5-yl)methyl]pyrimidine-2,4- diamine;
{5 - [2-( lH-indo 1-5 -ylmethylamino)pyrimidin-4-ylamino] - lH-indo 1-2-yl} methano 1; N2-(lH-indol-5-ylmethyl)-A^-methyl-A^-(2-methyl-lH-indol-5-yl)pyrimidine-2,4- diamine; ^-(lH-indol-S-ylmethylVN4-^
diamine;
N*-( lH-indol-5 -ylmethyl)-N2- [2-(5 -methoxy- 1 -methyl- 1 H-indol-3 - yl)ethyl]pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-l-methyl-lH-indol-3-yl)ethyl]-N2- methylpyrimidine-2,4-diamine; and
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]-N2- methylpyrimidine-2,4-diamine. In another aspect of the invention, there is provided a compound of formula I, for use in therapy.
In another aspect of the invention, there is provided a compound of formula I, for use in treatment of cancer.
In another aspect of the invention, there is provided use of a compound of formula I, in the manufacture of a medicament and pharmaceutical compositions for treatment of cancer. In another aspect of the invention, there is provided a pharmaceutical composition comprising a compound of formula I, together with pharmaceutically acceptable diluents and carriers.
In another aspect of the invention, there is provided a method for treatment of cancer, which comprises administering to a subject in need thereof, a therapeutically effective amount of a compound of formula I.
In another aspect of the invention, there is provided a method for treatment of cancer, which comprises administering to a subject in need thereof, a therapeutically effective amount of a compound of formula I, in combination with another compound of formula I, in combination with radiation therapy, or in combination with another anticancer agent selected from alkylating agents, antimetabolites, anticancer camptothecin derivatives, plan-derived anticancer agents, antibiotics, enzymes, platinum coordination complexes, tyrosine kinase inhibitors, hormones, hormone antagonists, monoclonal antibodies, interferons, and biological response modifiers.
In all lists and in the Examples, the compound names were generated in accordance with IUPAC by ChemBioDraw Ultra version 11.0.
In another aspect of the invention, there is provided a number of intermediate compounds, comprising the following:
N-(lH-indol-5-ylmethyl)-2-chloro-pyrimidin-4-amine;
N-(lH-indol-5-ylmethyl)-4-chloro-pyrimidin-2-amine;
N-(2 -methyl- lH-indol-5-ylmethyl)-2-chloro-pyrimidin-4-amine;
N-(lH-indazol-5-ylmethyl)-2-chloro-pyrimidin-4-amine;
N-(lH-benzo[(i]imidazol-5-ylmethyl)-2-chloro-pyrimidin-4-amine;
N-(lH-indol-6-ylmethyl)-2-chloro-pyrimidin-4-amine;
N-(lH-indol-6-ylmethyl)-4-chloro-pyrimidin-2-amine;
N-(lH-indol-5-ylmethyl)-2-chloro-6-methyl-pyrimidin-4-amine;
N-(lH-indol-5-ylmethyl)-4-chloro-6-methyl-pyrimidin-2-amine;
N-(lH-indazol-5-ylmethyl)-2-chloro-6-methyl-pyrimidin-4-amine;
N-(lH-indol-5-ylmethyl)-2-chloro-6-trifluoromethyl-pyrimidin-4-amine;
N-(lH-indol-5-ylmethyl)-6-benzyl-2-chloro-pyrimidin-4-amine;
N-(lH-indol-5-ylmethyl)-2-chloro-5-nitro-pyrimidin-4-amine;
N-(lH-indol-4-yl)-2-chloro-pyrimidin-4-amine;
N-[2-(lH-indol-3-yl)ethyl]-2-chloro-pyrimidin-4-amine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)-5-nitropyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)-5-nitropyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-6-yl)-5-nitropyrimidine-2,4-diamine;
N2,N4-bis(lH-indol-5-ylmethyl)-5-nitropyrimidine-2,4-diamine;
N-(2 -methyl- lH-indol-5-yl)-2-chloro-pyrimidin-4-amine; N-(2-chloropyrimidin-4-yl)- 1 ,2-dimethyl- lH-indol-5-amine;
methyl 5-(2-chloropyrimidin-4-ylamino)- lH-indole-2-carboxylate;
N-(2-chloropyrimidin-4-yl)-2,3-dimethyl-lH-indol-5-amine;
N-(2-chloropyrimidin-4-yl)-lH-benzo[(i]imidazol-5-amine;
N-(2-chloropyrimidin-4-yl)-2-methyl-lH-benzo[(i]imidazol-5-amine;
2-chloro-6-methyl-N-[(2-methyl-lH-indol-5-yl)methyl]pyrimidin-4-amine;
2- [(tert-butyldimethylsilyloxy)methyl] -N-(2-chloropyrimidin-4-yl)- lH-indo 1-5 - amine;
N-(2-chloropyrimidin-4-yl)-N,2-dimethyl-lH-indol-5-amine;
N-(2-chloropyrimidin-4-yl)-N, 1 ,2-trimethyl- lH-indol-5-amine;
4-chloro-N-[2-(5-methoxy- 1 -methyl- lH-indol-3-yl)ethyl]pyrimidin-2-amine; and 4-chloro-N-[2-(5-methoxy- 1 -methyl- lH-indol-3-yl)ethyl]-N-methylpyrimidin-2- amine. These intermediate compounds may be used in processes for manufacturing compounds of formula I. Further, These intermediate compounds may be active as such, in therapy in general as well as in the uses and methods as set out in this specification.
Depending upon the substituents present in compounds of the formula I, the compounds may form esters, amides and/or salts which are within the scope of the present invention. Salts and solvates of compounds of formula I which are suitable for use in medicine are those wherein a counterion or an associated solvent is pharmaceutically acceptable. However, salts and solvates having non- pharmaceutically acceptable counterions or associated solvents are within the scope of the present invention, for example, for use as intermediates in the preparation of the compounds of formula I and their pharmaceutically acceptable salts, solvates and physiologically functional derivatives. By the term "physiologically functional derivative" is meant a chemical derivative of a compound of formula I having the same physiological function as the free compound of formula I, for example, by being convertible in the body thereto. Esters and amides are examples of physiologically functional derivatives.
A compound which, upon administration to the recipient, is capable of being converted into a compound of formula I as described above, or an active metabolite or residue thereof, is known as a "prodrug". A prodrug may, for example, be converted within the body, e. g. by hydrolysis in the blood, into its active form that has medical effects. Pharmaceutical acceptable prodrugs are described in T. Higuchi and V. Stella, Prodrugs as Novel Delivery Systems, Vol. 14 of the A. C. S.
Symposium Series (1976); "Design of Prodrugs" ed. H. Bundgaard, Elsevier, 1985; and in Edward B. Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987, which are incorporated herein by reference. Suitable salts according to the invention include those formed with organic or inorganic acids or bases. In particular, suitable salts formed with acids according to the invention include those formed with mineral acids, strong organic carboxylic acids, such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted, for example, by halogen, such as saturated or unsaturated dicarboxylic acids, such as hydroxycarboxyhc acids, such as amino acids, or with organic sulfonic acids, such as (Cj-C4)alkyl- or aryl-sulfonic acids which are unsubstituted or substituted, for example by halogen. Pharmaceutically acceptable acid addition salts include those formed from hydrochloric, hydrobromic, sulphuric, nitric, citric, tartaric, acetic, phosphoric, lactic, pyruvic, acetic, trifluoroacetic, succinic, perchloric, fumaric, maleic, glycolic, lactic, salicylic, oxaloacetic, methanesulfonic,
ethanesulfonic, p-toluenesulfonic, formic, benzoic, malonic, naphthalene-2-sulfonic, benzenesulfonic, isethionic, ascorbic, malic, phthalic, aspartic, and glutamic acids, lysine and arginine. Other acids such as oxalic, while not in themselves
pharmaceutically acceptable, may be useful as intermediates in obtaining the compounds of the invention and their pharmaceutical acceptable acid addition salts. Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts, for example those of potassium and sodium, alkaline earth metal salts, for example those of calcium and magnesium, and salts with organic bases, for example
dicyclohexylamine, N-methyl-D-glucamine, morpholine, thiomorpholine, piperidine, pyrrolidine, a mono-, di- or tri-lower alkylamine, for example ethyl-, tert-butyl-, diethyl-, diisopropyl-, triethyl-, tributyl- or dimethyl-propylamine, or a mono-, di- or trihydroxy lower alkylamine, for example mono-, di- or triethanolamine.
Corresponding internal salts may furthermore be formed. Those skilled in the art of organic chemistry will appreciate that many organic compounds can form complexes with solvents in which they are reacted or from which they are precipitated or crystallized. These complexes are known as
"solvates". For example, a complex with water is known as a "hydrate". The following definitions apply to the terms as used throughout this specification, unless otherwise limited in specific instances.
As used herein, the term "alkyl" means both straight and branched chain saturated hydrocarbon groups. Examples of alkyl groups include methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, and t-butyl groups. Among unbranched alkyl groups, there are preferred methyl, ethyl, n-propyl, and n-butyl groups. Among branched alkyl groups, there may be mentioned iso-propyl, iso-butyl, sec-butyl, and t-butyl groups. As used herein, the term "alkoxy" means the group O-alkyl, where "alkyl" is used as described above. Examples of alkoxy groups include, but are not limited to, methoxy and ethoxy groups. Other examples include propoxy and butoxy.
As used herein, the term "aryl" means a monocyclic or bicyclic aromatic carbocyclic group. Examples of aryl groups include phenyl and naphthyl. A naphthyl group may be attached through the 1 or the 2 position. In a bicyclic aromatic group, one of the rings may, for example, be partially saturated. Examples of such groups include indanyl and tetrahydronaphthyl. Specifically, the term (C6-Cio)aryl is used herein to mean a group comprising from 6 to 10 carbon atoms in a monocyclic or bicyclic aromatic group. A particularly preferred (C6-Cio)aryl group is phenyl.
As used herein, the term "halogen" means fluorine, chlorine, bromine or iodine. Fluorine, chlorine and bromine are particularly preferred.
As used herein, the term "heteroaryl" means an aromatic cyclic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen or sulfur. A heteroaryl group may, for example, be monocyclic, bicyclic or tricyclic.
Examples of monocyclic heteroaryl groups include, but are not limited to, furanyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, oxadiazolyl, thiadiazolyl, pyridyl, triazolyl, triazinyl, pyridazyl, iso thiazolyl, isoxazolyl, pyrazinyl, pyrazolyl, and pyrimidinyl.
Examples of bicyclic heteroaryl groups include, but are not limited to, quinoxalinyl, quinazolinyl, pyridopyrazinyl, benzoxazolyl, benzothiophenyl, benzimidazolyl, naphthyridinyl, quinolinyl, benzo furanyl, indolyl, indazolyl, benzothiazolyl, pyridopyrimidinyl, and isoquinolinyl.
Examples of tricyclic heteroaryl groups include, but are not limited to, carbazole, dibenzofuran, xanthene, and acridine.
As used herein, the term "heterocyclyl" means a cyclic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen and sulfur.
Examples of heterocyclyl groups include, but are not limited to, tetrahydrofuranyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, and dioxanyl. The compounds of the invention may be used in the prophylaxis and treatment as such, or preferably in a form of a pharmaceutical composition. While it is possible for the active ingredient to be administered alone, it is preferable for it to be present in a pharmaceutical formulation or composition. Accordingly, the invention provides a pharmaceutical formulation comprising a compound according to the invention, and a pharmaceutically acceptable diluent, excipient or carrier (collectively referred to herein as "carrier" materials). Pharmaceutical compositions of the invention may take the form of a pharmaceutical formulation as described below. Thus, the present invention relates to a pharmaceutical composition containing at least one compound of Formula I together with conventional excipients.
Exemplary compositions for oral administration include suspensions which can contain, for example, micro crystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer, and sweeteners or flavoring agents such as those known in the art; and immediate release tablets which can contain, for example, micro crystalline cellulose, dicalcium phosphate, starch, magnesium stearate, calcium sulfate, sorbitol, glucose and/or lactose and/or other excipients, binders, extenders, disintegrants, diluents and lubricants such as those known in the art. Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes and the like. Disintegrators include without limitation starch, methylcellulose, agar, bentonite, xanthan gum and the like. The compounds of formula I can also be delivered through the oral cavity by sublingual and/or buccal administration. Molded tablets, compressed tablets or freeze-dried tablets are exemplary forms which may be used. Exemplary compositions include those formulating the present compound(s) with fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins. Also included in such formulations may be high molecular weight excipients such as celluloses (avicel) or polyethylene glycols (PEG). Such formulations can also include an excipient to aid mucosal adhesion such as hydroxy propyl cellulose (HPC), hydroxy propyl methyl cellulose (HPMC), sodium carboxy methyl cellulose (SCMC), maleic anhydride copolymer (e.g., Gantrez), and agents to control release such as polyacrylic copolymer (e.g. Carbopol 934). Lubricants, glidants, flavors, coloring agents and stabilizers may also be added for ease of fabrication and use. Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like. For oral administration in liquid form, the oral drug components can be combined with any oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like.
The pharmaceutical formulations according to the invention include those suitable for oral, parenteral (including subcutaneous, intradermal, intramuscular, intravenous [bolus or infusion], and intraarticular), inhalation (including fine particle dusts or mists which may be generated by means of various types of metered dose pressurized aerosols), nebulizers or insufflators, rectal, intraperitoneal and topical (including dermal, buccal, sublingual, and intraocular) administration, although the most suitable route may depend upon, for example, the condition and disorder of the recipient.
Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, pills or tablets each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid, for example as elixirs, tinctures, suspensions or syrups; or as an oil-in-water liquid emulsion or a water-in- oil liquid emulsion. The active ingredient may also be presented as a bolus, electuary or paste.
A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, lubricating, surface active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein. The present compounds can, for example, be administered in a form suitable for immediate release or extended release. Immediate release or extended release can be achieved by the use of suitable pharmaceutical compositions comprising the present compounds, or, particularly in the case of extended release, by the use of devices such as subcutaneous implants or osmotic pumps. The present compounds can also be administered liposomally.
Preferred unit dosage formulations are those containing an effective dose, as hereinbefore recited, or an appropriate fraction thereof, of the active ingredient.
It should be understood that in addition to the ingredients particularly mentioned above, the formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents.
The formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing the active ingredient into association with the carrier which constitutes one or more accessory ingredients. In general the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.
The compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, 1,2-dipalmitoylphosphatidylcholine, phosphatidyl ethanolamine (cephaline), phosphatidylserine, phosphatidylinositol, diphosphatidylglycerol (cardiolipin) or phosphatidylcholine (lecithin).
Formulations for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze- dried (lyophilised) condition requiring only the addition of the sterile liquid carrier, for example saline or water-for-injection, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described. Exemplary compositions for parenteral administration include injectable solutions or suspensions which can contain, for example, suitable non-toxic, parenterally acceptable diluents or solvents, such as polyethylene glycol, ethanol, 1,3-butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid, or Cremaphor.
Exemplary compositions for nasal, aerosol or inhalation administration include solutions in saline, which can contain, for example, benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, and/or other solubilizing or dispersing agents such as those known in the art.
Formulations for rectal administration may be presented as a suppository with the usual carriers such as cocoa butter, synthetic glyceride esters or polyethylene glycol. Such carriers are typically solid at ordinary temperatures, but liquefy and/or dissolve in the rectal cavity to release the drug. Formulations for topical administration in the mouth, for example buccally or sublingually, include lozenges comprising the active ingredient in a flavoured basis such as sucrose and acacia or tragacanth, and pastilles comprising the active ingredient in a basis such as gelatin and glycerine or sucrose and acacia. Exemplary compositions for topical administration include a topical carrier such as Plastibase (mineral oil gelled with polyethylene).
The amount of active ingredient which is required to achieve a therapeutic effect will, of course, vary with the particular compound, the route of administration, the subject under treatment, including the type, species, age, weight, sex, and medical condition of the subject and the renal and hepatic function of the subject, and the particular disorder or disease being treated, as well as its severity. An ordinarily skilled physician, veterinarian or clinician can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.
Oral dosages of the present invention, when used for the indicated effects, will range between about 0.01 mg per kg of body weight per day (mg/kg/day) to about 100 mg/kg/day, preferably 0.01 mg per kg of body weight per day (mg/kg/day) to 10 mg/kg/day, and most preferably 0.1 to 5.0 mg/kg/day, for adult humans. For oral administration, the compositions are preferably provided in the form of tablets or other forms of presentation provided in discrete units containing 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 5.0, 10.0, 15.0, 25.0, 50.0, 100, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated. A medicament typically contains from about 0.01 mg to about 500 mg of the active ingredient, preferably from about 1 mg to about 100 mg of active ingredient.
Intravenously, the most preferred doses will range from about 0.1 to about 10 mg/kg/minute during a constant rate infusion. Advantageously, compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily. Furthermore, preferred compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using those forms of transdermal skin patches well known to those of ordinary skill in the art. To be administered in the form of a transdermal delivery system, the dosage
administration will, of course, be continuous rather than intermittent throughout the dosage regimen.
The invention also provides the use of a compound of formula I, for the manufacture of a medicament for the treatment or prophylaxis of cancer.
The compounds and the pharmaceutical compositions of the invention may be used in the prophylaxis and treatment of diseases such as cancer, diseases caused by parasites, allergic diseases, Crohns disease, rheumatic diseases, tuberculosis, diabetes, Alzheimer's disease, inflammatory diseases, multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), Parkinson's disease and diseases caused by bacteria, viruses and fungus.
