WO2011044394A1

WO2011044394A1 - Purine derivatives useful as hsp90 inhibitors

Info

Publication number: WO2011044394A1
Application number: PCT/US2010/051872
Authority: WO
Inventors: Gabriela Chiosis; Tony Taldone; Weilin Sun
Original assignee: Sloan-Kettering Institute For Cancer Research
Priority date: 2009-10-07
Filing date: 2010-10-07
Publication date: 2011-04-14
Also published as: NZ599138A; EP2486039A1; EA201270535A1; ES2733131T3; BR112012008019A2; PT2486039T; MX340714B; NZ625593A; MX2012004089A; US20120208806A1; JP2013507381A; JP2016145262A; JP2018104451A; EA029272B1; DK2486039T3; CA2776308C; KR102129420B1; CN102639534A; JP6804483B2; KR20180112117A

Abstract

The present application provides substituted purine derivatives and related compounds of the formulas shown. These com ounds are useful as inhibitors of HSP90, and hence in the treatment of related diseases. (Formulae) Z₁-Z₃, X_a-X_c, X₂, X₄, Y and R are as defined in the specification.

Description

PURINE DERIVATIVES USEFUL AS HSP90 INHIBITORS

Statement of Related Cases

This application is related to US Patent Application No. 12/307,063 filed 12/30/2008, and US Patent Application No. 11/814,506, filed July 23, 2007 which are incorporated herein by reference in their entirety for all purposes.

Background of the Invention

This application relates to compounds that inhibit heat shock protein 90 (Hsp90).

The Hsp90 family of proteins has four recognized members in mammalian cells: Hsp90 a and β, Grp94 and Trap-1. Hsp90 a and β exist in the cytosol and the nucleus in association with a number of other proteins. Hsp90 in its various forms is the most abundant cellular chaperone, and has been shown in experimental systems to be required for ATP- dependent refolding of denatured or "unfolded" proteins. It has therefore been proposed to function as part of the cellular defense against stress. When cells are exposed to heat or other environmental stresses, the aggregation of unfolded proteins is prevented by pathways that catalyze their refolding or degradation. This process depends on the association of the unfolded protein in an ordered fashion with multiple chaperones (Hsp 60, 90 and 70 and p23), forming a "refoldosome" and ultimately the ATP-dependent release of the chaperones from the refolded protein.

Hsp90 may also play a role in maintaining the stability and function of mutated proteins. It seems to be required for expression of mutated p53 and v-src to a much greater extent than for their wild-type counterparts. It has been suggested that this occurs as a result of Hsp90-mediated suppression of the phenotypes of mutations that lead to protein unfolding.

Hsp90 is also necessary to the conformational maturation of several key proteins involved in the growth response of the cell to extracellular factors. These include the steroid receptors as well as certain transmembrane kinases (i.e., Raf serine kinase, v-src and Her2). The mechanism whereby Hsp90 affects these proteins is not fully understood, but appears to be similar to its rple in protein refolding. In the case of the progesterone receptor, it has been shown that binding and release of Hsp90 from the receptor occurs in a cyclic fashion in concert with release of other chaperones and immunophilins and is required for high affinity binding of the steroid to the receptor. Thus, Hsp90 could function as a physiologic regulator of signaling pathways, even in the absence of stress.

Hsp90 has been shown to be overexpressed in multiple tumor types and as a function of oncogenic transformation. Whether it plays a necessary role in maintaining transformation is unknown, but it could have at least three functions in this regard. Cancer cells grow in an environment of hypoxia, low pH and low nutrient concentration. They also rapidly adapt to or are selected to become resistant to radiation and cytotoxic chemotherapeutic agents. Thus, the general role of Hsp90 in maintaining the stability of proteins under stress may be necessary for cell viability under these conditions. Secondly, cancer cells harbor mutated oncogenic proteins. Some of these are gain-of-function mutations which are necessary for the transformed phenotype. Hsp90 may be required for maintaining the folded, functionally- active conformation of these proteins. Thirdly, activation of signaling pathways mediated by steroid receptors, Raf and other Hsp90 targets is necessary for the growth and survival of many tumors which thus probably also require functional Hsp90.

Hsp90 has been recognized as a viable target for therapeutic agents. Hsp90 family members possess a unique pocket in their N-terminal region that is specific to and conserved among all Hsp90s from bacteria to mammals, but which is not present in other molecular chaperones. The endogenous ligand for this pocket is not known, but it binds ATP and ADP with low affinity and has weak ATPase activity. The ansamycin antibiotics geldanamycin (GM) and herbimycin (HA) have been shown to bind to this conserved pocket, and this binding affinity has been shown for all members of the Hsp90 family. International Patent Publication No. WO98/51702 discloses the use of ansamycin antibiotics coupled to a targeting moiety to provide targeted delivery of the ansamycin leading to the degradation of proteins in and death of the targeted cells. International Patent Publication No. WOOO/61578 relates to bifunctional molecules having two moieties which interact with the chaperone protein Hsp90, including in particular homo- and heterodimers of ansamycin antibiotics. These bifunctional molecules act to promote degradation and/or inhibition of HER-family tyrosine kinases and are effective for treatment of cancers which overexpress Her-kinases.

Exemplary small molecule therapeutics that bind to the same binding pocket of Hsp90 as ATP and the ansamycin antibiotics are disclosed in PCT Publication Nos.

WO02/36075, WO2006/084030, WO09/042646 and WO09/065035, and US Patent Publications 2005/0113339, 2005/0004026, 2005/0049263, 2005/0256183, 2005/0119292, 2005/0113340, 2005/0107343, 2008/0096903, 2008/0234297, 2008/0234314 and

2008/0253965, all of which are incorporated herein by reference.

Many of these compounds are based on a scaffold of the type disclosed by Chiosis et al in PCT Publication No. WO02/36075, with variations in substituents. In some cases, the compositions can be described by one of the following two general formulas:

wherein Zl, Z2, Z3 are selected from C and N in which numerous options are disclosed for each variable substituent, resulting in an astronomical number of combinations and permutations. In other cases, the compositions can be described by a structural formula in which Xa, Xb, Xc and Xd are not connected to one another but are simply substituents on the benzene ring. These structures have the general formula:

(1C) wherein Zl, Z2, Z3 are selected from C and N. While these compounds are generally active as inhibitors of Hsp90, the level of activity is extremely variable with measured values for EC₅₀ and IC₅₀ being reported in both micromolar and nanomolar ranges. Summary of the Invention

The present application provides compounds useful in the inhibition of Hsp90, and hence in the treatment of disease.

Brief Description of the Figures

Fig. 1 shows examples of unsubstituted aryl groups, including some heterocyclic aryl groups.

Fig. 2 shows examples of unsubstituted heterocyclic groups.

Fig. 3 shows average tumor volume in mice treated with compound 1B-1-HC1 or with vehicle.

Detailed Desciption of the Invention

The present invention provides compounds within the scope of Formula 1 A, IB or 1C with particular combinations of substituents that are effective to inhibit Hsp90. Inhibitors of Hsp90 are recognized as effective in treatments of cancer, and also can be used in the treatment of neurodegenerative diseases as described in PCT Patent Publication

WO2008/005397. WO 2007/14360 discloses the use of Hsp90 inhibitors in treatment of neurofibromatosis Thus, the compounds of the invention can be used in therapeutic methods in the same manner as used other known Hsp90 inhibitors, by administering a therapeutically effective amount of a compound of the invention to an individual, including a human, in need of treatment for cancer, neurodegenerative disease or other condition for which Hsp90 inhibition is relevant.

As used in this application, the term "treatment" refers to delaying the onset of symptoms, reducing the severity or delaying the symptomatic progression of cancer, neurodegenerative disease or other condition in the individual. A cure of the disease is not required to fall within the scope of treatment. Further, it will be appreciated that the specific results of these treatment goals will vary from individual to individual, and that some individuals may obtain greater or lesser benefits than the statistical average for a

representative population. Thus, treatment refers to administration of composition to an individual in need, with the expectation that they will obtain a therapeutic benefit. The term "administering" refers to the act of introducing into the individual the therapeutic compound. In general, any route of administration can be used. Thus, administration by oral, intrathecal, intravenous, intramuscular or parenteral injection is appropriate depending on the nature of the condition to be treated. Administration may also be done to the brain by inhalation because there is a compartment at the upper side of the nose that connects with the brain without having the BBB capillaries. Compounds that cross the blood brain barrier are preferred for this mode of administration, although this

characteristic is not strictly required.

The term "therapeutically effective amount" encompasses both the amount of the compound administered and the schedule of administration that on a statistical basis obtains the result of preventing, reducing the severity or delaying the progression of the disease in the individual. As will be appreciated, preferred amounts will vary from compound to compound in order to balance toxicity/tolerance with therapeutic efficacy and the mode of

administration. Determination of maximum tolerated dose and of the treatment regime in terms of number and frequency of dosing is a routine part of early clinical evaluation of a compound.

In all of the compounds of the present invention, the compound may be as depicted, or as a pharmaceutically acceptable salt or ester thereof.

In naming options for X₂, X4 and , the name refers to the type of group that is directly attached to the central structure, which group may include additional functionality. Thus, "alky group refers to a linear, cyclic or branched saturated hydrocarbon, for example a hydrocarbon having from 1 to 10 carbon atoms, in which the atom directly attached to the central structure is a carbon atom. Such an alkyl group may include substituents other than hydrogen, for example an oxygen-containing group including without limitation hydroxyl and alkoxy; a halogen group; a nitrogen-containing group including without limitation amino, amido and alkylamino; an aryl group; a sulfur-containing group including without limitation thioalkyl; and/or a non-aromatic cyclic group including heterocycles and carbocycles.

Carbon atoms in these substituents may increase the total number of carbon atoms in the alkyl group to above 10 without departing from the invention. All references to alkyl groups in the specification and claims hereof encompass both substituted and unsubstituted alkyl groups unless the context is clearly to the contrary. "AlkenyV group refers to a linear, cyclic or branched hydrocarbon, for example a hydrocarbon having from 1 to 10 carbon atoms, and at least one double bond, in which the atom directly attached to the central structure is a carbon atom. The alkenyl group may include any of the substituents mentioned above for an alkyl group. All references to alkenyl groups in the specification and claims hereof encompass both substituted and unsubstituted alkenyl groups unless the context is clearly to the contrary.

"Alkynyl" group refers to a linear, cyclic or branched hydrocarbon, for example a hydrocarbon having from 1 to 10 carbon atoms, and at least one triple bond, in which the atom directly attached to the central structure is a carbon atom. The alkynyl group may include any of the substituents mentioned above for an alkyl group. All references to alkynyl groups in the specification and claims hereof encompass both substituted and unsubstituted alkynyl groups unless the context is clearly to the contrary.

"AryF' group refers to any group derived from a simple aromatic ring. Aryl group includes heteroaryl. (See Fig. 1) Aryl groups may be substituted or unsubstituted. When X₂, X4 and R is identified as an aryl group, an atom of the aryl ring is bound directly to an atom of the central structure. An aryloxy substituent is an aryl group connected to the central structure through an oxygen atom. The aryl group may include any of the substituents mentioned above for an alkyl group, and in addition an aryl group may include an alkyl, alkenyl or alkynyl group. AH references to aryl groups in the specification and claims hereof encompass both substituted and unsubstituted aryl groups unless the context is clearly to the contrary.

"Amino" group refers to any group which consists of a nitrogen attached by single bonds to carbon or hydrogen atoms. In certain instances, the nitrogen of the amino group is directly bound to the central structure. In other instances, an amino group may be a subtituent on or within a group, with the nitrogen of the amino group being attached to the central structure through one or more intervening atoms. Examples of amino groups include NH₂, alkylamino, alkenylamino groups and N-containing non-aromatic heterocyclic moiety (i.e., cyclic amines). Amino groups may be substituted or unsubstituted. All references to amino groups in the specification and claims hereof encompass substituted and unsubstituted amino groups unless the context is clearly to the contrary.

"Halogen " group refers to fluorine, chlorine, bromine or iodine. "Heterocyclic" group refers to a moiety containing at least one atom of carbon, and at least one atom of an element other than carbon, such as sulfur, oxygen or nitrogen within a ring structure. These heterocyclic groups may be either aromatic rings or saturated and unsaturated non-aromatic rings. Some examples are given in Fig. 2. Heterocylic groups may be substituted or unsubstituted. All references to heterocyclic groups in the specification and claims encompass substituted and unsubstituted heterocyclic groups unless the context is clearly to the contrary.

In the compounds of the invention, all of the atoms have sufficient hydrogen or non- hydrogen substituents to satisfy valence, or the compound includes a pharmaceutically acceptable counterion, for example in the case of a quaternary amine.

In the structures set forth below examples are provided in which all of Zl , Z2 and Z3 are nitrogen. These examples are intended as exemplary, and are not intended to exclude options in which one or more of Zl, Z2 and Z3 is carbon. In particular, corresponding compositions in which Z2 or Z3 is carbon are considered to be within the scope of this disclosure.

A. Structures of formula 1 A in which Xa or Xb is O

In accordance with a first embodiment of the invention, the compounds have general formula 1A, in which one of Xa or Xb is O, and Xc and the other of Xa and Xb is CH_2. Thus, the compounds of this embodiment may be represented by the general formula

(2)

wherein:

one of Xa and Xb is O and the other is -CH_:

Y is -CH₂- or -S-,

X4 is hydrogen or halogi X₂ and R are in combinations as discussed below.

A-I. In some embodiments of the invention, X₂ is halogen. In these embodiments, R is suitably a primary aminoalkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R is not a piperidino moiety.

Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3- (cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2- (methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl,

cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3 -( 1 H-imidazoyl) propyl .

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 1 A and IB (Compounds lA-1 to 1 A-4, 1A-6, l A-11, 1 A-18 to 1A-28, 1A-30, 1A-31, 1A-49, lB-1 to 1B-5, 1B-18, 1B-23 to 1B-25, lB-29 to 1B-32, 1Β-34,1Β-35, 1B-37, 1B-49, 1B-52 1B-56 andlB-57).

As shown, in preferred embodiments of formula (2), is H, chlorine or fluorine.

In a particular preferred embodiment of formula (2), Y is S, is H, X₂ is I.

In a particular preferred embodiment of formula (2), Y is CH₂, 4 is CI, X₂ is I.

In a particular preferred embodiment of formula (2), Y is CH₂, X4 is F, X₂ is I.

In a particular preferred embodiment of formula (2), Y is S, is F, X₂ is I.

Tests were run on compounds in accordance with the first embodiment of the invention, and the EC₅₀ for Hsp90 binding in JNPL3 brain cell lysate and SKBr3 cell lysate was assessed. The results are summarized in Table 2 A. While all of the compounds had desirable low EC₅₀ values, both compounds tested in which Xb is O were superior to the related compound in which Xa is O. In addition, compound lB-1 was superior to an otherwise identical compound in which both Xa and Xb are O (PU-HZ150 disclosed in US 12/307,063) which has an EC₅₀ of 12-14.4 nM and compound 1B-2 was superior to an otherwise identical molecule in which both Xa and Xb are O (PU-H71 disclosed in US 11/814,506) which has an EC₅₀ of 30.8-54 nM in the same experimental system.

Animal Studies. Four- to 6-week-old nu/nu athymic female mice were obtained from Taconic Farms. Experiments were carried out under an Institutional Animal Care and Use Committee approved protocol, and institutional guidelines for the proper and humane use of animals in research were followed. Before administration, a solution of 1B-1-HC1 was formulated in PBS (pH 7.4). All mice received Augmentin (amoxicillin/clavulanate potassium; SmithKline Beecham) in their drinking water while on therapy. Mice were killed by C0₂ euthanasia.

Mice bearing MDA-MB-468 tumors (n=5) reaching a volume of 100-150 mm³ were treated i.p. (i.p.) using 1B-1-HC1 at 50 mg/kg 3 x week or vehicle. Tumor volume was determined by measurement with Vernier calipers, and tumor volume was calculated as the product of its length x width² x0.4. Tumor volume was expressed on indicated days as the median tumor volume ± SD indicated for groups of mice. The average tumor volumes are summarized in Fig. 3. As shown, in the 35 days of the experiment the average tumor volume in the control mice increased by about a factor of 3X, while the tumor size in the mice treated with 1 B- 1 -HC1 decreased.

Table 2B shows measured values for EC₅₀ in JNPL3 brain cell lysates for compounds 1B-3, 1B-4 and 1B-25 in accordance with this embodiment of the invention which incorporates a fluorine as X₄ and in which Y is -CH₂-. Desirably low values of EC₅₀ were observed.

In addition, Table 2B shows measured values for EC₅₀ in JNPL3 brain cell lysates for compound 1B-24 in accordance with this embodiment of the invention which incorporates a hydrogen as X₄ and in which Y is S. Desirably low values of EC₅₀ were observed.

A-II. In some embodiments of the invention, X₂ is an aryl group. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. Table 8 provides a summary of the R groups from these patents and applications. In some embodiments within this group, R includes a heteroatom, such as nitrogen, oxygen or sulfur.

In some embodiments, the heteroatom is nitrogen. In some of these embodiments, R is a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3-(lH-imidazoyl) propyl.

In embodiments of formula (2) within this group, R has the formula:

site of 9N-attachment

where Rj is selected from COH, COMe, COEt, COraPr, COz^'Pr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

In embodiments of the invention within this group, R has the formula site of 9N-attachment

H H M

X-(CH_{2) m}-N-(CH₂)_n-|^-S-NH₂

O

II

X-(CH₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1 -6. In embodiments within this group, R has the formula site of 9N-attachment

where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is an aromatic heterocycle.

In specific embodiments, X₂ is a furan, thiophene, pyrazole, imidazole, pyrrole, oxazole or thiazole.

In specific embodiments, X₂ is a furan, thiophene, pyrazole, imidazole, oxazole or thiazole.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is 2-, 3-furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is CH₂, 4 is CI, X₂ is 2-, 3- furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is 2-, 3-furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is S, is F, X₂ is 2-, 3-furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is S, is H, X₂ is 2- or 3-thiophene. In particular embodiments of formula (2), Y is CH₂, 4 is CI, X₂ is 2- or 3- thiophene. In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is 2- or 3-thiophene. In particular embodiments of formula (2), Y is S, is F, X₂ is 2- or 3-thiophene. In particular embodiments of formula (2), Y is S, is H, X₂ is 2- or 3-pyrazole. In particular embodiments of formula (2), Y is CH₂, is CI, X₂ is 2- or 3- pyrazole. In particular embodiments of formula (2), Y is CH₂, is F, X₂ is 2- or 3-pyrazole. In particular embodiments of formula (2), Y is S, is F, X₂ is 2- or 3-pyrazole. In particular embodiments of formula (2), Y is S, is H, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl. In particular embodiments of formula (2), Y is CH₂, ¾ is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazoryl..

In particular embodiments of formula (2), Y is CH₂, ¾ is F, X₂ 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, ¾ is F, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, X* is H, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl. ^"

In particular embodiments of formula (2), Y is CH₂, X* is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, X* is F, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, X^ is F, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In these particular embodiments, R may be any one of the types of groups described above.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 1A and IB (Compounds 1A-5, 1A-7, 1A-12, 1A-13, 1A-15 to 1A-17, l A- 36, 1A-37, 1A-42, 1A-45 to 1A-48, lA-50 to 1A-52, 1B-6, 1B-8, lB-12, 1B-14, 1B-15, 1B- 17, 1B-26, 1B-27, 1B-33, lB-42 to 1B-44, lB-46, 1B-50, 1B-51, 1B-53 to 1B-55). As shown, in preferred embodiments of formula (2) in which X is an aryl group, X^ is H, chlorine or fluorine.

Table 2C shows measured values for EC₅₀ in JNPL3 brain cell lysates for compounds 1B-26 and 1B-27 in accordance with this embodiment of the invention which incorporates a hydrogen as X^ and in which Y is S. Desirably low values of EC₅₀ were observed.

A-IIL In some embodiments of the invention, X₂ is an alkynyl group. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. See Table 8.

In some embodiments within this group, R includes a heteroatom, such as nitrogen, oxygen or sulfur. In some embodiments, the heteroatom is nitrogen. In some of these embodiments, R is a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl . Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3 -(lH-imidazoyl) propyl.

In embodiments of formula (2) within this group, R has the formula:

site of 9N-attachment

where Rj is selected from COH, COMe, COEt, COrcPr, COz^'Pr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

In embodiments of the invention within this group, R has the formula site of 9N-attachme

O

II

X-(CH₂)n-0-S-NH₂

O

where m= 2-3 and n= 1-6.

In embodiments within this group, R has the formula site of 9N-attachment

where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, X₄ is H, X₂ is acetylene.

In particular embodiments of formula (2), Y is CH₂, X₄ is CI, X₂ is acetylene.

In particular embodiments of formula (2), Y is CH₂, X₄ is F, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is acetylene.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 1 A and IB (Compounds 1A-8, 1 A- 14, 1A-29, 1A-32 to 1A-35, lB-10, 1B-13, 1B-16, 1B-19, 1B-28, 1B-36, 1B-38 to 1B-41, 1B-45 1B-47 and 1B-58). As shown, in preferred embodiments of formula (2) in which X₂ is an alkynyl group, X is H, chlorine or fluorine.

Table 2D shows a measured value for ECso in J PL3 brain cell lysates for compound 1B-28 in accordance with this embodiment of the invention which incorporates a hydrogen as X₄ and in which Y is S. A desirably low value of ECso was observed.

A-IV. In some embodiments of the invention, X₂ is a cyano group. In these embodiments, R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl , and with the proviso that R is not a piperidino moiety. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3-(lH-imidazoyl) propyl.

In a particular preferred embodiment of formula (2), Y is S, ¾ is H, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is CH₂, ¾ is CI, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is CH₂, ¾ is F, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is S, ¾ is F, X₂ is CN.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Table 1 A and IB (Compounds 1 A-9, lB-11 and 1B-20).

A-V. In some embodiments of the invention, X₂ is an amino group. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. See Table 8.

In some embodiments within this group, R includes a heteroatom, such as nitrogen, oxygen or sulfur.

In some embodiments, the heteroatom is nitrogen. In some of these embodiments, R is a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl . Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3 -( 1 H-imidazoyl) propyl .

In embodiments of formula (2) within this group, R has the formula:

where Ri is selected from COH, COMe, COEt, CO«Pr, COzPr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

H H fl

X-(CH_{2) m}-N-(CH₂)_n-N-S-NH₂

O

o

X-(CH ₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1-6.

In embodiments within this group, R has the formula site of 9N -attachment

where m= 2-3 and n= 1-6.

In a particular preferred embodiment of formula (2), Y is S, X4 is H, X₂ is dimethylamino

In a particular preferred embodiment of formula (2), Y is CH₂, X4 is CI, X₂ is dimethylamino

In a particular preferred embodiment of formula (2), Y is CH , X4 is F, X₂ is dimethylamino.

In a particular preferred embodiment of formula (2), Y is S, X4 is F, X₂ is

dimethylamino. In a particular preferred embodiment of formula (2), Y is S, X₄ is H, X₂ is aziridino. In a particular preferred embodiment of formula (2), Y is CH₂, X₄ is CI, X₂ is aziridino_.

In a particular preferred embodiment of formula (2), Y is CH₂, X₄ is F, X₂ is aziridino.

In a particular preferred embodiment of formula (2), Y is S, X₄ is F, X₂ is aziridino.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 1 A and IB (Compounds lA-10, 1A-38 to 1A-41, 1A-43, 1A-44, 1B-7, 1B-21 and 1B-48).

A- VI. In some embodiments of the invention, X₂ is a cycloalkyl or a cycloalkenyl. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. See Table 8.

In some embodiments, the heteroatom is nitrogen. In some of these embodiments, R is a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl . Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydrOxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) .ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3 -(lH-imidazoyl) propyl.

In embodiments of formula (2) within this group, R has the formula: site of 9N-attachment

where Rj is selected from COH, COMe, COEt, CO«Pr, COzPr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

H H M

X-(CH_{2) m}-N-(CH₂)_n-N-S-NH₂

O

X-(CH₂)_n-0-S-NH₂

O

m= 2-3 and n= 1-6.

In embodiments within this group, R has the formi site of 9N-attachment

where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is a cycloalkyl with one ring.

In specific embodiments, X₂ is a cyclopropane, a cyclobutane or a cyclopentane.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is cyclopentyl.

In these particular embodiments, R may be any one of the types of groups described above. Specific examples of compounds in accordance with this embodiment of the invention are listed in Table IB (Compounds 1B-9, and 1B-22) although the embodiment is not limited to the option in which Xb is O, and includes compounds in which Xa is O.

B. Structures of formula 1 A in which Xa or Xb is S

In accordance with a second embodiment of the invention, the compounds have general formula 1A, in which one of Xa or Xb is S, and Xc and the other of Xa and Xb is CH_2. Thus, the compounds of this embodiment may be represented by the general formula

(2)

wherein:

one of Xa and Xb is S and the other is -CH₂-;

Y is -CH₂- or -S-,

X₄ is hydrogen or halogen; and

X₂ and R are as discussed below.

In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. See Table 8.

In some embodiments, the heteroatom is nitrogen. In some of these embodiments, R is a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl . In some of these embodiments, R is a primary, secondary or tertiary alkyl-amino-alkyl, alkyl-aryl, or an alkyl-nonaromatic heterocycle. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, . 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2- (hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyramino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3- (hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2- (methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3-(lH-imidazoyl) propyl.

In embodiments of formula (2) within this group, R has the formula:

site of 9N-attachment

where Ri is selected from COH, COMe, COEt, CO«Pr, COz^'Pr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

H H M

X-(CH₂₎m-N-(CH₂)_n-N-S- NH₂

O

II

X -(CH₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1-6.

In embodiments within this group, R has the formula site of 9N -attachment

where m= 2-3 and n= 1-6.

In embodiments of formula (2) in which Xa or Xb is S, X₂ may be any group shown to be attached to the same position as X₂ in any of the compounds disclosed herein or in the various patents and patent applications cited above. Specifically, X₂ may be alkyl, alkenyl, alkynyl, aryl, cycloalkyl, cycloalkenyl, saturated or unsaturated heterocycle, aryl, halogen, aryloxy, alkoxy, halogenated alkoxy, alkenyloxy, hydroxyalkyl, amino, alkylamino, dialkylamino, acylamino, carbamyl, amido, dialkylamido, alkylamido, alkylsulfonamido, sulfonamido, trihalocarbon, -thioalkyl, S0₂-alkyl, -COO-alkyl,- COalkyl, OH, N0₂, CN or alkyl-CN, or part of a ring formed by R;

In particular embodiments of the invention, X₂ is halogen, and R is an (alkylamino) alkyl or (dialkylamino) alkyl.

In particular embodiments, X₂ is halogen and R is 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(Ν^ο ηο1ϊηο) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In some embodiments of the invention, X₂ is an aryl group.

In particular embodiments, X₂ is aryl and R is 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(TST-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl. In some embodiments of the invention, X₂is a heteroaryl group.

In some embodiments, X₂ is a heteroaryl group and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(TSi-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments, X₂ is 2- or 3-furan and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments, X₂ is 2- or 3-thiophene and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl- amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments, X₂ is 3-pyrazolyl and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In some embodiments of the invention, X₂ is an alkynyl group.

In some embodiments of the invention, X₂ is an alkynyl group, and R is 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments of the invention, X₂ is acetylene, and R is 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In some embodiments of the invention, X₂ is CN.

In particular embodiments of the invention, X₂ is CN, and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl- amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In some embodiments of the invention, X₂ is an amine.

In some embodiments of the invention, X₂ is an amine, and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl- amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments of the invention, X₂ is dimethyl amine, azetidino or aziridino, and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3- (neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3- (isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2- (hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N- morpholino) propyl, or 3-(hydroxyethyl, isopropyl amino) propyl.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 1C and ID.

C. Structures of formula 1A in which Xa, Xb and Xc are all carbon

In accordance with a third embodiment of the invention, the compounds have general formula 1 A, in which Xa, Xb and Xc are all carbon, connected by two single or one single bond and one double bond, and wherein

Y is -CH₂- or -S-;

X4 is hydrogen or halogen; and

X and R are in combinations as discussed below.

C-I. In some embodiments of the invention in which Xa, Xb and Xc are all carbon, X₂ is halogen.

In some embodiments within this group, R includes a heteroatom, such as nitrogen, oxygen or sulfur with the proviso that R does not include a piperidino moiety. In some embodiments, R is suitably an optionally substituted primary alkyl-amino, an optionally substituted secondary or tertiary alkyl-amino-alkyl, alkyl-aryl, or an alkyl- nonaromatic heterocycle, with the proviso that R is not a piperidino moiety.

In some embodiments, the heteroatom is nitrogen. In some of these embodiments, R is a primary, secondary or tertiary alkyl-amino-alkyl, alkyl-aryl, or an alkyl-nonaromatic heterocycle, with the proviso that R does not include a piperidino moiety. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2- (hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3- (hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3 -(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2- (methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3-(lH-imidazoyl) propyl.

O

II

X-(CH₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1-6.

In embodiments within this group, R has the formula site of 9N-attach

where m= 2-3 and n= 1-6.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables IE (Compounds lE-1 to 1E-4, lE-6, lE-18, 1E-21, 1E-23 to 1E-26, 1E- 35, 1E-38, 1E-39, 1E-42 to 1E-48, 1E-68 to 1E-76). As shown, in preferred embodiments of formula (2), ¾ is H, chlorine or fluorine.

In a particular preferred embodiment of formula (2), Y is S, X₄ is H, X₂ is I.

In a particular preferred embodiment of formula (2), Y is CH₂, ¾ is CI, X₂ is I.

In a particular preferred embodiment of formula (2), Y is CH₂, X₄ is F, X₂ is I.

In a particular preferred embodiment of formula (2), Y is S, X₄. is F, X₂ is I.

Table 2E shows measured values for EC₅₀ in JNPL3 brain and SKBr3 cell lysates for compounds 1E-2, 1E21 and 1E-23 in accordance with this embodiment of the invention which incorporates a hydrogen as X₄. and in which Y is S. Desirably low values of EC₅₀ were observed.

C-II. In some embodiments of the invention, X₂ is an aryl group. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. See Table 8.

In some embodiments, the heteroatom is nitrogen. In some of these embodiments, R is a primary arnino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3-( 1 H-imidazoyl) propyl.

In embodiments of formula (2) within this group, R has the formula:

site of 9N-attachment

H H fl

X-(CH_{2) m}-N-(CH₂)_n-N-S-NH₂

O

o

X-(CH₂)_n-0-S-MH₂

O

where m= 2-3 and n= 1-6.

In embodiments within this group, R has the formula site of 9N-attachment

where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is an aromatic heterocycle.

In specific embodiments, X₂ is a furan, thiophene, pyrazole, imidazole, pyrrole, oxazole and thiazole.

In specific embodiments of the X₂ is a furan, thiophene, pyrazole, oxazole and thiazole or imidazole. In specific embodiments, X₂ is an aromatic heterocycle.

In particular embodiments of formula (2), Y is S, X₄ is H, X₂ is 2-, 3-furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is CH₂, X₄ is CI, X₂ is 2-, 3- furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is CH₂, X₄ is F, X₂ is 2-, 3-furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is 2-, 3-furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is S, X₄ is H, X₂ is 2- or 3-thiophene.

In particular embodiments of formula (2), Y is CH₂, X₄ is CI, X₂ is 2- or 3- thiophene.

In particular embodiments of formula (2), Y is CH₂, X₄ is F, X₂ is 2- or 3-thiophene.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is 2- or 3-thiophene.

In particular embodiments of formula (2), Y is S, X₄ is H, X₂ is 2- or 3-pyrazole.

In particular embodiments of formula (2), Y is CH₂, X₄ is CI, X₂ is 2- or 3- pyrazole.

In particular embodiments of formula (2), Y is CH₂, X₄ is F, X₂ is 2- or 3-pyrazole.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is 2- or 3-pyrazole.

In particular embodiments of formula (2), Y is S, X₄ is H, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, X₄ is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, X₄ is F, X₂ 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, X₄ is H, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl. In particular embodiments of formula (2), Y is CH₂, X₄ is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, X₄ is F, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables IE (Compounds 1E-5, 1E-7, lE-11 to 1E-13, 1E-15 to 1E-17, 1E-27, 1E- 29 to 1E-33, 1E-36, 1E-37, 1E-41, 1E-59 to 1E-76, 1E-84 and 1E-85). As shown, in preferred embodiments of formula (2) in which X₂ is an aryl group, is H, chlorine or fluorine.

C-III. In some embodiments of the invention, X₂ is an alkynyl group. In these embodiments, R may be as described above in Section CII.

In specific embodiments, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, X₄ is H, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, X₄ is F, X is acetylene.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables IE (Compounds 1E-8, 1E-14, 1E-19. 1E-20, 1E-22, 1E-40, 1E-49 to 1E- 58 and 1E-77 to 1E-82). As shown, in preferred embodiments of formula (2) in which X₂ is an alkynyl group, X₄ is H, chlorine or fluorine.

Table 2F shows a measured value for EC₅₀ in JNPL3 brain cell lysates for compound 1E-22 in accordance with this embodiment of the invention which incorporates a hydrogen as X₄ and in which Y is S. A desirably low value of EC₅₀ was observed. C-TV. In some embodiments of the invention, X₂ is a cyano group. In these embodiments, R may be as described above in Section C-II, with the proviso that R does not include a piperidino moiety.

In a particular preferred embodiment of formula (2), Y is S, X₄ is H, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is CH₂, X₄ is F, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is S, X4 is F, X₂ is CN.

A specific example of a compound in accordance with this embodiment of the invention is listed in Table IE (Compound 1E-9).

C-V. In some embodiments of the invention, X₂ is an amino group. In these particular embodiments, R may be any one of the types of groups described in Section C-II.

In a particular preferred embodiment of formula (2), Y is S, X₄ is H, X₂ is azetidino.

In a particular preferred embodiment of formula (2), Y is CH₂, is CI, X₂ is azetidino.

In a particular preferred embodiment of formula (2), Y is CH₂, X₄ is F, X₂ is azetidino.

In a particular preferred embodiment of formula (2), Y is S, is F, X₂ is azetidino. Specific examples of compounds in accordance with this embodiment of the invention are listed in Table IE (Compound IE- 10, 1E-28 and 1E-34).

C-VI. In some embodiments of the invention, X is a cycloalkyl or a cycloalkenyl. In these particular embodiments, R may be any one of the types of groups described in Section C-II.

In specific embodiments, X₂ is a cycloalkyl with one ring.

In specific embodiments, X₂ is a cyclopropane, cyclobutane or cyclopentane.

In particular embodiments of formula (2), Y is S, X4 is H, X is cyclopentyl.

In particular embodiments of formula (2), Y is CH₂, L4 is CI, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is C¾, X₄ is F, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is cyclopentyl. In these particular embodiments, R may be any one of the types of groups described above.

D. Structures of formula 1 A in which Xa or Xb is N

In accordance with a fourth embodiment of the invention, the compounds have general formula 1 A, in which one of Xa or Xb is N, and Xc and the other of Xa and Xb are CH₂. The N may be unsubstituted (i.e. NH) or substituted, for example with methyl, ethyl, acetyl. Thus, the compounds of this embodiment may be represented by the general formula

(2)

wherein:

one of Xa and Xb is N bonded to H or a substituent, and the other is -CH₂-;

Y is -CH₂- or -S-,

X₄ is hydrogen or halogen; and

X₂ and R are as discussed below.

In these embodiments, R may be any of the groups disclosed as a substituent at the 9- position nitrogen herein or in the various patents and applications cited above. See Table 8. In some embodiments, R includes a nitrogen heteroatom. In further embodiments, R is any of the options discussed above in Section B.

X₂ may be any group shown to be attached to the same position in any of the compounds disclosed in the various patents and patent applications cited above, or as described above in Section B.

In particular embodiments of the invention, X₂ is halogen, and R is an amino alkyl, an alkylaminoalkyl, dialkylaminoalkyl, or trialkylammonioalkyl group, in which each alkyl portion may be linear, cyclic or branched, or an alkyl heterocycle, where the alkyl may be bound to a nitrogen in the heterocyclic group. Specific R groups include without limitation

2- (methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3- (ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3- (allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, and 3-(lH-imidazoyl) propyl.

In some embodiments of the invention, X₂ is an aryl group.

In particular embodiments, X₂ is aryl and R is 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3-(hydroxyethyl, isopropyl amino) propyl.

In some embodiments of the invention, X₂ is a heteroaryl group.

In some embodiments, X₂ is a heteroaryl group and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl,

3 - (ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments, X₂ is 2- or 3-furan and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments, X₂ is 2- or 3-thiophene and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl- amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments, X₂ is 3-pyrazolyl and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl, or 3 -(hydroxy ethyl, isopropyl amino) propyl.

In some embodiments of the invention, X₂ is an alkynyl group.

In some embodiments of the invention, X₂ is an alkynyl group, and R is 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3-(hydroxyethyl, isopropyl amino) propyl. In particular embodiments of the invention, X₂ is acetylene, and R is 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino) propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3-(hydroxyethyl, isopropyl amino) propyl.

In some embodiments of the invention, X₂ is CN.

In particular embodiments of the invention, X₂ is CN, and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl- amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3-(hydroxyethyl, isopropyl amino) propyl.

In some embodiments of the invention, X₂ is an amine.

In some embodiments of the invention, X₂ is an amine, and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl- amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino) propyl, 3-(cyclopentyl, methyl amino) propyl, 3 -(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3-(hydroxyethyl, isopropyl amino) propyl.

In particular embodiments of the invention, X₂ is dimethyl amine or aziridino, and R is 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3- (ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(N-morpholino) propyl or 3- (hydroxyethyl, isopropyl amino) propyl.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables IF and 1G.

E. Structures of formula 1 A in which Xa or Xb is carbonyl or thiocarbonyl

In accordance with a fifth embodiment of the invention, the compounds have general formula 1 A, in which one of Xa or Xb is carbonyl (C=0) or thiocarbonyl (C=S), and Xc and the other of Xa and Xb is CH_2. Thus, the compounds of this embodiment may be represented by the general formula

(2)

wherein:

one of Xa and Xb is C=0 or C=S, and the other is -CH₂-;

Y is -CH₂- or -S-,

X is hydrogen or halogen; and

X₂ and R are as discussed below.

In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein (See Table 8), with the proviso that when X₂ is halogen or CN, R does not include a piperidino moiety.

In some embodiments within this group, R includes a heteroatom, such as nitrogen, oxygen or sulfur. In some embodiments, the heteroatom is nitrogen. In these embodiments, R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-aryl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino) propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3-(lH-imidazoyl) propyl.

In embodiments of formula (2) within this group when X₂ is thiocarbonyl, R may have the formula:

where Ri is selected from COH, COMe, COEt, CO«Pr, COr^'Pr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

where m= 2-3 and n= 1-6.

In embodiments within this group, R has the formula site of 9N-attachment

where m= 2-3 and n= 1-6.

X₂ may be any substituent shown to be attached at the same position in any of the compounds disclosed in the various patents and patent applications cited above, or as described above in Section B.

In particular embodiments of the invention, X is halogen, and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In some embodiments of the invention, X₂ is an aryl group.

In particular embodiments, X₂ is aryl and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3- (cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2- (methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl,

cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -(lH-imidazoyl) propyl.

In some embodiments of the invention, X₂ is a heteroaryl group.

In some embodiments, X₂ is a heteroaryl group and R is suitably a primary amino- alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle- alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In particular embodiments, X₂ is 2- or 3-furan and R is suitably a primary amino- alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle- alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3 -(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3 -(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -( 1 H-imidazoyl) propyl . In particular embodiments, X₂ is 2- or 3-thiophene and R is suitably a primary amino- alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle- alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3 -(hydroxy ethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -( 1 H-imidazoyl) propyl .

In particular embodiments, X₂ is 3-pyrazolyl and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3- (cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2- (methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl,

cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -(1 H-imidazoyl) propyl.

In some embodiments of the invention, X₂ is an alkynyl group.

In some embodiments of the invention, X₂ is an alkynyl group, and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a

nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, and 3-(lH-imidazoyl) propyl.

In particular embodiments of the invention, X₂ is acetylene, and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, and 3-(lH-imidazoyl) propyl.

In some embodiments of the invention, X₂ is CN.

In particular embodiments of the invention, X₂ is CN, and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R does not include a piperidino moiety. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3- (hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2- (methyl, isobutyl amino) ethyl, and 3-(lH-imidazoyl) propyl.

In some embodiments of the invention, X₂ is an amine.

In some embodiments of the invention, X₂ is an amine, and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3 -(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3 -(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, and 3-(lH-imidazoyl) propyl.

In particular embodiments of the invention, X₂ is dimethyl amine or aziridino, and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3 -(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3 -(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, and 3-(lH-imidazoyl) propyl.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 1H and II.

F. Structures of formula 1A in which Xa, Xb and Xc include at least one non- carbon atom and -Xa-Xc-Xb- includes a double bond

In accordance with a sixth embodiment of the invention, the compounds have general formula 1 A, with Xa, Xc, Xb and the bonds between them selected from a combination in the following table:

Xa Bond Xc bond Xb

C double C single 0

C double C single N ^'

C double C single S

0 single C double C

N single C double C

s single C double C

^"N double C single 0

N double C single s

N single C double N

0 single C double N

s single C double N

N double N single 0 N double N single S

N double N single C

0 single N double N

N single N double N

S single N double N

C single N double N

Y is -CH₂- or -S-,

¾ is hydrogen or halogen; and

X₂ and R are as discussed below.

In these embodiments, R may be any of the groups disclosed as a substituent at the imposition nitrogen herein or in the various patents and applications cited above. In some embodiments, R includes a nitrogen heteroatom. In further embodiments, R is any of the options discussed above in Section B.

X₂ may be any group shown to be attached at the same position in any of the compounds disclosed in the various patents and patent applications cited above, or as described above in B.

In particular embodiments of the invention, X₂ is halogen, and R is an aminoalkyl (alkylamino) alkyl or (dialkylamino) alkyl.

In particular embodiments, X₂ is halogen and R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, . Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3- (cyclopentyl,, methyl amino) propyl, 3-(ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3 -(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2- (methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl,

cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In some embodiments of the invention, X₂ is an aryl group.

In particular embodiments, X₂ is aryl and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, . Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -( 1 H-imidazoyl) propyl .

In some embodiments of the invention, X₂ is a heteroaryl group.

In some embodiments, X₂ is a heteroaryl group and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-( 1 H-imidazoyl) propyl.

In particular embodiments, X₂ is 2- or 3-furan and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3- (hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2- (methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -(1 H-imidazoyl) propyl.

In particular embodiments, X₂ is 2- or 3-thiophene and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, -3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -(1 H-imidazoyl) propyl.

In some embodiments of the invention, X₂ is an alkynyl group, and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3- (ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3- (allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In particular embodiments of the invention, X₂ is acetylene, and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3- (ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3- (allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In some embodiments of the invention, X₂ is CN.

In particular embodiments of the invention, X₂ is CN, and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In some embodiments of the invention, X₂ is an amine.

In some embodiments of the invention, X₂ is an amine, and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl -amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -( 1 H-imidazoyl) propyl .

In particular embodiments of the invention, X₂ is dimethyl amine or aziridino, and R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3- (allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 3A-3F.

G. Structures of formula 1A in which Xa and Xb are both O

In accordance with a seventh embodiment of the invention, the compounds have general formula 1 A, in which Xa and Xb are O and Xc is CH₂. Thus, the compounds of this embodiment may be represented by the general formula

(2)

wherein:

in which Xa and Xb are O,

Y is -CH₂- or -S-,

X₄ is hydrogen or halogen; and

X₂ and R are in combinations as discussed below.

G-I. In some embodiments of the invention, X₂ is an aryl group. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. See Table 8.

In some embodiments, the heteroatom is nitrogen. In some of these embodiments, R is suitably a primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t- butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2- (isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t- butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl, and 3-(lH-imidazoyl) propyl.

In embodiments of formula (2) within this group, R has the formula:

site of 9N-attachment

where Rl is selected from COH, COMe, COEt, CO«Pr, COzPr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

H H ¾

X-(CH_{2) m}-N-(CH₂)_n-N-S-NH

O

o

X-(CH₂)_n-0-|-NH₂

O

where m= 2-3 and n= 1-6.

In embodiments within this group, R has the site of 9N-attachment

where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is a heterocycle.

In specific embodiments, X₂ is phenyl, furan, thiophene, pyrazole, imidazole, thiazole, oxazole or pyrrole.

In specific embodiments of the X₂ is phenyl, furan, methylfuran, thiophene, pyrazole, thiazole, oxazole or imidazole.

In particular embodiments of formula (2), Y is S, ¾ is H, X₂ is 2-, 3-furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is CH₂, X is CI, X₂ is 2-, 3- furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is CH₂, is F, X₂ is 2-, 3-furan or 5- methyl-2 - furanyl .

In particular embodiments of formula (2), Y is CH₂, is CI, X₂ is 2- or 3- thiophene.

In particular embodiments of formula (2), Y is CH₂, 4 is F, X₂ is 2- or 3-thiophene.

In particular embodiments of formula (2), Y is CH₂, 4 is CI, X₂ is 2- or 3- pyrazole.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is 2- or 3-pyrazole.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is 2- or 3-pyrazole.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, 4 is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, is F, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl. In particular embodiments of formula (2), Y is S, ¾ is H, X₂ is optionally substituted phenyl.

In particular embodiments of formula (2), Y is CH₂, ¾ is CI, X₂ is optionally substituted phenyl.

In particular embodiments of formula (2), Y is CH₂, ¾ is F, X₂ is optionally substituted phenyl.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is optionally substituted phenyl.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is optionally substituted pyridine.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is optionally substituted pyridine.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is optionally substituted pyridine.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is optionally substituted pyridine.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is optionally substituted isooxazole.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is optionally substituted isooxazole.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is optionally substituted isooxazole.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is optionally substituted isooxazole.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is optionally substituted imidazole.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is optionally substituted imidazole.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is optionally substituted imidazole. In particular embodiments of formula (2), Y is S, ¾ is F, X₂ is optionally substituted imidazole.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 4A, 4C, 4D, 4F, 4G and 4H. As shown, in preferred embodiments of formula (2) in which X2 is an aryl group, ¾ is H, chlorine or fluorine.

Table 2G shows measured values for EC₅o in JNPL3 brain cell lysates and in SKBr3 cell lysate for compounds 4A-1 to 4A-8, 4C1 to 4C-11, 4C14, 4C-16, 4C-38 to 4C-41, 4D-1 to 4D-3, 4D-16, 4D-17, 4F-1, 4G-1 to 4G-7, 4G-9, 4H-1 to 4H-7 in accordance with this embodiment of the invention which incorporates a hydrogen as ¾ and in which Y is S or a fluorine as ¾ and in which Y is -CH₂-. Desirably low values of EC₅₀ were observed for several examples.

G-II. In some embodiments of the invention, X₂ is an alkynyl group. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. See Table 8.

In some embodiments, when X₂ is alkynyl, R includes a nitrogen heteroatom.

In a further embodiment, when X₂ is alkenyl, R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl.

Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In embodiments of formula (2) when X₂ is alkynyl, R has the formula:

site of 9N-attachment

where Ri is selected from COH, COMe, COEt, COrcPr, COz^'Pr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

In embodiments of the invention when X₂ is alkynyl, R has the formula site of 9N-attachment

O

H H M

X-(CH_{2) m}-N-(CH₂)_n-N-S- NH₂

O

o

X -(CH₂)_n-0-S-MH₂

O

where m= 2-3 and n= 1-6.

In embodiments when X₂ is alkynyl, R has the forr site of 9N-attachment

where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is acetylene.

In particular embodiments of formula (2), Y is CH₂, 4 is CI, X₂ is acetylene.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is acetylene. In these particular embodiments, R may be any one of the types of groups described above.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 4B. As shown, in preferred embodiments of formula (2) in which X₂ is an alkynyl group, ¾ is H, chlorine or fluorine.

Table 2H shows measured values for EC50 in JNPL3 brain cell lysates and in SKBr3 cell lysate for compounds 4B-1 to 4B-4, 4B13 and 4B-14 in accordance with this

embodiment of the invention which incorporates a hydrogen as ¾ and in which Y is S, or a fluorine as ¾ and in which Y is -CH₂-. Desirably low values of EC50 were observed.

G-III. In some embodiments of the invention, X₂ is a cyano or cyanoalkyl group. In these embodiments, R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino) propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3- (hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2- (methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In a particular preferred embodiment of formula (2), Y is CH₂, is CI, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is CH₂, X4 is F, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is S, is F, X₂ is CN. In other embodiments in which X₂ is cyano or cyanoalkyl group, R is s ite o f 9N-att achment

H H S

X-(CH_2)M-N-(CH₂)_N-N-S-NH₂

O

X-(CH₂)_N-0-|-NH₂

O

where m= 2-3 and n= 1-6, or

site of 9N -attachment

where m= 2-3 and n= 1-6.

A specific example of a compound in accordance with this embodiment of the invention is listed in Table 4E.

Table 21 shows measured values for EC₅₀ in JNPL3 brain cell lysates and in SKBr3 cell lysate for compounds (4E-1 to 4E-4) in accordance with this embodiment of the invention which incorporates a hydrogen as ¾ and in which Y is S or a fluorine as ¾ and in which Y is -CH₂-. Desirably low values of EC₅₀ were observed for several examples.

G-IV. In some embodiments of the invention, X₂ is a cycloalkyl (saturated carbocyclic) or cycloalkenyl. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen in this application, or in the various patents and patent applications cited herein. (See Table 8)

In some embodiments within this group, R includes a nitrogen heteroatom.

In a further embodiment within this group, R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino)-propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isbpropyl amino) propyl, • 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl-, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl; 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In embodiments of formula (2) within this group, R has the formula:

site of 9N-attachment

O

II

X-(CH₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1-6.

In embodiments when X₂ is aryl, R has the formula site of 9N -attach

where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is a cycloalkyl with one ring. In specific embodiments, X₂ is a cyclopropane, cyclobutane or cyclopentane.

In particular embodiments of formula (2), Y is S, ¾ is H, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is CH₂, ¾ is F, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is S, X_t is F, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is CH₂, _t is CI, X₂ is cyclopentyl. In these particular embodiments, R may be any one of the types of groups described above.

Specific examples of compounds of this type are shown in Table 41.

Table 2J shows measured values for EC₅₀ in J PL3 brain cell lysates and in SKBr3 cell lysate for compound 41-12 in accordance with this embodiment of the invention which incorporates a hydrogen as ¾ and in which Y is S. Desirably low values of EC₅₀ were observed for several examples.

Table 2G shows results for EC₅₀ measured in SKBr3 breast cancer cells and J PL3 brain cell lysates for compounds listed in Table 4A. As shown, compounds of this type are generally more active with respect to brain cancer cells. As shown, the greatest activity was observed for compound 4A-1, in which there is no substituent on the X₂ phenyl group, and the least activity is seen for compounds 4A-3 and 4A-4 in which electron withdrawing CF₃ substituents are at the meta positions.

An embodiment of the invention therefore has the structure (2) shown above, in which Y is S, ¾ is hydrogen, X₂ is phenyl, optionally substituted at the para position, and R includes a nitrogen heteroatom, and therefore is an alkylamino, an (alkylamino) alkyl or (dialkylamino) alkyl. Preferred R groups of this type are as listed above.

Examples of compounds within this seventh embodiment of the invention, in which X₂ is alkynyl are shown in Table 4B. Table 2H shows results for EC₅₀ for Hsp90 binding in JNPL3 brain cell lysates. As shown, all of the compounds tested were active, however, those with an acetylene substituent, such as 4B-1, 4B-4, 4B-13 and 4B-14, were most active.

Examples of compounds within this seventh embodiment of the invention, in which X₂ is an aryl group containing an oxygen atom are shown in Table 4C. Specific suitable substituent groups are 2-furanyl, 3-furanyl and 5-methyl-2-furanyl.

Table 2G shows results for EC₅₀ for Hsp90 binding in SKBr3 breast cancer cells and J PL3 brain cell lysates for some of the compounds shown in Table 4C. All of the compounds show good activity in both experimental systems. In Table 4C, compound 4-Cl 1 has X₂= isoxazole, whereas 4C-11, 4C-38 and 4C-39 have X₂= oxazolyl, including both a nitrogen and an oxygen. Compounds with X₂ = 2-oxazolyl (4C-38 and 4C-39) were more active than those with X₂ = iso-oxazolyl (4C-11).

Examples of compounds within this seventh embodiment of the invention, in which X₂ is. an aryl group containing a sulfur atom in the aryl ring are shown in Table 4D. Table 2G shows results for EC₅₀ for Hsp90 binding in SKBr3 breast cancer cells and JNPL3 brain cell lystates for some of the compounds shown in Table 4D. All of the compounds show good activity in both experimental systems. In Table 4D, compounds 4D-16 and 4D-17 have X₂= 2-thiazolyl, including both a nitrogen and an oxygen.

Examples of compounds within this seventh embodiment of the invention, in which X is -CN or cyanoalkyl are shown in Table 4E. Table 21 shows results for EC ₀ for Hsp90 , . binding in JNPL3 brain cell lystates for the two -CN compounds shown in Table 4E. Both of the compounds show good activity in both experimental systems.

An example of a compound within this seventh embodiment of the invention, in which X₂ is a 6-membered aryl ring containing a nitrogen atom in the aryl ring, with the proviso that there is not also an oxygen in the ring, is shown in Table 2 (labeled as 4F-1). In Compound 4F-1, X₂ is 4-pyridinyl. X₂ could also be 2-pyridinyl, 3-pyridinyl, or pyrazinyl, 4- pyrimidinyl, 5- pyrimidinyl, 3-pyridazinyl or 4-pyridazinyl.

EQo for Hsp90 binding in SKBr3 breast cancer cells and JNPL3 brain cells were determined for compound 4F-1 to be 9,620 nM and 4,120 nM, respectively.

Examples of compounds within this seventh embodiment of the invention, in which X₂ is a 5-membered aryl rings containing 2 nitrogens in the ring are shown in Table 4G. The specific X₂ groups shown are 3-, 4- and 5-pyrazolyl. Other examples of X₂ groups in this category are 4- or 5-imidazolyl.

EC o for Hsp90 binding in SKBr3 breast cancer cells and JNPL3 brain cell lysates were determined for some of the compounds listed in Table 4G. The results are summarized in Table 2K. Particularly good results were observed for compounds 4G-3, 4G-6 and 4G-9 in which the substituent is a 3-pyrazolyl. Examples of compounds within this seventh embodiment of the invention, in which X₂ is a pyrrolyl group are shown in Table 4H. The specific X groups shown are 2 or 3- pyrrolyl.

EC₅₀ for Hsp90 binding in SKBr3 breast cancer cells and JNPL3 brain cell lysates were determined for some of the compounds listed in Table 4H. The results are summarized in Table 2L.

H. Structures of formula IB in which Xa and Xb are O

In accordance with an eighth embodiment of the invention, the compounds have general formula IB, in which both Xa and Xb are O, and Xc and Xd are CH₂. Thus, compounds of this embodiment are represented by the formula:

R

(3)

wherein

Y is -CH₂-, -O- or -S-;

is hydrogen or halogen; and

X₂ and R are as discussed below.

