WO2011115306A1 - Agent améliorant le rendement d'utilisation d'aliments pour animaux ruminants - Google Patents

Agent améliorant le rendement d'utilisation d'aliments pour animaux ruminants Download PDF

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WO2011115306A1
WO2011115306A1 PCT/JP2011/057270 JP2011057270W WO2011115306A1 WO 2011115306 A1 WO2011115306 A1 WO 2011115306A1 JP 2011057270 W JP2011057270 W JP 2011057270W WO 2011115306 A1 WO2011115306 A1 WO 2011115306A1
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feed
ruminant
utilization efficiency
improving
bacillus subtilis
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PCT/JP2011/057270
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English (en)
Japanese (ja)
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久雄 板橋
寛和 今林
博 宮崎
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カルピス株式会社
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system

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  • the present invention relates to feed utilization efficiency and milk yield improvement of an animal having a ruminant stomach (hereinafter referred to as “ruminant animal”). More specifically, in ruminants such as cattle, sheep and goats, rumen fermentation, generally referred to as rumen fermentation (hereinafter also referred to as “lumen fermentation”), and useful bacteria in the body are improved. Improving the feed digestibility and reducing energy loss, improving the feed utilization efficiency, and as a result, improving the feed utilization efficiency of ruminants that can produce healthy livestock with improved milk yield, The present invention relates to a method for improving feed utilization efficiency, a breeding method, and a ruminant feed.
  • ruminant animal More specifically, in ruminants such as cattle, sheep and goats, rumen fermentation, generally referred to as rumen fermentation (hereinafter also referred to as “lumen fermentation”), and useful bacteria in the body are improved. Improving the feed digestibility and reducing energy loss, improving the feed utilization efficiency, and as a result, improving the
  • the feed digestibility referred to in the present invention is usually referred to as DM (DRY MATTER) digestibility.
  • feed digestibility (%) “1- (feces solid weight) / ( Feed solids weight) ” ⁇ 100.
  • This DM digestibility does not reflect the components that are decomposed and discharged as gas, the components discharged into urine, etc. (loss components). In this invention, the whole efficiency including these loss components (energy loss) is called feed utilization efficiency.
  • Ruminants have special digestion and absorption mechanisms and energy metabolism mechanisms different from those of monogastric animals such as pigs. Therefore, breeding and management require special knowledge and techniques. And in ruminant animals, such as cattle, sheep, and goats, where milk is also a commodity, poor health and reduced milk yield due to illness are serious economic problems for dairy farmers. Moreover, even if the animal is in good physical condition, it is preferable that the amount of milk per head increases because this will lead to an increase in the income of dairy farmers. Furthermore, it is more preferable if such an effect can be obtained efficiently with less bait.
  • microorganisms in the rumen or rumen (bacteria and protozoa (protozoa is called protozoa or protozoa); hereinafter also referred to as “microorganisms in rumen”) produce essential amino acids. Therefore, there has been no need to supplement the feed with essential amino acids and the like.
  • microorganisms in rumen bacteria and protozoa (protozoa is called protozoa or protozoa); hereinafter also referred to as “microorganisms in rumen”
  • protein and essential amino acids need to be supplied for stable production of high milk yield and health maintenance.
  • dairy cows have tried to add cereals, fish meal, blood meal, etc., which are protein sources, as well as various nutrients as feed additives. There was a problem of load on the product, and sufficient effects could not be obtained.
  • Patent Document 2 As a method for increasing milk yield, addition of a protected amino acid that is not decomposed in rumen to the feed has been studied (Patent Document 2), but the adjustment of the addition time is complicated, and the absorption of the protected amino acid is poor. The effect was not sufficient.
  • Patent Document 1 a liquid composition containing an amino acid is orally administered to ruminants (Patent Document 1), but there are problems with the degradation, administration method, and feeding method of amino acids in the liquid composition. .
  • bacillus subtilis is administered to domestic animals to suppress the growth of pathogenic bacteria in the domestic animals, and further to improve the weight gain and feed requirement of the animals.
  • Patent Document 3 the effect of increasing the weight of a combination feed for young pigs containing live cells of Bacillus subtilis C-3102 as an active ingredient is known (Patent Document 3), and further, Bacillus subtilis JA-ZK.
  • a feed additive comprising a strain as an active ingredient is capable of suppressing the growth of pathogenic bacteria in a domestic animal, improving the weight of the animal, and improving the feed demand rate (Patent Document 4), and Bacillus subtilis (Bacillus).
