WO2011090804A1 - Targeted genomic alteration - Google Patents
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- WO2011090804A1 WO2011090804A1 PCT/US2011/000125 US2011000125W WO2011090804A1 WO 2011090804 A1 WO2011090804 A1 WO 2011090804A1 US 2011000125 W US2011000125 W US 2011000125W WO 2011090804 A1 WO2011090804 A1 WO 2011090804A1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
- C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Definitions
- Such traits in plants include, but are not limited to, herbicide resistance or tolerance; insect resistance or tolerance; disease resistance or tolerance (viral, bacterial, fungal, nematode); stress tolerance and/or resistance, as exemplified by resistance or tolerance to drought, heat, chilling, freezing, excessive moisture, salt stress; oxidative stress; increased yields; food content and makeup; physical appearance; male sterility; drydown; standability; prolificacy; starch quantity and quality; oil quantity and quality; protein quality and quantity; amino acid composition; and the like.
- Figure 7 is a plasmid map of pDAB105900.
- binding interaction components of a binding interaction need be sequence-specific (e.g., contacts with phosphate residues in a DNA backbone), as long as the interaction as a whole is sequence-specific. Such interactions are generally characterized by a dissociation constant (3 ⁇ 4) of 10 "6 M “1 or lower. "Affinity" refers to the strength of binding:
- Restriction endonucleases are present in many species and are capable of sequence-specific binding to DNA (at a recognition site), and cleaving DNA at or near the site of binding.
- Certain restriction enzymes e.g., Type IIS
- the Type IIS enzyme Fokl catalyzes double-stranded cleavage of DNA, at 9 nucleotides from its recognition site on one strand and 13 nucleotides from its recognition site on the other. See, for example, US Patents 5,356,802; 5,436,150 and 5,487,994; as well as Li et al.
- polynucleotides encoding the fusion proteins are constructed. These polynucleotides can be inserted into a vector and the vector can be introduced into a cell (see below for additional disclosure regarding vectors and methods for introducing polynucleotides into cells).
- ZFV-Fokl fusions are arranged such that the cleavage half-domain is nearest the amino terminus of the fusion protein, and the zinc finger domain is nearest the carboxy-terminus.
- dimerization of the cleavage half-domains to form a functional nuclease is brought about by binding of the fusion proteins to sites on opposite DNA strands, with the 3' ends of the binding sites being proximal to each other.
- nucleic acids encoding engineered ZFPs can be used to introduce nucleic acids encoding engineered ZFPs into mammalian cells. Such methods can also be used to administer nucleic acids encoding ZFPs to mammalian cells in vitro. In certain embodiments, nucleic acids encoding ZFPs are administered for in vivo or ex vivo uses.
- Embryogenic Hi-II cell cultures of maize were produced, and were used as the source of living plant cells in which integration was demonstrated.
- One skilled in the art may envision the utilization of cell cultures derived from a variety of plant species, or differentiated plant tissues derived from a variety of plant species, as the source of living plant cells in which integration was demonstrated.
- Genomic DNA was extracted from isolated maize cells as described in Example 2.2 and utilized as template for PCR genotyping experiments. gDNA was extracted from approximately 100-300 ⁇ packed cell volume (PCV) of Hi-II callus that were isolated as described above according to the manufacturer's protocols detailed in the DNeasy 96 Plant Kit (QIAGEN Inc., Valencia, CA).
- TAQMAN ® assays were performed to screen samples of herbicide resistant callus to identify those that contained single copy integration of the pDAB 105908 transgene. Detailed analysis was conducted using primers and probes specific to gene expression cassettes. Single copy events were identified for additional analysis.
- Custom TAQMAN ® assays were developed for PAT gene analysis in
- Integrity of the DNA was estimated by running all samples on 0.8% agarose E-gels (Invitrogen, Carlsbad, CA). All samples were normalized (25 ng/ul) for PCR amplification to generate amplicons for Solexa sequencing.
- LIGHTCYCLER ® 480 Probes Master mix was prepared at IX final concentration in a 10 volume multiplex reaction containing 0.4 ⁇ of each primer and 0.2 ⁇ of each probe (Table 4 below).
