WO2011032692A1 - Process for preparing 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h-imidazol[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process - Google Patents

Process for preparing 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h-imidazol[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process Download PDF

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WO2011032692A1
WO2011032692A1 PCT/EP2010/005668 EP2010005668W WO2011032692A1 WO 2011032692 A1 WO2011032692 A1 WO 2011032692A1 EP 2010005668 W EP2010005668 W EP 2010005668W WO 2011032692 A1 WO2011032692 A1 WO 2011032692A1
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formula
compound
bromo
propionic acid
methyl ester
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PCT/EP2010/005668
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French (fr)
Inventor
Stuart Gary Tilbrook
Andreas Schumacher
René Emmenegger
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Paion Uk Limited
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Priority to EP10760917.4A priority Critical patent/EP2477967B1/en
Priority to CN201080049975.3A priority patent/CN102753525B/en
Priority to US13/496,742 priority patent/US9156842B2/en
Priority to JP2012529154A priority patent/JP5801309B2/en
Priority to DK10760917.4T priority patent/DK2477967T3/en
Priority to RU2012115456/04A priority patent/RU2551848C2/en
Priority to PL10760917T priority patent/PL2477967T3/en
Application filed by Paion Uk Limited filed Critical Paion Uk Limited
Priority to KR1020127009891A priority patent/KR101801735B1/en
Priority to ES10760917.4T priority patent/ES2555233T3/en
Priority to MX2015018057A priority patent/MX341093B/en
Priority to MX2012003183A priority patent/MX337683B/en
Priority to BR112012006053-8A priority patent/BR112012006053B1/en
Priority to AU2010294873A priority patent/AU2010294873B2/en
Priority to CA2774018A priority patent/CA2774018C/en
Publication of WO2011032692A1 publication Critical patent/WO2011032692A1/en
Priority to HK12113326.4A priority patent/HK1173437A1/en
Priority to US14/841,899 priority patent/US9512078B2/en
Priority to HRP20151316TT priority patent/HRP20151316T1/en
Priority to US15/336,143 priority patent/US10000464B2/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/24—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D213/36—Radicals substituted by singly-bound nitrogen atoms
    • C07D213/40—Acylated substituent nitrogen atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/24—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D213/44—Radicals substituted by doubly-bound oxygen, sulfur, or nitrogen atoms, or by two such atoms singly-bound to the same carbon atom
    • C07D213/46—Oxygen atoms
    • C07D213/50—Ketonic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
    • C07F9/65586—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system at least one of the hetero rings does not contain nitrogen as ring hetero atom

Definitions

  • the present invention relates to a process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2- pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]-propionic acid methyl ester and the benzene sulfonate salt thereof, starting from 3-[(S)-7-Bromo-2-((R and/or S)-2- hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester or 3-[(S)-7-Bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H- benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester, and new compounds useful as starting material or intermediate in that process.
  • WO 00/69836 describes short-acting benzodiazepines that include a carboxylic ester moiety and are inactivated by non-specific tissue esterases. An organ-independent elimination mechanism is predicted to be characteristic of these benzodiazepines, providing a more predictable and reproducible pharmacodynamic profile.
  • the compounds are suitable for therapeutic purposes, including sedative-hypnotic, anxiolytic, muscle relaxant and anticonvulsant purposes.
  • the compounds are short- acting CNS depressants that are useful to be administered intravenously in the following clinical settings: preoperative sedation, anxiolysis, and amnestic use for perioperative events; conscious sedation during short diagnostic, operative or endoscopic procedures; as a component for the induction and maintenance of general anesthesia, prior and/or concomitant to the administration of other anaesthetic or analgesic agents; ICU sedation.
  • WO 00/69836 teaches a process for preparing the above compound of formula (F), which comprises:
  • WO 2008/007071 discloses a method of preparing the besylate salt (P) of the above compound of formula (F) by adding benzene sulfonic acid to a solution of that compound in toluene or ethyl acetate, stirring, filtering, washing with toluene or ethyl acetate and drying under vacuum. That method yields 3-[(4S)-8-Bromo-1-methyl-6-(2- pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P) which is taught to be a particularly interesting active pharmaceutical ingredient (API).
  • API active pharmaceutical ingredient
  • the objective of the invention is to find a process for preparing compound (P) and precursors thereof that does not have the above drawbacks.
  • the process of the invention thus relates to a new method for preparing the compound of formula (F) or its besylate salt (P), comprising oxidizing 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM)
  • That method allows to obtain 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) or its besylate salt (P), namely 3-[(4S)-8-Bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate, with very high chemical and chiral purities.
  • the process of the invention may include steps for preparing 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]- propionic acid methyl ester of formula (EM) starting from one of the precursors of the compound of formula (F) disclosed in WO 00/69836, namely 3-[(S)-7-bromo-2-oxo-5- pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (D) or (2-amino-5-bromo-phenyl)-pyridin-2-yl-methanone of formula (A).
  • the invention concerns a process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2- pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F)
  • the compound of formula (EM) may be 3-[(S)-7-bromo-2-((R)-2-hydroxy-propylamino)- 5-pyridin-2-yl-3H-benzo[e][1 , methyl ester of formula (E)
  • the compound of formula (EM) is a compound of formula (E).
  • the compound of the formula (EM) can be obtained by reacting in an aprotic solvent the compound of formula (E1) with (R)-1-amino-2-propanol or (S)-1-amino-2-propanol, both compounds are commercially available.
  • step (b) the compound of formula (E1 ) can be reacted with (S)-1- amino-2-propanol, yielding 3-[(S)-7-bromo-2-((S)-2-hydroxy-propylamino)-5-pyridin-2- yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula ( ⁇ ').
  • step (b) the compound of formula (E1) is reacted with (R)-1-amino-2- propanol, yielding the preferred 3-[(S)-7-bromo-2-((R)-2-hydroxy-propylamino)-5- pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (E).
  • optical purity of the compounds of formula (E) and ( ⁇ ' ) depends on the purity of the 1-amino-2-propanol used in the synthesis. For the purposes of the present invention it is preferred to obtain the compound of the formula (E) at an optical purity of ⁇ 95%, preferably ⁇ 99%, and more preferably ⁇ 99.5%, using (R)-1-amino-2-propanol.