The compounds of the invention find particular application in the treatment or prophylaxis of various cancer types, including but not limited to, cancer of the bone, breast, respiratory tract, brain, reproductive organs, bone marrow, digestive tract, urinary tract, eye, liver, skin, head, neck, thyroid, parathyroid, and metastatic forms thereof. Proliferative disorders of the breast include, but are not limited to, invasive ductal carcinoma, invasive lobular carcinoma, ductal carcinoma, lobular carcinoma in situ, and metastatic breast cancer. Proliferative disorders of the skin include, but are not limited to, basal cell carcinoma, squamous cell carcinoma, malignant melanoma, and Kaposi's sarcoma. Proliferative disorders of the respiratory tract include, but are not limited to, lung cancer, doxorubicin resistant lung cancer, small cell and non- small cell lung carcinoma, bronchial adenoma, pleuropulmonary blastoma, and malignant mesothelioma. Proliferative disorders of the brain include, but are not limited to, brain stem and hypothalamic glioma, cerebellar and cerebral astrocytoma, medulloblastoma, ependymal tumors, oligodendroglial tumors, meningiomas and neuroectodermal, and pineal tumors. Proliferative disorders of the male reproductive organs include, but are not limited to, prostate, testicular and penis cancer. Proliferative disorders of the female reproductive organs include, but are not limited to, uterine, cervical, ovarian, vaginal, vulval cancer, uterine sarcoma, ovarian germ cell tumor, ovarian cancer, doxorubicin resistant ovarian cancer, and cisplatin resistant ovarian cancer. Proliferative disorders of the digestive tract include, but are not limited to, anal, colon, colorectal, esophageal, gall bladder, stomach, pancreatic, rectal, small intestine, and salivary gland cancer. Proliferative disorders of the liver include, but are not limited to, hepatocellular carcinoma, cholangiocarcinoma, and primary liver cancer. Proliferative disorders of the eye include, but are not limited to, intraocular melanoma, retinoblastoma, and rhabdomyosarcoma. Proliferative disorders of the head include, but are not limited to, laryngeal, hypopharyngeal, nasopharyngeal, oropharyngeal, lip, oral, and metastatic paranasal sinus cancer.
Proliferative disorders of the lymphomas include, but are not limited to, T cell and B cell lymphomas, non-Hodgkins lymphoma, cutaneous T cell lymphoma, Hodgkins disease, vincristin resistant lymphoma, and lymphoma of the central nervous system. Leukaemia includes, but is not limited to, acute myeloid leukaemia, chronic myelogenous leukaemia, acute lymphocytic leukaemia, chronic lymphocytic leukaemia, teponiside resistant leukaemia, and hairy cell leukaemia. Proliferative disorders of the thyroid include, but are not limited to, thyroid cancer, thymoma, and malignant thymoma. Proliferative disorders of bone marrow include, but are not limited to, myeloma, and doxorubicin resistant myeloma. Proliferative disorders of the urinary tract include, but are not limited to, kidney cancer and bladder cancer. Sarcomas include, but are not limited to, sarcoma of the soft tissue, osteosarcoma, malignant fibrous histiocytoma, lymphosarcoma, and rhabdomyosarcoma.
Whilst a compound of the invention may be used alone, it is also possible for the compounds to be used in combination with each other, in combination with radiation therapy, or in combination with other anticancer agents. Various classes of anticancer and antineoplastic compounds include, but are not limited to, alkylating agents, antimetabolites, anticancer camptothecin derivatives, plant-derived anticancer agents, antibiotics, enzymes, platinum coordination complexes, tyrosine kinase inhibitors, hormones and hormone antagonists, monoclonal antibodies, interferons, biological response modifiers and other anticancer agents. Examples of alkylating agents include, but are not limited to, mechlorethamine, cyclophosphamide, ifosfamide, melphalan, chlorambucil, busulfan, mitobronitol, ranimustin, nimustin, temozolomide and carmustine; examples of antimetabolites include, but are not limited to, methotrexate, fluorouracil, cytarabine, gemcitabine, fludarabine, mercaptopurine, thioguanine, and azathioprine; examples of camptothecin derivatives include, but are not limited to, irinotecan, topotecan, and camptothecin; examples of plant-derived agents include, but are not limited to, vinca alkaloids e.g. vinblastine and vincristine, taxanes, e.g. paclitaxel and docetaxel, and colchicines; examples of antibiotics include, but are not limited to, actinomycin D, daunorubicin, and bleomycin. One example of enzyme effective as antineoplastic agent includes L-asparaginase.
Examples of coordination compounds include, but are not limited to, cisplatin and carboplatin; examples of tyrosine kinase inhibitors include, but are not limited to, gefitinib, imatinib, sunitinib, nilotinib, dasatinib, erlotinib, and pazopanib; examples of hormones and hormone related compounds include, but are not limited to, prednisone, dexamethasone, formestane, aminoglutethimide, anastrozole,
hydroxyprogesterone caproate, medroxyprogesterone and tamoxifen; examples of interferons include, but are not limited to, interferon a, interferon a-2a, interferon a- 2b, interferon β, interferon γ-la, and interferon γ-ηΐ; examples of biological response modifiers include, but are not limited to, krestin, lentinan, sizofiran, picibanil, and ubenimex. Examples of other anticancer agents include, but are not limited to, mitoxantrone, procarbazine, dacarbazine, hydroxycarbamide, pentostatin, tretinoin, leuprorelin, flutamide, and aldesleukin.
Numerous synthetic routes to the compounds of the present invention can be devised by any person skilled in the art and the possible synthetic routes described below do not limit the invention.
Procedure for synthesizing the compounds of general formula I
The appropriate amine (II) was dissolved in z'so-propanol (0.2 g/mL). 1.1 Eq of pyrimidine (III) and 1.2 eq of N,N-diisopropylethylamine (DIPEA) were added and the mixture was stirred at 50-120 °C for 1-3 h. The reaction mixture was dissolved in EtOAc/MeOH 5: 1 and washed with saturated aqueous NaHC03, water and brine. The solvent was removed in vacuo and the residue was purified by column
chromatography on silica gel with heptane/EtOAc or EtOAc/MeOH as eluent to give the major intermediate (IV) and the minor intermediate (IV"). The intermediate (IV or IV") was dissolved in ethylene glycol (0.2 g/mL) and 1.1 eq of amine (V) was added. The mixture was then stirred at 100-150 °C for 1-3 h. The reaction mixture was dissolved in EtOAc/MeOH 5: 1 and washed with saturated aqueous NaHC03, water and brine. The solvent was removed in vacuo and the residue was purified by column chromatography on silica gel with heptane/EtOAc or EtOAc/MeOH/TEA as eluent to give the compound of formula I. This procedure is exemplified in Scheme 1.
Scheme 1
Figure imgf000057_0001
Compounds of Examples 1 - 22, 24, 26 - 28, 30 - 37, 62, 64, 66, 68, 69, 71 - 91, 97 - 103, and 109 were synthesized by this general reaction procedure (as set out in Scheme 1.). Note that Example 102 was obtained by a final ester hydrolysis step using potassium hydroxide in methanol. A, D, E, R1 to R5, and L are as defined in formula I. General procedure for synthesizing the compounds of general formula I, represented by formula VII, starting from compounds of formula VI
The intermediate VI which was synthesized by the general procedure in Scheme 1 was dissolved in MeOH (1.5 mg/mL) and 10 % Pd/C (20 mol %) was added. The flask was pump-purged with argon and then with hydrogen. The reaction mixture was left stirring vigorously under hydrogen atmosphere at room temperature for 20 h. The catalyst was filtered off and the solvent was evaporated yielding pure product VII, as exemplified in Scheme 2.
Scheme 2
Figure imgf000058_0001
VI VII
Compounds of Examples 38 - 41 were synthesized by this general reaction procedure (as set out in Scheme 2.). A, D, E, R1, R4, R5, and L are as defined in formula I.
Alternative procedure for synthesizing the compounds of general formula I In some cases appropriate amines were introduced in reversed order to give the compounds of formula I more efficiently via intermediate VIII. The conditions for this procedure resembles the one described for Scheme 1 and are exemplified in Scheme 3.
Figure imgf000059_0001
VIII"
The procedure described in scheme 3 was utilized in the syntheses of Examples 23, 25, 29, 63, 65, 67, 70, 92 - 96, and 104 - 108. Note that Example 104 was obtained by a final desilylation step by the action of tetrabutylammonium fluoride. A, D, E, R1 to R5, and L are as defined in formula I.
Procedure for synthesizing the intermediates of general formula IX where R5 = Me and/or R6 = Me
In order to access certain methylated analogues the appropriate mono substituted pyrimidine (VIII) was dissolved in dimethylformamide (0.1 g/mL). 2 Eq of CS2CO3 and 2 eq of iodomethane were added and the mixture was stirred at 20-40 °C for 2-5 days. The reaction mixture was dissolved in EtOAc and washed with water. The solvent was removed in vacuo and the residue was purified by column
chromatography on silica gel with heptane/EtOAc as eluent to give the compound (IX). The procedure is exemplified in Scheme 4.
The monomethylated compound IX'a (Intermediate 117) was obtained as a major product under these reaction conditions and used in the synthesis of Example 105. A minor component was obtained due to an additional methylation at the indole nitrogen. This dimethylated compound IX'b (Intermediate 118) was used in the synthesis of Example 106. Under the same reaction conditions, monomethylation at the indole nitrogen yielded IX"a (Intermediate 119) that was used in the synthesis of Example 107. As a minor product, the dimethylated compound IX"b (Intermediate 120) was obtained and used in the synthesis of Example 108.
Scheme 4
Figure imgf000060_0001
Intermediate compounds 117 - 120 were synthesized by this reaction procedure (as set out in scheme 4). R1 - R3 are as defined in formula I.
Procedure for synthesizing alkylated serotonin derivatives of formula XIII, used in the syntheses of Examples 73, 74, and 87
Step 1 : Serotonin hydrochloride (X) was dissolved in water (20 mg/mL). 3 Eq of potassium carbonate and 1 eq of di-tert-butyl dicarbonate were added and the mixture was stirred at room temperature for 24 h. The aqueous reaction mixture was extracted with EtOAc and the organic phase was washed with water, 1 M HC1 (aq) and brine. The solvent was removed in vacuo and the residue was purified by column chromatography on silica gel with Cf^CVMeOH as eluent to give tert-butyl 2-(5- hydroxy- 1 H-indo 1-3 -yl)ethylcarbamate (XI) .
Step 2: 7¾rt-butyl 2-(5 -hydroxy- 1 H-indo 1-3 -yl)ethylcarbamate (XI), 3-9 eq potassium carbonate, and 0.1-1 eq Nal were premixed in 2-butanone (25 mg/mL). After 5 min, 3-5 eq alkyl halide (R'-X = 4-(2-chloroethyl)morpholine*HCl or 2-bromoethyl methyl ether or bromoethane) was added and the mixture was stirred at 90 °C for 3-5 days. The reaction mixture was dissolved in EtOAc and washed with saturated aqueous NaHCC"3. The solvent was removed in vacuo and the residue was purified by column chromatography on silica gel with Ct^CVacetone to give the alkylated derivative (XII).
Step 3: The alkylated derivative (XII) was dissolved in Ct^Cytrifluoroacetic acid 2: 1 (25 mg/mL) and kept at room temperature for 30-90 min. The reaction mixture was concentrated in vacuo, co-evaporated with toluene and the residue was purified by column chromatography on silica gel with EtOAc/MeOH/TEA as eluent to give the desired amine (XIII).
This procedure is exemplified in Scheme 4.
Scheme 4
Figure imgf000061_0001
XIIo and XIIIo Alkylated serotonin derivatives (XHIa was used in the synthesis of Example 73, XHIb was used in the synthesis of Example 74, and XIIIc was used in the synthesis of Example 87) were synthesized by this reaction procedure (as set out in Scheme 4.). Below follows non-limiting examples of the invention.
Example 1
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4-diamine
Figure imgf000062_0001
1H NMR (500 MHz, DMSO-d6) ^ 11.00 (s, 1H), 10.95 (s, 1H), 8.33 (s, 1H), 7.85 (d, 1H), 7.81 (d, 1H), 7.58 (br s, 1H), 7.49 (s, 1H), 7.34 (d, 1H), 7.30 (t, 1H), 7.19 (t, 1H), 7.09 (d, 1H), 6.99 (m, 2H), 6.80 (m, 1H), 6.36 (m, 1H), 5.98 (d, 1H), 4.61 (br s, 2H).
MS (ESI+) mlz 355.3 [M + H]+.
Example 2
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)pyrimidine-2,4-diamine
Figure imgf000062_0002
1H NMR (500 MHz, DMSO- 6) ^ 11.00 (s, 1H), 10.80 (s, 1H), 8.60 (s, 1H), 8.00 (m, 1H), 7.76 (d, 1H), 7.50 (s, 1H), 7.49 (br s, 1H), 7.34-7.28 (m, 3H), 7.22-7.19 (m, 2H), 7.10 (m, 1H), 6.35 (m, 1H), 6.27 (m, 1H), 5.91 (d, 1H), 4.61 (br s, 2H).
MS (ESI+) mlz 355.3 [M + H]+. Example 3 N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-yl)pyrimidin
Figure imgf000063_0001
1H-NMR (500 MHz, DMSO-d6) δ 10.99 (s, IH), 10.60 (s, IH), 8.53 (s, IH), 7.84 (d, IH), 7.75 (d, IH), 7.50 (s, IH), 7.46 (br. s, IH), 7.33 (d, IH), 7.29 (t, IH), 7.21 (dd, IH), 7.10 (dd, IH), 7.07 (d, IH), 6.35 (br. s, IH), 5.96 (s, IH), 5.89 (d, IH), 4.59 (br. s, 2H), 2.33 (s, 3H).
MS (ESI+) mlz 369.3 [M + H]+.
Example 4
N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-5-yl)pyrimidine-2,4-diamine
Figure imgf000063_0002
1H-NMR (500 MHz, CD3OD) δ 8.01 (s, IH), 7.80 (br. s, IH), 7.72 (d, IH), 7.51 (s, IH), 7.43 (dd, IH), 7.39 (d, IH), 7.33 (d, IH), 7.19 (d, IH), 7.10 (dd, IH), 6.37 (d, IH), 5.97 (d, IH), 4.64 (br. s, 2H).
MS (ESI+) mlz 356.3 [M + H]+.
Example 5
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-ylmethyl)pyrimidine-2^-di^
Figure imgf000063_0003
1H NMR (500 MHz, DMSO-d6) δ 10.99 (s, IH), 10.74 (s, IH), 7.61 (d, IH), 7.45 (s, IH), 7.30-7.28 (m, 3H), 7.25 (br s, IH), 7.13 (d, IH), 7.05 (dd, IH), 6.96 (dd, IH), 6.75 (br s, IH), 6.34 (br s, IH), 5.99 (br s, IH), 5.72 (d, IH), 4.51 (br s, 2H), 4.46 (d,
2H), 2.34 (s, 3H).
MS (ESI+) mlz 383.3 [M + H]+. Example 6
N2-(lH-indazol-5-ylmethyl)-N4-(lH-indol-5-ylmethyl)pyrimidine-2,4-diamine
Figure imgf000064_0001
1H NMR (500 MHz, DMSO-d6) δ 12.92 (s, IH), 10.99 (s, IH), 7.92 (br s, IH), 7.61 (d, IH), 7.59 (s, IH), 7.43-7.39 (m, 2H), 7.33-7.28 (m, 4H), 7.02 (d, IH), 6.95 (br s, IH), 6.32 (s, IH), 5.74 (d, IH), 4.51 (d, 4H).
MS (ESI+) mlz 370.3 [M + H]+.
N2-nH-benzo[ ]imidazol-5-ylmethyl)-^
diamine
Figure imgf000064_0002
1H NMR (500 MHz, DMSO-d6, 75°C) <5 12.10 (br s, IH), 10.83 (br s, IH), 8.10 (s, IH), 7.65 (s, IH), 7.54 (s, IH), 7.48-7.46 (m, 3H), 7.30 (d, IH), 7.27 (t, IH), 7.18 (d, IH), 7.04 (d, IH), 7.00 (br s, IH), 6.34 (br s, IH), 5.85 (d, IH), 4.60 (d, 2H), 4.54 (d, 2H).
MS (ESI+) mlz 370.2 [M + H]+.
Example 8
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-6-ylmethyl)pyrimidine-2,4-diamine
Figure imgf000065_0001
1H NMR (500 MHz, DMSO-d6) δ 10.98 (s, IH), 10.93 (s, IH), 7.62 (d, IH), 7.44 (s, IH), 7.40 (d, IH), 7.32 (s, IH), 7.29-7.24 (m, 4H), 7.03 (d, IH), 6.96 (d, IH), 6.88 (br s, IH), 6.35 (m, IH), 6.32 (br s, IH), 5.73 (d, IH), 4.52 (d, 2H), 4.50 (br s, 2H).
MS (ESI+) mlz 369.3 [M + H]+.
Example 9
N2-[2-(lH-indol-3-yl)ethyll-N4-(lH-indol-5-ylmethyl)pyrimidine-2^
Figure imgf000065_0002
1H-NMR (300 MHz, CDC13) δ 8.20 (br s, IH), 7.95 (br s, IH), 7.83 (d, IH), 7.65 (d, IH), 7.60 (s, IH), 7.38 - 6.91 (m, 7H), 6.53 (br s, IH), 5.73 (d, IH), 4.94 (m, 2H), 4.58 (m, 2H), 3.73 (q, 2H), 3.06 (t, 2H).
MS (ESI+) mlz 383.3 [M + H]+. Example 10
3-(2-[4-(lH-indol-5-ylmethylamino)-pyrimidin-2-ylaminolethyl|-lH-indol-5-ol
Figure imgf000065_0003
1H-NMR (500 MHz, DMSO-d6) δ 10.99 (s, IH), 10.44 (s, IH), 8.56 (s, IH), 7.65 (br. s, IH), 7.47 (s, IH), 7.31 (d, IH), 7.29 (t, IH), 7.25 (br. s, IH), 7.11 (d, IH), 7.06 (d, IH), 7.02 (s, IH), 6.87 (d, IH), 6.58 (dd, IH), 6.34 (br. s, 2H), 5.75 (br. s, IH), 4.52 (br. s, 2H), 3.46 (q, 2H), 2.81 (t, 2H).
MS (ESI+) mlz 399.3 [M + H]+. Example 11
A^-(lH-indol-5-ylmethyl -N2 2-(5-methyl H-indol-3-vnethyl1pyrimidine-2,4- diamine
Figure imgf000066_0001
1H-NMR (500 MHz, DMSO-d6) δ 10.99 (s, 1H), 10.62 (s, 1H), 7.64 (br. s, 1H), 7.47 (s, 1H), 7.33 (s, 1H), 7.31 (d, 1H), 7.29 (t, 1H), 7.26 (br. s, 1H), 7.19 (d, 1H), 7.08 (s, 1H), 7.06 (d, 1H), 6.86 (d, 1H), 6.37 (br. s, 1H), 6.34 (br. s, 1H), 5.74 (dd, 1H), 4.54 (br. s, 2H), 3.48 (q, 2H), 2.88 (t, 2H), 2.32 (s, 3H).
