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WO2011107887A3 - Methods for replicating polynucleotides with secondary structure - Google Patents

Methods for replicating polynucleotides with secondary structure Download PDF

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Publication number
WO2011107887A3
WO2011107887A3 PCT/IB2011/000830 IB2011000830W WO2011107887A3 WO 2011107887 A3 WO2011107887 A3 WO 2011107887A3 IB 2011000830 W IB2011000830 W IB 2011000830W WO 2011107887 A3 WO2011107887 A3 WO 2011107887A3
Authority
WO
WIPO (PCT)
Prior art keywords
secondary structure
methods
replicating polynucleotides
replicating
polynucleotides
Prior art date
Application number
PCT/IB2011/000830
Other languages
French (fr)
Other versions
WO2011107887A2 (en
Inventor
Sydney Brenner
Robert Osborne
Andrew Slatter
Original Assignee
Population Genetic Technologies Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US30976810P priority Critical
Priority to US61/309,768 priority
Application filed by Population Genetic Technologies Ltd. filed Critical Population Genetic Technologies Ltd.
Publication of WO2011107887A2 publication Critical patent/WO2011107887A2/en
Publication of WO2011107887A3 publication Critical patent/WO2011107887A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Abstract

Aspects of the present invention are drawn to processes for replicating polynucleotide sequences having secondary structure(s) that reduce or inhibit the replication process. Compositions, kits and systems that find use in carrying out the replication processes described herein are also provided.
PCT/IB2011/000830 2010-03-02 2011-02-28 Methods for replicating polynucleotides with secondary structure WO2011107887A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US30976810P true 2010-03-02 2010-03-02
US61/309,768 2010-03-02

Publications (2)

Publication Number Publication Date
WO2011107887A2 WO2011107887A2 (en) 2011-09-09
WO2011107887A3 true WO2011107887A3 (en) 2012-01-19

Family

ID=44259647

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2011/000830 WO2011107887A2 (en) 2010-03-02 2011-02-28 Methods for replicating polynucleotides with secondary structure

Country Status (1)

Country Link
WO (1) WO2011107887A2 (en)

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202B1 (en) 1985-03-28 1990-11-27 Cetus Corp
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4683195B1 (en) 1986-01-30 1990-11-27 Cetus Corp
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5168038A (en) 1988-06-17 1992-12-01 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP3080178B2 (en) 1991-02-18 2000-08-21 東洋紡績株式会社 Amplification methods and reagent kits for its nucleic acid sequence
US5426180A (en) 1991-03-27 1995-06-20 Research Corporation Technologies, Inc. Methods of making single-stranded circular oligonucleotides
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
CA2160016C (en) 1993-04-12 2008-06-03 Robert L. Letsinger Method of forming oligonucleotides
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
SE9400522D0 (en) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
ES2326050T5 (en) 1996-06-04 2012-04-26 University Of Utah Research Foundation Monitoring hybridization during PCR
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
WO2004029223A2 (en) 2002-09-30 2004-04-08 Parallele Bioscience, Inc. Polynucleotide synthesis and labeling by kinetic sampling ligation
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007087310A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CARTER P ET AL: "Improved oligonucleotide site-directed mutagenesis using M13 vectors.", NUCLEIC ACIDS RESEARCH 25 JUN 1985 LNKD- PUBMED:2989795, vol. 13, no. 12, 25 June 1985 (1985-06-25) , pages 4431-4443, XP002650917, ISSN: 0305-1048 *
FRATCZAK AGATA ET AL: "LNA-modified primers drastically improve hybridization to target RNA and reverse transcription.", BIOCHEMISTRY 27 JAN 2009 LNKD- PUBMED:19119855, vol. 48, no. 3, 27 January 2009 (2009-01-27), pages 514-516, XP002650915, ISSN: 1520-4995 *
STEWART J ET AL: "A quantitative assay for assessing allelic proportions by iterative gap ligation.", NUCLEIC ACIDS RESEARCH 15 FEB 1998 LNKD- PUBMED:9461454, vol. 26, no. 4, 15 February 1998 (1998-02-15), pages 961-966, XP002650916, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2011107887A2 (en) 2011-09-09

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