  • the oxidizing agent is an agent apt to oxidize a secondary alcohol to a ketone without reacting with the other reactive groups of the compound of formula (EM).
  • Suitable oxidizing agents are sulfonated pyridine in dimethylsulfoxide (DMSO) in presence of a base such as diisopropylethylamine (DIEA), oxalyl chloride in DMSO in presence of a base such as triethylamine , Albright-Goldman oxidation reagent (acetic acid anhydride / DMSO), S-IBX oxidation reagent (1-Hydroxy-(1 H)- benzo-1 ,2-iodoxol-3-one-1 -oxide), a combination of hypochlorite and a catalytic amount of tetramethylpiperidine-1-oxyl (TEMPO), and a hypervalent iodine compound of formula (DM)
  • DIEA diisopropylethylamine
  • oxalyl chloride in DMSO in presence of a base such as triethylamine
  • Albright-Goldman oxidation reagent acetic
  • R1 is acyl, preferably C 2 -C 10 acyl, more preferably C 2 -C 4 acyl, such as
  • the oxidizing agent may convert the compound of formula (EM) directly to the compound of formula (F), or to the intermediate ketone of formula
  • the compound of formula (F) may be isolated by methods routinely used in the art of synthetic organic chemistry, which may e.g. include evaporating the solvent under vacuum, dissolving the residue in a suitable solvent, filtering, washing with different aqueous solutions and re-extracting the combined aqueous solutions with a suitable solvent.
  • the compound of the formula (F) at an optical purity of ⁇ 95%, preferably ⁇ 99%, more preferably > 99.5%, and most preferably > 99.9%.
  • a preferred oxidizing agent is an hypervalent iodine compound of formula (DM)
  • R1 is acyl, preferably C 2 -C 10 acyl, in particular C 2 -C 4 acyl.
  • R1 is acetyl, the compound of formula (DM) then being 1 ,1 ,1- triacetoxy-1 ,1-dihydro-1 ,2-benzoiodoxol-3(1 H)-one) (Dess-Martin periodinane).
  • the reaction is generally performed by treating the compound of formula (EM) with a stochiometric excess, usually from 1.0 to 2.0, preferably 1.2 to 1.8, particularly 1.4 to 1.6 equivalent of Dess- Martin periodinane, in an aprotic solvent such as e.g. dichloromethane, chloroform, acetonitrile, tetrahydrofuran or butanone. Particularly good results have been obtained in butanone.
  • an aprotic solvent such as e.g. dichloromethane, chloroform, acetonitrile, tetrahydrofuran or butanone.
  • the Dess-Martin periodinane is conveniently added in solid form and in portions to a solution of the compound of formula (EM) in an aprotic solvent.
  • the reaction is preferably carried out at a temperature between 30 and 45 °C.
  • the reaction product mainly contains the compound of formula (F) and there is generally no further acidic reaction performed.
  • the compound of formula (F) may be isolated by methods routinely used in the art of synthetic organic chemistry, which may e.g. include evaporating the solvent under vacuum, dissolving the residue in a suitable solvent, filtering, washing with different aqueous solutions and re-extracting the combined aqueous solutions with a suitable solvent.
  • Another interesting oxidizing agent is a combination of a catalytic amount of tetramethylpiperidine-1-oxyl (TEMPO) and hypochlorite.
  • TEMPO tetramethylpiperidine-1-oxyl
  • the compound of formula (EM) is generally treated in solution with a catalytic amount of TEMPO, e.g. 0.005 to 0.03 equivalent of TEMPO, and 1.0 to 1. 8 equivalent of hypochlorite, usually in a solvent or a solvent mixture which contains water having a pH 6.0 to 8.0, the pH being adjusted if necessary by adding additives such as e.g. hydrogen carbonate or acetic acid, and a salt such as sodium or potassium bromide.
  • a suitable solvent mixture is e.g. ethyl acetate/toluene/water or dichloromethane/water.
  • the reaction product mainly contains 3-[(S)-7-bromo-2-(2-oxo- propylamino)-5-pyridin-2-yl-3H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (FK)
  • That compound can be cyclized into the compound of formula (F) under acidic conditions.
  • Suitable acidic conditions are generally an organic acid in an organic solvent .
  • suitable acidic conditions are p-toluene sulfonic acid in chloroform or a mixture of benzene sulfonic acid in substantially less than the stochiometric amount (preferably less than a fifth of the stochiometric amount, more preferably about a tenth of the stochiometric amount) and molecular sieve MS3A in dichloromethane.
  • the compound of formula (F) may be isolated by methods routinely used in the art of synthetic organic chemistry, which may e.g. include evaporating the solvent under vacuum, dissolving the residue in a suitable solvent, filtering, washing with different aqueous solutions and re-extracting the combined aqueous solutions with a suitable solvent.
  • the invention also concerns a process for preparing the besylate salt 3-[(4S)-8-bromo- 1 -methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P) which comprises
  • step (b) treating the reaction product obtained in step (a) with benzene sulfonic in an organic solvent or an organic solvent mixture, such as to produce the compound (P).
  • the compound (P) may be isolated by methods routinely used in the art of synthetic organic chemistry, which may e.g. include crystallisation of the besylate salt.
  • the compound of the formula (P) at an optical purity of ⁇ 95%, preferably ⁇ 99%, more preferably > 99.5%, and most preferably > 99.9%.
  • the compound of formula (EM) is a compound of formula (E).
  • the oxidation agent is a combination of hypochlorite and a catalytic amount of TEMPO, whereby the compound of formula (EM) is treated in solution with 0.005 to 0.03 equivalent of TEMPO and 1.0 to 1.8 equivalent of hypochlorite, such as to give 3-[(S)-7-bromo-2-(2-oxo-propylamino)-5-pyridin-2-yl-3H- 1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (FK)
  • step (b) treating the compound of formula (FK) with benzene sulfonic acid in an organic solvent or an organic solvent mixture, such as to produce the compound (P).