MS (ESI+) m/z 397.3 [M + H]+.
Example 12
A^-(lH-indol-5-ylmethyl -N2-r2-(5-methoxy-lH-indol-3-vnethyl1pyrimidine-2,4- diamine
Figure imgf000066_0002
1H-NMR (500 MHz, DMSO-d6) δ 10.99 (s, 1H), 10.60 (s, 1H), 7.65 (br. s, 1H), 7.47 (s, 1H), 7.31 (d, 1H), 7.29 (t, 1H), 7.26 (br. s, 1H), 7.20 (d, 1H), 7.10-7.06 (m, 3H), 6.69 (dd, 1H), 6.37 (br. s, 1H), 6.34 (br. s, 1H), 5.75 (s, 1H), 4.53 (br. s, 2H), 3.71 (s, 3H), 3.48 (q, 2H), 2.88 (t, 2H).
MS (ESI+) m/z 413.3 [M + H]+.
Example 13
N2-(lH-indol-4-yl)-N4-(2 -methyl- lH-indol-5-ylmethyl)pyrimidine-2,4-diamine
Figure imgf000066_0003
1H-NMR (500 MHz, DMSO-dg) δ 10.95 (s, IH), 10.81 (s, IH), 8.32 (s, IH), 7.86 (d, IH), 7.80 (d, IH), 7.54 (br s, IH), 7.35 (s, IH), 7.19 (d, IH), 7.18 (s, IH), 6.97 (m, 3H), 6.80 (s, IH), 6.05 (s, IH), 5.98 (d, IH), 4.58 (br s, 2H), 2.35 (s, 3H).
MS (ESI+) m/z 369.3 [M + H]+.
Example 14
N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-ylmethyl)pyrimidine-2^-dk
Figure imgf000067_0001
1H-NMR (500 MHz, DMSO-d6) δ 10.95 (s, IH), 10.79 (s, IH), 7.61 (d, IH), 7.44 (s, IH), 7.30 (s, IH), 7.26 (dd, 2H), 7.23 (br. s, IH), 7.15 (d, IH), 7.06 (d, IH), 6.94 (d, IH), 6.79 (br.s, IH), 6.32 (d, IH), 6.01 (d, IH), 5.72 (d, IH), 4.49 (d, 4H), 2.35 (s, 3H).
MS (ESI+) m/z 383.3 [M + H]+. Example 15
N2,N4-Bis-(2-methyl-lH-indol-5-ylmethyl)pyrimidine-2,4-diamine
Figure imgf000067_0002
1 H-NMR (500 MHz, DMSO-d6/D20, 75°C) δ 7.63 (d, IH), 7.32 (s, 2H), 7.17 (d, IH), 7.14 (d, IH), 6.97 (t, 2H), 6.01 (d, 2H), 5.75 (d, IH), 4.49 (s, 4H), 2.36 (s, 6H).
MS (ESI+) m/z 397.4 [M + H]+.
Example 16
N2-(lH-indazol-5-ylmethyl)-N4-(2 -methyl- lH-indol-5-ylmethyl)pyrimidine-2,4- diamine
Figure imgf000068_0001
1 H-NMR (500 MHz, CD3OD) δ 7.85 (br s, 1H), 7.63 (s, 1H), 7.58 (br s, 1H), 7.41 (d, 1H), 7.36 (d, 1H), 7.29 (s, 1H), 7.11 (d, 1H), 6.91 (d, 1H), 5.97 (s, 1H), 5.83 (d, 1H), 4.64 (s, 2H), 4.59 (br s, 2H), 2.38 (s, 3H).
MS (ESI+) mlz 384.3 [M + H]+.
Example 17
N2-(2-(lH-indol-3-yl -ethyl -N4-(2-methyl-lH-indol-5-ylmethvnpyrimidine-2,4- diamine
Figure imgf000068_0002
1 H-NMR (500 MHz, DMSO-d6) δ 10.80 (s, 1H), 10.76 (s, 1H), 7.64 (br s, 1H), 7.55 (d, 1H), 7.32 (s, 1H), 7.31 (s, 1H), 7.25 (br s, 1H), 7.18 (d, 1H), 7.14 (s, 1H), 7.04 (t, 1H), 6.97 (d, 1H), 6.92 (br s, 1H), 6.39 (br s, 1H), 6.02 (s, 1H), 5.74 (s, 1H), 4.51 (br s, 2H), 3.45 (q, 2H), 2.90 (t, 2H), 2.34 (s, 3H).
MS (ESI+) mlz 397.3 [M + H]+.
Example 18
N2-(lH-indol-4-yl)-N4-(lH-indazol-5-ylmethyl)pyrimidine-2,4-diamine
Figure imgf000068_0003
1 H-NMR (500 MHz, DMSO-d6) δ 12.97 (s, 1H), 10.95 (s, 1H), 8.36 (s, 1H), 7.99 (s, 1H), 7.82 (d, 1H), 7.75 (br s, 1H), 7.68 (br s, 1H), 7.65 (s, 1H), 7.48 (d, 1H), 7.35 (d, 1H), 7.19 (s, 1H), 6.98 (d, 1H), 6.92 (t, 1H), 6.77 (s, 1H), 5.99 (br s, 1H), 4.62 (br s, 2H). MS (ESI+) mlz 356.3 [M + H]+. Example 19
N4-(lH-benzo[ imidazol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimid^
Figure imgf000069_0001
1H NMR (500 MHz, DMSO-d6, 75°C) <5 12.16 (br s, 1H), 10.79 (s, 1H), 8.10 (s, 1H), 8.03 (s, 1H), 7.83 (d, 1H), 7.78 (d, 1H), 7.54 (br s, 2H), 7.44 (m, 1H), 7.20 (m, 1H), 7.18 (t, 1H), 7.00 (d, 1H), 6.94 (t, 1H), 6.72 (br s, 1H), 6.02 (d, 1H), 4.66 (d, 2H). MS (ESI+) mlz 356.2 [M + H]+.
Example 20
N4-(lH-indol-6-ylmethyl)-N -(lH-indol-4-yl)pyrimidine-2,4-diamine
Figure imgf000069_0002
1H NMR (500 MHz, DMSO-dg) δ 11.00 (s, 1H), 10.94 (s, 1H), 8.34 (s, 1H), 7.83 (m, 2H), 7.63 (br s, 1H), 7.48 (d, 1H), 7.36 (s, 1H), 7.28 (t, 1H), 7.18 (t, 1H), 7.01 (dd, 1H), 6.98-6.91 (2H), 6.80 (m, 1H), 6.37 (m, 1H), 6.00 (br s, 1H), 4.65 (br s, 2H). MS (ESI+) mlz 355.3 [M + H]+.
Example 21
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-6-ylmethyl)pyrimidine-2,4-diamine
Figure imgf000069_0003
1H NMR (500 MHz, DMSO-d6) δ 10.97 (s, IH), 10.94 (s, IH), 7.63 (d, IH), 7.44 (m, 2H), 7.31-7.23 (m, 5H), 7.05 (dd, IH), 6.95 (dd, IH), 6.79 (br s, IH), 6.37 (m, IH), 6.30 (br s, IH), 5.74 (m, IH), 4.55 (br s, 2H), 4.48 (d, 2H).
MS (ESI+) m/z 369.3 [M + H]+.
Example 22
N2,A/4-bis-(lH-indol-6-ylmethyl)pyrimidine-2,4-diamine
Figure imgf000070_0001
1H NMR (500 MHz, DMSO-d6) δ 10.96 (s, IH), 10.92 (s, IH), 7.63 (d, IH), 7.43 (d, IH), 7.39 (d, IH), 7.31 (m, 3H), 7.27 (t, IH), 7.25 (t, IH), 6.95 (m, 2H), 6.87 (br s, IH), 6.36 (m, IH), 6.34 (m, IH), 5.75 (m, IH), 4.53 (br s, 2H), 4.51 (d, 2H).
MS (ESI+) m/z 369.3 [M + H]+.
Example 23
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-4-yl)pyrimidine-2,4-diamine
Figure imgf000070_0002
1H-NMR (500 MHz, DMSO-d6): δ 11.05 (br s, IH), 10.94 (br s, IH), 8.70 (s, IH), 7.82 (d, IH), 7.72 (br s, IH), 7.46 (s, IH), 7.29-7.25 (m, 3H), 7.12-7.02 (m, 3H), 6.95 (t, IH), 6.67 (s, IH), 6.33 (s, IH), 6.15 (d, IH), 4.54 (d, 2H).
MS (ESI+) m/z 355.3 [M + H]+.
Example 24
N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2^-diamine
Figure imgf000071_0001
1 H-NMR (500 MHz, DMSO-d6) δ 10.95 (s, IH), 10.73 (s, IH), 8.74 (s, IH), 7.74 (d, IH), 7.70 (s, IH), 7.47 (s, IH), 7.29 (d, IH), 7.27-7.25 (m, IH), 7.14 (d, IH), 7.10 (d, IH), 7.06 (d, IH), 7.00 (br. s, IH), 6.33 (s, IH), 6.01 (s, IH), 5.88 (d, IH), 4.53 (d, 2H), 2.35 (s, 3H).
MS (ESI+) m/z 369.3 [M + H]+.
Example 25
N4-[2-(lH-indol-3-yl)ethyll-N2-(lH-indol-5-ylmethyl)pyrimidine-2^-dk
Figure imgf000071_0002
1H NMR (500 MHz, DMSO-d6) δ 10.95 (s, IH), 10.80 (s, IH), 7.62 (br s, IH), 7.51 (d, IH), 7.45 (s, IH), 7.33-7.26 (m, 3H), 7.14 (s, IH), 7.08-6.92 (m, 4H), 6.81 (br s, IH), 6.31 (s, IH), 5.69 (m, IH), 4.51 (d, 2H), 3.52 (m, 2H), 2.90 (t, 2H).
MS (ESI+) m/z 383.3 [M + H]+.
Example 26
3-(2-[2-(lH-indol-5-ylmethylamino)-pyrimidin-4-ylaminolethyl|-lH-indol-5-ol
Figure imgf000071_0003
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.75 (s, IH), 10.28 (s, IH), 8.32 (br. s, IH), 7.62 (d, IH), 7.48 (s, IH), 7.28 (d, IH), 7.24-7.23 (m, IH), 7.12 (d, IH), 7.09 (d, IH), 7.02 (s, IH), 6.87 (d, IH), 6.62 (br. s, IH), 6.60 (dd, IH), 6.41 (br. s, IH), 6.33 (s, IH), 5.73 (d, IH), 4.54 (d, 2H), 3.50 (q, 2H), 2.85 (t, 2H).
MS (ESI+) mlz 399.2 [M + H]+. Example 27
N2-(lH-indol-5-ylmethyl -A^-r2-(5-methyl H-indol-3-yl ethyl1pyrimidine-2,4- diamine
Figure imgf000072_0001
1H-NMR (500 MHz, DMSO-d6) δ 10.93 (s, IH), 10.65 (s, IH), 7.62 (br. s, IH), 7.46 (s, IH), 7.30 (s, IH), 7.27-7.25 (m, 2H), 7.20 (d, IH), 7.10-7.06 (m, 2H), 6.93 (br. s, IH), 6.87 (d, IH), 6.77 (br. s, IH), 6.30 (s, IH), 5.70 (d, IH), 4.51 (d, 2H), 3.51 (br. s, 2H), 2.88 (t, 2H), 2.33 (s, 3H).
MS (ESI+) mlz 397.3 [M + H]+.
Example 28
N2-(lH-indol-5-ylmethyl -A^-r2-(5-methoxy-lH-indol-3-vnethyl1pyrimidine-2,4- diamine
Figure imgf000072_0002
1H-NMR (500 MHz, DMSO-d6) δ 10.93 (s, IH), 10.63 (s, IH), 7.62 (br. s, IH), 7.45 (s, IH), 7.28-7.25 (m, 2H), 7.21 (d, IH), 7.09 (s, IH), 7.07 (d, IH), 6.98 (d, IH), 6.93 (br. s, IH), 6.74 (br. s, IH), 6.69 (dd, IH), 6.30 (s, IH), 5.70 (d, IH), 4.50 (d, 2H), 3.69 (s, 3H), 3.51 (br. s, 2H), 2.88 (t, 2H).
MS (ESI+) mlz 413.3 [M + H]+.
Example 29
N2-(lH-indazol-5-ylmethyl)-N4-(lH-indol-4-yl)pyrimidine-2,4-diamine
Figure imgf000073_0001
1 H-NMR (300 MHz, DMSO-d6) δ 12.94 (br s, 1H), 11.07 (br s, 1H), 8.74 (s, 1 H), 7.97 (s, 1H), 7.82 (d, 1H), 7.68 (m, 1H), 7.61 (s, 1H), 7.45 (d, 1H), 7.35 (d, 1H), 7.25 (m, 2H), 7.06 (d, 1H), 6.96 (m, 1H), 6.66 (s, 1H), 6.16 (d, 1H), 4.55 (d, 2H).
MS (ESI+) m/z 356.3 [M + H]+.
Example 30
N2-(lH-indol-4-yl)-N4-(lH-indol-5ylmethyl)-6-methylpyrimidine-2^-dia
Figure imgf000073_0002
1 H-NMR (500 MHz, DMSO-d6) δ 10.99 (s, 1H), 10.93 (s, 1H), 8.26 (s, 1H), 7.90 (br s, 1H), 7.48 (s, 1H), 7.45 (br s, 1H), 7.33 (d, 1H), 7.29 (s, 1H), 7.18 (s, 1H), 7.08 (d, 1H), 6.94 (m, 2H), 6.82 (s, 1H), 6.36 (s, 1H), 5.85 (s, 1H), 4.59 (br s, 2H), 2.12 (s, 3H).
MS (ESI+) m/z 369.3 [M + H]+.
Example 31
N2,N4-bis(lH-indol-5-ylmethyl)-6-methylpyrimidine-2,4-diamine
Figure imgf000073_0003
1 H-NMR (300 MHz, CD3OD) δ 7.49 (d, 2H), 7.28 (d, 2H), 7.19 (d, 2H), 7.07 (dt, 2H), 6.35 (d, 2H), 5.71 (s, 1H), 4.62-4.60 (m, 4H), 2.11 (s, 3H).
MS (ESI+) mlz 383.3 [M + H]+. Example 32
3-(2-[4-nH-indol-5-ylmethylamin^
indol-5-ol
Figure imgf000074_0001
1H-NMR (300 MHz, CDC13+CD30D) δ 7.51 (s, IH), 7.32 (d, IH), 7.18 (d, IH), 7.14 (d, IH), 7.08 (d, IH), 6.97-6.89 (m, 2H), 6.69 (dd, IH), 6.43 (d, IH), 5.56 (s, IH), 4.50 (br. s, 2H), 3.65-3.56 (m, 2H), 2.93-2.87 (m, 2H), 2.09 (s, 3H).
MS (ESI+) mlz 413.3 [M + H]+.
Example 33
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-yl)-6-trifluoromethylpyrimi 2,4-diamine
Figure imgf000074_0002
1H NMR (300 MHz, DMSO- g ) ^ 11.01 (s, IH), 10.67 (s, IH), 9.14 (s, IH), 8.11 (m, IH), 7.76 (s, IH), 7.48 (s, IH), 7.33 (d, IH), 7.29 (t, IH), 7.20 (m, IH), 7.09 (m, 2H), 6.34 (br. s, IH), 6.26 (s, IH), 5.95 (s, IH), 4.64 (d, 2H), 2.32 (s, 3H).
MS (ESI+) mlz 437.3 [M + H]+. Example 34
N2,N4-Bis-(lH-indol-5-ylmethyl)-6-trifluoromethylpyrimidine-2^-diamine
Figure imgf000075_0001
1H NMR (300 MHz, CD3OD) δ 7.53 (m, IH), 7.50 (m, IH), 7.30 (m, 2H), 7.20 (m, 2H), 7.09 (m, 2H), 6.37 (d, 2H), 6.12 (s, IH), 4.66 (s, 4H).
MS (ESI+) mlz 437.3 [M + H]+.
Example 35
N2-(2-(lH-indol-3-yl)ethyl)-A/4-(lH-indol-5-ylmethyl)-6-trifluoromethylpy
2,4-diamine
Figure imgf000075_0002
1H NMR (300 MHz, CDC13) δ 8.20 (br. s, IH), 7.95 (br. s, IH), 7.65 (d, IH), 7.59 (s, IH), 7.35 (m, 2H), 7.26-7.09 (m, 4H), 7.00 (s, IH), 6.53 (s, IH), 6.02 (s, IH), 5.15 (br. s, 2H), 4.63 (br. s, 2H), 3.75 (q, 2H), 3.04 (t, 2H).
MS (ESI+) mlz 451.3 [M + H]+. Example 36
N2,N4-bis(lH-indol-5-ylmethyl)-6-benzylpyrimidine-2,4-diamine
Figure imgf000075_0003
1H NMR (500 MHz, DMSO- 6) δ 10.97 (s, IH), 10.94 (s, IH), 7.45 (s, IH), 7.41 (s, IH), 7.28-7.25 (m, 4H), 7.22 (m, 4H), 7.18 (m, 2H), 7.07 (dd, IH), 7.01 (dd, IH), 6.82 (br s, IH), 6.30 (m, 2H), 5.55 (s, IH), 4.49 (d, 4H), 3.58 (s, 2H). MS (ESr) mlz 459.3 [M + H]+. Example 37
N4-(lH-indazol-5-ylmethyl)-N2-(lH-indol-4-yl)-6-methylpyrimidine-2
Figure imgf000076_0001
1H-NMR (300 MHz, DMSO- 6): δ 12.98 (s, 1H), 10.95 (s, 1H), 8.34 (s, 1H), 7.99 (s, 1H), 7.79 (m, 1H), 7.63 (s, 1H), 7.57 (br s, 1H), 7.49 (d, 1H), 7.34 (d, 1H), 7.17 (t, 1H), 6.91 (m, 2H), 6.81 (br s, 1H), 5.86 (s, 1H), 4.61 (br s, 2H), 2.13 (s, 3H).