- a two step amplification reaction is performed with an extension at 58°C for 38 seconds with fluorescence acquisition. All samples were run in triplicate and the averaged Ct values were used for analysis of each sample.
- Analysis of real time PCR data was performed using LIGHTCYCLER ® software using the relative quant module and was based on the AACt method. For this, a sample of gDNA from a single copy calibrator was included to normalize results. The single copy calibrator event was identified by Southern analysis and was confirmed to have a single insert of the PAT gene.
- Table 4 Primers and probes used in PAT and PAL hydrolysis probe assays
- pDAB1601 negative control - PAT only
- pDAB4346 positive control - ZFN-ILl only
- pDAB4346 + pDABeZFN-X ZFN-ILl + eZFN to be tested
- Genomic DNA was isolated from Agrobacterium-treated, transgenic tobacco leaf discs using a Qiagen DNA extraction kit. All treatments were in duplicate and genomic DNA from all samples was re-suspended in 100 of water and concentrations were determined by the Nanodrop. Equal amounts of genomic DNA from each replicate for individual treatments was pooled together and was used as a starting template for Solexa amplicon generation.
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
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Abstract
Description
Claims
Priority Applications (12)
Application Number | Priority Date | Filing Date | Title |
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JP2012550018A JP5902631B2 (en) | 2010-01-22 | 2011-01-24 | Targeted genome modification |
KR1020127021851A KR101866578B1 (en) | 2010-01-22 | 2011-01-24 | Targeted genomic alteration |
MX2012008490A MX336846B (en) | 2010-01-22 | 2011-01-24 | Targeted genomic alteration. |
CA2787494A CA2787494C (en) | 2010-01-22 | 2011-01-24 | Targeted genomic alteration |
BR112012018249A BR112012018249A2 (en) | 2010-01-22 | 2011-01-24 | target genomic change |
UAA201210055A UA118328C2 (en) | 2010-01-22 | 2011-01-24 | Targeted gemomic alteration |
AU2011207769A AU2011207769B2 (en) | 2010-01-22 | 2011-01-24 | Targeted genomic alteration |
CN201180013070.5A CN102812034B (en) | 2010-01-22 | 2011-01-24 | Target gene group is transformed |
NZ601247A NZ601247A (en) | 2010-01-22 | 2011-01-24 | Targeted genomic alteration |
EA201201036A EA031322B1 (en) | 2010-01-22 | 2011-01-24 | Cell or cell line for expression of exogenous nucleic acid sequences and use of a cell or cell line |
EP11734969.6A EP2526112B1 (en) | 2010-01-22 | 2011-01-24 | Targeted genomic alteration |
IL22102912A IL221029B (en) | 2010-01-22 | 2012-07-19 | Targeted genomic alteration |
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US33645710P | 2010-01-22 | 2010-01-22 | |
US61/336,457 | 2010-01-22 |
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PCT/US2011/000125 WO2011090804A1 (en) | 2010-01-22 | 2011-01-24 | Targeted genomic alteration |
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US (1) | US10260062B2 (en) |
EP (1) | EP2526112B1 (en) |
JP (1) | JP5902631B2 (en) |
KR (1) | KR101866578B1 (en) |
CN (2) | CN106086047B (en) |
AR (1) | AR080009A1 (en) |
AU (1) | AU2011207769B2 (en) |
BR (1) | BR112012018249A2 (en) |
CA (1) | CA2787494C (en) |
CL (1) | CL2012002033A1 (en) |
CO (1) | CO6561839A2 (en) |
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Citations (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4186183A (en) | 1978-03-29 | 1980-01-29 | The United States Of America As Represented By The Secretary Of The Army | Liposome carriers in chemotherapy of leishmaniasis |