H-L In some embodiments of compounds in accordance with formula (3), X₂ is halogen. In these embodiments, R is suitably an amino-alkyl, a secondary or tertiary alkyl- amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R does not include a piperidino moiety. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In specific embodiments of the invention, X₂ is I.

In particular embodiments of the invention X₂ is I, Y is S and ¾ is H.

Specific examples of compounds within this group are shown in Table 5A.

Examples of compounds within this group, in which X₂ is a halogen, are shown in Table 5A (5A-1 to 5A-19). Table 2M shows EC₅₀ values for binding of Hsp90 in JNPL3 brain cells for some of the compounds of Table 5 A. All show values of less than 100 nM.

H-II. In some embodiments of compounds in accordance with formula (3), X₂ is aryl. In these embodiments, R may be any of the groups disclosed as a substituent at the imposition nitrogen in this application, or in the various patents and patent applications cited herein. See Table 8.

In some embodiments, when X₂ is aryl, R includes a nitrogen heteroatom.

In these embodiments, R is suitably an amino-alkyl, a secondary or tertiary alkyl-amino- alkyl, a trialkylarnmonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl.

Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2 -(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino) propyl, 3- (cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2- (methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl,

cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3 -(lH-imidazoyl)^" propyl.

In embodiments of formula (2) when X₂ is aryl, R has the formula:

In embodiments of the invention when X₂ is aryl, R has the formula site of 9N-attachment

O

I I

X-(CH_{2) m}-N-(CH₂)_n-N-S-NH₂

O

o

X-(CH₂)_n-0-S-NH₂

O

m= 2-3 and n= 1-6.

In embodiments when X₂ is aryl, R has the formul site of 9N -attachment

where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is an aromatic heterocycle.

In specific embodiments, X₂ is a furan, thiophene, pyrazole, imidazole, pyrrole, oxazole or thiazole. In specific embodiments, X₂ is a furan, thiophene, pyrazole, imidazole, oxazole or thiazole.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is 2-, 3- furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is 2-, 3-furan or 5- methyl-2-furanyl .

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is 2-, 3-furan or 5- methyl-2-furanyl.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is 2- or 3 -thiophene.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is 2- or 3- thiophene.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is 2- or 3 -thiophene.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is 2- or 3-thiophene.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is 2- or 3-pyrazole.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is 2- or 3- pyrazole.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, X4 is F, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula_(2), Y is CH₂, X4 is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl. In particular embodiments of formula (2), Y is CH₂, X₄ is F, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

Examples of compounds in which X₂ is an aryl group containing an oxygen atom in the aryl ring, are shown in Table 5B.

Table 2N shows measured values for EC₅₀ in JNPL3 brain cell lysates for compounds 5B-1, 5B-7, 5B-33 and 5B-34 in accordance with this embodiment of the invention which incorporates a fluorine as X₄ and in which Y is -CH₂- or a hydrogen as X₄ and in which Y is S. Desirably low values of EC₅₀ were observed.

Examples of compounds in which X₂ is an aryl group containing a sulfur atom in the aryl ring are shown in Table 5C.

Examples of compounds in which X₂ is a 5-membered aryl rings containing 2 nitrogens in the ring are shown in Table 5D.

Table 20 shows measured values for EC50 in JNPL3 brain cell lysates for compounds 5D-2 and 5D-4 in accordance with this embodiment of the invention which incorporates a fluorine as X4 and in which Y is -CH₂- or a hydrogen as X4 and in which Y is S. Desirably low values of EC50 were observed.

An embodiment of the invention has the structure shown in formula (3), in which Y is -CH_2- or S, X4 is hydrogen of fluorine, X₂ is X₂ is a pyrazolyl, particularly a 3-pyrazolyl, or an imidazolyl. Preferred R groups of this type are as listed above.

H-III. In some embodiments of the invention, X₂ is an alkynyl group. In these embodiments, R may be as described above in Section H-II.

In specific embodiments, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is acetylene.

In particular embodiments of formula (2), Y is CH₂, X4 is CI, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is acetylene. In these particular embodiments, R may be any one of the types of groups described above.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Tables 5E. As shown, in preferred embodiments of formula (2) in which X₂ is an alkynyl group, ¾ is H, chlorine or fluorine.

H-IV. In some embodiments of the invention, X₂ is a cyano group. In these embodiments, R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R does not contain a piperidino moiety. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino) propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3- (allyl, methyl amino) propyl, 3 -(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In a particular preferred embodiment of formula (2), Y is CH₂, X4 is CI, X₂ is CN.

In other embodiments in which X₂ is cyano or cyanoalkyl group, R is

site of 9N-attachment

H H «

X-(CH_{2) m}-N-(CH₂)_n-N-S-NH₂

O

X-(CH₂)_n-0-S-NH₂

O where m= 2-3 and n= 1-6, or

site of 9N -attachment

where m= 2-3 and n= 1-6.

Specific examples of compounds in accordance with this embodiment of the invention are listed in Table 5F.

H-V. In some embodiments of the invention, X₂ is an amino group. In these particular embodiments, R may be any of the R groups described in Section H-II.

In a particular preferred embodiment of formula (2), Y is S, ¾ is H, X₂ is azetidino.

In a particular preferred embodiment of formula (2), Y is CH₂, X₄ is CI, X₂ is azetidino_.

In a particular preferred embodiment of formula (2), Y is CH₂, is F, X₂ is azetidino_.

In a particular preferred embodiments of formula (2), Y is S, is F, X₂ is azetidino. A specific examples of a compounds in accordance with this embodiment of the invention is listed in Table 5G

H-VI. In some embodiments of the invention, X₂ is a cycloalkyl or cycloalkenyl. In these particular embodiments, R may be any of the R groups described in Section H-II.

In specific embodiments, X₂ is a cycloalkyl with one ring.

In specific embodiments, X₂ is a cyclopropane, cyclobutane or cyclopentane.

In particular embodiments of formula (2), Y is S, X4 is H, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is CH₂, X₄ is CI, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is CH₂, X₄ is F, X₂ is cyclopentyl.

In particular embodiments of formula (2), Y is S, X4 is F, X₂ is cyclopentyl.

A specific examples of a compounds in accordance with this embodiment of the invention is listed in Table 5H I. Structures of formula IB in which Xa and/or Xb is a heteroatom, but not both O

In accordance with a ninth embodiment of the invention, the compounds have general formula IB, in which one or both of Xa or Xb is a heteroatom such as O, N or S, with the proviso that both of Xa and Xb are not O (see Section H). Specific examples of compounds within this group are shown in Table 6A.

I-L In some embodiments of compounds in accordance with formula (3), X₂ is halogen. In these embodiments, R is suitably an amino-alkyl, a secondary or tertiary alkyl- amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R is not a piperidino moiety. .

Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2- (methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl -amino) ethyl, 2- (ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2- (isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino)- propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3-(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In specific embodiments of the invention, X₂ is I.

In particular embodiments of the invention X₂ is I, Y is S and X₄ is H.

I-IL In some embodiments of compounds in accordance with formula (3), X₂ is aryl.

In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen herein or in the various patents and applications cited above. See Table 8.

In some embodiments, when X₂ is aryl, R includes a nitrogen heteroatom. In these embodiments, R is suitably an amino alkyl, a secondary or tertiary alkyl- amino-alkyl, a trialkylammonioalkyl group, aryl-alkyl, or a nonaromatic heterocycle-alkyl. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3 -(isopropyl amino) propyl, 3 -(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino) propyl, 3- (cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2- (methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3 -(propyl,

cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(l H-imidazoyl) propyl.

In embodiments of formula (2) when X₂ is aryl, R has the formula:

where Rj is selected from COH, COMe, COEt, COwPr, CO/Pr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

(CH_{2) m}-N-(CH₂)_n-N-S-NH₂

II

X-(CH₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1 -6.

In embodiments when X₂ is aryl, R has the formula site of 9N -attachment

H

-(CH2_)m-N-(CH2)_n-?

H -OH where m= 2-3 and n= 1-6.

In specific embodiments, X₂ is an aromatic heterocycle.

In particular embodiments of formula (2), Y is S, X₄ is F, X₂ is 2-, 3-furan or 5- methyl-2-furanyi.

In particular embodiments of formula (2), Y is CH₂, X₄ is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl. In particular embodiments of formula (2), Y is CH₂, ¾ is F, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, Xt is F, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is S, Xt is H, X₂ is 2-thiazolyl, 5-methyl- 2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, X_t is CI, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

In particular embodiments of formula (2), Y is CH₂, ¾ is F, X₂ is 2-thiazolyl, 5- methyl-2-thiazolyl, 2-oxazolyl or 5-methyl-2-oxazolyl.

I-III. In some embodiments of the invention, X₂ is an alkynyl group. In these embodiments, R may be as described above in Section I-II.

In specific embodiments, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, Xt is H, X₂ is acetylene.

In particular embodiments of formula (2), Y is CH₂, t is CI, X₂ is acetylene.

In particular embodiments of formula (2), Y is CH₂, Xt is F, X₂ is acetylene.

In particular embodiments of formula (2), Y is S, Xt is F, X₂ is acetylene.

I-IV. In some embodiments of the invention, X₂ is a cyano group. In these embodiments, R is suitably an amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an arylralkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R does not include a piperidino moiety. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino) propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3- (allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2-(cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3 -(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In a particular preferred embodiment of formula (2), Y is CH₂, X₄ is CI, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is CH₂, is F, X₂ is CN.

In a particular preferred embodiment of formula (2), Y is S, is F, X₂ is CN.

In other embodiments in which X₂ is cyano or cyanoalkyl group, R is

site of 9N-attachment

H H 11

X-(CH_{2) m}-N-(CH₂)_n-N-S-NH₂

O

X-(CH₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1-6, or

site of 9N -attachment

where m= 2-3 and n= 1-6.

I-V. In some embodiments of the invention, X₂ is an amino group. In these particular embodiments, R may be any of the R groups described in Section I-II.

In a particular preferred embodiment of formula (2), Y is S, is H, X₂ is aziridino.

In a particular preferred embodiment of formula (2), Y is CH₂, is CI, X₂ is aziridino. In a particular preferred embodiment of formula (2), Y is CH , ¾ is F, X₂ is aziridino. In a particular preferred embodiment of formula (2), Y is S, X₄ is F, X₂ is aziridino.

J. Structures of formula IB in which Xa. Xc. Xd and Xb are all carbon

In accordance with a tenth embodiment of the invention, the compounds have general formula IB, in which Xa, Xc, Xd and Xb are all carbon connected by single or double bonds. In these embodiments, R may be any of the groups disclosed as a substituent at the 9-position nitrogen herein or in the various patents and applications cited above. (See Table 8)

In some embodiments, when X₂ is aryl, R includes a nitrogen heteroatom.

In these embodiments, R is suitably an aminoalkyl, alkylaminoalkyl,

dialkylaminoalkyl, or trialkylammonioalkyl group, in which each alkyl portion may be linear, cyclic or branched, or an alkyl heterocycle, where the alkyl may be bonded to a nitrogen in the heterocyclic group. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl- amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3- (cyclopentylamino) propyl, 3-(cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3- (ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3 -(methyl, isopropyl amino) propyl, 3 -(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3 -(methyl, propargyl amine) propyl, 2-(methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl, cyclopropylmethyl amino) propane, 3-(hydroxyethyl, cyclohexyl amino) propyl, 2- (cyclopropylmethyl amino) ethyl, 2-(methyl, isobutyl amino) ethyl, 3-(N-morpholino) propyl and 3-(lH-imidazoyl) propyl.

In embodiments of formula (2) when X is aryl, R has the formula:

site of 9N-attachment

where Ri is selected from COH, COMe, COEt, COwPr, COz^'Pr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature. In embodiments of the invention when X₂ is aryl, R has the formula site of 9N-attachment

H H fl

X-(CH_{2) m}-N-(CH₂)_n-N-S-NH₂

O

X-(CH₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1-6.

In embodiments when X₂ is aryl, R has the formula site of 9N-attachment

where m= 2-3 and n= 1-6.

Specific examples of compounds within this embodiment of the invention are shown in Table 6B.

Table 2P shows measured values for EC₅o in JNPL3 brain cell lysates for compounds 6B-25 in accordance with this embodiment of the invention which incorporates a hydrogen as X4 and in which Y is S. Desirably low values of EC50 were observed.

K. Structures of formula 1C

In some embodiments of the present invention having the general formula 1C, the compounds of the invention can be represented by the general formula:

(4)

in which

Rl is alkyl, for example methyl or ethyl,

Y is S or CH₂,

X4 is H or halogen,

X₂ is saturated or unsaturated non-aromatic carbocycle or heterocycle, aryl, alkylamino, dialkylamino, alkynyl or part of a ring formed by R; and

R is hydrogen, alkyl, alkenyl, or alkynyl, linear, branched or cyclic, optionally including heteroatoms such as N, S or O, optionally connected to the 2'-position to form an 8 to 10 member ring.

In some embodiments, R is suitably an alkylaminoalkyl, dialkylaminoalkyl, or trialkylammonioalkyl group, in which each alkyl portion may be linear, cyclic or branched, or an alkyl heterocycle, where the alkyl may be bonded to a nitrogen in the heterocyclic group. Specific R groups include without limitation 2-(methyl, t-butyl- amino) ethyl, 2-(methyl, isopropyl amino) ethyl, 3-(neopentyl amino) propyl, 2-(isobutyl-amino) ethyl, 2-(ethyl, isopropyl amino) ethyl, 3-(isopropyl amino) propyl, 3-(t-butyl- amino) propyl, 2-(isopropyl amino) ethyl, 2-(hydroxyethyl, isopropyl amino) ethyl, 3-(cyclopentylamino) propyl, 3- (cyclopentyl, methyl amino) propyl, 3-(ethylamino) propyl, 3 -(ethyl, methyl amino) propyl, 2-(neopentyl amino) ethyl, 3-(methyl, isopropyl amino) propyl, 3-(ethyl, isopropyl amino) propyl, 3-(hydroxyethyl, isopropyl amino) propyl, 3-(methyl, propargyl amine) propyl, 2- (methyl, propargyl amine) ethyl, 3-(allyl, methyl amino) propyl, 3-(propyl,

In embodiments of formula (3), R has the formula: site of 9N-attachment

where Ri is selected from COH, COMe, COEt, COwPr, COzPr, S0₂Me, CH₂OX where X can be H or oxygen, nitrogen, sulphur or halogen containing alkyl of linear, branched or cyclic nature.

In embodiments of formula (3), R has the formula site o f 9N-att achment

H H ¾

X-(CH_{2) m}-N-(CH₂)_n- -S-NH₂

O

X-(CH₂)_n-0-S-NH₂

O

where m= 2-3 and n= 1-6.

In embodiments of formula (3) R has the formula site of 9N -attachment

where m= 2-3 and n= 1-6.

In some embodiments, X₂ is alkynyl, such as acetylene. Examples of such structures are shown in Table 7A

In some embodiments, X₂ is cyano or cyanomethyl. Examples of such structures are shown in Table 7B.

In some embodiments, X₂ is a heterocycle, including without limitation aziridine, azetidine, oxetane, thietane, tetrahydrofuran, tetrahydropyrrole, tetrahydrothiophane, imidazolidine, oxazolidine, thiazolidine, azirine, oxirine, pyrroline, pyrrole, dihydrofuran, furan, dihydrothiophene, thiophene, pyrazole, imidazole, oxazole, isoxazole, thiazole, isothiazole, 1 ,2,3-triazole, 1,2,4-triazole, dithiazole, tetrazole, pyridine, pyran, thiine, diazine, thiazine, dioxin, triazine, tetrazine. Examples of such structures are shown in Tables 7C-7F. Table 2Q shows measured values for EC50 in JNPL3 brain and SKBr3 cell lysates for compounds (7A-20, 7C-13, 7D-3 and 7E-6) in accordance with this embodiment of the invention which incorporates a hydrogen as ¾ and in which Y is S. Desirably low values of EC50 were observed, although X₂ = -OCH₃ was observed to be less active than X₂ = -0-CH₂- O- in every shown instance: 4C-5 vs 7C-13 (6.5 nM vs 84nM), 4D-3 vs 7D-3 (18.5nM vs 240nM) and 4G-9 vs 7E-6 (5.5nM vs 32nM).

L. Structures in which X¾ is alkynyl

In accordance with a further aspect of the present invention, compounds are provided in accordance with any of formulas (1 A), (IB) or (1C) in which X₂ is alkynyl. In these compounds,

Y is CH₂, S, O, C=0, C-S, N or any other linking group disclosed in a compound with the same scaffold structure herein or in the patents and patent applications cited above;

X₄ is H or halogen or any other group disclosed at this position in a compound with the same scaffold structure herein or in the patents and patent applications cited above;

Xa, Xb, Xc and Xd are any group(s) disclosed at this position herein or in the patents and patent applications cited above; and

R is any R group disclosed at this position of the same scaffold structure herein or in the patents and patent applications cited above. (See Table 8)

In some embodiments, Zl, Z2, and Z3 are all nitrogen.

In some embodiments, Zl and Z3 are nitrogen and Z2 is carbon.

In some embodiments, Z3 is carbon.

M. Structures in which X₂ is Furan, Thiophene. Pyrazole, Oxazole or

Thiazole

In accordance with a further aspect of the present invention, compounds are provided in accordance with any of formulas (1 A), (IB) or (1C) in which X₂ is a furan, thiophene, 3- pyrazole, oxazole or thiazole. In these compounds,

Y is CH₂, S, O, C=0, C=S, N or any other linking group disclosed in a compound with the same scaffold structure herein or in the patents and patent applications cited above; X4 is H or halogen or any other group disclosed at this position in a compound with the same scaffold structure herein or in the patents and patent applications cited above;

R is any R group disclosed at this position of the same scaffold structure herein or in the patents and patent applications cited above.

In some embodiments, Zl, Z2, and Z3 are all nitrogen.

In some embodiments, Zl and Z3 are nitrogen and Z2 is carbon.

In some embodiments, Z3 is carbon.

Synthetic Methods

Compounds in accordance with formulas (1 A) and (IB) can be made through the application of the following methodologies.

5 or 6 member ring

(a) Cul, neocuproine, NaOf-Bu, DMF, 110 °C; (b) NIS, acetonitrile, RT; (c) Cs₂C0₃, 1 ,2-dibromoethane or 1 ,3- dibromopropane; (d) HNR₁R₂, DMF, rt; (e) X₂M, Pd (cat.), DMF, 50-100 °C.

Scheme 1.

5 or 6 member ring

(a) triphenyl phosphite, pyridine, microwave; (b) HF-pyridine, NaN0₂; (c) NIS, acetonitrile, rt; (d) Cs₂C0₃, 1 ,2-dibromoethane or 1 ,3-dibromopropane; (e) HNR₁R₂, DMF, rt; (f) X₂M, Pd (cat.), DMF, 50-100 °C.

Scheme 2.

General Methods: 1H and ¹³C NMR spectra were recorded on a Bruker 500 MHz instrument. Chemical shifts are reported in δ values in ppm downfield from TMS as the internal standard. 1H data are reported as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, br = broad, m = multiplet), coupling constant (Hz), integration. ¹³C chemical shifts are reported in δ values in ppm downfield from TMS as the internal standard. High resolution mass spectra were recorded on a Waters LCT Premier system. Low resolution mass spectra were obtained on a Waters Acquity Ultra Performance LC with electrospray ionization and SQ detector. High-performance liquid chromatography analyses were performed on a Waters Autopurification system with PDA, MicroMass ZQ, and ELSD detector, and a reversed phase column (Waters X-Bridge C 18, 4.6 x 150 mm, 5 μπι) using a gradient of; method A (a) H₂0 + 0.1% TFA and (b) CH₃CN + 0.1% TFA, 5 to 95% b over 10 minutes at 1.2 mL/min; method B (a) H₂0 + 0.1 % TFA and (b) CH₃CN + 0.1% TFA, 20 to 90% b over 16 minutes at 1.0 mL/min. Column chromatography was performed using 230-400 mesh silica gel (EMD).

Specific compounds were synthesized as follows;

PU-H71

Reagents and conditions: (a) RB(OH)₂, PdCl₂(PPh₃)₂, NaHC0₃, H₂0, DMF, 90°C.

Scheme 3. Suzuki coupling of PU-H71.

9-(3-(isopropylamino)propyl)-8-(6-phenylbenzo [d] [1,3] dioxol-5-ylthio)-9H-purin-6- amine [DZ2-388]. Phenylboronic acid (10.7 mg, 0.0876 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (0.5 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 10:1) to give 19.8 mg (73%) of DZ2-388. 1H NMR (500 MHz, MeOH-d₄) δ 8.14 (s, 1H), 7.28-7.34 (m, 3H), 7.17-7.21 (m, 2H), 7.12 (s, 1H), 6.90 (s, 1H), 6.09 (s, 2H), 4.03 (t, J= 6.4 Hz, 2H), 3.27 (septet, J= 6.6 Hz, 1H), 2.72 (t, J= 6.6 Hz, 2H), 2.13 (m, 2H), 1.40 (d, J= 6.5 Hz, 6H); ¹³C NMR (125 MHz, MeOH-<¾ 6 156.0, 153.4, 152.1, 151.1, 150.3, 149.4, 142.3, 141.8, 130.4, 129.1, 128.7, 120.3, 119.8, 1 15.7, 1 12.2, 103.8, 52.2, 43.2, 41.1, 27.6, 19.3; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₄H₂₇N₆0₂S, 463.1916; found 463.1905; HPLC: method A R_t = 6.50, method B R_t = 7.40.

8-(6-(4-ieri-butylphenyl)benzo[d] [l,31dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine [DZ2-390]. 4-tert-Butylphenylboronic acid (15.6 mg, 0.0876 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (0.5 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 3 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC

(hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:5:2:1) to give 25.0 mg (83%) of DZ2-390. 1H NMR (500 MHz, MeOH-i¾ δ 8.11 (s, lH), 7.27 (d, J= 8.4 Hz, 2H), 7.14 (s, lH), 7.12 (d, J = 8.4 Hz, 2H), 6.86 (s, 1H), 6.06 (s, 2H), 3.93 (t, J= 6.9 Hz, 2H), 2.92 (septet, J= 6.5 Hz, 1H), 2.61 (t, J= 7.3 Hz, 2H), 1.86 (m, 2H), 1.28 (s, 9H), 1.12 (d, J= 6.5 Hz, 6H); ¹³C NMR (125 MHz, MeOH-i¾ δ 155.9, 153.3, 151.9, 151.8, 150.9, 150.2, 149.2, 141.9, 138.8, 130.0, 125.9, 120.4, 120.3, 115.4, 112.3, 103.6, 50.6, 44.0, 41.8, 35.4, 31.8, 29.3, 21.1; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₈H₃₅N₆0₂S, 519.2542; found 519.2545; HPLC: method A R_t = 7.43, method B R_t = 9.45.

8-(6-(3,5-bis(trifluoromethyl)phenyl)benzo[d][l,3]dioxol-5-ylthio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine [DZ2-391]. 3,5-

Bis(trifluoromethyl)phenylboronic acid (22.6 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (0.5 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 3 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:5:2:1) to give 24.4 mg (70%) of DZ2-391. 1H NMR (500 MHz, CDC1₃) δ 8.22 (s, 1H), 7.79 (s, 3H), 7.12 (s, 1H), 6.86 (s, 1H), 6.09 (s, 2H), 5.71 (br s, 2H), 4.07 (t, J= 6.6 Hz, 2H), 2.82 (septet, J= 6.2 Hz, 1H), 2.49 (t, J= 6.5 Hz, 2H), 1.95 (m, 2H), 1.12 (d, J= 6.3 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 154.1, 152.3, 151.5, 149.6, 148.6, 147.5, 142.1, 137.2, 131.2 (q, J = 33 Hz), 129.6, 123.1 (q, J= 270 Hz), 121.3, 119.6, 119.4, 114.9, 110.8, 102.4, 49.5, 42.8, 40.6, 28.8, 21.7; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₆H₂₅F₆N₆0₂S, 599.1664; found 599.1653; HPLC: method A R_t = 7.45, method B R_t = 9.38. 8- (6-(4-(dimethylamino)phenyl)benzo[d] [l,3]dioxol-5-ylthio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine [DZ2-392] . 4-(Dimethylamino)phenylboronic acid (14.5 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 3 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:5:2:1) to give 25.3 mg (85%) of DZ2-392. 1H NMR (500 MHz, CDC1₃) δ 8.24 (s, 1H), 7.13 (d, J= 8.7 Hz, 2H), 6.93 (s, 1H), 6.83 (s, 1H), 6.67 (d, J= 8.7 Hz, 2H), 6.01 (br s, 2H), 5.98 (s, 2H), 4.02 (t, J= 6.7 Hz, 2H), 2.97 (s, 6H), 2.78 (septet, J= 6.3 Hz, 1H), 2.44 (t, J- 6.7 Hz, 2H), 1.87 (m, 2H), 1.10 (d, J= 6.3 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 154.4, 152.4, 151.5, 149.9, 148.5, 148.0, 147.1, 139.6, 130.0, 127.8, 120.5, 119.7, 112.8, 111.7, 111.0, 101.7, 49.3, 43.1, 40.9, 40.4, 28.9, 21.9; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₆H₃₂N₇0₂S, 506.2338; found 506.2330; HPLC: method A R_t = 5.72, method B R_t = 5.12.

9- (3-(isopropylamino)propyl)-8-(6-(thiophen-2-yl)benzo[d|[l,3]dioxol-5-ylthio)-9H- purin-6-amine [DZ2-395]. 2-Thienylboronic acid (11.2 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 12.5:1) to give 12.4 mg (45%) of DZ2-395. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 8.17 (s, 1H), 7.33 (dd, J= 1.4, 4.9 Hz, 1H), 7.07 (s, 1H), 7.04 (s, 1H), 7.00-7.02 (m, 2H), 6.09 (s, 2H), 4.12 (t, J= 6.6 Hz, 2H), 2.95 (septet, J= 6.6 Hz, 1H), 2.62 (t, J= 6.8 Hz, 2H), 2.00 (m, 2H), 1.19 (d, J= 6.6 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-i¾ δ 154.2, 152.0, 151.1, 149.4, 148.8, 148.4, 140.5, 132.9, 127.8, 126.9, 126.3, 119.2, 119.0, 114.6, 111.8, 102.3, 49.6, 42.5, 40.6, 27.8, 20.7; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₅N₆0₂S₂, 469.1480; found 469.1461; HPLC: method A R_t = 6.38, method B R_t = 7.18.

8- (6-(furan-2-yl)benzo [d] [1,3] dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H-purin-6- amine [DZ3-4]. 2-Furanylboronic acid (9.8 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 3.5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 12.5:1) to give 19.1 mg (72%) of DZ3-4. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 8.18 (s, 1H), 7.49 (d, J = 1.8 Hz, 1H), 7.25 (s, 1H), 6.96 (s, 1H), 6.71 (d, J= 3.3 Hz, 1H), 6.47 (dd, J= 1.8, 3.2 Hz, 1H), 6.06 (s, 2H), 4.20 (t, J= 7.0 Hz, 2H), 2.87 (m, 1H), 2.61 (t, J= 6.9 Hz, 2H), 2.00 (m, 2H), 1.14 (d, J= 6.3 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<¾ δ 154.3, 152.2, 151.2,

150.9, 149.5, 148.14, 148.08, 142.4, 129.0, 119.2, 117.5, 114.5, 111.5, 110.0, 109.0, 102.3, 42.8, 41.0, 28.5, 21.2; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₅N₆0₃S, 453.1709; found 453.1705; HPLC: method A R_t = 6.23, method B R_t = 6.82.

9- (3-(isopropylamino)propyl)-8-(6-(4-methoxyphenyl)benzo [d] [1,3] dioxol-5-ylthio)-9H- purin-6-amine [DZ3-3], 4-Methoxyphenylboronic acid (13.3 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 12.5:1) to give 20.5 mg (71%) of DZ3-3. 1H NMR (500 MHz, CDC1₃) δ 8.25 (s, 1H), 7.19 (d, J= 8.7 Hz, 2H), 6.95 (s, 1H), 6.86 (d, J= 8.7 Hz, 2H), 6.82 (s, 1H), 6.00 (s, 2H), 5.92 (br s, 2H), 4.01 (t, J= 6.7 Hz, 2H), 3.82 (s, 3H), 2.75 (septet, J= 6.3 Hz, lH), 2.43 (t, J = 6.7 Hz, 2H), 1.85 (m, 2H), 1.07 (d, J= 6.3 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 159.2, 154.3, 152.5, 151.5, 148.6, 147.8, 147.5, 139.0, 132.5, 130.4, 120.6, 119.8, 113.5, 113.0, 111.0, 101.8, 55.3, 49.1, 43.2, 40.9, 29.2, 22.2; HRMS (ESI) m/z [M+H]⁺ calcd. for

C₂₅H₂₉N₆0₃S, 493.2022; found 493.2010; HPLC: method A R, = 6.57, method B R_t = 7.55.

9-(3-(isopropylamino)propyl)-8-(6-(pyridin-4-yl)benzo[d][l,3]dioxol-5-ylthio)-9H-puri^ 6-amine [DZ3-5]. 4-Pyridinylboronic acid (10.8 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 3.5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 12.5:1) to give 14.8 mg (55%) of DZ3-5. 1H NMR (500 MHz, CDCl₃/MeOH-c¾ δ 8.53 (dd, J= 1.6, 4.8 Hz, 2H), 8.19 (s, 1H), 7.25 (dd, J= 1.6, 4.5 Hz, 2H), 7.11 (s, 1H), 6.89 (s, 1H), 6.11 (s, 2H), 4.07 (t, J= 6.8 Hz, 2H), 2.82 (m, 1H), 2.51 (t, J= 6.8 Hz, 2H), 1.91 (m, 2H), 1.13 (d, J= 6.3 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-i¾ δ 154.1, 152.0, 151.2, 150.0, 148.90, 148.85, 148.69, 137.9, 124.5, 119.1, 117.7, 115.3, 110.6, 102.5, 49.2, 42.8, 40.7, 28.4, 21.2; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₆N₇0₂S, 464.1869; found 464.1848; HPLC:

method A R_t = 5.13, method B R_t = 2.57.

8-(6-(4-bromophenyl)benzo[d|[l,3]dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine [DZ3-6]. 4-Bromophenylboronic acid. (17.6 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90° C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC

(hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:5:2:1) to give 13.9 mg (44%) of DZ3-6. 1H NMR (500 MHz, CDC1₃) δ 8.23 (s, 1H), 7.44 (d, J= 8.2 Hz, 2H), 7.13 (d, J= 8.2 Hz, 2H), 7.01 (s, 1H), 6.81 (s, 1H), 6.05 (s, 2H), 5.69 (br s, 2H), 4.08 (t, J= 6.0 Hz, 2H), 2.91 (m, 1H), 2.50 (t, J= 5.9 Hz, 2H), 1.98 (m, 2H), 1.20 (d, J= 6.4 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<¾ δ 154.2, 152.0, 151.2, 149.8, 149.1, 148.2, 139.8, 139.1, 131.2, 131.0, 122.0, 119.0, 117.9, 115.0, 111.1, 102.4, 50.1, 42.1, 40.2, 27.3, 20.2; HRMS (ESI) m/z

[M+H]⁺ calcd. for C₂₄H₂₆BrN₆0₂S, 541.1021/ 543.1001; found 541.1016/543.1004; HPLC: method A R_t = 6.93, method B R_t = 8.30.

8-(6-(furan-3-yl)benzo [d] [1,3] dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H-purin-6- amine [DZ3-27]. 3-Furanylboronic acid (9.8 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7 ), 20:1) to give 23.9 mg (90%) of DZ3-27. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 8.17 (s, 1H), 7.51 (s, 1H), 7.44 (s, 1H), 7.07 (s, 1H), 6.97 (s, 1H), 6.49 (s, 1H), 6.08 (s, 2H), 4.17 (t, J= 6.9 Hz, 2H), 2.93 (septet, J= 6.4 Hz, 1H), 2.64 (t, J= 7.1 Hz, 2H), 2.02 (m, 2H), 1.17 (d, J= 6.4 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<^) δ 154.1, 151.8, 151.0, 149.7, 149.0, 147.8, 142.6, 140.4, 131.7, 124.2, 118.9, 117.8, 114.8, 111.5, 110.7, 102.1, 49.2, 42.6, 40.6, 28.0, 20.7; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₅N₆0₃S, 453.1709; found 453.1711; HPLC: method A R_t = 6.18, method B R_t = 6.67.

5-(6-(6-amino-9-(3-(isopropylamino)propyI)-9H-purin-8-ylthio)benzo [d] [1,3] dioxol-5- yl)furan-2-carbaldehyde [DZ3-33]. 2-Formyl-5-furanylboronic acid (12.3 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 9.5 mg (34%) of DZ3-33. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 9.57 (s, 1H), 8.18 (s, 1H), 7.36 (d, J= 3.8 Hz, 1H), 7.32 (s, 1H), 7.07 (s, 1H), 6.98 (d, J= 3.8 Hz, 1H), 6.12 (s, 2H), 4.32 (t, J= 6.7 Hz, 2H), 3.31 (septet, J= 6.6 Hz, 1H), 2.93 (t, J= 6.8 Hz, 2H), 2.30 (m, 2H), 1.42 (d, J= 6.6 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<¾) δ 177.5, 156.8, 154.5, 152.2, 151.9, 151.4, 150.1, 149.7, 148.1 , 127.5, 124.3, 1 19.2, 1 18.7, 1 15.7, 1 12.7, 109.9, 102.9, 51.3, 41.8, 40.4, 26.4, 19.2; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₅N₆0₄S, 481.1658; found 481.1657; HPLC:

method A R_t = 5.87, method B R_t = 5.93.

8-(6-(lH-pyrrol-2-yl)benzo [d] [1,3] dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine [DZ3-29]. 1 -N-Boc-pyrrole-2-boronic acid (18.5 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. DMF was removed under reduced pressure and to the resulting residue was added CH₂C1₂ (1.5 mL) and TFA (0.3 mL). The mixture was stirred for 5 h at rt, then solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:9:2: 1) to give 5.8 mg (22%) of DZ3-29. 1H NMR (500 MHz, CDCl₃/MeOH-<¾) δ 8.17 (s, 1H), 7.04 (s, 1H), 7.01 (s, 1H), 6.88 (m, 1H), 6.27 (m, 1H), 6.21 (m, 1H), 6.03 (s, 2H), 4.17 (t, 7= 6.8 Hz, 2H), 3.29 (septet, J= 6.6 Hz, 1H), 2.80 (t, J= 6.7 Hz, 2H), 2.15 (m, 2H), 1.41 (d, J= 6.6 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₆N₇0₂S, 452.1869; found 452.1872; HPLC: method A ¾ = 6.13, method B R_t = 6.43.

8-(6-(lH-pyrazol-4-yl)benzo [d] [1,3] dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine [DZ3-30]. l-Boc-pyrazole-4-boronic acid pinacol ester (25.8 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then ¾0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 13.7 mg (53%) of DZ3-30. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 8.17 (s, IH), 7.58 (s, 2H), 7.10 (s, IH), 6.95 (s, IH), 6.06 (s, 2H), 4.08 (t, J= 6.9 Hz, 2H), 2.89 (septet, J= 6.4 Hz, IH), 2.57 (t, J= 7.2 Hz, 2H), 1.91 (m, 2H), 1.14 (d, J = 6.4 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<¾ δ 154.0, 152.1, 151.2, 149.7, 148.9, 147.5, 133.6, 132.0, 119.8, 119.1, 117.9, 115.3, 110.9, 102.1, 49.2, 42.9, 40.8, 28.3, 21.3; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂iH₂₅N₈0₂S, 453.1821; found 453.1819; HPLC: method A R_t = 5.60, method B R_t = 4.87.

9-(3-(isopropylamino)propyI)-8-(6-(5-methylfuran-2-yl)benzo[d] [l,3]dioxol-5-ylthio)- 9H-purin-6-amine [DZ3-35] . 4,4,5 , 5-Tetramethyl-2-(5-methyl-furan-2 -yl)- (l,3,2)dioxaborolane (21.9 mg, 0.1053 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:9:2:1) to give 11.5 mg (42%) of DZ3-35. 1H NMR (500 MHz, CDC1₃) δ 8.25 (s, IH), 7.19 (s, IH), 6.84 (s, IH), 6.63 (d, J= 3.1 Hz, IH), 6.07 (d, J= 2.5 Hz, IH), 5.98 (s, 2H), 5.93 (br s, 2H), 4.22 (t, J= 6.6 Hz, 2H), 2.94 (m, IH), 2.59 (t, J= 6.6 Hz, 2H), 2.34 (s, 3H), 2.05 (m, 2H), 1.20 (d, J= 6.3 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₇N₆0₃S, 467.1865; found 467.1869; HPLC: method A R_t = 6.49, method B R_t = 7.53.

2-(6-(6-amino-9-(3-(isopropylamino)propyl)-9H-purin-8-ylthio)benzo [d j [1,3] dioxol-5- yl)acetonitrile [DZ3-39].,4-Isoxazoleboronic acid pinacol ester (20.5 mg, 0.1053 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC

(hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2: 1 :0.5) to give 10.3 mg (%) of DZ3-39. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 8.17 (s, 1H), 7.14 (s, lH), 7.12 (s, lH), 6.10 (s, 2H), 4.35 (t, J= 6.9 Hz, 2H), 3.99 (s, 2H), 3.08 (septet, J= 6.5 Hz, 1H), 2.82 (t, J= 7.0 Hz, 2H), 2.25 (m, 2H), 1.27 (d, J= 6.5 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<¾) δ 154.2, 152.1, 152.0, 151.5, 150.8, 148.6, 147.7, 129.5, 119.2, 117.6, 116.2, 110.3, 102.7, 49.8, 42.5, 40.7, 27.7, 22.9, 20.4; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂oH₂₄N₇0₂S, 426.1712; found 426.1712; HPLC: method A R_t = 5.69, method B R_t = 4.57.

8- (6-(lH-pyrrol-3-yl)benzo [d] [1,3] dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine [DZ3-41]. l-Boc-pyrrole-3-boronic acid pinacol ester (30.9 mg, 0.1053 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 7.9 mg (30%) of DZ3-41. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 8.16 (s, 1H), 7.02 (s, 1H), 6.99 (s, 1H), 6.86 (m, 1H), 6.76 (m, 1H), 6.21 (m, 1H), 6.02 (s, 2H), 4.07 (t, J= 6.9 Hz, 2H), 2.95 (septet, J= 6.4 Hz, lH), 2.59 (t, J= 7.1 Hz, 2H), 1.96 (m, 2H), 1.19 (d, J= 6.4 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-^) δ 154.4, 152.1, 152.0, 151.0, 149.4, 146.7, 135.9, 131.6, 122.1, 119.1, 117.8, 117.5, 114.6, 111.0, 109.2, 101.9, 53.6, 42.6, 40.6, 27.8, 20.6; HRMS (ESI) m/z

[M+H]⁺ calcd. for C₂₂H₂₆N₇0₂S, 452.1869; found 452.1862; HPLC: method A R_t = 6.02, method B R_t = 6.27.

9- (3-(isopropylamino)propyl)-8-(6-(l-methyl-lH-pyrazol-5-yl)benzo[d| [l,3]dioxol-5- ylthio)-9H-purin-6-amine [DZ3-44], 1 -Methyl- l-H-pyrazole-5-boronic acid pinacol ester (18.2 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 2 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 20.1 mg (74%) of DZ3-44. 1H NMR (500 MHz, CDC1₃) δ 8.25 (s, 1H), 7.40 (d, J= 1.8 Hz, lH), 7.05 (s, 1H), 6.80 (s, lH), 6.08 (s, 2H), 6.06 (d, J= 1.8 Hz, 1H), 5.91 (br s, 2H), 4.10 (t, J= 6.7 Hz, 2H), 3.67 (s, 3H), 2.85 (septet, J= 6.3 Hz, 1H), 2.53 (t, J= 6.7 Hz, 2H), 1.97 (m, 2H), 1.13 (d, J= 6.3 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 154.4, 152.7, 151.7, 149.3, 149.0, 147.0, 140.6, 138.5, 127.7, 123.3, 120.0, 113.8, 111.7, 107.4, 102.5, 49.6, 43.2, 41.1, 37.1, 29.1, 22.0; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₇N₈0₂S, 467.1978; found 467.1985; HPLC: method A R_t = 5.74, method B Rt = 5.20.

8- (6-(lH-pyrazol-3-yI)benzo [d] [1,3] dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine [DZ3-46]. lH-pyrazole-3-boronic acid (33 mg, 0.293 mmol) was added to PU-H71 (50 mg, 0.0975 mmol) and NaHC0₃ (24.6 mg, 0.293 mmol). DMF (2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.2 mL) and Pd(PPh₃)₂Cl₂ (7 mg, 0.0098 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC

(hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 3.7 mg (8%) of DZ3-46. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 8.17 (s, 1H), 7.59 (d, J= 2.0 Hz, 1H), 7.10 (s, 1H), 7.07 (s, 1H), 6.38 (d, J= 2.0 Hz, 1H), 6.08 (s, 2H), 4.19 (t, J= 6.8 Hz, 2H), 3.31 (septet, J= 6.6 Hz, 1H), 2.89 (t, J= 7.2 Hz, 2H), 2.10 (m, 2H), 1.39 (d, J= 6.6 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-i¾ δ 154.5, 152.2, 152.1, 150.7, 149.5, 148.5, 148.3, 119.3, 119.1, 114.6, 114.5, 111.0, 110.9, 106.1, 102.3, 51.1, 41.7, 40.3, 26.0, 18.7; HRMS (ESI) m/z

[M+H]⁺ calcd. for C₂iH₂₅N₈0₂S, 453.1821; found 453.1826; HPLC: method A R_t = 5.65, method B R_t = 4.83.

9- (3-(isopropylamino)propyl)-8-(6-(isoxazol-4-yl)benzo [d | [1,3] dioxol-5-ylthio)-9H- purin-6-amine [DZ3-49]. 4-Isoxazoleboronic acid pinacol ester (20.5 mg, 0.1053 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.01 17 mmol) were added and the reaction mixture was heated under nitrogen at 60°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was attempted to be purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:7:2: 1) to give 7.4 mg (28%) of an inseparable mixture of DZ3-49 and DZ3-39 in a ratio of approximately 71 :29, respectively, as determined by HPLC. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 8.66 (s, 1H), 8.45 (s, 1H), 8.17 (s, 1H), 7.18 (s, 1H), 7.01 (s, 1H), 6.13 (s, 2H), 4.37 (t, J= 7.0 Hz, 2H), 3.27 (septet, J= 7.1 Hz, 1H), 2.89 (t, J= 7.1 Hz, 2H), 2.22 (m, 2H), 1.38 (d, J= 6.5 Hz, 6H); MS (ESI) m/z [M+H]⁺ 454.1 ; HPLC: method AR_t = 5.67 (DZ3-39, 29%) and 5.87 (DZ3-49, 71%); method B R_t = 4.58 (DZ3-39, 34%) and 5.57 (DZ3-49, 66%).

4-(6-(6-amino-9-(3-(isopropylamino)propyl)-9H-purin-8-ylthio)benzo[d] [l,3]dioxol-5- yl)benzaldehyde [DZ3-50]. 4-Formylphenylboronic acid (13 mg, 0.0877 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC

(hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2: 1 :0.5) to give 18.8 mg (66%) of DZ3-50. 1H NMR (500 MHz, MeOH-<¾ 5 10.01 (s, 1H), 8.15 (s, 1H), 7.85 (d, J = 8.1 Hz, 2H), 7.47 (d, J = 8.1 Hz, 2H), 7.14 (s, 1H), 6.93 (s, 1H), 6.13 (s, 2H), 4.05 (t, J= 6.8 Hz, 2H), 2.99 (septet, J = 6.4 Hz, 1H), 2.62 (t, J= 6.9 Hz, 2H), 1.99 (m, 2H), 1.24 (d, J = 6.4 Hz, 6H); ¹³C NMR (125 MHz, MeOH-£¾ δ 192.3, 154.1 , 151.9, 151.0, 149.8, 148.8, 148.5, 146.4, 139.5, 135.3, 130.0, 129.4, 119.0, 117.7, 1 15.0, 1 10.8, 102.4, 49.9, 42.2, 40.3, 27.4, 20.2; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₅H₂₇N₆0₃S, 491.1865; found 491.1877; HPLC: method A R_t = 6.23, method B R_t = 6.83. teri-Butyl 6-(3-(6-amino-8-(6-(3,5-bis(trifluoromethyl)phenyl)benzo [d] [1,3] dioxol-5- ylthio)-9H-purin-9-yl)propylamino)hexylcarbamate [TT-V-43 A] .

Bis(trifluoromethyl)phenylboronic acid (22.7 mg, 0.0878 mmol) was added to PU-H71-C6- linker (39.2 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 3 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:MeOH- NH₃ (7N), 10:1) to give 27.7 mg (63%) of TT-V-43 A. 1H NMR (500 MHz, CDC1₃) δ 8.17 (s, 1H), 7.78 (s, 3H), 7.14 (s, 1H), 6.89 (s, 1H), 6.12 (s, 2H), 5.80 (br s, 2H), 4.68 (br s, 1H), 4.16 (t, J= 6.3 Hz, 2H), 3.10 (m, 2H), 2.81 (t, J= 7.7 Hz, 2H), 2.67 (t, J= 6.2 Hz, 2H), 2.21 (m, 2H), 1.81 (m, 2H), 1.30-1.53 (m, 15H); HRMS (ESI) m/z [M+H]⁺ calcd. for

C₃₄H₄₀F₆N₇O₄S, 756.2767; found 1 '56.2753; HPLC: method A R_t = 8.17, method B R_t = 11.00.

N¹-(3-(6-amino-8-(6-(3,5-bis(trinuoromethyl)phenyl)benzo[d][l,3]dioxol-5-ylthio)-9H- purin-9-yl)propyl)hexane-l,6-diamine [TT-V-47B]. TT-V-43 A (26 mg, 0.0344 mmol) was dissolved in CH₂C1₂ (1.2 mL) and TFA (0.3 mL) was added and stirred at rt for 45 min. Then solvent was removed under reduced pressure and residue dried under high vacuum for 2 h to give TT-V-47B. Purified by preparatory TLC (hexane:CH₂Cl₂:MeOH-NH₃ (7N), 7:1) to give mg (%) of TT-V-47B. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 8.04 (s, 1H), 7.67 (s, 1H), 7.64 (s, 2H), 7.12 (s, 1H), 6.84 (s, 1H), 6.04 (s, 2H), 3.97 (t, J= 6.7 Hz, 2H), 2.85 (t, J= 7.0 Hz, 2H), 2.84 (t, J= 6.9 Hz, 2H), 2.77 (t, J= 7.0 Hz, 2H), 2.06 (m, 2H), 1.71 (m, 2H), 1.63 (m, 2H), 1.28 (m, 4H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₉H₃₂F₆N₇0₂S, 656.2242; found 656.2242; HPLC: method A R_t = 6.98, method B R, = 8.38.

PU-DZ13

Reagents and conditions: (a) RB(OH)₂, PdCl₂(PPh₃)₂, NaHC0₃, H₂0, DMF, 90°C. Scheme 4. Suzuki coupling of PU-DZ13.

2-fluoro-8-((6-(furan-2-yl)benzo [d | [1,3] dioxol-5-yl)methyl)-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine [DZ3-25]. 2-Furanylboronic acid (9.8 mg, 0.0877 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20: 1) to give 19.1 mg (72%) of DZ3-25. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ 6 7.46 (d, J= 1.4 Hz, 1H), 7.08 (s, 1H), 6.64 (s, 1H), 6.46 (dd, J = 1.4, 3.2 Hz, 1H), 6.34 (d, J= 3.2 Hz, 1H), 6.00 (s, 2H), 4.34 (s, 2H), 4.08 (t, J= 6.3 Hz, 2H), 2.85 (t, J= 6.3 Hz, 2H), 2.38 (d, J= 6.8 Hz, 2H), 1.70 (m, lH), 0.88 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH- d₄) 6 158.9 (d J= 208.5 Hz), 156.2 (d, J= 20.2 Hz), 152.6, 152.3 (d, J= 18.3 Hz), 151.9, 147.9, 147.1, 142.1, 126.1, 124.3, 115.8, 111.3, 110.0, 108.7, 108.2, 101.6, 57.0, 48.1, 42.3, 32.0, 27.7, 20.2; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₆FN₆0₃, 453.2050; found 453.2041; HPLC: method A^'¾ = 7.10, method B R_t = 8.52. 2-fluoro-8-((6-(furan-3-yI)benzo[d|[l,3]dioxoI-5-yI)methyI)-9-(2-(isobutyIamino)ethyI)- 9H-purin-6-amine [DZ3-26]. 3-Furanylboronic acid (9.8 mg, 0.0877 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 23.4 mg (88%) of DZ3-26. 1H NMR (500 MHz, CDC1₃) δ 7.49 (m, 1H), 7.46 (m, 1H), 6.82 (s, 1H), 6.63 (s, 1H), 6.46 (m, 1H), 5.96 (s, 2H), 5.70 (br s, 2H), 4.22 (s, 2H), 3.91 (t, J= 6.1 Hz, 2H), 2.75 (t, J= 6.1 Hz, 2H), 2.29 (d, J= 6.5 Hz, 2H), 1.70 (m, 1H), 0.83 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 158.9 (d, J= 208.5 Hz), 156.2 (d, J = 20.3 Hz), 152.7 (d, J= 18.2 Hz), 152.2, 147.5, 146.9, 143.1, 140.1, 127.0, 125.7, 124.7, 116.6, 111.8, 110.2, 109.4, 101.4, 57.5, 48.5, 43.0, 31.9, 28.1, 20.4; HRMS (ESI) m/z

[M+H]⁺ calcd. for C₂₃H₂₆FN₆0₃, 453.2050; found 453.2044; HPLC: method A R_t = 7.10, method B R, = 8.50.

8-((6-(lH-pyrroI-2-yI)benzo[d][l,3]dioxoI-5-yI)methyI)-2-fluoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine [DZ3-31]. l-N-Boc-pyrrole-2-boronic acid (18.5 mg, 0.0877 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. DMF was removed under reduced pressure and to the resulting residue was added CH₂C1₂ (1.5 mL) and TFA (0.3 mL). The mixture was stirred for 5 h at rt, then solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC

(hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:15:2:1) to give 5.2 mg (20%) of DZ3-31. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 6.91 (s, 1H), 6.84 (dd, J= 1.4, 2.5 Hz, 1H), 6.65 (s, 1H), 6.22 (m, 1H), 6.08 (dd, J= 1.4, 3.3 Hz, 1H), 5.97 (s, 2H), 4.26 (s, 2H), 4.06 (t, J= 6.7 Hz, 2H), 2.80 (t, J= 6.7 Hz, 2H), 2.55 (d, J= 7.0 Hz, 2H), 1.88 (m, 1H), 0.96 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-^) δ 158.8 (d, J= 208.1 Hz), 156.4 (d, J- 20.0 Hz), 152.9, 152.3 (d, J= 21.2 Hz), 147.4, 129.8, 127.9, 126.7, 118.5, 110.5, 109.3, 108.7, 108.6, 101.5, 56.6, 47.4, 41.1, 31.6, 27.1, 20.2; HRMS (ESI) m/z [M+H]⁺ calcd. for

C₂₃H₂₇FN₇0₂, 452,2210; found 452.2212; HPLC: method A R_t = 7.02, method B R_t = 8.30.

5-(6-((6-amino-2-fluoro-9-(2-(isobutylamino)ethyl)-9H-purin-8- yl)methyl)benzo[d] [l,3]dioxol-5-yl)furan-2-carbaldehyde [DZ3-34]. 2-Formyl-5- furanylboronic acid (12.3 mg, 0.0877 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 2.0 mg (7%) of DZ3-34. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 9.47 (s, 1H), 7.27 (d, J= 3.7 Hz, 1H), 7.20 (s, 1H), 6.77 (s, 1H), 6.63 (d, J= 3.7 Hz, 1H), 6.06 (s, 2H), 4.45 (s, 2H), 4.32 (t, J= 6.3 Hz, 2H), 2.83 (t, J= 6.3 Hz, 2H), 2.54 (d, J= 6.8 Hz, 2H), 1.83 (m, 1H), 0.93 (d, J = 6.7 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₄H₂₆FN₆0₄, 481.2000; found 481.1984; HPLC: method A R_t = 6.61, method B R_t = 7.62.

8-((6-(lH-pyrazol-4-yl)benzo[d|[l,3]dioxol-5-yl)methyl)-2-nuoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine [DZ3-32]. l-Boc-pyrazole-4-boronic acid pinacol ester (25.8 mg, 0.0877 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.5 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.15 mL) and Pd(PPh₃)₂Cl₂ (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 10.6 mg (40%) of DZ3-32. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 7.50 (s, 2H), 6.83 (s, lH), 6.69 (s, 1H), 5.98 (s, 2H), 4.18 (s, 2H), 4.01 (t, J= 6.6 Hz, 2H), 2.78 (t, J= 6.6 Hz, 2H), 2.40 (d, J= 7.0 Hz, 2H), 1.71 (m, 1H), 0.88 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<¾ δ 158.6 (d, J= 208.3 Hz), 156.3 (d, J= 19.6 Hz), 152.3, 152.1 (d, J= 21.2 Hz), 147.3, 147.1, 126.4, 126.2, 120.1, 115.8, 110.7, 109.9, 101.4, 56.3, 47.3, 40.8, 31.9, 27.0, 20.0; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₆FN₈0₂, 453.2163; found 453.2162; HPLC: method A R_t = 6.23, method B R_t = 6.55.

2-fluoro-9-(2-(isobutylamino)ethyI)-8-((6-(5-methyIfuran-2-yI)benzo[d|[l,3]dioxoI-5- yI)methyl)-9H-purin-6-amine [DZ3-36] . 4,4,5,5-Tetramethyl-2-(5-methyl-furan-2-yl)- (l,3,2)dioxaborolane (21.9 mg, 0.1053 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:15:2:1) to give 15.6 mg (57%) of DZ3-36. 1H NMR (500 MHz, CDC1₃) δ 7.04 (s, 1H), 6.53 (s, 1H), 6.26 (d, J= 3.1 Hz, IH), 6.21 (br s, 2H), 6.05 (d, J= 3.1 Hz, 1H), 5.94 (s, 2H), 4.37 (s, 2H), 3.98 (t, J= 6.3 Hz, 2H), 2.80 (t, J= 6.2 Hz, 2H), 2.34 (s, 3H), 2.31 (d, J= 6.8 Hz, 2H), 1.64 (m, 1H), 0.83 (d, J= 6.7 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₄H₂₈FN₆0₃, 467.2207; found 467.2200; HPLC: method A R_t = 7.29, method B R_t = 9.13.

