WO2011090529A1 - Methods and kits for isolating nucleic acid from an organism - Google Patents
Methods and kits for isolating nucleic acid from an organism Download PDFInfo
- Publication number
- WO2011090529A1 WO2011090529A1 PCT/US2010/055123 US2010055123W WO2011090529A1 WO 2011090529 A1 WO2011090529 A1 WO 2011090529A1 US 2010055123 W US2010055123 W US 2010055123W WO 2011090529 A1 WO2011090529 A1 WO 2011090529A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- organism
- agent
- introducing
- sample
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2787639A CA2787639A1 (en) | 2010-01-19 | 2010-11-02 | Methods and kits for isolating nucleic acid from an organism |
EP10844146.0A EP2526186A4 (en) | 2010-01-19 | 2010-11-02 | Methods and kits for isolating nucleic acid from an organism |
AU2010343249A AU2010343249A1 (en) | 2010-01-19 | 2010-11-02 | Methods and kits for isolating nucleic acid from an organism |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/689,270 | 2010-01-19 | ||
US12/689,270 US8932809B2 (en) | 2010-01-19 | 2010-01-19 | Methods and kits for isolating nucleic acid from an organism |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2011090529A1 true WO2011090529A1 (en) | 2011-07-28 |
Family
ID=44277847
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2010/055123 WO2011090529A1 (en) | 2010-01-19 | 2010-11-02 | Methods and kits for isolating nucleic acid from an organism |
Country Status (5)
Country | Link |
---|---|
US (2) | US8932809B2 (en) |
EP (1) | EP2526186A4 (en) |
AU (1) | AU2010343249A1 (en) |
CA (1) | CA2787639A1 (en) |
WO (1) | WO2011090529A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8932809B2 (en) * | 2010-01-19 | 2015-01-13 | Opgen, Inc. | Methods and kits for isolating nucleic acid from an organism |
PL394619A1 (en) * | 2011-04-19 | 2012-10-22 | Miedzynarodowy Instytut Biologii Molekularnej I Komórkowej | Method of proteolysis, peptidase, a composition for use as a bactericide or bacteriostatic agent, a kit and the use of an active form of S. aureus LytM or a derivative thereof |
Citations (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
US5405519A (en) | 1988-09-15 | 1995-04-11 | New York University | Pulsed oriented electrophoresis |
US5599664A (en) | 1989-04-05 | 1997-02-04 | New York University | Method for characterizing polymer molecules or the like |
US5720928A (en) | 1988-09-15 | 1998-02-24 | New York University | Image processing and analysis of individual nucleic acid molecules |
US6147198A (en) | 1988-09-15 | 2000-11-14 | New York University | Methods and compositions for the manipulation and characterization of individual nucleic acid molecules |
US6150089A (en) | 1988-09-15 | 2000-11-21 | New York University | Method and characterizing polymer molecules or the like |
US6174671B1 (en) | 1997-07-02 | 2001-01-16 | Wisconsin Alumni Res Found | Genomics via optical mapping ordered restriction maps |
US6448047B2 (en) * | 1999-07-30 | 2002-09-10 | Applied Gene Technologies, Inc. | Sample processing to release nucleic acids for direct detection |
US20030036067A1 (en) * | 1989-04-05 | 2003-02-20 | Wisconsin Alumni | Image processing and analysis of individual nucleic acid molecules |
US20030165943A1 (en) * | 2000-06-27 | 2003-09-04 | Uwe Oelmuller | Use of compositions consisting of cationic compounds and proton donors for stabilising and/or isolating nucleic acids in or from micro-organisms such as prokaryots, fungi, protozoa or algae |
US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
US6723510B2 (en) | 1999-07-15 | 2004-04-20 | Qiagen Gmbh | Methods for separating particulate substrates from solution while minimizing particle loss |
US20060223071A1 (en) * | 2005-04-01 | 2006-10-05 | Wisniewski Michele E | Methods, compositions, and kits for detecting nucleic acids in a single vessel |
US20090191566A1 (en) * | 2004-07-30 | 2009-07-30 | Agencourt Bioscience Corporation | Kits for Isolating Nucleic Acids Using Multifunctional Group-Coated Solid Phase Carriers |
US20120164133A1 (en) | 2008-02-19 | 2012-06-28 | Opgen Inc. | Methods of identifying an organism |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL103935A0 (en) * | 1991-12-04 | 1993-05-13 | Du Pont | Method for the identification of microorganisms by the utilization of directed and arbitrary dna amplification |
US7189527B2 (en) * | 1999-09-03 | 2007-03-13 | Bioheap Limited | Bacterial oxidation of sulphide ores and concentrates |
US7255989B1 (en) * | 1999-11-29 | 2007-08-14 | Aventis Pharma S.A. | Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds |
US7595176B2 (en) * | 2003-05-30 | 2009-09-29 | Georgia Tech Research Corporation | Methods and reagents for quantitative analysis of Dehalococcoides species |
DK2260706T3 (en) * | 2005-10-04 | 2016-08-22 | Dsm Ip Assets Bv | Enhanced antifungal composition |