US4217344A (en) | 1976-06-23 | 1980-08-12 | L'oreal | Compositions containing aqueous dispersions of lipid spheres |
US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
US4261975A (en) | 1979-09-19 | 1981-04-14 | Merck & Co., Inc. | Viral liposome particle |
US4485054A (en) | 1982-10-04 | 1984-11-27 | Lipoderm Pharmaceuticals Limited | Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV) |
US4501728A (en) | 1983-01-06 | 1985-02-26 | Technology Unlimited, Inc. | Masking of liposomes from RES recognition |
US4774085A (en) | 1985-07-09 | 1988-09-27 | 501 Board of Regents, Univ. of Texas | Pharmaceutical administration systems containing a mixture of immunomodulators |
US4837028A (en) | 1986-12-24 | 1989-06-06 | Liposome Technology, Inc. | Liposomes with enhanced circulation time |
US4897355A (en) | 1985-01-07 | 1990-01-30 | Syntex (U.S.A.) Inc. | N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4946787A (en) | 1985-01-07 | 1990-08-07 | Syntex (U.S.A.) Inc. | N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US5049386A (en) | 1985-01-07 | 1991-09-17 | Syntex (U.S.A.) Inc. | N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
WO1991016024A1 (en) | 1990-04-19 | 1991-10-31 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
WO1991017424A1 (en) | 1990-05-03 | 1991-11-14 | Vical, Inc. | Intracellular delivery of biologically active substances by means of self-assembling lipid complexes |
US6008336A (en) | 1994-03-23 | 1999-12-28 | Case Western Reserve University | Compacted nucleic acids and their delivery to cells |
US6453242B1 (en) | 1999-01-12 | 2002-09-17 | Sangamo Biosciences, Inc. | Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites |
US6503717B2 (en) | 1999-12-06 | 2003-01-07 | Sangamo Biosciences, Inc. | Methods of using randomized libraries of zinc finger proteins for the identification of gene function |
US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US6599692B1 (en) | 1999-09-14 | 2003-07-29 | Sangamo Bioscience, Inc. | Functional genomics using zinc finger proteins |
US20030232410A1 (en) | 2002-03-21 | 2003-12-18 | Monika Liljedahl | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
US6689558B2 (en) | 2000-02-08 | 2004-02-10 | Sangamo Biosciences, Inc. | Cells for drug discovery |
US20050026157A1 (en) | 2002-09-05 | 2005-02-03 | David Baltimore | Use of chimeric nucleases to stimulate gene targeting |
US20050064474A1 (en) | 2003-08-08 | 2005-03-24 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US20050208489A1 (en) | 2002-01-23 | 2005-09-22 | Dana Carroll | Targeted chromosomal mutagenasis using zinc finger nucleases |
US7013219B2 (en) | 1999-01-12 | 2006-03-14 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US20060188987A1 (en) | 2003-08-08 | 2006-08-24 | Dmitry Guschin | Targeted deletion of cellular DNA sequences |
US20060195937A1 (en) | 1997-11-18 | 2006-08-31 | Pioneer Hi-Bred International, Inc. | Compositions and methods for genetic modification of plants |
WO2007014275A2 (en) | 2005-07-26 | 2007-02-01 | Sangamo Biosciences, Inc. | Targeted integration and expression of exogenous nucleic acid sequences |
WO2007139898A2 (en) | 2006-05-25 | 2007-12-06 | Sangamo Biosciences, Inc. | Variant foki cleavage half-domains |
WO2008021207A2 (en) | 2006-08-11 | 2008-02-21 | Dow Agrosciences Llc | Zinc finger nuclease-mediated homologous recombination |
US20080182332A1 (en) | 2006-12-14 | 2008-07-31 | Cai Qihua C | Optimized non-canonical zinc finger proteins |