  • subtilis Using an inhibitor containing C-3102 strain and Bacillus subtilis AT-3 strain containing Bacillus subtilis as an active ingredient, mainly in the body of birds
  • Salmonella Salmonella
  • Patent Documents 5 and 6 there is no specific disclosure about use as a feed utilization efficiency improving agent or a milk yield improving agent for ruminants having a unique digestion and absorption mechanism different from pigs and birds.
  • bacteria other than the microorganisms in the rumen grow abnormally, and this can move into the blood and cause gastrointestinal tract diseases, liver disorders, musculoskeletal disorders, reproductive disorders, heat stroke, mastitis, dermatitis, etc. There was a problem.
  • Non-Patent Document 1 The specific digestion and absorption mechanism and energy metabolism mechanism of ruminants such as cattle are well-known technical matters (Non-Patent Document 1), and their features will be briefly described here.
  • monogastric animals such as pigs and horses
  • the ingested feed is sequentially decomposed by enzymes secreted by itself when it is sequentially transported to the oral cavity, esophagus, stomach, small intestine, and large intestine (for example, starch is converted into glucose). Absorbed) from the digestive tract wall.
  • Monogastric animals such as pigs, including humans, are called sugar animals because they seek sugar as an energy source.
  • ruminants such as cattle have different digestion and absorption mechanisms than monogastric animals.
  • the rumen fermentation is called rumen fermentation, which is characterized by the production of volatile fatty acids (VFA) from carbohydrate sources such as plant fibers and the resynthesis of proteins. Once swallowed, the roughage is returned to the mouth and re-chewed (rubbed), and again undergoes rumen fermentation in the rumen.
  • the rumen is called a so-called “continuous fermentation tank” and is home to a wide variety of anaerobic microorganisms (bacteria and protozoa), and ruminants live with these microorganisms to digest carbohydrates. Microorganisms decompose these to produce volatile fatty acids (VFA) such as acetic acid, propionic acid and butyric acid.
  • VFA volatile fatty acids
  • fatty acids are absorbed directly from the rumen wall and used as raw materials for milk components and other final digested products, and more than 60% of the energy consumed by body tissues and about half of the fuel used to assemble milk components in the breast. It is a source of vitality to cover.
  • VFA volatile fatty acids
  • the most energy efficient is propionic acid. Ruminants such as cows are called fat-proof animals that seek energy sources from fatty acids.
  • protein in feed is hardly digested in the stomach and is carried to the intestine, where it is decomposed into amino acids by the action of the digestive enzyme of the animal itself and then absorbed.
  • ruminants such as cattle are decomposed into amino acids and then into ammonia by the action of the proteolytic enzymes of the microorganisms in the rumen, and the microorganisms are newly made amino acids necessary for themselves, that is, microorganisms with high nutritional value.
  • Synthesize body protein bacterial protein, insect protein).
  • the resulting “microbial protein” is transported along with the stomach contents to the fourth and subsequent stomachs, and follows the process of being decomposed and absorbed into amino acids mainly in the small intestine, as in monogastric animals.
  • a bypass protein that is transported to the small intestine without being decomposed by microorganisms in the lumen and digested there.
  • This is called metabolic protein together with microbial protein, but these are converted to amino acids in the small intestine and absorbed.
  • the rumen is a so-called live fermentation device that supplies the necessary energy sources, while methane, carbon dioxide, nitrogen and small amounts of oxygen, hydrogen, hydrogen sulfide, etc. are byproducts of the fermentation. Most of them are released into the atmosphere as gas without being used, resulting in energy loss.
  • the nutrient in feed is discharged
  • one-third of the nitrogen ingested from the feed is excreted in feces and one-third in urine, and the rest is transferred into milk or accumulated in the body.
  • microbial organisms that improve rumen fermentation, increase and activate rumen microorganisms, improve energy-efficient propionic acid production, and have a high nutritional protein source. It is important to improve protein synthesis, improve feed digestibility, reduce energy loss, and increase feed utilization efficiency, and development of such effective ruminant feed utilization improvement agents is required.
  • an object of the present invention is to provide a feed utilization efficiency improving agent for ruminants.
  • an object of the present invention is to provide a feed utilization efficiency improving agent for ruminant-containing solid feed.