8-((6-(lH-pyrrol-3-yl)benzo[d] [l,3]dioxoI-5-yI)methyI)-2-fluoro-9-(2- (isobutyIamino)ethyl)-9H-purin-6-ainine [DZ3-38]. l-Boc-pyrrole-3-boronic acid pinacol ester (30.9 mg, 0.1053 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ . (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.2 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h, then at 120°C for 6.5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:7:2:1) to give 20.9 mg (79%) of DZ3-38. 1H NMR (500 MHz, CDC1₃) δ 8.72 (br s, 1H), 6.85 (s, 1H), 6.83 (m, 1H), 6.76 (m, 1H), 6.62 (s, 1H), 6.34 (br s, 2H), 6.23 (m, 1H), 5.91 (s, 2H), 4.29 (s, 2H), 3.84 (t, J= 6.3 Hz, 2H), 2.67 (t, J= 6.3 Hz, 2H), 2.28 (d, J= 6.8 Hz, 2H), 1.60 (m, 1H), 0.82 (d, J= 6.6 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 158.8 (d, J= 208.0 Hz), 156.4 (d, J= 19.8 Hz), 153.0, 152.9 (d, J- 21.3 Hz), 146.82, 146.76, 130.0, 126.5, 123.3, 118.4, 116.9, 116.6, 110.6, 109.8, 109.3, 101.3, 57.6, 48.7, 43.0, 32.0, 28.3, 20.7; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₇FN₇0₂, 452.2210; found 452.2204; HPLC: method A R_t = 6.77, method B R_t = 7.93.

2-(6-((6-amino-2-fluoro-9-(2-(isobutylamino)ethyI)-9H-purin-8- yl)methyl)benzo[d][l,3]dioxol-5-yl)acetonitrile [DZ3-40]. 4-Isoxazoleboronic acid pinacol ester (20.5 mg, 0.1053 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:7:2:1) to give 8.3 mg (%) of DZ3-40. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 6.94 (s, 1H), 6.70 (s, 1H), 6.00 (s, 2H), 4.39 (t, J= 6.7 Hz, 2H), 4.31 (s, 2H), 3.84 (s, 2H), 3.18 (t, J= 6.6 Hz, 2H), 2.65 (d, J= 7.1 Hz, 2H), 1.94 (m, 1H), 0.99 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃/ MeOH-^) δ 158.7 (d, J= 210.4 Hz), 156.6 (d, J= 20.1 Hz), 152.1 (d, J= 18.2 Hz), 150.5, 148.1, 147.7, 126.7, 122.4, 117.9, 116.1, 110.6, 109.9, 101.8, 56.5, 47.4, 41.2, 31.3, 27.1, 21.5, 20.0;

HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₁H₂₅FN₇0₂, 426.2054; found 426.2048; HPLC:

method A R_t = 6.48, method B R_t = 7.10.

8-((6-(lH-pyrazol-3-yl)benzo[d| [l,3]dioxol-5-yl)methyl)-2-nuoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine [DZ3-43]. lH-pyrazole-3-boronic acid (11.8 mg, 0.1053 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4.5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7 ), 2:2:1:0.5) to give 3.5 mg (13%) of DZ3-43. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 7.63 (d, J= 2.15 Hz, 1H), 6.95 (s, 1H), 6.82 (s, 1H), 6.34 (d, J= 2.15 Hz, 1H), 6.01 (s, 2H), 4.35 (t, J= 6.8 Hz, 2H), 4.29 (s, 2H), 2.98 (t, J= 6.9 Hz, 2H), 2.64 (d, J= 7.0 Hz, 2H), 1.96 (m, lH), 0.99 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃/ MeOH-i¾ δ 158.8 (d, J= 208.7 Hz), 156.6 (d, J= 19.3 Hz), 152.4, 152.2 (d, J = 18.7 Hz), 148.3, 147.3, 127.1, 115.94, 115.93, 110.3, 110.1, 105.6, 101.8, 56.3, 47.4, 40.5, 31.5, 27.0, 20.2; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₆FN₈0₂, 453.2163; found

453.2149; HPLC: method A R_t = 6.37, method B R_t = 6.93.

2-nuoro-9-(2-(isobutylamino)ethyl)-8-((6-(l-methyl-lH-pyrazol-5- yl)benzo[d] [l,3]dioxol-5-yl)methyl)-9H-purin-6-amine [DZ3-45]. 1 -Methyl- 1-H-pyrazole- 5-boronic acidpinacol ester (18.2 mg, 0.0877 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) andNaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 2 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 4:7:2:1) to give 26.5 mg (97%) of DZ3-45. 1H NMR (500 MHz, CDC1₃) δ 7.49 (d, J= 1.5 Hz, lH), 6.76 (s, 1H), 6.74 (s, 1H), 6.28 (br s, 2H), 6.17 (d, J = 1.5 Hz, 1H), 6.01 (s, 2H), 3.99 (s, 2H), 3.90 (t, J= 6.3 Hz, 2H), 3.66 (s, 3H), 2.76 (t, J= 6.3 Hz, 2H), 2.31 (d, J= 6.8 Hz, 2H), 1.72 (m, 1H), 0.84 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 159.0 (d, J= 208.7 Hz), 156.4 (d, J= 20.0 Hz), 152.8 (d, J= 18.5 Hz), 151.5, 149.0, 147.1, 141.3, 138.8, 129.4, 123.4, 116.6, 110.7, 109.9, 106.9, 101.9, 57.8, 48.7, 43.1, 36.9, 31.8, 28.3, 20.6; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₈FN₈0₂, 467.2319; found 467.2323; HPLC: method A R_t = 6.37, method B R_t = 6.90. 2-nuoro-9-(2-(isobutylaimno)ethyl)^

yl)methyl)-9H-purin-6-amine [DZ3-48]. 2-Thienylboronic acid (11.2 mg, 0.0877 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 2 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC

(hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 18.3 mg (67%) of DZ3-48. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 7.31 (d, J= 5.0 Hz, 1H), 7.03 (dd, J= 3.1, 5.0 Hz, 1H), 6.92 (s, 1H), 6.87 (d, J= 3.0 Hz, 1H), 6.74 (s, 1H), 6.01 (s, 2H), 4.19 (s, 2H), 3.99 (t, J= 6.5 Hz, 2H), 2.78 (t, J= 6.5 Hz, 2H), 2.37 (d, J= 6.9 Hz, 2H), 1.70 (m, 1H), 0.88 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<¾ δ 158.7 (d, J= 209.4 Hz), 156.4 (d, J= 19.6 Hz), 152.21 (d, J= 18.4 Hz), 152.20, 148.1, 147.0, 141.5, 127.7, 127.3, 127.0, 126.0, 125.8, 115.9, 111.3, 110.0, 101.6, 57.1, 48.1, 42.2, 32.0, 27.8, 20.3; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₆FN₆0₂S, 469.1822; found 469.1830; HPLC: method A R_t = 7.21, method B R_t = 8.93.

2-fluoro-9-(2-(isobutylamino)ethyl)-8-((6-(isoxazol-4-yl)benzo[d][l,3]dioxol-5- yl)methyl)-9H-purin-6-amine [DZ3-51]. 4-Isoxazoleboronic acid pinacol ester (20.5 mg, 0.1053 mmol) was added to PU-DZ13 (30 mg, 0.0585 mmol) and NaHC0₃ (14.7 mg, 0.1755 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 60°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 2:2:1:0.5) to give 7.8 mg (29%) of an inseparable mixture of DZ3-51 and DZ3-40 in a ratio of approximately 44:56, respectively, as

determined by HPLC. MS (ESI) m/z [M+H]⁺ 454.4; HPLC: method A R_t = 6.46 (DZ3-40, 56%) and 6.65 (DZ3-51, 44%); method B R_t = 7.08 (DZ3-40, 65%) and 7.52 (DZ3-51, 35%).

PU-HZ151

Reagents and conditions: (a) RB(OH)₂, PdCl₂(PPh₃)₂, NaHC0₃, H₂0, DMF, 90°C. Scheme 5. Suzuki coupling of PU-HZ 151.

8-(6-(furan-2-yl)benzo [d] [1,3] dioxol-5-ylthio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine [TT5-53A]. 2-Furanylboronic acid (42.4 mg, 0.379 mmol) was added to PU-HZ151 (66.4 mg, 0.126 mmol) and NaHC0₃ (63.6 mg, 0.756 mmol). DMF (2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.4 mL) and Pd(PPh₃)₂Cl₂ (17.6 mg, 0.025 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 3.5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 25.2 mg (43%) of TT5-53A. 1H NMR (500 MHz, CDC1₃) δ 8.30 (s, 1H), 7.49 (d, J= 1.3 Hz, 1H), 7.17 (s, 1H), 6.84 (s, 1H), 6.75 (d, J- 3.3 Hz, 1H), 6.48 (dd, J= 1.8, 3.3 Hz, 1H), 5.97 (s, 2H), 5.89 (br s, 2H), 4.18 (t, J= 6.5 Hz, 2H), 2.86 (t, J= 6.5 Hz, 2H), 2.25 (s, 2H), 0.83 (s, 9H); ¹³C NMR (125 MHz, CDC1₃) δ 154.6, 153.0, 151.7, 151.1, 148.4, 147.9, 146.8, 142.2, 127.2, 120.8, 120.0, 112.8, 111.5, 110.0, 108.7, 101.9, 61.9, 49.6, 43.9, 31.5, 27.6; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₇N₆0₃S, 467.1865; found 467.1870; HPLC: method A R_t = 6.78, method B R_t = 7.83. 8- (6-(5-methylfuran-2-yl)benzo [d] [1,3] dioxol-5-ylthio)-9-(2-(neopentylamino)ethyl)-9H- purin-6-amine [DZ3-56]. 4,4,5,5-Te1xamethyl-2-(5-methyl-furan-2-yl)-(l,3,2)dioxaborolane (17.7 mg, 0.0853 mmol) was added to PU-HZ151 (30 mg, 0.0569 mmol) and NaHC0₃ (14.3 mg, 0.1707 mmol). DMF (1,2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0113 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4.5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 15:1) to give 9.3 mg (34%) of DZ3-56. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 8.19 (s, 1H), 7.24 (s, 1H), 6.89 (s, 1H), 6.62 (d, J= 3.2 Hz, 1H), 6.05 (d, J= 3.2 Hz, 1H), 6.03 (s, 2H), 4.36 (t, J= 6.0 Hz, 2H), 3.04 (t, J= 6.0 Hz, 2H), 2.47 (s, 2H), 2.33 (s, 3H), 0.95 (s, 9H); ¹³C NMR (125 MHz, CDCl₃/MeOH-i¾ δ 154.5, 152.4, 152.3, 151.2, 149.4, 149.2, 148.5, 147.7, 129.1, 119.4, 117.4, 114.3, 111.2, 108.6, 107.8, 102.2, 61.4, 49.3, 43.2, 31.4, 27.7, 13.7; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₄H₂₉N₆0₃S,

481.2022; found 481.2002; HPLC: method A R_t = 6.89, method B R_t = 7.58.

9- (2-(neopentylamino)ethyl)-8-(6-(thiophen-2-yl)benzo [d | [1,3] dioxol-5-yIthio)-9H-purin- 6-amine [DZ3-58]. 2-Thiopheneboronic acid (10.9 mg, 0.0853 mmol) was added to PU- HZ151 (30 mg, 0.0569 mmol) and NaHC0₃ (14.3 mg, 0.1707 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0113 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 16.0 mg (58%) of DZ3-58. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 8.18 (s, 1H), 7.32 (dd, J= 1.6, 4.7 Hz, 1H), 6.98-7.03 (m, 4H), 6.07 (s, 2H), 4.29 (t, J= 5.4 Hz, 2H), 3.03 (t, J= 5.4 Hz, 2H), 2.48 (s, 2H), 0.97 (s, 9H); ¹³C NMR (125 MHz, CDCl₃/MeOH- d₄) δ 154.3, 152.0, 150.8, 149.2, 148.9, 148.3, 140.5, 132.5, 127.8, 127.0, 126.3, 120.1, 119.2, 114.4, 111.8, 102.2, 61.1, 49.0, 42.9, 31.2, 27.6; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₇N₆0₂S₂, 483.1637; found 483.1621 ; HPLC: method A R_t = 7.14, method B R_t = 7.73. 8-(6-(lH-pyrrol-3-yl)benzo[d][l^]dioxol-5-ylthio)-9-(2-(neopentylamino)ethyl)-9H- purin-6-araine [DZ3-59]. l-Boc-pyrrole-3-boronic acid inacol ester (25 mg, 0.0853 mmol) was added to PU-HZ151 (30 mg, 0.0569 mmol) and NaHC0₃ (14.3 mg, 0.1707 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0113 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH- NH₃ (7N), 20:1) to give 10.1 mg (38%) of DZ3-59. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 8.15 (s, 1H), 7.03 (s, 1H), 6.95 (s, 1H), 6.85 (m, 1H), 6.76 (m, 1H), 6.20 (dd, J= 1.7, 2.4 Hz, 1H), 6.01 (s, 2H), 4.24 (t, J= 5.7 Hz, 2H), 2.98 (t, J= 5.7 Hz, 2H), 2.50 (s, 2H), 0.98 (s, 9H); ¹³C NMR (125 MHz, CDCl₃/MeOH-^) δ 154.3, 152.1, 149.6, 149.3, 146.8, 135.7, 122.3, 119.3, 118.6, 117.9, 117.4, 114.5, 111.1, 109.4, 101.9, 61.0, 49.3, 42.7, 31.2, 27.7; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₈N₇0₂S, 466.2025; found 466.2016; HPLC: method A R_t = 6.86, method B R_t = 7.20.

8-(6-(furan-3-yl)benzo [d] [1 ,3] dioxol-5-ylthio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine [DZ3-60]. 3-Furanylboronic acid (9.5 mg, 0.0853 mmol) was added to PU-HZ151 (30 mg, 0.0569 mmol) and NaHC0₃ (14.3 mg, 0.1707 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0113 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 13.8 mg (52%) of DZ3-60. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 8.17 (s, 1H), 7.50 (s, 1H), 7.43 (d, J= 1.5 Hz, 1H), 7.02 (s, 1H), 6.94 (s, 1H), 6.49 (d, J= 1.5 Hz, 1H), 6.06 (s, 2H), 4.29 (t, J = 5.9 Hz, 2H), 3.02 (t, J= 5.8 Hz, 2H), 2.46 (s, 2H), 0.94 (s, 9H); ¹³C NMR (125 MHz, CDCl₃/MeOH-i¾) δ 154.3, 152.0, 151.1, 149.6, 149.3, 147.9, 142.8, 140.6, 131.5, 124.3, 119.3, 118.8, 114.7, 111.7, 110.9, 102.2, 61.4, 49.1, 43.0, 31.3, 27.6; HRMS (ESI) m/z

[M+H]⁺ calcd. for C₂₃H₂₇N₆0₃S, 467.1865; found 467.1845; HPLC: method A R_t = 6.65, method B R_t = 7.09. 8-(6-(lH-pyrazoI-3-yI)benzo [d] [1,3] dioxoI-5-yIthio)-9-(2-(neopentyIamino)ethyI)-9H- purin-6-amine [DZ3-_.61]. lH-Pyrazole-3-boronic acid (19 mg, 0.1707 mmol) was added to PU-HZ151 (30 mg, 0.0569 mmol) and NaHC0₃ (14.3 mg, 0.1707 mmol). DMF (1.2 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0113 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 4 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 15: 1) to give 6.5 mg (25%) of DZ3-61. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 8.18 (s, 1H), 7.58 (d, J= 2.0 Hz, 1H), 7.05 (s, 2H), 6.40 (d, J= 2.0 Hz, 1H), 6.06 (s, 2H), 4.36 (t, J = 6.0 Hz, 2H), 3.01 (t, J = 6.0 Hz, 2H), 2.51 (s, 2H), 0.97 (s, 9H); ¹³C NMR (125 MHz, CDCl₃/MeOH-<¾ δ 154.6, 152.3, 150.8, 149.4, 148.6, 148.5, 120.1, 119.2, 114.5, 110.9, 106.0, 102.3, 61.2, 49.1, 42.5, 31.1, 27.5; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₇N₈0₂S, 467.1978; found 467.1972; HPLC: method A R_t = 6.50, method B R_t = 6.61.

S6-4 S6-5 d, e d, e

S6-6

PU-WS4 n= 2, R= isopropyl

PU-WS9 n= 1 , R= isobutyl

PU-WS21 n= 1 , R= neopentyl

Reagents and conditions: (a) NaN0₂, KI, AcOH/TFA, 0°C; (b) 8-mercaptoadenine, Cs₂C0₃, PdCl₂(dppf), DMF, 80°C, 48h; (c) NIS, TFA, CH₃CN, rt, 2h ;(d) 1,3-dibromopropane or 1,2- dibromoethane, Cs₂C0₃, DMF, rt; (e) isopropylamine or isobutylamine or neopentylamine, DMF, rt; (f) DDQ, dioxane, 100°C.

Scheme 6. Synthesis of PU-WS4, PU-WS9 and PU-WS21.

6-iodo-2,3-dihydrobenzofuran (S6-2). A solution of 2,3-dihydrobenzofuran-6-amine (S6-1; 0.74 g, 5.5 mmol) in acetic acid (25 mL) and TFA (2 mL) was cooled in an ice bath for 5 minutes. NaN0₂ (0.454g, 6.6 mmol) was added in 3 portions followed by KI (2.73 g, 16.4 mmol). The resulting mixture was stirred at 0°C for 15 minutes and quenched with H₂0 (20 mL). The mixture was extracted with EtOAc (3 x 150 mL) and the organic layer was washed with NaS₂0₃, brine, dried over MgS0₄ and filtered. The filtrate was condensed under reduced pressure and the residue was purified by flash chromatography (hexane:EtOAc, 90:10 to 40:60) to yield S6-2 (0.82 g, 61%) as a pale-yellow solid. ^XH NMR (500 MHz, CDC1₃) δ 7.14 (d, J- 7.6 Hz, 1H), 7.11 (s, 1H), 6.89 (d, J= 7.6 Hz, 1H), 4.54 (t, J= 8.7 Hz, 2H), 3.14 (t, J= 8.7 Hz, 2H); ¹³C NMR (125 MHz, CDC1₃) δ 161.1, 129.4, 127.1, 126.4, 118.7, 91.7, 71.6, 29.4.

8-(2,3-dihydrobenzofuran-6-ylthio)-9H-purin-6-amine (S6-3). To a solution of S6-2 (50 mg, 0.2 mmol) in DMF (2 mL) was added 8-mercaptoadenine (34 mg, 0.2 mmol), Cs₂C0₃ (99.4 mg, 0.3 mmol) and PdCl₂(dppf) (33 mg, 0.02 mmol). The mixture was degassed for 5 minutes with argon and stirred at 80 °C under argon protection for 48 h. The resulting mixture was concentrated under reduced pressure and the residue was purified by flash chromatography (CH₂Cl₂:MeOH, 100:0 to 90:10) to yield S6-3 (25 mg, 44%) as a yellow solid. 1H NMR (500 MHz, CD₃OD) δ 8.14 (s, 1H), 7.24 (d, J= 7.6 Hz, 1H), 7.07 (d, J= 7.3 Hz, 1H), 6.97 (s, 1H), 4.62 (t, J= 8.7 Hz, 2H), 3.25 (t, J= 8.7 Hz, 2H); MS (ESI) m/z 285.8 [M+H]⁺; HRMS (ESI) m/z [M+H]⁺ calcd. for C₁₃H₁₂N₅OS, 286.0763; found 286.0768.

8-(5-iodo-2,3-dihydrobenzofuran-6-ylthio)-9H-purin-6-ainine (S6-4) To a solution of S6- 3 (40 mg, 0.14 mmol) in 6 mL of acetonitrile was added TFA (40 \iL) and NIS (63 mg, 0.28 mmol). The resulting mixture was stirred at rt for 2 h. The reaction mixture was concentrated under reduced pressure and the residue was purified by flash chromatography

(CH₂Cl₂:MeOH, 100:0 to 90:10) to afford S6-4 (48 mg, 53%) as a yellow gum. Ή NMR (500 MHz, CDC1₃) δ 8.26 (s, 1H), 7.79 (s, 1H), 7.12 (s, 1H), 4.65 (t, J= 8.8 Hz, 2H), 3.28 (t, J= 8.7 Hz, 2H); MS (ESI) m/z 412.0 (M+H)⁺.

8-(5-iodo-2,3-dihydro-benzofuran-6-ylsulfanyl)-9-(3-isopropylamino-propyl)-9jFi -purin- 6-ylamine (PU-WS4). A mixture of S6-4 (54 mg, 0.13 mmol), Cs₂C0₃ (127 mg, 0.39 mmol) and 1,3-dibromopropane (202 mg, 0.65 mmol) in anhydrous DMF (2 mL) was stirred at rt for 2 h. Solvent was removed under reduced pressure and the residue purified by

chromatography (CH₂Cl₂:MeOH:AcOH). The resulting solid was dissolved in DMF (2 mL) and isopropylamine (0.347 g, 0.5 mL, 5.9 mmol) was added and the solution stirred overnight at rt. The reaction afforded PU-WS4 (13 mg, 20%; over two-steps) as a yellow solid after purification. 1H NMR (500 MHz, CDC1₃/CD₃0D) δ 8.26 (s, 1H), 7.77 (s, 1H), 7.07 (s, 1H), 4.65 (t, J= 8.7 Hz, 2H), 4.47 (t, J= 6.9 Hz, 2H), 3.20-3.33 (m, 3H), 3.01 (t, J= 7.5 Hz, 2H), 2.33 (m, 2H), 1.34 (d, J= 6.5 Hz, 6H); MS (ESI) m/z 511.2 [M+H]⁺; HRMS (ESI) m/z

[M+H]⁺ calcd. for C₁₉H₂₄IN₆OS, 511.0777; found 511.0779.

S-iS^odo- ^-dihydro-benzofuran-e-ylsulfany^- -i -isobutylamino-ethy^-^-purin-e- ylamine (PU-WS9). A mixture of S6-4 (30 mg, 0.073 mmol), Cs₂C0₃ (71 mg, 0.22 mmol) and 1,2-dibromoethane (69 mg, 0.365 mmol) in anhydrous DMF (1 mL) was stirred at rt for 2 h. Solvent was removed under reduced pressure and the residue purified by chromatography (CH₂Cl₂:MeOH:AcOH). The resulting solid was dissolved in DMF (2 mL) and isobutylamine (0.241 g, 0.33 mL, 3.3 mmol) was added and the solution stirred overnight at rt. The reaction afforded PU-WS9 (15 mg, 40%; over two-steps) as a pale yellow solid. 1H NMR (500 MHz, CDCI3) δ 8.26 (s, 1H), 7.63 (s, 1H), 6.56 (s, 1H), 6.27 (br s, 2H), 4.57 (t, J= 8.5 Hz, 2H), 4.50 (t, J= 5.5 Hz, 2H), 3.20 (t, J= 8.5 Hz, 2H), 3.12 (t, J= 5.5 Hz, 2H), 2.59 (d, J= 7 Hz, 2H), 1.99 (m, 1H), 0.97 (d, J= 7 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for Ci₉H₂₄IN₆OS, 511.0777; found 511.0790.

8-((5-iodo-2,3-dihydrobenzofuran-6-yl)thio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine (PU-WS21). Following the procedure to make PU-WS9, compound PU-WS21 was obtained as a white solid. ¹HNMR (500 MHz, CDC1₃, δ): 7.56 (s, 1H), 6.60 (s, 1H), 4.47 (t, J = 8.7 Hz, 2H), 4.37 (m, 2H), 3.06-3.11 (m, 4H), 2.45 (s, 2H), 0.83 (s, 9H); HRMS (m/z): [M+H]⁺ calcd for C₂₀H₂₆IN₆OS 525.0933; found 525.0927.

PU-WS10

Reagents and conditions: (a) NIS, CH₃CN, 0°C, 20 min.; (b) 8-mercaptoadenine, neocuproine, Cul, NaOt-Bu, DMF, 110°C; (c)NaN0₂, KI, AcOH/TFA, 0°C; (d) 1,3-dibromopropane, Cs₂C0₃, DMF, rt; (e) isopropylamine, DMF, rt.

Scheme 7. Synthesis of PU-WS 10.

5-iodo-2,3-dihydrobenzofuran-6-amine (S7-1). To a solution of 2,3-dihydroberizofuran-6- amine (S6-1; 95 mg, 0.7 mmol) in acetonitrile (3 mL) cooled in an ice-bath was added NIS (158 mg, 0.7 mmol). After stirring at 0 °C for 20 min, the mixture was condensed and purified by flash chromatography (hexane:EtOAc, 90:10 to 20:80) to yield S7-1 (180 mg, 98%) as a yellow solid. 1H NMR (500 MHz, CDC1₃) δ 7.38 (s, 1H), 6.26 (s, 1H), 4.53 (t, J= 8.5 Hz, 2H), 3.09 (t, J= 8.4 Hz, 2H); MS (ESI) m/z 261.9 [M+H]⁺.

8-(6-amino-2,3-dihydrobenzofuran-5-ylthio)-9H-purin-6-amine (S7-2). The mixture of S7-1 (80 mg, 0.31 mmol), 8-mercaptoadenine (52 mg, 0.3 mmol), neocuproine (7 mg, 0.03 mmol), Cul (7 mg, 0.03 mmol) and sodium t-butoxide (100 mg, 1.04 mmol) was suspended in 10 mL of DMF and stirred at 110 °C overnight. The mixture was concentrated under reduced pressure and the residue purified by flash chromatography (hexane:EtOAc, 90:10 to 20:80) to yield S7-2 (50 mg, 56%) as a pale yellow solid. 1H NMR (500 MHz, CDC1₃) δ 8.15 (s, 1H), 7.33 (s, 1H), 6.35 (s, 1H), 4.57 (t, J= 8.5 Hz, 2H), 3.14 (t, J= 8.4 Hz, 2H); MS (ESI) m/z 301.0 [M+H]⁺.

8- (6-iodo-2,3-dihydrobenzofuran-5-ylthio)-9H-purin-6-amine (S7-3). To a solution of S7-2 (25 mg, 0.08 mmol) in acetic acid/TFA (5 mL/1 mL) cooled in ice-bath was added NaN0₂ (7 mg, O.lmmol) and KI (27 mg, 0.16 mmol). The mixture was stirred at 0°C for 10 minutes and condensed under reduced pressure. The residue was purified by flash

chromatography (CH₂Cl₂:MeOH, 100:0 to 90:10) to yield S7-3 (13 mg, 41%) as a yellow solid. 1H NMR (500 MHz, CD₃OD) δ 8.13 (s, 1H), 7.57 (s, 1H), 7.44 (s, 1H), 4.66 (t, J= 8.5 Hz, 2H), 3.22 (t, J= 8.6 Hz, 2H); MS (ESI) m/z 411.9 [M+H]⁺.

9- (3-bromopropyl)- 8-(6-iodo-2,3-dihydrobenzofuran-5-ylthio)-9H-purin-6-amine (S7- 4). To a solution of S7-3 (13 mg, 0.03 mmol) in DMF (2 mL) was added 1 ,3-dibromopropane (16 \iL, 0.16 mmol) and Cs₂C0₃ (20 mg, 0.06 mmol) and the resulting mixture was stirred at rt for 40 minutes. The mixture was condensed under reduced pressure and the residue was purified by flash chromatography to yield S7-4 (6.2 mg, 34%). 1H NMR (500 MHz, CDC1₃) δ 8.31 (s, 1H), 7.37 (s, 2H), 6.00 (br s, 2H), 4.62 (t, J= 8.7 Hz, 2H), 4.36 (t, J= 6.7 Hz, 2H), 3.42 (t, J= 6.2 Hz, 2H), 3.18 (t, J= 8.9 Hz, 2H), 2.39 (m, 2H); MS (ESI) m/z 531.9/533.9 [M+H]⁺.

8-(6-iodo-2,3-dihydro-benzofuran-5-ylsulfanyl)-9-(3-isobutylamino-ethyl)-9H-purin-6- ylamine (PU-WS10). A solution of S7-4 (6.2 mg, 0.012 mmol) and isopropylamine (0.2 mL) in DMF (1 mL) was stirred for 12 h. Solvent was removed under reduced pressure and the residue purified by preparative thin layer chromatography (CHCl₃:MeOH-NH₃ (7N), 20:1) to afford PU-WS10 (4.0 mg, 60%) as a pale yellow solid. 1H NMR (400 MHz, CDC1₃) δ 8.27 (s, 1H), 7.69 (s, 1H), 7.27 (s, 1H), 5.93 (br s, 2H), 4.66 (t, J= 8.8 Hz, 2H), 4.29 (t, J= 7 Hz, 2H), 3.33 (t, J= 8.7 Hz, 2H), 2.74 (septet, J= 6.2 Hz, 1H), 2.58 (t, J= 6.8 Hz, 2H), 1.98 (m, 2H), 1.05 (d, J= 6.5 Hz, 6H); ¹³C NMR (100 MHz, CDC1₃) δ 162.0, 154.4, 152.7, 151.9, 147.8, 141.7, 130.2, 128.6, 121.1, 120.0, 74.1, 71.7, 48.9, 44.0, 41.6, 30.9, 30.2; MS (ESI) m/z 511.1 [M+H]⁺.

PU-WS3 =F, X₃=CN, n=2, R= isopropyl

PU-DZ8 Xi=CH₂, X₂=F, n= 2, R= isopropyl i=CH₂, X₂

PU-WS5 X^S, X₂=H, X₃=CN, n= 2, R= isopropyl

PU-H71 X^S, X₂=H, n= 2, R= isopropyl

PU-WS6 X^S, X₂=H, X₃=CCf-Bu, n= 2, R= isopropyl

PU-HZ150 X^S, X₂=H, n= 1 , R= isobutyl

PU-WS7 X^S, X₂=H, X₃=CCPh, n= 2 R= isopropyl

PU-HZ151 X^S, X₂=H, n= 1 , R= neopentyl

PU-WS8 X-,=S, X₂=H, X₃=CCH, n= 2, R= isopropyl

PU-DZ13 X₁=CH₂, X₂=F, n= 1 , R= isobutyl

PU-WS16 X^S, X₂=H, X₃=CCH, n= 1 , R= isobutyl PU-WS19 X^S, X₂=H, X₃=CCH, n= 1 , R= neopentyl PU-WS20 X₁=CH₂, X₂=F, X₃=CCH, n= 1 , R= isobutyl

Reagents and conditions: (a) Boc₂0, Et₃N, THF, rt, 12h; (b) PdC½(dppf), Zn(CN)₂, Zn, DMF, 130°C; (c) 10% TFA-CH₂C1₂, rt, 2-5h; (d) Cul, PdCl₂(PPh₃)₂, i-butylacetylene or phenylacetylene or trimethylsilanylacetylene, Et₃N, DMF, 90°C, 24h; (e) KOH, MeOH, rt, 2h.

Scheme 8. Synthesis of PU-WS 3 , PU-WS 5 , PU-WS6, PU-WS7 and PU-WS8, PU-WS16, PU-WS19 and PU-WS20. e-te-aiiMiio-l-fluoro-P-iB-isopropylainiiio-propy^-Pii-purin-S-ylmethyl]- benzo[l,3]dixole-5-carbonitrile (PU-WS3). A solution of PU-DZ8 (118 mg, 0.231 mmol), (Boc) 0 (55 mg, 0.254 mmol) and triethylamine (16 mg, 0.231 mmol) in THF (2 mL) was stirred at room temperature for 12 h. Following solvent removal, the residue was purified by preparative thin layer chromatography (CHCl₃:MeOH-NH₃ (7N), 20:1) to afford Boc- protected PU-DZ8 (120 mg, 85%; MS (ESI) m/z 613.05 [M+H]⁺). To a solution of Boc- protected PU-DZ8 (26 mg, 0.04 mmol) in DMF (3 mL) was added PdCl₂(dppf) (17 mg, 0.02 mmol), Zn(CN)₂ (10 mg, 0.08 mmol) and Zn (3 mg, 0.04 mL) and the resulting mixture was stirred at 130°C overnight. The reaction mixture was condensed under reduced pressure and the residue was purified by flash chromatography (CHCl₃:MeOH-NH₃ (7N), 20:1) to yield Boc-protected PU-WS3 as a white solid. To a solution of this in 2 mL of CH C1₂ was added 0.2 mL of TFA and the mixture was stirred at room temperature for 5 h. The reaction mixture was condensed under reduced pressure and the residue purified by flash chromatography (CHCl₃:MeOH-NH₃ (7N), 20:1) to yield PU-WS3 as a white solid in quantitative yield (12 mg, 59% for three steps). 1H NMR (500 MHz, CD₃OD) δ 7.13 (s, 1H), 6.96 (s, 1H), 6.03 (s, 2H), 4.31 (s, 2H), 4.25 (t, J= 7 Hz, 2H), 3.26 (m, 1H), 3.01 (t, J= 7.5 Hz, 2H), 2.14 (m, 2H), 1.23 (d, J= 6.5 Hz, 6H); ¹³C NMR (125 MHz, CD₃OD) δ 160.4 (d, J= 208 Hz), 158.5 (d, J= 19 Hz), 153.8, 153.5 (d, J= 19 Hz), 151.7, 149.1, 137.2, 119.1, 117.4, 112.6, 112.4, 106.3, 104.4, 52.3, 43.4, 40.8, 33.2, 27.7, 19.3; MS (ESI) m/z 412.3 [M+H]⁺.

6-[6-amino-9-(3-isopropylamino-propyl)-9H-purin-8-ylsulfanyl]benzo[l,3]dixole-5- carbonitrile (PU-WS5). The procedure for the preparation of PU-WS3 was followed starting from PU-H71. The reaction afforded PU-WS5 (6.5 mg, 32% for three steps) as a white solid. 1H NMR (500 MHz, CDCl₃/CD₃OD) 5 8.21 (s, 1H), 7.22 (s, 1H), 7.19 (s, 1H), 6.19 (s, 2H), 4.40 (t, J= 7 Hz, 2H), 3.09 (septet, J= 6.5 Hz, 1H), 2.83 (t, J= 7.5 Hz, 2H), 2.26 (m, 2H), 1.25 (d, J= 6.5 Hz, 6H); MS (ESI) m/z 412.2 [M+H]⁺; HRMS (ESI) m/z [M+H]⁺ calcd. for C₁₉H₂₂N₇0₂S, 412.1556; found 412.1560.

8-[6-(3,3-dimethyl-but-l-ynyl)-benzo[l,3]dioxol-5-ylsulfanyl]9-(3-isopropylamino- propyl)-9H-purin-6-ylamine (PU-WS6). A solution of PU-H71 (70 mg, 0.137 rnmol), (Boc)₂0 (35 mg, 0.161 mmol) and triethylamine (13 mg, 0.137 rnmol) in THF (2 mL) was stirred at room temperature for 12 h. Following solvent removal, the residue was purified by preparative thin layer chromatography (CHCl₃:MeOH-NH₃ (7N), 20:1) to afford Boc- protected PU-H71 (74 mg, 88%; MS (ESI) m/z 612.89 [M+H]⁺). To a solution of Boc- protected PU-H71 (0.24 g, 0.39 mmol) in DMF (2 mL) was added Cul (4 mg, 0.1 mmol), PdCl₂(PPh₃)₂ (14 mg, 0.02 mmol), t-butylacetylene (72 μί, 0.59 mmol) and triethylamine (137 and the mixture was stirred at 90 °C for 24 h. The reaction mixture was condensed under reduced pressure and purified by chromatography to afford a solid. To a solution of the solid in 15 mL of CH₂C1₂ was added TFA (1.5 mL) and stirred at RT for 2 hrs. The mixture was condensed and purified by flash chromatography to afford PU-WS6 (97 mg, 52 % for three steps) as a white solid. 1H NMR (500 MHz, CDCl₃/CD₃OD) δ 8.25 (s, 1H), 6.99 (s, 1H), 6.97 (s, 1H), 6.05 (s, 2H), 4.41 (t, J= 7 Hz, 2H), 3.29 (m, 1H), 2.98 (t, J= 7.5 Hz, 2H), 2.24 (m, 2H), 1.34 (d, J= 6.5 Hz, 6H), 1.18 (s, 9H); ¹³C NMR (125 MHz, CDCI3/CD₃OD) δ 50.5, 41.3, 40.2, 30.3, 27.8, 25.9, 18.5; MS (ESI) m/z 467.3 [M+H]⁺; HRMS (ESI) m/z

[M+H]⁺ calcd. for C₂₄H₃iN₆0₂S, 467.2229; found 467.2233.

9-(3-isopropylamino-propyl)-8-(6-phenylethynyl-benzo[l,3]dixool-5-ylsulfanyl)-9i?- purin-6-ylamine (PU-WS7). The procedure for the preparation of PU-WS6 was followed with phenylacetylene (65 \iL, 0.59 mmol) to afford PU-WS7 (46 mg, 34% in three steps) as a white solid. 1H NMR (500 MHz, CDC1₃/CD₃0D) δ 8.2 (s, 1H), 7.30-7.40 (m, 5H), 7.08 (s, 1H), 6.96 (s, 1H), 6.06 (s, 2H), 4.27 (m, 2H), 2.69 (m, 1H), 2.51 (m, 2H), 1.97 (m, 2H), 1.01 (d, J= 6.5 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃/CD₃0D) δ 154.3, 152.3, 151.1, 148.8, 147.3, 131.3, 128.7, 128.3, 124.4, 122.4, 120.4, 119.3, 112.9, 112.4,102.3, 94.2, 86.6, 50.5, 43.1, 41.3, 29.2, 21.7; MS (ESI) m/z 487.2 [M+H]⁺; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₆H₂₇N₆0₂S, 487.1903; found 487.1913.

8-(6-ethynyl-benzo[l,3]dioxol-5-ylsulfanyl)-9-(3-isopropylamino-propyl)-9iy-purin-6- ylamine (PU-WS8). The procedure for the preparation of PU-WS6 was followed with trimethylsilanylacetylene (82 \LL, 0.59 mmol), and following coupling a white solid was obtained and used without further purification. To this was added MeOH (10 mL) and KOH (90 mg) and was stirred at rt for 2 hrs. The reaction mixture was concentrated under reduced pressure and to the resulting residue was added 2 mL of 10% TFA-CH₂C1₂ and was stirred at rt for 2 hrs. The mixture was concentrated under reduced pressure and the residue

chromatographed to afford PU-WS8 (5.2 mg, 26% for four steps) as a pale yellow solid. 1H NMR (500 MHz, CDC1₃) δ 8.32 (s, 1H), 6.99 (s, 1H), 6.84 (s, 1H), 6.00 (s, 2H), 5.61 (br s, 2H), 4.31 (t, J= 7 Hz, 2H), 3.31 (s, 1H), 2.71 (m, 1H), 2.56 (t, J = 7 Hz, 2H), 1.97 (m, 2H), 1.02 (d, J= 6.5 Hz, 6H); MS (ESI) m/z 411.2 [M+H]⁺; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₀H₂₃N₆O₂S, 411.1603 ; found 411.1605.

8- ((6-ethynylbenzo [d | [1,3] dioxol-5-yl)thio)-9-(2-(isobutylamino)ethyl)-9iy-purin-6- amine (PU-WS16). Following the procedure to make PU-WS8, PU-WS16 was obtained as a white solid. 1H NMR (500 MHz, CDC1₃) δ 8.24 (s, 1H), 6.91 (s, 1H), 6.77 (s, 1H), 6.01 (s, 2H), 5.78 (br s, 2H), 4.33 (t, J= 6.1 Hz, 2H), 3.24 (s, 1H), 2.92 (t, J- 6.1 Hz, 2H), 2.38 (d, J = 6.8 Hz, 2H), 1.63 (m, 1H), 0.77 (d, J= 6.6 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 155.1, 153.5, 151.9, 149.6, 148.5, 147.0, 128.0, 124.1, 117.0, 114.8, 1 1 1.3, 102.6, 82.9, 81.4, 57.9, 49.3, 44.2, 28.8, 28.4, 20.9; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₀H₂₂N₆O₂S, 411.1603; found 411.1606.

8-(6-ethynylbenzo [d] [1,3] dioxol-5-ylthio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine (PU-WS19). Following the procedure to make PU-WS8, PU-WS19 was obtained as a white solid. 1H NMR (500 MHz, CDC1₃) δ 8.32 (s, 1H), 6.97 (s, 1H), 6.83 (s, 1H), 5.98 (s, 2H), 5.76 (br s, 2H), 4.35 (m, 2H), 3.06 (s, 1H), 2.97 (m, 2H), 2.33 (s, 2H), 0.82 (s, 9H); ¹³C NMR (125 MHz, CDC1₃) δ 154.5, 152.9, 151.5, 149.1, 147.9, 146.5, 120.1, 117.7, 112.9, 111.9, 102.2, 82.3, 81.0, 61.9, 49.8, 43.9, 31.5, 27.7; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂iH₂₅N₆0₂S, 425.1760; found 425.1753.

8-((6-ethynylbenzo [d] [1,3] dioxol-5-yl)methyl)-2-fluoro-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine (PU-WS20). Following the procedure to make PU-WS8, PU-WS20 was obtained from PU-DZ13 as a white solid. 1H NMR (MeOH-i 500 MHz) δ: 6.98 (s, 1H), 6.70 (s, 1H), 6.02 (s, 2H), 4.36 (s, 2H), 4.20 (t, J = 6.4 Hz, 2H), 2.92 (t, J = 6.4 Hz, 2H), 2.42 (d, J = 6.9 Hz, 2H), 2.03 (s, 1H), 1.69 (m, 1H), 0.87 (d, J = 6.8 Hz, 6H); ¹³C NMR (MeOH- d4, 125 MHz) δ: 159.8, 158.1, 152.6, 151.4, 149.8, 147.2, 134.3, 1 16.2, 1 14.2, 112.5, 109.8, 102.3, 80.9, 57.5, 43.0, 32.6, 29.8, 28.2, 20.5.

PU-HT165 X=CH₂, R= isobutyi

S9-4 X=CH₂ PU-HT175 X=CH₂, R= neopentyl S9-5 X⁼CH2CH2 PU-RK11 X=CH₂CH₂, R= isopropyl

PU-RK12 X=CH₂CH₂, R= 1-imidazoyl

Reagents and conditions: (a) NaN0₂, 10% HCl, 0°C; KI, 0°C to rt; (b) 8-mercaptoadenine, neocuproine, Cul, NaOt-Bu, DMF, 110°C, 24 h; (c) NIS, TFA, CH₃CN, rt; (d) 1,2-dibromoethane or 1,3-dibromopropane, Cs₂C0₃, DMF, rt; (e) isobutylamine or neopentylamine or isopropylamine or imidazole, DMF, rt.

Scheme 9. Synthesis of PU-HT165, PU-HT175, PU-RK11, PU-RK12.

6-Iodo-2,3-dihydrobenzo[b][l,4]dioxine (S9-2). 2,3-dihydrobenzo[b][l,4]dioxin-6-amine (S9-1; 5 g, 33 mmol) was dissolved in 10% HCl solution and cooled to 0°C. Then, 30 mL of a cold aqueous solution of NaN0₂ (4.6 g, 66 mmol) was added over a period of 15 min and the reaction mixture was stirred at 0°C for an additional 10 min, followed by the addition of urea (1.6 g, 27 mmol). After 15 min, 40 mL of a suspension of KI (16.5 g, 100 mmol) in water/CH₂Cl₂ (1:1) was added. The reaction mixture was stirred overnight at room

temperature then extracted with CH₂C1₂, dried over MgS0 . And condensed under reduced pressure and the residue was purified by flash chromatography (hexane:EtOAc, 100:0 to 90:10) to afford S9-2 (7.4 g, 86%) as a colorless oil. 1H NMR (500 MHz, CDC1₃) δ 7.28 δ (s, 1H), 7.13 (d, J= 8.5 Hz, 1H), 6.63 (d, J= 8.5 Hz, 1H), 4.27-4.24 (m, 4H). 8- (7-Iodo-2,3-dihydrobenzo[b| [l,4]dioxin-6-ylthio)-9H-purin-6-amine (S9-3). To a solution of S9-2 (1.26 g, 4.8 mmol) in DMF (15 mL) was added 8-mercaptoadenine (0.400 g, 2.4 mmol), neocuproine (0.056 g, 0.24 mmol), Cul (0.044 g, 0.24 mmol) and NatOBu (0.460 g, 4.8 mmol). The reaction mixture was stirred at 110 °C for 24h. Solids were filtered and the filtrate was condensed under reduced pressure. The residue was flash chromatographed (CHCl₃:MeOH:AcOH, 60:0.5:0.5 to 30:0.5:0.5) to yield 0.578 g (80%) of intermediate coupling product (MS (ESI) m/z 301.9 [M+H]⁺). To 0.400 g (1.4 mmol) of this and NIS (0.945 g, 4.2 mmol) in acetonitrile (15 mL) was added TFA (540 μί, 0.800 g, 7 mmol) and the mixture Was stirred at room temperature overnight. The solvent was removed under reduced pressure and the residue was purified by flash chromatography

(CHCl₃:MeOH:AcOH, 60:0.5:0.5 to 30:0.5:0.5) to give S9-3 (0.436 g, 73%). 1H NMR (DMSO-de, 500 MHz) δ 8.37 (s, 1H), 8.03 (br s, 2H), 7.47 (s, 1H), 7.10 (s, 1H), 4.25-4.27 (m, 4H); MS (ESI) m/z 427.9 [M+H]⁺.

9- (2-Bromoethyl)-8-(7-iodo-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)-9H-purin-6-amine (S9-4). A mixture of S9-3 (0.213 g, 0.5 mmol), 1,2-dibromoethane (0.500 g, 2.5 mmol) and Cs₂C0₃ (0.184 g, 0.75 mmol) in anhydrous DMF (6 mL) was stirred at room temperature for 3h. Solids were filtered and the filtrate was condensed under reduced pressure to give a residue that was purified by preparative thin layer chromatography (CHCl₃:MeOH-NH₃ (7N), 20:1) to give S9-4 (0.107 g, 40%). 1H NMR (CDC1₃, 500 MHz) δ 8.29 (s, 1H), 7.36 (s, 1H), 7.00 (s, 1H), 6.23 (br s, 2H), 4.62 (t, J= 7.0 Hz, 2H), 4.16-4.24 (m, 4H), 3.69 (t, J= 7.0 Hz, 2H); MS (ESI) m/z 533.9/535.9 [M+H]⁺.

9-(3-Bromopropyl)-8-(7-iodo-2,3-dihydrobenzo[b|[l,4]dioxin-6-ylthio)-9H-purin-6- amine (S9-5). A mixture of S9-3 (0.213 g, 0.5 mmol), 1,3-dibromopropane (0.512 g, 2.5 mmol) and Cs₂C0₃ (0.184 g, 0.75 mmol) in anhydrous DMF (6 mL) was stirred at room temperature for 3h. Solids were filtered and the filtrate was condensed under reduced pressure to give a residue that was purified by preparative thin layer chromatography

(CHCl₃:MeOH-NH₃ (7N), 20:1) to give S9-5 (0.104 g, 38 %.). 1H NMR (CDC1₃, 500 MHz) δ 8.26 (s, 1H), 7.32 (s, 1H), 6.94 (s, 1H), 5.6 (br s, 2H), 4.27 (t, J= 7.0 Hz, 2H), 4.10-4.17 (m, 4H), 3.32 (t, J = 7.0 Hz, 2H), 2.26 (m, 2H); MS (ESI) m/z 547.9/549.8 [M+H]⁺. 8-(7-Iodo-2,3-dihydrobenzo[b|[l,4]dioxin-6-ylthio)-9-(2-(isobutylamino)ethyl)-5,9- dihydro^-purin-e-amine (PU-HT165). S9-4 (0.052 g, 0.097 mmol) and isobutylamine (0.354 g, 4.9 mmol) in DMF (1 ml) was stirred overnight at rt. Solvent was removed under reduced pressure and the resulting residue was purified by chromatography (CH₂Cl₂:MeOH) to give 0.040 g, 78%) of PU-HT165 as a yellow solid. 1H NMR (500 MHz, CDC1₃) δ 8.24 (s, lH), 7.37 (s, 1H), 6.94 (s, 1H), 6.24 (br s, 2H), 4.44 (br s, 2H), 4.22-4.24 (m, 4H), 3.08 (m, 2H), 2.52 (d, J= 5.7 Hz, 2H), 1.92 (m, 1H), 0.92 (d, J= 6.0 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 155.4, 152.8, 151.4, 147.7, 145.5, 145.2, 128.9, 127.5, 122.0, 120.5, 91.6, 64.9, 64.7, 57.3, 49.0, 43.9, 28.0, 21.0; MS (ESI) m/z 527.1 [M+H]⁺.

8-(7-Iodo-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)-9-(2-(neopentylamino)ethyl)-5,9- dihydro-4 -purin-6-amiiie (PU-HT175). S9-4 (0.052, 0.097 mmol) and neopentylamine (0.426 g, 4.9 mmol) in DMF (1 mL) was stirred overnight at rt. Solvent was removed under reduced pressure and the resulting residue was purified by chromatography (CH₂Cl₂:MeOH) to give 0.038 g (73%) of PU-HT175 as a yellow solid. 1H NMR (500 MHz, CDC1₃) δ 8.31 (s, 1H), 7.37 (s, 1H), 6.94 (s, 1H), 5.79 (br s, 2H), 4.33 (t, J= 6.5 Hz, 2H), 4.20-4.24 (m, 4H), 2.99 (t, J= 6.5 Hz, 2H), 2.34 (s, 2H), 0.84 (s, 9H); ¹³C NMR (125 MHz, CDC1₃) 6 154.5, 152.9, 151.7, 147.0, 144.7, 144.6, 128.2, 127.8, 121.2, 120.2, 90.7, 64.3, 64.2, 62.0, 49.8, 44.0, 31.6, 27.7; MS m/z 541.1 [M+H]⁺.

8-(7-Iodo-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine (PU-RK11). S9-5 (0.045 g, 0.082 mmol) and isopropylamine (0.242 g, 4.1 mmol) in DMF (1 mL) was stirred overnight at rt. Solvent was removed under reduced pressure and the resulting residue was purified by chromatography (CH₂Cl₂:MeOH) to give 0.038 g (88%) of PU-RK11. 1H NMR (500 MHz, CDC1₃) δ 8.30 (s, lH), 7.38 (s, 1H), 6.95 (s, 1H), 5.65 (br s, 2H), 4.32 (t, J= 6.9 Hz, 2H), 4.22-4.24 (m, 4H), 2.80 (septet, J= 6.7 Hz, 1H), 2.61 (t, J= 6.7 Hz, 2H), 2.07 (m, 2H), 1.11 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) 6 154.4, 152.8, 151.7, 146.7, 144.8, 144.6, 128.3, 127.8, 121.3, 120.1, 91.0, 64.3, 64.2, 49.0, 43.6, 41.6, 29.8, 22.5; MS (ESI) m/z 527.1 [M+H]⁺. 9-(3-(lH-imidazol-l-yl)propyl)-8-(7-iodo-2,3-dihydrobenzo [b] [1,4] dioxin-6-ylthio)-9H- purin-6-amine (PU-RK12). S9-5 (0.045 g, 0.082 mmol) and imidazole (0.056 g, 0.82 mmol) in DMF (1 mL) was stirred overnight at rt. Solvent was removed under reduced pressure and the resulting residue was purified by chromatography (CH₂Cl₂:MeOH) to give 0.029 g (67%) of PU-RK12. 1H NMR (500 MHz, CDC1₃) δ 8.34 (s, IH), 7.62 (s, IH), 7.39 (s, IH), 7.09 (s, IH), 6.98 (s, IH), 6.94 (s, IH), 5.70 (br s, 2H), 4.19-4.28 (m, 6H), 4.00 (t, J= 7.5 Hz, 2H), 2.25 (m, 2H); ¹³C NMR (125 MHz, CDC1₃) δ 154.5, 153.2, 151.8, 146.3, 145.0, 144.7, 137.1, 129.6, 128.3, 126.9, 121.3, 120.1, 118.7, 90.9, 64.3, 64.2, 44.3, 41.0, 31.8; MS (ESI) m/z 536.1 [M+H]⁺.

S10-3

S10-4

DZ3-73 R= isobutyl

DZ4-84 R= f-butyl

Reagents and conditions: (a) sulfur, morpholine, 140°C, 14 h; (b) 10% KOH (aq.), reflux, 12 h; (c)

2,4,5,6-tetraaminopyrimidine, triphenyl phosphite, pyridine, microwave irradiation at 220°C, 75 min.:

(d) HF/pyridine, NaN0₂, 0 °C to rt, 1 h; (e) NIS, TFA, CH₃CN, rt overnight; (f) Cs₂C0₃, 1,2- dibromoethane, DMF, rt, 3.5 h; (g) isobutylamine or i-butylamine, DMF, overnight, rt.

Scheme 10. Synthesis of DZ3-73 and DZ4-84.

2-(2,3-dihydrobenzo[b] [l,4]dioxin-6-yl)acetic acid (S10-2). A mixture of 1 ,4- benzodioxan-6-yl methyl ketone (SI 0-1; 5.5 g, 30.9 mmol), sulfur (1.98 g, 61.8 mmol) and morpholine (6.73 g, 6.76 mL, 77.3 mmol) was refluxed at 140°C for 14 h. After cooling to rt, the reaction mixture was diluted with 150 mL of CH₂C1₂, transferred to a seperatory funnel and washed with 25 mL of ice-cold brine. The aqueous layer was further extracted with CH₂C1₂ (2 x 75 mL). The organic layers were combined, dried with Na₂S0₄, and filtered. Activated charcoal was added to the filtrate and after several minutes was filtered and concentrated to give 12.7 g of a brown oil. A mixture of this in 75 mL of 10% KOH (ag.) was refluxed for 12h. After cooling the reaction mixture was transferred to a seperatory funnel and washed with ether (30 mL), The aqueous layer was acidified with 6N HC1 (~25 mL) to pH 2 and extracted with CH₂C1₂ (4 x 100 mL). The organic layers were combined, washed with distilled water (100 ml), dried with Na₂S0₄ and filtered. This was treated with charcoal, filtered, and solvent removed under reduced pressure and the resulting residue was purified by chromatography (hexane:EtOAc, 90:10 to 70:30) to give 3.90 g (65%) of S10-2. 1H NMR (500 MHz, CDC1₃) δ 6.80-6.82 (m, 2H), 6.74 (dd, J= 2.0, 8.2 Hz, 1H), 4.24 (s, 4H), 3.53 (s, 2H); MS (ESI) m/z 195.1 [M+H]⁺.

8-((2,3-dihydrobenzo[b] [l,4]dioxin-6-yl)methyl)-9H-purine-2,6-diamine (S10-3). A mixture of S10-2 (1.00 g, 5.15 mrnol), 2,4,5, 6-tetraaminopyrimidine (0.868 g, 6.19 mmol), triphenyl phosphite (1.92 g, 1.63 mL, 6.19 mmol) in 15 mL of pyridine was sonicated for several minutes. It was then subjected to microwave irradiation at 220°C for 75 minutes. The mixture was concentrated and the resulting residue was purified by chromatography

(CH₂Cl₂:MeOH:MeOH-NH₃ (7N), 60:0.5:0.5 to 20:0.5:0.5) to give 1.12 g (73%) of S10-3. 1H NMR (500 MHz, CDCl₃/MeOH- 4) δ 6.75-6.84 (m, 3H), 4.24 (s, 4H), 3.98 (s, 2H); MS (ESI) m/z 299.3 [M+H]⁺.