MS (ESI+) mlz 370.2 [M + H]+.
Example 38
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4,5-triamine
Figure imgf000076_0002
1H-NMR (500 MHz, DMSO-dg) ^ 11.00 (s, 1H), 10.89 (s, 1H), 7.87 (br. s, 1H), 7.82 (s, 1H), 7.54 (s, 1H), 7.44 (s, 1H), 7.34 (d, 1H), 7.29 (s, 1H), 7.15 (s, 2H), 6.88 (s, 2H), 6.80 (s, 2H), 6.36 (s, 1H), 4.74 (s, 2H), 4.14 (s, 2H).
MS (ESI+) mlz 370.2 [M + H]+.
Example 39
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)pyrimidine-2,4,5-triamine
Figure imgf000076_0003
1H-NMR (500 MHz, DMSO-dg) ^ 11.00 (s, IH), 10.72 (s, IH), 8.10 (s, IH), 7.98 (s, IH), 7.53 (s, IH), 7.40 (s, IH), 7.34 (d, IH), 7.29 (t, IH), 7.25 (dd, IH), 7.18 (t, IH), 7.16-7.13 (m, 2H), 6.72 (t, IH), 6.35 (br. s, IH), 6.22 (br. s, IH), 4.72 (d, 2H), 3.99 (s, 2H).
MS (ESI+) mlz 370.2 [M + H]+.
Example 40
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-6-yl)pyrimidine-2^,5-triamin
Figure imgf000077_0001
1H-NMR (300 MHz, CD3OD) δ 7.81-7.79 (m, IH), 7.60-7.59 (m, IH), 7.50 (s, IH), 7.45 (d, IH), 7.42 (d, IH), 7.23-7.18 (m, 2H), 7.07 (d, IH), 7.00 (dd, IH), 6.40 (dd, IH), 6.33 (dd, 1H), 4.81 (s, 2H).
MS (ESI+) mlz 370.3 [M + H]+. Example 41
N2,N4-bis(lH-indol-5-ylmethyl)pyrimidine-2,4,5-triamine
Figure imgf000077_0002
1H-NMR (500 MHz, CD3OD) δ 7.52 (s, IH), 7.48 (s, IH), 7.33 (s, IH), 7.26 (dd, 2H), 7.17 (dd, 2H), 7.09 (dd, IH), 7.07 (dd, IH), 6.34 (dd, 2H), 4.71 (s, 2H), 4.55 (s, 2H). MS (ESI+) mlz 384.3 [M + H]+.
Intermediate 42
N-(lH-indol-5-ylmethyl)-2-chloro-pyrimidin-4-amine
Figure imgf000078_0001
1H-NMR (500 MHz, CD3OD) δ 7.81 (s, IH), 7.51 (s, IH), 7.34 (d, IH), 7.21 (d, IH), 7.09 (d, IH), 6.40 (d, 2H), 4.61 (s, 2H).
MS (ESI+) mlz 259.1 [M + H]+.
Intermediate 43
N-(lH-indol-5-ylmethyl)-4-chloro-pyrimidin-2-amine
Figure imgf000078_0002
1H-NMR (500 MHz, CD3OD) δ 8.13 (d, IH), 7.51 (s, IH), 7.32 (d, IH), 7.19 (d, IH), 7.10 (dd, IH), 6.59 (d, IH), 6.38 (d, IH), 4.61 (s, 2H).
MS (ESI+) mlz 259.1 [M + H]+.
Intermediate 44
N-(2 -methyl- lH-indol-5-ylmethyl)-2-chloro-pyrimidin-4-amine
Figure imgf000078_0003
1H-NMR (500 MHz, DMSO-d6) δ 10.86 (s, IH), 8.27 (br s, IH), 7.90 (br s, IH), 7.33 (s, IH), 7.21 (d, IH), 6.95 (d, IH), 6.49 (d, IH), 6.07 (s, IH), 4.51 (br s, 2H), 2.36 (s, 3H).
MS (ESI+) mlz 273.1 [M + H]+.
Intermediate 45
N-(lH-indazol-5-ylmethyl)-2-chloro-pyrimidin-4-amine
Figure imgf000079_0001
1H-NMR (500 MHz, DMSO-d6) δ 13.03 (s, IH), 8.37 (br s, IH), 8.04 (br s, IH), 7.92 (m, IH), 7.67 (s, IH), 7.51 (d, IH), 7.31 (d, IH), 6.51 (d, 1H),4.58 (br s, 2H). Intermediate 46
N-(lH-benzorJ]imidazol-5-ylmethvn-2-chloro-pyrimidin-4-amine
Figure imgf000079_0002
1H NMR (500 MHz, DMSO-d6, 75°C) δ 12.22 (br s, IH), 8.21 (br s, IH), 8.13 (s, IH), 7.93 (d, IH), 7.53 (m, 2H), 7.17 (d, IH), 6.51 (d, IH), 4.59 (d, 2H).
MS (ESI+) m/z 260.2 [M + H]+.
Intermediate 47
N-(lH-indol-6-ylmethyl)-2-chloro-pyrimidin-4-amine
Figure imgf000079_0003
1H NMR (500 MHz, DMSO-d6, 75°C) (5 10.86 (br s, IH), 8.18 (br s, IH), 7.92 (d, IH), 7.49 (d, IH), 7.34 (s, IH), 7.27 (t, IH), 6.97 (d, IH), 6.50 (d, IH), 6.39 (br s, IH), 4.56 (d, 2H).
MS (ESI+) m/z 259.1 [M + H]+.
Intermediate 48
N-(lH-indol-6-ylmethyl)-4-chloro-pyrimidin-2-amine
Figure imgf000079_0004
1H NMR (500 MHz, DMSO-d6) δ 10.99 (br s, IH), 8.23-8.20 (m, 2H), 7.45 (d, IH), 7.30 (s, IH), 7.28 (t, IH), 6.95 (d, IH), 6.66 (d, IH), 6.36 (br s, IH), 4.57 (br s, 2H). MS (ESI+) mlz 259.0 [M + H]+.
Intermediate 49
N-(lH-indol-5-ylmethyl)-2-chloro-6-methyl-pyrimidin-4-amine
Figure imgf000080_0001
1H-NMR (300 MHz, CDC13) δ 8.23 (br. s, IH), 7.58 (s, IH), 7.40 (d, IH), 7.25 (s, IH), 7.13 (d, IH), 6.55-6.53 (m, IH), 6.11 (s, IH), 5.45 (br s, IH), 4.58 (br. s, 2H), 2.32 (s, 3H).
MS (ESI+) mlz 273.1 [M + H]+.
Intermediate 50
N-(lH-indol-5-ylmethyl)-4-chloro-6-methyl-pyrimidin-2-amine
Figure imgf000080_0002
1H-NMR (500 MHz, DMSO-dg) ^ 11.04 (s, IH), 8.16 (br s, IH), 7.46 (s, 1H),7.34 (d, IH), 7.32 (m, IH), 7.04 (d, IH), 6.38 (br s, IH), 6.32 (br s, IH), 4.53 (br s, 2H), 2.17 (s, 3H). Intermediate 51
N-(lH-indazol-5-ylmethyl)-2-chloro-6-methyl-pyrimidin-4-amine
Figure imgf000081_0001
Ή-NMR (500 MHz, CDC13) δ 10.37 (m, IH), 8.05 (d, IH), 7.71 (d, IH), 7.48 (m, IH), 7.38 (m, IH), 6.50 (s, IH), 5.62 (br s, IH), 4.73 (d, 2H), 2.32 (s, 3H). Intermediate 52
N-(lH-indol-5-ylmethyl)-2-chloro-6-trifluoromethyl-pyrimidin-4-amm
Figure imgf000081_0002
1H NMR (300 MHz, CDC13, 75°C) δ 8.16 (br s, IH), 7.60 (s, IH), 7.41 (d, IH), 7.24 (m, IH), 7.15 (d, IH), 6.58 (m, 2H), 5.62 (br s, IH), 4.69 (d, 2H).
MS (ESI+) mlz 327.1 [M + H]+.
Intermediate 53
N-(lH-indol-5-ylmethyl)-6-benzyl-2-chloro-pyrimidin-4-amine
Figure imgf000081_0003
1H NMR (500 MHz, DMSO-d6, 75°C) δ 10.86 (br s, IH), 8.04 (br s, IH), 7.45 (s,
IH), 7.34 (d, IH), 7.31-7.28 (m, 3H), 7.24-7.20 (m, 3H), 7.03 (d, IH), 6.38 (m, IH), 6.28 (s, IH), 4.50 (d, 2H), 3.80 (s, 2H).
MS (ESI+) mlz 349.2 [M + H]+. Intermediate 54 N-(lH-indol-5-ylmethyl)-2-chloro-5-nitro-pyrimidin-4-amine
Figure imgf000082_0001
1H-NMR (500 MHz, DMSO-d6) ^ 11.06 (s, IH), 9.53 (t, IH), 9.03 (s, IH) IH), 7.34 (d, IH), 7.32 (t, IH), 7.13 (dd, IH), 6.38 (br. s, IH), 4.80 (d, 2H).
MS (ESI+) mlz 304.1 [M + H]+.
Intermediate 55
N-(lH-indol-4-yl)-2-chloro-pyrimidin-4-amine
Figure imgf000082_0002
1H-NMR (500 MHz, CDC13) δ 8.39 (br s, IH), 8.07 (d, IH), 7.36 (d, IH), 7.26 (m, IH), 7.23 (t, IH), 7.18 (br s, IH), 7.10 (d, IH), 6.52 (d, IH), 6.44 (br s, IH).
MS (ESI+) mlz 245.1 [M + H]+.
Intermediate 56
N-[2-(lH-indol-3-yl)ethyll-2-chloro-pyrimidin-4-amine
Figure imgf000082_0003
1H NMR (500 MHz, DMSO-d6, 75°C) <5 10.66 (br s, IH), 7.90 (d, IH), 7.79 (br s, IH), 7.58 (d, IH), 7.35 (d, IH), 7.15 (s, IH), 7.07 (t, IH), 6.99 (t, IH), 6.44 (d, IH), 3.56 (m, 2H), 2.96 (t, 2H).
MS (ESI+) mlz 273.2 [M + H]+.
Intermediate 57
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)-5-nitropyrimidine-2,4-diamine
Figure imgf000083_0001
1H-NMR (500 MHz, DMSO-dg) δ 11.13 (s, IH), 10.99 (s, IH), 10.09 (s, IH), 9.20 (s, IH), 8.98 (s, IH), 7.44 (br. s, IH), 7.29-7.25 (m, 4H), 7.21 (d, IH), 7.04-6.99 (m, 2H), 6.68 (br. s, IH), 6.26 (br. s, IH), 4.70 (s, 2H).
MS (ESI+) mlz 400.3 [M + H]+.
Intermediate 58
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)-5-nitropyrimidine-2^-diami^
Figure imgf000083_0002
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.85 (s, 2H), 9.97 (br. s, IH), 9.05 (br. s, IH), 9.20 (s, IH), 7.91 (s, IH), 7.51 (s, IH), 7.33-7.30 (m, 3H), 7.28 (dt, 2H), 7.12 (d, IH), 6.33 (d, 2H), 4.85 (d, 2H).
MS (ESI+) mlz 400.2 [M + H]+. Intermediate 59
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-6-yl)-5-nitropyrimidine-2,4-diamine
Figure imgf000083_0003
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.86 (br. s, 2H), 10.07 (br. s, IH), 8.97 (br. s, IH), 8.95 (s, IH), 7.94 (s, IH), 7.54 (s, IH), 7.44 (d, IH), 7.35-7.31 (m, 2H), 7.28- 7.25 (m, 2H), 7.15 (d, IH), 6.38 (br. s, IH), 6.31 (br. s, IH), 4.88 (d, 2H).
MS (ESI+) mlz 400.3 [M + H]+.
Intermediate 60
Figure imgf000084_0001
1H-NMR (500 MHz, DMSO-d6, 105°C) δ 10.76 (m, 2H), 8.86 (br. s, 2H), 8.35 (br. s, IH), 7.52 (d, 2H), 7.32 (t, 2H), 7.26 (m, 2H), 7.10 (dd, 2H), 6.35 (s, 2H), 4.83 (d, 2H), 4.66 (d, 2H).
MS (ESI+) mlz 414.3 [M + H]+.
Intermediate 61
N-(2 -methyl- lH-indol-5-yl)-2-chloro-pyrimidin-4-amine
Figure imgf000084_0002
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.76 (s, IH), 9.54 (s, IH), 8.01 (d, IH), 7.47 (s, IH), 7.26 (d, IH), 7.01 (d, IH), 6.56 (d, IH), 6.11 (s, IH), 2.39 (s, 3H).
MS (ESI+) mlz 259.1 [M + H]+. Example 62
5-([2-(lH-indol-5-ylmethylamino)pyrimidin-4-ylaminolmethyl|indolin-2-one
Figure imgf000084_0003
1 H-NMR (500 MHz, CD3OD) δ 7.59 (d, IH), 7.43 (s, IH), 7.26 (d, IH), 7.17 (d, IH), 7.09 (d, IH), 7.07 (br s, IH), 7.03 (d, IH), 6.73 (d, IH), 6.32 (d, IH), 5.77 (d, IH), 4.58 (s, 2H), 4.48 (s, 2H), 3.31 (s, 2H).
MS (ESI+) mlz 385.3 [M + H]+.
Example 63 A/ 2-methynH-indol-5-yl)-N 2-methy
diamine
Figure imgf000085_0001
1H-NMR (500 MHz, DMSO-d6) δ 10.73 (d, 2H), 8.72 (s, IH), 7.74 (d, IH), 7.70 (s, IH), 7.32 (s, IH), 7.17-7.12 (m, 2H), 7.07 (d, IH), 6.99 (d, IH), 6.95 (br s, IH), 6.01 (m, 2H), 6.88 (d, IH), 5.51 (d, 2H), 2.34 (d, 6H).
MS (ESI+) mlz 383.3 [M + H]+.
Example 64
N2-r2-(5-methoxy- lH-indol-3-vnethyll-N4-(2-methyl- lH-indol-5- ylmethyl)pyrimidine-2 ,4-diamine
Figure imgf000085_0002
1H-NMR (500 MHz, DMSO- 6, 75°C) δ 10.60 (s, IH), 10.42 (s, IH), 7.66 (d, IH), 7.34 (s, IH), 7.22 (d, IH), 7.18 (d, IH), 7.07 (dd, 2H), 6.98 (m, 2H), 6.71 (dd, IH), 6.05 (br s, IH), 6.03 (s, IH), 5.77 (d, IH), 4.50 (d, 2H), 3.73 (s, 3H), 3.53 (q, 2H), 2.91 (t, 2H), 2.36 (s, 3H).
MS (ESI+) mlz 427.5 [M + H]+.
Example 65
N2-(lH-indazol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamine
Figure imgf000085_0003
1 H-NMR (500 MHz, DMSO-d6) δ 12.93 (s, IH), 10.74 (s, IH), 8.78 (s, IH), 7.95 (br s, IH), 7.75 (d, IH), 7.66 (br s, IH), 7.62 (s, IH), 7.45 (d, IH), 7.35 (d, IH), 7.13 (d, 2H), 7.05 (m, IH), 5.99 (br s, IH), 5.90 (d, IH), 4.55 (d, 2H), 2.35 (s, 3H).
MS (ESI+) m/z 370.3 [M + H]+.
Example 66
N4-(lH-indazol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyllpyrimidine-2^ diamine
Figure imgf000086_0001
1 H-NMR (500 MHz, DMSO-d6) δ 12.97 (s, IH), 10.60 (s, IH), 7.97 (br s, IH), 7.68- 7.62 (m, 2H), 7.46 (d, IH), 7.37 (br s, IH), 7.32 (d, IH), 7.20 (d, IH), 7.08 (s, IH), 7.04 (br s, IH), 6.69 (dd, IH), 6.41 (br s, IH), 5.76 (br s, IH), 4.56 (br s, 2H), 3.71 (s, 3H), 3.47 (q, 2H), 2.87 (t, 2H).
MS (ESI+) m/z 414.4 [M + H]+.
Example 67
N2-(lH-benzo[ imidazol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidm^ diamine
Figure imgf000086_0002
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 12.1 (br s, IH), 10.60 (s, IH), 8.78 (s, IH), 8.09 (s, IH), 7.76 (d, IH), 7.61 (s, IH), 7.55 (s, IH), 7.50 (d, IH), 7.21-7.15 (m, 2H), 7.08 (d, IH), 7.01 (br s, IH), 6.01 (s, IH), 5.96 (d, IH), 4.62 (d, 2H), 2.36 (s, 3H). MS (ESI+) mlz 370.4 [M + H]+. Example 68 A^-nH-benzor limidazol-S-ylmet^^
Figure imgf000087_0001
1H-NMR (500 MHz, DMSO-d6) δ 12.14 (br s, IH), 10.76 (s, IH), 8.10 (s, IH), 7.66 (d, IH), 7.60-7.40 (m, 3H), 7.26 (d, IH), 7.24 (t, IH), 7.15 (br s, 2H), 7.06 (d, IH), 6.47 (br s, IH), 6.32 (s, IH), 5.78 (d, IH), 4.58 (d, 2H), 4.51 (d, 2H).
MS (ESI+) mlz 370.3 [M + H]+.
Example 69
A^-(lH-benzor6nimidazol-5-ylmethyl -N2-r2-(5-methoxy-lH-indol-3- yl)ethyllpyrimidine-2,4-diamine
Figure imgf000087_0002
1H-NMR (500 MHz, CD3OD) δ 8.10 (s, IH), 7.60-7.52 (m, 3H), 7.27 (d, IH), 7.18 (d, IH), 6.97 (m, 2H), 6.71 (dd, IH), 5.81 (d, IH), 4.66 (br s, 2H), 3.72 (s, 3H), 3.60 (t, 2H), 2.93 (t, 2H).