  • this invention aims at providing the usage method of a feed utilization efficiency improving agent characterized by using a feed utilization efficiency improving agent as a milk yield improving agent.
  • Another object of the present invention is to provide a method for breeding ruminants, characterized in that a feed utilization efficiency improving agent is fed directly to ruminants or added to feed.
  • the inventors of the present application have improved the specific digestive absorption mechanism and energy metabolism mechanism of ruminants by using viable cells of Bacillus subtilis as an active ingredient. Rumen fermentation of ruminants is improved to promote the increase and / or activation of microorganisms in rumen, increase the production of propionic acid in rumen, synthesis of microbial protein, and increase useful bacteria in the body It is confirmed by animal experiments etc. that the digestion and absorption efficiency is improved, the digestibility of the feed, in particular, the digestibility of the feed containing solids is improved, and the energy loss is reduced.
  • a utilization efficiency improving agent, a method for improving ruminant feed utilization efficiency, a ruminant breeding method, and a ruminant feed were completed.
  • the gist of the present invention is the ruminant feed utilization efficiency improving agent described in (1) to (9) below and the ruminant feed utilization efficiency improving method described in (10) to (18) below.
  • a feed utilization efficiency improver for ruminants comprising viable cells of Bacillus subtilis as an active ingredient.
  • the feed utilization efficiency improving agent for ruminants according to (1) wherein the feed utilization efficiency improvement is accompanied by an improvement in feed digestibility.
  • feed utilization efficiency improving agent for ruminants according to (1) which is accompanied by at least one increase in useful bacteria in the body.
  • the feed utilization efficiency improving agent for ruminants according to (4) wherein useful bacteria are lactobacilli and bifidobacteria.
  • the gist of the present invention is the ruminant breeding method described in the following (19) to (23) and the ruminant feed described in the following (24) to (28).
  • a ruminant breeding method comprising feeding a ruminant having a ruminant function after the late weaning period with live cells of Bacillus subtilis directly or added to a feed.
  • the present invention can provide an improving agent that has the effect of improving the specific digestion and absorption mechanism and the general energy metabolism mechanism of ruminants.
  • the present invention improves rumen fermentation of ruminants without using antibiotics, special amino acids, etc., promotes the increase and / or activation of microorganisms in rumen, and produces propionic acid in rumen, microorganisms Improve body protein synthesis, further reduce harmful bacteria in the body and increase useful bacteria to improve digestion and absorption efficiency, improve the digestibility of feed, especially the feed containing solids, It is possible to provide a feed utilization efficiency improving agent and a milk yield improving agent for ruminants characterized by reducing energy loss.
  • ruminants which are domestic animals, directly or after being added to feed
  • rumen fermentation of ruminants is improved and synthesis of microbial protein in the rumen is improved, so that the highly toxic ammonia concentration in the rumen fluid
  • digestion and absorption efficiency is improved, and it is possible to nurture livestock with improved health and milk quality.
  • the feed utilization efficiency can be improved, that is, the digestibility of the feed can be improved and energy loss can be reduced, so that the amount of feed fed can be reduced. It is advantageous in that it improves productivity and is economically advantageous, and can improve the quantity and quality of livestock emissions and contribute to environmental improvement.
  • FIG. 6 is a diagram showing a change (decrease) in ammonia nitrogen concentration in the rumen liquid of Test Example 4. It is a figure which shows the change (increase) of the number of protozoa in the rumen liquid of Test Example 5. It is a figure which shows the change (decrease of harmful bacteria and increase of useful bacteria) of various microbes in the feces of Experimental example 6.
  • FIG. 6 is a diagram showing a change (decrease) in ammonia nitrogen concentration in the rumen liquid of Test Example 4. It is a figure which shows the change (increase) of the number of protozoa in the rumen liquid of Test Example 5. It is a figure which shows the change (decrease of harmful bacteria and increase of useful bacteria) of various microbes in the feces of Experimental example 6.
  • the viable cell contained as an essential active ingredient is Bacillus subtilis, and its bacteriological properties are described in Birge's Manual of Bacteriology Vol. 11 (1986).
  • Bacillus subtilis any known Bacillus subtilis can be used, and for example, Bacillus subtilis C-3102 and the like are preferable.
  • a liquid medium or a solid medium containing a carbon source, a nitrogen source, an inorganic substance or the like that is usually used for microbial culture can be used as a medium.