US20090205083A1 (en) | 2007-09-27 | 2009-08-13 | Manju Gupta | Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes |
WO2010077319A1 (en) | 2008-12-17 | 2010-07-08 | Dow Agrosciences Llc | Targeted integration into the zp15 locus |
Family Cites Families (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5140081A (en) * | 1989-03-21 | 1992-08-18 | Ciba-Geigy Corporation | Peroxide free radical initiators containing hindered amine moieties with low basicity |
SE466987B (en) * | 1990-10-18 | 1992-05-11 | Stiftelsen Ct Foer Dentaltekni | DEVICE FOR DEEP-SELECTIVE NON-INVASIVE, LOCAL SEATING OF ELECTRICAL IMPEDANCE IN ORGANIC AND BIOLOGICAL MATERIALS AND PROBE FOR SEATING ELECTRICAL IMPEDANCE |
US5487994A (en) | 1992-04-03 | 1996-01-30 | The Johns Hopkins University | Insertion and deletion mutants of FokI restriction endonuclease |
US5436150A (en) | 1992-04-03 | 1995-07-25 | The Johns Hopkins University | Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease |
US5356802A (en) | 1992-04-03 | 1994-10-18 | The Johns Hopkins University | Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease |
CA2681922C (en) | 1994-01-18 | 2012-05-15 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
US6242568B1 (en) | 1994-01-18 | 2001-06-05 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
US6140466A (en) | 1994-01-18 | 2000-10-31 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
ATE407205T1 (en) | 1994-08-20 | 2008-09-15 | Gendaq Ltd | IMPROVEMENT IN BINDING PROTEINS IN DNA DETECTION |
GB9824544D0 (en) | 1998-11-09 | 1999-01-06 | Medical Res Council | Screening system |
US5789538A (en) | 1995-02-03 | 1998-08-04 | Massachusetts Institute Of Technology | Zinc finger proteins with high affinity new DNA binding specificities |
US5925523A (en) | 1996-08-23 | 1999-07-20 | President & Fellows Of Harvard College | Intraction trap assay, reagents and uses thereof |
GB2338237B (en) | 1997-02-18 | 2001-02-28 | Actinova Ltd | In vitro peptide or protein expression library |
GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
GB9710809D0 (en) | 1997-05-23 | 1997-07-23 | Medical Res Council | Nucleic acid binding proteins |
GB9710807D0 (en) | 1997-05-23 | 1997-07-23 | Medical Res Council | Nucleic acid binding proteins |
US6410248B1 (en) | 1998-01-30 | 2002-06-25 | Massachusetts Institute Of Technology | General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites |
WO1999045132A1 (en) | 1998-03-02 | 1999-09-10 | Massachusetts Institute Of Technology | Poly zinc finger proteins with improved linkers |
US6140081A (en) | 1998-10-16 | 2000-10-31 | The Scripps Research Institute | Zinc finger binding domains for GNN |
US6794136B1 (en) | 2000-11-20 | 2004-09-21 | Sangamo Biosciences, Inc. | Iterative optimization in the design of binding proteins |
US6435261B1 (en) * | 1999-12-30 | 2002-08-20 | Wayne D. Ailiff | Mechanism for inverting the cope of a molding flask |
AU2001226935B2 (en) | 2000-01-24 | 2006-06-22 | Gendaq Limited | Nucleic acid binding polypeptides characterized by flexible linkers connected nucleic acid binding modules |
US20020061512A1 (en) | 2000-02-18 | 2002-05-23 | Kim Jin-Soo | Zinc finger domains and methods of identifying same |
WO2001088197A2 (en) | 2000-05-16 | 2001-11-22 | Massachusetts Institute Of Technology | Methods and compositions for interaction trap assays |
JP2002060786A (en) | 2000-08-23 | 2002-02-26 | Kao Corp | Germicidal stainproofing agent for hard surface |
GB0108491D0 (en) | 2001-04-04 | 2001-05-23 | Gendaq Ltd | Engineering zinc fingers |