MS (ESI+) mlz 414.3 [M + H]+.
Example 70
N2-(lH-indol-6-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2^-diamm
Figure imgf000087_0003
1H-NMR (500 MHz, CD3OD) δ 7.68 (d, IH), 7.49 (d, 2H), 7.36 (s, IH), 7.19 (s, IH), 7.16 (d, IH), 7.02 (dd, 2H), 6.38 (dd, IH), 6.03 (s, IH), 5.95 (d, IH), 4.64 (s, 2H), 2.38 (s, 3H).
MS (ESI+) mlz 369.4 [M + H]+. Example 71
A^-(lH-indol-6-ylmethyl -N2-r2-(5-methoxy-lH-indol-3-vnethyl1pyrimidine-2,4- diamine
Figure imgf000088_0001
1H-NMR (500 MHz, DMSO-d6) δ 10.98 (s, IH), 10.59 (s, IH), 7.66 (br s, IH), 7.44 (d, IH), 7.32 (br s, 2H), 7.27 (t, IH), 7.20 (d, IH), 7.07 (m, 2H), 6.96 (d, IH), 6.69 (dd, IH), 6.36 (m, 2H), 5.76 (br s, IH), 4.56 (br s, 2H), 3.71 (s, 3H), 3.48 (q, 2H), 2.87 (t, 2H).
MS (ESI+) mlz 413.3 [M + H]+.
Example 72
A^-(lH-indol-5-ylmethyl -N2-(2 5-(benzyloxy -lH-indol-3-yl1ethyl|pyrimidine-2,4- diamine
Figure imgf000088_0002
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.80 (s, IH), 10.45 (s, IH), 7.66 (d, IH), 7.48 (s, IH), 7.44 (m, 2H), 7.36 (t, 2H), 7.32-7.29 (m, 2H), 7.25 (t, IH), 7.23 (d, IH), 7.18 (d, IH), 7.09-7.06 (m, 2H), 7.03 (br s, IH), 6.80 (dd, IH), 6.34 (br s, IH), 6.06 (br s, IH), 5.78 (d, IH), 5.06 (s, 2H), 4.53 (d, 2H), 3.53 (q, 2H), 2.90 (t, 2H).
MS (ESI+) mlz 489.4 [M + H]+. Example 73
Figure imgf000089_0001
yl] ethyl) pyrimidine-2,4-diamine
Figure imgf000089_0002
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.80 (s, IH), 10.42 (s, IH), 7.66 (d, IH), 7.49 (s, IH), 7.32 (d, IH), 7.26 (t, IH), 7.21 (d, IH), 7.10-7.07 (m, 3H), 7.02 (m, IH), 6.72 (dd, IH), 6.35 (br s, IH), 6.05 (t, IH), 5.78 (d, IH), 4.54 (d, 2H), 4.06 (t, 2H), 3.58 (m, 4H), 3.53 (q, 2H), 2.91 (t, 2H), 2.67 (t, 2H), 2.51-2.46 (m, 4H).
MS (ESI+) mlz 512.4 [M + H]+.
Example 74
Figure imgf000089_0003
yl] ethyl) pyrimidine-2,4-diamine
Figure imgf000089_0004
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.81 (s, IH), 10.43 (s, IH), 7.66 (d, IH), 7.49 (s, IH), 7.32 (d, IH), 7.26 (t, IH), 7.22 (d, IH), 7.08 (m, 3H), 7.01 (m, IH), 6.72 (dd, IH), 6.35 (br s, IH), 6.06 (m, IH), 5.78 (d, IH), 4.54 (d, 2H), 4.06 (t, 2H), 3.63 (t, 2H), 3.53 (q, 2H), 3.32 (s, 3H), 2.90 (t, 2H).
MS (ESI+) mlz 457.5 [M + H]+.
Example 75
A^-dH-indol-S-ylmethyD-A^-d -methyl- lH-indol-4-yl)pyrimidine-2,4-diamine
Figure imgf000090_0001
1H-NMR (500 MHz, DMSO-dg) ^ 11.00 (s, IH), 8.37 (s, IH), 7.91 (d, IH), 7.81 (d, IH), 7.59 (br s, IH), 7.50 (s, IH), 7.33 (d, IH), 7.30 (t, IH), 7.17 (d, IH), 7.09 (dd, IH), 7.02-6.98 (m, 2H), 6.80 (d, IH), 6.36 (br s, IH), 5.99 (d, IH), 4.61 (br s, 2H), 3.73 (s, 3H).
MS (ESI+) mlz 369.4 [M + H]+.
Example 76
N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-4-yl)pyrimidine-2^-diamine
Figure imgf000090_0002
1H-NMR (500 MHz, DMSO- 6, 75°C) δ 12.68 (s, IH), 10.83 (s, IH), 8.74 (s, IH), 8.40 (s, IH), 7.89 (d, IH), 7.86 (d, IH), 7.51 (s, IH), 7.42 (m, IH), 7.35 (d, IH), 7.27 (m, IH), 7.18 (t, IH), 7.10 (d, IH), 7.05 (d, IH), 6.37 (m, IH), 6.07 (d, IH), 4.62 (d, 2H).
MS (ESI+) mlz 356.3 [M + H]+.
Example 77
A/ lH-indol-5-ylmethyl)-N n-mem^
diamine
Figure imgf000090_0003
1H-NMR (500 MHz, DMSO- 6) δ 10.99 (s, IH), 7.61 (d, IH), 7.45 (m, 2H), 7.29- 7.24 (m, 5H), 7.12 (dd, IH), 7.03 (d, IH), 6.83 (br s, IH), 6.33 (br s, IH), 6.31 (m, IH), 5.73 (d, IH), 4.50 (m, 4H), 3.74 (s, 3H). MS (ESr) mlz 383.4 [M + H]+.
Example 78
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-ylmethyl)pyrimidm
Figure imgf000091_0001
1H-NMR (500 MHz, DMSO-d6) δ 10.99 (d, 2H), 7.61 (m, IH), 7.43 (br s, IH), 7.29- 7.23 (m, 5H), 7.02 (d, IH), 6.96 (t, IH), 6.92 (d, IH), 6.78 (br s, IH), 6.58 (s, IH), 6.34 (s, IH), 5.74 (m, IH), 4.70 (d, 2H), 4.49 (br s, 2H).
MS (ESI+) mlz 369.3 [M + H]+.
Example 79
N4-(lH-indol-5-ylmethyl)-N2-[(9H-carbazol-3-yl)methyllpyrimidine-2,4-diamine
Figure imgf000091_0002
1H-NMR (500 MHz, DMSO-dg) δ 11.15 (s, IH), 11.0 (s, IH), 8.03 (s, IH), 7.98 (br s, IH), 7.63 (br d, IH), 7.45 (m, 2H), 7.38-7.27 (m, 6H), 7.10 (t, IH), 7.05 (d, IH), 6.92 (br s, IH), 6.32 (br s, IH), 5.75 (br d, IH), 4.59 (d, 2H), 4.54 (br s, 2H).
MS (ESI+) mlz 419.3 [M + H]+.
Example 80
N2-(lH-indol-5-ylmethyl)-N4-[(9H-carbazol-3-yl)methyllpyrimidine-2,4-diamine
Figure imgf000091_0003
1H-NMR (500 MHz, DMSO-dg) ^ 11.17 (s, IH), 10.94 (s, IH), 8.04 (s, IH), 8.01 (br s, IH), 7.63 (d, IH), 7.46-7.44 (m, 2H), 7.41-7.32 (m, 4H), 7.28-7.25 (m, 2H), 7.11 (t, IH), 7.08 (d, IH), 6.84 (br s, IH), 6.31 (s, IH), 5.75 (d, IH), 4.60 (br s, 2H), 4.51 (d, 2H).
MS (ESI+) mlz 419.3 [M + H]+. Example 81
Methyl 4-[4-(lH-indol-5-ylmethylamino)pyrimidin-2-ylaminol-lH-indole-6- carboxylate
Figure imgf000092_0001
1H-NMR (500 MHz, DMSO-dg) (5 11.38 (s, IH), 11.00 (s, IH), 8.85 (br s, IH), 8.66 (s, IH), 7.84 (d, IH), 7.70 (s, IH), 7.57 (br s, IH), 7.52 (s, IH), 7.45 (t, IH), 7.32 (d, IH), 7.29 (t, IH), 7.11 (dd, IH), 6.99 (br s, IH), 6.34 (br s, IH), 6.02 (d, IH), 4.73 (br s, 2H), 3.70 (s, 3H).
MS (ESI+) mlz 413.3 [M + H]+.
Example 82
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-5-yl)pyrimidine-2,4-diamine
Figure imgf000092_0002
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.76 (s, 2H), 8.59 (s, IH), 7.81 (s, IH), 7.77 (d, IH), 7.50 (s, IH), 7.30 (m, 2H), 7.25 (m, 2H), 7.18 (dd, IH), 7.11 (d, IH), 6.67 (br s, IH), 6.33 (br s, 2H), 5.93 (d, IH), 4.57 (d, 2H).
MS (ESI+) mlz 355.2 [M + H]+. Example 83
N2-(lH-indol-5-ylmethyl)-6-methyl-N4-(2-methyl-lH-indol-5-yl)py
diamine
Figure imgf000093_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.77 (s, IH), 10.55 (s, IH), 8.37 (s, IH), 7.60 (s, IH), 7.50 (s, IH), 7.31 (d, IH), 7.25 (t, IH), 7.16-7.10 (m, 2H), 7.06 (d, IH), 6.53 (br s, IH), 6.34 (s, IH), 6.02 (s, IH), 5.78 (s, IH), 4.57 (d, 2H), 2.36 (s, 3H), 2.07 (s, 3H).
MS (ESI+) mlz 383.4 [M + H]+.
Example 84
Λ^-(1^ίηάο1-5-ν^6ί1ιν1 -Λ^-Γ2-(5^6ί1ιοχν-1^ίηάο1-3-νη6ί1ιν11-6- methylpyrimidine-2,4-diamine
Figure imgf000093_0002
1H-NMR (500 MHz, DMSO-dg) δ 10.98 (s, IH), 10.60 (s, IH), 7.46 (s, IH), 7.31- 7.27 (m, 2H), 7.21 (d, IH), 7.14-7.03 (m, 4H), 6.69 (dd, IH), 6.33 (s, IH), 6.29 (br s, IH), 5.62 (s, IH), 4.53 (br s, 2H), 3.70 (s, 3H), 3.48 (q, 2H), 2.88 (t, 2H), 2.01 (s, 3H).
MS (ESI+) mlz 427.4 [M + H]+.
Example 85
N4-(lH-indol-5-ylmethyl -6-benzyl-N2-r2-(5-methoxy-lH-indol-3- yl)ethyllpyrimidine-2,4-diamine
Figure imgf000094_0001
1 H-NMR (500 MHz, DMSO-d6) δ 10.79 (s, 1H), 10.42 (s, 1H), 7.45 (s, 1H), 7.30- 7.16 (m, 8H), 7.07- 7.03 (m, 3H), 6.94 (m, 1H), 6.71 (dd, 1H), 6.33 (s, 1H), 6.07 (br s, 1H), 5.62 (s, 1H), 4.51 (d, 2H), 3.71 (s, 3H), 3.63 (s, 2H), 3.54 (q, 2H), 2.91 (t, 2H). MS (ESI+) mlz 503.4 [M + H]+.
Example 86
N*-( lH-indol-5 - ylmethvO-N2- Γ2-Γ5 -methoxy-7-methyl- 1 H-indol-3 - yl)ethyllpyrimidine-2,4-diamine
Figure imgf000094_0002
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.81 (s, 1H), 10.38 (s, 1H), 7.66 (d, 1H), 7.48 (s, 1H), 7.32 (d, 1H), 7.26 (t, 1H), 7.08-7.06 (m, 2H), 7.02 (m, 1H), 6.89 (s, 1 H), 6.53 (s, 1H), 6.35 (s, 1H), 6.05 (m, 1H), 5.77 (d, 1H), 4.54 (d, 2H), 3.71 (s, 3H), 3.53 (q, 2H), 2.90 (t, 2H), 2.40 (s, 3H).
MS (ESI+) mlz 427.4 [M + H]+.
Example 87
Λ^-(1^ίηάο1-5-ν^6ί1ινη-Λ^-Γ2-(5-6ί1ιοχν-1^ίηάο1-3-νΠ6ί1ιν11ρνΓ ίάίη6-2,4- diamine
Figure imgf000094_0003
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.81 (s, IH), 10.41 (s, IH), 7.66 (d, IH), 7.49 (s, IH), 7.32 (d, IH), 7.26 (t, IH), 7.21 (d, IH), 7.09-7.06 (m, 3H), 7.01 (m, IH), 6.70 (dd, IH), 6.35 (m, IH), 6.06 (m, IH), 5.78 (d, IH), 4.54 (d, 2H), 3.99 (q, 2H), 3.53 (q, 2H), 2.90 (t, 2H), 1.30 (t, 3H).
MS (ESI+) mlz 427.3 [M + H]+.
Example 88
N*-( lH-indol-5 - ylmethvO-N2- (2- Γ5 -(trifluoromethoxy - lH-indol-3 - yl] ethyl) pyrimidine-2,4-diamine
Figure imgf000095_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.90 (s, IH), 10.80 (s, IH), 7.66 (d, IH), 7.52 (s, IH), 7.48 (s, IH), 7.41 (d, IH), 7.31 (d, IH), 7.26 (m, 2H), 7.07 (d, IH), 7.01- 7.00 (m, 2H), 6.34 (s, IH), 6.12 (t, IH), 5.78 (d, IH), 4.53 (d, 2H), 3.53 (q, 2H), 2.93 (t, 2H).
MS (ESI+) mlz 467.2 [M + H]+. Example 89
A^-(lH-indol-5-ylmethvn-N2 2-(5-fluoro-lH-indol-3-vnethyl1pyrimidine-2,4- diamine
Figure imgf000095_0002
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.81 (s, IH), 10.71 (s, IH), 7.66 (d, IH), 7.48 (s, IH), 7.33-7.28 (m, 3H), 7.26 (t, IH), 7.20 (s, IH), 7.07 (d, IH), 7.03 (br s, IH), 6.87 (m, IH), 6.35 (s, IH), 6.09 (br s, IH), 5.78 (d, IH), 4.54 (d, 2H), 3.52 (q, 2H), 2.90 (t, 2H). MS (ESr ) mlz 401.3 [M + H]+. Example 90
A^-(lH-indol-5-ylmethvn-N2 2-(6-methoxy-lH-indol-3-vnethyl1pyrimidine-2,4- diamine
Figure imgf000096_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.81 (s, IH), 10.37 (s, IH), 7.66 (d, IH), 7.49 (s, IH), 7.41 (d, IH), 7.32 (d, IH), 7.26 (t, IH), 7.08 (d, IH), 7.03 (m, IH), 6.97 (s, IH), 6.85 (d, IH), 6.60 (dd, IH), 6.35 (s, IH), 6.05 (m, IH), 5.78 (d, IH), 4.55 (d, 2H), 3.75 (s, 3H), 3.53 (q, 2H), 2.89 (t, 2H).
MS (ESI+) mlz 413.3 [M + H]+.
Example 91
A^-(lH-indol-5-ylmethvn-N2-r2-(7-methoxy-lH-indol-3-vnethyl1pyrimidine-2,4- diamine
Figure imgf000096_0002
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.81 (s, IH), 10.62 (s, IH), 7.66 (d, IH), 7.49 (s, IH), 7.32 (d, IH), 7.26 (t, IH), 7.16 (d, IH), 7.08 (d, IH), 7.04-7.00 (m, 2H), 6.87 (t, IH), 6.63 (d, IH), 6.36 (s, IH), 6.06 (br s, IH), 5.77 (d, IH), 4.54 (d, 2H), 3.90 (s, 3H), 3.53 (q, 2H), 2.92 (t, 2H).
MS (ESI+) mlz 413.3 [M + H]+.
Example 92
N2-(lH-indol-5-ylmethyl)-N4-(l,2-dimethyl-lH-indol-5-yl)pyrimidine-2^-diam
Figure imgf000097_0001
Ή-NMR (500 MHz, DMSO-d6, 75°C) δ 10.77 (s, IH), 8.57 (s, IH), 7.77 (d, IH), 7.69 (d, IH), 7.50 (d, IH), 7.31 (d, IH), 7.24 (dd, IH), 7.23 (d, IH), 7.16 (dd, IH), 7.11 (d, IH), 6.65 (br s, IH), 6.34 (s, IH), 6.10 (s, IH), 5.91 (s, IH), 4.57 (d, 2H), 3.63 (s, 3H), 2.38 (s, 3H).
MS (ESI+) mlz 383.2 [M + H]+.
Example 93
methyl 5-[2-(lH-indol-5-ylmethylamino)pyrimidin-4-ylaminol-lH-indole-2- carboxylate
Figure imgf000097_0002
1H-NMR (500 MHz, CD3OD) δ 7.87 (s, IH), 7.74 (d, IH), 7.52 (s, IH), 7.36-7.30 (m, 3H), 7.18 (d, IH), 7.13 (dd, IH), 7.06 (s, IH), 6.38 (d, IH), 5.98 (d, IH), 4.62 (s, 2H), 3.90 (s, 3H).
MS (ESI+) mlz 413.3 [M + H]+.
Example 94
N2-(lH-indol-5-ylmethyl)-N4-(2J-dimethyl-lH-indol-5-yl)pyrimidine-2^-diamm^
Figure imgf000097_0003
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.77 (br s, IH), 10.31 (s, IH), 8.55 (s, IH), 7.76 (d, IH), 7.64 (s, IH), 7.48 (s, IH), 7.29 (d, IH), 7.24 (t, IH), 7.13-7.07 (m, 3H), 6.57 (br s, IH), 6.33 (s, IH), 5.91 (d, IH), 4.60 (d, 2H), 2.28 (s, 3H), 2.07 (s, 3H). MS (ESr ) mlz 383.3 [M + H]+. Example 95
Figure imgf000098_0001
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 12.07 (br s, 1H), 10.77 (br s, 1H), 8.83 (s, 1H), 8.05 (s, 1H), 7.98 (s, 1H), 7.82 (d, 1H), 7.51 (s, 1H), 7.45 (m, 1H), 7.34 (d, 1H), 7.30 (d, 1H), 7.24 (t, 1H), 7.12 (d, 1H), 6.71 (br s, 1H), 6.32 (s, 1H), 5.99 (d, 1H), 4.59 (d, 2H).