8-((2,3-dihydrobenzo[b] [l,4]dioxin-6-yl)methyl)-2-fluoro-9H-purin-6-amine (S10-4). To a solution of S10-3 (1.00 g, 3.35 mmol) in HF/pyridine (2.4 mL) at 0 °C, NaN0₂ (0.3 g, 4.36 mmol) was slowly added. The reaction was brought to room temperature and further stirred for 1 h. Following dilution with CH₂C1₂ (20 mL), the excess HF was quenched by stirring for 1 h with CaC0₃ (1.19 g). The mixture was dried under reduced pressure and subsequently purified by chromatography (CH₂Cl₂:MeOH:AcOH, 90:1 :0.5) to give 1.15 g (96%) of S10-4. 1H NMR (500 MHz, CDCl₃/MeOH-^) δ 6.75-6.84 (m, 3H), 4.24 (s, 4H), 4.04 (s, 2H); MS (ESI) m/z 302.3 [M+H]⁺. 2-fluoro-8-((7-iodo-2,3-dihydrobenzo[b|[l,4]dioxin-6-yl)methyl)-9H-purin-6-amine (SlO-5). S10-4 (0.310 g, 1.03 mmol), NIS (0.301 g, 1.34 mmol), CH₃CN (20 mL), TFA (2.34 g, 1.56 mL, 20.5 mmol) was stirred at rt overnight. The mixture was dried under reduced pressure and the residue chromatographed (CH₂Cl₂:MeOH:AcOH, 120:1 :0.5 to 90:1 :0.5) to give 0.340 g (77%) of a mixture of SlO-5 (m/z 428.2 [M+H]⁺) along wim diiodinated compound (m/z 554.1 [M+H]⁺). LC-MS shows ratio of SlO-5 to diiodinated compound to be 83:17. This mixture was not separated but used further in the following step. 1H NMR (500 MHz, CDCl₃/MeOH- O δ 7.37 (s, 1H), 6.82 (s, 1H), 4.25 (s, 4H), 4.18 (s, 2H); MS (ESI) m/z 428.2 [M+H]⁺.

9-(2-bromoethyl)-2-nuoro-8-((7-iodo-2,3-dihydrobenzo[b|[l,4]dioxin-6-yl)methyl)-9H- purin-6-amine (S10-6). SlO-5 (0.340 g, 0.796 mmol), Cs₂C0₃ (0.337 g, 1.035 mmol), 1,2- dibromoethane (0.747 g, 0.343 mL, 3.99 mmol) in DMF (10 mL) was stirred at rt for 3.5 h. The mixture was dried under reduced pressure and the residue chromatographed

(CH₂Cl₂:MeOH:AcOH, 200:1 :0.5 to 120:1 :0.5) to give 0.360 g (85%) of a mixture of S10-6 (m/z 534.0/536.2 [M+H]⁺) along with diiodinated compound (m/z 659.5/661.9 [M+H]⁺). LC- MS shows ratio of titled compound to diiodinated compound to be 80:20. This mixture was not separated but used further in the following step.

2-nuoro-8-((7-iodo-2,3-dihydrobenzo[b|[l,4]dioxin-6-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine [DZ3-73]. S10-6 (0.360 g, 0.674 mmol) and isobutylamine (2.46 g, 3.38 ml) in DMF (8 mL) was stirred overnight at rt. Solvent was removed under reduced pressure and the resulting residue was chromatographed

(CH₂Cl₂:MeOH:MeOH-NH₃ (7N), 120:0.5:0.5 to 60:0.5:0.5) to give 0.220 g of a mixture of DZ3-73 along with the diiodinated compound. This mixture was separated by reverse phase HPLC ((a) H₂0 + 0.1% TFA and (b) CH₃CN + 0.1% TFA, 10 to 75% b over 22 minutes at 16 mL/min) to give 0.196 g (58%) of DZ3-73. 1H NMR (500 MHz, CDC1₃) δ 7.35 (s, 1H), 6.57 (s, 1H), 6.37 (br s, 2H), 4.24 (s, 2H), 4.20 (s, 4H), 4.08 (t, J= 6.4 Hz, 2H), 2.91 (t, J= 6.4 Hz, 2H), 2.37 (d, J= 6.3 Hz, 2H), 1.64 (m, 1H), 0.85 (d, J= 6.2 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) 5 158.8 (d, J= 208.1 Hz), 156.4 (d, J= 19.5 Hz), 152.8 (d, J= 18.8 Hz), 151.2, 144.2, 143.6, 131.5, 127.6, 117.9, 116.7, 88.3, 64.5, 57.8, 48.8, 43.5, 38.6, 28.4, 20.5; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂oH₂₅FIN₆0₂, 527.1068; found 527.1066; HPLC: method A R_t = 6.91, method B R_t = 8.48.

9-(2-(tert-butylamino)ethyl)-2-fluoro-8-((7-iodo-2,3-dihydrobenzo[b|[l,4]dioxi yl)methyl)-9H-purin-6-amine [DZ4-84]. S10-6 (8 mg, 0.0149 mmol) and tert-butylamine (109 mg, 157 μΐ) in DMF (0.5 mL) was stirred overnight at rt. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC

(hexane:CH₂Cl₂:EtOAc:MeOH-NH₃ (7N), 7:2:1 :0.5) to give 6 mg (77%) of DZ4-84. 1H NMR (500 MHz, CDC1₃) δ 7.36 (s, IH), 6.55 (s, IH), 5.86 (br s, 2H), 4.29 (s, 2H), 4.17-4.23 (m, 4H), 4.04 (t, J= 6.4 Hz, 2H), 2.85 (t, J= 6.4 Hz, 2H), 0.99 (s, 9H); MS (ESI) m/z 527 ^'.1 [M+H]⁺.

S11-10 S11-11

Reagents and conditions: (a) NaBH₄, AcOH, THF, rt; (b) dibromoethane, acetone, H₂0, K₂C0₃, reflux; (c) BS, DMF, 80°C; (d) KN0₃, H₂S0₄; (e) Nal, Cul, Ν,Ν'-dimethylethylenediamine, dioxane, 110°C; (f) Fe, H4CI, isopropanol, reflux; (g) 8-mercaptoadenine, neocuproine, Cul, NaOtBu, DMF, 115°C; (h) Na 0₂, KI, AcOH, 0°C; (i) Cs₂C0₃, 1,2-dibromoethane or 1,3-dibromopropane, DMF, rt; (j) H₂R, DMF, rt. Scheme 11. Synthesis of Morpholine-type compounds S 11 - 11.

Reagents and conditions: (a) 1,2-dibromoethane, K₂C0₃, DMF, 125°C; (b) Mel, DMF, 0°C then rt; (c) Pd/C, H₂, MeOH, rt; (d) NaN0₂, AcOH, KI, 0°C; (e) 8-mercaptoadenine, neocuproine, Cul, NaOtBu, DMF, 115°C; (f) NIS, CH₃CN, rt; (g) Cs₂C0₃, 1,3- dibromopropane or 1,2-dibromoethane, DMF, rt; (h) H₂R, DMF, rt.

Scheme 12. Synthesis of Morpholine-type compounds SI 2-9.

6-Nitro-3,4-dihydro-2H-benzo[6] [l,4]oxazine [S12-2]. To a solution of 2-amino-4- nitrophenol (S12-1; 1.5 g, 9.7 mmol) in 50 mL of DMF was added K₂C0₃ (4.04 g, 29.2 mmol) and 1,2-dibromoethane (1 mL, 1 1.7 mmol). The resulting mixture was stirred at 125 °C under argon overnight. The resulting mixture was concentrated under vacuum and purified by flash chromatography to give S12-2 (1.2 g, 68%) as a yellow solid. 1H NMR (CDC1₃, 500 MHz) δ 7.55-7.58 (dd, J= 2.7, 8.9 Hz, 1H), 7.48 (d, J= 2.6 Hz, 1H), 6.81 (d, J= 8.9 Hz, 1H), 4.34 (m, 2H), 4.12 (br s, 1H), 3.47 (m, 2h); ¹³C NMR (CDC1₃, 125 MHz) δ 149.4, 141.8,

133.8, 115.0, 114.8, 110.2, 65.6, 40.0.

4-Methyl-6-nitro-3,4-dihydro-2H-benzo[^]l,4]oxazine [S12-3]. To a solution of S12-2 (0.66 g, 3.7 mmol) in 30 mL of DMF was added NaH (106 mg, 4.4 mmol) and stirred at 0 °C for 30 min. To the resulting mixture was added Mel (229 μϋ, 3.7 mmol) and kept stirring at rt for 2 h. The reaction mixture was concentrated in vacuum and purified by flash

chromatography to give compound S12-3 (564 mg, 79%) as yellow solid. 1H NMR (CDC1₃, 500 MHz) 6 7.56 (d, J= 8.8 Hz, 1H), 7.45 (s, 1H), 6.76 (d, J= 8.8 Hz, 1H), 4.36 (m, 2H), 3.32 (m, 2H), 2.95 (s, 3H); ¹³C NMR (CDC1₃, 125 MHz) 6: 149.7, 142.2, 136.5, 115.4, 114.5,

106.9, 65.3, 47.9, 38.6; MS (ESI) m/z 194.8 (M+H)⁺.

4- Methyl-3,4-dihydro-2H-benzo[A][l,4]oxazin-6-amine [S12-4]. To a solution of S12-3 (560 mg, 2.9 mmol) in 20 mL of methanol was added Pd/C powder (10%, 96 mg). The resulting suspension was stirred at rt under hydrogen overnight. The reaction mixture was filtered, concentrated in vacuum and purified by flash chromatography to give S12-4 (420 mg, 89%) as a yellow solid. 1H NMR (CDC1₃, 500 MHz) δ 6.56 (d, J= 8.3 Hz, 1H), 6.04 (d, J= 2.5 Hz, 1H), 5.98 (dd, J= 2.5, 8.3 Hz, 1H), 4.19 (t, J= 4.4 Hz, 2H), 3.21 (t, J= 4.5 Hz, 2H), 2.82 (s, 3H); ¹³C NMR (CDC1₃, 125 MHz) δ 140.8, 137.4, 137.0, 116.2, 104.8, 100.4, 64.6, 49.5, 38.7.

6-Iodo-4-methyl-3,4-dihydro-2H-benzo[^|[l,4]oxazine [S12-5]. To solution of S12-4 (2.1 g, 12.8 mmol) in 50 mL of acetic acid cooled in ice bath was added NaN0₂ (1.77g, 26.9 mmol) slowly in portions. The resulting mixture was stirred at 0 °C for 10 min and was added KI (4.24 g, 38.4 mmol) in portions. The reaction mixture was stirred at 0 °C for 30 min, allowed to warm up to rt and stirred for 2 h. The resulting mixture was quenched with 100 mL of water, extracted with ethyl acetate (3 x 150 mL). The organic layer was combined, treated with Na₂S₂0₃, washed with brine, dried over MgS0₄ and purified by flash

chromatography to give S12-5 (1.86 g, 53%) as a yellow solid. 1H NMR (CDC1₃, 500 MHz) δ 6.64 (d, J= 8.3 Hz, 1H), 6.56 (s, 1H), 6.48 (d, J= 8.4 Hz, 1H), 4.25 (m, 2H), 3.24 (m, 2H), 2.85 (s, 3H); ¹³C NMR (CDC1₃, 125 MHz) 6 138.1, 126.6, 120.5, 117.6, 111.9, 83.7, 64.8, 48.7, 36.5.

Reagents or conditions: (a) (C₆H₅0)₂P(0)N₃, t-BuOH, Et₃N, toluene, reflux; (b) t-BuLi, THF, -20°C, then ICH₂CH₂I, -78°C to rt; (c) TFA, CH₂C1₂, rt; (d) 8-mercaptoadenine, neocuproine, Cul, NaOtBu, DMF, 115°C; (e) KI, NaN0₂, HCl, H₂0, < 5°C; (f) 1,3-dibromopropane,

Cs₂C0₃, DMF, rt; (g) isopropylamine, DMF, rt.

Scheme 13. Synthesis of DZ4-52-N9. tert-Butyl naphthalen-2-ylcarbamate (S13-2).2-Naphthoic acid (S13-1; 2.5 g, 14.3 mmol) in tert-BuOH (85 mL) and toluene (85 mL) was treated with Et₃N (2.3 mL, 16.4 mmol), 3 A molecular sieves (16.7 g) and diphenyl phosphorylazide (3.5 mL, 16.4 mmol). The reaction mixture was refluxed for 24 h. After cooling to rt, solid was filtered off through Celite and the solvent was removed under reduced pressure. The residue was dissolved in EtOAc (75 mL) and washed with IN aqueous HCl (2 x 50 mL), saturated aqueous NaHC0₃ (2 x 50 mL), dried over sodium sulfate and concentrated under reduced pressure. Chromatography (10% EtOAc in hexanes) afforded 2.5 g (71%) of S13-2. 1H NMR (500 MHz, CDC1₃) δ 7.99 (s, 1H), 7.72-7.78 (m, 3H), 7.44 (t, J= 7.8 Hz, 1H), 7.31-7.38 (m, 2H), 6.61 (br s, 1H), 1.55 (s, 9H); MS (ESI) m/z 244.02 [M+H]⁺.

2-Amino-3-iodonaphthalene (S13-3). To a solution of S13-2 (1.0 g, 4.11 mmol) in 20 mL dry THF under argon at -20°C was added tert-butyl lithium (1.5 M solution in pentane, 6.9 mL, 10.27 mmol) dropwise and was stirred for 2 h at -20 °C. After cooling to -78°C, a solution of diiodoethane (2.9 g, 10.27 mmol) in 10 mL dry THF was added dropwise and then allowed to warm to rt for 3 h. A saturated aqueous NH₄C1 solution was added, and the solution was extracted with diethyl ether. The organic layer was washed with 10% sodium thiosulfate solution and dried over MgS0₄. The solvents were evaporated under reduced pressure and the residue was purified by chromatography (3% EtOAc in hexanes) to afford 1.1 g of a 79/21 mixture (NMR) of regioisomeric 3-iodo and 1-iodo Boc-protected 2- aminonaphthalene, respectively. This mixture (1.1 g) was dissolved in dichloromethane (12.5 mL), and trifluoroacetic acid (12.5 mL) was added dropwise at rt. After stirring for 1 h at rt, the solution was neutralized with a concentrated NaOH solution. The organic layer was separated and the aqueous layer was extracted with dichloromethane. The organic layers were combined, dried over MgS0₄, concentrated under reduced pressure and the resulting residue was purified by chromatography (0.5% EtOAc in hexanes) to afford 0.50 g (45%) of S13-3. 1H NMR (500 MHz, CDC1₃) δ 8.25 (s, 1H), 7.59 (d, J= 8.3 Hz, 1H), 7.56 (d, J= 8.3 Hz, 1H), 7.37 (dt, J= 1.0, 7.5 Hz, 1H), 7.22 (dt, J= 0.8, 7.5 Hz, 1H), 7.09 (s, 1H), 4.23 (br s, 2H); ¹³C NMR (125 MHz, CDC1₃) δ 144.3, 139.5, 135.3, 129.9, 127.5, 127.2, 126.3, 123.7, 109.0, 88.7; MS (ESI) m/z 269.96.

8-(3-aminonaphthalen-2-ylthio)-9H-purin-6-amine (S13-4). A mixture of 8- mercaptoadenine (20.7 mg, 0.124 mmol), neocuproine hydrate (3.9 mg, 0.0185 mmol), Cul (3.5 mg, 0.0185 mmol), sodium tert-butoxide (23.7 mg, 0.24 mmol), S13-3 (100 mg, 0.37 . mmol) and DMF (2 mL) were heated at 115°C for 20 h. The solvent was removed under reduced pressure and the residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 10:1) to give 14 mg (37%) of S13-4 as a solid. 1H NMR (500 MHz, CDC^/MeOH-cU) δ 8.18 (s, 1H), 8.12 (s, 1H), 7.71 (d, J= 8.3 Hz, 1H), 7.62 (d, J= 8.1 Hz, 1H), 7.40-7.46 (m, 1H), 7.24-7.30 (m, 1H), 7.20 (s, 1H); MS (ESI) m/z 308.95 [M+H]⁺. 8- (3-iodonaphthalen-2-ylthio)-9H-purin-6-amine (S13-5). To a suspension of S13-4 (14 mg, 0.0454 mmol) in water (150 μί) at 5°C was added 6 M HCl (140 μΓ) over 5 min. Then a solution of NaN0₂ (6.3 mg, 0.0908 mmol) in water (70 μΐ) was added dropwise over 30 min. at below 5°C. The mixture was stirred for an additional 10 min., then urea (1.9 mg, 0.0317 mmol) was added slowly. After 10 minutes, a solution of KI (22.6 mg, 0.136 mmol) in water (70 μί) was added dropwise over 5 min. and the mixture was stirred overnight. The solvent was removed under reduced pressure and the residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 8:1) to give 8 mg (42%) of S13-5 as a solid. 1H NMR (500 MHz, CDCl₃/MeOH-d4) δ 8.51 (s, IH), 8.17 (s, 2H), 7.75-7.80 (m, 2H), 7.52-7.60 (m, 2H); MS (ESI) m/z 420.01 [M+H]⁺.

9- (3-bromopropyl)-8-(3-iodonaphthalen-2-ylthio)-9H-purin-6-amine (S13-6). S13-5 (8 mg, 0.019 mmol), Cs₂C0₃ (7.4 mg, 0.0228 mmol), 1,3-dibromopropane (19.2 mg, 9.7 μί, 0.095 mmol) in DMF (0.2 mL) was stirred for 30 min. Then additional Cs₂C0₃ (7.4 mg, 0.0228 mmol) and 1,3-dibromopropane (19.2 mg, 9.7 μί, 0.095 mmol) was added and the mixture stirred for 30 min. The mixture was dried under reduced pressure and the residue purified by preparatory TLC (CH₂Cl₂:MeOH: AcOH, 15:1 :0.5) to give 4.6 mg (45%) of S13- 6. 1H NMR (500 MHz, CDCl₃/MeOH-d4) δ 8.51 (s, IH), 8.27 (s, IH), 8.05 (s, IH), 7.74-7.80 (m, 2H), 7.53-7.60 (m, 2H), 4.42 (t, J= 7.1 Hz, 2H), 3.45 (t, J= 6.6 Hz, 2H), 2.45 (m, 2H); MS (ESI) m/z 539.84/541.89 [M+H]⁺.

8-(3-iodonaphthalen-2-ylthio)-9-(3-(isopropylamino)propyl)-9H-purin-6-amine [DZ4- 52-N9]. S13-6 (4.6 mg, 0.0085 mmol) and isopropylamine (100 μί) in DMF (100 iL) was stirred overnight at rt. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 10:1) to give 4.0 mg (91%) of DZ4-52-N9. 1H NMR (500 MHz, CDC1₃) δ 8.44 (s, IH), 8.34 (s, IH), 7.77 (s, IH), 7.70-7.74 (m, IH), 7.64-7.68 (m, IH), 7.45-7.54 (m, 2H), 4.36 (t, J= 6.9 Hz, 2H), 2.74 (septet, J= 6.1 Hz, IH), 2.58 (t, J= 6.8 Hz, 2H), 2.06 (m, 2H), 1.05 (d, J= 6.3 Hz, 6H); MS (ESI) m/z 518.82 [M+H]⁺.

S14-1 S14-2 S14-3

S14-4 S14-5 S14-6

S14-7 S14-8

Reagents and conditions: (a)Ac₂0, dioxane, 0°C to rt; (b) KN0₃, H₂S0₄, 0°C to rt; (c) NaN0₂, KI, AcOH, 0°C to rt; (d) Fe, NH₄C1, isopropanol, reflux; (e) 8-mercaptoadenine, Cul, nBu₄NBr, NaOt- Bu, mv; (f) NaN0₂, KI, AcOH, 0°C; (g) 1 ,3-dibromopropane or 1 ,2-dibromoethane, Cs₂C0₃, DMF, rt; (h) amine, DMF, rt.

Scheme 14.

S15-1 S15-2 S15-3

- 29 n= 2, R= isopropyl PU-WS27

Reagents and conditions: (a)Ac₂0, dioxane, 0°C to rt; (b) KN0₃, H₂S0₄, 0°C to rt; (c) Na 0₂, KI, AcOH, 0°C to rt; (d) Fe, H4CI, isopropanol, reflux; (e) 8-mercaptoadenine, Cul, nBu₄NBr, NaOt- Bu, mv; (f) Na 0₂, KI, AcOH, 0°C; (g) 1,3-dibromopropane or 1,2-dibromoethane, Cs₂C0₃, DMF, rt; (h) isopropylamine or isobutylamine or neopentylamine, DMF, rt; (i) Cul, PdCl₂(PPh₃)₂,

trimethylsilanylacetylene, Et₃N, DMF, 90°C.

Scheme 15. Synthesis of PU-WS25, PU-WS26, PU-WS29 and PU-WS27.

5-amino-6-nitro-indane (S15-2). A solution of 5-aminoindane (S15-1; 10 g, 75 mmol) in 100 mL of dioxane cooled in ice bath was added acetic anhydride (15 mL) dropwise and kept stirring at room temperature for 2 days. The resulting mixture was condensed and dried under vacuum. The residue was dissolved in 100 mL of concentrated H₂S0₄, cooled in ice bath. KN0₃ in 15 mL of concentrated H S0₄ was added dropwise. The resulting solution was stirred at 0 °C for 2 h and then at rt for 2 h. The reaction mixture was poured into 150 g of ice and the resulting yellow precipitate was filtered and washed with cold water to give S15-2 (7.1 g, 43%). 1H NMR (500 MHz, CDC1₃) δ 7.94 (s, IH), 6.65 (s, IH), 6.02 (br, 2H), 2.83 (m, 4H), 2.06 (m, 2H); ¹³CNMR (125 MHz, CDC1₃) δ 154.4, 144.2, 134.1, 131.2, 120.8, 113.5, 33.1, 31.4, 25.7. 5-iodo-6-nitro-indane (S15-3). To a solution of S15-2 (0.14 g, 0.78 mmol) in acetic acid cooled in ice bath was added NaN0₂ (65 mg, 0.94 mmol). The reaction mixture was stirred for 2 minutes. KI (0.39g, 2.45 mmol) was added and the mixture was stirred at rt for 20 minutes. The resulting suspension was quenched with water (15 mL) and extracted with ethyl acetate (2 x 20 mL). The organic layer was washed with saturated aqueous Na₂S₂0₃ solution, brine and dried over MgS04 and evaporated to dryness to give a residue that was purified by flash chromatography (ethyl acetate/hexane, gradient 0 to 50%) to give S15-3 (0.12 g, 65%) as a yellow solid. ¹HNMR (500 MHz, CDCI₃) δ 7.83 (s, 1H), 7.71 (s, 1H), 2.95 (m, 4H), 2.11 (m, 2H).

5-amino-6-iodo-indane (S15-4). To a solution of S15-3 (1.65 g, 5.7 mmol) in isopropanol (100 mL) and saturated aqueous NH₄C1 solution (20 mL) was added iron powder (1.1 g). The resulting suspension was refluxed for lh. The reaction mixture was filtered and the filtrate was condensed and purified by flash chromatography (ethyl acetate/hexane, gradient 0 to 50%) to give S15-4 (1.36 g, 92%) as a pale yellow solid. 1H NMR (500 MHz, CDC1₃) δ 7.44 (s, lH), 6.59 (s, 1H), 3.88 (s, 2H), 2.74 (m, 4H), 1.98 (m, 2H); ¹³CNMR (125 MHz, CDC1₃) δ 146.2, 144.9, 136.5, 134.1, 111.0, 32.8, 31.8, 26.1; MS (ESI): m/z 259.99 [M+H]⁺.

8-((6-anrino-2,3-dihydro-l^-inden-5-yl)thio)-9-^-purin-6-amine (S15-5). The mixture of 8-mercaptoadenine (64 mg, 0.38 mmol), S15-4 (100 mg, 0.38 mmol), Cul. (14.7 mg, 0.07 mmol), sodium t-butoxide (111 mg, 1.15 mmol) and tetrabutylammonium bromide (24.9 mg, 0.07 mmol) in anhydrous DMF (4 mL) was vortexed and heated at 190 °C under microwave for lh. The resulting mixture was condensed and purified by flash chromatography

(methylene chloride/methanol, gradient 0 to 10%) to give S15-5 (54 mg, 47%) as a while solid. ¹HNMR (500 MHz, MeOH-^/CDCl₃) δ 8.11 (s, 1H), 7.36 (s, 1H), 6.81 (s, lH), 2.85 (m, 4H), 2.06 (m, 2H); MS (ESI): m/z 299.02 [M+H]⁺.

8-((6-iodo-2,3-dihydro-lfl-inden-5-yI)thio)-9-fl-purin-6-amine (S15-6). To a solution of S15-5 (54 mg, 0.18 mmol) in acetic acid (5 mL) cooled in ice bath was added NaN0₂ (15 mg, 0.22 mmol) followed by KI (90 mg, 0.54 mmol). The reaction mixture was stirred at 0 °C for 15 min and quenched with water (10 mL). The resulting mixture was extracted with methylene chloride (2 x 20 mL). The organic layer was washed with saturated aqueous Na₂S₂0₃, brine, dried over MgS0₄ and evaporated to dryness. The residue was purified by flash chromatography (methylene chloride/methanol, gradient 0 to 10%) to give S15-6 (42 mg, 56%) as a white solid. ¹HNMR (500 MHz, CDC1₃) δ 8.12 (s, 1H), 7.84 (s, 1H), 7.39 (s, 1H), 2.91 (m, 4H), 2.11 (m, 2H); MS (ESI) m/z 410.10 [M+H]⁺.

9^2-bromoethyl)-8-((6-iodo-dihydro-lH-inden-5-yl)thio)-9H-purin-6-ainine (S15-7A).

To a solution of S15-6 (70 mg, 0.17 mmol) in DMF (3 mL) was added 1,2-dibromoethane (74 uL, 0.86 mmol) and Cs₂C0₃ (111 mg, 0.34 mmol). The resulting mixture was stirred at rt for 2 h. SI 5-7 A (36 mg, 41%) was obtained following preparatory TLC (methylene chloride/methanol, 20/1) as a white solid. ¹HNMR (500 MHz, CDC1₃) δ 8.36 (s, 1H), 7.75 (s, 1H), 7.18 (s, 1H), 4.62 (t, 2H), 3.68 (t, 2H), 2.88 (t, 2H), 2.81 (t, 2H), 2.06 (m, 2H); ¹³CNMR (125 MHz, CDC1₃, δ): 155.9, 153.9, 152.4, 149.8, 148.9, 148.1, 137.6, 132.8, 131.1, 101.7, 46.3, 33.9, 29.7, 26.8; MS (ESI): m/z 516.15, 518.16 [M, M+2]⁺.

8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine (PU-WS25). To a solution of S15-7A (31 mg, 0.06 mmol) in DMF (1.5 mL) was added neopentylamine (250 uL). The reaction mixture was stirred at rt overnight and condensed under vacuum. PU-WS25 (28 mg, 89%) was obtained following preparatory TLC (methylene chloride/methanol, 10/1) as a white solid. 1H MR (500 MHz, CDC1₃) δ 8.32 (s, 1H), 7.73 (s, 1H), 7.1 (s, 1H), 5.63 (br, 2H), 4.38 (m, 2H), 3.03 (m, 2H), 2.87 (t, J= 7.4 Hz, 2H), 2.78 (t, J= 7.4 Hz, 2H), 2.37 (s, 2H), 2.04 (m, 2H), 0.93 (s, 9H); ¹³C NMR (125 MHz, CDC1₃, δ):154.7, 152.9, 151.6, 147.1, 146.7, 146.4, 135.9, 133.5, 127.5, 120.2, 97.7, 61.8, 50.7, 49.7, 43.9, 32.5, 32.2, 31.5, 27.7, 25.5; HRMS (m/z): [M+H]⁺ calcd for C₂₁H₂₈IN₆S, 523.1141 ; found 523.1140.

8-((6-iodo-2,3-dihydro-lH-inden-5-yI)thio)-9-(2-(isobutylamino)ethyl)-9H-purin-6- amine (PU-WS26). To a solution of S15-7A (6 mg, 0.01 mmol) in DMF (1 mL) was added isobutylamine (150 uL). The reaction mixture was stirred at rt overnight and condensed under vacuum. PU-WS26 (5.9 mg, 99%) was obtained following preparatory TLC (methylene chloride/methanol, 10/1) as a white solid. ¹HNMR (500 MHz, CDC1₃) δ 8.31 (s, 1H), 7.74 (s, 1H), 7.11 (s, 1H), 5.73 (br, 2H), 4.43 (m, 2H), 3.04 (m, 2H), 2.87 (t, J- 7.4 Hz, 2H), 2.78 (t, J= 7.4 Hz, 2H), 2.49 (d, J= 6.6 Hz, 2H), 2.05 (m, 2H), 1.81 (m, 1H), 0.92 (m, 6H); HRMS (m/z): [M+H]⁺ calcd for C₂₀H₂₆IN₆S 509.0984; found 509.0990.

9-(3-bromopropyl)-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9H-purin-6-amine (S15- 7B). To a solution of S15-6 (30 mg, 0.07 mmol) in DMF (3 mL) was added 1 ,3- dibromopropane (37 μΐ,, 0.86 mmol) and Cs₂C0₃ (46 mg, 0.14 mmol). The resulting mixture was stirred at rt for 2 h. S15-7B (8 mg, 21%) was obtained following preparatory TLC (methylene chloride/methanol, 20/1) as a white solid. 1H NMR (500 MHz, CDC1₃) δ 8.27 (s, 1H), 7.75 (s, 1H), 7.12 (s, 1H), 6.55 (br s, 2H), 4.33 (m, 2H), 2.88 (m, 2H), 2.79 (t, J= 7.4 Hz, 2H), 2.29 (m, 2H), 1.97 (m, 2H); MS (ESI): m/z 530.3, 532.3[M, M+2]⁺.

8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9-(3-(isopropyIamino)propyI)-9H-purin-6- amine (PU-WS29). To a solution of S15-7B (8 mg, 0.015 mmol) in DMF (3 mL) was added isopropylamine (100 \iL), stirred at rt overnight and condensed under vacuum. PU-WS29 (5.9 mg, 99%) was obtained following preparatory TLC (methylene chloride/methanol, 10/1) as a white solid. 1H NMR (500 MHz, CDC1₃)5 8.32 (s, 1H), 7.75 (s, 1H), 7.12 (s, 1H), 5.73 (br s, 2H), 4.29 (t, 2H), 2.87 (t, J= 7.4 Hz, 2H), 2.7-2.79 (m, 3H), 2.55 (t, 2H), 2.03-2.09 (m, 4H), 1.05 (d, J= 11.2 Hz, 6H); ¹³C NMR (125 MHz, CDC1₃) δ 154.5, 152.9, 151.7, 147.2, 146.5, 135.9, 133.1, 127.6, 120.2, 97.9, 48.8, 43.7, 41.7, 32.5, 32.2, 30.0, 25.5, 22.7; HRMS (m/z): [M+H]⁺ calcd for C₂₀H₂₆IN₆S 509.0984; found 509.1003.

8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine (PU-WS27). Following the procedure to make PU-WS8, PU-WS27 was obtained from PU-WS25 as a white solid. 1H NMR (CDC1₃, 500 MHz) δ 8.32 (s, 1H), 7.41 (s, 1H), 7.13 (s, 1H), 5.67 (br s, 2H), 4.42 (m, 2H), 3.48 (s, 1H), 3.02 (m, 2H), 2.77-2.91 (m, 4H), 2.39 (s, 2H), 2.06 (m, 2H), 0.89 (s, 9H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₈N₆S, 421.2174; found 421.2164.

PU-WS18 n= 2, R= isopropyl

S16-10A n= 2

S16-10B n= 1 PU-WS17 n= 1 , R= isobutyl

PU-WS22 n= 1 , R= neopentyl

Reagents and conditions: (a)EtOH, H₂S0₄, reflux; (b) 2-bromomethylmalonate, NaH, DMF, 110°C; (c) PPA, toluene, reflux; (d) NaOH, MeOH, it, then HC1; (e) Pd/C, H₂ (2 atm.), MeOH; (f) 2,4,5,6-tetraaminopyrimidine, triphenyl phosphite, pyridine, microwave, 210°C; (g)

HF/pyridine, NaN0₂, 0°C to rt; (h) NIS, TFA, ACN; (i) 1,3-dibromopropane or 1,2- dibromoethane, Cs₂C0₃, DMF, rt; (h) isopropylamine or isobutylamine or neopentylamine, DMF, rt.

Scheme 16. Synthesis of PU-WS17, PU-WS18, PU-WS22.

Ethyl 2-(3-hydroxyphenyI)acetate (S16-2). To a solution of 2-(3-hydroxyphenyl)acetic acid (S16-1; 10 g, 65.8 mmol) in 200 mL of ethanol was added 8 mL of concentrated sulfuric acid. The resulting mixture was refluxed overnight and condensed under vacuum. The residue was dissolved in ethyl acetate and washed with water. The organic layer was combined, washed with brine, dried over MgS0₄, evaporated to dryness and purified by flash chromatography to give S16-2 as a colorless oil in quantitative yield. 1H NMR (500 MHz, CDC1₃) δ 7.35 (br, 1H), 7.12 (m, 1H), 6.69-6.78 (m, 3H), 4.12 (m, 2H), 3.53 (s, 2H), 1.21 (m, 3H).

Diethyl 2-((3-ethoxy-2-oxoethyL)phenox)methyL)malonate (S16-3). To a solution of S16-2 (11.8 g, 65.5 mmol) in 150 mL of DMF cooled in ice bath was added NaH (2.36 g, 98 mmol) and stirred at 0 °C under argon for 20 min. To the resulting mixture was added diethyl 2- bromomethylmalonate (11.8 mL, 78 mmol) dropwise. The reaction mixture was stirred at 110 °C overnight, evaporated to dryness and purified by flash chromatography to give compound S16-3 (15.2 g, 66%) as a colorless oil. 1H NMR (500 MHz, CDC1₃) δ 7.19 (t, 1H), 6.80-6.86 (m, 3H), 4.81 (m, 1H), 4.12 (m, 2H), 3.97 (m, 2H), 3.74 (m, 2H), 3.63 (m, 2H), 3.55 (s, 2H), 1.19 (m, 9H); ¹³C NMR (125 MHz, CDC1₃) δ 171.3, 158.8, 135.6, 129.5, 121.8, 115.6, 113.3, 100.5, 68.5, 62.5, 60.7, 41.3, 15.4, 14.1.

Ethyl 2-(benzofuran-6-yl) acetate (S16-4). To a solution of S16-3 (6 g, 17 mmol) in 100 mL of toluene was added 3 g of polyphosphoric acid. The resulting mixture was refluxed overnight, condensed and purified by flash chromatography to give S16-4 (1.42 g, 41%) as colorless oil. 1H NMR (500 MHz, CDC1₃) δ 7.31-7.42 (m, 3H), 6.95 (m, 1H), 6.51 (s, 1H), 3.94 (m, 2H), 3.51 (s, 2H), 1.02 (m, 3H); ¹³C NMR (125 MHz, CDC1₃) δ 171.6, 155.2, 145.1, 130.6, 126.4, 124.3, 121.1, 112.2, 106.4, 60.9, 41.5, 14.2.

2-(benzofuran-6-yL) acetic acid (S13-5). To a solution of S16-4 (3 g, 14.7 mmol) in 100 mL methanol was add 25 mL of 1 N NaOH. The resulting mixture was stirred at rt for 2 h, neutralized with concentrated HC1, and adjusted pH to 2. The reaction mixture was condensed, purified by flash chromatography to yield S16-5 as a white solid in quantitative yield. 1H NMR (500 MHz, MeOH-<¾ δ 7.73 (s, 1H), 7.53 (d, J= 7.9 Hz, 1H), 7.43 (s, 1H), 7.15 (d, J= 8.0 Hz, 1H), 6.79 (s, 1H), 3.70 (s, 2H); ¹³C NMR (125 MHz, MeOH-<¾ δ 175.7, 156.6, 146.6, 132.5, 127.7, 125.4, 121.9, 113.0, 107.4, 41.9.

2-(2,3-dihydrobenzofuran-6-yl)acetic acid (S16-6). To a solution of S16-5 (1.8 g, 10 mmol) in 20 mL of methanol was added Pd/C (10%, 120 mg) and stirred at rt under H₂ (2 atm) overnight. The reaction mixture was filtered, washed with cold methanol, evaporated to dryness and purified by flash chromatography to give S16-6 (1.6 g, 88%) as a white solid. 1H NMR (500 MHz, MeOH-<¾ δ 7.12 (d, J= 7.6 Hz, IH), 6.73 (d, J= 7.9 Hz, IH), 6.71 (s, IH), 4.55 (m, 2H), 3.56 (s, 2H), 3.16 (m, 2H); ¹³C NMR (125 MHz, MeOH-cU) δ 177.9, 160.4, 133.3, 126.2, 124.8, 121.5, 110.5, 71.5, 41.1, 29.4.

8-((2,3-dihydrobenzofuran-6-yl)methyI)-9H-purine-2,6-diamine (S16-7). The mixture of 2,4,5,6-tetraaminopyrimidine (200 mg, 1.4 mmol), S16-6 (254 mg, 1.4 mmol) and triphenyl phosphite (451 μΐ., 1.7 mmol) in 2 mL of pyridine was irradiated in the microwave for 15 min at 210 °C. After cooling, the reaction mixture was concentrated under vacuum and the residue purified by flash chromatography to give S16-7 (350 mg, 89%) as a yellow solid. ^!H NMR (500 MHz, MeOH-i¾) δ 7.16 (m, IH), 6.79 (m, IH), 6.73 (s, IH), 4.57 (m, 2H), 4.12 (s, 2H), 3.18 (m, 2H); MS: m/z 283.2 (M+H)⁺.

8-((2,3-dihydrobenzofuran-6-yI)methyI)-2-fluoro-9H-purin-6-amine (S16-8). A plastic tube charged with S16-7 (0.72 g, 2.5 mmol) was cooled in ice bath, added HF/pyridine (73%, 1.76 mL) and stirred to dissolve. To the resulting mixture was added NaN0₂ (0.23 g, 3.3 mmol) in portions and kept stirring for 5 min. The reaction mixture was allowed to warm up to rt and stirred for 3 h. CaC0₃ (0.68 g) was added to quench excess HF. The resulting suspension was stirred for 1 h, filtered, concentrated in vacuo and purified by flash chromatography to give S16-8 ( 0.45 g, 62%) as a yellow solid. 1H NMR (500 MHz, MeOH- d₄) δ: 7.16 (m, IH), 6.77 (m, IH), 6.71 (s, IH), 4.57 (m, 2H), 4.12 (s, 2H), 3.19 (m, 2H); MS: m/z 286.0 (M+H)⁺.

2- fluoro-8-((5-iodo-2,3-dihydrobenzofuran-6-yl)methyl)-9H-purin-6-amine (S16-9). To a suspension of S16-8 (0.45 g, 1.6 mmol) in 50 mL acetonitrile was added 1 mL of TFA. To the resulting solution was added NIS (1.06 g, 4.7 mmol) and the reaction mixture was stirred at rt for 3 h. It was then evaporated to dryness and purified by flash chromatography to give S16-9 (0.408 g, 63%) as a yellow solid. ¹H NMR (500 MHz, MeOH-i¾ δ 7.67 (s, IH), 6.76 (s, IH), 4.59 (m, 2H), 4.28 (s, 2H), 3.21 (m, 2H); MS: m/z 412 (M+H)⁺. 9- (3-bromopropyl)-2-fluoro-8-((5-iodo-2,3-dihydrobenzofuran-6-yl)methyl)-9H-purin- 6-amine (S16-10A). To a solution of SI 6-9 (50 mg, 0.12 mmol) in 2 mL of DMF was added 1,3-dibromopropane (150 μϋ) and Cs₂C0₃ (80 mg, 0.24 mmol). The resulting mixture was stirred at rt for 2 h, evaporated to dryness and purified by preparatory TLC to give S16-10A (23 mg, 36%) as a white solid. 1H NMR (500 MHz, MeOH-i¾ δ 7.48 (s, 1H), 6.31 (s, 1H), 4.35 (m, 2H), 4.12 (s, 2H), 3.92 (m, 2h), 3.14 (m, 2H), 3.01 (m, 2H), 2.03 (m, 2H); MS: m/z 530, 532 (M, M+2)⁺.

2-fluoro-8-((5-iodo-2,3-dihydrobenzofuran-6-yl)methyl)-9-(3-(isopropylamino)propyl)- 9H-purin-6-amine (PU-WS18). To a solution of S16-10A (1 mg, 0.03 mmol) in 1 mL of DMF was added isopropylamine (0.5 mL), stirred at rt overnight, evaporated to dryness and purified by flash chromatography to give PU-WS18 (13 mg, 90%) as a white solid. 1H NMR (500 MHz, MeOH-i¾ 6 7.67 (s, 1H), 6.72 (s, 1H), 4.63 (m, 2H), 4.26 (m, 4H), 3.22-3.29 (m, 3H), 2.93 (t, J= 7.1 Hz, 2H), 2.27 (t, J= 7.0 Hz, 2H), 1.38 (d, J= 6.5 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₀H₂₅N₆OFI, 511.1119; found 511.1103.

2-fluoro-8-((5-iodo-2,3-dihydrobenzofuran-6-yl)methyl)-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine [PU-WS17]. To a solution of S16-9 (70 mg, 0.17 mmol) in 2 mL of DMF was added 1,2-dibromoethane (150 μί) and Cs₂C0₃ (110 mg, 0.34 mmol). The resulting mixture was stirred at rt for 2 h, evaporated to dryness and purified by preparatory TLC to give bromide intermediate S16-10B. To a solution of S16-10B (10 mg, 0.19 mmol) in 1 mL of DMF was added isobutylamine (100 uL), stirred at rt overnight, evaporated to dryness and purified by flash chromatography to give PU-WS17 as a white solid. 1H NMR (MeOH- d4/CDCl₃, 500 MHz) 8: 7.67 (s, 1H), 6.62 (s, 1H), 4.59 (t, J = 8.7 Hz, 2H), 4.29 (s, 2H), 4.15 (t, J = 6.5 Hz, 2H), 3.22 (t, J = 8.7 Hz, 2H), 2.93 (t, J = 6.5 Hz, 2H), 2.45 (d, J = 6.9 Hz, 2H), 1.69 (m, 1H), 0.88 (d, J = 6.8 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₀H₂₅FIN₆O, 511.1119; found 511.1113.

2-fluoro-8-((5-iodo-2,3-dihydrobenzofuran-6-yl)methyl)-9-(2-(neopentylamino)ethyl)- 9H-purin-6-amine [PU-WS22]. To a solution of S16-9 (70 mg, 0.17 mmol) in 2 mL of DMF was added 1,2-dibromoethane (150 μί) and Cs₂C0₃ (110 mg, 0.34 mmol). The resulting mixture was stirred at rt for 2 h, evaporated to dryness and purified by preparatory TLC to give bromide intermediate S16-10B. To a solution of S16-10B (65 mg, 0.13 mmol) in 1 mL of DMF was added neopentylamine (50 μΐ,), stirred at rt overnight, evaporated to dryness and purified by flash chromatography to give compound PU-WS22 as a white solid. 1H NMR (CDC1₃, 500 MHz) δ 7.46 (s, IH), 6.53 (s, IH), 5.79 (s, 2H), 5.52 (br, 2H), 4.52 (m, 2H), 4.09 (m, 2H), 3.19 (m, 2H), 2.94-3.02 (m, 2H), 2.34(s, 2H), 0.91 (s, 9H); HRMS (ESI) m/z

[M+H]⁺ calcd. for C₂₁H₂₇FIN₆0, 525.1275; found 525.1249.

S17-7

S17-8

Reagents and conditions: (a) HN0₃, H₂S0₄; (b) Nal, Cul, Ν,Ν'-dimethylethylenediamine, dioxane, 110°C; (C) Fe, HC1; (d) 8-mercaptoadenine, neocuproine, Cul, NaOtBu, DMF, 115°C; (e) KI, NaN0₂, HC1, < 5°C ; (f) Cs₂C0₃, 1,3-dibromopropane, DMF, rt; (g) isopropylamine, DMF, rt.

Scheme 17.

Reagents and conditions: (a) Ac₂0, CH₂C1₂, rt; (b) Br₂, AcOH, 10°C; (c) Cr0₃, AcOH/H₂0, 50-55°C; (d) Nal, Cul, Ν,Ν'-dimethylethylenediamine, dioxane, 110°C; (e) 6M HCl (aq.), reflux; (f) 8- mercaptoadenine, neocuproine, Cul, NaOtBu, DMF, 115°C; (g) KI, NaN0₂, HCl, < 5°C ; (h) Cs₂C0₃, 1,3-dibromopropane, DMF, rt; (i) isopropylamine, DMF, rt.

Scheme 18.

PU-HJP18 X= H, Z= S, n= 2, R= isopropyl, X= 2-furanyl

PU-RK11 X= H, Z= S, n= 2, R= isopropyl PU-HJP19 X= H, Z= S, n= 2, R= isopropyl, X= 3-pyrazolyl PU-HT16S X= H, Z= S, n= 1 , R= isobutyl PU-HJP23 X= H, Z= S, n= 1 , R= isobutyl, X= 2-furanyl DZ3-73 X= F, Z= CH₂, n= 1, R= isobutyl TT-VI-53A X= F, Z= CH₂, n= 1 , R= isobutyl, X= 2-furanyl

TT-VI-54A X= F, Z= CH₂, n= 1 , R= isobutyl, X= 3-pyrazolyl PU-HJP20 X= H, Z= S, n= 2, R= isopropyl, X= 2-oxazolyl

Reagents or conditions: (a) boronic acid, PdCl₂(PPh₃)₂, NaHC0₃, H₂0, DMF; (b) XSn(Bu)₃, LiCl, Pd(PPh₃)₄, DMF, 90°C . Scheme 19. Cross-coupling reactions of PU-RKl l, PU-HT 165 and DZ3-73.

8-((7-(furan-2-yl)-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine [HJP18]. 2-Furanylboronic acid (8 mg, 0.0712 mmol) was added to PU-RKll (25 mg, 0.0475 mmol) and NaHC0₃ (12 mg, 0.1425 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (6.7 mg, 0.0095 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 12 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 10.2 mg (45%) of HJP18. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 8.29 (s,lH), 7.47 (s, 1H), 7.26 (d, J= 3.8 Hz, lH), 6.89 (s, 1H), 6.73 (d, J= 3.9 Hz, 1H), 6.46 (m, 1H), 4.25 (m, 4H), 4.16 (t, J= 6.2 Hz, 2H), 2.67 (m, 1H), 2.47 (t, J = 7.1 Hz, 2H), 1.86 (m, 2H), 1.01 (d, J= 6.2 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH- d4) δ 154.4, 152.8, 151.7, 151.0, 146.7, 144.2, 143.7, 142.2, 126.6, 122.1, 119.9, 119.3, 117.6, 111.5, 109.5, 64.4, 64.3, 48.6, 43.8, 41.6, 30.1, 22.8; MS (ESI) m/z 467.14 [M+H]⁺.

8-((7-(lH-pyrazol-3-yl)-2,3-dihydrobenzo[b|[l,4]dioxin-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amiiie [HJP19]. lH-Pyrazole-3-boronic acid (6.4 mg, 0.057 mmol) was added to PU-RKll (20 mg, 0.038 mmol) and NaHC0₃ (9.8 mg, 0.117 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (5.3 mg, 0.0076 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 12 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 15:1) to give 7.6 mg (43%) of HJP19. Additionally, 15.9 mg of unreacted PU-RK11 was recovered for an actual yield of 86%. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 8.18 (s, 1H), 7.53 (d, J= 2.4 Hz, 1H), 7.15 (s, 1H), 7.14 (s, 1H), 6.36 (d, J= 2.4 Hz, 1H), 4.29 (m, 4H), 4.19 (t, J= 6.6 Hz, 2H), 2.75 (septet, J= 6.1 Hz, 1H), 2.52 (t, J= 6.6 Hz, 2H), 1.93 (m, 2H), 1.06 (d, J= 6.1 Hz, 6H); MS (ESI) m/z 468.0 [M+H]⁺. 8 (7 furan-2-yl)-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9-(2-(isobutylamino)ethy^ 9H-purin-6-amine [HJP23]. 2-Furanylboronic acid (5.4 mg, 0.0486 mmol) was added to PU-HT165 (9 mg, 0.0171 mmol) and NaHC0₃ (5.7 mg, 0.0684 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (2.4 mg, 0.0034 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 12 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 1.8 mg (23%) of HJP23. 1H NMR (500 MHz, CDC1₃) δ 8.29 (s, 1H), 7.48 (d, J= 1.8 Hz, 1H), 7.26 (s, 1H), 6. 90 (s, 1H), 6.74 (d, J= 3.2 Hz, 1H), 6.47 (m, 1H), 5.63 (br s, 2H), 4.20-4.30 (m, 6H), 2.90 (t, J= 6.0 Hz, 2H), 2.38 (d, J= 6.8 Hz, 2H), 1.65 (m, 1H), 0.85 (d, J= 6.9 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₇N₆0₃S, 467.1865; found 467.1884.

2-fluoro-8-((7-(furan-2-yl)-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine [TT-VI-53A]. 2-Furanylboronic acid (8 mg, 0.0712 mmol) was added to DZ3-73 (25 mg, 0.0475 mmol) and NaHC0₃ (12 mg, 0.1425 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (6.7 mg, 0.0095 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 12 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 20.9 mg (94%) of TT-VI-53A. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 7.45 (d, J= 1.8 Hz, 1H), 7.13 (s, 1H), 6.60 (s, 1H), 6.44 (dd, J= 1.8, 3.3 Hz, 1H), 6.35 (d, J= 3.3 Hz, lH), 4.34 (s, 2H), 4.26 (s, 4H), 4.05 (t, J= 6.4 Hz, 2H), 2.85 (t, J= 6,4 Hz, 2H), 2.35 (d, J= 6.9 Hz, 2H), 1.67 (m, 1H), 0.85 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-i#) δ 158.8 (d, J= 209.1 Hz), 156.3 (d, J= 19.5 Hz), 152.8, 152.2, 143.9, 142.9, 142.2, 126.0, 124.1, 118.7, 117.7, 117.6, 116.3, 111.6, 108.2, 64.6, 57.5, 48.6, 43.1, 31.8, 28.2, 20.6; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₄H₂₈FN₆0₃, 467.2207; found 467.2203; HPLC: method A R_t = 7.05, method B R_t = 8.74. 8- ((7-(lH-pyrazol-3-yl)-2,3-dihydrobenzo [b] [1,4] dioxin-6-yl)methyl)-2-fluoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-ainine [TT-VI-54A]. lH-Pyrazole-3-boronic acid (26 mg, 0.228 mmol) was added to DZ3-73 (30 mg, 0.0570 mmol) and NaHCQ₃ (29 mg, 0.342 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.2 mL) and Pd(PPh₃)₂Cl₂ (8 mg, 0.0114 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 12 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 15:1) to give 11.3 mg (42%) of TT-VI-54A. Additionally, 15.9 mg of unreacted DZ3-73 was recovered for an actual yield of 90%. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 7.60 (d, J= 2.1 Hz, 1H), 7.02 (s, 1H), 6.82 (s, 1H), 6.33 (d, J= 2.1 Hz, 1H), 4.32 (t, J= 6.8 Hz, 2H), 4.29 (s, 2H), 4.28 (s, 4H), 2.96 (t, J= 6.8 Hz, 2H), 2.59 (d, J = 7.0 Hz, 2H), 1.92 (m, 1H), 0.96 (d, J= 6.7 Hz, 6H); ¹³C NMR (125 MHz, CDCl₃/MeOH-i¾ δ 158.2 (d, J= 210.1 Hz), 156.5 (d, J= 19.9 Hz), 152.6, 152.2, 152.1, 144.0, 143.0, 126.5, 119.1, 118.9, 115.94, 115.91, 105.4, 64.65, 64.56, 56.5, 47.7, 41.0, 31.1, 27.2, 20.3; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₈FN₈0₂, 467.2319; found 467.2323; HPLC: method A R_t = 6.39, method B R_t = 7.03.

9- (3-(isopropylamino)propyl)-8-((7-(oxazoI-2-yI)-2,3-dihydrobenzo [b] [1,4] dioxin-6- yl)thio)-9H-purin-6-amine [HJP20]. 2-(Tributyltin)oxazole (54 mg, 0.1518 mmol) was added to PU-RKll (20 mg, 0.038 mmol) and LiCl (3.2 mg, 0.076 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then Pd(PPh₃)₄ (6.7 mg, 0.0095 mmol) was added and the reaction mixture was heated under nitrogen at 90°C for 12 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 4.7 mg (27%) of HJP20. Additionally, 7 mg of unreacted PU-RKl 1 was recovered for an actual yield of 45%. 1H NMR (500 MHz, CDC1₃) δ 8.32 (s, 1H), 7.70 (s, 1H), 7.54 (s, IH), 7.26 (s, 1H), 6.59 (s, 1H), 5.79 (br s, 2H), 4.20-4.34 (m, 6H), 2.67 (m, J= 6.1 Hz, 1H), 2.50 (t, J= 6.8 Hz, 2H), 1.93 (m, J= 7.1 Hz, 2H), 0.99 (d, J= 6.4 Hz, 6H); MS (ESI) m/z 468.15 [M+H]⁺.

EC102

Reagents or conditions: (a) RB(OH)₂, PdCl₂(PPh₃)₂, NaHC0₃, H₂0, DMF; (b) Cul, PdCl₂(PPh₃)₂, trimethylsilanylacetylene, Et₃N, DMF, 90°C.

Scheme 20. Cross-coupling reactions of EC 102.

8-(2-(furan-2-yl)-5-methoxyphenylthio)-9-(2-(neopentylamino)ethyl)-9H-purin-6-amin

[TT-V-138]. 2-Furanylboronic acid (8.2 mg, 0.0732 mmol) was added to EC102 (25 mg,

0.0488 mmol) andNaHC0₃ (12.3 mg, 0.1464 mmol). DMF (1 mL) was added and the

reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.1 mL) and Pd(PPh₃)₂Cl₂ (6.8 mg, 0.00976 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20: 1) to give 20.7 mg (94%) of TT-V-138. 1H NMR (500 MHz, CDC1₃) δ 8.31 (s, l H), 7.62 (d, J= 8.7 Hz, 1H), 7.50 (d, J= 1.8 Hz, 1H), 6.86 (dd, J= 2.6, 8.7 Hz, 1H), 6.70-6.73 (m, 2H), 6.49 (dd, J= 1.8, 3.3 Hz, 1H), 5.98 (br s, 2H), 4.26 (t, J= 6.2 Hz, 2H), 3.70 (s, 3H), 2.89 (t, J= 6.2 Hz, 2H), 2.28 (s, 2H), 0.84 (s, 9H); ¹³C NMR (125 MHz, CDC1₃) δ 159.4, 154.8, 153.2, 151.6, 151.2, 145.4, 142.0, 130.9, 130.2, 124.3, 120.1, 116.5, 113.4, 111.4, 109.1, 61.8, 55.3, 49.6, 43.9, 31.5, 27.6; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₉N₆0₂S, 453.2073; found 453.2071; HPLC: method A R_t = 6.76, method B R_t - 7.29.