  • the organic solvent or the organic solvent mixture used in step (b) is an organic solvent capable of favouring in presence of benzene sulfonic acid the cyclization of the 3-[(S)-7-bromo-2-(2-oxo-propylamino)-5-pyridin-2-yl-3H-1 ,4-benzodiazepin-3-yl]- propionic acid methyl ester of formula (FK), the formation of the benzene sulfonate salt and optionally the crystallisation of that salt.
  • Suitable organic solvent mixtures are ethyl acetate and ethanol, ethyl acetate and 2-propanol, and ethyl acetate and methanol.
  • a preferred organic solvent mixture is ethyl acetate and ethanol.
  • LDA lithium diisopropylamide
  • BMPC bis-morpholinophosphorylchloridate
  • Step (b) reacting the compound of formula (E1) with (R)-1-amino-2-propanol or (S)-1-amino- 2-propanol.
  • Step (a) is generally performed by dissolving the compound of formula (D) in an aprotic solvent and adding LDA and BMPC.
  • LDA is added prior to BMPC.
  • the skilled person would also consider adding BMPC prior to LDA as being asuitable alternative.
  • a suitable aprotic solvent is for instance tetrahydrofurane (THF), a mixture of THF with an alkane solvent (e.g. heptane) and an aralkane solvent (e.g. ethylbenzene), or an ether, e.g. diethyl ether.
  • THF tetrahydrofurane
  • alkane solvent e.g. heptane
  • an aralkane solvent e.g. ethylbenzene
  • ether e.g. diethyl ether
  • 1.0 to 1.2 in particular 1.0 to 1.1 , equivalent of LDA and at least 2.0 equivalents of BMPC are used.
  • Step (b) is generally carried out by reacting in an aprotic solvent the compound of formula (E1) with (R)-1-amino-2-propanol or (S)-1-amino-2-propanol to yield the compound of the formula (EM).
  • step (b) the compound of formula (E1) can be reacted with (S)-1- amino-2-propanol, yielding 3-[(S)-7-bromo-2-((S)-2-hydroxy-propylamino)-5-pyridin-2- yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula ( ⁇ ' ).
  • step (b) the compound of formula (E1) is reacted with (R)-1-amino-2- propanol, yielding the preferred 3-[(S)-7-bromo-2-((R)-2-hydroxy-propylamino)-5- pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (E).
  • a suitable aprotic solvent is THF, a mixture of THF with an alkane solvent (e.g. heptane) and an aralkane solvent (e.g. ethylbenzene), or an ether, e.g. ethyl ether.
  • an alkane solvent e.g. heptane
  • an aralkane solvent e.g. ethylbenzene
  • an ether e.g. ethyl ether.
  • a suitable organic solvent is for instance THF, DMF, acetonitrile or methanol.
  • a suitable base is for instance an alkaline metal hydrogen carbonate, e.g. sodium hydrogen carbonate, or a tertiary amine, e.g. triethylamine, diisopropylethylamine, N,N- dimethylaniline and pyridine.
  • alkaline metal hydrogen carbonate e.g. sodium hydrogen carbonate
  • a tertiary amine e.g. triethylamine, diisopropylethylamine, N,N- dimethylaniline and pyridine.
  • 3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (D) may be purified by recrystallisation from a secondary alcoholic solvent, e.g. isopropanol or isobutanol, preferably isopropanol, or a solvent mixture such as e.g. ethyl acetate/heptane.
  • a secondary alcoholic solvent e.g. isopropanol or isobutanol, preferably isopropanol, or a solvent mixture such as e.g. ethyl acetate/heptane.
  • the compound of formula (C) used above as starting material for preparing 3-[(S)-7- bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (D), may be prepared by cleaving the 'Boc group of the compound of formula (B)
  • the reaction may be performed by dissolving the compound of formula (B) in a polar solvent such as ethanol or methanol, adding hydrogen chloride in 1 ,4-dioxane and preferably cooling the reaction mixture.
  • a polar solvent such as ethanol or methanol
  • the compound of formula (B) used above as starting material for preparing the compound of formula (C) may be prepared by treating (2-amino-5-bromo-phenyl)- pyridin-2-yl-methanone of formula
  • a suitable organic solvent is for instance THF, DMF, dichloromethane or ethyl acetate.
  • Suitable coupling agents include those selected from the group consisting of Dicyclohexylcarbodiimide (DCC), N,N,N ' ,N ' -Tetramethyl-0-(7-azabenzotriazol-1- yl)uronium hexafluorophosphate (HATU), 2-(1 H-Benzotriazole-1-yl)-1 , 1 ,3,3- tetramethyluronium hexafluorophosphate (HBTU), 1-Ethyl-3-(3-dimethylaminopropyl)- carbodiimide (EDC), propane phosphonic acid anhydride T3P, Benzotriazol-1-yl- oxytripyrrolidinophosphonium hexafluorophosphate PyBOP, isobutylchloroformate, carbonyldiimidazole (CDI), chlorenamine, or ⁇ , ⁇ '-diisopropylcarbodiimide (DIC).
  • a base such as e.g. diisopropylethylamine (DIEA), triethylamine (TEA) or N-methylmorpholine (NMM), or another additive may be used.
  • DIEA diisopropylethylamine
  • TEA triethylamine
  • NMM N-methylmorpholine
  • a preferred coupling agent is DCC, e.g. in dichloromethane or dimethylformamide (DMF).
  • the invention also concerns a new compound useful as starting material or intermediate for performing the above defined process for preparing 3-[(4S)-8-bromo-1- methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) or 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P), which is selected from the group consisting of a) 3-[(S)-7-Bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H
  • the invention also relates to the use of the compounds of the formula (EM), (E), (E1), (FK), (C), (B) in the preparation of a compound of the formula (F) or its besylate salt (P), and also the use of the above defined process for preparing 3-[(4S)-8-bromo-1- methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F), in the preparation of 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H- imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P), whereby the 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a
  • the compound of formula (B) (1833 g, 2642 mmol) was dissolved in 2200 ml methanol at room temperature. The solution was transferred into a 10 I reactor and cooled to a temperature below 20 °C. To this solution hydrogen chloride (11200 mmol) in 2800 ml 1 ,4-dioxane was added over a period of 15 minutes while maintaining the reaction mixture at a temperature of 15 to 10°C. The mixture was stirred for 3 hours at a temperature of 15 to 10°C. The solution obtained was directly used in Example A3 below. That solution showed for the main product the following NMR data:
  • the compound of formula (E) (874 g, 1.9 mol) was dissolved in butanone (7.8 I) and warmed to 30°C.