  • the carbon source may be any carbon source that can assimilate Bacillus subtilis, for example, glucose, fructose, sucrose, starch, molasses, etc.
  • the nitrogen source for example, peptone, casein hydrolyzate, meat extract, An ammonium sulfate etc. can be mentioned.
  • salts such as phosphoric acid, potassium, magnesium, calcium, sodium, iron and manganese, vitamins, amino acids, surfactants and the like can be added as necessary.
  • the culture conditions are preferably aerobic conditions, and it is preferable to use, for example, an aeration and agitation liquid culture using a jar fermenter, a shelf-type solid culture, or an automatic koji culture apparatus as the culture apparatus.
  • the culture temperature is preferably 20 to 50 ° C., particularly preferably 30 to 45 ° C.
  • the culture time is preferably 12 hours to 7 days
  • the culture pH is preferably 5 to 9, particularly preferably 6 to 8.
  • the culture itself, the concentrate thereof, or the cells isolated therefrom can be used as active ingredients as they are.
  • the active ingredient contains, for example, 10 to 10 12 cells / g, preferably 10 6 to 10 11 viable cells of Bacillus subtilis C-3102 (FERM BP-1096), that is, spores and / or vegetative cells. It is good to contain pieces / g.
  • the active ingredient can be used as it is as an improving agent.
  • the excipient is not particularly limited, and for example, calcium carbonate, defatted rice sugar, corn grits, corn flour, bran, nonfat dry milk and the like can be used.
  • the administration form of the feed utilization efficiency improving agent for ruminants of the present invention is not particularly limited, and may be given to ruminants as it is, but is preferably added to and mixed with a known feed as a powder, particularly a fine powder. Can be used.
  • the addition amount of the improving agent of the present invention when added to a known feed varies depending on the type of livestock, age, etc., but for example, Bacillus subtilis C-3102 (FERM BP-1096) after being added to the feed It is preferable to add and mix so that the number of viable bacteria is 10 3 to 10 9 cells / g, particularly 10 4 to 10 8 cells / g.
  • the effective intake of the ruminant feed utilization efficiency improving agent of the present invention varies depending on the type of ruminant, age, and the like.
  • the viable count of Bacillus subtilis C-3102 (FERM BP-1096) is 1 It is preferable to ingest at 10 ⁇ 10 6 / Kg ⁇ day or more, particularly 5 ⁇ 10 6 to 1 ⁇ 10 8 / Kg ⁇ day.
  • the administration time varies depending on the type of ruminant, age, etc., but continuous administration is preferred. For example, in the case of cows, it is particularly effective to administer to a weaning period using a solid-containing feed or to a growing cow after weaning.
  • the stomach of the cow is not differentiated and there is a lumen Is not developed.
  • the entrance of the lumen is closed and the lumen does not enter, so that it flows downward, and it is in the same state as monogastric animals like humans and pigs.
  • the entrance to the lumen opens in response.
  • the improving agent of the present invention is used. Giving and improving rumen fermentation and promoting the increase and activation of microorganisms such as protozoa in the rumen can be said to have a great effect on the subsequent growth.
  • the kind of ruminant subject to the present invention is not particularly limited, but preferably, it is a ruminant whose milk is a commercial product, such as cows, sheep, goats, and the like, and is raised as livestock. .
  • the feed utilization efficiency improving agent of the present invention can also be used as a milk yield improving agent, but the target is not limited to dairy cows and the like. The details and effects of the present invention will be described below with reference to examples and test examples, but the present invention is not limited to these examples.
  • a medium is prepared by dissolving 200 g of soybean peptone, 10 g of dipotassium phosphate and 200 g of molasses in 10 liters of tap water, and further adjusting the pH of the medium to 7.5 by adding a 1N aqueous sodium hydroxide solution.
  • the fermenter was charged and sterilized at 121 ° C. for 15 minutes. Subsequently, it was cooled to 37 ° C., 100 ml of a culture solution of Bacillus subtilis C-3102 (FERM BP-1096) that had been pre-cultured in advance was ingested, and aeration and shaking culture was performed at 37 ° C. for 40 hours.
  • the obtained culture solution is centrifuged to collect the cells and dried, and then mixed with 180 g of calcium carbonate to obtain a ruminant feed utilization improving agent (hereinafter referred to as “improving agent 1”). 200 g was produced.
