WO2011063210A3 - Methods of mapping genomic methylation patterns - Google Patents

Methods of mapping genomic methylation patterns Download PDF

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Publication number
WO2011063210A3
WO2011063210A3 PCT/US2010/057389 US2010057389W WO2011063210A3 WO 2011063210 A3 WO2011063210 A3 WO 2011063210A3 US 2010057389 W US2010057389 W US 2010057389W WO 2011063210 A3 WO2011063210 A3 WO 2011063210A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
methylation patterns
nucleic acid
genomic methylation
mapping genomic
Prior art date
Application number
PCT/US2010/057389
Other languages
French (fr)
Other versions
WO2011063210A2 (en
Inventor
Kevin Clancy
Gavin Meredith
Christopher Adam
Daniel Krissinger
Original Assignee
Life Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Publication of WO2011063210A2 publication Critical patent/WO2011063210A2/en
Publication of WO2011063210A3 publication Critical patent/WO2011063210A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to sample analysis work flows for increasing the efficiency of experiments. Compositions and methods are described for selectively increase the abundance of methylated nucleic acid over non-methylated nucleic acid, followed by analysis of the nucleic acid to identify methylation sites.
PCT/US2010/057389 2009-11-20 2010-11-19 Methods of mapping genomic methylation patterns WO2011063210A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US26319409P 2009-11-20 2009-11-20
US61/263,194 2009-11-20
US44186610P 2010-11-09 2010-11-09
US61/441,866 2010-11-09

Publications (2)

Publication Number Publication Date
WO2011063210A2 WO2011063210A2 (en) 2011-05-26
WO2011063210A3 true WO2011063210A3 (en) 2011-10-13

Family

ID=44060358

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/057389 WO2011063210A2 (en) 2009-11-20 2010-11-19 Methods of mapping genomic methylation patterns

Country Status (1)

Country Link
WO (1) WO2011063210A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2971589C (en) 2014-12-18 2021-09-28 Edico Genome Corporation Chemically-sensitive field effect transistor
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US10811539B2 (en) 2016-05-16 2020-10-20 Nanomedical Diagnostics, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070065824A1 (en) * 2003-07-04 2007-03-22 David Gutig Method for the detection of cytosine methylations in dna by means of cytidine deaminases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070065824A1 (en) * 2003-07-04 2007-03-22 David Gutig Method for the detection of cytosine methylations in dna by means of cytidine deaminases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JANG, KI-HYO ET AL.: "Identification of a Sequence Containing Methylated Cytidine in Corynebacterium glutamicum and Brevibacterium flavum Using Bisulfite DNA Derivatization and Sequencing", JOURNAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. 11, no. 5, 2001, pages 819 - 824, XP009042448 *
LARIJANI, M. ET AL.: "Methylation protects cytidines from AID-mediated deamination", MOLECULAR IMMUNOLOGY, vol. 42, no. 5, March 2005 (2005-03-01), pages 599 - 604, XP004686619, DOI: doi:10.1016/j.molimm.2004.09.007 *
LISTER, R. ET AL.: "Finding the fifth base: genome-wide sequencing of cytosine methylation", GENOME RESEARCH, vol. 19, no. 6, June 2009 (2009-06-01), pages 959 - 66, XP055190057, DOI: doi:10.1101/gr.083451.108 *

Also Published As

Publication number Publication date
WO2011063210A2 (en) 2011-05-26

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