DE10131786A1 (en) | 2001-07-04 | 2003-01-16 | Sungene Gmbh & Co Kgaa | Recombination systems and methods for removing nucleic acid sequences from the genome of eukaryotic organisms |
EP1421177A4 (en) | 2001-08-20 | 2006-06-07 | Scripps Research Inst | Zinc finger binding domains for cnn |
AU2004263865B2 (en) * | 2003-08-08 | 2007-05-17 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
PL1740039T3 (en) | 2004-04-30 | 2013-03-29 | Dow Agrosciences Llc | Novel herbicide resistance genes |
US20100055793A1 (en) | 2005-07-25 | 2010-03-04 | Johns Hopkins University | Site-specific modification of the human genome using custom-designed zinc finger nucleases |
JP5551432B2 (en) | 2006-05-25 | 2014-07-16 | サンガモ バイオサイエンシーズ, インコーポレイテッド | Methods and compositions for gene inactivation |
CA2667414C (en) * | 2006-11-13 | 2015-12-29 | Sangamo Biosciences, Inc. | Methods and compositions for modification of the human glucocorticoid receptor locus |
ATE489465T1 (en) | 2007-04-26 | 2010-12-15 | Sangamo Biosciences Inc | TARGETED INTEGRATION INTO THE PPP1R12C POSITION |
EP2188384B1 (en) * | 2007-09-27 | 2015-07-15 | Sangamo BioSciences, Inc. | Rapid in vivo identification of biologically active nucleases |
BRPI0817911B8 (en) | 2007-10-05 | 2022-06-28 | Dow Agrosciences Llc | PROCESSES FOR TRANSFER OF MOLECULAR SUBSTANCES INTO PLANT CELLS AND PROCESS FOR EXPRESSION OF A GENE |
CA2703045C (en) | 2007-10-25 | 2017-02-14 | Sangamo Biosciences, Inc. | Methods and compositions for targeted integration |
AU2009238629C1 (en) | 2008-04-14 | 2015-04-30 | Sangamo Therapeutics, Inc. | Linear donor constructs for targeted integration |
KR101951489B1 (en) | 2010-01-22 | 2019-04-22 | 다우 아그로사이언시즈 엘엘씨 | Excision of transgenes in genetically modified organisms |
AU2011207769B2 (en) | 2010-01-22 | 2015-05-28 | Corteva Agriscience Llc | Targeted genomic alteration |
-
2011
- 2011-01-24 AU AU2011207769A patent/AU2011207769B2/en active Active
- 2011-01-24 KR KR1020127021851A patent/KR101866578B1/en active IP Right Grant
- 2011-01-24 EP EP11734969.6A patent/EP2526112B1/en active Active
- 2011-01-24 CA CA2787494A patent/CA2787494C/en active Active
- 2011-01-24 BR BR112012018249A patent/BR112012018249A2/en not_active Application Discontinuation
- 2011-01-24 CN CN201610457527.6A patent/CN106086047B/en active Active
- 2011-01-24 EA EA201201036A patent/EA031322B1/en not_active IP Right Cessation
- 2011-01-24 GE GEAP201112838A patent/GEP20176628B/en unknown
- 2011-01-24 NZ NZ601247A patent/NZ601247A/en unknown
- 2011-01-24 JP JP2012550018A patent/JP5902631B2/en active Active
- 2011-01-24 US US12/931,096 patent/US10260062B2/en active Active
- 2011-01-24 UA UAA201210055A patent/UA118328C2/en unknown
- 2011-01-24 CN CN201180013070.5A patent/CN102812034B/en active Active
- 2011-01-24 WO PCT/US2011/000125 patent/WO2011090804A1/en active Application Filing
- 2011-01-24 MX MX2012008490A patent/MX336846B/en active IP Right Grant
- 2011-01-25 AR ARP110100227A patent/AR080009A1/en active IP Right Grant
-
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- 2012-07-19 IL IL22102912A patent/IL221029B/en active IP Right Grant
- 2012-07-20 CL CL2012002033A patent/CL2012002033A1/en unknown
- 2012-08-22 CO CO12141866A patent/CO6561839A2/en not_active Application Discontinuation
Patent Citations (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4217344A (en) | 1976-06-23 | 1980-08-12 | L'oreal | Compositions containing aqueous dispersions of lipid spheres |
US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