8-(5-methoxy-2-(thiophen-2-yl)phenylthio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine [TT-V-139]. 2-Thiopheneboronic acid (18.8 mg, 0.147 mmol) was added to EC102 (25 mg, 0.0488 mmol) and NaHC0₃ (24.6 mg, 0.293 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.25 mL) and Pd(PPh₃)₂Cl₂ (10.4 mg, 0.0148 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 5 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 16.2 mg (71%) of TT-V-139. 1H NMR (500 MHz, CDCl₃/MeOH-c¾) δ 8.19 (s, 1H), 7.45 (d, J= 8.5 Hz, 1H), 7.30-7.33 (m, 1H), 6.99-7.04 (m, 3H), 6.97 (dd, J= 2.6, 8.5 Hz, 1H), 4.24 (t, J= 6.1 Hz, 2H), 3.82 (s, 3H), 2.97 (t, J= 6.1 Hz, 2H), 2.41 (s, 2H), 0.91 (s, 9H); ¹³C NMR (125 MHz, CDCl₃/MeOH-c¾) δ 159.9, 154.6, 152.4, 150.9, 147.6, 140.4, 133.2, 130.8, 129.3, 127.6, 127.1, 126.2, 1 19.5, 118.9, 114.6, 61.4, 55.6, 49.3, 43.3, 31.3, 27.6; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₉N₆OS₂, 469.1844; found 469.1830; HPLC: method A R_t = 6.84, method B R_t = 7.48.

8-(5-methoxy-2-(lH-pyrazol-3-yl)phenylthio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine [TT-V-140]. lH-Pyrazole-3-boronic acid (26.2 mg, 0.234 mmol) was added to EC102 (30 mg, 0.0585 mmol) and NaHC0₃ (29.5 mg, 0.351 mmol). DMF (1 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H₂0 (0.2 mL) and Pd(PPh₃)₂Cl₂ (8.2 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for 7 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 15 : 1 ) to give 6.2 mg (23%) of TT-V-140. Additionally, 16.4 mg of unreacted EC102 was recovered for an actual yield of 52%. 1H NMR (500 MHz, CDCl₃/MeOH-c¾) δ 8.19 (s, 1H), 7.58 (d, J= 2.1 Hz, 1H), 7.50 (d, J= 8.5 Hz, 1H), 7.03 (d, J= 2.4 Hz, 1H), 6.99 (dd, J = 2.6, 8.6 Hz, 1H), 6.40 (d, J= 2.1 Hz, 1H), 4.42 (t, J= 6.1 Hz, 2H), 3.81 (s, 3H), 3.02 (t, J= 6.1 Hz, 2H), 2.52 (s, 2H), 0.98 (s, 9H); ¹³C NMR (125 MHz, CDCl₃/MeOH-i¾ δ 154.6, 152.3, 150.8, 149.4, 148.6, 148.5, 120.1, 119.2, 114.5, 110.9, 106.0, 102.3, 61.2, 49.1, 42.5, 31.1, 27.5; HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₉N₈OS, 453.2185; found 453.2186; HPLC: method A R_t = 6.61, method B R_t = 6.82.

8- ((2-ethynyl-5-methoxyphenyl)thio)-9-(2-(neopentylamino)ethyl)-9JT-purin-6-amine (PU-WS31). Following the procedure to make PU-WS8, PU-WS31 was obtained from

EC102 as a white solid. 1H NMR (CDC1₃, 500 MHz) δ: 8.35 (s, 1H), 7.46 (d, J= 8.4 Hz, 1H), 6.75 (m, 2H), 5.65 (br, 2H), 4.35 (t, J = 6.3 Hz, 2H), 3.72 (s, 3H), 3.30 (s, 1H), 2.97 (t, J = 6.3 Hz, 2H), 2.31 (s, 2H), 0.87 (s, 9H); MS (ESI) m/z 411.3 (M+H)⁺.

PU-DZ13

Reagents and conditions: (a) RSn(Bu)₃, LiCl, Pd(PPh₃)₄, DMF, 90°C. Scheme 21. Stille coupling of PU-H71 and PU-DZ13.

9-(3-(isopropylamino)propyl)-8-(6-(oxazol-2-yl)benzo [d] [1,3] dioxol-5-ylthio)-9H-purin- 6-amine [DZ4-20]. A mixture of PU-H71 (30 mg, 0.0585 mmol), 2-(tri-«- butylstannyl)oxazole (83.8 mg, 49 μΐ, 0.234 mmol), LiCl (5 mg, 0.117 mmol) and Pd(PPh₃)₄ (6.7 mg, 0.0058 mmol) in DMF (1 mL) was evacuated and back filled with nitrogen. This was repeated four times then the reaction mixture was heated under nitrogen at 90°C for 18 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAC:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 20.8 mg (78%) of DZ4-20. 1H NMR (500 MHz, CDCl₃/MeOH-c¾ δ 8.25 (s, 1H), 7.75 (s, 1H), 7.46 (s, 1H), 7.27 (s, 1H), 6.71 (s, 1H), 6.06 (s, 2H), 4.26 (t, J= 6.9 Hz, 2H), 2.75 (septet, J= 6.3 Hz, 1H), 2.53 (t, J= 6.9 Hz, 2H), 1.98 (m, 2H), 1.06 (d, J= 6.3 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂iH₂₄N₇0₃S, 454.1661 found 454.1650; HPLC: method A R, = 5.77, method B R_t = 5.28.

9-(3-(isopropylamino)propyl)-8-(6-(thiazol-2-yI)benzo[d] [l,3]dioxol-5-ylthio)-9H-purin- 6-amine [DZ4-21]. A mixture of PU-H71 (30 mg, 0.0585 mmol), 2-(tri-«- butylstannyl)thiazole (87.6 mg, 72.4 μΐ, 0.234 mmol), LiCl (5 mg, 0.117 mmol) and

Pd(PPh₃)₄ (6.7 mg, 0.0058 mmol) in DMF (1 mL) was evacuated and back filled with nitrogen. This was repeated four times then the reaction mixture was heated under nitrogen at 90°C for 18 h. Then additional 2-(tri-«-butylstannyl)thiazole (21.9 mg, 18 μΐ, 0.0585 mmol) was added and the reaction mixture was heated under nitrogen at 90°C for an additional 18 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH₂Cl₂:EtOAC:MeOH-NH₃ (7N), 2:2:1 :0.5) to give 17.6 mg (64%) of DZ4-21. 1H NMR (500 MHz, CDCl₃/MeOH-c¾ δ 8.20 (s, 1H), 7.87 (d, J= 3.3 Hz, 1H), 7.45 (s, 1H), 7.44 (d, J= 3.3 Hz, 1H), 6.98 (s, 1H), 6.11 (s, 2H), 4.21 (t, J= 6.9 Hz, 2H), 2.78 (septet, J= 6.3 Hz, 1H), 2.55 (t, J= 6.9 Hz, 2H), 1.98 (m, 2H), 1.09 (d, J= 6.3 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₁H₂₄N₇0₂S₂, 470.1433; found 470.1438; HPLC:

method A R_t = 5.86, method B R_t = 5.66. 2-nuoro-9-(2-(isobutylamino)ethyl)-8-((6-(oxazol-2-yl)benzo[d] [l,3]dioxol-5-yl)methyl)- 9H-purin-6-amine [DZ4-23]. A mixture of PU-DZ13 (20 mg, 0.039 mmol), 2-(tri-#i- butylstannyl)oxazole (55.9 mg, 32.7 μΐ, 0.156 mmol), LiCl (3.3 mg, 0.078 mmol) and Pd(PPh₃)₄ (4.5 mg, 0.0039 mmol) in DMF (1 mL) was evacuated and back filled with nitrogen. This was repeated four times then the reaction mixture was heated under nitrogen at 90°C for 18 h. Then additional LiCl (3.3 mg, 0.078 mmol) and Pd(PPh₃)₄ (4.5 mg, 0.0039 mmol) were added and the reaction mixture was heated under nitrogen at 90°C for an additional 18 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC two times (hexane:CH₂Cl₂:EtOAC:MeOH-NH₃ (7N), 2:2:1:0.5 then CH₂Cl₂:MeOH-NH₃ (7N), 20:1) to give 5.5 mg (31%) of DZ4-23. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 7.68 (s, 1H), 7.53 (s, 1H), 7.12 (s, 1H), 6.84 (s, 1H), 6.07 (s, 2H), 4.74 (s, 2H), 4.41 (t, J- 6.4 Hz, 2H), 3.15 (t, J= 6.4 Hz, 2H), 2.59 (d, J= 6.9 Hz, 2H), 1.88 (m, 1H), 0.96 (d, J= 6.8 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₅FN₇0₃, 454.2003; found 454.1995; HPLC: method A R_t = 6.61, method B R_t - 7.58.

2-nuoro-9-(2-(isobutylamino)ethyl)-8-((6-(thiazol-2-yl)benzo[d] [l,3]dioxol-5-yl)methyl)- 9H-purin-6-amine [DZ4-24]. A mixture of PU-DZ13 (20 mg, 0.039 mmol), 2-(tri-«- butylstannyl)thiazole (58.3 mg, 48.2 μΐ, 0.156 mmol), LiCl (3.3 mg, 0.078 mmol) and Pd(PPh₃)₄ (9 mg, 0.0078 mmol) in DMF (1 mL) was evacuated and back filled with nitrogen. This was repeated four times then the reaction mixture was heated under nitrogen at 90°C for 18 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC two times (hexane:CH₂Cl₂:EtOAC:MeOH-NH₃ (7N), 2:2:1 :0.5 and

CH₂Cl₂:MeOH:AcOH, 20:1 :0.5) to give 10.2 mg (56%) of DZ4-24. 1H NMR (500 MHz, CDCl₃/MeOH-i¾ δ 7.77 (d, J= 3.3 Hz, 1H), 7.36 (d, J= 3.3 Hz, 1H), 7.17 (s, 1H), 6.80 (s, 1H), 6.05 (s, 2H), 4.58 (s, 2H), 4.15 (t, J= 6.6 Hz, 2H), 2.85 (t, J= 6.6 Hz, 2H), 2.35 (d, J = 6.8 Hz, 2H), 1.64 (m, 1H), 0.86 (d, J= 6.8 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₅FN₇0₂S, 470.1774; found 470.1770; HPLC: method A R, = 6.68, method B R_t = 7.79.

PU-H71

Reagents and conditions: (a) alkene (R), Pd(PPh₃)₄, z^'-Pr₂NEt, NMP, 55-100°C.

Scheme 22. Heck coupling of PU-H71.

8-(6-(cyclopent-2-enyl)benzo[d][l,3]dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine [TT-VI-116]. A solution of PU-H71 (30 mg, 0.0585 mmol) in NMP (1 mL) was evacuated and back filled with nitrogen. This was repeated four times, then DIEA (15.1 mg, 21 \ih, 0.117 mmol), cyclopentene (80 mg, 103 \iL, 1.171 mmol) and Pd(PPh₃)₄ (6.8 mg, 0.00586 mmol) were added and the reaction mixture was heated under nitrogen at 100°C for 20 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC two times (CH₂Cl₂:MeOH-NH₃ (7N), 15:1 then CH₂Cl₂:MeOH, 7:3) to give 9.2 mg (35%) of TT-VI-116. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 8.16 (s, 1H), 6.99 (s, 1H), 6.82 (s, 1H), 6.01 (s, 2H), 5.98 (m, 1H), 5.63 (m, 1H), 4.41 (t, J= 6.4 Hz, 2H), 3.39 (m, 1H), 3.34 (septet, J= 6.6 Hz, 1H), 2.95 (t, J= 6.4 Hz, 2H), 2.22-2.52 (m, 5H), 1.50-1.59 (m, 1H), 1.44 (d, J- 6.6 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₃H₂₉N₆0₂S, 453.2073; found 453.2064; HPLC: method A R_t = 6.51, method B R_t = 7.79.

8-(6-(2,5-dihydro-lH-pyrrol-2-yl)benzo [d] [1,3] dioxol-5-ylthio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine [DZ3-141]. A solution of PU-H71 (30 mg,

0.0585 mmol) and N-Boc-2,3-dihydro-lH-pyrrole (19.8 mg, 20.2 μϋ, 0.117 mmol) in NMP (1.5 mL) was evacuated and back filled with nitrogen. This was repeated four times, then DIEA (15.1 mg, 21 μϋ, 0.117 mmol) and Pd(PPh₃)₄ (13.5 mg, 0.01 17 mmol) were added and the reaction mixture was heated under nitrogen at 100°C for 20 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 15:1) and the resulting residue was dissolved into 2 mL of CH₂C1₂:TFA (4:1) and stirred for 1 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (CH₂Cl₂:MeOH-NH₃ (7N), 10: 1) to give 6.0 mg (23%) of DZ3-141. 1H NMR (500 MHz, CDCl₃/MeOH-<¾ δ 8.19 (s, 1H), 6.98 (s, 2H), 6.04 (m, 1H), 6.01 (s, 2H), 5.74 (m, 1H), 5.62 (d, J= 2.0 Hz, 1H), 4.31 (t, J= 6.9 Hz, 2H), 3.81-3.88 (m, 1H), 3.89-3.95 (m, 1H), 2.87 (septet, J= 6.3 Hz, 1H), 2.68 (t, J= 6.7 Hz, 2H), 2.14 (m, 2H), 1.15 (d, J= 6.3 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for

C₂₂H₂₈N₇0₂S, 454.2025; found 454.2046; HPLC: method A R_t = 5.27, method B R_t = 2.72.

8-(6-(2,3-dihydrofuran-2-yl)benzo[d | [l,3]dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)- 9H-purin-6-amine [DZ3-142]. A solution of PU-H71 (30 mg, 0.0585 mmol) in NMP (1.5 mL) was evacuated and back filled with nitrogen. This was repeated four times, then DIEA (15.1 mg, 21 μί, 0.117 mmol), 2,3-dihydrofuran (82 mg, 88 μί, 1.17 mmol) and Pd(PPh₃)₄ (13.5 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 55°C for 20 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC two times (hexane:EtOAc:CH₂Cl₂:MeOH-NH₃ (7N), 2:1 :2:0.5, then CH₂Cl₂:MeOH-NH₃ (7N), 15:1) to give 7.0 mg (26%) of DZ3-142. 1H NMR (500 MHz, CDC1₃) δ 8.23 (s, 1H), 7.06 (s, 1H), 6.96 (s, 1H), 6.43 (m, 1H), 6.01 (s, 2H), 5.94 (dd, J= 8.1, 10.8 Hz, 1H), 5.72 (br s, 2H), 4.93 (m, 1H), 4.35 (t, J= 6.8 Hz, 2H), 2.95-3.55 (m, 2H), 2.70 (t, J= 6.5 Hz, 2H), 2.39-2.47 (m, 1H), 2.22 (m, 2H), 1.25 (d, J= 6.2 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₇N₆0₃S, 455.1865; found 455.1865; HPLC: method A R_t = 6.07, method B R_t = 6.49.

8-(6-(2,3-dihydrofuran-3-yl)benzo[d][l,3]dioxol-5-ylthio)-9-(3-(isopropylamino)propyI)- 9H-purin-6-amine [DZ3-143]. A solution of PU-H71 (30 mg, 0.0585 mmol) in NMP (1.5 mL) was evacuated and back filled with nitrogen. This was repeated four times, then DIEA (15.1 mg, 21 \xL, 0.117 mmol), 2,5-dihydrofuran (82 mg, 88 μί, 1.17 mmol) and Pd(PPh₃)₄ (13.5 mg, 0.0117 mmol) were added and the reaction mixture was heated under nitrogen at 55°C for 20 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC two times (CH₂Cl₂:MeOH-NH₃ (7N), 10:1, then hexane:EtOAc:CH₂Cl₂:MeOH-NH₃ (7N), 2: 1 :2:0.5) to give 5.0 mg (19%) of DZ3-143. 1H NMR (500 MHz, CDCl₃/MeOH-<#) δ 8.18 (s, 1H), 7.02 (s, 1H), 7.00 (s, 1H), 6.55 (m, 1H), 6.04 (s, 2H), 4.99 (m, 1H), 4.64-4.69 (m, 1H), 4.45 (m, 1H), 4.31 (t, J= 6.8 Hz, 2H), 4.05 (dd, J= 6.2, 9.2 Hz, 1H), 3.40 (m, 1H), 2.67 (t, J= 6.4 Hz, 2H), 2.14 (m, 2H), 1.16 (d, J= 6.1 Hz, 6H); HRMS (ESI) m/z [M+H]⁺ calcd. for C₂₂H₂₇N₆0₃S, 455.1865; found 455.1862; HPLC: method A R_t = 6.04, method B R_t = 6.32.

PU-WS23 R= 2-furanyl

PU-WbZI PU-WS24 R= 2-thiophenyl

PU-WS28 R= CCH

Reagents and conditions: (a) RB(OH)₂, PdCl₂(PPh₃)₂, NaHC0₃, H₂0, DMF, 90°C; (b) Cul, PdCl₂(PPh₃)₂, trimethylsilanylacetylene, Et₃N, DMF, 90°C; (c) KOH, MeOH, rt.

Scheme 23. Cross coupling reactions of PU-WS21.

8-(5-(furan-2-yl)-2,3-dihydrobenzofuran-6-ylthio)-9-(2-(neopentylamino)ethyl)-9H- purin-6-amine [PU-WS23]. Following the procedure to make PU-DZ3-4, compound PU- WS23 was obtained as a white solid. ¹HNMR (500 MHz, CDC1₃) δ 8.31 (s, 1H), 7.49 (s, 2H), 6.68 (d, J= 3.4 Hz, 1H), 6.58 (s, 1H), 6.49 (m, IH), 5.60 (br s, 2H), 4.58 (t, J= 8.7 Hz, 2H), 4.25 (m, 2H), 3.22 (t, J= 8.7 Hz, 2H), 2.86 (m, 2H), 2.25 (s, 2H), 0.86 (s, 9H); HRMS (m/z) [M+H]⁺ calcd for C₂₄H₂₉N₆0₂ 465.2073; found 465.2077. 9-(2-(neopentylamino)ethyl)-8-(5-(thiophen-2-yl)-2,3-dihydrobenzofuran-6-ylthio)-9H- purin-6-amine [PU-WS24]. Following the procedure to make PU-DZ2-395, compound PU- WS24 was obtained as a white solid. ¹HNMR (500 MHz, CDC1₃) δ 8.31 (s, 1H), 7.32 (m, 1H), 7.27 (s, 1H), 7.03-7.07 (m, 2H), 6.67 (s, 1H), 5.59 (br s, 2H), 4.58 (t, J= 8.7 Hz, 2H), 4.15 (m, 2H), 3.21 (t, J= 8.7 Hz, 2H), 2.86 (m, 2H), 2.25 (s, 2H), 0.83 (s, 9H); HRMS (m/z): [M+H]⁺ calcd for C₂₄H₂₉N₆0₂ 481.1844; found 481.1825.

8-(5-ethynyl-2,3-dihydrobenzofuran-6-ylthio)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine [PU-WS28]. Following the procedure to make PU-WS8, compound PU-WS28 was obtained as a white solid. ¹HNMR (500 MHz, CDC1₃, δ): 8.33 (s, 1H), 7.36 (s, 1H), 6.59 (s, 1H), 5.70 (br, 2H), 4.58 (t, J= 8.7 Hz, 2H), 4.41 (m, 2H), 3.33 (t, J= 8.7 Hz, 2H), 3.49 (m, 2H), 3.02 (s, 2H), 2.44 (s, 2H), 0.91 (s, 9H); HRMS (m/z): [M+H]⁺ calcd for C₂₂H₂₇N₆OS 423.1967; found 423.1968.

S24-7 S24-8

Reagents and conditions: (a) Ac₂0, AcOH, rt; (b) IC1, CH₂C1₂, AcOH, rt; (c) NaOH, EtOH, H₂0, reflux; (d) paraformaldehyde, NaBH₃CN, MeOH, 50°C; (e) 8-mercaptoadenine, neocuproine,

Cul, NaOtBu, DMF, 115°C; (f) Cs₂C0₃, 1,3-dibromopropane or 1,2-dibromoethane, DMF, rt; (g) amine, DMF, rt.

Scheme 24.

Similarly,

Scheme 25. Synthesis of various bromides required for alkylation of the purine skeleton.

5 or 6- 5 or 6- membered ring membered ring

Table 1A

No. Name

lA-1 PU-WS10

8-((6-iodo-2,3-dihydrobenzofuran-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1A-2 8-((6-iodo-2,3-dihydrobenzofiiran-5-yl)thio)-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine

1A-3 l-(6-amino-8-(6-iodo-2,3-dihydrobenzofuran-5-ylthio)-9H-purin-9-yl)-3- (tert-butylamino)propan-2-ol

1A-4 2-fluoro-8-((6-iodo-2,3-dihydrobenzofliran-5-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

1A-5 2-cWoro-8-((6-(furan-2-yl)-2,3-dihydrobenzofuran-5-yl)methyl)-9-(3^

(isopropylamino)propyl)-9H-purin-6-amine

1A-6 2-fluoro-8-((6-iodo-2,3 -dihydrobenzofuran-5 -yl)methyl)-9-(3 - (isopropylamino)propyl)-9H-purin-6-amine

1A-7 8-((6-(furan-2-yl)-2,3-dihydrobenzofuran-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1A-8 8-((6-ethynyl-2,3-di ydrobenzofuran-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine ,

1A-9 5-((6-amino-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)thio)-2,3- dihydrobeiizofuran-6-carbonitrile

lA-10 8-((6-(aziridin-l-yl)-2,3-dihydroberizofe

(neopentylamino)ethyl)-9H-purin-6-amine

lA-11 8-((6-iodo-2,3-dihydrobenzofuran-5-yl)thio)-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine

1A-12 3 -(2-(6-amino-8-(6-(5 -methylfuran-2-yl)-2,3 -dihydrobenzofuran-5 - ylthio)-9H-purin-9-yl)ethyl)piperidine- 1 -sulfonamide

1A-13 l-(3-(2-(8-(6-(lH-pyrazol-3-yl)-2,3-dihydroberizofuran-5-ylthio)-6- amino-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

1A-14 4-(3-(6-amino-8-(6-emynyl-2,3-dmydroberizofuran-5-ylt iio)-9H-purin-9- yl)propyl)piperidine- 1 -carbaldehyde

1A-15 l-(3-(2-(6-amino-2-fluoro-8-((6-(furan-2-yl)-2,3-dihydroberizofuran-5- yl)methyl)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

1A-16 N-(2-((2-(6-arnino-8-((6-(furan-2-yl)-2,3-dihydroberizofuran-5-yl)to

9H-purin-9-yl)ethyl)amino)ethyl sulfamide

1A-17 3-((2-(6-amino-8-((6-(furan-2-yl)-2,3-dihydroberizofuran-5-yl)tW

purin-9-yl)ethyl)amino)-N-hydroxypropanamide

1A-18 2-fluoro-8-((6-iodo-2,3-dihydroberizofuran-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine A-19 2-cMoro-8-((6-iodo-2,3-dihydrobenzofuran-5-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

A-20 9-(2-aminoethyl)-2-fluoro-8-((6-iodo-2,3-(lihydrobenzofuran-5- yl)methyl)-9H-purm-6-amine

A-21 9-(3-aminopropyl)-8-((6-iodo-2,3-(lihydrobenzofuran-5-yl)tMo)-9H- purin-6-amine

A-22 9-(2-aminoethyl)-8-((6-iodo-2,3-dihydrobenzoi iran-5-yl)thio)-9H-purin- 6-amine

A-23 9-(3-(tert-butylamino)propyl)-8-((6-iodo-2,3-dihydrobenzofuran-5- yl)thio)-9H-purin-6-amine

A-24 l-(6-amino-8-((6-iodo-2,3-dihydrobenzoi iran-5-yl)tWo)-9H-purin-9-yl)- 3 -(isopropylamino)propan-2-ol

A-25 l-(3-(6-amino-2-fluoro-8-((6-iodo-2,3-dihydrobenzofuran-5-yl)methyl)- 9H-purin-9-yl)propyl)pyrrolidin-3-one

A-26 l-(3-(6-amino-8-(6-iodo-2,3-(lihydrobenzoi iran-5-yltMo)-9H-purin-9- yl)propyl)pyrrolidin-3 -one

A-27 5-(6-amino-8-(6-iodo-2,3-dihydrobenzoi iran-5-yltiiio)-9H-purin-9- yl)pentane- 1 -sulfonamide

A-28 l-(6-amino-2-fiuoro-8-((6-iodo-2,3-dihydrobenzoiuran-5-yl)methyl)-9H- purin-9-yl)-3-(isopropylamino)propan-2-ol

A-29 8-((6-emynyl-2,3-dihydrobenzofuran-5-yl)methyl)-2-fiuoro-9-(5- (methylsulfonyl)pentyl)-9H-purin-6-amine

A-30 2-fluoro-8-((6-iodo-2,3-dihydrobenzofuran-5-yl)methyl)-9-(2-(l- (memylsulfonyl)pyrrolidin-3-yl)ethyl)-9H-purin-6-amine

A-31 N-(4-(6-amino-2-fiuoro-8-((6-iodo-2,3-dihydrobenzoiuran-5-yl)methyl)- 9H-purin-9-yl)butyl)methanesulfonamide

A-32 l-(6-amino-8-((6-ethynyl-2,3-dihydrobenzofuran-5-yl)metliyl)-2-fluoro- 9H-purin-9-yl)-3-(isopropylaniino)propan-2-ol

A-33 6-(6-amino-8-((6-ethynyl-2,3-dihydrobenzoiuran-5-yl)metliyl)-2-fiuoro- 9H-purin-9-yl)hexanamide

A-34 l-(4-(2-(6-amino-8-((6-et ynyl-2,3-dihydrobenzofuran-5-yl)methyl)-2- fluoro-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

A-35 8-((6-ethynyl-2,3-dihydrobenzofuran-5-yl)methyl)-

2-fluoro-9-(2-(l-(mefliylsulfonyl)piperidin-3- yl)ethyl)-9H-purin-6-amine

9-(3-(isopropylamino)propyl)-8-((6-(5- methyliuran-2-yl)-2,3-dihydrobenzofuran-5- yl)thio)-9H-purin-6-amine

9-(3-(tert-butylamino)propyl)-8-((6-(5-methylfuran- 2-yl)-2,3-dihydrobenzoiuran-5-yl)thio)-9H-purin-6- amine

9-(3 -(tert-butylamino)propyl)-8 -((6- (dimethylamino)-2,3-di ydrobenzofuran-5-yl)thio)- 9H-purin-6-amine

l-(4-(2-(6-amino-8-((6-(dimethylamino)-2,3- dihydrobenzofuran-5-yl)thio)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone

1A-40 8-((6-(dimethylamino)-2,3-dihydrobenzofuran-5-yl)thio)-9-(2-(l- (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine

1A-41 4-(3-(6-amino-8-(6-(aziridin-l-yl)-2,3-dihydrobenzofuran-5-ylthio)-9H- purin-9-yl)propyl)piperidine- 1 -carbaldehyde

1A-42 8-((6-(furan-2-yl)-2,3-dihydrobenzofuran-5-yl)thio)-9-(2-(l- (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine

1A-43 8-((6-(di methylamino)-2,3-dihydrobenzofuran-5-yl)methyl)-2-fluoro-9-(2- (isobuty amino)ethyl)-9H-purin-6-arnine

1 LA-44 8-((6-(dimethylamino)-2,3-dihydrobenzoiuran-5-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purih-6-amine

1A-45 9-(3-(tert-butylamino)propyl)-8-((6-(oxazol-2-yl)-2,3-dihydrobenzoiuran-5- yl)thio)-9H-purin-6-amine

1A-46 l-(3-(2-(6-amino-8-(6-(oxazol-2-yl)-2,3-dihydrobenzofiaran-5-ylthio)-9H-purin- 9-yl)ethyl)piperidin-l -yl)ethanone

1A-47 4-(3-(6-amino-8-(6-(oxazol-2-yl)-2,3-dihydrobenzoftiran-5-ylthio)-9H-purin-9- yl)propyl)piperidine- 1 -carbaldehyde

1A-48 9-(2-(l-(methylsulfonyl)piperidin-3-yl)ethyl)-8-((6-(oxazol-2-yl)-2,3- dihydrobenzofuran-5-yl)thio)-9H-purin-6-amine

1A-49 l-(2-(3-(6-armno-8-(6-iodo-2,3-dihydrobenzofuran-5-ylthio)-9H-purin-9- yl)propyl)pyrrolidin- 1 -yl)ethanone

1A-50 9-(3-(tert-butylamino)propyl)-8-((6-(5-methyloxazol-2-yl)-2,3- dihydrobenzofUran-5 -yl)thio)-9H-purin-6-amine

1A-51 9-(3-(tert-bu1ylarrdno)propyl)-8-((6-(thiazol-2-yl)-2,3-dihydrobenzofuran-5- yl)thio)-9H-purin-6-amine

1A-52 9-(3-(tert-butylamino)propyl)-8-((6-(5-methylthiazol-2-yl)-2,3- dihydrobenzofuran-5-yl)thio)-9H-purin-6-arnine

Table IB

No. Name

lB-1 PU-WS9

8-((5-iodo-2,3-dihydrobenzofuran-6-yl)thio)-9-(2-(isobu1ylamino)ethyl)-9H-puri 6-amine

1B-2 PU-WS4

8-((5-iodo-2,3-dihydrobenzofiaran-6-yl)thio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine 1B-3 PU-WS17

2-fluoro-8-((5-iodo-2,3-dihydrobenzofiiran-6-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-pnrin-6-amine

1B-4 PU-WS18

2-fluoro-8-((5-iodo-2,3-dihydrobenzofiiran-6-yl)methyl)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1B-5 2-chloro-8-((5-iodo-2,3-dihydrobenzofiiran-6-yl)methyl)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1B-6 8-((5-(furan-2-yl)-2,3-dihydrobenzofuran-6-yl)thio)-9-(3-(isopropylamino)propyl)- 9H-purin-6-amine

1B-7 8-((5-(dimethylamino)-2,3-dihydrobenzofL-ran-6-yl)methyl)-2-fluoro-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

1B-8 8-((5-(lH-pyrazol-3-yl)-2,3-dihydrobenzofiiran-6-yl)thio)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

1B-9 8-((5-Gyclopentyl-2,3-dihydrobenzofiiran-6-yl)thio)-9-(3-(isopropylamino)propyl)- 9H-purin-6-amine

lB-10 8 -((5 -ethynyl-2 ,3 -dihydrobenzofur an-6 -yl)methyl) -2 -fluoro-9 -(2 - (isobutylamino)ethyl)-9H-purin-6-amine

lB-11 6-((6-amino-2-fluoro-9-(3-(isopropylamino)propyl)-9H-p\irin-8-yl)methyl)-2,3- dihydrobenzofuran-5-carbonitrile

1B-12 2-chloro-9-(3-(isopropylamino)propyl)-8-((5-(5-methylfuran-2-yl)-2,3- dihyckoberizofiiran-6-yl)methyl)-9H-purm-6-amine

1B-13 4-(2-(6-amino-8-((5-ethynyl-2,3-dihydrobenzofuran-6-yl)thio)-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

1B-14 l-(4-(2-(6-amino-2-fluoro-8-((5-(furan-2-yl)-2,3-dihydrobenzofuran-6-yl)methyl)- 9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

1B-15 N 2 (2-(6-amino-8-((5 fL-ran-2-yl)-2,3-dihydrobenzofLiran-6-yl)thio)-9H-purin

9-yl)ethyl)amino)ethyl)sulfamide

1B-16 3 -((2 -(6-amino-8 -((5 -ethynyl-2,3 -dihydrobenzofur an-6-yl)thio)-9H-purin-9- yl)ethyl)arnino)-N-hydroxypropanamide

1B-17 9-(2-(isobu1ylamino)ethyl)-8-((5-(thiophen-2-yl)-2,3-dihydrobenzofuran-6-yl)thi

9H-purin-6-amine

1B-18 3-(3-(6-ann^o-8-(5-iodo-2,3-dihydrobenzofuran-6-ylthio)-9H-purin-9-yl)propyl)-2- oxoimidazolidine- 1 -carbaldehyde

1B-19 2-chloro-8-((5-ethynyl-2,3-dihydrobenzofuran-6-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

1B-20 6-((6-amino-2-chloro-9-(3-(isopropylarnino)propyl)-9H-purin-8-yl)methyl)-2,3- dihydrobenzofuran-5-carbonitrile

1B-21 2-chloro-8-((5-(dimethylamino)-2,3-dihydrobenzofuran-6-yl)methyl)-9-(2- (neopen1ylarnino)ethyl)-9H-piirin-6-amine

1B-22 8-((5-cyclopen1yl-2,3-dihydrobenzofuran-6-yl)thio)-2-fluoro-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1B-23 1 -(3 -(6-amino-8-(5 -iodo-2 ,3 -dihydrobenzofuran-6-ylthio)-9H-purin-9- yl)propyl)pyrrolidin-3 -one

1B-24 PU-WS21

8-((5-iodo-2,3-dihydrobenzofuran-6-yl)thio)-9-(2-(neopen1ylamino)ethyl)-9H- purin-6-amine

1B-25 PU-WS22

2-fluoro-8-((5-iodo-2,3-dihydrobenzofuran-6-yl)methyl)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

1B-40 8-((5-ethynyl-2,3-dihydrobenzofuran-6-yl)thio)-9-(2-(l-(methylsulfonyl)piperidm 3-yl)ethyl)-9H-purin-6-amine

1B-41 5 -(6-amino-8 -(5 -ethynyl-2,3 -dihyckobenzofuran-6-ylthio)-9H-purin-9-yl)pentane- 1 -sulfonamide

1B-42 l 4-(2-(6-amino-8-((5-(furan-2-yl)-2,3-dihydrobenzofuran-6-yl)thio)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone

1B-43 8-(5-(5-methylfuran-2-yl)-2,3-dihyckobenzofuran-6-ylthio)-9-(2-( 1 - (methylsulfonyl)piperidin-3 -yl)ethyl)-9H-purin-6-amine

1B-44 2-(3-(6-amino-8-(5-(5-methylfuran-2-yl)-2,3-dihyckobenzofuran-6-ylthio)-9H- purin-9-yl)propyl)pyrrolidine- 1 -carbaldehyde

1B-45 N-(2-(6-amino-8-((5-ethynyl-2,3-dihydrobenzofuran-6-yl)thio)-9H-purin-9- .

yl)ethyl) N'-methyl-sulfuric diamide

1B-46 9-(3-(tert-bu1ylamino)propyl)-8-((5-(oxazol-2-yl)-2,3-dihydrobenzofuran-6- yl)thio)-9H-purin-6-amine

1B-47 9-(3-(tert-bu1ylamino)propyl)-8-((5-ethynyl-2,3-dihydrobenzofuran-6-yl)thio)-9H- purin-6-amine

1B-48 9-(3-(tert-bu1ylamino)propyl)-8-((5-(dimethylamino)-2,3-dihydrobenzofuran-6- yl)thio)-9H-purin-6-amine

lB-49 l-(6-amino-8-((5-iodo-2,3-dihyckobenzofuran-6-yl)thio)-9H-purin-9-yl)-3- (isopropylamino)propan-2-ol

1B-50 l-(4-(2-(6-amino-8-((5-(oxazol-2-yl)-2,3-dihydrobenzofuran-6-yl)thio)-9H-purin- 9-yl)ethyl)piperidin- 1 -yl)ethanone

1B-51 9-(2-(l-(methylsulfonyl)piperidin-3-yl)ethyl)-8-((5-(oxazol-2-yl)-2,3- dihydrobenzofuran-6-yl)thio)-9H-purin-6-amine

1B-52 3-(3-(6-amino-8-(5-iodo-2,3-dihydrobenzofuran-6-ylthio)-9H-purin-9- yl)propyl)pyrrolidine- 1 -sulfonamide

1B-53 9-(3-(tert-butylamdno)propyl)-8-((5-(5-methyloxazol-2-yl)-2,3-dihyckobenzofiaran- 6-yl)thio)-9H-purin-6-amine

1B-54 9-(3-(tert-bu1ylamino)propyl)-8-((5-(thiazol-2-yl)-2,3-dihydrobenzofuran-6- yl)thio)-9H-purin-6-amine

1B-55 9-(3-(tert-bu1ylamino)propyl)-8-((5-(5-methylthiazol-2-yl)-2,3-dihydrobenzofuran- 6-yl)thio)-9H-purin-6-amine

1B-56 6-(6-amino-8-(5-iodo-2,3-dihydrobenzofuran-6-ylthio)-9H-purin-9-yl)hexanamide

1B-57 5-(6-amino-8-(5-iodo-2,3-dihydrobenzofuran-6-ylthio)-9H-purin-9-yl)pentane-l- sulfonamide

1B-58 3-(6-amdno-8-(5-ethynyl-2,3-dihydrobenzofuran-6-ylthio)-9H-purin-9-yl)propyl sulfamate

Table 1C

No. Name

lC-1 8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

lC-2 8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine

lC-3 8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-piirin-6-amine

lC-4 2-fluoro-8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

lC-5 2-chloro-8^(6^furan-2-yl)-2,3-dihydrobenzo[b]thiophen-5-yl)methyl)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

lC-6 8 (6-(lH-imidazol-4-yl)-2,3-dihydrobenzo[b]thiophen-5-yl)methyl)-2-fluoro-9- (3-(isopropylamino)propyl)-9H-purin-6-amine

lC-7 8-((6-(furan-2-yl)-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

lC-8 8-((6-ethynyl-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

lC-9 5-((6-amino-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)thio)-2,3- dihydrobenzo[b]thiophene-6-carbonitrile

lC-10 8-((6-(aziridin-l-yl)-2,3-dihydrobenzo[b]thiophen-5-yl)methyl)-2-fluoro-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

lC-11 8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine

lC-12 l-(4-(2-(6-aimno-8-((6-(furan-2-yl)-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9H- purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

lC-13 4-(2-(8-((6-(lH-p razol-3-yl)-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-6-amino- 9H-purin-9-yl)ethyl)piperidine- 1 -carbaldehyde

lC-14 4-(2-(6-amino-8-((6-ethynyl-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

lC-15 l-(4-(2-(6-armno-2-fluoro-8-((6-(furan-2-yl)-2,3-dihydrobenzo[b]thiophen-5- yl)methyl)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

lC-16 N-(2-((2-(6-armno-8-((6-(iuran-2-yl)-2,3-dihydrobenzo[b]thiophen-5-yl)thio)- 9H-purin-9-yl)ethyl)amino)ethyl)sulfamide

lC-17 3-((2-(6-amino-8 (6-(furan-2-yl)-2,3-dihydroberLzo[b]thiophen-5-yl)thio)-9H- purin-9-yl)ethyl)amino)-N-hydroxypropanamide

lC-18 9-(3-aminopropyl)-8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9H-purin- 6-amine

lC-19 9-(2-aminoethyl)-2-fluoro-8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)methy

9H-purin-6-amine

lC-20 9-(2-arrunoethyl)-8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)thio)-9H-purin-6- amine

lC-21 9-(3-(tert-butylarnino)propyl)-8-((6-iodo-2,3-dihydrobenzo[b]thiophen-5-yl)thio)- 9H-purin-6-amine

Table ID

No. Name

lD-1 8 -((5 -iodo-2,3 -dihydrobenzo [b]thiophen-6-yl)thio)-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine

1D-2 8-((5-iodo-2,3-dihydrobenzo[b]thiophen-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1D-3 2-fluoro-8-((5-iodo-2,3-dihydrobenzo[b]thiophen-6-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

1D-4 2-fluoro-8-((5-iodo-2,3-dihydrobenzo[b]thiophen-6-yl)methyl)-9-(3- (isopropylarnino)propyl)-9H-purin-6-amine

1D-5 8-((5-iodo-2,3-dihydrobenzo[b]thiophen-6-yl)thio)-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine

1D-6 8-((5-(furan-2-yl)-2,3-dihydrobenzorbTthiophen-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1D-7 8-((5-(dimethylamino)-2,3-dihydrobenzo[b]thiophen-6-yl)methyl)-2-fluoro-9- (2-(neopentylamino)ethyl)-9H-purin-6 -amine

1D-8 8-((5-( 1 H-pyrazol-3 -yl)-2,3 -dihydrobenzo[b]thiophen-6-yl)thio)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

1D-9 8-((5-cyclopentyl-2,3-dihydrobenzo[b]thiophen-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

lD-10 8-((5-ethynyl-2,3-dihydrobenzo[b]thiophen-6-yl)methyl)-2-fluoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

lD-11 6-((6-amino-2-fluoro-9-(3-(is0propylamino)propyl)-9H-purin-8-yl)methyl)-2,3- dihydrobenzo [b] thiophene-5 -carbonitrile

1D-12 2-chloro-8-((5-(furan-2-yl)-2,3-dihydrobenzo[b]thiophen-6-yl)methyl)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1D-13 4-(2-(6-amino-8-((5 -ethynyl-2,3 -dihydrobenzo[b]thiophen-6-yl)thio)-9H-purin- 9-yl)ethyl)piperidine- 1 -carbaldehyde

1D-14 l-(4-(2-(6-amino-2-fluoro-8-((5-(furan-2-yl)-2,3-dihydrobenzo[b]thiophen-6- yl)methyl)-9H-purin-9-yl)ethyl)piperidin-l-yl)ethanone

1D-15 N-(2-((2-(6-amino-8-((5-(furan-2-yl)-2,3-dihydrobenzo[b]thiophen-6-yl)thio)- 9H-purin-9-yl)ethyl)amino)ethyl)sulfamide

1D-16 3-((2-(6-amino-8-((5-ethynyl-2,3-dihydrobenzo[b]thiophen-6-yl)thio)-9H- purin-9-yl)ethyl)amino)-N-hydroxypropanamide

1D-17 2-chloro-8-((5-iodo-2,3-dihydrobenzo[b]thiophen-6-yl)methyl)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1D-18 9-(3-aminopropyl)-8-((5-iodo-2,3-dihydrobenzo[b]thiophen-6-yl)thio)-9H- purin-6-amine

1D-19 9-(2-aniinoethyl)-2-fluoro-8-((5-iOdo-2,3-dihydrobenzo[b]thiophen-6- yl)methyl)-9H-purin-6-amine

1D-20 9-(2-aminoethyl)-8-((5-iodo-2,3-dihydrobenzo[b]thiophen-6-yl)thio)-9H-purin- 6-amine

1D-21 9-(3-(tert-butylamino)propyl)-8-((5-iodo-2,3-dihydrobenzo[b]thiophen-6- yl)thio)-9H-purin-6-amine

Table IE

No. Name

lE-1 l-(6-amino-8-(6-iodo-2,3-dihydro H-inden-5-ylthio)-9H-purin-9-yl)-3-(tert- butylamino)propan-2 -ol

1E-2 PU-WS26

8-((6-iodo-2,3-dihydro^H-inden-5-yl)thio)-9 2 isobu1ylamino)ethyl)-9H-piirin-6- amine

1E-3 1 -(3 -(6-amino-8-(6 -iodo-2 ,3 -dihydro- 1 H-inden-5 -ylthio)-9H-purin-9- yl)propyl)pyrrolidin-3 -one

1E-4 2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine

1E-5 2-chloro-8-((6-(furan-2-yl)-2,3-dihydro-lH-inden-5-yl)methyl)-9-(3- (isopropylarnino)propyl)-9H-purin-6-arnine

1E-6 2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9-(3-(isopropylarnino)propyl)- 9H-purin-6-amine

1E-7 8-((6-(fiiran-2 -yl)-2 ,3 -dihydro- 1 H-inden-5 -yl)thio)-9-(3 -(isopropylamino)propyl)-9H- purin-6-amine

1E-8 8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)thio)-9-(3-(isopropylamino)propyl)-9H-purin- 6-amine

1E-9 6-((6-amino-9-(3-(isopropylarnino)propyl)-9H-p jrin-8-yl)thio)-2,3-dihydro-lH-indene- 5-carbonitrile

lE-10 8-((6-(azetidin-l-yl)-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9-(2- (neopentylarmno)ethyl)-9H-pimn-6-amine

lE-11 9-(3-(isopropylarnino)propyl)-8-((6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-yl)thio)-9H- purin-6-amine

1E-12 l-(3-(2-(6-amino-8-(6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone

1E-13 3 2 8-(6 lH-pyrazol-3-yl)-2,3-dmydro H-inden-5-ylthio)-6-amino-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

1E-14 l-(3-(2-(6-amino-8-(6-ethynyl-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone

1E-15 2-fluoro-9-(3-(l-(methylsulfonyl)pyrrolidin-3-yl)propyl)-8-((6-(oxazol-2-yl)-2,3- dihydro-lH-inden-5-yl)met yl)-9H-piirin-6-amine

1E-16 N-(2-((2-(6-amino-8-((6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-yl)thio)-9H-purin-9- yl)ethyl)amino)ethyl)sulfamide

1E-17 3-(2 6-amino-8-(6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)ethylamino)-N-hydroxypropanamide

1E-18 l-(3-(3-(6-amino-8-(6-iodo-2,3-dihydro-lH-inden-5-ylthio)-9H-p rin-9- yl)propyl)pyrrolidin- 1 -yl)ethanone

1E-19 l-(3-(3 6-amino-8 6-ethynyl-2,3-dihydro H-inden-5-ylthio)-9H-p rin-9- yl)propyl)pyrrolidin- 1 -yl)ethanone

1E-20 2-chloro-8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1E-21 PU-WS25

8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(neopen1ylamino)ethyl)-9H-purin-6- amine

1E-22 PU-WS27

8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(neopen1ylamino)ethyl)-9H-purin- 6-amine

1E-23 PU-WS29

8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9-(3-(isopropylamino)propyl)-9H-purin-6- amine

1E-24 9-(3 -aminopropyl)-8 -((6-iodo-2,3 -dihydro- 1 H-inden-5 -yl)thio)-9H-purin-6-amine

1E-25 9-(2-aminoethyl)-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9H^urin-6-amine

1E-26 9-(3-(tert-bu1ylatmno)propyl)-8-((6 odo-2,3-dihydro-lH nden-5-yl)thio)-9H-purin-6- amine

1E-27 9-(3-(isopropylamino)propyl)-8-((6-(5-methyloxazol-2-yl)-2,3-dihydro-lH-inden-5- yl)thio)-9H-purin-6-amine

1E-28 l-(4-(3-(6-ainino-8-(6-(dimethylainino)-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)propyl)piperidin- 1 -yl)ethanone

1E-29 l-(3-(2-(6-amino-2-fluoro-8-((6-(4-methylthiazol-2-yl)-2,3-dihydro-lH-inden-5- yl)methyl)-9H-purin-9-yl)ethyl}piperidin- 1 -yl)ethanone

1E-30 8-((6-(5-methyloxazol-2-yl)-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(l- (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine

1E-31 9-(3-aminopropyl)-8-((6-(5-methyloxazol-2-yl)-2,3-di ydro-lH-inden-5-yl)thio)-9H- purin-6-amine

lE-32 9-(3 tert-bu1ylamino)propyl)-2-fluoro-8-((6-(4-methylthiazol-2-yl)-2,3-dihydro-lH- inden-5-yl)methyl)-9H-purin-6-amine

1E-33 8-((6-(lH-pyrazol-3-yl)-2,3-dihydro-lH-inden-5-yl)methyl)-9-(3-(tert- bu1ylamino)propyl)-2-fluoro-9H-piirin-6-an ine

1E-34 8-(6-(aziridin-l-yl)-2,3-dihydro-lH-inden-5-ylthio)-9-(3-(l-(methylsulfonyl)pyrrolidin- 3-yl)propyl)-9H-purin-6-amine

1E-35 l-(3-(6-amino-2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9H-purin-9- yl)propyl)pyrrolidin-3 -one

1E-36 8-((6-(5-methyloxazol-2-yl)-2,3-dihydro-lH-inden-5-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

1E-37 l-(6-amino-8-((6-(5-methyloxazol-2-yl)-2,3-dihydro-lH-inden-5-yl)thio)-9H-purin-9- yl)-3-(isopropylamino)propan-2-ol

1E-38 5 -(6-armno-8 -(6-iodo-2 ,3 -dihyclro- 1 H-ind

1 -sulfonamide

1E-39 5 -(6-amino-8 -(6-iodo-2 ,3 -dihydro- 1 H-inden-5 -ylthio)-9H-purin-9-yl)pentane- 1 - sulfonamide

1E-40 5-(6-amino-8-(6-ethynyl-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9-yl)pentane-l- sulfonamide

1E-41 l-(2-(4-(6-amino-8-(6-(5-methylfbran-2-yl)-2,3-dihydro-lH-inden-5-ylthio)-9H-purin- 9-yl)butyl)pyrrolidin- 1 -yl)ethanone

1E-42 l-(3-(6-amino-8-(6-iodo-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)propyl)pyrrolidin-3 -ol

1E-43 6-(6-amino-8-(6-iodo-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9-yl)hexanamide

1E-44 l-(3-(3-(6-amino-2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9H-purin-9- yl)propyl)pyrrolidin- 1 -yl)ethanone

1E-45 5-(6-amino-2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9H-purin-9-yl)-N- methylpentane- 1 -sulfonamide

1E-46 5 -(6-amino-2-fluoro-8 -((6-iodo-2 ,3 -dihydro- 1 H-inden-5 -yl)methyl)-9H-purin-9- yl)pentane- 1 -sulfonamide

lE-47 9-(3 -(tert-butylamino)propyl)-2 -fluoro-8-((6-iodo-2 ,3 -dihydro- 1 H-inden-5 -yl)methyl)- 9H-purin-6-amine

1E-48 2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9-(2-(neopentylamino)ethyl)- 9H-purin-6-amine

1E-49 l-(4-(3-(6-amino-8-(6-ethynyl-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)propyl)piperidin- 1 -yl)ethanone

1E-50 l-(3-(2-(6-amino-8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9H-purin- 9-yl)ethyl)piperidin-l -yl)ethanone

1E-51 9-(3-(tert-butylamino)propyl)-8-(6-ethynyl-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-6- amine

1E-52 9-(3-(tert-butylamino)propyl)-8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2- fluoro-9H-purin-6-amine

1E-53 6-(6-amino-8 -((6-ethynyl-2 ,3 -dihydro- 1 H-inden-5 -yl)methyl)-2 -fluoro-9H-purin-9- yl)hexanamide

1E-54 l-(3-(6-amino-8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9H-purin-9- yl)propyl)pyrrolidin-3 -one

1E-55 4-(6-amino-8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9H-purin-9- yl)butane- 1 -sulfonamide

1E-56 8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

1E-57 8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

1E-58 l-acetyl-3-(3-(6-amino-8-(6-ethynyl-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)propyl)imidazolidin-2-one

1E-59 9-(3-(tert-butylamino)propyl)-8-(6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-ylthio)-9H- purin-6-amine

1E-60 9-(3 -(tert-butylamino)propyl)-8 -(6-(5 -methyloxazol-2 -yl)-2,3 -dihydro- 1 H-inden-5 - ylthio)-9H-purin-6-amine

1E-61 8-(6-( 1 H-pyrazol-3 -yl)-2 ,3 -dihydro- 1 H-inden-5 -ylthio)-9 -(3 -(tert-butylamino)propyl)- 9H-purin-6-amine

1E-62 l-(3-(2-(6-amino-8-(6-(5-methyloxazol-2-yl)-2,3-dihydro-lH-inden-5-ylthio)-9H-purin- 9-yl)ethyl)piperidin- 1 -yl)ethanone lE-63 6-(6-amino-8-(6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)hexanamide

1E-64 l-(3-(6-amino-8-(6-(4-methyloxazol-2-yl)-2,3-dihydro^^

yl)propyl)pyrrolidin-3 -one

1E-65 6-(6-amino-2-fluoro-8-((6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-yl)methyl)-9H-purin- 9-yl)hexanamide

1E-66 l 3 6-amino-2-fluoro-8 (6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-yl)methyl)-9H- purin-9-yl)propyl)pyrrolidin-3-one

1E-67 5 -(6-amino-2 -fluoro-8 -((6-(oxazol-2 -yl)-2, 3 -dihydro- 1 H-inden-5 -yl)methyl)-9H-purin- 9-yl)pentane-l -sulfonamide

1E-68 8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(l-methylpiperidin-2-yl)ethyl)-9H- purin-6-amine

1E-69 8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(l-methylpiperidin-3-yl)ethyl)-9H- purin-6-amine

1E-70 8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(l-(methylsulfonyl)piperidin-3- yl)ethyl)-9H-purin-6-amine

1E-71 3-(2-(6-amino-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9H-purin-9- yl)ethyl)piperidine- 1 -sulfonamide

1E-72 2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9-(2-(l-methylpiperidin-2- yl)ethyl)-9H-purin-6-amine

1E-73 2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9-(2-(l-methylpiperidin-3- yl)ethyl)-9H-purin-6-amine

1E-74 2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9-(2-(l- (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine

1E-75 3-(2-(6-amino-2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9H-purin-9- yl)ethyl)piperidine- 1 -sulfonamide

1E-76 9-(3 -(tert-butylamino)propyl)-2 -fluoro-8-((6-iodo-2 ,3 -dihydro- 1 H-inden-5 -yl)methyl)- 9H-purin-6-amine

1E-77 8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(l-methylpiperidin-2-yl)ethyl)-9H- purin-6-amine

1E-78 8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(l-methylpiperidin-3-yl)ethyl)-9H- purin-6-amine

1E-79 3-(2-(6-amino-8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9H-purin-9- yl)ethyl)piperidine- 1 -sulfonamide

1E-80 8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)thio)-9-(2-(l-(methylsulfonyl)piperidin-3- yl)ethyl)-9H-purin-6-amine

1E-81 8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9-(2-(l-methylpiperidin-2- yl)ethyl)-9H-purin-6-amine

1E-82 8-((6-ethynyl-2,3-dihydro-lH-inden-5-yl)methyl)-2-fluoro-9-(2-(l-methylpiperidin-3- yl)ethyl)-9H-purin-6-amine

1E-83 9-(3-(tert-butylamino)propyl)-8-(6-(4-methylthiazol-2-yl)-2,3-dihydro-lH-inden-5- ylthio)-9H-purin-6-amine

1E-84 2-fluoro-9-(2-(l-methylpiperidin-2-yl)ethyl)-8-((6-(oxazol-2-yl)-2,3-dihydro-lH-inden- 5-yl)methyl)-9H-purin-6-amine

1E-85 2-fluoro-9-(2-(l-methylpiperidin-3-yl)ethyl)-8-((6-(oxazol-2-yl)-2,3-dihydro-lH-inden- 5 -yl)methyl)-9H-purin-6-amine