MS (ESI+) mlz 356.1 [M + H]+.
Example 96
N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-benzo[ imidazol-5-yl)pyrimidm^ diamine
Figure imgf000098_0002
1 H-NMR (500 MHz, DMSO-d6, 75°C) ^ 11.78 (br s, 1H), 10.77 (s, 1H), 8.76 (br s, 1H), 7.82-7.79 (m, 2H), 7.51 (s, 1H), 7.34-7.23 (m, 4H), 7.12 (dd, 1H), 6.66 (br s, 1H), 6.33 (s, 1H), 5.97 (d, 1H), 4.58 (d, 2H), 2.44 (s, 3H).
MS (ESI+) mlz 370.2 [M + H]+.
Example 97
N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-4-yl)-6-methylpyrimidine-2,4-diamine
Figure imgf000099_0001
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 12.66 (s, 1H), 10.82 (s, 1H), 8.70 (br s, 1H), 8.43 (s, 1H), 7.94 (d, 1H), 7.50 (s, 1H), 7.34 (d, 1H), 7.29 (br s, 1H), 7.27 (dd, 1H), 7.16 (dd, 1H), 7.09 (d, 1H), 7.02 (d, 1H), 6.36 (s, 1H), 5.94 (s, 1H), 4.61 (d, 2H), 2.16 (s, 3H).
MS (ESI+) mlz 370.2 [M + H]+.
Example 98
N2 2-(5-methoxy-lH-indol-3-vnethyl1-6-methyl-A^-r(2-methyl-lH-indol-5^ yl)methyllpyrimidine-2,4-diamine
Figure imgf000099_0002
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.59 (s, 1H), 10.43 (s, 1H), 7.33 (s, 1H), 7.22 (d, 1H), 7.17 (d, 1H), 7.08 (d, 1H), 7.06 (s, 1H), 6.97 (d, 1H), 6.85 (br s, 1H), 6.71 (dd, 1H), 6.02 (s, 1H), 5.99 (br s, 1H), 5.65 (s, 1H), 4.50 (d, 2H), 3.73 (s, 3H), 3.53 (dd, 2H), 2.91 (t, 2H), 2.36 (s, 3H), 2.03 (s, 3H).
MS (ESI+) mlz 441.4 [M + H]+.
Example 99
N4-(lH-indazol-5-ylmethvn-N2-r2-(5-methoxy-lH-indol-3-vnethyl1-6- methylpyrimidine-2,4-diamine
Figure imgf000099_0003
Ή-NMR (500 MHz, DMSO-d6, 75°C) δ 12.79 (s, IH), 10.43 (s, IH), 7.94 (s, IH), 7.65 (s, IH), 7.45 (d, IH), 7.33 (d, IH), 7.21 (d, IH), 7.07 (d, IH), 7.04 (s, IH), 7.02 (br s, IH), 6.71 (d, IH), 6.03 (br s, IH), 5.66 (s, IH), 4.57 (d, 2H), 3.74 (s, 3H), 3.52 (dd, 2H), 2.89 (t, 2H), 2.04 (s, 3H).
MS (ESI+) mlz 428.4 [M + H]+.
Example 100
N*-( lH-indol-5 - ylmethylVN2- Γ2-Γ5 -methoxy-2-methyl- 1 H-indol-3 - vDethyll -6- methylpyrimidine-2,4-diamine
Figure imgf000100_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.84 (s, IH), 10.33 (s, IH), 7.56 (br s, IH), 7.48 (s, IH), 7.33 (d, IH), 7.27 (dd, IH), 7.11 (d, IH), 7.06 (d, IH), 6.97 (d, IH), 6.62 (dd, IH), 6.42 (br s, IH), 6.36 (s, IH), 5.76 (s, IH), 4.58 (d, 2H), 3.70 (s, 3H), 3.47 (dd, 2H), 2.87 (t, 2H), 2.29 (s, 3H), 2.09 (s, 3H).
MS (ESI+) mlz 441.3 [M + H]+.
Example 101
A^-(lH-indol-5-ylmethyl -N2-r2-(4-methoxy-lH-indol-3-yl ethyl1pyrimidine-2,4- diamine
Figure imgf000100_0002
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.80 (s, IH), 10.56 (s, IH), 7.63 (d, IH), 7.48 (s, IH), 7.31 (d, IH), 7.26 (dd, IH), 7.07 (dd, IH), 7.05-6.91 (m, 4H), 6.43 (dd, IH), 6.34 (s, IH), 5.94 (m, IH), 5.75 (d, IH), 4.51 (d, 2H), 3.85 (s, 3H), 3.54 (dd, 2H), 3.06 (t, 2H). MS (ESI+) mlz 413.3 [M + H]4
Example 102
4-[4-(lH-indol-5-ylmethylamino)pyrimidin-2-ylaminol-lH-indole-6-carboxylic acid
Figure imgf000101_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 12.12 (br s, 1H), 11.42 (s, 1H), 10.87 (s, 1H), 9.70 (br s, 1H), 8.69 (br s, 1H), 8.38 (s, 1H), 7.91 (s, 1H), 7.82 (d, 1H), 7.52 (s, 1H), 7.47 (s, 1H), 7.32 (d, 1H), 7.28 (dd, 1H), 7.06 (d, 1H), 6.77 (s, 1H), 6.34 (s, 1H), 6.22 (d, 1H), 4.69 (d, 2H).
MS (ESI+) mlz 399.3 [M + H]+.
Example 103
N2-(lH-indol-4-yl)-6-methyl-N4-[(2-methyl H-indol-5-yl)methyllpyrimidine-2,4- diamine
Figure imgf000101_0002
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.78 (s, 1H), 10.61 (s, 1H), 7.93 (s, 1H), 7.89 (d, 1H), 7.35 (s, 1H), 7.19 (d, 1H), 7.17 (dd, 1H), 7.00-6.94 (m, 3H), 6.74 (s, 1H), 6.16 (s, 1H), 6.05 (s, 1H), 5.87 (s, 1H), 4.56 (d, 2H), 2.36 (s, 3H), 2.14 (s, 3H). MS (ESI+) mlz 383.3 [M + H]+.
Example 104
(5-[2-(lH-indol-5-ylmethylamino)pyrimidin-4-ylaminol-lH-indol-2-yl|methanol
Figure imgf000102_0001
1 H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.77 (s, IH), 10.64 (s, IH), 8,55 (s, IH), 7.76 (d, 1 H), 7.72 (d, IH), 7.50 (s, IH), 7.31 (d, IH), 7.26-7.22 (m, 2H), 7.15-7.10 (m, 2H), 6.64 (br m, IH), 6.35 (s, IH), 6.19 (s, IH), 5.91 (d, IH), 4.95 (t, IH), 4.61- 4.56 (m, 4H).
MS (ESI+) mlz 385.3 [M + H]+. Example 105
N2-(lH-indol-5-ylmethvn-A^-methyl-A^-(2-methyl-lH-indol-5-vnpyrimidine-2,4- diamine
Figure imgf000102_0002
1 H-NMR (500 MHz, DMSO-d6) δ 11.03 (s, IH), 10.96 (s, IH), 7.57 (d, IH), 7.48 (s, IH), 7.33-7.27 (m, 3H), 7.25 (s, IH), 7.10 (d, IH), 6.99 (br s, IH), 6.83 (d, IH), 6.36 (s, IH), 6.12 (s, IH), 5.35 (d, IH), 4.52 (d, 2H), 3.38 (s, 3H), 2.38 (s, 3H).
MS (ESI+) mlz 383.2 [M + H]+.
Example 106
N2-qH-indol-5-ylmethylVA/ l.2-dm^
diamine
Figure imgf000102_0003
Ή-NMR (500 MHz, CD3OD) δ 7.54 (s, IH), 7.49 (d, IH), 7.35 (d, IH), 7.34 (d, IH), 7.28 (d, IH), 7.20 (d, IH), 7.14 (d, IH), 6.92 (dd, IH), 6.40 (d, IH), 6.22 (s, IH), 5.49 (d, IH), 4.63 (s, 2H), 3.70 (s, 3H), 3.45 (s, 3H), 2.43 (s, 3H).
MS (ESI+) mlz 397.3 [M + H]+.
Example 107
N*-( lH-indol-5 - ylmethvO-N2- Γ2-Γ5 -methoxy- 1 -methyl- 1 H-indol-3 - yl)ethyllpyrimidine-2,4-diamine
Figure imgf000103_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.83 (s, IH), 7.66 (d, IH), 7.49 (s, IH), 7.42 (br s, IH), 7.33 (d, IH), 7.27 (dd, IH), 7.24 (d, IH), 7.09-7.05 (m, 2H), 7.02 (s, IH), 6.78 (dd, IH), 6.42 (br s, IH), 6.35 (s, IH), 5.85 (d, IH), 4.56 (d, 2H), 3.74 (s, 3H), 3.67 (s, 3H), 3.54 (dd, 2H), 2.91 (t, 2H).
MS (ESI+) mlz 427.4 [M + H]+.
Example 108
N4-(lH-indol-5-ylmethyl -N2-r2-(5-methoxy-l-methyl-lH-indol-3-yl ethyl1-N2- methylpyrimidine-2,4-diamine
Figure imgf000103_0002
1H-NMR (500 MHz, CDC13) δ 8.21 (br s, IH), 7.93 (d, IH), 7.60 (s, IH), 7.34 (d, IH), 7.21 (dd, IH), 7.19-7.12 (m, 3H), 6.85 (dd, IH), 6.78 (s, IH), 6.51 (s, IH), 5.69 (d, IH), 4.89 (br s, IH), 4.61 (s, 2H), 3.86 (dd, 2H), 3.00 (t, 2H), 3.78 (s, 3H), 3.66 (s, 3H), 3.11 (s, 3H).
MS (ESI+) mlz 441.40 [M + H]+. Example 109
^-(lH-indol-S-ylmethvn-A^-r -fS-methoxy-lH-indol-S-vnethyll-N2- methylpyrimidine-2,4-diamine
Figure imgf000104_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.80 (s, 1H), 10.42 (s, 1H), 7.74 (d, 1H), 7.49 (s, 1H), 7.31 (d, 1H), 7.26 (dd, 1H), 7.21 (d, 1H), 7.15-7.04 (m, 4H), 6.71 (dd, 1H), 6.34 (s, 1H), 5.80 (d, 1H),4.56 (d, 2H), 3.81 (t, 2H), 3.73 (s, 3H), 3.05 (s, 3H), 2.93 (t, 2H).
MS (ESI+) mlz 427.3 [M + H]+.
Intermediate 110
N-(2-chloropyrimidin-4-yl)- 1 ,2-dimethyl- lH-indol-5-amine
Figure imgf000104_0002
1H-NMR (500 MHz, DMSO-d6) δ 9.57 (s, 1H), 8.02 (d, 1H), 7.51 (s, 1H), 7.35 (d, 1H), 7.10 (d, 1H), 6.58 (d, 1H), 6.20 (s, 1H), 3.66 (s, 3H), 2.41 (s, 3H).
MS (ESI+) mlz 273.0 [M + H]+.
Intermediate 111
methyl 5-(2-chloropyrimidin-4-ylamino)- lH-indole-2-carboxylate
Figure imgf000104_0003
1H-NMR (500 MHz, DMSO-d6, 75°C) ^ 11.76 (br s, 1H), 9.70 (s, 1H), 8.07 (d, 1H), 7.80 (s, 1H), 7.47 (d, 1H), 7.33 (dd, 1H), 7.14 (s, 1H), 6.65 (d, 1H), 3.89 (s, 3H). MS (ES ) mlz 303.1 [M + H]4
Intemediate 112
N-(2-chloropyrimidin-4-yl)-2,3-dimethyl-lH-indol-5-amine
Figure imgf000105_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.52 (s, IH), 9.55 (s, IH), 8.01 (d, IH), 7.42 (s, IH), 7.23 (d, IH), 7.02 (d, IH), 6.56 (d, IH), 2.32 (s, 3H), 2.14 (s, 3H).
MS (ESI+) mlz 273.2 [M + H]+. Intermediate 113
N-(2-chloropyrimidin-4-yl)-lH-benzo[(i imidazol-5-amine
Figure imgf000105_0002
1H-NMR (500 MHz, DMSO-d6) δ 12.38 (br s, IH), 10.00 (br s, IH), 8.19 (s, IH), 8.10 (d, IH), 7.94 (br s, IH), 7.58 (d, IH), 7.22 (d, IH), 6.71 (d, IH).
MS (ESI+) mlz 246.1 [M + H]+.
N-(2-chloropyrimidin-4-yl)-2-methyl-lH-benzor 1imidazol-5-amine
Figure imgf000105_0003
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 12.01 (s, IH), 9.75-9.68 (m, IH), 8.07 (s, IH), 7.74-7.69 (m, IH), 7.46-7.36 (m, IH), 7.18-7.12 (m, IH), 6.68-6.64 (m, IH), 2.48 (s, 3H).
MS (ESI+) mlz 260.1 [M + H]+. Intermediate 115
2-chloro-6-methyl-N-[(2-methyl-lH-indol-5-yl)methyllpyrimidin-4-amine
Figure imgf000106_0001
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.66 (br s, IH), 7.93 (br s, IH), 7.33 (s, IH), 7.21 (d, IH), 6.95 (d, IH), 6.33 (s, IH), 6.07 (s, IH), 4.49 (d, 2H), 2.37 (s, 3H), 2.18 (s, 3H).
MS (ESI+) mlz 287.1 [M + H]+.
Intermediate 116
2-[(tert-butyldimethylsilyloxy)mem^
Figure imgf000106_0002
1H-NMR (500 MHz, DMSO-d6, 75°C) δ 10.86 (s, IH), 9.57 (s, IH), 8.02 (d, IH), 7.57 (s, IH), 7.36 (d, IH), 7.09 (dd, IH), 6.59 (d, IH), 6.31 (s, IH), 4.80 (s, 2H), 0.92 (s, 9H), 0.10 (s, 6H).
MS (ESI+) mlz 389.2 [M + H]+.
Intermediate 117
N-(2-chloropyrimidin-4-yl)-N,2-dimethyl-lH-indol-5-amine
Figure imgf000106_0003
1H-NMR (500 MHz, DMSO-d6) δ 1 1.14 (s, IH), 7.86 (d, IH), 7.37 (d, IH), 7.34 (s, IH), 6.89 (dd, IH), 6.16 (s, IH), 6.07 (br s, IH), 3.39 (s, 3H), 2.39 (s, 3H).
MS (ESI+) mlz 273.2 [M + H]+. Intermediate 118
N-(2-chloropyrimidin-4-yl -N, 1 ,2-trimethyl- lH-indol-5-amine
Figure imgf000107_0001
1 H-NMR (500 MHz, DMSO-d6) δ 7.86 (d, IH), 7.50 (d, IH), 7.38 (s, IH), 6.98 (dd, IH), 6.26 (s, IH), 6.06 (br s, IH), 3.69 (s, 3H), 3.40 (s, 3H), 2.40 (s, 3H).
MS (ESI+) mlz 287.1 [M + H]+.
Intermediate 1 19
4-chloro-N-[2-(5-methoxy- 1 -methyl- lH-indol-3-yl)ethyl1pyrimidin-2-amine
Figure imgf000107_0002
1 H-NMR (500 MHz, DMSO-< , 75°C) δ 8.23 (d, IH), 7.46 (m, IH), 7.25 (d, IH), 7.08 (m, 2H), 6.79 (dd, IH), 6.63 (d, IH), 3.78 (s, 3H), 3.69 (s, 3H), 3.54 (q, 2H), 2.91 (t, 2H).
MS (ESI+) mlz 317.3 [M + H]+. Intermediate 120
4-chloro-N-[2-(5-methoxy- 1 -methyl- lH-indol-3-yl)ethyl1-N-methylpyrimidin-2- amine
Figure imgf000107_0003
1 H-NMR (500 MHz, CDC13) δ 8.17 (d, 1H), 7.18-7.16 (m, 2H), 6.88 (dd, 1H), 6.85 (s, 1H), 6.48 (s, 1H), 3.88-3.85 (m, 5H), 3.71 (s, 3H), 3.13 (s, 3H), 3.00 (m, 2H). MS (ESI+) mlz 331.2 [M + H]+.
Biological Assays
The Fluorometric Microculture Cytotoxicity Assay, FMCA, is a three day non- clonogenic microplate-based cell viability assay used for measurement of the cytotoxic and/or cytostatic effect of compounds in vitro (Lindhagen, E., et al. Nat Protoc, 2008. 3(8): p. 1364-9). FMCA (Larsson, R. and P. Nygren, Anticancer Res, 1989. 9(4): p. 1111-9) represents a valuable method to measure cytotoxicity in a number of cell types, both cell lines and primary cells from patients (Larsson, R., et al, Int J Cancer, 1992. 50(2): p. 177-85; Fridborg, H., et al, Eur J Cancer, 1999. 35(3): p. 424-32; Dhar, S., et al, Br J Cancer, 1996. 74(6): p. 888-96). FMCA is based on the principle that fluorescein diacetate (FDA) is converted to the fluorescent probe fluorescein by esterases in the plasma membranes of living cells. For experiments, 96 or 384-well microplates are prepared with compounds and stored at -70°C until use. Cells are then seeded into the drug-prepared plates and placed in an incubator for 72 h. On the last day of incubation, the plates are washed and a buffer containing FDA is added and incubated with the cells for 45 minutes. Finally the fluorescence per well is measured in a fluorometer and a Survival Index % (SI) for each compound-treated well is calculated with the equation: Compound-treated cells minus blank divided by control cells minus blank. A high Si-value indicates a large percentage of living cells and vice versa.
