WO2011106576A3 - Methods and compositions for enhanced enzyme activity in fermenting microorganisms - Google Patents

Methods and compositions for enhanced enzyme activity in fermenting microorganisms Download PDF

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Publication number
WO2011106576A3
WO2011106576A3 PCT/US2011/026143 US2011026143W WO2011106576A3 WO 2011106576 A3 WO2011106576 A3 WO 2011106576A3 US 2011026143 W US2011026143 W US 2011026143W WO 2011106576 A3 WO2011106576 A3 WO 2011106576A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
enzyme activity
fermenting microorganisms
enhanced enzyme
Prior art date
Application number
PCT/US2011/026143
Other languages
French (fr)
Other versions
WO2011106576A2 (en
Inventor
Matthias Schmalisch
William G. Latouf
Patrick O'mullan
Muhammad Abdul Ali
Original Assignee
Qteros, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qteros, Inc. filed Critical Qteros, Inc.
Publication of WO2011106576A2 publication Critical patent/WO2011106576A2/en
Publication of WO2011106576A3 publication Critical patent/WO2011106576A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

This invention provides systems and methods for the production of compounds by recombinant microorganism that express species whereby the recombinant species are genetically-modified, for hydrolysis and fermentation of carbonaceous biomass to synthesize compounds of commercial value. Genetic modification can be for the expression of low temperature-adapted enzymes. The recombinant microorganism can be a Clostridium, such as C. phytofermentans.
PCT/US2011/026143 2010-02-24 2011-02-24 Methods and compositions for enhanced enzyme activity in fermenting microorganisms WO2011106576A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US30768110P 2010-02-24 2010-02-24
US61/307,681 2010-02-24
US201161442120P 2011-02-11 2011-02-11
US61/442,120 2011-02-11

Publications (2)

Publication Number Publication Date
WO2011106576A2 WO2011106576A2 (en) 2011-09-01
WO2011106576A3 true WO2011106576A3 (en) 2012-01-12

Family

ID=44507570

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/026143 WO2011106576A2 (en) 2010-02-24 2011-02-24 Methods and compositions for enhanced enzyme activity in fermenting microorganisms

Country Status (1)

Country Link
WO (1) WO2011106576A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012103385A2 (en) * 2011-01-26 2012-08-02 Qteros, Inc. Biocatalysts synthesizing deregulated cellulases
CN104087622B (en) * 2014-07-04 2016-09-14 哈尔滨工业大学 The method utilizing lignocellulose biomass co-producing butanol and hydrogen
CN110241139A (en) * 2019-05-14 2019-09-17 西安建筑科技大学 Ice-melting agent stock solution, method and application for preparing ice-melting agent stock solution by using straw powder
CN110511974A (en) * 2019-09-18 2019-11-29 宁夏泰益欣生物科技有限公司 A kind of fermentation process improving abomacetin fermentation potency
CN112342157A (en) * 2020-10-26 2021-02-09 西南大学 A kind of separation and preparation method of cow dung microbial fermentation strain
CN113321563A (en) * 2021-06-18 2021-08-31 创想未来生物工程(北京)有限公司 Seaweed organic water-soluble fertilizer

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008098227A2 (en) * 2007-02-09 2008-08-14 The Regents Of The University Of California Biofuel production by recombinant microorganisms
US20100035320A1 (en) * 2008-07-28 2010-02-11 Jeffrey Blanchard Methods and compositions for improving the production of products in microorganisms
US20100268000A1 (en) * 2009-04-20 2010-10-21 Qteros, Inc. Compositions and Methods for Fermentation of Biomass

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008098227A2 (en) * 2007-02-09 2008-08-14 The Regents Of The University Of California Biofuel production by recombinant microorganisms
US20100035320A1 (en) * 2008-07-28 2010-02-11 Jeffrey Blanchard Methods and compositions for improving the production of products in microorganisms
US20100268000A1 (en) * 2009-04-20 2010-10-21 Qteros, Inc. Compositions and Methods for Fermentation of Biomass

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GARSOUX, G. ET AL.: "Kinetic and structural optimization to catalysis at low temperatures in a psychrophilic cellulase from the antarctic bacterium Pseudoalteromonas haloplanktis", BIOCHEMICAL JOURNAL, vol. 384, no. PT.2, 1 December 2004 (2004-12-01), pages 247 - 253 *
GUO, B. ET AL.: "Gene cloning, expression and characterization of a new cold-active and salt-tolerant endo-beta-1,4-xylanase from marine Glaciecola mesophila KMM241", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY., vol. 84, no. 6, 9 June 2009 (2009-06-09), pages 1107 - 1115, XP019757449, DOI: doi:10.1007/s00253-009-2056-y *

Also Published As

Publication number Publication date
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