  • Dess Martin periodinane (968 g; 2.28 mol) was added in portions. A short time after the addition of the first amounts a solid formed being the unsoluble byproducts of the reagent. The temperature of the reaction mixture had increased to 43°C upon complete addition. This temperature was maintained for 45 minutes.
  • Dess Martin periodinane (242 g; 0.58 mol) was again added. Stirring was continued for 40 minutes. Then the volatiles were widely removed in vacuo and the residue was diluted with ethyl acetate (10 I).

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  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

The invention concerns a new process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1,2-a][1,4]benzodiazepine-4-yl]-propionic acid methyl ester 3-[(4S)-8-bromo-1 -methyl-6-(2-pyridinyl)-4H-imidazo[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P) which comprises reacting 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5-pyridin-2- yl-3H-benzo[e][1,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM) with an oxidizing agent and optionally treating the reaction product under acidic conditions, such as to produce the compound of formula (F) or the compound (P), and new compounds useful as starting material or as intermediate for performing that process.

Description

"Process for preparing 3-i(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazoi1 ,2- airi ,41benzodiazepine-4-yllpropionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process"
The present invention relates to a process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2- pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]-propionic acid methyl ester and the benzene sulfonate salt thereof, starting from 3-[(S)-7-Bromo-2-((R and/or S)-2- hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester or 3-[(S)-7-Bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H- benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester, and new compounds useful as starting material or intermediate in that process.
WO 00/69836 describes short-acting benzodiazepines that include a carboxylic ester moiety and are inactivated by non-specific tissue esterases. An organ-independent elimination mechanism is predicted to be characteristic of these benzodiazepines, providing a more predictable and reproducible pharmacodynamic profile. The compounds are suitable for therapeutic purposes, including sedative-hypnotic, anxiolytic, muscle relaxant and anticonvulsant purposes. The compounds are short- acting CNS depressants that are useful to be administered intravenously in the following clinical settings: preoperative sedation, anxiolysis, and amnestic use for perioperative events; conscious sedation during short diagnostic, operative or endoscopic procedures; as a component for the induction and maintenance of general anesthesia, prior and/or concomitant to the administration of other anaesthetic or analgesic agents; ICU sedation.
One of the compounds disclosed in that document is 3-[(4S)-8-bromo-1-methyl-6-(2- pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]-propionic acid methyl ester of formula (F) below
Figure imgf000004_0001
WO 00/69836 teaches a process for preparing the above compound of formula (F), which comprises:
- (a) preparing 3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin- 3-yl]-propionic acid methyl ester of formula (D)
Figure imgf000004_0002
by reacting (2-amino-5-bromo-phenyl)-pyridin-2-yl-methanone of formula (A)
Figure imgf000004_0003
(A)
in chloroform with an alpha-Fmoc-protected-amino acid chloride (obtained by reacting FMOC-Glu(OMe)-OH and oxalylchloride in dichloromethane), treating the obtained amide with triethylamine in dichloromethane, then with acetic acid in 1 ,2- dichloroethane,
isolating the compound of formula (D), and
- (b) reacting the compound of formula (D) with a suspension of sodium hydride in THF, treating the reaction mixture with bis-morpholinophosphochloridate (BPMC) in THF, filtering the reaction mixture, reacting the filtrate with DL-1-amino-propanol, purifying the alcoholic adduct obtained, treating that purified alcoholic adduct with a mixture of DMSO and oxalyl chloride in dichloromethane, treating the reaction mixture with triethylamine, diluting with ethyl acetate, washing with aqueous solutions and concentrating to give a foam, treating that foam with a catalytic amount of p- toluenesulfonic acid, neutralizing the solution with sodium hydrogenocarbonate and isolating the compound of formula (F).
WO 2008/007071 discloses a method of preparing the besylate salt (P) of the above compound of formula (F) by adding benzene sulfonic acid to a solution of that compound in toluene or ethyl acetate, stirring, filtering, washing with toluene or ethyl acetate and drying under vacuum. That method yields 3-[(4S)-8-Bromo-1-methyl-6-(2- pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P) which is taught to be a particularly interesting active pharmaceutical ingredient (API).
The process for preparing the direct precursor of that API, namely 3-[(4S)-8-bromo-1- methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F), or that API, 3-[(4S)-8-Bromo-1-methyl-6-(2-pyridinyl)-4H- imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P), starting from the compound of formula (A) disclosed in WO 00/69836 is not satisfying for an industrial preparation, notably because of the high number of steps, the low overall yield and the insufficient optical purity of the compounds obtained at the different steps.
The objective of the invention is to find a process for preparing compound (P) and precursors thereof that does not have the above drawbacks.
That objective is attained by the invention as defined in the appended claims.
The process of the invention thus relates to a new method for preparing the compound of formula (F) or its besylate salt (P), comprising oxidizing 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM)
Figure imgf000005_0001
or in a preferred embodiment oxidizing 3-[(S)-7-bromo-2-((R)-2-hydroxy-propylamino)- 5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (E)
Figure imgf000006_0001
That method allows to obtain 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) or its besylate salt (P), namely 3-[(4S)-8-Bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate, with very high chemical and chiral purities.
The process of the invention may include steps for preparing 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]- propionic acid methyl ester of formula (EM) starting from one of the precursors of the compound of formula (F) disclosed in WO 00/69836, namely 3-[(S)-7-bromo-2-oxo-5- pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (D) or (2-amino-5-bromo-phenyl)-pyridin-2-yl-methanone of formula (A).
It then provides a process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H- imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) or 3- [(4S)-8-Bromo-1 -methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4- yl]propionic acid methyl ester benzene sulfonate (P), with very high chemical and chiral purities, which has less steps, a better reproducibility and a better overall yield with regard to the compound of formula (D) or the compound of formula (A) than the process disclosed in WO 00/69836.
The invention concerns a process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2- pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F)
Figure imgf000006_0002
which comprises reacting 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5- pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM)
Figure imgf000007_0001
with an oxidizing agent and optionally treating the reaction product under acidic conditions, such as to produce the compound of formula (F).