US4186183A (en) | 1978-03-29 | 1980-01-29 | The United States Of America As Represented By The Secretary Of The Army | Liposome carriers in chemotherapy of leishmaniasis |
US4261975A (en) | 1979-09-19 | 1981-04-14 | Merck & Co., Inc. | Viral liposome particle |
US4485054A (en) | 1982-10-04 | 1984-11-27 | Lipoderm Pharmaceuticals Limited | Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV) |
US4501728A (en) | 1983-01-06 | 1985-02-26 | Technology Unlimited, Inc. | Masking of liposomes from RES recognition |
US4946787A (en) | 1985-01-07 | 1990-08-07 | Syntex (U.S.A.) Inc. | N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4897355A (en) | 1985-01-07 | 1990-01-30 | Syntex (U.S.A.) Inc. | N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US5049386A (en) | 1985-01-07 | 1991-09-17 | Syntex (U.S.A.) Inc. | N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4774085A (en) | 1985-07-09 | 1988-09-27 | 501 Board of Regents, Univ. of Texas | Pharmaceutical administration systems containing a mixture of immunomodulators |
US4837028A (en) | 1986-12-24 | 1989-06-06 | Liposome Technology, Inc. | Liposomes with enhanced circulation time |
WO1991016024A1 (en) | 1990-04-19 | 1991-10-31 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
WO1991017424A1 (en) | 1990-05-03 | 1991-11-14 | Vical, Inc. | Intracellular delivery of biologically active substances by means of self-assembling lipid complexes |
US6008336A (en) | 1994-03-23 | 1999-12-28 | Case Western Reserve University | Compacted nucleic acids and their delivery to cells |
US20060195937A1 (en) | 1997-11-18 | 2006-08-31 | Pioneer Hi-Bred International, Inc. | Compositions and methods for genetic modification of plants |
US6607882B1 (en) | 1999-01-12 | 2003-08-19 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US6979539B2 (en) | 1999-01-12 | 2005-12-27 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US7163824B2 (en) | 1999-01-12 | 2007-01-16 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US6453242B1 (en) | 1999-01-12 | 2002-09-17 | Sangamo Biosciences, Inc. | Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites |
US7013219B2 (en) | 1999-01-12 | 2006-03-14 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US6824978B1 (en) | 1999-01-12 | 2004-11-30 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US6933113B2 (en) | 1999-01-12 | 2005-08-23 | Sangamo Biosciences, Inc. | Modulation of endogenous gene expression in cells |
US6599692B1 (en) | 1999-09-14 | 2003-07-29 | Sangamo Bioscience, Inc. | Functional genomics using zinc finger proteins |
US6503717B2 (en) | 1999-12-06 | 2003-01-07 | Sangamo Biosciences, Inc. | Methods of using randomized libraries of zinc finger proteins for the identification of gene function |
US6689558B2 (en) | 2000-02-08 | 2004-02-10 | Sangamo Biosciences, Inc. | Cells for drug discovery |
US20050208489A1 (en) | 2002-01-23 | 2005-09-22 | Dana Carroll | Targeted chromosomal mutagenasis using zinc finger nucleases |
US20030232410A1 (en) | 2002-03-21 | 2003-12-18 | Monika Liljedahl | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
US20050026157A1 (en) | 2002-09-05 | 2005-02-03 | David Baltimore | Use of chimeric nucleases to stimulate gene targeting |
US20050064474A1 (en) | 2003-08-08 | 2005-03-24 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US20060188987A1 (en) | 2003-08-08 | 2006-08-24 | Dmitry Guschin | Targeted deletion of cellular DNA sequences |
WO2007014275A2 (en) | 2005-07-26 | 2007-02-01 | Sangamo Biosciences, Inc. | Targeted integration and expression of exogenous nucleic acid sequences |