JP2010505425A (en) * | 2006-10-06 | 2010-02-25 | プロメガ コーポレイション | Methods and kits for isolating cells |
WO2009002580A2 (en) * | 2007-04-02 | 2008-12-31 | Boston Medical Center Corporation | Method for bacterial lysis |
US8932809B2 (en) * | 2010-01-19 | 2015-01-13 | Opgen, Inc. | Methods and kits for isolating nucleic acid from an organism |
-
2010
- 2010-01-19 US US12/689,270 patent/US8932809B2/en not_active Expired - Fee Related
- 2010-11-02 CA CA2787639A patent/CA2787639A1/en not_active Abandoned
- 2010-11-02 AU AU2010343249A patent/AU2010343249A1/en not_active Abandoned
- 2010-11-02 WO PCT/US2010/055123 patent/WO2011090529A1/en active Application Filing
- 2010-11-02 EP EP10844146.0A patent/EP2526186A4/en not_active Withdrawn
-
2015
- 2015-01-12 US US14/594,743 patent/US20150132749A1/en not_active Abandoned
Patent Citations (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
US6448012B1 (en) | 1988-09-15 | 2002-09-10 | Wisconsin Alumni Research Foundation | Method for mapping a nucleic acid |
US6509158B1 (en) | 1988-09-15 | 2003-01-21 | Wisconsin Alumni Research Foundation | Image processing and analysis of individual nucleic acid molecules |
US5720928A (en) | 1988-09-15 | 1998-02-24 | New York University | Image processing and analysis of individual nucleic acid molecules |
US6147198A (en) | 1988-09-15 | 2000-11-14 | New York University | Methods and compositions for the manipulation and characterization of individual nucleic acid molecules |
US6150089A (en) | 1988-09-15 | 2000-11-21 | New York University | Method and characterizing polymer molecules or the like |
US5405519A (en) | 1988-09-15 | 1995-04-11 | New York University | Pulsed oriented electrophoresis |
US6294136B1 (en) | 1988-09-15 | 2001-09-25 | Wisconsin Alumni Research Foundation | Image processing and analysis of individual nucleic acid molecules |
US6713263B2 (en) | 1988-09-15 | 2004-03-30 | Wisconsin Alumni Research Foundation | Method for mapping a nucleic acid |
US5599664A (en) | 1989-04-05 | 1997-02-04 | New York University | Method for characterizing polymer molecules or the like |
US20030036067A1 (en) * | 1989-04-05 | 2003-02-20 | Wisconsin Alumni | Image processing and analysis of individual nucleic acid molecules |
US6610256B2 (en) | 1989-04-05 | 2003-08-26 | Wisconsin Alumni Research Foundation | Image processing and analysis of individual nucleic acid molecules |
US6340567B1 (en) | 1997-07-02 | 2002-01-22 | Wisconsin Alumni Research Foundation | Genomics via optical mapping with ordered restriction maps |
US6174671B1 (en) | 1997-07-02 | 2001-01-16 | Wisconsin Alumni Res Found | Genomics via optical mapping ordered restriction maps |
US6723510B2 (en) | 1999-07-15 | 2004-04-20 | Qiagen Gmbh | Methods for separating particulate substrates from solution while minimizing particle loss |
US6448047B2 (en) * | 1999-07-30 | 2002-09-10 | Applied Gene Technologies, Inc. | Sample processing to release nucleic acids for direct detection |
US20030165943A1 (en) * | 2000-06-27 | 2003-09-04 | Uwe Oelmuller | Use of compositions consisting of cationic compounds and proton donors for stabilising and/or isolating nucleic acids in or from micro-organisms such as prokaryots, fungi, protozoa or algae |
US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
US20090191566A1 (en) * | 2004-07-30 | 2009-07-30 | Agencourt Bioscience Corporation | Kits for Isolating Nucleic Acids Using Multifunctional Group-Coated Solid Phase Carriers |
US20060223071A1 (en) * | 2005-04-01 | 2006-10-05 | Wisniewski Michele E | Methods, compositions, and kits for detecting nucleic acids in a single vessel |
US20120164133A1 (en) | 2008-02-19 | 2012-06-28 | Opgen Inc. | Methods of identifying an organism |
Non-Patent Citations (8)
Title |
---|
"Ni-NTA Magnetic Agarose Beads Handbook", June 1998 |
MOCZ ET AL., ANALYTICAL BIOCHEM., vol. 143, no. 2, 1984, pages 283 - 292 |
ORSINI ET AL.: "A microwave-based method for nucleic acid isolation from environmental samples.", LETTERS IN APPLIED MICROBIOLOGY, vol. 33, 2001, pages 17 - 20, XP008161592 * |
RESLEWIC ET AL., APPL ENVIRON ICROBIOL., vol. 71, no. 9, September 2005 (2005-09-01), pages 551 1 - 22 |
SAMAD ET AL., GENOME RES., vol. 5, 1995, pages 1 - 4 |
SAMBROOK ET AL.: "Molecular Cloning. A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY |
SCHICK, ANAL BIOCHEM., vol. 63, no. 2, 1975, pages 345 - 349 |
See also references of EP2526186A4 * |
Also Published As
Publication number | Publication date |
---|---|
EP2526186A1 (en) | 2012-11-28 |
US8932809B2 (en) | 2015-01-13 |
US20150132749A1 (en) | 2015-05-14 |
AU2010343249A1 (en) | 2012-08-09 |
CA2787639A1 (en) | 2011-07-28 |
US20110177521A1 (en) | 2011-07-21 |
EP2526186A4 (en) | 2013-07-10 |
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