The compound of formula (EM) may be 3-[(S)-7-bromo-2-((R)-2-hydroxy-propylamino)- 5-pyridin-2-yl-3H-benzo[e][1 , methyl ester of formula (E)
Figure imgf000007_0002
3-[(S)-7-bromo-2-((S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin- 3-yl]-propionic acid methyl ester of formula (Ε')
Figure imgf000007_0003
or a mixture of the compounds of formula (E) and (Ε').
Preferably the compound of formula (EM) is a compound of formula (E).
The compound of the formula (EM) can be obtained by reacting in an aprotic solvent the compound of formula (E1) with (R)-1-amino-2-propanol or (S)-1-amino-2-propanol, both compounds are commercially available.
In one embodiment in step (b) the compound of formula (E1 ) can be reacted with (S)-1- amino-2-propanol, yielding 3-[(S)-7-bromo-2-((S)-2-hydroxy-propylamino)-5-pyridin-2- yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (Ε'). Preferably in step (b) the compound of formula (E1) is reacted with (R)-1-amino-2- propanol, yielding the preferred 3-[(S)-7-bromo-2-((R)-2-hydroxy-propylamino)-5- pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (E).
The optical purity of the compounds of formula (E) and (Ε') depends on the purity of the 1-amino-2-propanol used in the synthesis. For the purposes of the present invention it is preferred to obtain the compound of the formula (E) at an optical purity of ≥ 95%, preferably≥ 99%, and more preferably≥ 99.5%, using (R)-1-amino-2-propanol.
The oxidizing agent is an agent apt to oxidize a secondary alcohol to a ketone without reacting with the other reactive groups of the compound of formula (EM).
Examples of suitable oxidizing agents are sulfonated pyridine in dimethylsulfoxide (DMSO) in presence of a base such as diisopropylethylamine (DIEA), oxalyl chloride in DMSO in presence of a base such as triethylamine , Albright-Goldman oxidation reagent (acetic acid anhydride / DMSO), S-IBX oxidation reagent (1-Hydroxy-(1 H)- benzo-1 ,2-iodoxol-3-one-1 -oxide), a combination of hypochlorite and a catalytic amount of tetramethylpiperidine-1-oxyl (TEMPO), and a hypervalent iodine compound of formula (DM)
Figure imgf000008_0001
wherein R1 is acyl, preferably C2-C10 acyl, more preferably C2-C4 acyl, such as
1 ,1 ,1 -triacetoxy-1 , 1 -dihydro-1 ,2-benziodoxol-3(1 H)-one (Dess-Martin periodinane).
The oxidizing agent may convert the compound of formula (EM) directly to the compound of formula (F), or to the intermediate ketone of formula
Figure imgf000008_0002
),
which can then be cyclized under acidic conditions into the compound of formula (F). The compound of formula (F) may be isolated by methods routinely used in the art of synthetic organic chemistry, which may e.g. include evaporating the solvent under vacuum, dissolving the residue in a suitable solvent, filtering, washing with different aqueous solutions and re-extracting the combined aqueous solutions with a suitable solvent.
For the purposes of the present invention it is preferred to obtain the compound of the formula (F) at an optical purity of≥ 95%, preferably≥ 99%, more preferably > 99.5%, and most preferably > 99.9%.
A preferred oxidizing agent is an hypervalent iodine compound of formula (DM)
Figure imgf000009_0001
(DM),
wherein R1 is acyl, preferably C2-C10 acyl, in particular C2-C4 acyl.
Most preferably R1 is acetyl, the compound of formula (DM) then being 1 ,1 ,1- triacetoxy-1 ,1-dihydro-1 ,2-benzoiodoxol-3(1 H)-one) (Dess-Martin periodinane).
Where the oxidizing agent is Dess-Martin periodinane, the reaction is generally performed by treating the compound of formula (EM) with a stochiometric excess, usually from 1.0 to 2.0, preferably 1.2 to 1.8, particularly 1.4 to 1.6 equivalent of Dess- Martin periodinane, in an aprotic solvent such as e.g. dichloromethane, chloroform, acetonitrile, tetrahydrofuran or butanone. Particularly good results have been obtained in butanone.
The Dess-Martin periodinane is conveniently added in solid form and in portions to a solution of the compound of formula (EM) in an aprotic solvent.
Where the aprotic solvent is butanone, the reaction is preferably carried out at a temperature between 30 and 45 °C.
Where the oxidizing agent is Dess-Martin periodinane, the reaction product mainly contains the compound of formula (F) and there is generally no further acidic reaction performed.
The compound of formula (F) may be isolated by methods routinely used in the art of synthetic organic chemistry, which may e.g. include evaporating the solvent under vacuum, dissolving the residue in a suitable solvent, filtering, washing with different aqueous solutions and re-extracting the combined aqueous solutions with a suitable solvent.
Another interesting oxidizing agent is a combination of a catalytic amount of tetramethylpiperidine-1-oxyl (TEMPO) and hypochlorite.
The compound of formula (EM) is generally treated in solution with a catalytic amount of TEMPO, e.g. 0.005 to 0.03 equivalent of TEMPO, and 1.0 to 1. 8 equivalent of hypochlorite, usually in a solvent or a solvent mixture which contains water having a pH 6.0 to 8.0, the pH being adjusted if necessary by adding additives such as e.g. hydrogen carbonate or acetic acid, and a salt such as sodium or potassium bromide. A suitable solvent mixture is e.g. ethyl acetate/toluene/water or dichloromethane/water.
Where the oxidizing agent is a combination of a catalytic amount of TEMPO and hypochlorite, the reaction product mainly contains 3-[(S)-7-bromo-2-(2-oxo- propylamino)-5-pyridin-2-yl-3H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (FK)
Figure imgf000010_0001
That compound can be cyclized into the compound of formula (F) under acidic conditions.
Suitable acidic conditions are generally an organic acid in an organic solvent .
Examples of suitable acidic conditions are p-toluene sulfonic acid in chloroform or a mixture of benzene sulfonic acid in substantially less than the stochiometric amount (preferably less than a fifth of the stochiometric amount, more preferably about a tenth of the stochiometric amount) and molecular sieve MS3A in dichloromethane.