US20070134796A1 (en) | 2005-07-26 | 2007-06-14 | Sangamo Biosciences, Inc. | Targeted integration and expression of exogenous nucleic acid sequences |
WO2007139898A2 (en) | 2006-05-25 | 2007-12-06 | Sangamo Biosciences, Inc. | Variant foki cleavage half-domains |
US20080131962A1 (en) | 2006-05-25 | 2008-06-05 | Sangamo Biosciences, Inc. | Engineered cleavage half-domains |
WO2008021207A2 (en) | 2006-08-11 | 2008-02-21 | Dow Agrosciences Llc | Zinc finger nuclease-mediated homologous recombination |
US20080182332A1 (en) | 2006-12-14 | 2008-07-31 | Cai Qihua C | Optimized non-canonical zinc finger proteins |
US20090205083A1 (en) | 2007-09-27 | 2009-08-13 | Manju Gupta | Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes |
WO2010077319A1 (en) | 2008-12-17 | 2010-07-08 | Dow Agrosciences Llc | Targeted integration into the zp15 locus |
Non-Patent Citations (22)
Title |
---|
AHMAD ET AL., CANCER RES., vol. 52, 1992, pages 4817 - 4820 |
BEHR ET AL., BIOCONJUGATE CHEM., vol. 5, 1994, pages 382 - 389 |
BLAESE ET AL., CANCER GENE THER, vol. 2, 1995, pages 291 - 297 |
CAI ET AL., PLANT MOL BIOL, vol. 69, 2009, pages 699 - 709 |
CLARK-CURTISS; CURTISS ET AL.: "Methods in Enzymology", vol. 101, 1983, pages: 347 - 362 |
CRYSTAL, SCIENCE, vol. 270, 1995, pages 404 - 410 |
D'HALLUIN ET AL., PLANT BIOTECHNOLOGY J., vol. 6, no. 1, 2008, pages 93 |
DOYON ET AL., NATURE METHODS, vol. 8, no. 1, 2011, pages 74 - 79 |
DURAI ET AL.: "Zinc finder nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells.", NAR, vol. 33, no. 18, 2005, pages 5978 - 5990 * |
FRALEY ET AL., PROC. NAT'L. ACAD. SCI. USA, vol. 80, 1983, pages 4803 |
GAO ET AL., GENE THERAPY, vol. 2, 1995, pages 710 - 722 |
GRIERSON; COREY: "Plant Molecular Biology", 1988, BLACKIE |
HAMMERSCHMIDT ET AL., METHODS CELL BIOL, vol. 59, 1999, pages 87 - 115 |
HORSCH ET AL., SCIENCE, vol. 233, 1984, pages 496 - 498 |
KLEIN ET AL., NATURE, vol. 327, 1987, pages 70 - 73 |
MILLER ET AL., NAT. BIOTECHNOL., vol. 25, no. 7, 2007, pages 778 - 785 |
MOEHLE ET AL., PROC. NATL. ACAD, SCI. USA, vol. 104, no. 9, 2007, pages 3055 - 3060 |
REMY ET AL., BIOCONJUGATE CHEM., vol. 5, 1994, pages 647 - 654 |
See also references of EP2526112A4 |
TERADA ET AL., NAT BIOTECHNOL, vol. 20, no. 10, 2002, pages 1030 |
TERADA ET AL., PLANT PHYSIOL, vol. 144, no. 2, 2007, pages 846 |
WEISSBACH: "Methods for Plant Molecular Biology", 1988, ACADEMIC PRESS, pages: 421 - 463 |
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EA201201036A1 (en) | 2013-01-30 |
CN102812034B (en) | 2016-08-03 |
US20110189775A1 (en) | 2011-08-04 |
AU2011207769B2 (en) | 2015-05-28 |
MX336846B (en) | 2016-02-03 |
MX2012008490A (en) | 2013-04-05 |
JP5902631B2 (en) | 2016-04-13 |
NZ601247A (en) | 2014-10-31 |
EP2526112B1 (en) | 2018-10-17 |
BR112012018249A2 (en) | 2020-02-04 |
IL221029B (en) | 2019-10-31 |
US10260062B2 (en) | 2019-04-16 |
IL221029A0 (en) | 2012-09-24 |
AR080009A1 (en) | 2012-03-07 |
JP2013517770A (en) | 2013-05-20 |
CN106086047B (en) | 2021-07-06 |
CA2787494A1 (en) | 2011-07-28 |
CO6561839A2 (en) | 2012-11-15 |
GEP20176628B (en) | 2017-02-27 |
CN102812034A (en) | 2012-12-05 |
CL2012002033A1 (en) | 2012-12-14 |
AU2011207769A1 (en) | 2012-08-02 |
EP2526112A1 (en) | 2012-11-28 |
CN106086047A (en) | 2016-11-09 |
UA118328C2 (en) | 2019-01-10 |
EP2526112A4 (en) | 2013-12-04 |
EA031322B1 (en) | 2018-12-28 |
KR101866578B1 (en) | 2018-06-11 |
CA2787494C (en) | 2019-09-17 |
KR20120117890A (en) | 2012-10-24 |
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