  • the viable cell count of Bacillus subtilis C-3102 (FERM BP-1096) in the improving agent 1 obtained was 1 ⁇ 10 10 cells / g.
  • the samples added with 30 ppm, 60 ppm, and 90 ppm were obtained by adding 3 ⁇ 10 5 cells / g, 6 ⁇ 10 5 cells / g, and 9 ⁇ 10 5 cells / g to the substrate (mixed feed), respectively. Become. A substrate alone (no addition of 1 improver) was used as a control. (Test method): The above culture solution was placed in the bottle of each sample, the gas was replaced, and the anaerobic culture was performed at 39 ° C for 6 hours. After 6 hours, pH, methane gas, hydrogen gas and the like were measured.
  • Volatile fatty acids are an energy source used to assemble milk components in the breast (see Non-Patent Document 1).
  • Propionic acid is the most energy-efficient acid among volatile fatty acids and absorbs from rumen. It is known as a good acid. Therefore, it can be said that the result of Test Example 1 suggests improvement of rumen fermentation by the improving agent of the present invention, improvement of feed utilization efficiency, and improvement of milk yield. Next, the actual cow was raised and the effect was confirmed.
  • Test Example 4 ⁇ Reduction of ammonia nitrogen concentration in rumen solution (improvement of microbial protein synthesis)> The test was conducted under the above test conditions, and each rumen solution was collected on the last day of each of the control period 1, the test period, and the control period 2, and the ammonia nitrogen concentration (mg / dl) in the liquid was measured. Average values for the control period (1, 2) and the test period were obtained. The results are shown in Table 4 and FIG.
  • Test Example 5 ⁇ Increase and / or activation of microorganisms in rumen> The test was conducted under the test conditions described above, and the three test cows were treated with 0 hour before feeding, 2 hours after feeding, and 5 hours after feeding on the last day of each of control period 1, test period, and control period 2. The rumen fluid is collected, the number of protozoa (protozoa) in the fluid is measured, and all the measured values at each time in the control period 1 and 2 and all the measured values at each time in the test period are summed up to obtain an average value. I put it out. The results are shown in Table 5 and FIG.
  • the average value of the summary of the number of protozoa is 3.5 ⁇ 10 5 cells / ml in the control period and 4.3 ⁇ 10 5 cells / ml in the test period, and the average number of protozoa in the test period using the improving agent 1 The value was high.
  • Microorganisms in the rumen are involved in rumen fermentation and decompose proteins, as well as new microbial proteins with high nutritional value (bacterial proteins, parasite proteins, using ammonia, which is a degradation product of the protein source) ), And protozoa, etc., is transported to the 4th and subsequent stomachs together with the stomach contents as microbial protein, and is digested and absorbed in the small intestine.
  • Test Example 6 ⁇ About reduction of harmful bacteria and increase of useful bacteria> Perform the test under the above test conditions, collect fresh feces of each cow in the latter half of each of the control period 1, test period, and control period 2, and culture and measure the flora in the feces using the modified Mitsuoka method. The average value was obtained for each period. The results are shown in Table 6 and FIG. In the table, ND indicates the detection limit (2.30 CFU / g) or less, and (fraction) below the numerical value (average value ⁇ standard deviation) indicates the detection rate.
  • bifidobacteria are below the detection limit (ND) in both the control period 1 and the control period 2 and increase significantly only in the test period.
  • ND detection limit
  • lactobacilli and bifidobacteria maintain a good intestinal environment, improve absorbability, and help maintain health, but these bacteria are not used as feed additives from outside. It is said that there are many problems, such as being troublesome (for example, refer Unexamined-Japanese-Patent No. 2009-45).
  • Test example 7 ⁇ Methane gas generation amount> The test was conducted under the above test conditions, and the methane gas generation amount (L / DMI / day) was measured with a hood-type breathing test apparatus on the last day of each of the control period 1, the test period, and the control period 2, Average values for the control period (1, 2) and the test period were obtained. The results are shown in Table 7.
  • the present invention can provide an improving agent capable of improving the specific digestion and absorption mechanism and the general energy metabolism mechanism of ruminants, it has great industrial applicability.
  • the present invention improves rumen fermentation of ruminants without using antibiotics, special amino acids, etc., promotes the increase and / or activation of rumen microorganisms, and produces propionic acid in the rumen. Characterized by improving protein production, further reducing harmful bacteria in the body and increasing useful bacteria, improving the digestibility of feeds, especially feeds containing solids, and reducing energy loss
  • the feed utilization efficiency improving agent and milk yield improving agent for ruminants can be provided, and thus the industrial applicability is great.