The compound of formula (F) may be isolated by methods routinely used in the art of synthetic organic chemistry, which may e.g. include evaporating the solvent under vacuum, dissolving the residue in a suitable solvent, filtering, washing with different aqueous solutions and re-extracting the combined aqueous solutions with a suitable solvent. The invention also concerns a process for preparing the besylate salt 3-[(4S)-8-bromo- 1 -methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P) which comprises
(a) reacting 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H- benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM)
Figure imgf000011_0001
(EM) with an oxidizing agent, and
(b) treating the reaction product obtained in step (a) with benzene sulfonic in an organic solvent or an organic solvent mixture, such as to produce the compound (P).
The compound (P) may be isolated by methods routinely used in the art of synthetic organic chemistry, which may e.g. include crystallisation of the besylate salt.
For the purposes of the present invention it is preferred to obtain the compound of the formula (P) at an optical purity of≥ 95%, preferably≥ 99%, more preferably > 99.5%, and most preferably > 99.9%.
Preferably the compound of formula (EM) is a compound of formula (E).
In a preferred embodiment the oxidation agent is a combination of hypochlorite and a catalytic amount of TEMPO, whereby the compound of formula (EM) is treated in solution with 0.005 to 0.03 equivalent of TEMPO and 1.0 to 1.8 equivalent of hypochlorite, such as to give 3-[(S)-7-bromo-2-(2-oxo-propylamino)-5-pyridin-2-yl-3H- 1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (FK)
Figure imgf000011_0002
(FK), and
(b) treating the compound of formula (FK) with benzene sulfonic acid in an organic solvent or an organic solvent mixture, such as to produce the compound (P). The organic solvent or the organic solvent mixture used in step (b) is an organic solvent capable of favouring in presence of benzene sulfonic acid the cyclization of the 3-[(S)-7-bromo-2-(2-oxo-propylamino)-5-pyridin-2-yl-3H-1 ,4-benzodiazepin-3-yl]- propionic acid methyl ester of formula (FK), the formation of the benzene sulfonate salt and optionally the crystallisation of that salt.
Examples of suitable organic solvent mixtures are ethyl acetate and ethanol, ethyl acetate and 2-propanol, and ethyl acetate and methanol.
A preferred organic solvent mixture is ethyl acetate and ethanol.
3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H- benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM) used as starting material in the above defined process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2- pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) or 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P), may be prepared by a method comprising
(a) reacting 3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin-3- yl]-propionic acid methyl ester
Figure imgf000012_0001
with lithium diisopropylamide (LDA) and bis-morpholinophosphorylchloridate (BMPC), such as to give the compound of formula (E1),
Figure imgf000012_0002
(E1 ), and
(b) reacting the compound of formula (E1) with (R)-1-amino-2-propanol or (S)-1-amino- 2-propanol. Step (a) is generally performed by dissolving the compound of formula (D) in an aprotic solvent and adding LDA and BMPC. Usually LDA is added prior to BMPC. The skilled person would also consider adding BMPC prior to LDA as being asuitable alternative.
A suitable aprotic solvent is for instance tetrahydrofurane (THF), a mixture of THF with an alkane solvent (e.g. heptane) and an aralkane solvent (e.g. ethylbenzene), or an ether, e.g. diethyl ether.
Generally 1.0 to 1.5 equivalent of LDA and at least 1.5 equivalents of BMPC are used.
Preferably 1.0 to 1.2, in particular 1.0 to 1.1 , equivalent of LDA and at least 2.0 equivalents of BMPC are used.
Step (b) is generally carried out by reacting in an aprotic solvent the compound of formula (E1) with (R)-1-amino-2-propanol or (S)-1-amino-2-propanol to yield the compound of the formula (EM).
In one embodiment in step (b) the compound of formula (E1) can be reacted with (S)-1- amino-2-propanol, yielding 3-[(S)-7-bromo-2-((S)-2-hydroxy-propylamino)-5-pyridin-2- yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (Ε').
Preferably in step (b) the compound of formula (E1) is reacted with (R)-1-amino-2- propanol, yielding the preferred 3-[(S)-7-bromo-2-((R)-2-hydroxy-propylamino)-5- pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (E).
An example of a suitable aprotic solvent is THF, a mixture of THF with an alkane solvent (e.g. heptane) and an aralkane solvent (e.g. ethylbenzene), or an ether, e.g. ethyl ether.
3-[(S)-7-bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin- 3-yl]-propionic acid methyl ester of formula (EM) is usually isolated and purified by recrystallisation from a suitable solvent, preferably one selected from the group of ethyl acetate, isobutylacetate, 2-propanol, toluene, or ethyl acetate/heptane.
3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (D) used above as starting material for preparing 3-[(S)-7- bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]- propionic acid methyl ester of formula (EM), may be prepared by a method comprising cyclizing the compound of formula (C)
Figure imgf000014_0001
by treatment with a base in an organic solvent or organic solvent mixture.
A suitable organic solvent is for instance THF, DMF, acetonitrile or methanol.
A suitable base is for instance an alkaline metal hydrogen carbonate, e.g. sodium hydrogen carbonate, or a tertiary amine, e.g. triethylamine, diisopropylethylamine, N,N- dimethylaniline and pyridine.
3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (D) may be purified by recrystallisation from a secondary alcoholic solvent, e.g. isopropanol or isobutanol, preferably isopropanol, or a solvent mixture such as e.g. ethyl acetate/heptane.
The compound of formula (C) used above as starting material for preparing 3-[(S)-7- bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (D), may be prepared by cleaving the 'Boc group of the compound of formula (B)
Figure imgf000014_0002
by treatment with hydrogen chloride.
The reaction may be performed by dissolving the compound of formula (B) in a polar solvent such as ethanol or methanol, adding hydrogen chloride in 1 ,4-dioxane and preferably cooling the reaction mixture. The compound of formula (B) used above as starting material for preparing the compound of formula (C) may be prepared by treating (2-amino-5-bromo-phenyl)- pyridin-2-yl-methanone of formula
Figure imgf000015_0001
with 'Boc-GluiOMeJ-OH in an organic solvent in presence of a coupling agent.