For experiments with compounds of the invention, 96-well plates were prepared as follows:
Compounds were dissolved in DMSO to 10 mM and stored at -20°C. 96-well plates were prepared with 297 μΐ of sterile PBS added to each well. The test compounds were thawed, protected from light, mixed, and 3 μΐ of stock solution was added to the 96-well plate to give the concentration 100 μΜ. Then, an assay plate was prepared by transferring 20 μΐ of compound solution to a V-bottomed 96-well plate. Compounds at 100 μΜ were diluted with PBS to 10 μΜ, and an assay plate containing 20 μΐ was prepared. The plates were stored at - 70°C until use.
On the day of cell seeding, 180 μΐ of cell suspension was added to each well in the two assay plates. The final concentration of compounds tested was thus 10 μΜ and 1 μΜ.
In subsequent experiments, compounds were tested, along with kinase inhibitors (dasatinib, pazopanib, sorafenib, and sunitinib) as described above. Initially, the acute lymphoblastic leukaemia cell line CCRF-CEM (see e.g. Foley GE, et al. Cancer 1965, 18, 522-529) was used throughout. In the assay plates, medium was added to six empty wells (blank wells) and wells were filled with PBS and cell suspension and served as control wells. Si-values were then calculated for each compound-treated well as described above. All experiments were conducted twice and a new batch of plates was prepared for each experiment. The data obtained showed the activity of the example compounds compared to the comparative compounds.
For dose-response experiments, 384-well plates were prepared as follows:
Compounds of the invention as well as kinase inhibitors sorafenib, sunitinib, dasatinib, and pazopanib (reference compounds) were diluted with PBS to a concentration which was ten-times higher than the desired starting concentration. Then, a Biomek 2000 liquid handling system was employed to serially dilute the compounds in a deep-well 384-well plate. From this plate, assay plates containing 5 μΐ compound per well were prepared with the Biomek 2000. Certain compounds precipitated when diluted with PBS, and these compounds were therefore prepared in a 96-well plate manually as described above using culture medium RPMI 1640 instead of PBS.
The compounds were also tested at five concentrations, with five times serial dilution on the following cell types: CCRF-CEM, hTERT-RPEl (normal retinal epithelial cells), hRPTEpiC (normal renal cells) and PBMC (peripheral blood mononuclear cells). Each experiment was performed three times, except for PBMC and hRPTEpiC, which were performed twice. Sl-values were calculated, graphs were plotted using GraphPadPrism 5.0 (GraphPad Software Inc. La Jo 11a, CA) and ECso-values for each cell type and compound were determined from the curves.
The example compounds of the invention were active in the CCRF-CEM cell measurements, showing EC50 values less than 10 μΜ. Preferred compounds of the invention had EC50 values less than 1 μΜ. More preferred compounds of the invention had EC50 values less than 0.1 μΜ, and the reference compounds, sorafenib, sunitinib, dasatinib, and pazopanib, had EC50 values of 8.3 μΜ, 14.1 μΜ, 9.7 μΜ, and 25.9 μΜ respectively, in this assay. Most of the compounds of the invention showed lower EC50 values than the reference compounds and data is presented in Table 1.
Table 1. EC50 (50μΜ) in CCRF-CEM cancer cells - leukaemia
Example CCRF-CEM Example CCRF-CEM
number EC50 (μΜ) number EC50 (μΜ)
1 0.33 76 0.23
2 4.83 81 <10
3 4.25 82 0.49
4 4.07 83 <10
9 8.73 84 0.04
10 10.2 85 <10
11 2.88 86 tbt.ndy
12 0.76 87 tbt.ndy
13 0.55 88 tbt.ndy
17 7.28 89 tbt.ndy 18 0.71 90 tbt.ndy
19 1.02 91 tbt.ndy
20 1.01 92 tbt.ndy
23 1.62 93 tbt.ndy
24 0.38 94 tbt.ndy
25 3.16 95 0.90
26 7.01 96 0.68
27 4.17 97 tbt.ndy
28 3.10 98 tbt.ndy
29 2.78 99 tbt.ndy
30 0.30 100 tbt.ndy
32 7.81 101 tbt.ndy
33 9.55 102 tbt.ndy
35 10.1 103 tbt.ndy
37 0.42 104 tbt.ndy
38 4.33 105 tbt.ndy
39 2.84 106 tbt.ndy
40 4.17 107 tbt.ndy
63 0.59 108 tbt.ndy
64 0.05 109 tbt.ndy
65 0.92 Ref. l 8.3
66 0.21 Ref. 2 14.1
67 0.21 Ref. 3 9.7
69 <10 Ref. 4 25.9
70 <10
71 <10
72 <10
73 <10
74 <10
75 0.61 tbt.ndy denotes "to be tested, no data available yet".
Ref.1 denotes reference compound sorafenib
Ref.2 denotes reference compound sunitinib
Ref.3 denotes reference compound dasatinib
Ref.4 denotes reference compound pazopanib
Further, primary results also showed that the compounds of the invention exhibited an enhanced selectivity towards CCRF-CEM cells, compared to the tested hTERT-RPEl (normal retinal epithelial cells), hRPTEpiC (normal renal cells), and peripheral blood mononuclear cells (PBMC).
The compounds of the invention were also tested on further cancer cell lines related to colon cancer (HCT116; see e.g Brattain MG, et al. Cancer Res. 1981, 41, 1751- 1756), breast cancer (MCF7; see e.g. Soule HD, et al. J. Natl. Cancer Inst. 1973, 51, 1409-1416), teniposide-resistant leukaemia (CEM/VM1; see e.g. Danks MK et al. Cancer Res. 1987, 47, 1297-1301), lung cancer (H69; see e.g. Gazdar AF, et al. Cancer Res. 1980, 40, 3502-3507), doxorubicin-resistant lung cancer (H69AR; see e.g. Mirski SE, et al. Cancer Res. 1987, 47, 2594-2598), myeloma (RPMI 8226; see e.g. Matsuoka Y, et al. Proc. Soc. Exp. Biol. Med. 1967, 125, 1246-1250), doxorubicin-resistant myeloma (8226/Dox40; see e.g. Dalton WS et al. Blood 1989, 15,747-752), lymphoma (U-937, see e.g. Sundstrom C, et al. Int. J. Cancer 1976, 17, 565-577), vincristin-resistant lymphoma (U-937-vcr; see e.g. Botling J, et al. Int J Cancer 1994, 15;58 (2), 269-274), ovarian cancer (A2780; see e.g. Hamilton TC, et al. Semin Oncol. 1984, 11, 285-298), doxorubicin-resistant ovarian cancer
(A2780/Adr), cisp latin-resistant ovarian cancer (A2780/Cis; see e.g. Behrens BC, et al. Cancer Res. 1987, 47, 414-418), renal cancer (ACHN; see e.g. Borden EC, et al. Cancer Res. 1982, 42(12), 4948-4953), pancreatic cancer (PANC-1, BxPC-3, and MIA PaCa-2; see e.g. Lieber M, et al. Int. J. Cancer 1975, 15, 741-747; Loor R, et al. Clin. Lab. Med. 1982, 2, 567-578; and Yunis AA, et al. Int. J. Cancer 1977, 19, 128- 135). Representative results of these tests are presented in Table 2 and Table 3.
Table 2. EC50 (μΜ) in various cancer cell lines
CEM/ RPMI 8226/
Ex. HCT116 MCF7 H69 H69 AR ACHN
VM1 8226 Dox40
1 6.70 9.0 0.12 10.8 nt 0.50 0.37 2.54
12 0.63 3.88 0.17 7.84 0.27 0.53 1.81 1.90
19 9.83 28.5 1.33 30.7 nt 12.80 46.3 25.2
24 0.51 4.43 0.21 9.35 0.46 0.23 0.41 1.26
30 2.62 11.0 0.35 7.14 nt 0.34 0.34 3.30 37 6.20 4.92 0.23 11.6 nt 0.81 2.02 3.08
64 1.33 2.33 0.05 4.18 0.29 0.23 1.19 1.60
66 2.45 3.35 0.27 11.6 0.64 1.83 10.0 3.32
67 2.47 12.4 0.33 37.2 0.71 5.01 24.4 5.43
76 3.09 3.08 0.22 12.7 0.34 0.70 1.10 1.59
84 1.16 2.06 0.19 5.47 0.35 0.45 2.58 1.62
"nt" denotes "not yet tested".
Table 3. EC50 (μΜ) in various cancer cell lines
Figure imgf000113_0001
"nt" denotes "not yet tested". Example compounds were further tested in a tubulin polymerization assay kit from Cytoskeleton Inc (Denver, CO, USA). Polymerization is followed by fluorescence enhancement due to the incorporation of a fluorescent reporter into microtubules as polymerization occurs. Vincristine and paclitaxel at 3 μΜ were used as positive controls for tubulin polymerization inhibition and stabilisation, respectively. All compounds were dissolved in DMSO, which was used as solvent control. For experiments, example compounds and control compounds were incubated with bovine tubulin protein in a cell- free environment and the fluorescence was then measured in a fluorometer (Fluostar Optima, BMG Labtech, Offenburg, Germany) at 360/450 nm every minute for a total of 60 mins. Some selected example compounds showed inhibitory effects on tubulin polymerization.
Example compounds were further tested for induction of apoptosis in a Live cell imaging setup. The NucView™ 488 Caspase-3 Assay Kit for Live cells (Biotium, Inc. Hayward, CA, USA) was used. HCT116 cells were plated the day before the experiment in black glass-bottom PerkinElmer plates and compounds with chosen concentrations were then added. Finally, the DEVD-NucView488 Caspase-3 substrate was added and the plate was placed in an IncuCyteFLR for live-cell imaging. When the substrate is cleaved by activated caspase-3, a dye is released which becomes fluorescent upon binding to DNA (Cen H et al. DEVD-NucView488: a novel class of enzyme substrates for real-time detection of caspase-3 activity in live cells. FASEB J. 2008 Jul: 22(7):2243-52.). Staurosporin at 1 μΜ was used as a positive control for apoptosis. Selected example compounds were tested and all of them induced apoptosis at the chosen concentrations at varying time points.

Claims

Claims
1. A compound of formula I or a pharmaceutically acceptable ester, amide, solvate or salt thereof,
Figure imgf000115_0001
wherein
Z represents carbon or nitrogen;
Y represents carbon or nitrogen, wherein one of Z and Y represents nitrogen; A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and D and E represents carbon; or A and D represent nitrogen and E represents carbon; or A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl, when D represents carbon;
R2 represents hydrogen or amino, when Y or Z represents carbon;
R3 represents hydrogen, (Ci-C3)alkyl, amino, trifluoromethyl or (C0- Ci)alkylaryl;
R4 represents heteroaryl, optionally substituted with one or more substituents; and
R5 represents hydrogen or methyl;
provided that the compound N2,N4-bis(lH-indol-5-ylmethyl)pyrimidine-2,4- diamine is excluded. A compound according to claim 1 , wherein Z, D and E represent carbon; and Y and A represents nitrogen.
A compound according to claim 1 , wherein Z and E represent carbon; and Y, D and A represent nitrogen.
A compound according to claim 1 , wherein Z and D represent carbon; and Y, E and A represent nitrogen.
A compound according to claim 1, wherein Z, A and D represent carbon; and Y and E represent nitrogen.
A compound according to claim 1, wherein Y, D and E represent carbon; and Z and A represent nitrogen.
A compound according to claim 1 , wherein Y and E represent carbon; and Z, D and A represent nitrogen.
A compound according to claim 1, wherein Y and D represent carbon; and Z, E and A represent nitrogen.
A compound according to claim 1 , wherein Y, A and D represent carbon; and Z and E represent nitrogen.
A compound according to any one of claims 1 to 9, wherein R4 represents a heteroaryl that is optionally substituted with one or more substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO) H(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci- C4)alkyl-0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci- C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-0(CO)(C6- Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci- C4)alkyl] [(C i -C4)-alkyl] , (C i -C4)alkyl-NH(CO)(C i -C4)alkyl, (C i -C4)alkyl- NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(CO)(C6-Ci0)aryl, (Ci- C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci- C4)alkyl, (CO)(Ci-C4)alkyl(C6-Cio)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6- Cio)aryl, (CO)(Ci-C9)heteroaryl, (C6-Cio)aryl, (C6-Cio)aryl- halogen, (C6- Cio)aryl-OH, (C6-Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci- C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2- C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci- C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, 0(Ci- C4)alkyl(C6-Cio)aryl, O(Ci-C4)alkyl(C6-Ci0)heteroaryl, 0(Ci-C4)alkyl(C2- C9)heterocyclyL 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci- C4)alkyl, 0(C6-Cio)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2, 0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3.
A compound according to any one of claims 1 to 10, wherein L-R4 is selected from:
Figure imgf000117_0001
Figure imgf000118_0001
Figure imgf000118_0002
wherein R6 is selected from hydrogen and (Ci-C4)alkyl;
R7 is selected from hydrogen, halogen, nitro, cyano, (Ci-C4)alkyl, (Ci- C4)alkyl(CO)OH, (Ci-C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (d- C4)alkyl(CO)NH(Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci-C4)alkyl-0(C6-Cio)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-0(CO)(C6-Cio)aryl, (Ci-
C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci- C4)alkyl], (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl(CN), (Ci- C4)alkyl(C6-Cio)aryl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)OH, (CO)O(Ci- C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)(Ci-C4)alkyl, (C6-Ci0)aryl, (C6- Cio)aryl-halogen, (C6-Ci0)aryl-OH, (C6-Cio)aryl-0(Ci-C4)alkyl, (Ci-
C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci- C9)heteroaryl-0(Ci-C4)alkyl, (C2-C9)heterocyclyl, (C2-C9)heterocyclyl(Ci- C4)alkyl, and (C2-C9)heterocyclyl(Ci-C4)alkyl-OH;
R7a and R7b are independently selected from hydrogen and (Ci-C4)alkyl, preferably methyl;
R8 is selected from hydrogen, halogen, nitro, cyano, hydroxy, amino, (Ci- C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl- NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci-C4)alkyl- NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(C6-Cio)aryl, (CO)(Ci-C4)alkyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, 0(Ci-C4)alkyl,
0(Ci-C4)alkyl(C6-Cio)aryl, 0(Ci-C4)alkyl(Ci-C9)heteroaryl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci- C4)alkyl, 0(C6-Cio)aryl, OCF3, 0(CO)(Ci-C4)alkyl, O(CO)(C6-Ci0)aryl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci- C4)alkyl, NH(CO)(C6-Ci0)aryl, CF3, (C6-Ci0)aryl, (C6-Cio)aryl-halogen, (C6-
Cio)aryl-OH, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2- C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, and (C2-C9)heterocyclyl(Ci- C4)alkyl-OH;
R9 is selected from hydrogen, halogen, (Ci-C4)alkyl, (Ci-C4)alkyl-OH, (Ci-
C4)alkyl-0(Ci-C4)alkyl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci- C4)alkyl, and (C6-Cio)aryl; and
R1 1 is selected from hydrogen, hydroxy, (Ci-C4)alkyl, and 0(Ci-C4)alkyl.
A compound according to any one of claims 1 to 11 , wherein R5 represents hydrogen.
13. A compound according to any one of claims 1 to 11 , wherein R5 represents methyl.
14. A compound according to any one of claims 1 to 13, wherein R2 represents amino.
15. A compound according to any one of claims 1 to 13, wherein R2 represents hydrogen.
16. A compound according to any one of claims 1 to 13, wherein R2 represents hydrogen; and R3 represents hydrogen, methyl, trifluoromethyl or benzyl. 17. A compound according to claim 1 , wherein
Z, D and E represent carbon; Y and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci-
C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci- C4)alkyl-0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci- C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-0(CO)(C6- Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci- C4)alkyl][(Ci-C4)-alkyl], (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-
NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(CO)(C6-Ci0)aryl, (Ci- C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci- C4)alkyl, (CO)(Ci-C4)alkyl(C6-Cio)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6-
Cio)aryl, (CO)(Ci-C9)heteroaryl, (C6-Cio)aryl, (C6-Cio)aryl- halogen, (C6- Cio)aryl-OH, (C6-Ci0)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci- C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2- C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci-
C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, 0(Ci- C4)alkyl(C6-Cio)aryl, O(Ci-C4)alkyl(C6-Ci0)heteroaryl, 0(Ci-C4)alkyl(C2- C9)heterocyclyL 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci- C4)alkyl, 0(C6-Cio)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2,
0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-C4)alkyl, N[(C i -C4)alkyl] [(C i -C4)alkyl] , NH(CO)(C i -C4)alkyl, NH(CO)(C i -C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and R5 represents hydrogen or methyl.
A compound according to claim 1 , wherein
Z and E represent carbon;
Y, D, and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci- C4)alkyl-0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci- C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-0(CO)(C6- Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci- C4)alkyl] [(C i -C4)-alkyl] , (C i -C4)alkyl-NH(CO)(C i -C4)alkyl, (C i -C4)alkyl- NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(CO)(C6-Ci0)aryl, (Ci- C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci- C4)alkyl, (CO)(Ci-C4)alkyl(C6-Cio)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6- Cio)aryl, (CO)(Ci-C9)heteroaryl, (C6-Cio)aryl, (C6-Cio)aryl- halogen, (C6- Cio)aryl-OH, (C6-Ci0)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci- C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2- C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci- C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, 0(Ci- C4)alkyl(C6-Cio)aiyl, O(Ci-C4)alkyl(C6-Ci0)heteioaiyl, 0(Ci-C4)alkyl(C2- C9)heterocyclyL 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci- C4)alkyl, 0(C6-Cio)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2, 0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-C4)alkyl,
N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and R5 represents hydrogen or methyl. 19. A compound according to claim 1, wherein
Z and D represent carbon;
Y, E and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-
C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci- C4)alkyl-0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci- C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-0(CO)(C6- Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-
C4)alkyl][(Ci-C4)-alkyl], (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl- NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(CO)(C6-Ci0)aryl, (Ci- C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci- C4)alkyl, (CO)(Ci-C4)alkyl(C6-Cio)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl,
(CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6- Cio)aryl, (CO)(Ci-Cc))heteroaryl, (C6-C10)aryl, (C6-Cio)aryl- halogen, (C6- Cio)aryl-OH, (C6-Cio)aryl-NH2, (C6-Cio)aryl-0(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl- halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci- C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2- C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci- C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, 0(Ci- C4)alkyl(C6-Cio)aryl, O(Ci-C4)alkyl(C6-Ci0)heteroaryl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci- C4)alkyl, 0(C6-Cio)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2, 0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-C4)alkyl, N[(C i -C4)alkyl] [(C i -C4)alkyl] , NH(CO)(C i -C4)alkyl, NH(CO)(C i -C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and R5 represents hydrogen or methyl.