Table IF

No. Name lF-1 8-((6-iodoindolin-5-yl)thio)-9-(2-(isobu1ylamino)ethyl)-9H^iirin-6-amine

1F-2 2-fluoro-8-((6-iodoindolin-5-yl)methyl)-9-(2-(^

1F-3 8-((6-(lH^yrazol-3-yl)mdolin-5-yl)thio)-9-(2-(isobu1ylamino)ethyl)-9H-purin-6-amine

1F-4 8-((6-ethynylindolin-5-yl)thio)-9-(2-(isobu1ylamino)ethyl)-9H^urin-6-amine

1F-5 8-((6-(lH-pyrrol-3-yl)indolin-5-yl)methyl)-2-fluoro-9-(2-(isobu1ylamino)ethyl)-9H- purin-6-amine

1F-6 5-((6-amino-9-(3-(isopropylamino)propyl)-9H-piirin-8-yl)thio)indoline-6-carbonitrile

1F-7 8-((6-(furan-2-yl)-l-methylindolin-5-yl)thio)-9-(3-(isopropylamino)propyl)-9H-purin- 6-amine

1F-8 8-((6-cyclobut l-l-methylindolin-5-yl)methyl)-2-fluoro-9-(2-(isobut lamino)ethyl)- 9H-purin-6-amine

1F-9 l-(5-((6-amino-2-fluoro-9-(2-(neopentylamino)ethyl)-9H-purin-8-yl)methyl)-6- (aziridin- 1 -yl)indolin- 1 -yl)ethanone

lF-10 l-(4-(2-(6-amino-8-((6-(furan-2-yl)indolm^

yl)ethanone

lF-11 4-(2-(8-((6-(lH-pyrazol-3-yl)indolin-5-yl)thio)-6-amino-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

1F-12 4-(2-(6-amino-8-((6-ethynyl-l-methylindolin-5-yl)thio)-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

1F-13 l-(4-(2-(6-arrdno-2-fluoro-8-((6-(furan-2-yl)indolin-5-yl)methyl)-9H-purin-9- yl)ethyl)piperidin-l -yl)ethanone

1F-14 N-(2-((2-(6-amino-8-((6-(furan-2-yl)indolin-5-yl)thio)-9H-purin-9- yl)ethyl)amino)ethyl)sulfamide

1F-15 3-((2-(6-arrdno-8-((6-(furan-2-yl)indolin-5-yl)thio)-9H-pxuin-9-yl)ethyl)amin^

hydroxypropanamide

1F-16 2-chloro-8-((6-iodoindolin-5-yl)methyl)-9-(2-(isobutylarruno)ethyl)-9H-purin-6-amin^

1F-17 9-(3-aminopropyl)-8-((6-iodoindolin-5-yl)thio)-9H-purin-6-amine

1F-18 9-(2-aminoethyl)-8-((6-iodoindolin-5-yl)thio)-9H-purin-6-arnine

1F-19 9-(3-(tert-butylamino)propyl)-8-((6-iodoindolin-5-yl)thio)-9H-purin-6-amine

1F-20 8-((6-iodoindolin-5-yl)thio)-9-(3-(isopropylamino)propyl)-9H-purin-6-amine

Table 1G

1G-6 6-((6-amino-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)thio)indoline-5-carbonitrile

1G-7 8-((5-(furan-2-yl)-l-methylindolin-6-yl)thio)-9-(3-(isopropylamino)propyl)-9H-purin- 6-amine

1G-8 8-((5-cyclobutyl-l-methylindolin-6-yl)methyl)-2-fluoro-9-(2-(isobutylamino)ethyl)- 9H-purin-6 -amine

1G-9 l-(6-((6-amino-2-fluoro-9-(2-(neopen1ylamino)ethyl)-9H-purin-8-yl)methyl)-5- (aziridin- 1 -yl)indolin- 1 -yl)ethanone

lG-10 l-(4-(2-(6-annno-8-((5-(furan-2-yl)m^

yl)ethanone

lG-11 8-(5-(lH-pyrazol-3-yl)indolin-6-ylthio)-9-(2-(l-(methylsulfonyl)piperidin-3-yl)ethyl)

9H-purin-6-amine

1G-12 3-(2-(6-amino-8-(l-ethyl-5-ethynylindolin-6-ylthio)-9H-piu-in-9-yl)ethyl)piperidine carbaldehyde

1G-13 l 3 2 6-amino-2-fluoro-8-((l-methyl-5-(5-methyliuran-2-yl)indolin-6-yl)methyl)- 9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

1G-14 N-(2-((2-(6-amino-8-((5-(fiaran-2-yl)indolin-6-yl)thio)-9H-purin-9- yl)ethyl)amino)ethyl)sulfamide

1G-15 3-(2-(6-amino-8-(5-(5-methylfuran-2-yl)indolm^

N-hydroxypropanamide

1G-16 l-(3-(4-(6-amino-8-(5-iodo-l-methylindolin-6-ylthio)-9H-purin-9-yl)butyl)pyrrolidin- l-yl)ethanone

1G-17 9-(3-aminopropyl)-8-((5-iodoindolin-6-yl)thio)-9H-purin-6-amine

1G-18 9-(2-aminoethyl)-8-((5-iodoindolin-6-yl)thio)-9H-purin-6-amine

1G-19 9-(3-(tert-bu1ylamino)propyl)-8-(l-ethyl-5-iodoindolin-6-ylthio)-9H-purin-6-amine

1G-20 8-(5-iodo-l-methylindolin-6-ylthio)-9-(3-(isopropylamino)propyl)-9H-purin-6-amine

1G-21 l-(3-(6-amino-8-(5-iodo-l-methylindolin-6-ylthio)-9H-piu-in-9-yl)propyl)pyrrolidin-3- one

Table 1H

No. Name

IH-l 6-((6-amino-9-(2-(isobu1ylamino)ethyl)-9H-piu-in-8-yl)thio)-5-iodo-2,3-dihydro-lH- inden-l-one

1H-2 6-((6-amino-9-(3-(isopropylamino)propyl)-9H-pran-8-yl)thio)-5-iodo-2,3-dihydro- lH-inden-l-one

1H-3 6-((6-amino-2-fluoro-9-(2-(isobu1ylamino)ethyl)-9H-purin-8-yl)methyl)-5-iodo-2,3- dihydro- 1 H-inden- 1 -one

1H-4 6-((6-amino-2-fluoro-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)methyl)-5-iodo- 2,3 -dihydro- 1 H-inden- 1 -one

1H-5 6-(6-amino-9-(2-hydroxy-3-(isopropylamino)propyl)-9H-purin-8-ylthio)-5-iodo-2,3- dihydro- 1 H-inden- 1 -one

1H-6 6-((6-amino-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)thio)-5-( iran-2-yl)-2,3- dihydro- 1 H-inden- 1 -one

IH-7 6-((6-amino-2-fluoro-9-(2-(neopen1ylamino)ethyl)-9H-purin-8-yl)methyl)-5- (dimethylamino)-2 ,3 -dihydro- 1 H-inden- 1 -one

1H-8 6-((6-amino-9-(2-(isobu1ylamino)ethyl)-9H-piirin-8-yl)thio)-5-(lH-pyrazol-3-yl)- 2,3-dihydro- 1 H-inden- 1 -one

1H-9 6-((6-amino-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)thio)-5-cyclopropyl-2,3- dihydro- H-inden- 1 -one - lH-10 6-((6-arnino-2-fluoro-9-(2-(isobu1ylarnino)eto^

2 , 3 -dihydro- 1 H-inden- 1 -one

lH-11 6-((6-amino-2-fluoro-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)methyl)- 1 -oxo- 2,3 -dihydro- 1 H-indene-5 -carbonirrile

1H-12 6-((6-amino-2-chloro-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)methyl)-5- (furan-2 -yl)-2,3 -dihydro- 1 H-inden- 1 -one

1H-13 4-(2-(6-amino-8-((6-ethynyl-3-oxo-2,3-dihydro-lH-inden-5-yl)thio)-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

1H-14 6-((9-(2-(l-ace1ylpiperidin-3-yl)ethyl)-6-amino-2-fluoro-9H-purin-8-yl)methyl)-5- (5 -methylfuran-2 -yl)-2,3 -dihydro- 1 H-inden- 1 -one

1H-15 N-(2-((2-(6-armno-8-((6-(furan-2-yl)-3-oxo-2,3-dihydro-lH-inden-5-yl)thio)-9H- purin-9-yl)ethyl)amino)ethyl)sulfarnide

1H-16 3-((2-(6-amino-8-((6-ethynyl-3-oxo-2,3-dihydro-lH-inden-5-yl)thio)-9H-purin-9- yl)ethyl)amino)-N-hydroxypropanamide

1H-17 6-((6-amino-9-(2-(isobutylamino)ethyl)-9H-purin-8-yl)thio)-5-iodo-2,3-dihydro-lH- indene-l-thione

1H-18 6-((6-amino-2-fluoro-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)methyl)-5-iodo- 2,3-dihydro- 1 H-indene- 1 -thione

1H-19 6-((6-amino-9-(3 -aminopropyl)-9H-purin-8 -yl)thio)-5 -iodo-2 ,3 -dihydro- 1 H-inden- 1-one

1H-20 6-((6-amino-9-(2-aminoethyl)-2-fluoro-9H-purin-8-yl)methyl)-5-iodo-2,3-dihydro- 1 H-inden- 1 -one

1H-21 6-((6-amino-9-(2-aminoethyl)-9H-purin-8-yl)thio)-5 -iodo-2,3 -dihydro- 1 H-inden- 1 - one

1H-22 6-((6-amino-9-(3-(tert-butylamino)propyl)-9H-purin-8-yl)thio)-5-iodo-2,3-dihydro- 1 H-inden- 1 -one

1H-23 3-(2-(6-amino-8-(6-iodo-3-oxo-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)ethyl)pyrrolidine- 1 -carbaldehyde

1H-24 6-(6-amino-9-(2-(l-(methylsulfonyl)pyrrolidin-3-yl)ethyl)-9H-purin-8-ylthio)-5- iodo-2,3 -dihydro- 1 H-inden- 1 -one

1H-25 N-(3-(6-amino-2-fluoro-8-((6-iodo-3-oxo-2,3-dihydro-lH-inden-5-yl)methyl)-9H- purin-9-yl)propyl)methanesulfonamide

1H-26 6-((6-amino-9-(2-(l-(methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-8-yl)thio)-5- ( 1 H-pyrazol -3 -yl)-2 ,3 -dihydro- 1 H-inden- 1 -one

1H-27 6-((6-amino-9-(3-(tert-bu1ylamino)propyl)-9H-purin-8-yl)thio)-5-(5-methylfuran-2- yl)-2,3 -dihydro- 1 H-inden- 1 -one

1H-28 6-((6-amino-9-(3-(tert-bu1ylamino)propyl)-9H-purin-8-yl)thio)-5-(5-methylthiazol- 2-yl)-2,3-dihydro-lH-inden-l -one

1H-29 6-((6-amino-9-(3-(tert-bu1ylamino)propyl)-9H-purin-8-yl)thio)-5-(thiophen-2-yl)- 2,3-dihydro-lH-inden- 1 -one

1H-30 2-(3-(6-amino-8-(6-iodo-3-oxo-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)propyl)azetidine- 1 -carbaldehyde

1H-31 6-((6-amino-9-(3-(tert-butylamino)propyl)-2-fluoro-9H-purin-8-yl)methyl)-5- ethynyl-2,3 -dihydro- 1 H-inden- 1 -one

1H-32 6-((6-amino-9-(2-(l-(methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-8-yl)thio)-5- (dimethylamino)-2,3-dihydro-lH-inden-l -one

1H-33 6-((6-amino-9-(3-(tert-bu1ylarnino)propyl)-2-fluoro-9H-purin-8-yl)methyl)-5-(5- methyloxazol-2-yl)-2,3-dihydro-lH-inden-l-one

1H-34 6-((9-(2-(l-ace1ylpiperidin-4-yl)ethyl)-6-amino-2-fluoro-9H-purin-8-yl)rnethyl)-5- (5-methylthiazol-2-yl)-2,3-dihydro-lH-inden-l-one

H-35 N-(3-(6-amino-2-fluoro-8-((6-(5-methyloxazol-2-yl)-3-oxo-2,3-dihydro-lH-inden- 5-yl)methyl)-9H-purin-9-yl)propyl)methanesulfonamide

H-36 l-(3-(6-amino-8-(6-iodo-3-oxo-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)propyl)pyrrolidin-3 -one

H-37 l-(3-(6-amino-8-(6-iodo-3-oxo-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9- yl)propyl)pyrrolidin-2-one

Table 2A

Table 2B

Table 2C

Table 2D

Table 2E

Table 2G

Table 2H

Table 2I

Table 2J

Table 2K

Table 2L

Table 2N

Table 20

Table 2P

Table 2Q

Table 3A

No. Name

3A-1 2-fluoro-8-((5-(furan-2-yl)benzofuran-6-yl)methyl)-9-(2- (neopentylamino)ethyl)-9H-purin-6 -amine

3A-2 2-fluoro-8-((5 -iodo- 1 H-indol-6-yl)methyl)-9-(2-(neopentylamino)ethyl)- 9H-purin-6-amine

3A-3 N-(2-((2-(6-amino-8-((5-(furan-2-yl)benzo[b]thiophen-6-yl)thio)-9H- purin-9-yl)ethyl)amino)ethyl)sulfamide

3A-4 3-((2-(8-((l-acetyl-5-(furan-2-yl)-lH-indol-6-yl)thio)-6-amino-9H- purin-9-yl)ethyl)amino)-N-hydroxypropanamide

3A-5 8-((5-(azetidin-l-yl)benzof ran-6-yl)thio)-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine

3A-6 8-((5-iodobenzo[b]thiophen-6-yl)thio)-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine

3A-7 8-((5-(lH-pyrazol-3-yl)-lH-indol-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

3A-8 8-((5-ethynyl-lH-indol-6-yl)thio)-9-(2-(isobutylamino)ethyl)-9H-purin- 6-amine

3A-9 6-((6-amino-9-(2-(isobutylamino)ethyl)-9H-purin-8-yl)thio)-l-methyl- 1 H-indole-5 -carbonitrile

3A-10 9-(3-aminopropyl)-8-((5-iodobenzofuran-6-yl)thio)-9H-purin-6-amine

3A-11 9-(2-aminoethyl)-8-((5-iodobenzofuran-6-yl)thio)-9H-purin-6-amine

3A-12 9-(3-(tert-butylamino)propyl)-8-((5-iodobenzofuran-6-yl)thio)-9H-purin- 6-amine

3A-13 2-fluoro-8 -((5 -iodobenzofuran-6 -yl)methyl)-9-(2 - (neopentylamino)ethyl)-9H-purin-6-amine

3A-14 l-(4-(2-(6-amino-2-fluoro-8-((5-(5-methylfuran-2-yl)benzof ran-6- yl)methyl)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

3A-15 9-(3-(tert-butylamino)propyl)-8-((5-ethynylbenzofuran-6-yl)thio)-9H- purin-6-amine

3A-16 2-fluoro-8-((5-(5-methyloxazol-2-yl)benzof ran-6-yl)methyl)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

3A-17 9-(3-(tert-butylamino)propyl)-8-((5-(dimethylamino)benzofuran-6- yl)thio)-9H-purin-6-amine

3A-18 8-((5-iodobenzofuran-6-yl)thio)-9-(2-(l-(methylsulfonyl)piperidin-3- yl)ethyl)-9H-pnrin-6-amine

3A-19 8-((5 -( 1 H-pyrazol-3 -yl)benzofuran-6-yl)thio)-9-(2 - (neopentylamino)ethyl)-9H-purin-6-amine

3A-20 N-(3 -(6 -amino-8 -((5 -(aziridin- 1 -yl)benzofuran-6-yl)thio)-9H-purin-9- yl)propyl)methanesulfonamide

3A-21 3 -(6 -amino-8 -((5 -iodobenzofuran-6 -yl)thio)-9H-purin-9-yl)propyl

sulfamate

3A-22 9-(3-(tert-butylamino)propyl)-8-((5-(oxazol-2-yl)benzofuran-6-yl)thio)- 9H-purin-6-amine _

3A-23 8-((5-ethynylbenzofuran-6-yl)thio)-9-(2-(neopentylamino)ethyl)-9H- purin-6-amine

3A-24 l-(6-amino-8-((5-iodobenzofuran-6-yl)thio)-9H-purin-9-yl)-3-(tert- butylamino)propan-2-ol

3A-25 8-((5-iodobenzofuran-6-yl)thio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine

3A-26 9-(3-(tert-butylamino)propyl)-8-((5-(5-methylthiazol-2-yl)benzofuran-6- yl)thio)-9H-purin-6-amine

Table 3B

No. Name

3B-1 5- ((6-amino-9-(2-(isobutylamino)ethyl)-9H-purin-8-yl)thio)benzofuran-

6- carbonitrile

3B-2 8-((6-iodobenzofiiran-5-yl)thio)-9-(2-(isobutylamino)ethyl)-9H-purin-6- amine

3B-3 8-((6-(furan-2-yl)benzofuran-5-yl)thio)-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine

3B-4 N-(2-((2-(6-amino-8-((6-(furan-2-yl)benzofuran-5-yl)thio)-9H-purin-9- yl)ethyl)amino)teeth)sulfamide

3B-5 3-((2-(6-amino-8-((6-(furan-2-yl)benzofuran-5-yl)thio)-9H-purin-9- yl)ethyl)amino)-N-hydroxypropanamide

3B-6 2-fluoro-8-((6-(iuran-2-yl)-lH-indol-5-yl)methyl)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine __

3B-7 8-((6-(azetidin-l-yl)-lH-indol-5-yl)thio)-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine

3B-8 9-(2-(isobutylamino)ethyl)-8-((6-(pyrrolidin-l-yl)-lH-indol-5-yl)thio)- 9H-purin-6-amine

3B-9 8-((6-(furan-2 -yl) - 1 -methyl- 1 H-indol-5 -yl)thio)-9-(3 - (isopropylamino)propyl)-9H-purin-6-amine

3B-10 2-fluoro-8-((6-iodo-l-isopropyl-lH-indol-5-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

3B-11 2-fluoro-8-((6-(furan-2-yl)benzo[b]thiophen-5-yl)methyl)-9-(3- (isopropylamino)propyl)-9H-purin-6-ainine

3B-12 2-fluoro-8-((6-iodobenzo[b]thiophen-5-yl)methyl)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

3B-13 8-((6-(lH-pyrrol-3-yl)benzo[b]thiophen-5-yl)methyl)-2-fluoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

3B-14 8-((6-ethynylbenzo[b]thiophen-5-yl)methyl)-2-fluoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

3B-15 2-chloro-8-((6-ethynylbenzo[b]thiophen-5-yl)methyl)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

3B-16 8-((6-(azetidin-l-yl)-lH-indol-5-yl)thio)-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine

3B-17 8-((6-(aziridin -yl) H-indol-5-yl)thio)-9-(2-(isobutylainino)ethyl)-9H- purin-6-amine

3B-18 9-(3-aminopropyl)-8-((6-iodobenzofuran-5-yl)thio)-9H-purin-6-amine

3B-19 9-(2-aminoethyl)-8-((6-iodobenzofuran-5-yl)thio)-9H-purin-6-amine

3B-20 9-(3-(teit-butylamino)propyl)-8-((6-iodobenzofuran-5-yl)thio)-9H- purin-6-amine

3B-21 l-(4-(2-(6-amino-2-fluoro-8-((6-(5-methylfuran-2-yl)benzofuran-5- yl)methyl)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

3B-22 9-(3-(tert-butylamino)propyl)-8-((6-ethynylbenzofuran-5-yl)thio)-9H- purin-6-amine

3B-23 2-fluoro-8-((6-(5-methyloxazol-2-yl)benzofuran-5-yl)methyl)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

3B-24 9-(3-(tert-bu1ylamino)propyl)-8-((6-(dimethylaniino)benzofuran-5- yl)thio)-9H-purin-6-amine

3B-25 8-((6-iodobenzofuran-5-yl)thio)-9-(2-(l-(methylsulfonyl)piperidin-3- yl)ethyl)-9H-purin-6-amine

3B-26 8-((6-(lH-pyrazol-3-yl)benzof ran-5-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

3B-27 N-(3-(6-annno-8-((6-(aziridin-l-yl)benzofuran-5-yl)thio)-9H-pxirin-9- yl)propyl)methanesulfonamide

3B-28 3-(6-amino-8-((6-iodobenzofuran-5-yl)thio)-9H-pxirin-9-yl)propyl

sulfamate

3B-29 9-(3-(tert-butylamino)propyl)-8-((6-(oxazol-2-yl)benzofuran-5-yl)thio)- 9H-purin-6-amine

3B-30 8-((6-ethynylbeiizofuran-5-yl)thio)-9-(2-(neopentylaniino)ethyl)-9H- purin-6-amine

3B-31 l-(6-annno-8-((6-iodobeiizofuran-5-yl)thio)-9H-piirin-9-yl)-3-(tert- butylamino)propan-2-ol

3B-32 8-((6-iodobenzofuran-5-yl)thio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine

3B-33 9-(3-(tert-bu1ylaniino)propyl)-8-((6-(5-methylthiazol-2-yl)beiizofuran-5- yl)thio)-9H-purin-6-amine

3B-34 l-(3-(6-amino-8-(6-iodobenzofuran-5-ylthio)-9H-pxirin-9- yl)propyl)pyrrolidin-3 -ol

3B-35 l-(3-(6-amino-8-(6-iodobenzofuran-5-ylthio)-9H-pitrin-9- yl)propyl)pyrrolidin-3 -one

Table 3C

No. Name

3C-1 6-((6-amino-9-(2-(isobutylamino)ethyl)-9H-purin-8-yl)thio)benzo[d]oxazole-5- carbonitrile

3C-2 8-((5-(furan-2-yl)benzo[d]thiazol-6-yl)thio)-9-(2-(neopen1ylamino)ethyl)-9H- purin-6-amine

3C-3 2-fluoro-8-((5-(furan-2-yl)benzo[d]oxazol-6-yl)methyl)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

3C-4 8-((5-(azetidin-l -yl)benzo[d]oxazol-6-yl)thio)-9-(2-(isobu1ylamino)ethyl)-9H- purin-6-amine

3C-5 9-(2-(isobu1ylannno)ethyl)-8-((5-(pyrrolidin-l-yl)benzo[d]thiazol-6-yl)thio)-9H- purin-6-amine

3C-6 8-((5-ethynylberizo[d]thiazol-6-yl)methyl)-2-fluoro-9-(2-(isobutylamino)ethyl)- 9H-purin-6-amine

3C-7 N-(2-((2-(6-amino-8-((5-iodobenzo[d]oxazol-6-yl)thio)-9H-purin-9- yl)ethyl)amino)ethyl) sulfamide

3C-8 3 -((2-(6-amino-8 -((5 -(furan-2-yl)benzo [d] oxazol-6 -yl)thio)-9H-pxirin-9- yl)ethyl)amino)-N-hydroxypropanamide No, Name

3D-1 5-((6-amino-9-(2-(isobu1ylamino)ethyl)-9H-purin-8-yl)thio)-lH- benzo[d]imidazole-6-carbonitrile

3D-2 8-((6-(furan-2-yl) H-benzo[d]imidazol-5-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

3D-3 2-fluoro-8-((6-(furan-2-yl)-lH-benzo[d]imidazol-5-yl)rnethyl)-9-(2- (neopentylamino)ethyl)-9H-purin-6-aniine

3D-4 8-((6-(azetidin- l-yl)-l H-benzo[d] imidazol-5 -yl)thio)-9-(2- (isobutylamino)ethyl)-9H-purin-6-arnine

3D-5 9-(2-(isobutylaniino)ethyl)-8-((6-(pyrroHdin-l -yl)-lH-benzo[d]imidazol-5- yl)thio)-9H-purin-6-amine

3D-6 8-((6-ethynyl-l-methyI-lH-benzo[d]imidazol-5-yl)methyl)-2-fluoro-9-(2-

(isobutylamino)ethyl)-9H-purin-6-amine

3D-7 N-(2-((2-(8-((l-acetyl-6-iodo-lH-benzo[d]imidazol-5-yl)thio)-6-amino-

9H-purin-9-yl)ethyl)amino)ethyi)methanesulfonam.ide

3D-8 3-((2-(6-amino-8-((6-(furan-2-yl) H^

piirin-9-yl)ethyl)amino)-N-hydroxypropanamide

3D-9 5-((6-amino-9-(2-(isobutylamino)ethyl)-9H-purin-8- y!)thio)benzo [d] oxazole-6-carbonitrile

3D-10 8-((6-iodobenzo[d]oxazol-5-yl)thio)-9-(2-(neopentylamino)ethyl)-9H- purin-6-amine

3D-11 5-((6-amino-9-(2-(isobutylamino)ethyl)-9H-purin-8- yl)thio)benzo[d]thiazole-6-carbonitrile

3D-12 8 -((6 -(furan-2 -y l)benzo [d 1 thiazol-5 -yl)thio)-9 -(2-(neopentylamino)ethyl)- 9H-purin-6-amine

3B-13 2-fluoro-8-((6-(furan-2-yl)benzo[d]oxazol-5-yl)methyl)-9-(2-

(neopentylamino)ethyl)-9H-purin-6-aiTiine

3D-14 8-((6-(azetidin-l-yl)benzo[d]oxazol-5-yl)thio)-9-(2-(isobutylamino)ethyl)-

9H-purin-6-amine

3D-15 9-(2-(isobuiylamino)ethyl)-8-((6-(pyrrolidin-l-yl)benzo[d]thiazol-5- yl)thio)-9H-purin-6-amine

3D-16 8-((6-ethynylbenzo[d]thiazol-5-yl)methyl)-2-fluoro-9-(2-

(isobutylamino)ethyl)-9H-purin-6-amine

Table 3E

No. Name

3E-1 6-((6-amino-9-(2-(isobutylamino)ethyl)-9H-purin-8-yl)thio)benzo[d][l,2,3]oxadiazole- carbonitrile

3E-2 8-((5-(iiiran-2-yl)beRzo[d][l,2,3]tM^

6-amine

3E-3 2-fluoro-8-((5-(furan-2-yl)benzo[d][l ,2,3]oxadiazol-6-yl)methyl)-9-(2- (neopentylarnino)ethyl)-9H-piirin-6-amine

3E-4 8-((5-(azetidin-l -yl)benzo[d] [ 1 ,2,3]oxadiazol-6-yl)thio)-9-(2-(isobutylamino)ethyl)-9H- purm-6-amine

3E-5 9-(2-(isobutylarnmo)ethyl)-8-((5-(pyrrolidm-l -yl)benzo[d] [ 1 ,2,3]thiadiazol-6-yl)thio)-9H-

- - -azet n- -y - -n azo- -yt o- - - so u1yamnoet y - -pu

Table 3F

Tab!e 4A

No, Name

4A-1 DZ2-388

9-(3-(isopropylamino)propyl)-8-(6-phenylbenzo[d][l,3]dioxol-5-ylthio)-

9H-purin-6-amine

4A-2 DZ2-390

8-(6-(4-tert-butylphenyl)benzo[d][l,3]dioxol-5-ylthio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

4A-3 DZ2-391

8-(6-(3,5-bis(trifluoromethyl)phenyl)benzo[d][l,3]dioxol-5-ylthio)-9-(3- (isopropylamino)propyl)-9H-purin-6-ainine

4A-4 TT-V-47B

Nl-(3-(6-amino-8-(6-(3,5-bis(trifluoromethyl)phenyl)benzo[d][l,3]dioxol- 5-ylthio)-9H-purin-9-yl)propyl)hexane-l,6-diamine

4A-5 DZ2-392

8-(6-(4-(dimethylamino)phenyl)benzo[d][l,3]dioxol-5-ylthio)-9-(3-

(isopropylamino)propyl)-9H-purin-6-amine

4A-6 DZ3-3

9-(3-(isopropylamino)propyl)-8-(6-(4-methoxyphenyl)benzo[d][l,3]dioxol- 5-ylthio)-9H-purin-6-amine

4A-7 DZ3-6

8-(6-(4-bromophenyl)b.enzo[d] [ 1 ,3]dioxol-5-ylt io)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

4A-8 DZ3-50

4-(6-(6-amino-9-(3-(isopropyIamino)propyl)-9H-purin-8- ylthio)benzo[d][ 1 ,3]dioxol-5-yl)benzaldehyde

4A-9 4-(2-(6-amino-8-(6-phenylbenzo[d][l ,3]dioxol-5-ylthio)-9H-purin-9- __ yl)ethyl)piperidine-l-carbaldehyde

l-(4 (2-(6-amino-2-fluoro-8-((6-phenylbenzo[d][l,3]dioxol-5-yl)methyl)- 9H-purin-9-yl)ethyl)piperidin-l-yl)ethanone

- 4A-11 N-(2-((2-(6-amino-8-((6-phenylbenzo[d] [ 1 ,3]dioxol-5-yl)thio)-9H-purin-9- yl)ethyl)amino)ethyl)sulfamide

4A-12 3-(2-(6-amino-8-(6-phenylbenzo[d][l,3]dioxol-5-ylthio)-9H-purin-9- yl)ethy]amino)-N-hydroxypropanamide

4A-13 9-(3-aminopropyl)-8-(6-phenylbenzo[d][l,3]<lioxol-5-ylthio)-9H-purin-6- amine

"able 4B

No. Name

4B-1 PU-WS8

8-(6-ethynylbenzo[d]fl,3]dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-

9H-puxin-6-amine

4B-2 PU-WS6

8-(6-(3_J3-dimethylbut-l-ynyl)benzo[d][l,3]dioxol-5-ylthio)-9-(3-

(isopropylamino)propyl)-9H-purin-6-amine

4B-3 PU-WS7

9-(3-(isopropylamino)propyl)-8-(6-(phenylethynyl)benzo[d][l,3]dioxol-5- ylthio)-9H-purin-6-amine B-4 PU-WS16

8-(6-elJiynylbenzo[d][l,3]dioxol-5-ylthio)-9-(2-(isobutylaniino)ethyl)-9H- purin-6-amine

B-5 l-(3-(2-(6-anuno-8-(6-etihynylbenzo[d][l,3]dioxol-5-ylthio)-9H-purin-9-- yl)ethyl)piperidin- 1 -yl)ethanone

B-6 8-(6-ethynylbenzo[d][l,3]dioxol-5-ylthio)-9-(2-(l- (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine

B-7 l-(3-(4-(6-amino-8-((6-ethynylbenzo[d][l,3]dioxol-5-yl)methyl)-2-fluoro- 9H-purin-9-yl)butyl)pyrrolidin-l-yl)ethanone

B-8 5-(6-amino-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9H- purin-9-yl)pentane- 1 -sulfonamide

B-9 3-(2-(6-araino-2-chloro-8-((6-ethynylbenzo[d][l,3]dioxol-5-yl)methyl)- 9H-purin-9-yl)ethyl)piperidine- 1 -carbaldehyde

B-10 3-(2-(6-amino-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro- 9H-purin-9-yl)ethyl)piperidine-l -sulfonamide

B-11 N-(2-((2-(6-amino-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)thio)-9H-purin- 9-yl)ethyl)amino)et yl)sulfamide B-12 3-(2-(6-annno-8-(6-ethynylbenzo[d][l,3]dioxol-5-ylthio)-9H-purin-9- yl)ethylamino)-N-hydroxypropanamide

B-13 PU-WS1

8-(6-ethynylbenzo[d][l,3]ch^xol-5-ylthio)-9-(2-(neopen1ylamino)ethyl)-

9H-purin-6-amine

B-14 PU-WS20

8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

B-15 9-(3 -aminopropyl)-8-(6-ethynylbenzo[d] [ 1 ,3] dioxol-5 -ylthio)-9H-purin-6- amine

B-16 9-(2-aminoethyl)-8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-6- amine

B-17 9-(3-(tert-butylamino)propyl)-8-(6-ethynylbenzo[d][l,3]dioxol-5-ylthio)-

9H-purin-6-amine

B-18 1 -(3-(6-amino-8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-9- y l)propyl)pyrroli din-3 -one

B-19 3-(2-(6-aniino-8-((6-ethynyIbenzo[d][l,3]cHoxol-5-yl)thio)-9H-pui-in-9- yl)ethyl)piperidine-l -sulfonamide

B-20 6-(6-amino-8-(6-ethynylbenzo[d][l,3]dioxol-5-ylthio)-9H-purin-9- yl)hexanamide

B-21 l-(6-amino-8-(6-ethynylbenzo[d][l,3]dioxol-5-ylthio)-9H-purin-9-yl)-3- (tert-butylamino)propan-2-ol

B-22 6-(6-amino-8-((6-ethynylbenzo[d][l,3]dioxol-5-yl)methyl)-2-fluoro-9H- purin-9-yl)hexanamide

B-23 l-(2-((2-(6-amino-8-(6-ethynylbenzo[d][l,3]dioxol-5-ylthio)-9H-purin-9-^' yl)ethylamino)methyl)pyrrolidin- 1 -yl)ethanone

B-24 5-(6-amino-8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-9- yl)pentane- 1 -sulfonamide

B-25 l-(3-(2-(6-amino-8-((6-ethynylbenzo[d][l,3]dioxol-5-yl)methyl)-2-fluoro- 9H-purin-9 -yl)ethyl)p iperidin- 1 -yl)ethanone

B-26 8-((6-ethynylbenzo[dj [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9-(2- (neopentylarnino)ethyl)-9H-purin-6-amine

4B-27 8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

4B-28 9-(3-(tert-butylamino)propyl)-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5- yl)methy])-2-fluoro-9H-purin-6-amine

4B-29 8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9-(2-( 1 - (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine

4B-30 I -(3-(6-amino-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro- 9H-purin-9 -yl)propyl)pyrrolidin-3 -one

4B-31 8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9-(2-( 1 - methylpiperidin-3-yl)ethyl)-9H-piirin-6 -amine

4B-32 8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9-(2-( 1 -methylpiperidin-2- yl)ethyl)-9H-purin-6-amine

4B-33 l-(2-((2-(6-amino-8-((6-ethynylbenzo[d][l,3]dioxol-5-yl)methyl)-2- fluc^yro-9H-purin-9-yl)ethylaniino)memyl)pyrrolidm-l-yl)ethanone

4B-34 8-(6-ethynylbenzo[d][l ,3]dioxol-5-ylthio)-9-(2-(l-methylpiperidin-3- yl)ethyl)-9H-purin-6 -amine

4B-35 8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9-(2-( 1 - methylpiperidin-2-yl)ethyl)-9H-purin-6-amine

Table 4C

No. Name

4C-1 DZ3-4

8-((6-(furan-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine

4C-2 DZ3-27

8-((6-(mran-3-yl)benzo[d][l,3]dioxol-5-yl)mio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine

4C-3 DZ3-25

2-fluoro-8-((6-(mran-2-yl)benzo[d][l,3]dioxol-5-yl)methyl)-9-(2-(isobu1ylamino)ethyl)- 9H-purin-6-amine

4C-4 DZ3-26

2-fluoro-8 -((6-(furan-3 -yl)benzo [d] [ 1 ,3 ] dioxoi-5 -yl)methyl)-9 -(2-(isobutylamino)et yl)-

9H-purin-6-amme

4C-5 TT5-53A

8-((6-(fiiran-2-yl)benzo[d][l,3]dioxol-5-y

6-amine

4C-6 DZ3-33

5-(6-((6-aniino-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)thio)benzo[d][l,3]dioxol-5- yl)furan-2-carbaldehyde

4C-7 DZ3-34

5-(6-((6-amino-2-fluoro-9-(2-(isobut amino)ethyl)-9H-purin-8- yl)methyl)benzo[d][l,3]dioxol-5-yl)furan-2-carbaldehyde

4C-8 DZ3-35

9-(3 -(i sopropylamino)propyl)-8 -((6 -(5 -methylfuran-2 -yi)benzo [d] [ 1 ,3] dioxol-5 -yl)thio)-

9H-purin-6-amine

4C-9 DZ3-36

2-fluoro-9-(2-(isobu1ylamino)emyl)-8-((6-(5-memylfuran-2-yl)benzo[d][l,3]dioxol-5- yl)methyl)-9H-purin-6-amine

C-1Q DZ3-49

9-(3-(isopropylamino)propyl)-8-((6-(isoxa2oM-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H- purin-6-amine

C-11 DZ3-51

2-fluoro-9-(2-(isobu1ylamino)ethyl)-8-((6-(isoxazol-4-yl)benzo[d][l,3]dioxol-5- yl)methyl)-9H-purin-6-amine

C-12 8-((6-(5-(aminomethyl)furan-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-aniine

C-13 8-((6-(5-(annnome l)furan-2-yl)be^^

(isobutylaniino)ethyl)-9H-purin-6-amme

C-14 DZ3-60

8-((6-(mran-3-yl)ben2o[d][1 ]dioxol-5-yl)ihio)-9-(2-(neopeiitylainIn

6-amine

C-15 8-(6-(5-methyloxazol-2-yl)benzo[d][I,3](Hoxol-5-ylthio)-9-(2-(neopen1ylamino)ethyl)- 9H-purin-6-amine

C-16 DZ3-56

8-((6-(5-m.ethyliuran-2-y!)benzo[^^

9H-purin-6-amine

C-17 l-(3-(6-amino-2-fluoro-8-((6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5-yl)methyl)- 9H-purin-9-yl)propyl)pyrrolidin-3-one

C-18 8-((6-(5-(aminomethyl)furan-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

C-19 l-(3-(2-(6-ainino-8-(0-(5-met.M

yl)ethyl)piperidin- 1 -yl)ethanone

C-20 8 6 5-methylfliran-2-yl)benzo[d][l ,3]dioxoj-5-ylthio)-9-(2-(l- (inethylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6~amine

C-21 1 -(3-(2-(6-amino-2-fluoro-8-((6-(5-methylfuran-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)- 9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

C-22 4-(2-(6-ainino-2-fluoro-8-((6-(fiu-an-2-yl)benzo[d][l,3]dioxol-5-yl)melhyl)-9H-^

yl)ethyl)piperidine- 1 -carbaldehyde

C-23 l-(3-(6-amino-8-(6-(oxazol-2-yl)benzo[d][l,3]dioxol-5-ylthio)-9H-purin-9- yl)propyl)pyrrolidin-3 -one

C-24 6-(6-amino-8-(6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5-ylthio)-9H-purin-9- yl)hexanamide

C-25 6-(6-aniino-2-fluoro-8-((6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5-yl)methyl)-9H- purin-9-yl)liexanamide

C-26 I-(4-(2-(0-aniino-2-fluoro-8-((0-(5-m

9H-purin-9-yl)ethyl)piperidin-l-yl)ethanone

C-27 1 -(4-(2-(6-amino-2-chloro-8-((6-(5-methylfuxan-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)- 9H-purin-9-yl)ethyl)piperidin-l -yl)ethanone

C-28 l-(3-(2-(6-amino-8-(6-(5-me±yloxazol-2-yl)benzo[d][l,3]dioxol-5-ylthio)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone

C-29 l-(3-(2-(6-amino-2-fluoro-8-((6-(5-methyloxazol-2-yI)benzo[d][l,3]dioxol-5- yl)methyl)-9H-purin-9-yl)ethyl)piperidin-l-yl)ethanone

C-30 3-(2-(6-ainino-2-chloro-8-((6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5-yl)methyl)- 9H-purin-9-yl)ethyl)piperidine- 1 -sulfonamide

C-31 1 -(4-(2-(6-amino-8-((6-(5-(aminomethyl)furan-2-yl)ben2o[d] [ 1 ,3]dioxol-5-yl)thio)-9H- purin-9-y i)ethyi)piperidin- 1 -yl)ethanone

C-32 l-(3-(2-(6-amino-8-((6-(5-(aniinomethyl)oxazol-2-yl)benzo[d][l,3]dioxol-5-yl)methyl)-

9-(3-(tert-butylamino)propyl)-8-(6-(5-methylfuran-2- yl)benzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-piirin-6-amine

9-(3-(tert-butylamino)propyl)-8-((6-(oxazol-2- yl)berso[d][l,3]dioxol-5-yl)thio)-9H-purin-6-amine

l-(4-(2-(6-amino-8-((6-(oxazol-2-yl)benzo[d][l,3]dioxol-5- yl)thio)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

8-((6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9-(2- (l-(methylsulfonyl)piperidin-3-yl)ethyl)-9H-piirin-6-amine

9-(3-(tert-butylamino)propyl)-8-((6-(5-methyloxazol-2- yl)benzo[d][l,3]dioxol-5-yl)thio)-9H-purin-6-amine

1 -(6-amino-8-(6-(5-methylfuran-2-yl)benzo[d] [ 1 ,3]dioxol-5- ylthio)-9H-purin-9-yl)-3-(tert-buiylamino)propan-2-ol

l-(6-amino-8-((6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5- yl)thio)-9H-purin-9-yl)-3-(isopropylamino)propan-2-ol

2~(3-(6-amino-8-(6-(5-methyif rari"2-yl)beiizo[d][l₅3]dioxol-5- ylthio)-9H-purin-9-yl)propy].)azIridine-l-carbaldehyde

5-(6-amino-8-(6-(5-methylfuran-2-yl)benzo[d][l,3]dioxol-5- ylthio)-9H-purin-9-yl)pentane- 1 -sulfonamide

5-(6-araino-8-(6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5- ylthio)-9H-purin-9-yl)pentane-l-sulfonamide 8-(6-(5-methyloxazol-2-yl)benzo[d][I ,3]dioxol-5-ylihio)-9-(3~

(l-(methylsulfonyl)pyrrolidin-3-yl)propyl)-9H-piuin-6-amine

l-(3-(6-amino-8-(6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol- 5 -ylthi o)-9H-purin -9 -yl)propyl)pyrrolidin-3 -one

9-(3-(tert-butylamino)propyl)-2-fluoro-8-((6-(oxazol-2- yl)benzo[d] [ 1 ,3]dioxoj.-5-yl)methyl)-9H-purin-6-amine

5-(6-amino-2-fluoro-8-((6-(5-methylfuran-2- yl)benzo[d][l,3]dioxol-5-yl)methyl)-9H-purin-9-yl)pentane-l- sulfonamide

6-(6-amino-2-fluoro-8-((6-(5-methylf-iran-2- yl)benzo[d][l,3]dioxol-5-yl)methyl)-9H-pxirin-9-yl)hexanamide

2-fluoro-9-(3-(isopropylamino)propyl)-8-((6-(5-methylfiiran-2- yl)benzo[d][l,3](Uoxol-5-yl)methyl)-9H-purin-6-aniine

9-(3-(tert-butylamino)propyl)-2-fluoro-8-((6-(5-methylfuran-2- yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-p rin-6-amine

■9-(3-aminopropyl)-2-fluoro-8-((6-(5-methylfuran-2- yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-pimn-6-amine

9-(3-aminopropyl)-2-fluoro-8-((6-(5-methyloxazol-2- yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-purin-6-amine

Table 4D No. Name

D-1 DZ2-395

9-(3-(isopropylaniino)propyl)-8-((6-(thiophen-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H- purin-0-arnine

D-2 DZ3-48

2-fluoro-9-(2-(isobu1ylamino)ethyl)-8-((6-(1liiophen-2-yl)beiizo[d][l,3]dioxol-5- yl)methyl)-9H-purin-6-amine

D-3 DZ3-58

9-(2-(neopentylanuno)ethyl)-8-((6-(thiophen-2-yl)benzo[d][l,3]ch^xol-5-yl)thio)-9H- purin-6-amine

D-4 9-(3 -(isopropylamino)propyl)-8-((6-(thiophen-3 -yl)benzo [d] [ 1 ,3] dioxol-5 -yl)thio)-9H- purin-6-amine

D-5 2-fluoro-9-(2-(isobutylamino)ethyl)-8-((6-(thiophen-3-yl)benzo[d][l,3]dioxol-5- yl)methyl)-9H-purin-6-amine

D-6 9-(2-(neopentylamino)ethyl)-8-((6-(thiophen-3-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H- purin-6-amine

D-7 l-(4-(2-(6-amino-8-((6-(1±iiophen-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)etharione

D-8 4-(2-(6-amino-8-((6-(thiophen-2-yl)benzo[d][l ,3]dioxol-5-yl)thio)-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

D-9 l-(4-(2-(6-amino-2-:¾oro-8-((6-^

purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

D-10 4-(2-(6-amino-2-fluoro-8-((6-(thiophen-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H- purin-9-yl)ethyl)piperidine-l -carbaldehyde

D-11 4-(2-(6-amino-8-((6-(thiophen-3-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

D-12 4-(2-(6-amino-2-fluoro-8-((6-(thiophen-3-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H- purin-9-yl)ethyl)piperidine- 1 -carbaldehyde

D-13 4-(2-(6-amino-2-chloro-8-((6-(thiophen-3-yl)benzo[d][l,3]dioxol-5-yl)methyl)-9H- purin-9-yl)ethyl)piperidine- 1 -carbaldeh de

D-14 N-(2-((2-(6-anuno-8-((6-(1¾iophen-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H-purin-9- yl)ethyl)amino)ethyl) sulfamide

D-15 3-((2-(6-amino-8-((6-(thiophen-2-yl)ben2o[d][l,3]dioxol-5-yl)thio)-9H-purin-9- yl)ethyl)amino)-N-hydroxypropanarnide

D-16 DZ4-21

9-(3-(isopropylamino)propyl)-8-((6-(thiazol-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H- purin-6-amine

D-17 DZ4-24

2-fluoro-9-(2-(isobutylamino)ethyl)-8-((6-(thiazol-2-yl)benzo[d][l,3]dioxol-5- yl)methyl)-9H-purin-6-amine

D-18 9-(3-arninopropyl)-8-((6-(1hiophen-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H-purin-6- amine

D-19 9-(3-(tert-butylamino)propyl)-8-((6-(thiophen-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9H- purin-6-amine

D-20 9-(3-(tert-butylamino)propyl)-8-((6-(thiazol-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9H- purin-6-amine

D-21 9-(3 -(tert-butylamino)propyl)-8 -((6-(5-methy lthiophen-2 -yl)benzo [d] [ 1 ,3] dioxol-5 - yl)thio)-9H-purin-6-amine

D-22 9-(3-(tert-butylanxino)propyl)-8-((6-(5-meth^

9H-purin-6-amine

Table 4E

No. Name

4E-1 PU- S3

6-((6-anuno-2-fluoro-9-(3-(isopropylamino)propyl)-9H-purin-8- yi)methyl)benzord] [1 ,3] dioxoie-5 -carbonitrile

4E-2 PU-WSS

6-((6-amino-9-(3-(isopropyIamino)propyl)-9H-purin-8-yl)thio)benzo[d][l,3]dioxole-5- carbonitrile

4E-3 DZ3-39

2-(6-((6-ammo-9~(3-(isopropylammo)p^

yl)acetonitrile

4E-4 DZ3-40

2-(6~((6-amino-2-fluoro-9-(2-(isobuty^rlamino)ethyl)~9H-pttrin"8- yl)methyl)benzo[d^"i[l,3]dioxol-5-yl)acetonitrile

6E~5 N-(2-((2-(6-amino-8-((6-(cyanomethyl)benzo[d][l,3]dioxol-5-yl)thio)-9H-p rin-9- yl)ethyl)amino)et yl)sulfamide

4E-6 3-((2-(6-amino-8-((6-cyanobenzo[d][l,3]dioxol-5-yl)thio)-9H-piirin-9-yl)eih^

hydroxYpropanami.de

4E-7 6-((6-amino-2-chloro-9-(3-(isopropylamino)propyl)-9H-purin-8- yl)methyI)beri2:o[d^"iri,3]dioxo3e-5-carborjitriie

Table 4F

No. Name

4F-1 DZ3-5 9-(3-(isopropylamino)propyl)-8-(6-^yridh -4-yl)benzo[d][l,3]dioxol-5- ylthio)-9H-purin-6-amine

Table 5A

No, Name

5A-1 PU-RK11

8-((7-iodo-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

5A-2 PU-HT165

8-((7-iodo-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9- (2-(isobutylamino)ethyl)-9H-purin-6-amine

PU-HT175

8-((7-iodo-2,3-dihydroben2o[b][l,4]dioxin-6-yl)thio)-9- (2-(neopentylamino)ethyl)-9H-purin-6-amine

9-(3-(lH-imidazol-l-yl)propyl)-8-((7-iodo-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-purin-6-amine

PU-DZ3-73

2-fluoro-8-((7-iodo-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)me1iyl)-9-(2-(isobu1ylamino)ethyl)-9H-purin-6-amine

PU-DZ4-84

9-(2-(tert-butylamino)ethyl)-2-fluoro-8-.((7-iodo-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-9H-pttrin-6- aniine

9-(3-aminopropyl)-8-((7-iodo-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-purin-6-amine

9-(2-aminoethyl)-8-((7-iodo-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-p rin-6-amine

9-(3-(tert-butylamino)propyl)-8-((7-iodo-2,3- dihydrobenzo [b] [ 1 ,4]dioxin-6-yl)ihio)-9H-puiin-6-amine

1 -(6-amino-8-((7-iodo-2,3-dihydrobenzo[b] [ 1 ,4]dioxin- 6-yl)thio)-9H-purin-9-yl)-3-(isopropylamino)propan-2-ol

5-(6-amino-8-(7-iodo-2,3-dihydrobenzo[b][l,4]dioxin-6- ylthio)-9H-purin-9-yl)pentane-l -sulfonamide l-(3-(6-amino-8-(7-iodo-2,3- dihydroben2o[b] [ 1 ,4]dioxin-6-ylthio)-9H-purm-9- yl)propyl)pyrrolidin-3 -one

6 6-amino-8-(7-iodo-2,3-dihydroben2o[b] l,4]dioxin-6- ylthio)-9H-purin-9-yl)hexanamide

9-(3-(tert-butylamino)propyl)-2-fluorO"8-((7-iodo-2₃3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-9H-purin-6- amine l-(3-(4-(6-amino-8-(7-iodo-2,3- dihydrobenzofb] [ 1 ,4]dioxin-6-ylthio)-9H-purin-9- yl)butyl)pyrrolidin- 1 -yl)ethanone

2-fluoro-8-((7-iodo-2,3-dihydrobenzo[b][l,4]dioxin-6- yl)methyl)-9-(3-(isopropylamino)propyl)-9H-purin-6- amine l-(3-(6-amino-2-fluoro-8-((7-iodo-2,3- dihydrobenzo[b][l,4]dioxin-6-yl)methyl)-9H-purin-9- yl)propyl)pyrrolidin-3 -one

6-(6-amino-2-fluoro-8-((7-iodo-2,3- dihydrobenzo [b] [ 1 ,4] dioxin-6-yl)methyl)-9H-purin-9- yl)hexanamide

5-(6-amino-2-fluoro-8-((7-iodo-2,3- dihydrobenzo[¾][l,4]dioxin-6-yl)methyl)-9H-purin-9- yl)pentane- 1 -sulfonamide

Table 5B

Mo. Name

H-X HJP18

8-((7-(fiiran-2-yl)-2,3-dihy<kobenzo[b][^_^

Durin-6-amine

B-2 1 -(3-(6-amino-8-{7-(oxazol-2-yl)-2,3-dihydrobenzo[b][ 1 ,4]dioxin-6-ylthio)-9H-purin-9- yl)propyl)pyrrolidin-3 -one

B-3 9-(3-(isopropyIamino)propyl)-8-((7-(5-methyli^ yl)thio)-9H-purin-6-amine

5B-4 6-(6-amino-2-fluoro-8-((7-(5-me&y^

9H-purin-9 -yljhexanamide

5B-5 6-(6-arnino-8-(7-(5-methyloxazol-2-yl)-2,3-dih^

yl)hexanamide

5B-6 2-fiuoro-8-((7-(5-meihylfuran^

(methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine

5B-7 TT-VI-53A

2-fluoro-8-((7 furan-2-yl)-2,3-dihyd -obeiizo[b][l,4]dioxin-6-yl^

(isobuiylamino)etuyl)-9H-purin-6-amine

5B-8 l-(3-(2-(6-amino-2-fluoro-8-((7-(5-met^

yI)methyI)-9H-p rin-9-yI)ethyl)piperidin- 1 -yljethanone

5B-9 8-(7-(5-methylftiran-2-yl)-2,3-dihy

(meihyisu]fonyl)piperidin-3-yi)ethyl)-9H-purin~6-ai!Tine

5Β-ΪΘ 2-fluoro-9-(2-(isobutylamino)e(¾yl)-8-((7-(5-methylnu:an-2-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methy!)-9H-pⁱarin-6-amine

5B-11 1 -(3-(6-arnino-2-fluoro-8-((7-(5-methyloxazol-2-yl)-2,3-dihydrober!Zo[b] [ 1 ,4]dioxin-6- yl)methyl)-9H-purin-9-yl)propyl)pyrrolidin-3-Qne

5Β-Ϊ2 8-((7-(5-(aminomethyl)furan-2-yl)-2,3 -dihydrobenzo [b][l,4]dioxin-6-yl)m

(isobutylamino)ethyl)-9H-piirin-6 -amine

SB-13 8-(7-(5-methyloxazol-2-yl)-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)-9-(2-

(neopentylamino)ethyl)-9H-purin~6-arnine

5B-14 l-(3-(2-(6-amino-8-(7-(5-methylfura^^

purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

5B-15 8-((7-(5-raethylfuran-2-yl)-2,3-dihydxobenzo[b][l,4]dioxin-6-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

5B-16 8-((7-(isoxazol-4-yl)-2,3-dihydrobenzo[b][l₅4]dioxin-6-yl)thio)-9-(2-(neopentylami

9H-purin-6 -amine

SB-17 l-(3-(2-(6-amino-8-(7-(5-methyloxazol-2-yI)-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)-9H^ purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

5B-18 8-((7-i5-(aminomethyl)furan-2-y^

(neopentylamino)ethyl)-9H-purin-6-amine

5B-19 5-(6-amino-8-(7-(5-methylfuran-2-yl)-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)

yl)pentane- 1 -sulfonamide

5B-20 5-(6-armno-8-(7-(5-methyloxazol-2-yl)-2,3-dihydroberizo[b][l,4]dioxm-6-ylthio)-9H-^ yl)pentane- 1 -sulfonamide

5B-21 l-(4-(2-(6-amino-2-chloro-8-((7-(furan-2-yl)-2,3-dihyckobe-izo[b][l,^

purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

5B-22 l-(4-(2-(6-aimno-8-((7-(5-methyli½an-2-yl)-2,3-dihy(kobenzo[ ][l_>4]di

purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

5B-23 l-(4-(2-(6-amino-2-fluoro-8-((7-(5-^^

yl)methyl)-9H-purm-9-yl)ethyl)piperidin-l-yl)ethanone

5B-24 l-(3-(6-ammo-8-(7-(5-methyIfean-2-yI)^

yl)propyl)pyrrolidin-3 -one

5B-25 9-(3-(tert-butylaimno)propyl)-8-(7-(5-^

ylthio)-9H-purin-6-amine

5B-26 2-chloro-8-((7-(5-methyliiiran-2-yl)-2 ^

(methylsulfonyl)pyrrolidin-3-yl)ethyl)-9H-purin-6-anaine

5B-27 l-(3-(2-(6-amino-8-(7-(5-(amin^

9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone 5H-28 l-(3-(2-(6-amino~8-((7-(5-(aminoniethyl)furan-2-y )-2,3-dih

yl)methyl)-2-fluoro-9H-purin-9-yI)ethyl)piperidin-l-yl)ethanone

5B-29 5-(6-arrino-2-fluoro-8-((7-(5-methyl^

9H-purin-9-yl)pentane-l-sulfonainide

5B-30 4-(2-(6-arnino-2-chloro-8-i(7Hisoxazo

purm-9-yi)ethyi)piperidine- 1 -carbaldehyde

5B-31 N-(2-((2-(6-armnQ-8-((7-(furan^

yl)ethy1)amino)ethyl)sulfamide

SB-32 3-((2-(0-amino-8-((7-(fean-2-yl)-2^^

yl)ethyi)amino)-N-hydroxypropanamide

5B-33 HJP23

8-((7-(fiiran-2-yl)-2,3-dihydrobenzo^

purin-6-amine

5B-34 HJP20

9-(3 -(isopropylamino)propyl)-8 -((7 -(oxazol-2-yl)-2,3 -dihydrobenzo [b] [ 1 ,4] dioxin-6-yl)thio)- 9H-purin-6-arnine

SB-35 9-(3-aminopropyi)-2-fluGro-8-((7-(5^

y])methyl)-9H-purin-6-amine

5B-36 9-(3-aminGpropyI)-8-(7~(5-methyifc

p rin-6 -amine

SB-37 9-(3-(tert-butylamino)propyl)-8-(7-(5-methyl^

ylthio)-9H-purin-6-amine

5B-38 9-(3-aminopropyl)-8-((7-(oxazol-2-yI)-2 -dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9H

amine

5B-39 9^3-(teri-butyIainino)propy

9H-purm-6-amine

5B-40 9-(3-(terl-butylainino)propyl)^

yl)thio)-9H-piirjr_J-6-amine

SB-41 l-(4-(2-(6-ainino-8-((7 5-me ^^

purin-9-yl)eihyl)piperidin- i -yl)ethanone

5B-42 8-((7 5-rnethyloxazo3-2-yl)^

(methylsuifonyl)piperidm-3-yl)ethyl)-9H-purin-6-amine .