A suitable organic solvent is for instance THF, DMF, dichloromethane or ethyl acetate.
Suitable coupling agents include those selected from the group consisting of Dicyclohexylcarbodiimide (DCC), N,N,N',N'-Tetramethyl-0-(7-azabenzotriazol-1- yl)uronium hexafluorophosphate (HATU), 2-(1 H-Benzotriazole-1-yl)-1 , 1 ,3,3- tetramethyluronium hexafluorophosphate (HBTU), 1-Ethyl-3-(3-dimethylaminopropyl)- carbodiimide (EDC), propane phosphonic acid anhydride T3P, Benzotriazol-1-yl- oxytripyrrolidinophosphonium hexafluorophosphate PyBOP, isobutylchloroformate, carbonyldiimidazole (CDI), chlorenamine, or Ν,Ν'-diisopropylcarbodiimide (DIC).
Depending on the coupling agent, a base such as e.g. diisopropylethylamine (DIEA), triethylamine (TEA) or N-methylmorpholine (NMM), or another additive may be used.
A preferred coupling agent is DCC, e.g. in dichloromethane or dimethylformamide (DMF).
The invention also concerns a new compound useful as starting material or intermediate for performing the above defined process for preparing 3-[(4S)-8-bromo-1- methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) or 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P), which is selected from the group consisting of a) 3-[(S)-7-Bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H
benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (E)
Figure imgf000016_0001
b) 3-[(S)-7-Bromo-2-(2-oxo-propylamino)-5-pyridin-2-yl-3H-1 ,4-benzodiazepin-3-yl]- propionic acid methyl ester of formula (FK)
Figure imgf000016_0002
(FK), c) the compound of formula (E1)
Figure imgf000016_0003
e) the compound of formula (B)
Figure imgf000017_0001
The invention also relates to the use of the compounds of the formula (EM), (E), (E1), (FK), (C), (B) in the preparation of a compound of the formula (F) or its besylate salt (P), and also the use of the above defined process for preparing 3-[(4S)-8-bromo-1- methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F), in the preparation of 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H- imidazo[1 ,2-a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P), whereby the 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) is treated with benzene sulfonic acid in a solvent or a solvent mixture, preferably selected from the group consisting of ethanol, 2-propanol, ethanol/ethyl acetate, 2-propanol/ethyl acetate and methanol/ethyl acetate. The formed besylate salt (P) is then optionally crystallized from that solvent or solvent mixture.
The following examples illustrate the invention.
Example A1 :
Preparation of the compound of formula (B)
Figure imgf000017_0002
222g (801 mmol) (2-amino-5-bromo-phenyl)-pyridin-2-yl-methanone of formula (A)
Figure imgf000017_0003
(A) (prepared as described in European Journal of Organic Chemistry, 2006, 13, 2987- 2990) and 230g (881 mmol) 'Boc-Glu DMeJ-OH were mixed as solids and dissolved in 1200 ml dichloromethane and the solution was cooled to a temperature of - 10°C. A solution of coupling reagent dicyclohexylcarbodiimide DCC (165 g, 801 mmol) in 400 ml dichloromethane was added dropwise over a period of 1 hour while the internal temperature was kept at a temperature from - 10°C to - 5°C, then the solution was stirred for 40 hours at a temperature of - 5°C to 0 °C. The suspension was filtered, the filter cake was washed with 1000 ml dichloromethane and the filtrate was evaporated to a yellow residue, showing for the main product the following NMR data:
1 H-NMR (CDCI3, 300 MHz) 11.29 (brs, 1 H); 8.65(dt, 1 H, J= 4.8, 1.4); 8.50 (d, 1 H, J= 9.0); 7.91(d, 1 H, J= 2.3); 7.86 (m, 2H); 7.59 (dd, 1 H, J= 9.0, 2.3) 7.45 (ddd, 1 H, J= 6.4, 4.0, 2.3); 5.27 (brd, 1 H, J=6.4); 4.27 (brm, 1 H); 3.60 (s, 3H); 2.52-2.14 (m, 2H); 2.04-1.82 (m, 2H); 1.36 (s, 9H), corresponding to the compound of formula (B).
Example A2:
Preparation of the compound of formula (C)
Figure imgf000018_0001
The compound of formula (B) (1833 g, 2642 mmol) was dissolved in 2200 ml methanol at room temperature. The solution was transferred into a 10 I reactor and cooled to a temperature below 20 °C. To this solution hydrogen chloride (11200 mmol) in 2800 ml 1 ,4-dioxane was added over a period of 15 minutes while maintaining the reaction mixture at a temperature of 15 to 10°C. The mixture was stirred for 3 hours at a temperature of 15 to 10°C. The solution obtained was directly used in Example A3 below. That solution showed for the main product the following NMR data:
1 H-NMR (CDCI3, 300 MHz) 1 .23 (brs, 1 H); 8.63(dt, 1 H, J= 4.6, 1.3); 8.36 (brd, 2H, J= 4.3); 8.06 (d, 1 H, J= 1.3); 8.0 (m, 1 H); 7.78 (dd, 1 H, J= 8.6, 2.4) 7.64 (m, 2H) 7.46 (d, 1 H, 8.6); 4.00 (brm, 1 H); 3.62 (s, 3H); 2.41-2.23 (m, 2H); 1.83-1.56 (m, 2H), corresponding to the compound of formula (C).