  • the improving agent of the present invention By feeding the improving agent of the present invention directly or by adding it to the feed, rumen fermentation of ruminants is improved and synthesis of bacterial proteins in the rumen is improved. Therefore, highly toxic ammonia in the rumen fluid As the concentration decreases and harmful bacteria in the body decrease and useful bacteria increase, healthy animals with improved milk yield and quality can be bred, productivity is improved, and economic contributions are made. The above applicability is great. Furthermore, by feeding the improving agent of the present invention directly or by adding it to the feed, the feed utilization efficiency can be improved, that is, the digestibility of the feed can be improved and the energy loss can be reduced. It is possible to improve productivity and productivity, as well as improve the quantity and quality of livestock and contribute to environmental improvement.

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Abstract

L'invention porte sur un agent améliorant le rendement d'utilisation d'aliments pour animaux ruminants, tels que vaches, moutons et chèvres, lequel agent améliorant exerce un effet d'aptitude à l'élevage de bétail sain dans lequel la fermentation dans le rumen est améliorée sans utiliser de substances antibiotiques ou d'acides aminés spéciaux, le nombre de bactéries utiles dans le corps étant accru, la digestibilité des aliments étant améliorée, la perte d'énergie étant réduite, et le rendement d'utilisation d'aliments et la production laitière étant améliorées. L'invention porte également sur un procédé pour l'amélioration du rendement d'utilisation d'aliments pour animaux ruminants, sur un procédé pour l'élevage d'animaux ruminants et sur des aliments pour animaux ruminants. De manière spécifique, l'invention porte sur un agent améliorant du rendement d'utilisation d'aliments pour animaux ruminants, sur un procédé pour l'amélioration du rendement d'utilisation d'aliments d'animaux ruminants, sur un procédé pour l'élevage d'animaux ruminants et sur des aliments pour animaux ruminants dans lesquels les cellules vivantes de Bacillus subtilis étant utilisées comme principe actif.
PCT/JP2011/057270 2010-03-17 2011-03-17 Agent améliorant le rendement d'utilisation d'aliments pour animaux ruminants WO2011115306A1 (fr)

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JP2013147469A (ja) * 2012-01-20 2013-08-01 Calpis Co Ltd 腸内酪酸産生菌増加剤
WO2014185516A1 (fr) 2013-05-17 2014-11-20 カルピス株式会社 Agent de prévention ou de traitement de la mastite chez des ruminants
WO2016060935A2 (fr) 2014-10-08 2016-04-21 Novozymes A/S Compositions et procédés d'amélioration de la digestibilité d'aliments pour animaux
CN105724760A (zh) * 2016-02-19 2016-07-06 中国农业科学院饲料研究所 一种农作物秸秆发酵饲料及其制备方法与应用
JP2017226649A (ja) * 2016-06-15 2017-12-28 アサヒグループホールディングス株式会社 骨代謝改善剤
WO2019013382A1 (fr) * 2017-07-12 2019-01-17 씨제이제일제당(주) Additif alimentaire contenant le bacillus subtilis et le bacillus licheniformis, composition d'aliment contenant un additif et procédé de préparation de l'additif alimentaire
WO2019027073A1 (fr) * 2017-08-04 2019-02-07 씨제이제일제당(주) Additif d'aliments pour animaux contenant bacillus subtilis et bacillus licheniformis, composition d'aliments pour animaux contenant ledit additif et procédé de préparation dudit additif