5B-43 1 -(^'6 -amino-8-((7-(5 -methyIfuran-2-yl)-2,3 -dihydrobenzo [b] [ 1 ,4] dioxin-6 -yl)thio)-9H-purin-9- yl)-3-(isopropylamino)propan-2-oi

5B-44 l~(6-amino-8-((7-(5~methyloxazol-2-yl)-2,3-dihydrobe!izo[b][l,4]dioxin-^

yl)-3 -(isopropylamin.o)propan~2 -ol

5B-45 6-(6~amino-2-fluoro-8-((7-(5-methyI¾^

9H-purin-9-yl)hexanamide

SB-46 N-(3-(6-amino-8-((7-(5-methyloxazol-2-yl)-2,3-dihydrobeiizo[b][l,4]dioxin-6-yl)tM

purin-9-yl)propyl)methanesulfonamide

SB-47 l-(2-(4-(0-ammo-8-(7 5-methyto

piixin-9-yl)butyl)pyrrolidin~ 1 -yljethanone

SB-48 9-(3-aimnopropyI)-2-fluoro-8-^

yl)methyl)~9H~p riii-6-ainine

5B-49 2-fl oiO-9-(3-(isopropykmino)propyl)-8-((7-(oxazol-2-yl)-2 -dihydrobenzo[b] [ 1 ,4]dioxin-6- yI)ir₁ethyl)-9H-purin-6-amine

5B-50 2-fluoro-9-(3-(isopropylamino)propyI)-8-((7-(5-methyloxazol-2-yl)-2,3~

dihydrobenzo[l3][l,4]dioxiri-6-yl)meiliyl)-9H-purin-6-amine

5B-51 9-(3 -(iert-butyiamino)propyl)-2 -fluoro-8 -((7-(oxazol-2-yl)-2 ,3 -dihydrobenzo[b][l,4]dioxra-6- yl)metihyl)-9H-purin-6-ainine B-52 9-(3-(tert-butylamino)propyl)-2-fluoro-8-((7-(5-methyloxazol-2-yl)-2,3- dihydrobenzo[¾]fl,4]dioxin-6-yl)methyl)-9H-piirin-6-amine

B-53 6-(6-aniiho-2-fluoro-8-((7-(5-me1ftyloxa^

9H-purin-9-yl)hexanamide

B-54 5-(6-amino-2-fluoro-8-((7-(5-methyloxazol-2-yl)-2,3^ihydrobenzo[b][l,4]dioxin-

9H-purin-9-yl)pentane- 1 -sulfonamide

B-55 l-(3-(6-arnino-2-fluoro-8-((7-(5-me1hyto^

yl)methyl)~9H-p^,arin-9-yl)propyl)p Trolidin-3 -one

B-56 l-(3-(6-amino-2-fluoro-8-((7-(oxazol-2-yl)-2,3-dihydrobenzo|¾][l,4]dioxin-6-yl)m

purin-9-yl)propyl)pyrrolidin-3-one

Table 5C

^' No. Name

5C-1 9-(3-{isoprQpylairjno)propyl)-8-((7-(&^

yl)thio)-9H-purin-6-amine

SC-2 2-fluoro-9-(2-(isobutylainino)etb.yl)-8-((7-(thiophen-2-yl)-2,3- dihydrobenzo b] [ 1 ,4]dioxin-6-yl)methyl)-9H-purin-6-aniine

5C-3 9-(2-(neopentylamino)ethyI)-8-((7-(thiophen-2-yl)-2,3-cHhydrobenzo[bj [ 1 ,4]dioxin-6- yi)thio)-9H-purin-6-armne

5C-4 9-(3-(isopropylamino)propyl)-8-((7-(thiophen-3-yl)-2,3-dihydroben2o[b][l;4]dioxin-6- yl)thio)-9H-purin-6-amine

5C-5 2-fluoro-9-(2-(isobutylamino)ethyl)-8-((7-(thiophen-3-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-9H-purin-6-aniine

5C-6 9-(2-(neopentylamino)ethyl)-8-((7-(thiophen-3-yl)-2_^

yl)thio)-9H-pur½-6-amine

5C-7 l-(4-(2-(6-amino-8-((7-(thiophen-2^

purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

SC-8 4-(2-(6-airrino-8-((7-(thiophen-2-yl)-2,3-ctih^

purin-9-yl)ethyl)piperidine- i -carbaldehyde

5C-9 l-(4-(2-(6-amino-2-fluoro-8-((7-(tlii^^

yl)methyl)-9H-purin-9-yl)ethyl)piperidin-l-yl)ethanone

5C-I1 4-(2-(6-arnino-8-((7-(thiophen-3-yl)-2,3-d&^

purin-9-yl)ethyl)piperidine- 1 -carbaldehyde

5C-13 4-(2-(6-arj_dno-2-chloro-8-((7-(thiopta

yl)methyl)-9H-purin-9-yl)ethyl)piperidine-l -carbaldehyde

5C-14 N-(2-((2-(6-amino-8-((7-(lhiophen-2-yl)-2,3-dihydroberKo[b][l,4]dioxin-6-yl)thio)-9H purin-9-yl)ethyl)amino)ethyl)sulfamide

3-((2-(6~amino-8-((7-(thiophen-2-yl)-2,3-dihydrobeiiZo[b][l,4]dioxin-6-yl)thio)-9H- purin-9-yl)ethyl)aimno)-N-hydroxypropanarnide

5C-16 9-(3-a^'minopropyl)-8-((7-(thiophen-2-yl)-2,3-dihydroberizo[b][l_J4]dioxin-6- yl)thio)-9H-purin-6-amine

5C-17 9-(3-(isopropylamino)propyl)-8-((7-(5-meihylihiophen-2-yl)-2,3- dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9H-purin-6-arnine

5C-18 l-(6-amino-8-((7-(5-methylthiophen-2-yl)-2,3-dih^

9H-purin-9-yl)-3-(isopropylamino)propan-2-ol

5C-19 H2-(3-(6-amino-8-(7-(5-methyltto

9H-purin-9-yl)propyI)pyrrolidin- 1 -yl)ethanone

Table 5D

butylamino)propyl)-9H-p mn-6-amine 5D-19 8-((7-(lH-pyrazol-3-yl)-2,3-dihy<lro^

buiylamino)propyI)-2-fliioro-9H-purin-6-amine

5D-20 9-(3-(isopropylamino)propyl)-8-((7-(5-methyl-lH-pyrazol-3-yl)-2,3- dihydrobenzofb] ί 1 ,4] dioxin-6-yl)thio)-9H-purin-6-amine

5D-21 8-((7-(5-methyl-lH^yrazol-3-yl)-2,3-dihydrobenzo[b][l,4Jdioxin-6-yl)thio)-9-(2

(neopentylamino)ethyl)-9H-purin-6-amine

5D-22 9-(3-(tert-butylamino)propyl)-2-fluoro-8-((7-(5-methyl-lH-pyrazol-3-yl)-2,3- dihydrobeiizo[b] Π ,4]dioxin-6-yl)methyl)-9H-purin-6-amine

5D-23 1 -(8-((7-(lH-pyrazol-3-y!)-2,3-dihydrobenzo[b] [1 ,4]dioxin-6-yl)thio)-6-amino-9H- purin-9-yl)-3-(isopropylamino)propan-2-ol

5D-24 1 -(8-((7-(lH-pyrazol-3-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-6-amino-2- fluoro-9H-purin-9-yl)-3-(tert-butylamino)propan-2-ol

5D-25 5-(8-((7-(lH-pyrazol-3-yl)-2,3-dihy(kobeiizo[b][l,4]dioxin-6-yl)methyl)-6-amm fluoro-9H-purin-9-yl)pentane- 1 -sulfonamide

5D-26 6-(8-((7-(lH-pyrazol-3-yl)-2,3^ihydrobei^

fluoro-9H-purin-9-yl)hexanamide

SD-27 5-(8-(7-(lH-pyrazol-3-yl)-2,3-dithydrobenzo[b][l,4]dioxin-6-ylthio)-6-a-iiino-9H^

9-yl)pentane- 1 -sulfonamide

5D-28 6-(8-(7-(lH-pyrazol-3-yl)-2,3-dihyclroben^

9-yl)hexanamide

Table SE

No. Name

5E-1 8-((7-ethynyl-2,3-dihydrobenzo[b] [ ί ,4]dioxin-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

5E-2 3-(3-(6-ainino-8-(7-ethynyl-2,3-dihy(kobenzo[b][l,4]dioxm-6-ylthio)-9H- purin-9-yl)propyl)pyrrolidine- 1 -carbaldehyde

5E-3 8-((7-ethynyl-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)methyl)-2-fluoro-9-(2-

(isobutylamino)ethyl)-9H-pitrin-6-araine

5E-4 9-(2-anunoe1iiyl)-8-(7-ethynyl-2,3-dihydro^

amine

5E-5 8-((7-ethynyl-2,3 -dihydrobenzofb] [ 1 ,4]dioxin-6-yl)thio)-9-(2-

(neopentylamino)eihyl)-9H-purin-6-amine

5E-6 9-(2-aminoethyl)-8-((7-ethynyl-2,3 i¾^

6-amine

5E-7 1 -(3 -(2 -(6-amino-8 -(7-e thynyl-2 ,3 -dihydrobenzo [b 1 [ 1 ,4] dioxin-6-ylthio)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone

5E-8 8-(7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4] dioxin-6-ylthio)-9-(2-( 1 -

(methylsulfonyrjpiperidin-3-yl)eLhyl)~9H-purin~6-amine

5E-9 8-((7-eihynyl-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)methyl)-2-fluoro-9-(2-(l- (methyisulfonyl)piperidin-3-y])ethyi)-9H-purin-6-araine

SE-10 l-(3-(2-(6-amino-8-((7-ethynyl-2,3^

fluoro-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

5E-11 3-(2-(6-an_dno-8-((7-et ynyl-2,3-dihy(kobenzo[b][l,4]dioxin-6-yl)meth^

9H-purin-9-yl)ethyl)piperidine-l-carbaldehyde

5E-12 l-(3-(6-ainino-8-((7-ethynyl-2,3-dihydro^

9H-purin-9-yl)propyl)pyrrolidin-3-one

5E-13 6-(6-amino-8-((7-ethynyl-2 -dihydro^

purin-9-yl)hexanamide 5E-14 N-(2-((2-(6-amir 8-((7-ethyT^

purin-9-yl)ethyl)amino)ethyl)sulfamide

5E-1S 3-((2-(6-amino-8-((7-ethynyi-2,3-dihydroben2o[b][l,4]dioxin-6-yl)ihio)-9H

yl)ethyl)amino)-N-hydroxypropanamide

5E-16 9-(3-aminopropyl)-8-((7-ethynyl-2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)thio)-9H- purin-6-amine

6-(6-amino-8-(7-ethynyl-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)-9H-purin-9- yl)hexanamide

5E-18 5-(6-amino-8-(7-ethynyl-2,3-dihydiobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9H-purin-9- yl)pentane- 1 -sulfonamide

5E-19 l-(6-ajmno-8-((7-ethynyl-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)methyI)-2-fluoro-9H- purin-9-yl)-3-(tert-butylamino)propan-2-ol

5E-20 l-(6-ainmo-8-((7-ethynyl-2,3-dihydrobei^^

3-(isopropylamino)propan-2-ol

5E-21 5-(6-amino-8-((7~ethyiiyl-2,3-dihydrobenzo[b][l,4]dioxm-6-yl)methyl)-2-fi^

purin-9-y].)peniane- 1 -sulfonamide

5E-22 8-((7-ethynyl-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)methyl)-2-fluoro-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

5E-23 9-(3-(tert-bulylamino)propyl)-8-((7-ethynyl-2 -dihydroberizo[b][l,4]dioxin-6- yl)methyI)-2-fluoro-9H-purin-6-amine

5E-24 9-(3-amnopropyI)-8-((7-ethynyl-2,3-dihydroberKo[b][l,4]dioxin-6-yl)niethyl)-2- fluoro-9H-purin-6-amine

5E-25 9-(3-(tert-butylamino)propyl)-8-(7-ethynyl-2,3-dihydrobenzo[b][l,4]dioxin-6-yltiii

9H-p rin-6-amine

5E-26 8-(7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9-(2-( 1 -methylpiperidin-2- yl)ethyl)-9H-purin-6-amine

5E-27 8-(7-ethynyl-2 -dihydroben2o[b][l,4]dioxin-6-ylthio)-9-(2-(l-methylpiperidin-3- yl)ethyl)-9H-purin-6-amine

5E-28 8-((7-ethynyl-23-dihyckobenzo[b][l,4]dioxin-6-yl)metihyl)-2-fluoro-9-(2-(l^

methylpiperidm-2-yl)ethyl)-9H-purin-6-ainine

5E-29 8-((7-e1it}oiyi-2,3-dihydro^

methylpiperidin-3-yi)ethyl)-9H-purin-6-amine

Table 5F

No. Name

5F-1 7-((6-amino-9-(3-(isopropylamino)propyl)-9H-purin-8-yl)thio)-2,3- dihydrobenzo[b] [ 1 ,4]dioxine-6-carbonitrile

5F-2 7-((6-amino-9-(3-(isopropylamino)propyI)-9H-purin-8-yl)thio)-2,3- dihydrobenzofb] [ 1 ,4]dioxine-6-carbonitrile

5F-3 7-((6-amino-2-fluoro-9-(2-(isobutylamino)ethyl)-9H-purin-8-yl)meth^

dihydrobenzo[b] [ 1 ,4]dioxine-6-carbonitrile

SF-4 7-((6-amino-2-fiuoro-9-(2-(isobutylamino)ethyl)-9H-pijrin-8-yi)TnethyI)-2,3- dihydrobenzo[b] [ 1 ,4]dioxine-6-carboriitrile

5F-S 7-((6-amino-9-(2-(neopen1ylamino)ethyl)-9H-pixrin-8-yl)thio)-2,3- dihydrobenzo [b] [ 1 ,4] dioxine-6-carbonitri le

5F-6 7-((6-aiffiHO-9-(2-(neopentylamino)ethyi)-9H-piirin-8-yl)thio)-2,3- dihydrobenzofb] [ 1 ,4]dioxine-6-carbonitrile

5F-7 2-(7-((6-ainino-2-fiuoro-9-(2-(isobuiylamino)ethyl)-9H-purin-8-y3)methyi)-2,3- dihydrobenzo[b][l,4]dioxin-6-yl)acetonitrile F-8 N-(2-((2-(6-amino-8-((7-(cyaiion:iethyl)-23-dihydrobenzo[b][l,4]dioxin- 9H-pi!iin-9-yI)ethyl)aimno)ethyl)sulfamide

F-9 3-((2-(6-amino-8-((7-(cyanomethyl)-2,3-dihydrobeiizo[b][l,4]dioxin-6-yl)thio)-9H- purin-9-y1)ethyi)amino)- -hydroxypropanaraide

F-10 7-((6-amino-2-chloro-9-(2-(isobu1ylamino)ethyl)-9H-purin-8-yl)methyl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxine-6-carbonitrile

Table 5G

No. Name

5G-1 8-((7-(aziridin-l-yl)-2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)ihio)-9-(2- (neopentylarnino)ethyl)-9H-purin-6-amine

SG-2 8-((7-(azeHdin-l-yl)-2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)thio)-9-(2-

(isobut ^riamino)ethyl)-9H-purin-6-amine

5G-3 9-(2-(isobuiy]amino)ethyl)-8-(^

yl)ihio)-9H-purin-6-amine

5G-4 8-((7-(aziridin-l -yl)-2,3-dihydrobenzo[b][ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9-(2- (neopentylaimno)ethyi)-9H-puriii-6-airiine

5G-5 8-((7-(azeiidin-l-yl)-2 -dihydrober^^

(isobu1ylamino)ethyl)-9H-purin-6-amine

5G-6 2-fluoro-9-(2-(isobu1ylamino)e1hyl)-8-((7-(pyrTolidin-l-yl)-2,3- dihydrobenzo[b^'|[l,4]dioxin-6-yl)methyl)-9H-purin-6-amine

5G-7 2-chloro-9-(2-(isobutylamino)ethyl)-8-((7-(pyrrolidin-l-yl)-2,3- dihydrobeTXZo[bl[l,4]dioxin-6-yl)methyl)-9H-purin-6-amir!e

5G-8 l-(4-(2 6-ammo-8^(7-(a2irid^ .

purin-9-yl)ethyl)piperidiii- 1 -yi)ethanone

5G-9 l-(3-(2-(6-attiino-8-(7-(azeti^

purin-9-yl)et vl)piperidin- 1 -yl)ethanone

SG-10 4-(2-(6-amino-2-chloro-8-((7r( y-Tolidin-l-yl)-2,3-dihydrobenzo[b][l,4]dioxin- yI)methyl)-9H-puriii-9-yl)ethyl)piperidine-l-carbaldehyde

5G-11 8-((7-(dime1hylamino)-2,3-di ydrobenzo[b][l,4]dioxin-6-yl)thio)-9-(2-

(isobutylarnino}ethyr)-9H-purin-6-arnine

5G-12 8-((7-(dimethylamino)-2,3-dihydro^

(isobu lamino)ethyl)-9H-puriii-6-amine

SG-13 l-(3-(2-(6-amino-8-(7-(dimethylami^

purin-9-yl)ethyl)pipertdin- 1 -yl)ethanone

5G-14 1- (3-(2-(6-amino-8-((7-{dimethylaxnino)-2,3-dihydrobenzo[b][ l ,4]dioxin-6-yl)me

2- fluoro-9H-purin-9-yl)ethyl)piperidin-l-yl)ethanone

SG-15 8-(7-(dimethylainino)-2_>3-dihydrobeiizo[b][l ,4]dioxin-6-yllluo)-9-(2-(l- (methylsulfonyl)piperidin-3-yl ethyl)-9H-purin-6-amine

SG-16 8-((7-(dimethylamino)-23-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9-(2-(l- (methylsulfonyl)piperidiii-3-yl)ethyl)-9H-purin-6-arnine

5G-17 8-((7 ethylamino)-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9-(2-

(isobutylamino)ethyl)-9H-purin-6-amine

5G-18 l~(3-(2~(6~amino-8-(7-(ethylainino)-2,3-dihydrobenzo[b][l,4]dioxin-6-ylihio)~9

purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

SG-19 8-(7 ethylamino)-2,3-dihydroberao[b][l ,4]dioxin-6-ylthio)-9-{2-(l- (methylsul fonyl)piperi din-3 -y l)ethyl)-9H-purin-6-amine

SG-20 2-fluoro-9-(2-(isobutylanuno)ethyl)-8-((7-(isopropylamino)-2,3- dihydrobenzorb][I ,4]dioxin-6-y^'i)methyl)-9H-pvirin-6-amine

SG-21 8-(7-(isopropylamino)-2,3-dihydrobeiizo[b][l,4]dioxin-6-ylthio)-9-(2-(l- (melhylsulfonyl)piperidin-3-yl)ethyl)-9H-piirin-6-amine

5G-22 l-(3-(2-(6-amino-2-fluoro-8-((7-(isopropylamino)-2,3-dihyckobenzo[b][l,4]dioxin-6- yl)methyl)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

5G-23 8-((7 dime1hylamino)-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine 5G-24 8-((7-(dimethylammo)-2,3-dihydrob^

(methylsuIfonyI)piperidin-3-yl)ethyl)-9H-purin-6-amine

5G-2S 5-(6-amino-8-(7-(dimethylamino)-2,^

9-yl)pentane- 1 -su] fonamide

5G-26 6-(6-amino-8-(7-(dimethylamino)-2,3^

9-yl)hexanamide

5_G_₂₇ 9-(3-(tert-butylamino)propyl)-8-((7-(di^

6-yl)thio)-9H-purin-6-amine

5G-2S 1 -(3-(6-amino-8^7-(dimethylaniino)-2,3-dihydrobenzo[b][ 1 ,4]dioxin-6-ylthio)-9H- purin-9-yl)propyl)pyrrolidin-3 -one

Table 5H

No. Name

SH-l 8-((7-cyclopropyl-2,3-dihyckobenzo[b][l,4]diox^^

9H-purin-6-amine

5H-2 8-((7-cyclobutyi-23-dihydrobenzo[b][l,4]dioxin-6-yl)thio)-9-(2~(isobutylamino)ethyI)-9 purin-6-amine

5H-3 8-((7-cyclopentyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine

5H-4 8-((7-cyclopropyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

5H-5 8-((7-cyclobutyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9-(2-

(isobutylamino)ethyl)-9H-purin-6-amine

5H-6 8-((7-cyclopentyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9-(2- (isobutylammo)ethyl)-9H-purin-6-amme

5H-7 l-(4-(2-(6-amino-8-((7-cyclopropyl-2,3-dihyc -obenzo[b][l,4]dioxin-6-yl)thio)-9H

yl)ethyl)piperidin- 1 -yl)ethanone

5H-8 l-(4-(2-(6-amino-8-((7-cyclopropyl-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)methyl)-2-fluoro- 9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

5H-9 l-(4-(2-(6-amino-2-chloro-8-((7-cycto^

purm-9-yl)ethyl)piperidin- 1 -yl)ethanone

5H-10 4-(2-(6-arnino-8-((7-cyciobuiyl-2,3-dihydrobenzo[b][l₅4]dioxin-6-yl)t

yl)ethyl)piperidine- 1 -carbaldehyc!e

5H-11 4-(2-(6-amino-8-((7 :yclopentyl-2,3-dihyd^^

purin-9-yl)ethyl)piperidine-l-carbaldehyde

511-12 3-(6-amdno-8-((7-cyclopropyl-2,3-dihyckobenzo[b][l,4]dioxin-6-yl)1±io)-9

yljpropyl sulfamate

SH-13 8-((7-cyclopropyl-2,3 -dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(2-( 1 - (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine

5H-14 9-(3 -(tert-butylamino)propyl)-8-((7-cyclopropyl-2,3 -dihydrobenzo[b] [ 1 ,4]dioxin-6-yi)thio)~ 9H-purin-6-amine

5H-15 2-(3-(6-arnino-8-(7-cyclobu1yl-2,3-dihyclro^

yljpropyljpyrroiidine- 1 -sulfonamide

5H-16 3-(2-(6-arnmo-8-(7-cyclopentyl-2₃3-dihydrobenzo[b][!,4]dioxin-6-yItb^

yl)ethyl)pyrrolidine- 1 -sulfonamide

5H-17 8-(7-cyclopentyl-2,3 -dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9-(2-( 1 -(methylsulfonyl)piperidin- 3-yl)ethyl)-9H-purin-6-amine

5H-18 9-(3-(tert-butylamino)propyl)-8-(7-cyclo^ purin-6-amine

Η-Ϊ9 1 -(6-amino-8-(7-cyclopentyl-2,3 -dihydrobenzo[b] [ 1 ,4] dioxin-6-ylthio)-9H-purin-9-yl)-3 -

(isopropyIamino)propan-2-ol

Table 6A

No. Name

6A-1 8-((7-iodo-2 -dihydrobenzo[b][ I,4]oxat

9H-purin-6-amine

6A-2 8-((7-(ft ran-2-yl)-2,3-dihydrobenzo[b][l,4]oxathiin-6-yl)thio)-9-(

(isopropylamino)propyi)-9H-purin-6-amine

6A-3 8-((7-ethynyl-2,3-dihydrobenzo[b][l ,4]oxathiin-6-yl)thio)-9-(3-

(isopropyiamino)propyi)-9H-purin-6-amine

6A-4 4-(2-(6-amino-8-((7-(faran-2-yl)-2,3-di^^

9-yl)ethyl)piperidine- 1 -carbaldehyde

6A-5 2-fluoro-9-(2-(isobu1ylamino)e l)-8-((7-(pyrrolidin-l-yl)-2,3- dihydrobenzo[b] [ 1 ,4]oxathiin-6-yl)methyl)-9H-piirin-6-amine

6A-6 8-((7-(aziridin-l -yl)-2,3-dihydrobenzo[b][l ,4]oxathiin-6-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine

6A-7 8-((6-(furan-2-yl)-2,3-dihydrobeiizo[b][l ,4]oxathiin-7-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

6A-8 8-((6-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]oxathiin-7-yl)thio)-9-(3-

(isopropylamino)propyi)-9H-purin-6-aniine

6A-9 4-(2-(6-amino-8-((6-(furan-2-yl)-2,3^

9-yl)ethyl)piperidine- 1 -carbaldehyde

6A-10 2-fluoro-9-(2-(isobutylamino)ethyl)-8-((6-(pyrrolidin-l-yl)-2,3- dihydrobenzofb] [ 1 ,4]oxathiin-7-yl)methyl)-9H-purin-6-amine

6A-11 8-((6-(aziridin-l -yl)-2,3-dihydiobeiizo[b][ l,4]oxatiiiin-7-yl)thio)-9-(2-

(neopent 'lam.ino)ethyl)-9H~purin-6-amiiie

6A-12 8-((7-(furan-2-yl)-4-methyl-3,4-dihydro-^^

(neopentyiamino)ethyl)-9H-purin-6-arrjine

6A-13 8-((4-methyl-7-(lH-pyrazol-3-yl)-3,4-dihydro-2H-beiizo[b][l ,4]oxazin-6-yl)thi

(neopentylainino)ethyl)-9H-purin-6-arnine

6A-14 8-((7-ethynyl-4-methyl ,4-dihydro-2H-ben2X)[b][l_J4]oxazin-6-yl)thio)-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine

6A-15 2-fluoro-8-((7-(r½an-2-y!)-4-methyI-3,4-^^

9-(2-(neopentylamino)ethyl)-9H-purin-6-amine

6A-16 4-(2-(6-amino-8-((7-(fttran-2-yl)-4-methyl-3_>4-dihydro-2H-benzo[b] [ 1 ,4]thiazin-6- yl)thio)-9H-purin-9-yl)ethyl)piperidine- 1 -carbaldehyde

6A-17 8-((6-(furan-2-yl)^-methyl-3,4-dihy(Iro^

(isopropylamino)propyl)-9H-purin-6-amine

6A-18 9-(3~(isopropyiamino)propyl)-8-((4-methyl-6-(thiopheri-2-yl)~3,4-dihydro-2H- benzo[b] [ 1 ,4]oxazm~7-yl)thio)-9H-purin~6-amine

6A-19 4-(2-(6-amino-8-((6-(furan-2-yl)-4-methyl-3,4-dihydro-2H-benzo[b] [ 1 ,4]oxazin-7- yl)thio)-9H-purin-9-yl)ethyl)piperidine- 1 -carbaldehyde

6A-20 4-(2-(6-amino-8-((6-ethynyl-4-methy3-3,4-dihydro-2H-benzo[b][l ,4]oxazin-7-yl)ihio)

9H-purin-9-yl)ethyl)piperidine-l -carbaldehyde

6A-21 8-((6-(azetidin-l -yl)-4-methyl-3,4 U^

(isobutylarrjino)ethyl)-9H-purin-6-amine

9H-purin-9-yl)propyl sulfamate

6A-53 l-(6-amino-8-((4-methyl-6-(5-metiiylo^

7-yl)thio)-9H-purin-9-yl)-3-(isopropylamino)propan-2-ol

6A-54 9-(3-(tert-bu1ylaiiiino)propyl)-8-((4-m

benzo[b][l,4]oxa2in-7-yl)thio)-9H-purin-6-arnine

Table 6B

No. Name

6B-1 8-((3-iodo-5,6,7,8-tetrahydronaph1¾alen-2-yl)thio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine

6B-2 8-((3-iodo-5,6,7,8-tetrahy(lronaphthalen-2-yl)thio)-9-(2-(isobutylardn^

amine

6B-3 8-((3-iodo-5_;6,7,8 etrahydTonaphthalen-2-yl)ih.io)-9-(2-(neopenfylamino)ethyl)- 6-amine

6B-4 2-fluoro-8-((3-iodo-5,6,7,8-tetraliydronaphthalen-2-yl)methyl)-9-(2-(isobu1ylaini

9H-purin-6-amine

6B-5 2-chloro-8-((3-(fuxan-2-yl)-5,6_.J^-teirahydronaphthalen-2-yl)inethyl)-9-(3^

(isopropylamino)propyl)-9H-purin-6-amine

6B-6 2-fluoro-8-((3-iodo-5,6,7,8-tetxahydronaphthalen-2-yl)methyl)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine

6B-7 8-((3-(furan-2-yl)-5,6,7,8-tetrahycto^

9H-purin-6-amine

6B-8 8~((3-ethynyl-5,6,7,8~tetrahyGronaphthalen-2-yl)thio)-9-(3-(isopropylairjno)prop

purin-6-amine

6B-9 3-((6-amino-9-(3-(isopropylamino)propyi)-9H-purin~8-yl)thio)-5 ,6,7,8- tetrahydronaphthalene-2-carbonitrile

6Β-1Θ 8-((3-(azetidin-l-yI)-5,6,7,8-tetrahydronaphthalen-2-yl)methyl)-2-fluoro-9-(2- (neopen1ylamino)ethyl)-9H-purin-6-amine

6B-11 8-((3-iodo-5,6,7,8 etrahydronaphthaIeri-2-yI)thio)-9-(2-(isobuiyiamino)ethyl)-9H amine

6B-12 6-(6-amino-8-(3-(oxazol-2-yl)-5,6,7,8-tetrahydronaphthalen-2-ylthio)-9H-purin-9- yl)hexanamide

6B-13 l-(3-(2-(8-(3-(lH-p3^azoi-3-yl)-5,6,7,8 etrahydronaphthaien-2-yIthio)-6

9-yl)ethyl)piperidin- 1 -yl)ethanone

6B-14 i-(3-(2-(6-amino-8-(3^ihyiiyl-5,6,7,8-tetTahydronaphihalen-2-ylthio)-9H-puiin-9- yl)ethyl)piperidin- 1 -yl)ethanone

6B-15 l-(3-(2-(6-amino-2-fluoro-8-((3-(5-methylfc

yl)methyl)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone

6B-16 N 2-((2-(6-amino-8 (3-(iliran-2-yl)-5,6,7,8 eira ydronaphthalen-2-yl)thi

yl)ethyl)amino)ethyl)meib.anesuIfonanaide

6B-17 3-((2-(6-amino-8-((3-(fiu n-2-y])-5,6,7,8-te^

yl)ethyl)amino)-N-hydroxypropanamide

6B-18 9-(3-aminopropyl)-8-((3-iodo-5,6_J7,8-tetrahydronaph1halen-2-yl)thio)-9H-piirm

6B-19 9-(3-(isopropylannno)propyl)-8-((3-(oxazol-2-yl)-5,6,7,8-tettahydronaphthalen-2-yl)thi

9H-purin-6-amine

6B-20 9-(3-(tert-butylamino)propyl)-8-((3-iodo-5,6,7,84etiahydronaph1±ialen-2-yl)thio)-9H- purin-6-amine

6B-21 l-(3-(2-(6-amino-8-(3-(5-methylfiiran-2-y^

y propy pyrro n- -one

Table 7A

carbaldehyde

Tabie 7B

ethoxybenzonitrile 7B-11 N-(2-(2-(6-amino-8-(2-cyano-5-methoxyphenylthio)-9H-purin-9- yl)ethylamino)ethyi)methanesulfonamide

7B-12 3-(2-(6-ainino-8-(2-cyano-5-methoxyphen^

hydroxypropanamide

Table 7C

yl)ethyl)piperidin- 1 -yljethanone

Table 7D

Table 7E

Table 7F No. Name

F-1 9-(3-(isopropylamino)propyl)-8-(5-methoxy-2-(lH-pyrrol-3-yl)phenyllhio)-9H- purin-6-amine

F-2 9-(3-(isopropylamino)propyl)-8-(5-methoxy-2-(lH-pyrrol-2-yl)phenylthio)-9H- purin-6-amine

F-3 2-fluoro~9-(2-(isobutylaimno)ethyl)-8-(5-meihoxy-2-(lH-pyn:ol-3-yI)benzyl)- 9H-purin-6-amine

F-4 8-(5-methoxy-2-(lH-pyrrol-3-yl)phenyltW

2-yl)ethyl)-9H-purin-6-arnine

F-5 8-(5-methoxy-2-(lH-pyrrol-3-yl)phenylthio)-9-(2-(neopentylamino)ethyl)-9H-

Durin-6-amine

F-6 9-(3-(isopropylamino)propyl)-8-(5-methoxy-2-(5-methyl-lH-pyrrol-3- yl)phenylthio)-9H-purin-6-amine

F-7 4-(3 -(6-amino-8-(5 -methoxy-2 -( 1 H-pyrrol-3 -yl)phenylthio)-9H-purin-9- yl)propyl)piperidine-l-carbaldehyde

F-8 4-(2-(6-amino-8-(5-methoxy-2-(lH-pyrrol-3-yl)phenylthio)-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde

F-9 1 -(4-(2-(6-ammo-2-flaoro-8-(5-methoxy-2-( 1 H-pyrrol-3-yl)benzyl)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone

F-10 l-(4-(3-(6-amino-2-fluoro-8-(5-methoxy-2-(lH-pyrrol-3-yl)benzyl)-9H-piirin-9- yl)propyl)piperidin- 1 -yl)ethanone

F-11 l-(4-(2-(6-arrdno-2-chloro-8-(5-methoxy-2-(lH-pyrrol-3-yl)benzyl)-9H-piirin-9- yl)ethyl)piperidin- 1 -yl)ethanone

F-12 N-(3-(6-amino-8-(5-methoxy-2-(lH-pyrrol-3-yl)phenylthio)-9H-purin-9- yl)propyl)methanesulfonamide

F-13 8-(5-methoxy-2-(lH-pyrrol-3-yl)phenylthio)-9-(2-(l-(methylsulfonyl)piperidin- 3-yl)ethyl)-9H-piirin-6-amine

F-14 N-(2-(2-(6-amino-8-(5-methoxy-2-(lH-pyrrol-3-yl)phenyltiiio)-9H-purin-9- yl)ethylamino)ethyl)sulfamide

F-15 3-(2-(6-amino-8-(5-methoxy-2-(lH-pyrrol-3-yl)phenylthio)-9H-purin-9- yl)ethylamino)-N-hydroxypropanainide

F-16 8-(5-ethoxy-2-(lH-pyrrol-3-yl)phenylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine

F-17 8-(5-ethoxy-2-(lH-pyrrol-3-yl)benzyl)-2-fluoro-9-(2-(isobutylamino)etiiyl)-9H- purm-6-amine

F-18 9-{3 -(tert-butylamino)propyl)-8 -(5 -methoxy-2 -(1 H-pyrrol-3 -yl)phenylthio)-9H- purin-6-amine

F-19 9-(3-arninopropyl)-8-(5-methoxy-2-(lH-pyrrol-3-yl)phenyIthio)-9H-purin-6- amine

F-20 l-(6-amino-8-(5-methoxy-2-(lH-pyiTol-3-yl)phenyithio)-9H-purin-9-yl)-3- (isopropylamino)propan-2-ol Table 8

Options for R.

1 , R is hydrogen, a Q to Cm aikyl, alkenyl, alkynyl, or an alkoxyalkyl group, optionally including eteroatorns such as N or O, or a targeting moiety connected to N9 via a linker,

2. R is hydrogen, straight- or branched-, substituted or unsubstituted aikyl, substituted or unsubstituted alkenyl, substituted or unsubstituted a!kynyl, in which one or more methylenes can be interrupted or terminated by O, S, S(O), S0₂, N{R₂₁g ), C{0), substituted or unsubstituted aryl, substituted or unsubstituted heteroaryi, substituted or unsubstituted heterocyclic; substituted or unsubstituted cycloalkyi; or

B is a linker; R₂₁₀ is selected from the group consisting of hydrogen, N{R₂)COR₄,

N(R₂}CON{R₃)R₄, N(R₂)COOR₄, M{R.₂}S{0}_nR₃, N{R₂)S(0)_nN(R₃)R₄; where R₂ and R₃ are independently selected from hydrogen, aliphatic or substituted aliphatic; R_A is selected from the group consisting of: aryl, substituted aryl, heteroaryi, substituted heteroaryi, heterocyclic, substituted heterocyclic, cyioalkyi, substituted cycloalkyi, cycloaikenyi, substituted cycloa!kenyl, and substituted or unsubstituted -Ci-C₆ aikyl, -C₂-C₆ alkenyl, or -C₂- C₆ alkynyl each containing 0, 1, 2, or 3 heteroatorns selected from O, S or N; n is 1 or 2;

Mi is absent or selected from substituted or unsubstituted -Ci-C₆ alky!, -C₂- C₆ a!keny!, or -C₂-C₆ alkynyl, aryl, substituted aryl heteroaryi, substituted heteroaryi;

M₂ is absent, O, S, SO, S0₂, N(R₂) or CO;

M₃ is absent, O, S, SO, S0₂, N(R₂), CO, Cl-Q aikyl, C₂-Q alkenyl, C₂-C₃ a!kyny!, cycloalkyi, heterocyclic, aryl, or heteroaryi;

M₄ is hydrogen, NR₅R₆, CF₃, OR₄, halogen, substituted or unsubstituted -C C₆ aikyl, -C₂-C₆ alkenyl, or -C₂- C₆aikynyl, cycloalkyi, substituted cycloalkyi, heterocyclic, substituted heterocyclic, aryi, substituted aryl, heteroary! or substituted heteroaryi; where R₅ and R₆ are independently selected from the group consisting of hydrogen, aliphatic, substituted aliphatic, aryl, substituted aryl, heteroaryi, substituted heteroaryi, heterocyciic, substituted heterocyclic, cycloalkyi or substituted cycloalkyi; provided that -R and -Mi-M₂- ₃- M cannot be both hydrogen. Table 8 cont'd

wherein R³ⁱ is

(a) hydro;

(b) C C₆ a!kyl optionally substituted with 1 , 2, 3, 4, or 5 substituents each independently chosen from the group of halo, hydroxyl, amino, cyano, and -C(=0)R³ⁱ wherein R^3~ is amino;

(c) -C(=Q)R^3:i, wherein R³³ is selected from the group consisting of:

(1) hydro,

(2) C C₁₀ (e.g., Ci-Cg) aikyl optionally substituted with 1 , 2, 3, 4, or 5 substituents each independently chosen from the group of (A) ha!o, {B} hydroxy!, (C) thiol, (D) cyano, (E) Ci-Cg haloaikyl (e.g., trifiuoromethyi), (F) C-,-- C_s alkoxy (e.g., methoxy) optionally substituted with Cx-C₃ alkoxy (e.g., methoxy}, (G) C-amido, (H) i-amido, (I) sulfonyl, (jj -N(Rⁱ²)(R²³) wherein R²² and R²³ are independently hydro, C C₆ alky!, sulfonyl, and C-carboxy,

(3) Cj-Cg cycioalkyl optionally substituted with 1 , 2, 3, 4, or 5 substituents each independently chosen from the group of halo, hydroxy!, amino, cyano, and C,-,-C₆ haloaikyl (e.g., trifiuoromethyi), and

(4) C C(5 alkoxy optionally substituted with 1 , 2, 3, 4, or 5 substituents each independently chosen from halo, hydroxyl, amino, cyano, and C C₆ haloaikyl (e.g., trifiuoromethyi),

(f) heterocycle or heterocyc!y!alkyl, optionally substituted with 1 , 2, 3, 4, or 5 substituents independently chosen from halo, hydroxy!, amino, cyano, triha!omethy!, and ^-C^ alky! optionally substituted with 1 , 2, 3, or 4 substituents independently chosen from halo, hydroxy!, amino, cyano, C C₆ ha!oa!ky! (e.g., trifiuoromethyi) (e.g., tetrazole-5-yl optionally substituted with 1 , 2, 3, or 4 C^Q alkyi};

(g) sulfonyl; and

(h) optionally substituted heteroaryl Table 8, cont'd

4. R is -R^="*-R^5S, wherein FT⁴ is -(CH₂)_n- wherein n=Q-3, -C{0}, -C(S), -S0₂-, or -S0₂N-; and

R^dS is aikyi, aromatic, heteraaromatie, alicyclic, or heterocyclic, each of which is optionally bi-or tri-cyciic, and optionally substituted with H, halogen, lower aikyl, lower aikenyl, lower aikyny!, lower aryl, lower alicyclic, ara!kyi, aryloxyalkyi, alkoxyaikyi, perhaioalkyl, perhaloalkyioxy, perhaloacyl, -N₃, -SR^5i!, -O R⁵'⁵, -CN, -C0₂R^5S, - N0₂, or— N R⁵⁸R⁵ⁱ⁰,

R⁵⁸ is hydrogen, lower alkyl, lower aryl, or -C(O) R^5'5;

R⁵⁹ is lower alkyl, lower aryl, lower heteroaryl, -N R⁵¹⁰ R⁵ⁱ⁰ or -OR^{51 1};

R⁵¹⁰ is independently hydrogen or lower alkyl; and

5. R is selected from the group consisting of H, optionaily substituted alkyl, optionally substituted aikenyl, optionally substituted alkynyl, optionally substituted aryl, optionally substituted alicyclic, optionally substituted araalkyi, optionally substituted aryloxyalkyi, optionally substituted alkoxyaikyi, alkylaminoalkyl, alkylcarbonylaniinoalkyl, alkylcarbonyoxylalkyl, optionally substituted heterocyclic, hydroxyaikyl,

6. R is H, SR_{7 I}, SOR-i, S0₂R₇i, OR₇₎, COOR₇₁, CONR₇,R₇₂, -CN, C_w alkyl, C₂.₆ aikenyl, C_2-6 alkynyl, - -RTAORTB- --RTA RTB, -R₇ANR₇IR7B, -.-RTASRTB, --R_7ASOR_7B or -R_7AS0₂RTB, cycloalkyl, heteroaikyl, heterocycloalkyl, aryl, heteroaryl, alkylaryl, arylalkyi, alkylheteroaryl, heteroarylalkyl, NR₇iR₇₂,

--OS0₂N(R_7C)₂, ~N(R_7C)S0₂OH, -N(R₇c)S0₂R_7C, -R_7AOS0₂N(R₇c)2, or -R7_AN(R_7C)OS0₂R_7C;

R₇I and R₇₂ are independently selected from the group consisting of H, COOR_7B, CON(R_7C)₂ C|.₆ aikyl, C₂.₆ aikenyl, C₂.₆ alkynyl, -R_7AOR_7B~, -R_7ANR_7B, -R_7ANR7iR_7B, -R_7ASR_7B, -R_7ASQR_7B or -R_7ASG₂R_7B cycloalkyl, heteroaikyl, heterocycloalkyl, aryl, heteroaryl, alkylaryl, arylalkyi, alkylheteroaryl, and heteroarylalkyl;

each R_7A is independently C_l-6 alkyl, C₂.A aikenyl, C₂.₆ alkynyl, cycloalkyl, heteroaikyl,

heterocycloalkyl, aryl, heteroaryl, alkylaryl, arylalkyi, alkylheteroaryl, aikyiheteroarylalkyl, or heteroarylalkyl; and

each R_7B is independently H, C< _6 alkyl, C₂_6 aikenyl, C_2-6 alkynyl, cycloalkyl, heteroaikyl, heterocycloalkyl, aryl, heteroaryl, alkylaryl, arylalkyi, alkylheteroaryl, heteroarylalkyl, ~S0₂OH,™S0₂N(R_7A)₂, -S0₂NHR_7A or -S0₂NH₂; and

each R.sub.C is independently H, Q.₆ alkyl, C₂._s aikenyl, C₂.₆ alkynyl, cycloalkyl, heteroaikyl, heterocycloalkyl, aryl, heteroaryl, alkylaryl, arylalkyi, alkylheteroaryl, or heteroarylalkyl;

7A. R is hydrogen, straight- or branched-, substituted or unsubstituted alkyl, substituted or unsubstituted aikenyl, substituted or unsubstituted alkynyl, which one or more methylenes can be interrupted or terminated by O, S, S(O), SO?, N(R_S8), C(O), substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocyclic; substituted or unsubstituted cycloalkyl; where Rg₈ is hydrogen, acyl, aliphatic or substituted aliphatic,

7B. R is -M1 -M2-M3-M4, wherein

M; is absent, Cj-C₆ alkyl, C₂-Q aikenyl, C₂-Q alkynyl, aryl or heteroaryl;

M₂ is absent, O, S, SO, S0₂, N(R₈₈), or C=0;

M₃ is absent, C=0, O, S, SO, S0₂ or N(R₈₈); and

Mt is hydrogen, halogen, CN, N₃, hydroxy, substituted hydroxy, amino, substituted amino, CF₃, Q-Cg alkyl, C₂-C₆ aikenyl, C₂-C₆ alkynyl, cycloalkyl, heterocyclic, aryl or heteroaryl.

Claims

compound of the formula:

wherein

(a) each of Zh Z2 and Z3 is independently C or N, with H substituents as needed to satisfy valence;

(b) Xa, Xb and Xc are all carbon, connected by two single or one single bond and one double bond, and wherein

(c) Y is -CH₂- or -S-;

(d) X4 is hydroge or halogen; and

(e) X2 and R in combination are selected from the group consisting of:

(i) X₂ is halogen and R is primary amino-alkyl, a secondary or tertiary alkyl-amino-alkyl, aryl-alkyl, or a nonaromatic heterocycle-alkyl, wherein the amine 's nitrogen and the heterocycle's heteroatom are substituted to satisfy valence, with the proviso that R is not a piperidino moiety; and

(ii) X₂ is selected from the group consisting of alkyl, alkenyl, alkynyl, aryl, cycloalkyl, cycloalkenyl, saturated or unsaturated heterocycle, aryl, aryloxy, alkoxy, halogenated alkoxy, alkenyloxy, hydroxyalkyl, amino, alkylamino, dialkylarnino, acylamino, carbamyl, amido, dialkylamido, aLkylamido, alkylsulfonamido, sulfonamide, tnhalocarbon, - thioalkyl, SC -alkyl. -COO-alkyl, OH or alkyl-CN, or part of a ring formed by R, and

R is a group listed in Table 8.

2. The compound of claim 1 , wherein X₂ is not halogen.

3. The compound of claim 2, wherein X₂ is alkynyl. 109

4. The compound of claim 3, wherein the compound is selected from the group consisting of: 8-((6-ethyiiyl-2 -dihydro-lH-mden-5-yl)thio)-9-(3-(isopropylammo)propyl)- 9H-purm-6-amine; l-(3-(2-(6-amino-8-(6-eth ^^

9-yl)ethyl)piperidin-l-yl)ethanone; l-(3-(3-(6-arnino-8-(6-ethynyl-2,3-dihydro-lH-inden-5- ylthio)-9H-piirin-9-yl)propyl)pyrrolidin-l-yl)ethanone; 8-((6-ethynyl-2,3-dihydro-lH-mden- 5-yl)tMo)-9-(2-(neopentylamino)ethyi)-9H-purin-6-amine; 5-(6-amino-8-(6-ethynyl-2,3- dihydro- 1 H-inden-5-ylthio)-9H-purin-9-yl)pentane~ 1 -sulfonamide; 1 -(4-(3-(6-amino-8-(6- ethywl-2,3-dihydro-lH-inden-5-yl 9-(3- (tert-butylamino)propyl)-8-(6-ethynyl-2,3-dihydro-lH-inden-5-ylthio)-9H-purin-6- 1- acetyl-3-(3-(6-amino-8-(6-emyQyl-2,3-dmydro-lH-inden-5-yltMo)-9H-purin-9-.

yl)propyl)imidazolidin-2-one; 8-((6-eth>^?nyl-2,3-dihydro-iH-mden-5-yl)thio)-9~(2-(i- methylpiperidin-2-yl)ethyl)-9H-purin-6-amine; 8-((6-ethynyl-2,3-dihydro-lH-inden-5- yl)thio)-9-(2-(l-methylpiperidin-3-yl)ethyl)-9H-purin-6-amine; 8-((6-ethynyl-2,3-dihydro- 1 H-inden-5-yl)thio)-9-(2-(l -(methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine; 1 -(3- (2-(6-amino-8-((6-emynyl-2,3-dihydro^

yl)ethyl)piperidin-l-yl)ethanone; 9-(3-(tert-butylamino)propyl)-8-((6-ethynyl-2,3-dihydro- lH-mden-5-yl)methyl)-2-fluoro-9H-purin-6-amine; 6-(6-amino-8-((6-ethynyl-2,3-dihydro- 1 H-inden-5-yl)methyl)-2-fluoro-9H-purin-9-yl)hexanamide; 1 -(3-(6-amino-8-((6-ethynyl- 2,3-dihydro- 1 H-inden-5-yl)memyl)-2-fluQro-9H-purm^ 4-(6- amino-8-((6-ethynyl-2,3 -dihydro- 1 H-inden-5 -yl)methyl)-2-fluoro-9H-purin-9-y] jbutane- 1 - sulfonamide; 8-((6-ethynyl-2,3-dihydTO-lH-inden-5-yl)methyl)-2-fluoro-9-(3- (isopropylammo)propyl)-9H-purin-6-amine; 8-((6-ethynyl-2,3-dihydro-lH-inden-5- yl)memyl)-2-fluoro-9-(2-(neopentylaiiiino)ethyl)-9H-purin-6-amine; 3-(2-(6-amino-8-((6- emynyl-2,3-dihydro-lH-inden-5-yl)meth^

sulfonamide: 8-((6-ethynyl-2,3-dihydro-lH-iiiden-5-yl)methyl)-2-fluoro-9-(2-(l- methylpiperidin-2-yl)ethyl)-9H-purin-6-amine; and 8-((6-ethynyl-2,3-dihydro- 1 H-inden-5- yl)methyl)-2-fluoro-9-(2-(i-methylpiperidin-3-yl)ethyl)-9H-purin-6-am.ine

5. The compound of claim 2, wherein X is heteroaryl.

6. The compound of claim 5, wherein the compound is selected from the group consisting of: 8-((6-(faran-2-yl)-2,3-dihydro-lH-inden-5-yl)thio)-9-(3- (isopropylammo)propyl)-9H-purin-6-amine; 9-(3-(isopropylamino)propyl)-8-((6-(oxazol-2- yl)-2,3-dihydro- 1 H-inden-5-yl)thio)-9H-purin-6-amme; 1 -(3-(2-(6-ammo-8-(6-(oxazol-2-yl)- 2,3-dihydro H-inden-5-yltMo)-9H-pim^ 3-(2-(8-(ό-(1Η- pwazol-3-yl)-2,3-dihydro- 1 H-i de^

carbaldehyde; N-(2-((2-(6-animo-8-((6-(oxazol-2-yl)-2,3-dihy(iro-lH-inden-5-yl)thio)-9H- purin-9-yl)ethyi)amino)ethyl)sulfamide; 3-(2-(6-amino-8-(6-(oxazol-2-yl)-2,3-dihydro-lH- inden-5-ylthio)-9H-purin-9-yl)ethylamino)-N-hydrox\propanamide; 9-(3- (isopropylammo)prQpyl)-8-((0-(5-m

purin-6-amine; 8-((6-(5-methyloxazol-2-yl)-2₅3-dihydro-lH-inden-5-yl)thio)-9-(2-(l- (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine; 9-(3-aminopropyi)-8-((6-(5- methyloxazol-2-yl)-2,3-dihydro-l H-inden-5-yl)tMo)-9H-purin-6-amine; 9-(3-(tert- butylammo)propyl)-8-(6-(4-melJiyllM

amine; 8-((6-(5-methyloxazol-2-yl)-2,3-dihydro- 1 H-inden-5-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine; l-(6-amino-8-((6-(5-methyloxazol-2-yl)-2,3- dihydro-lH-inden-5-yl)tMo)-9H-purin-9-yl)-3-(isopropylamino)propan-2-ol; l-(2-(4-(6- amino-8-(6-(5-methylfuran-2-yl)-2,3-dihydro-lH-mden-5-ylt o)- yi)butyl)pyrrolidin- 1 -yl)ethanone; 1 -(3-(2-(6-amino-8-(6-(5-me1iiyloxazol-2-yl)-2,3-dihydro- 1 H-mden-5-ylthio)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone; 6-(6-amino-8-(6-(oxazol-2- yi)-2₅3-dihydro-l H-inden-5-ylthio)-9H-purin-9-yl)hexanamide; l-(3-(6-amino-8-(6-(4- methyloxazol-2-yl)-2,3 -dihydro- 1 H-inden-5-yit o)-9H-purin-9-yl)propyl)pyrrolidin-3-one; 2-fluoro-9-(3-(l -(methyl sulfonyl)^

inden-5-yl)methyl)-9H-purin-6-amine; l-(3-(2-(6-arnino-2-fluoro-8-((6-(4-methylthiazol-2- yl)-2,3 -dihydro- 1 H-inden-5 -yl)methyi)-9H-purin-9-yl)ethyl)piperi din- 1 -yl)ethanone; 9-(3 - (tert-butylamino)propyl)-2-fliioro-8-((6-(4-methyltMazol-2-yl)-23-dihydro-lH-inden-5- yl)methyl)-9H-purin-6-amine; 8-((6-( 1 H-pyrazol-3-yl)-2,3-dihydro- 1 H-inden-5-yl)methyl)-9- (3-(tert-butylamino)propyl)-2-fluoro-9H-piirin-6-amine; 6-(6-amino-2-fiuoro-8-((6-(oxazol- 2-yl)-2,3-dihydro-lH-inden-5-yl)methyl)-9H-purin-9-yl)hexanamide; l-(3-(6-amino-2- fluoro-8-((6-(oxazol-2-yl)-2,3-dihydro-lH-inden-5-yi)methyl)-9H-p rin-9- yl)propyl)pyrrolidin-3-one; 5-(6-amino-2-fluoro-8-((6-(oxazol-2-yl)-2,3-dihydro-l H-inden-5- yl)methyl)-9H-puxin-9-yl)pentane- 1 -sulfonamide: 2-fluoro-9-(2-( 1 -methylpiperidin-2- yl)ethyl)-8-((6-(oxazol-2-yl)-2,3-dihydro- 1 H-inden-5-yl)methyI)-9H-purin-6-amine; and 2- fluoro-9 2-(l-methylpiperidm-3-yi)e^

yl)methyl)-9H-purin-6-amine.