A compound according to claim 1 , wherein
Y, D and E represent carbon;
Z and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen or methyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci- C4)alkyl-0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci- C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-0(CO)(C6- Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci- C4)alkyl] [(C i -C4)-alkyl] , (C i -C4)alkyl-NH(CO)(C i -C4)alkyl, (C i -C4)alkyl- NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(CO)(C6-Ci0)aryl, (Ci- C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci- C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl,
(CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6- Cio)aryl, (CO)(Ci-C9)heteroaryl, (C6-Cio)aryl, (C6-Cio)aryl- halogen, (C6- Cio)aryl-OH, (C6-Cio)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci- C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2-
C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci- C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, 0(Ci- C4)alkyl(C6-Cio)aryl, O(Ci-C4)alkyl(C6-Ci0)heteroaryl, 0(Ci-C4)alkyl(C2- C9)heterocyclyL 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci-
C4)alkyl, 0(C6-Cio)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2, 0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and R5 represents hydrogen or methyl.
A compound according to claim 1 , wherein
Y and E represent carbon;
Z, D, and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (Ci-C4)alkyl(C2-C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci- C4)alkyl(CO)0(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci- C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci- C4)alkyl-0(Ci-C4)alkyl, (Ci-C4)alkyl-O(C6-Ci0)aryl, (Ci-C4)alkyl-0(CO)(Ci- C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-0(CO)(C6- Cio)aryl, (Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci- C4)alkyl] [(C i -C4)-alkyl] , (C i -C4)alkyl-NH(CO)(C i -C4)alkyl, (C i -C4)alkyl- NH(CO)(Ci-C4)alkyl-NH2, (Ci-C4)alkyl-NH(CO)(C6-Ci0)aryl, (Ci- C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-C4)alkyl(CO)OH, (CO)(Ci- C4)alkyl, (CO)(Ci-C4)alkyl(C6-Cio)aryl, (CO)(Ci-C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2-C9)heterocyclyl, (CO)(C6- Cio)aryl, (CO)(Ci-C9) eteroaryl, (C6-Cio)aryl, (C6-Cio)aryl- halogen, (C6- Cio)aryl-OH, (C6-Ci0)aryl-NH2, (C6-Ci0)aryl-O(Ci-C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH, (Ci-C9)heteroaryl-NH2, (Ci- C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci-C4)alkyl, (C2- C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2-C9)heterocyclyl(Ci- C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci-C4)alkyl, 0(Ci- C4)alkyl(C6-Cio)aryl, O(Ci-C4)alkyl(C6-Ci0)heteroaryl, 0(Ci-C4)alkyl(C2- C9)heterocyclyl 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci- C4)alkyl, 0(C6-Cio)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2, 0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and R5 represents hydrogen or methyl.
A compound according to claim 1 , wherein
Y and D represent carbon;
Z, E and A represent nitrogen;
L represents a bond or (Ci-C2)alkyl;
R1 represents hydrogen; R2 represents hydrogen;
R3 represents hydrogen or methyl;
R4 represents a heteroaryl selected from indolyl, indazolyl, benzimidazolyl, and indolinonyl, said heteroaryl optionally substituted with one or two substituents selected from the group consisting of
halogen, hydroxy, amino, nitro, cyano, (Ci-C4)alkyl, (C1-C4)alkyl(C2- C9)heterocyclyl, (Ci-C4)alkyl(CO)OH, (Ci-C4)alkyl(CO)0(Ci-C4)alkyl, (Ci- C4)alkyl(CO)NH2, (Ci-C4)alkyl(CO)NH(Ci-C4)alkyl, (Ci-C4)alkyl(CO)NH(Ci- C4)alkyl(CO)OH, (Ci-C4)alkyl-OH, (Ci-C4)alkyl-0(Ci-C4)alkyl, (Ci-C4)alkyl- 0(C6-Cio)aryl, (Ci-C4)alkyl-0(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-0(CO)(Ci-
C4)alkyl-NH2, (Ci-C4)alkyl-O(CO)(C6-Ci0)aryl, (Ci-C4)alkyl-NH2, (Ci- C4)alkyl-NH(Ci-C4)alkyl, (Ci-C4)alkyl-N[(Ci-C4)alkyl][(Ci-C4)-alkyl], (Ci- C4)alkyl-NH(CO)(Ci-C4)alkyl, (Ci-C4)alkyl-NH(CO)(Ci-C4)alkyl-NH2, (d- C4)alkyl-NH(CO)(C6-Cio)aryl, (Ci-C4)alkyl(CN), (Ci-C4)alkyl(C6-Ci0)aryl, (CO)OH, (CO)0(Ci-C4)alkyl, (CO)NH2, (CO)NH(Ci-C4)alkyl, (CO)NH(Ci-
C4)alkyl(CO)OH, (CO)(Ci-C4)alkyl, (CO)(Ci-C4)alkyl(C6-Ci0)aryl, (CO)(Ci- C4)alkyl(Ci-C9)heteroaryl, (CO)(Ci-C4)alkyl(C2-C9)heterocyclyl, (CO)(C2- C9)heterocyclyl, (CO)(C6-Ci0)aryl, (CO)(Ci-C9)heteroaryl, (C6-Ci0)aryl, (C6- Cio)aryl-halogen, (C6-Ci0)aryl-OH, (C6-Ci0)aryl-NH2, (C6-Ci0)aryl-O(Ci- C4)alkyl, (Ci-C9)heteroaryl, (Ci-C9)heteroaryl-halogen, (Ci-C9)heteroaryl-OH,
(Ci-C9)heteroaryl-NH2, (Ci-C9)heteroaryl(Ci-C4)alkyl, (Ci-C9)heteroaryl-0(Ci- C4)alkyl, (C2-C9)heterocyclyl, (C2-C9)heterocyclyl(Ci-C4)alkyl, (C2- C9)heterocyclyl(Ci-C4)alkyl-OH, (C2-C9)heterocyclyl(Ci-C4)alkyl-NH2, 0(Ci- C4)alkyl, 0(Ci-C4)alkyl(C6-Cio)aryl, O(Ci-C4)alkyl(C6-Ci0)heteroaryl, 0(Ci- C4)alkyl(C2-C9)heterocyclyl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl,
0(Ci-C4)alkyl(C2-C9)heterocyclyl(Ci-C4)alkyl-OH, 0(EtO)i_3H, 0(EtO)i_3(Ci- C4)alkyl, 0(C6-Cio)aryl, 0(CO)(Ci-C4)alkyl, 0(CO)(Ci-C4)alkyl-NH2, 0(CO)(C6-Cio)aryl, OCF3, OS02(Ci-C4)alkyl, OS02OH, NH(Ci-C4)alkyl, N[(Ci-C4)alkyl][(Ci-C4)alkyl], NH(CO)(Ci-C4)alkyl, NH(CO)(Ci-C4)alkyl- NH2, NH(CO)(C6-Cio)aryl, NHS02(Ci-C4)alkyl, S02NH2, and CF3; and
R5 represents hydrogen or methyl.
23. A compound according to claim 1 , wherein
Z represents carbon or nitrogen;
Y represents carbon or nitrogen, wherein one of Z and Y represents nitrogen;
A, D and E is selected from carbon and nitrogen, wherein A represents nitrogen and D and E represents carbon; or A and D represent nitrogen and E represents carbon; or A and E represent nitrogen and D represents carbon; or E represents nitrogen and A and D represent carbon;
R1 represents hydrogen or methyl, when D represents carbon;
R2 represents hydrogen or amino;
R3 represents hydrogen, methyl, trifluoromethyl or (Co-Ci)alkylaryl; R5 represents hydrogen or methyl;
L-R4 is selected from:
Figure imgf000127_0001
selected from hydrogen and methyl; R7 is selected from hydrogen, methyl, (Ci-C4)alkyl-OH, and (CO)OCH3, R7a and R7b are independently selected from hydrogen, and methyl;
R8 is selected from halogen, hydrogen, hydroxy, (CO)OH, (CO)OCH3, 0(Ci- C4)alkyl, 0(Ci-C4)alkyl(C6-Cio)aryl, 0(Ci-C4)alkyl(C2-C9)heterocyclyl, 0(EtO)i_3(Ci-C4)alkyl, and OCF3;and
R1 1 is selected from hydrogen, methyl, and 0(Ci-C4)alkyl.
24. A compound according to claim 1 , wherein L represents a bond or (C2)alkyl. 25. A compound according to claim 1 , said compound being selected from:
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-5-yl)pyrimidine-2,4-diamine;
N2 2-(lH-indol-3-yl)ethyl]-A^-(lH-indol-5-ylmethyl)pyrimidine-2,4-diamine;
3 - {2- [4-( lH-indo 1-5 -ylmethylamino)-pyrimidin-2-ylamino] ethyl} - lH-indo 1-5 - ol;
A^-(lH-indol-5-ylmethyl)-N2-[2-(5-methyl-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]pyrimidine- 2,4-diamine;
N2-(lH-indol-4-yl)-N4-(2 -methyl- lH-indol-5ylmethyl)pyrimidine-2,4-diamine;
N2-(2-(lH-indol-3-yl)-ethyl)-N4-(2-methyl-lH-indol-5ylmethyl)pyrimidine-2,4- diamine;
N2-( lH-indol-4-yl)-N4-( lH-indazol-5ylmethyl)pyrimidine-2,4-diamine;
N4-(lH-benzo[<i]imidazol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4- diamine;
N4-(lH-indol-6-ylmethyl)-N -(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-diamine;
N4-[2-(lH-indol-3-yl)ethyl]-N2-(lH-indol-5-ylmethyl)pyrimidine-2,4-diamine; 3 - {2- [2-( lH-indo 1-5 -ylmethylamino)-pyrimidin-4-ylamino] ethyl} - lH-indo 1-5 - ol;
N2-( lH-indol-5 -ylmethyl)-^4- [2-(5 -methyl- lH-indol-3 -yl)ethyl]pyrimidine-2,4 diamine;
N2-( lH-indol-5 -ylmethyFj-N4- [2-(5 -methoxy- lH-indol-3 -yl)ethyl]pyrimidine- 2,4-diamine;
N2-(lH-indazol-5-ylmethyl)-N4-(lH-indol-4-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-4-yl)-N4-(lH-indol-5ylmethyl)-6-methylpyrimidine-2,4-diamine; 3 - {2- [4-( lH-indo 1-5 -ylmethylamino)-6-methyl-pyrimidin-2-ylamino] -ethyl} - lH-indol-5-ol;
N4-(lH-indol-5-ylmethyl)-N2-(2-methyl-lH-indol-5-yl)-6- trifluoromethylpyrimidine-2,4-diamine;
N2-(2-(lH-indol-3-yl)ethyl)-N4-(lH-indol-5-ylmethyl)-6- trifluoromethylpyrimidine-2,4-diamine;
N4-(lH-indazol-5-ylmethyl)-N2-(lH-indol-4-yl)-6-methylpyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-4-yl)pyrimidine-2,4,5-triamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-5-yl)pyrimidine-2,4,5-triamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indol-6-yl)pyrimidine-2,4,5-triamine;
^-(2 -methyl- 1H- indol-5-yl)-N2-(2 -methyl- lH-indo l-5-ylmethyl)pyrimidine- 2,4-diamine;
N2-[2-(5-methoxy- lH-indol-3-yl)ethyl]-N4-(2-methyl- lH-indol-5- ylmethyl)pyrimidine-2,4-diamine;
N2-(lH-indazol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4- diamine;
N4-( lH-indazol-5 -ylmethyl)-N2- [2-(5 -methoxy- lH-indol-3 -yl)ethyl]pyrimidine 2,4-diamine;
N2-(lH-benzo[ ]imidazol-5-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine 2,4-diamine;
N*-( lH-benzo [ ]imidazol-5 -ylmethyl)-N2- [2-(5 -methoxy- lH-indol-3 - yl)ethyl]pyrimidine-2,4-diamine; N2-(lH-indol-6-ylmethyl)-N4-(2-methyl-lH-indol-5-yl)pyrimidine-2,4-d^
N4-(lH-indol-6-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]pyrimidine-
2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2- {2-[5-(benzyloxy)-lH-indol-3- yl]ethyl}pyrimidine-2,4-diamine;
A^-(lH-indol-5-ylmethyl)-N2- {2-[5-(2-morpholinoethoxy)-lH-indol-3- yl]ethyl}pyrimidine-2,4-diamine;
N*-( lH-indol-5 -ylmethyl)-N2- {2- [5 -(2-methoxyethoxy)- lH-indol-3 - yl]ethyl}pyrimidine-2,4-diamine;
N4-( lH-indol-5-ylmethyl)-N2-( 1 -methyl- lH-indol-4-yl)pyrimidine-2,4-diamine; N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-4-yl)pyrimidine-2,4-diamine;
Methyl 4- [4-( lH-indo 1-5 -ylmethylamino)pyrimidin-2-ylamino] - lH-indo le-6- carboxylate;
N2-(lH-indol-5-ylmethyl)-N4-(lH-indol-5-yl)pyrimidine-2,4-diamine;
N2-(lH-indol-5-ylmethyl)-6-methyl-N4-(2 -methyl- lH-indo l-5-yl)pyrimidine- 2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]-6- methylpyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-6-benzyl-N2-[2-(5-methoxy-lH-indol-3- yl)ethyl]pyrimidine-2,4-diamine;
N*-( lH-indol-5 -ylmethyl)-N2- [2-(5 -methoxy-7-methyl- 1 H-indol-3 - yl)ethyl]pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-ethoxy-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
N*-( lH-indol-5 -ylmethyl)-N2- {2- [5 -(trifluoromethoxy)- lH-indol-3 - yl]ethyl}pyrimidine-2,4-diamine;
A^-(lH-indol-5-ylmethyl)-N2-[2-(5-fluoro-lH-indol-3-yl)ethyl]pyrimidine-2,4- diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(6-methoxy-lH-indol-3-yl)ethyl]pyrimidine- 2,4-diamine; N4-(lH-indol-5-ylmethyl)-N2-[2-(7-methoxy-lH-indol-3-yl)ethyl]pyrimidine- 2,4-diamine;
N2-(lH-indol-5-ylmethyl)-A^-(l,2-dimethyl H-indol-5-yl)pyrimidine-2,4- diamine;
methyl 5-[2-(lH-indol-5-ylmethylamino)pyrimidin-4-ylamino]-lH-indole-2- carboxylate;
N2-(lH-indol-5-ylmethyl)-A^-(2,3-dimethyl H-indol-5-yl)pyrimidine-2,^ diamine;
N2-(lH-indol-5-ylmethyl)-N4-(lH-benzo[(i]imidazol-5-yl)pyrimidine-2,4- diamine;
N2-(lH-indol-5-ylmethyl)-N4-(2-methyl-lH-benzo[(i]imidazol-5-yl)pyrimidine- 2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-(lH-indazol-4-yl)-6-methylpyrimidine-2,4- diamine;
N2- [2-(5-methoxy- lH-indol-3 -yl)ethyl] -6-methyl-N4- [(2-methyl- lH-indol-5 - yl)methyl]pyrimidine-2,4-diamine;
N*-( lH-indazol-5 -ylmethyl)-N2- [2-(5 -methoxy- lH-indol-3 -yl)ethyl] -6- methylpyrimidine-2,4-diamine;
N*-( lH-indol-5 -ylmethyl)-N2- [2-(5 -methoxy-2-methyl- 1 H-indol-3 -yl)ethyl] -6- methylpyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(4-methoxy-lH-indol-3-yl)ethyl]pyrimidine- 2,4-diamine;
4- [4-( lH-indo 1-5 -ylmethylamino)pyrimidin-2-ylamino] - lH-indo le-6-carboxylic acid;
N2-(lH-indol-4-yl)-6-methyl-N4-[(2-methyl-lH-indol-5-yl)methyl]pyrimidine- 2,4-diamine;
{5 - [2-( lH-indo 1-5 -ylmethylamino)pyrimidin-4-ylamino] - lH-indo 1-2- yl} methanol;
N2-(lH-indol-5-ylmethyl)-N4-methyl-N4-(2-methyl-lH-indol-5-yl)pyrimidine- 2,4-diamine; ^-(lH-indol-S-ylmethy -A^-il^-dimethyl-lH-indol-S-y -N4- methylpyrimidine-2,4-diamine;
N*-( lH-indol-5 -ylmethyl)-N2- [2-(5 -methoxy- 1 -methyl- 1 H-indol-3 - yl)ethyl]pyrimidine-2,4-diamine;
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-l-methyl-lH-indol-3-yl)ethyl]-N2- methylpyrimidine-2,4-diamine; and
N4-(lH-indol-5-ylmethyl)-N2-[2-(5-methoxy-lH-indol-3-yl)ethyl]-N2- methylpyrimidine-2,4-diamine. 26. A compound according to any one of claims 1 to 25, for use in therapy.
27. A compound according to any one of claims 1 to 25, for use in treatment of cancer. 28. Use of a compound according to any one of claims 1 to 25, in the manufacture of a medicament and pharmaceutical compositions for treatment of cancer.
29. A pharmaceutical composition comprising a compound according to any one of claims 1 to 25 together with pharmaceutically acceptable diluents and carriers.
30. A method for treatment of cancer, which comprises administering to a subject in need thereof, a therapeutically effective amount of a compound according to claim 1. 31. A method for treatment of cancer, which comprises administering to a subject in need thereof, a therapeutically effective amount of a compound according to claim 1, in combination with another compound according to claiml, in combination with radiation therapy, or in combination with another anticancer agent selected from alkylating agents, antimetabolites, anticancer camptothecin derivatives, plan-derived anticancer agents, antibiotics, enzymes, platinum coordination complexes, tyrosine kinase inhibitors, hormones, hormone antagonists, monoclonal antibodies, interferons, and biological response modifiers.
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