CN109640687A (zh) * 2016-06-29 2019-04-16 株式会社目立康 家畜用饲料效率改善剂、家畜用促增重饲料、家畜的饲养方法以及抑制甲烷产生的方法
CN110169501A (zh) * 2019-05-09 2019-08-27 河北冀丰动物营养科技有限责任公司 一种幼龄反刍动物奶粉
WO2019183511A1 (fr) * 2018-03-23 2019-09-26 Purina Animal Nutrition Llc Procédés d'alimentation de produits d'alimentation animale contenant des agents microbiens en alimentation directe
CN110637919A (zh) * 2019-09-18 2020-01-03 新和县园邦城农业发展有限公司 一种黄粉虫沙、虫蜕的新型秸秆复合饲料
CN114916611A (zh) * 2022-07-20 2022-08-19 山东益得来生物科技有限公司 一种促进育肥羊胃肠道健康的菊芋益生菌剂
WO2023222664A1 (fr) 2022-05-17 2023-11-23 Chr. Hansen A/S Mélange de souches probiotiques pour améliorer la santé et les performances de croissance de ruminants

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JP2013147469A (ja) * 2012-01-20 2013-08-01 Calpis Co Ltd 腸内酪酸産生菌増加剤
US10251918B2 (en) 2013-05-17 2019-04-09 Asahi Calpis Wellness Co., Ltd. Preventive or therapeutic agent for ruminant animal mastitis
CN105358166B (zh) * 2013-05-17 2019-11-12 朝日可尔必思健康株式会社 用于反刍动物的乳腺炎的预防剂或治疗剂
AU2014266266B2 (en) * 2013-05-17 2019-05-16 Asahi Biocycle Co., Ltd. Preventive or therapeutic agent for ruminant animal mastitis
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JPWO2014185516A1 (ja) * 2013-05-17 2017-02-23 アサヒカルピスウェルネス株式会社 反芻動物の乳房炎の予防または治療剤
WO2014185516A1 (fr) 2013-05-17 2014-11-20 カルピス株式会社 Agent de prévention ou de traitement de la mastite chez des ruminants
WO2016060935A2 (fr) 2014-10-08 2016-04-21 Novozymes A/S Compositions et procédés d'amélioration de la digestibilité d'aliments pour animaux
CN105724760A (zh) * 2016-02-19 2016-07-06 中国农业科学院饲料研究所 一种农作物秸秆发酵饲料及其制备方法与应用
JP2017226649A (ja) * 2016-06-15 2017-12-28 アサヒグループホールディングス株式会社 骨代謝改善剤
CN109640687A (zh) * 2016-06-29 2019-04-16 株式会社目立康 家畜用饲料效率改善剂、家畜用促增重饲料、家畜的饲养方法以及抑制甲烷产生的方法
WO2019013382A1 (fr) * 2017-07-12 2019-01-17 씨제이제일제당(주) Additif alimentaire contenant le bacillus subtilis et le bacillus licheniformis, composition d'aliment contenant un additif et procédé de préparation de l'additif alimentaire
KR101934400B1 (ko) * 2017-07-12 2019-03-25 씨제이제일제당 주식회사 바실러스 서브틸리스 및 바실러스 리체니포미스를 포함하는 사료 첨가제, 상기 첨가제를 포함하는 사료 조성물 및 상기 사료첨가제의 제조 방법
JP2019524049A (ja) * 2017-07-12 2019-09-05 シージェイ チェイルジェダン コーポレーションＣｊ Ｃｈｅｉｌｊｅｄａｎｇ Ｃｏｒｐｏｒａｔｉｏｎ バチルスサブチリスとバチルスリケニフォルミスを含む飼料添加剤、前記添加剤を含む飼料組成物及び前記飼料添加剤の製造方法
US11638432B2 (en) 2017-07-12 2023-05-02 Cj Cheiljedang Corporation Feed additive comprising Bacillus subtilis and Bacillus licheniformis, a feed composition comprising the feed additive and a method for producing the feed additive
WO2019027073A1 (fr) * 2017-08-04 2019-02-07 씨제이제일제당(주) Additif d'aliments pour animaux contenant bacillus subtilis et bacillus licheniformis, composition d'aliments pour animaux contenant ledit additif et procédé de préparation dudit additif
WO2019183511A1 (fr) * 2018-03-23 2019-09-26 Purina Animal Nutrition Llc Procédés d'alimentation de produits d'alimentation animale contenant des agents microbiens en alimentation directe
CN110169501A (zh) * 2019-05-09 2019-08-27 河北冀丰动物营养科技有限责任公司 一种幼龄反刍动物奶粉
CN110637919A (zh) * 2019-09-18 2020-01-03 新和县园邦城农业发展有限公司 一种黄粉虫沙、虫蜕的新型秸秆复合饲料
WO2023222664A1 (fr) 2022-05-17 2023-11-23 Chr. Hansen A/S Mélange de souches probiotiques pour améliorer la santé et les performances de croissance de ruminants
CN114916611A (zh) * 2022-07-20 2022-08-19 山东益得来生物科技有限公司 一种促进育肥羊胃肠道健康的菊芋益生菌剂
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