7. The compound of claim 1 , wherein X₂ is iodine.

8. The compound of claim 7, wherein the compound is selected from the group consisting of: l-(6-amino-8-(6-iodo-2 -dihytko-lH-inden-5-yItmo)-9H-purin-9-yl) hutylainmo)propan-2-ol: 8-((6-iodo-2,3-dihydro- 1 H-inden-5-yl)thio)-9-(2- (isobuiylammo)ethyl)-9H-purin-6-amine: l-(3-(6-arnmo-8-(6-iodo-2₅3-dihydro-lH-inden-5- ylt o)-9H-purin-9-yl)propyl)pyrroiidin-3-one; l-(3-(3-(6-amino-8-(6-iodo-2,3-dihydro-lH- inden-5-ylthio)-9H-purin-9-yl)propyl)pyrrolidiii-l-yl)ethanone; 8-((6-iodo-2,3-dihydro-l H- inden-5-yl)tMo)-9-(2-(neopentylarriino)ethyl)-9H-purin-6-amine: 8-((6-iodo-2,3-dihydro-lH- inden-5-yl)tMo)-9-(3-(isopropylamino)propyl)-9H-purin-6-amine; 9-(3-aniinopropyl)-8-((6- iodo-2,3-dihydro-lH-inden-5-yl)thio)-9H-purin-6-amme; 9-(2-ammoethyl)-8-((6-iodo-2,3- dihydro- 1 H-inden-5-yl)thio)-9H~purin-6-amine; 9-(3-(tert-butylamino)propyl)-8~((6-iodo- 2,3-dihydro-lH-mden-5-yl)thio)-9H-purin-6-amine; 5-(6-amino-8-(6-iodo-2,3-dihydro-lH- inden-5-ylthio)-9H-purin~9-yl)-N-methylpentane~ 1 -sulfonamide; 5-(6-amino-8-(6-iodo-2,3- dihydro-1 H-inden-5-ylthio)-9H-purin-9-yI)pentane-l -sulfonamide; 1 -(3-(6-amino-8-(6-iodo- 2₅3-dihydro-lH-inden-5-yltMo)-9H-purin-9-yl)propyl)pyrrolidm-3-ol; 6-(6-amino-8-(6-iodo- 2,3-dihydro-lH-inden-5-ylthio)-9H-purin-9-yl)hexanamide; 8-((6-iodo-2,3-dihydro-lH- inden-5-yl)tiiio)-9-(2-(l-methylpiperidm-2-yl)ethyl)-9H-pmin-6-amme; 8-((6-iodo-2,3- dihydro-lH-mden-5-yl)thio)-9-(2-(l-methylpiperidin-3-yl)ethyi)-9 8-((6- iodo-2,3-dihydro- 1 H-inden-5-yl)thio)-9-(2-( 1 -(meth}^;,lsulfonyl)piperidm-3-yl)ethyl)-9H- purin-6-amine; 3-(2-(6-ammo-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)thio)-9H-purin-9- yl)ethyl)piperidine- 1 -sulfonamide; 2-fluoro-8-((6-iodo-2,3-dihydro- 1 H-inden-5-yl)methyl)-9- (2-(isobutyIamino)ethyl)-9H-purin-6-amine; 2-fluoro-8-((6-iodo-2.3-dihydro-lH-inden-5- yl)methyl)-9-(3-(isopropylamino)propyl)-9H-purin-6-amine; l-(3-(6-amino-2-fluoro-8-((6- iodo-2,3-ch¾ydro-lH-inden-5-yl)methyi)-9H-purin-9-yl)propyl)pyTrolidin^ l-(3-(3-(6- amino-2-fluoro-8-((6-iodo-2 -dihydxo-lH-indsn-5-yl)methyl)-9H-purin-9- yl)propyl)pyrrolidin-l -yl)ethanone; 9-(3-(iert-butylamino)propyl)-2-fluoro-8-((6-iodo-2,3- dihy(ko-lH-mden-5-yl)methyl)-9H-purin-6-amine; 5-(6-amino-2-fluoro-8-((6-iodo-2,3- dihydro- 1 H-inden-5-yl)methyl)-9H-purin-9-yl)-N-methylpentane- 1 -sulfonamide; 5-(6- amino-2-fluoro-8-((0-iQdo-2,3-c ^

sulfonamide; 2-fluoro-8-((6-iodo-2,3-dihydro-lH-inden-5-yl)methyl)-9-(2-(l- methylpiperidin-2--yi)eihyl)-9H-purin-6-amine; 2-fluoro-8-((6-iodo-2,3-dihydro- 1 H-inden-5- yl)methyl)-9-(2-(l -methylpiperidin-3-yl)ethyl)-9H-purin-6-amme; 2-fluoro-8-((6-iQdo-2,3- dihydro- 1 H-inden-5 -yl)methyl)-9-(2-( 1 -(methyl .sulfonyl)piperidin-3-yl)ethyl)-9H-purin-6- amine; 3-(2-(6-ammo-2-fluoro-8-((6-iodo-2,3-d ydro-l^

yl)ethyl)piperidine- 1 -sulfonamide; and 9-(3 -(tert-butylarnino)propyl)-2-fluoro-8-((6-iodo- 23-dihydro-lH-mden-5-yl)methyl)-9H-purin-6-amine

9. A compound of the formula:

wherein

(a) each of Zl , Z2 and Z3 is independently C or N, with H substituents as needed to satisfy valence:

(b) Xa and Xb are O and Xc is C¾

(c) Y is -CH₂- or -S-,

(d) X4 is hydrogen or halogen; and

(e) X₂ and R are a combination selected from:

(i) X₂ is cyano-alkyl and R is a primary amino-alkyl, a secondar>^? or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R does not include a piperidino moiety; and

(ii) X₂ is an aryl, an aikynyl, a cycloalkyl, or a cycloalkenyl group; and R is a group listed in Table 8.

10. The compound of claim 9, wherein X₂ is alkynyl.

1 1. The compound of claim 10, wherein the compound is selected from the group consisting of: 8-(6-ethynylberizo[d][l ,3]dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H- purin-6-amine; 8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9-(2-(isobutylamino)ethyl)-9H- purin-6-amine; l-(3-(2-(6-amino-8-(6-ethynylberi2o[d][l ,3]dioxol-5-ylthio)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone; 8-(6-ethynylbenzo[d] [ 1 ,3] dioxol-5 -ylthio)-9-(2-( 1 - (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine; N-(2-((2-(6-amino-8-((6- ethynylbenzo[d] [ 1 _J3]dioxol-5-yl)trno)-9H-purin-9-yl)emyl)amino)emyl)sulfamide; 3-(2-(6- arnino-8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylmio)-9H-purin-9-yl)ethylamino)-N- hydroxypropanamide; 8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yIthio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine; 8-((6-ethy ylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2- fluoro-9-(2-(isobutylamino)ethyl)-9H-purin-6-amine; 9-(3-aminopropyl)-8-(6- ethynylbenzo[d] [1 ,3]dioxol-5-ylthio)-9H-purin-6-amine; 9-(2-aminoethyl)-8-(6- ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-6-amine; 9-(3-(tert-butylamino)propyl)-8-(6- ethynylbenzo[d] [ 1 ,3]dioxol-5-yltMo)-9H-purin-6-amine; 1 -(3-(6-amino-8-(6- ethynylbenzo[d] [ 1 _i3]dioxol-5-yithio)-9H-purin-9-yi)propyl)pyrrolidin-3-one; 3-(2-(6-amino- 8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)thio)-9H-purin-9-yl)ethyl)piperidine-l-sulfonamide; 6-(6-amino-8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-9-yl)hexanamide; 1 -(6- amino-8-(6-ethynylbenzo[d] [ 1 ,3] dioxol-5 -ylthio)-9H-purin-9-yl)-3 -(tert-butylamino)propan- 2-ol; 1 -(2-((2-(6-amino-8-(6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-9- yl)ethylamino)rnethyl)pyrrolidin- 1 -yl)ethanone; 5-(6-amino-8-(6- etiiynylbenzo[d][l ,3]dioxol-5-yltiuo)-9H-purin-9-yl)pentane-l-sulfonamide; 8-(6- ethynylbenzo[d] [1 ,3]dioxol-5-ylthio)-9-(2-(l -methylpiperidin-2-yl)ethyl)-9H-purin-6-amine;

8- (6-ethynylbenzo[d] [ 1 ,3]dioxol-5-ylthio)-9-(2-(l -methylpiperidm-3-yl)ethyl)-9H-purin-6- amine; 1 -(3-(4-(6-amino-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9H-purin-

9- yl)butyl)pyrrolidin- 1 -yl)ethanone; 5-(6-amino-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5- yl)methyl)-2-fluoro-9H-purin-9-yl)pentane-l -sulfonamide; 3-(2-(6-amino-2-chloro-8-((6- ethyiiyiberiZo[d] [l ,3]dioxol-5-yl)methyl)-9H~purin-9-yl)ethyl)piperidi^ 3- (2-(6-amino-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9H-purin-9- yl)ethyl)piperidine-i -sulfonamide: 8-((6-ethynylbenzo[d][l ,3]dioxol-5-yl)methyl)-2-fluoro- 9-{2-(isob tylamino)ethy!)-9H-pu.riii-6-amine; 6-(6-animo-8-((6-eth}¾ylbeii2;o[d] [ 1 ,3]dioxol-

5- yl)methyl)-2-fluoro-9H-purin-9-yl)hexanamide; l-(3-(2-(6-amino-8-((6- ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9H-purin-9-yl)ethyl)piperidin- 1 - yl)ethanone; 8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine; 8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2- fluoro-9-(3-(isopropylamino)propyl)-9H-puriii-6-araine; 9-(3-(tert-butylamino)propyl)-8-((6- ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9H-purin-6-amine; 8-((6- ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)-2-fluoro-9-(2-(l -(methylsulfonyl)piperidin-3- yl)ethyl)-9H-purin-6-amine; 1 -(3-(6-arnmo-8-((6-ethynylberizo[d] [ 1 ,3]dioxol-5-yl)methyl)-2- fluoro-9H-purin-9-yl)propyl)pyrrolidin-3-one; 8-((6-ethynylbenzo[d][l,3]dioxol-5- yl)me1hyl)-2-fluoro-9-(2-(l-me1liylpiperidin-3-yl)et yl)-9H-purin-6-amine; l -(2-((2-(6- amino-8-((6-ethynylbenzo[d] [ 1 ,3]dioxol-5-yl)methyl)'2-fluoro-9H-piirin-9- yi)ethylamino)methyl)pyrrolidin-l-yl)ethanoiie; and 8-((6-ethynylbenzo[d][l,3]dioxol-5- yl)memyl)-2-fluoro-9-(2-(l-memylpiperid

12. The compound of claim 9, wherein X₂ is a heteroaryl group,

13. The compound of claim 12, wherein X₂ is a furan.

14. The compound of claim 13, wherein the compound is selected from the group consisting of: 8-((6-(furan-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9-(3-(isopropylamino)propyl)- 9H-purin-6-amine; 8-((6-(furan-2-yl)benzo[d][l,3]dioxol-5-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine; 5-(6-((6-amino-9-(3-(isopropylamino)propyl)- 9H-purin-8-yl)tWo)benzo[d][l ,3]dioxol-5-yl)furan-2-carbaldehyde; 9-(3- (isoprQpyiamino)propyl)-8-((6-(5-m

6- amine; 8-((6-(5-(aminomethyl)furan-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine; 8-((6-(5-memylfuran-2-yl)benzo[d] [ 1 ,3]dioxol- 5-yl)tMo)-9-(2-(neopentylammo)ethyl)-9H-purin-6-amine; 8-((6-(5-(aminomemyl)furan-2- yl)benzo[d][l,3]dioxol-5-yl)thio)-9-(2-(^^ l-(3-(2-(6-

l-yl)ethanone; .8-(6-(5-methylftLran-2-yl)berizo[d][l,3]dioxol-5-ylthio)-9-(2-(l- (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine; l-(4-(2-(6-amino-8-((6-(5- (aminomethyl)furan-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9H-purin-9-yl)ethy])piperidin- 1 - yl)ethanone; N-(2-((2-(6-amino-8-((6-(5-methylfuran-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)- 9H-purin-9-yl)ethyl)amino)ethyl) sulfamide; 3-(2-(6-amino-8-(6-(5-methylfuran-2- yl)benzo[d] [ ,3]dioxo]-5-ylthio)-9H-piirin-9-yl)ethylammo)-N-hydroxypropanamide; 9-(3- (tert-butylamino)propyl)-8-(6-(5-meth^

amine; 1 -(6-aniino-8-(6-(5-methylfuran-2-yl)benzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-9-yl)-3- (tert-butylamino)propan-2-ol; 2-(3-(6-amino-8-(6-(5-memylfuran-2-yl)benzo[d] [ 1 ,3]dioxol- 5-ylthio)-9H-purin-9-yl)propyl)aziridine-l-carbaldehyde; 5-(6-amino-8-(6-(5-methylfuran-2- yl)benzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-9-yl)pentane- 1 -sulfonamide: 1 -(3-(2-(6-am^'ino-2- fluoro-8-((6-(5-methylfuran-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone; 4-(2-(6-amino-2-fluoro-8-((6-(furan-2- yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-purin-9-yl)etliyl)piperidine- 1 -carbaldehyde; 1 -(4-(2- (6-amino-2-fluoro-8-((6-(5-methylmran-2-yl)benzo[d][l,3]dioxol-5-yl)memyl)-9H-purm yl)ethyl)piperidin- 1 -yl)ethanone; 1 -(4-(2-(6-amino-2-chloro-8-((6-(5-methylfuran-2- yl)benzo[d][l ,3]dioxol-5-yl)me yl)-9H-^ l-(4-(2-(6- arnino-8-((6-(5-(aminomemyl)furan-2-yl)benzo[d] [ 1 ]dioxol-5-yl)thio)-9H-purin-9- yl)ethyl)piperidin-l -yl)ethanone; 2-fluoro-8-((6-(furan-2-yl)benzo[d] [ 1 ,3]dioxol-5- yl)methyl)-9-(2-(isobutylamino)ethyl)-9H-purin-6-amine; 5-(6-((6-amino-2-fluoro-9-(2- (isobutylammo)ethyl)-9H-purin-8-yl)m^

2-fluoro-9-(2-(isobutylammo)ethyl)-8-((6-(5-methylfuran-2-yl)berxzo[d] [ 1 ,3]dioxol-5- yl)methyl)-9H-purin-6-amine; 8-((6-(5-(aminomemyl)furan-2-yl)benzo[d][l,3]dioxol-5- yl)memyl)-2-fluoro-9-(2-(isobutylannno)ethyl)-9H-purin-6-amine; 5-(6-amino-2-fluoro-8- ((6-(5-memylfuran-2-yl)beiizo[d][l_}3]dioxol-5-yl)memyl)-9H-purin-9-yl)pentane-l- sulfonamide; 6-(6-amino-2-fluoro-8-((6-(5-methylfuran-2-yl)benzo[d] [ 1 ,3]dioxol-5- yl)methyl)-9H-purin-9-yl)hexanamide; 2-fluoro-9-(3-(isopropylamino)propyl)-8-((6-(5- methylfuran-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-purin-6-amine; 9-(3-(tert- butylamino)propyl)-2-fluoro-8-((6-(5-memylfuran-2-yl)benzo[d][l,3]dioxol-5-yl)meth^ 9H-purin-6-amine; and 9-(3-aminopropyl)-2-fluoro-8-((6-(5-methylfuran-2- yl)benzo[d][ 1 ,3]dioxol-5-yl)methyl)-9H-purin-6-amine.

15. The compound of claim 12, wherein X₂ is an oxazole.

16, The compound of claim 15, wherein the compound is selected from the group consisting of: 8-(6-(5-methyloxazol-2-yi)benzo[d] [ 1 ,3]dioxol-5-ylthio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine; 6-(6-amino-8-(6-(5-methyloxazol-2- yl)benzo[d] [1 ,3]dioxol-5-ylthio)-9H-purin-9-yl)hexanamide; 1 -(3-(2-(6-amino-8-(6-(5- methyloxazol-2-yl)benzo[d][l,3]dioxol-5-yll o)-9H-purm-9-yl)ethyl)piperi

yi)ethanone; 9-(3-(isopropylainino)propyl)-8-((6-(oxazol-2-yl)benzo[d][l,3]ciioxol-5-yl)thio)- 9H-purin-6-amine; 9-(3-aminopropyl)-8-(6-(5-methyloxazol-2-yl)benzo[d][l ,3]dioxol-5- ylthio)-9H-purin-6-amine; 9-(3-(tert-butylamino)propyl)-8-((6-(oxazol-2- yi)benzo[d] [ 1 ,3 ]dioxol-5-yl)tMo)-9H-purin-6-amine; 1 -(4-(2-(6-amino-8-((6-(oxazol-2- yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9H-purin-9-yl)ethy3)piperidin-l -yl)ethanone; 8-((6-(5- methyioxazol-2-yl)benzo[d][l,3]dioxol-5-yl)tM^

yl)ethyi)-9H-purin-6-amine; 9-(3-(tert-butylamino)propyl)-8-((6-(5-methyloxazol-2- yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9H-purin-6-amine; 1 -(6-amino-8-((6-(5-methyloxazol-2- yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9H-purin-9-yl)-3-(isopropylamino)propan-2-ol; 5-(6- amino-8-(6-(5-methyloxazol-2-yl)benzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-9-yl)pentane- 1 - sulfonamide: 8-(6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5-ylthio)-9-(3-(l- (memylsulfonyl)pyn-olidin-3-yl)propyl)-9H-purin-6-amine; l-(3-(6-amino-8-(6-(5- methyloxazol-2-yl)benzo[d] [ 1 ,3]dioxol-5-ylthio)-9H-purin-9-yl)propyl)pyrrolidin-3-one; I - (3-(2-(6-animo-2-fluoro-8-((6-(5-methy!oxazol-2-yl)benzo[d][l ,3]dioxol-5-yl)methyl)-9H- purin-9-yl)ethyl)piperidin-l-yl)ethanone 3-(2-(6-amino-2-chloro-8-((6-(5-methyloxazol-2- yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-purin-9-yi)ethyl)piperidine- 1 -sulfonamide; 1 -(3-(2- (6-amino-8-((6-(5-(ammom.ethyl)oxazol-2-yl)benzo[d][l,3]dioxol-5-y])methyI)-2-fluoro purin-9-yl)ethyl)piperidin-l-yl)ethanone; 2-fluoro-9-(2-(isobutylamino)ethyl)-8-((6-(oxazol- 2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-purin-6-amine; 1 -(3-(6-amino-2-fluoro-8-((6-(5- methyloxazol-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-9H-purin-9-yl)propyl)pyrrolidin-3-one; 6-(6-ammo-2-fluoro-8-((6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5-yl)methyl)-9H-puri^ 9-yl)hexanamide; 5-(6-amino-2-fluoro-8-((6-(5-methyloxazol-2-yl)benzo[d][l,3]dioxol-5- yl)methyl)-9H-purin-9-yl)pentane-l -sulfonamide; 2-fiuoro-9-(3-(isopropyianimo)propyl)-8- ((6-(5-methyloxazol-2-yl)benzo[d] [ 1 ,3]dioxol-5-yl)memyl)-9H-purin-6-amine; 9-(3-(tert- buiylamino)prQpyl)-2~fluoro-8-((6~(5-^

9H-purm-6-amine; 9-(3-(tert-butylaraino)propyl)-2-fluoro-8-((6-(oxazol-2- yl)benzo[d] [ 1₅3]dioxol-5-yl)methyl)-9H-purin-6-arnine; and 9-(3-ammopropyl)-2-fluoro-8-

((6-(5-methyloxazol-2-yI)benzo[d][l,3]&^

17. The compound of claim 12, wherein X₂ is a pyrazole.

18. The compound of claim 17, wherein the compound is selected from the group consisting of: 8-((6-(lH-pyrazol-3-yl)benzo[d][l,3]dioxol-5-yl)thio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine; 8-((6-(5-methyl- 1 H-pyrazol-3- yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9-(2-(neopentylarnino)ethyl)-9H-purin-6-amine; 1 -(3-(2-(6- amino-8-(6-(5-niethyl-l H-p Tazol-3-yl)benzo[d][l,3]dioxol-5-ylt

yl)ethyl)piperidm- 1 -yl)ethanone; 8-((6-(l H-pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9-(2- (neopentylamino)ethyl)-9H-purin-6-amine; 8-(6-(l H-pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol-5- ylthio)-9-(2-(l-(methylsuliOnyl)piperidin-3-yl)ethyl)-9H-purin-6-amme 1-(3-(2-(8-(6-(1Η- pyrazol-3-yl)benzo[d] [ 1 ]dioxol-5-ylthio)-6-amino-9H-purin-9-yl)ethyl)piperidin- 1 - yl)ethanone; 1 -(3-(2-(6-arnino-8-(6-(5-methyl- 1 H-pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol-5- yltWo)-9H-purin-9-yl)ethyl)piperidin-l-yl)ethanone; 4-(2-(8-((6-(lH-pyrazol-3- yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-6-amino-9H-purin-9-yl)ethyl)piperidine- 1 -carbaldehyde; N-(2-((2-(8-((6-(lH-pyrazol-3-yl)benzo[d][1 ]dioxol-5-yl)t o)-6-ammo-9H-purin-9- yl)ethyl)amino)ethyl) suifamide; N-(2-((2-(8-((6-( 1 H-pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol-5- yl)thio)-6-amino-9H-purin-9-yl)ethyl)amino)ethyl) suifamide; 3-((2-(8-((6-(lH-pyrazol-3- yl)benzo[d] [ 1 ,3]dioxol-5-yl)tMo)-6-amino-9H-purin-9-yl)e1iiyl)amino)-N- hydroxypropanamide; 8-((6-(lH-pyrazol-3-yl)benzo[d][l,3]dioxol-5-yl)thio)-9-(3- aminopropyl)-9H-purin-6-amine; 8-((6-(l H-pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol-5-yl)thio)-9-(3- (tert-butylamino)propyl)-9H-purin-6-amine; 1 -(8-((6-(l H-pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol- 5-yl)tMo)-6-aniino-9H-purin-9-yl)~3-(isopropyiamino)propan-2-ol; 5-(8-(6-(lH-pyrazol-3- yi)benzo[d][l,3]dioxoi-5-yltb.io)-6-ammo-9H-purm-9-yl)pentane-l-sulfonamide; 6-(8-(6- ( 1 H-pyrazol-3-yi)benzo[d] [ 1 ,3]dioxol-5-ylthio)-6-amino-9H-purin-9-yl)hexanam.ide; I -(3-(8- (6-(iH-pyrazol-3-yl)benzo[d][l,3]dioxol-5-ylthio)-6-amino~9H-piirin-9~yl)pro

3-one; 8-((6-(lH-pyrazol-3-yl)benzo[d][l,3]dioxol-5-yl)methyl)-2-fluoro-9-(2- (isobutylammo)ethyl)-9H-purin-6-amine; 2-iluoro-9-(2-(isobutylamino)ethyl)-8-((6-(5- methyl H-p>Tazol-3-yl)benzo[d][l,3]dioxol-5-yl)methyl)-9H-purin-6- 8-((6-(lH- pyra^ol~3-yl)bexizo[d][l,3]dioxol-5-yl)methyl)-2-fluoro-9-(2-(l-(methy

yl)ethyi)-9H-purin-6-amine; l-(3-(2-(8-((6-(lH-pyrazol-3-yl)benzo[d][l ,3]dioxol-5- y].)methyl)-6-amino-2-fluoro-9H-puiin-9-yl)ethy].)piperidm- 1 -yl)ethanone; 4-(2-(8-((6-( 1 H- pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-6-aimno-2-cUoro-9H-purin-9- yl)ethyl)piperidine- 1 -carbaldehyde; 8-((6-(l H-pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol-5- yl)metJiyl)-9-(2-arninoethyl)-2-iluoro-9H-purin-6-ami l-(3-(8-((6-(lH-pyrazol-3- yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyl)-6-arnino-2-fluoro-9H-purin-9-yl)propyl)pyrrolid one: 5-(8-((6-(lH-pyrazol-3-yl)berizo[d][l,3]dioxol-5-yl)methyl)-6-arnino-2-flu^ purin-9-yl)pentane- 1 -sulfonamide; 6-(8-((6-(l H-pyrazol-3-yl)benzo[d] [ 1 ,3]dioxol-5- yl)methyl)-6-amino-2-fluoro-9H-purin-9-yl)hexanamide; and 8-((6-(l H-pyrazol-3- yl)benzo[d] [ 1 ,3]dioxol-5-yl)methyr)-9-(3-(tert-butylamino)propyl)-2-fluoro-9H-purin-6- amine.

19, A compound of the formula:

(a) each of Zl, Z2 and Z3 is independently C or N, with H substituents as needed to satisfy valence;

(b) Xa and Xb are O, and Xc and Xd are CH₂;

(c) Y is -CH₂-, -O- or -S-;

(d) X4 is hydrogen or halogen; and

(e) X₂ and R are a combination selected from.:

(i) X₂ is halogen or cyano and R is suitably a primary amino alkyl, a secondary or tertiary alkyl-amino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R does not include a piperidino moiety; and

(ii) X₂ is selected from the group consisting of an aryl, an alkynyl, a cycloalkyl and an cycloalkenyl; and

R is a group listed in Table 8.

20. The compound of claim 19, wherein X₂ is halogen.

2 . The compound of claim 20, wherein X₂ is iodine.

22. The compound of claim 21 , wherein the compound is selected from the group consisting of: 8-((7-iodo-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)tbio)-9-(3- (isopropylamino)propyl)-9H-purin-6-arnine; 8-((7-iodo-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)tMo)-9-(2-(isobutylamino)ethyl)-9H-purin-6-amine; 8-((7-iodo-2,3- dihydrobenzo[b][l ₅4]dioxm-6-yl)tMo ^ 9-(3- ( i H-imidazol- 1 -yl)propyl)-8-((7-iodo-2,3-dihydrobenzo[b] [ 1 ,4]dioxm-6-yl)tbio)-9H-purin-6- amine; 9-(3-aminopropyl)-8-((7-iodo-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-parin-6- amine; 9-(2-aminoethyl)-8-((7-iodo-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-purin-6- amine; 9-(3-(tert-butylamino)propyl)-8-((7-iodo-2,3-dihydroberizo[b] [ 1 ,4]dioxin-6-yl)thio)- 9H-purin-6-amine; l-(6-amino-8-((7-iodo-2,3-dihydrobenzo[b][l,4]dioxm-6-yl)thio)-9H- purin-9-yl)-3-(isopropylarnino)propan-2-ol; 5-(6-amino-8-(7-iodo-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9H-purin-9-yl)pentane- 1 -sulfonamide; 1 -(3-(6-amino- 8-(7-iodo-2,3-dihydrobenzo[b] [ 1 ,4]dioxm-6-ylthio)-9H-purin-9-yl)propyl)pyrrolidin-3-one; 6-(6-amino-8-(7-iodo-2,3-dihydrobenzo[bj[l,4]dioxin-6-yithio)-9H-purin^

l-(3-(4-(6-amino-8-(7-iodo-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)-9H-purm

yl)butyl)pyrrolidin-l-yl)ethanone; and 8-(7-iodo-2,3-dihydrobenzo[b][l,4]dioxin-6-ylthio)-9- (3-(isobutylamino)propyl)-9H-purin-6-amine.

23. The compound of claim 19, wherein X₂ is heteroaryl.

24. The compound of claim 23, wherein X₂ is pyrazole.

25. The compound of claim 24, wherein the compound is selected from the group consisting of: 8-((7-(lH-pyrazol-3-yl)-2,3-dihydrobenzo[h][l ,4]dioxin-6-yl)tbio)-9-(3- (isopropylamino)propyl)-9H-purin-6-amine; 8-((7-(lH-pyrazol-3-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(2-(neopentylammo)ethyl)-9H-purin-6-amine; I -(4- (2-(8-((7-(l H-pyrazoi-3-yl)-2,3-dihydrobenzo[b] [I _?4]dioxm-6-yi)thio)-6-amino-9H-purin-9- yl)ethyl)piperidin- 1 -yl)ethanone; 8-(7-(l H-p Tazol-3-yl)-2,3-dihydrobenjzo[b] [ 1 ,4]dioxin-6- yltMo)-9-(2-(l-(methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-am N-(2-((2-(8-((7-(lH- pyrazol-3-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxm-6-yl)thio)-6-am.mo-9H-purin-9- yl)ethyl)amino)ethyi)sulfamide; 8-((7-(l H-pyrazol-3-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)thio)-9-(3-aminopropyl)-9H-purin-6-amine; 8-((7-(lH-pyrazol-3-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxm-6-yl)thio)--9-(3-(tert--butylamino)propyl)-9H-purin-6-amine; 9-(3- (isopropylamino)propyi)-8-((7-(5-meth^

6~yl)mio)-9H-purm-6-amme; 8-((7-(5-methyl- 1 H-pyrazol-3-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxm-6-yl)tMo)-9-(2-(neopentylamino)e1hyl)-9H-purin-6-amine; 1 -(8-

((7-(lH-pyrazol-3-yl)-2,3-dihydroher^

(isopropylamino)propan-2-ol; 5-(8-(7-(l H-pyrazol-3-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- ylthio)-6-amino-9H-purin-9-yl)pentane-l -sulfonamide; 6-(8-(7-(l H-pyrazol-3-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxm-6-yltMo)-6-amino-9H-purin-9-yl)hexanamide; 1 -(3-(8-(7-(l H- pyrazol-3-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yltMo)-6-amrno-9H-purin-9- yl)propyl)pyrrolidin-3-one; 8-((7-(l H-pyrazol-3-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)methyl)-2-fluoro-9-(2-(isobutylamino)ethyl)-9H~purin-6-amme; 1 -(4-(2-(8-((7-( 1 H- pyrazol-3-yl)=23-dihydrobenzo[b^

yl)ethyl)piperidin- 1 -yl)ethanone; 1 -(3-(2-(8-((7-(l H-pyrazol-3-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-6-amino-2-fluoro-9H-purin-9-yl)ethyl)piperidin- 1 - yl)ethanone; 8-((7-(lH-pyrazoi-3-yl)-2,3-dihydroberizo[b][l,4]dioxm-6-yl)methyl)-2~fluoro- 9-(2-(I-(me1_hylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine; l-(3-(8-((7-(lH-pyrazol-3- yl)-2,3-dihydrobenzo[b] [ 1 ,4](iioxin-6-yl)methyl)-6-amino-2-fluoro-9H-purin-9- yl)propyl)pyrrolidin-3-one; 8-((7-(l H-pyrazol-3-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)metiiyl)-9-(3-(tert-butylammo)propyl)-2-fluoro-9H-purin-6-amine; 1 -(8-((7-( I H-pyrazol- 3-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxm-6-yl)methyl)-6-ammo-2-fluoro-9H-purm-9-yl)-3-(tert- butylamino)propan-2-ol; 5-(8-((7-(lH-pyrazol-3-yl)-2,3-dihydrobenzo[b][l ,4]dioxin-6- yl)methyl)-6-amino-2-fluoro-9H-purin-9-yl)pentane-l-sulfonamide; 6-(8-((7-(lH-pyrazol-3- yl)-2,3-dmydrobenzo[b][l,4]dioxm-0-yl)m

yljhexanamide; and 8-((7-(lFI-pyrazoi-3-yl)-2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)methyl)-9- (2-ammoethyl)-2-fluoro-9H-pimn-6-arnme.

26. The compound of claim 23, wherein X₂ is a furan.

27. The compound of claim 26, wherein the compound is selected from the group consisting of: 8-((7-(furan~2-yl)-2_>3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(3- (isopropyiamino)propyl)-9H-piirin-6-amine; 9-(3-(isopropylamino)propyl)-8-((7-(5- methylfuran-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-purin-6-amine; 8-((7-(5- methylfuran-2-yl)-2,3-dihydiObenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(2-(neopentylamino)ethyl)- 9H-purin-6-am.ine; 8-((7-(5-(aminomethyl)fura^

yl)thio)-9-(2--(neopentyiamino)ethyl)-9H-purin-6-amine; 8-(7-(5-methylfuran-2-yl)-2.3~ dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9-(2-( 1 -(inethylsulfonyl)piperidin-3-yl)ethyl)-9H- purin-6-amme; l-(3-(2-(6-amino-8-(7-(5-methylforan-2-yl)-2,3-dihydrobenzo[b][l_?4]dioxin- 6-ylthio)-9H-purin-9-34)ethyl)piperidin-l -yl)ethanone; 1 -(4-(2-(6-amino-8-((7-(5- methylfuran-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxm-6-yl)thio)-9H-purin-9-yl)ethyl)piperidin- 1 -yl)ethanone; 1 -(3-(2-(6-amino-8-(7-(5-(aminomemyl)fui-an-2-yl)-2,3- dihydrobenzo[l j[l,4]dioxm^ 5-(6- ammo-8-(7-(5-methylfuran-2-yl)~2,3-dm^^

yl)pentane-l -sulfonamide; 1 -(3-(6-amino-8-(7-(5-methylfuran-2-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9H-purin-9-yl)propyl)p Trolidin-3-one; 1 -(6-amino-8- ((7-(5-methylfuran-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-purin-9~yl)-3- (isopropylamino)propan-2-ol; 9-(3-aminopropyi)-8-(7-(5-methylfuran-2-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9H~purm-6-amine; N-(2-((2-(6-amino-8-((7-(furan-2- yl)-2,3-dihydrobenzo[b][l,4]dioxin-6-y^ 3- ((2-(6-amino-8-((7-(foran-2-y!)-2,3-dih^

yi)ethyl)amino)-N-hydxox\'propanamide; 9-(3-(tert-butylamino)propyl)-8-(7-(5-methylfuran- 2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9H-purin-6-amme; 6-(6-ammo~2-fluoro-8- ((7-(5-me yloxazol-2-yl)-2,3-dihydrobenzo[^^ yljhexanamide; 2-fluoro-8-((7-(5-methylfiiran-2-y] .)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-0- yl)methyl)-9-(2-(l -(methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amme; 1 -(3-(2-(6- amino-2-fluoro-8-((7-(5-mei ylforan-2-yl)-2,3-dihydrobenzo[b][l,4]di

purin-9-yl)ethyl)piperidin- 1 -yl)ethanone; 1 -(4-(2-(6-amino-2-fluoro-8-((7-(5-methylfuraii-2- yl)-2,3-dihydroberizo[b][l,4]dioxin-6-yl)methyl)-9H-purin-9-yl)ethyl)piperi

yl)ethanone; 1 -(3-(2-(6-amino-8-((7-(5-(aimnomethyl)furan-2-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9H-purin-9-yl)ethyl)piperidin- 1 - yljethanone; 2-fluoro-8-((7-( ran-2~yl)-2,3-cUhydro^

(isobutylamino)ethyl)-9H-purin-6-amine; 2-fluoro-9-(2-(isobutylamino)ethyl)-8-((7-(5- metiiylfuran-2-yl)-2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)me1hyl^ 8-((7-(5- (aminomethyl)furan-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine; l-(3-(6-amino-2-fluoro-8-((7-(5-methyloxazol-2- yl)-2,3-d yclroberizo[b][l,4]dioxin-6-yl)m 2- cMoro-8-((7-(5-memylfuran-2-yl)-2 -d^

(meihyls lfonyl)pyrrolidin-3-yl)ethyl)-9FI-piirin-6-amine; 9-(3-aminopropyl)-2-fluoro-8-((7- (5-memylfuran-2-yi)-23-dihydrobe 5-(6- amino-2-fluoro-8-((7-(5-memylftiran-2-y^

purin-9-yl)pentane-l -sulfonamide; and 6-(6-aiinno-2-fluoro-8-((7-(5-memylfuran-2-yl)-2,3- dihydrobenzo [b] [ 1 ,4] dioxin-6-yl)methyl)-9H-purin-9-yl)hexanamide.

28. The compound of claim 23, wherein X₂ is an oxazole.

29. The compound of claim 28, wherein the compound is selected from the group consisting of: l-(3-(6-amino-8-(7-(oxazol-2-yl)-2,3-dmy(kobenzo[b][l,4]dioxm-6-yltihLio)- 9H-purin-9-yl)propyl)pyrrolidin-3-one; 6-(6-amino-8-(7-(5-methyloxazoi-2-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yltnio)-9H-purin-9-yl)hexanamide; 8-(7-(5-methyloxazol-2- yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxm-6-y].tmo)-9-(2-(neopentylamino)ethyl)-9H-purin-6- amine; l-(3-(2-(6-amino-8-(7-(5-memyloxazol-2-yl)-2,3-dihydrobenzo[b][l,4]dioxin-6- ylihio)-9H-purin~9-yl)ethyl)piperidin- 1 -yl)ethanone; 1 -(4-(2-(6-amino-8-((7-(5- methyloxazol-2-yl)-2,3-ch iydrobenzo[b^

1 -yl)ethanone; 8-((7-(5-methyloxazol-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(2- (l -(methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-amine; 5-(6-amino-8-(7-(5- met yloxazol-2-yl)-2,3-dihydrobenzo[b][ 1 ,4]dioxin-6-ylthio)-9H-purin-9-yl)pentane-l - sulfonamide; N-(3-(6-amino-8-((7-(5-methyloxazol-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)thio)-9H-purin-9-y].)propyl)methanesiilfonamide; l-(2-(4-(6-amino-8-(7-(5-methyloxazol- 2-yl)-2,3 -dihydrobenzo [b] [ 1₃4]dioxin-6-ylthio)~9H-piirin~9-yl)butyl)pyrrolidin- 1 - yl)ethanone; l-(6-amino-8-((7-(5-methy3oxazol-2-yl)-2,3-dihydrobenzo[b][L4]dioxki-6- yl)tWo)-9H-purin-9-yl)-3-(isopropylamino)propan-2-ol; 9-(3-(tert-butylamino)propyl)-8-((7- (oxazol-2-yl)-2,3-dihydrobenzo[b] [ i ,4]dioxin-6-yl)tMo)-9H-purin-6-amine; 9-(3- aminopropyl)-8-((7-(oxazol-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-purin-6- amine; 8-((7-(furan-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(2- (isobutylamino)ethyl)-9H-purin-6-arnine; 9-(3-(isopropylamino)propyl)-8-((7-(oxazol-2-yl)- 2_J3-dihydrobenzo[b][ l ,4]dioxin-6-yl)thio)-9H-purin-6-amine: l-(2-(4-(6-amino-8-(7-(5- melhyloxazol-2-yl)-2,3-dihydrbben2;o[b][l ,4]dioxin-6-ylthio)-9H-puri

1 -yl)ethanone; 1 -(4-(2-(6-amino-8-((7-(5-methyloxazol-2-yl)-2,3- dihydrobeiizo[b][l ,4]dioxm-6-yl)tbio)-9H-purin-9-yl)ethyl)piperi 8-((7-(5- methyloxazol-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(2-(l - (methylsulfonyl)piperidin-3-yl)ethyl)-9H-purin-6-aniine; 2-fluoro-9-(3-

(isopropylamino)propyl)-8-((7-(oxazol-2-yl)^

9H-purin-6-amine; 2-fluoro-9-(3-(isopropylamino)prppyl)-8-((7-(5-methyloxazol-2-yl)-2,3- dihydrobenzo [b] [ 1 ,4] dioxin-6-yl)methyl)-9H-purin-6-amine; 9-(3 -(tert-butylamino)propyl)-

2-fluoro-8-((7-(oxazol-2-yl)-2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)methyl)-9H-purin-6-amm^

9-(3-(tert-butylamino)propyl)-2-fluoro-8-((7-(5-methyloxazol-2-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-9H-purin-6-amine; 6-(6-amino-2-fluoro-8-((7-(5- methyloxazol-2-yl)-2,3-dihydrobei^

5-(6-amino-2-fluoro-8-((7-(5-methyloxazol-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)methyl)~9H-p rin-9-yl)pentane-l -sulfonamide: l-(3-(6-amino-2-fl¾oro-8-((7-(5- methyloxazol-2-yl)-2,3-dihydrobenzo[b][l,4]dioxin-6-yl)inethyl)-9H-purin-9- yl)propyl)pyrrolidin-3-one; l-(3-(6-amino-2-fluoro-8-((7-(oxazol-2-yl)-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-9H-purijQ-9-yl)propyl)pyrrolidin-3-one; and 9-(3- aminopropyl)-2-fluoro-8-((7-(5-methyloxazol-2-yl)-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)methyl)-9H-purin-6-amine.

30. The compound of claim 19, wherein X₂ is alk nyl.

31. The compound of claim 30, wherein the compound is selected from the group consisting of: 8~((7-ethynyl-2,3-dihydrobenzorb][1.4]dioxin-6-yl)thio)-9-(3- (isopropylammo)propyl)-9H-purin-6-amme; 3-(3-(6-amino-8-(7-ethynyl-2.3- dihydrobenzo[bj [ 1 ,4]dioxin-6-ylthio)-9H-puiin-9-yl)propyl)p>TTolidine- 1 -carbaldehyde; 8- ((7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9-(2-(neopentylamino)ethy3)-9H- purm-6-amine: 9-(2~aminoethyl)-8-((7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)- 9H-purin-6-amine; 1 -(3-(2-(6-ammo--8-(7-ethynyl-2,3-dihydrobeiizo[b] [ 1 ,4]dioxin-6-ylthio)- 9H-purin-9-yl)ethyi)piperidm-l-yl)ethanone; 8-(7-ethynyl-2_!3-dihydrobenzo[b][l,4]dioxin-6- ylt o)-9-(2-(l"(methylsulfonyi)piperidin-3-yl)ethyl)-9H-purin-6-amine; N-(2-((2-(6-amino- 8-((7-e1hynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)thio)-9H-purm-9- yl)ethyl)amino)ethyl)sulfamide; 9-(3-aminopropyl)-8-((7-ethynyl-2,3- dihyckobeiizo[b][l,4]dioxin-6-yl)tliio)-9H-purin-6-amine; 6-(6-ainino-8-(7-ethynyl-2,3- dihydxobenzo[b] [ 1 ,4]dioxm-6-ylthio)-9H-purin-9-yl)hexanamide; 5-(6-amino-8-(7-ethynyl- 2,3-dihydrobenzo[b] [ 1 ,4] dioxin-6-ylthio)-9H-purin-9-yl)pentane-l -sulfonamide; 1 -(6-amino- 8-((7-ethynyl-2,3-dihydroberizo[b][l,4]dioxin-6-yl)thio)-9H-purin-9-y

(isopropylamino)propan-2-ol; 9-(3-(tert-butylaniino)propyl)-8-(7-eth nyl-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9H-purin-6-amine; 8-(7-ethynyl-2,3- dihydrobenzo[h][l,4]dioxm-6-yltm

8-(7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)-9-(2-(l -methylpiperidin-3-yl)ethyl)- 9H-purin-6-amine; 9-(2-aminoethyl)-8-(7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-ylthio)- 9H-purin-6-amine; 8-((7-ethynyl-2,3-dihy(kobenzo[b][l,4]iMoxin-6-yl)methyl)-2-fiuoro-9-(2- (isobutylamino)ethyl)-9H-purin-6-amine; 8-((7-ethynyl-2,3-dihydrobenzo[b][l,4]dioxin-6- yl)memyl)-2-fluoro-9-(2-(l-(methylsulfonyl)piperidin-3-yl)ethyl)-9H-puri l-(3-(2- (6-amino-8-((7-ethynyl-2,3-dihydrob^

yl)ethyl)piperidin-l-yl)ethanone; 3-(2-(6-amino-8-((7-ethynyl-2,3- dihydrobenzo[b][l ,4]dioxin-6-yl)methy^

carbaldehyde; 1 -(3-(6-amino-8-((7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2- fluoro-9H-puri.n-9-yl)propyl)pyrrolidin-3-one; 6-(6-ammo-8-((7-ethynyl-2,3- dihydrobenzo[b] [ 1 ,4]dioxm-6-yl)methyl)-2-fluoro-9H-purin-9-yl)hexanamide; 1 -(6-amino-8- ((7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9H-purin-9-yl)-3-(tert- butylamino)propan-2-ol; 5-(6-amino-8-((7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)methyl)-2-fluoro-9H-purin-9-yl)pentane-l -sulfonamide; 8-((7-ethynyl-2,3- dihydrobenzo [b] [ 1 ,4] dioxin-6-yl)methyl)-2-fluoro-9-(3 -(isopropylamino)propyl)-9H-purin-6- amine; 9-(3-(tert-butylamino)propyl)-8-((7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6- yl)methyl)-2-fluoro-9H-purin-6-amine; 9-(3-aminopropyl)-8-((7-ethynyl-2,3- dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)memyl)-2-fluoro-9H-purin-6-ainine; 8-((7-ethynyl-2,3- dihydrobenzo[b][l ,4]dioxin-6-yl)nieihyl)-2-fluoro-9-(2-(l-methylpiperi

purin-6-amine; and 8-((7-ethynyl-2,3-dihydrobenzo[b] [ 1 ,4]dioxin-6-yl)methyl)-2-fluoro-9- (2-(i~niethylpiperidin-3-yl)ethyl)-9H-purin-6-amine.

32. A compound of the formula:

wherein

(a) each of Zl , Z2 and Z3 is independently C or N, with H substituents as needed to satisfy valence;;

(b) one of Xa and Xb is O and Xc and the other of Xa and Xb are -CH₂-;

(c) Y is -CH₂- or -S-,

(d) X4 is hydrogen or halogen; and

(e) X₂ and R in combination are selected from the group consisting of:

(i) X₂ is halogen and R is a primary arnino-alkyl, a secondary or tertiary alkyl-amino-alkyl, an aryl-alkyl, or a nonaroniatic heterocycle-alkyl, with the proviso that R does not include a piperidino moiety; or

(ii) X₂ is an aryl, an alkynyl, an amino, a cycloalkyl, or a cycloalkenyl and R is a group listed in Table 8. 33. A compound of the formula:

wherein

(a) each of Zl , Z2 and Z3 is independently C or N, with H substituents as needed to satisfy valence;

(b) one of Xa and Xb is S and Xc and the other of Xa and Xb are -CH₂-;

' (c) Y is -CH₂- or -S-,

(d) X4 is hydrogen or halogen; and

(e) X₂ is selected from the group consisting of alkyl, alkenyl, alkynyl, aryl, cycloalkyl, cycloalkenyl, saturated or unsaturated heterocycle, aryl, halogen, aryloxy, alkoxy, halogenated alkoxy, alkenyloxy, hydroxyalkyl, arnino, alkylarnino, chalkylamino, acylarnino, carbamyl, amido, dialkylamido, alkylamido, alkylsulfonamido, sulfonamido, trihalocarbon, - thioalkyl, S0₂-alkyl, -COO-alkyl,- COalkyl, OH, N0₂, CN or alkyl-CN, or part of a ring formed by R, and

(f R is a group listed in Table 8.

34. A compound of the formula:

Rectified Sheet

(Rule 91) ISA7AU

wherein

(b) one of Xa or Xb is a substituted or unsubstituted N, and Xc and the other of Xa and Xb are C¾_,

(c) Y is -CH₂- or -S-;

(d) X4 is hydrogen or halogen;

(e) X₂ is selected from the group consisting of alkyl, alkenyl, alkynyi, aryl, cycioalkyl, cycloalkenyl, saturated or unsaturated heterocycle, aryl, halogen, aryloxy, alkoxy, halogenated alkoxy, alkenvloxy, hydroxyalkyl, amino, alkylamino, dialkylamino, acylammo, carbamyl, amido, dialkylamido, alkylamido, alkylsulfonamido. sulfonamide, trihalocarbon, - thioalkyl, S0₂-alkyl, -COO-alkyi,- COalky!. OH, N0₂, CN or alky!-CN, or part of a ring formed by R, and

(f) R is a group listed in Table 8,

A compound of the formula:

wherein

(b) one of Xa or Xb is carbonyl (C=0) or thiocarbonyl (C=S), and Xc and the other of Xa and Xb is CH_2;

(c) Y is -CH>- or -S-, (d) X4 is hydrogen or halogen;

(e) .2 is selected from the group consisting of alkyi, alkenyl, alkynyl, aryl, cycloalkyi, cycloalkenyl, saturated or unsaturated heterocycle, aryl, halogen, aryloxy, alkoxy, halogenated alkoxy, alkenyloxy, hydroxyalkyl, amino, alkylamino, dialkylamino, acylamino, carbamyl, amido, dialkylamido, alkylamido, alkylsulfonamido, sulfonamido, trihalocarbon, - thioalkyl, S0₂-aikyl, -COO-alkyl,- COalkyl, OH, N0₂, CN or alkyl-CN, or part of a ring formed by R, and

(f) R is a group listed in Table 8, with the proviso that wwhen X₂ or CN, R is not piperidi.no.

36. A compound of the formula:

wherein

(b) Xa, Xb and Xc include at least one non-carbon atom and -Xa-Xc-Xb- includes a double bond;

(c) Y is -CH₂- or -S-,

(d) X4 is hydrogen or halogen; and

(e) X₂ is selected from the group consisting of alkyi, alkenyl, alkynyl, aryl, cycloalkyi, cycloalkenyl, saturated or unsaturated heterocycle, aryl, halogen, aryloxy, alkoxy, halogenated alkoxy, alkenyloxy, hydroxyalkyl, amino, alkylamino, dialkylamino, acylamino, carbamyl, amido, dialkylamido, alkylamido, alkylsulfonamido, sulfonamido, trihalocarbon, - thioalkyl, S0₂-alkyl, -COO-alkyl,- COalkyl, OH, N0₂, CN and alkyl-CN, or part of a ring formed by R, and

(f) R is a group listed in Table 8. A compound of the formula:

wherein

^" (b) one or both of Xa or Xb is a heteroatom, with the proviso that both of Xa and Xb are not O

(c) Y is -CH₂-₅ -O- or -S-;

(d) X₄ is hydrogen or halogen; and

(e) X₂ and R are a combination selected from:

(i) X₂ is halogen or CN and R is a primary aminoalkyl, a secondary or tertian' alkyl-araino-alkyl, a trialkylammonioalkyl group, an aryl-alkyl, or a nonaromatic heterocycle-alkyl, with the proviso that R does not include a piperidino moiety: and

(ii) X₂ is an aryl, an aikynyl and an amino; and

R is selected from the groups listed in Table 8

38. A compound of the formula:

wherein (a) each of Zl, Z2 and Z3 is independently C or N, with H substituents as needed to satisfy valence;

(b) Xa, Xc, Xd and Xb are all carbon connected by single or double bonds.

(c) Y is -CH₂-, -O- or -S-;

(d) X₄ is hydrogen or halogen;

(e) X₂ is selected from the group consisting of alkyl, alkenyi. alkynyl, aryl, cycloalkyl, cycloalkenyl, saturated or unsaturated heterocycle, aryl, halogen, aryloxy, alkoxy, halogenated alkoxy, alkenyloxy, hydroxyalkyl, aniino, alkylamino, dialkylamino, acylamino, carbamyl, amido, dialkylamido, alkylamido, alkylsulfonamido, sulfonamido, trihalocarbon, - thioalkyl, S0₂-alkyl, -COO-alkyl,- COalkyi, OH, N0₂, CN or alkyl-CN, or part of a ring formed by ; and

(f) R is a group listed in Table 8.

A compound of the formula

wherein

(a) R₁ is alkyl;

(b) Y is S or CH₂,

(c) X4 is H or halogen,

(d) X₂ is a saturated or unsaturated non-aromatic carbocycle or heterocycle, an aryl, an alkylamino, a dialkylamino, an alkynyl or is part of a ring formed by R; and

(e) R is hydrogen, alkyl, alkenyi, or alkynyl, linear, branched or cyclic, optionally including heteroatoms such as N, S or O, optionally connected to the 2 '-position to form an 8 to 10 member ring. A compound of the formula

wherein

(a) Y is CH₂, S, O, C=0, C=S, or N;

(b) Xd is H or halogen;

(c) Xa, Xb, Xc and Xd are independently selected from C, O, N, S, carbonyl, and thionyl, connected by single or double bonds with H as needed to satisfy valence,

(d) X₂ is an alkynyl group and

(e) R is a group listed in Table 8 .

A compound of the formula

wherein

(a) YisCH₂,S,0,00,C=S,orN;

(b) X4 is H or halogen;

(d) X₂ is a furan, thiophene, pyrazole, oxazole or thiazole and

(e) R is a group listed in Table 8.

42. The compoundof any of claims 1, 2, 4, 5, 7, 9, 10, 12, 13, 15, J 7, 19-21, 23, 24, 26, 28, 30, 32-38, 40 and 41, wherein Zl, Z2, and Z3 are all nitrogen.

43. The compound of any of claims 1,2,4,5,7,9, 10, 12, 13, 15, 17, 19-21,23,24,26, 28, 30, 32-38, 40 and 41, wherein Z2 or Z3 is carbon.

44. The compound of any of claims 1,2,4,5, 7, 9, 10, 12, 13, 15, 17, 19-21,23, 24,26, 28, 30, 30-38, 40 and 41 , wherein Zl and Z3 are nitrogen and Z2 is carbon.

Rectified Sheet

(Rule91)ISA/AU

45. The compound of any of claims 1, 2, 4, 5, 7, 9, 10, 12, 13, 15,^" 17, 19-21, 23, 24, 26, 28, 30, 32-38, 40 and 41, wherein Z3 is carbon.

46. Use of a compound in accordance with any preceding claim in formulating a pharmaceutical composition for the inhibition of hsp90.

47. Use of a compound in accordance with any preceding claim in . formulating a pharmaceutical composition for the treatment of cancer or neurodegenerative disease.

48. A method of treating cancer of neurodegenerative disease comprising administering a therapeutically effective amount of a compound in accordance with any preceding claim.

49. A compound as shown in any of the tables herein.

Rectified Sheet

(Rule 91) ISA/ALT