Example A3:
Preparation of 3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin- 3-yl]-propionic acid methyl ester of formula (D)
Figure imgf000019_0001
449 g (5343 mmol) bf sodium hydrogen carbonate were suspended in 2000 ml of acetonitrile under vigorous stirring. The solution of the compound of formula (C) obtained in Example A2 above (1743 g, 763 mmol) was added to that suspension in 4 equal portions at room temperature over a period of 30 minutes (actual solvent ratio methanol/1 ,4-dioxane/acetonitrile: 3/4/10). The temperature decreased to 15 °C, with an intensive gas development and a slight foaming after addition of each portion. The colour switched after each addition from orange (colour of the hydrochloric solution) to yellow-green. The yellow-green solution was stirred at a temperature of about 15 °C for 3 hours 40 minutes. The reaction mixture was filtered over a thin layer of celite, washed with acetonitrile and evaporated in vacuo at 50 °C bath temperature, yielding 424 g of viscous resin, The resin was dissolved in 1500 ml of 2-propanol at 85 °C. After cooling the precipitated solid was isolated by filtration and washed with 2-propanol and dried at a temperature of 35 °C in vacuo to give the product as a yellow crystalline solid (215.8g, 528mmol) having the following NMR data:
1 H-NMR (CDCI3, 300 MHz) 8.69 (s, 1 H); 8.52 (dq, 1 H, J= 4.8, 1.6, 0.8); 7.99 (dt, 1 H, J= 8.0, 1.0); 7.73 (td, 1 H J= 7.8, 1.8);7.54-7.43(m, 2H) 7.28 (qd, 1 H, J= 7.6, 4.8, 1.0); 6.93(d, 1 H, J=8.6); 3.67 (dd, 1 H J= 7.6, 6.1); 3.60 (s, 3H) 2.66-2.34 (m, 4H), corresponding to the compound of formula (D).
For the process described in Examples A1 to A3, the overall yield from (2-amino-5- bromo-phenyl)-pyridin-2-yl-methanone of formula (A) to 3-[(S)-7-bromo-2-oxo-5-pyridin- 2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin-3-yl]-propionic acid methyl ester of formula (D) is about 67 %. The chemical purity of the isolated solid as determined by HPLC (at 230nm) was 98.35% and its optical (chiral) purity dedtermined by HPLC (at 290 nm) was 100%.
Example A4:
Preparation of 3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4-benzodiazepin- 3-yl]-propionic acid methyl ester of formula (E)
Figure imgf000019_0002
1.36 kg (3.39 mol) compound of formula (D) was suspended in 3500 ml dry tetrahydrofurane (THF) under argon and cooled down to -18 °C. A 2M solution of lithium diisopropylamide LDA (3.4 mol) in 1700 ml THF/heptane/ethylbenzene was added over a period of 90 minutes. The addition was exothermic and the temperature was controlled to be between -10 and -5 °C. The mixture was then stirred for 105 minutes at 0°C, followed by the portionwise addition of bis-morpholinophosphoryl- chloridate BMPC (1.74 kg; 6.78 mol) over 15 minutes. The brown suspension was stirred for 150 minutes and the temperature held between -5 and Q°C An analytical amount of the main product isolated by chromatography was found to have the following NMR data:
1 H-NMR (CDCI3, 300 MHz) 8.68 (ddd, 1 H, J= 4.8, 1.7, 0.9); 7.96 (dt, 1 H, J= 7.9, 1.0); 7.8 (td, 1 H, J= 7.7, 1.8); 7.67 (dd, 1 H, J= 8.6, 2.3); 7.57 (d, 1 H, J= 2.0); 7.41 (ddd, 1 H, J= 7.5, 4.8, 1.2); 7.36 (d, 1 H, J= 8.6); 7.2 (m, 1H); 3.85- 3.65 (m, 8H + 3H ) 3.33-3.19 (m, 8H); 2.76-2.45 (m, 4H), corresponding to the compound of formula (E1 )
Figure imgf000020_0001
(E1 ).
A solution of (R)-1-amino-2-propanol (519 g; 6.91 mol) in dry THF (1500 ml) was added within 105 minutes and the temprature was kept between +4 and -2°C. The mixture was stirred for 16 hours at room temperature. A second addition of the (R)-1 - amino-2-propanol (102 g; 1.36 mol) in dry THF (150 ml) was carried out within 5 minutes. It was stirred for another 25 hours, then evaporated widely.
To the pasty residue were added dichloromethane (5 I) and saturated aqueous sodium bicarbonate solution (5 I). The layers were separated. The organic layer was washed with saturated aqueous ammonium chloride solution (2.5 I) and water (2 I). Each aqueous layer was re-extracted with dichloromethane (200 ml). The combined organic layers were dried over sodium sulfate, filtered and evaporated. The crude product was recrystallised from 95°C hot toluene after slow cooling and filtration as a yellowish solid in 56 % yield (876 g) with the following NMR data:
1 H-NMR (CDCI3, 300MHz) 8,6 (ddd, 1 H, J=4.8, 1.6, 1.0); 7.87 (dm, 1 H, J=7.8); 7.79 (td, 1 H, J= 7.5, 1.7); 7.51 (dd, 1 H, J=8.7, 2.3); 7.39 (d, 1 H, J=2.3); 7.36 (ddd, 1 H, J= 7.4, 2.5, 1.4); 7.13 (d, 1 H, J=8.8); 5.76 (tb, 1 H) 5.19 (b, 1 H); 3.98 (m, 1 H) 3.71 (s, 3H); 3.5-3.2 (m, 3H) 2.9-2.3 (m, 4H); 1.17 (d, 3H, J= 6.3), corresponding to the compound of formula (E). Chemical purity as determined by HPLC (254nm) was 98.77%. The optical purity as determined by HPLC (290nm) was 99.54%.
Example A5:
Preparation of 3-[(4S)-8-bromo-1 -methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) using Dess- Martin periodinane as oxidizin
Figure imgf000021_0001
The compound of formula (E) (874 g, 1.9 mol) was dissolved in butanone (7.8 I) and warmed to 30°C. Dess Martin periodinane (968 g; 2.28 mol) was added in portions. A short time after the addition of the first amounts a solid formed being the unsoluble byproducts of the reagent. The temperature of the reaction mixture had increased to 43°C upon complete addition. This temperature was maintained for 45 minutes. Dess Martin periodinane (242 g; 0.58 mol) was again added. Stirring was continued for 40 minutes. Then the volatiles were widely removed in vacuo and the residue was diluted with ethyl acetate (10 I). The solids were removed by filtration and washed with ethyl acetate (0.5 I). The combined filtrates of both runs were washed with saturated aqueous sodium bicarbonate solution (7 I) and saturated aqueous ammonium chloride solution (7 I). The combined aqueous layers were re-extracted with ethyl acetate (0.5 I). The combined organic layers were extracted three times with 1 N hydorochloric acid (4 1, 2 x 2 I). The combined aqueous layers was washed with ethyl acetate (100 ml). Ethyl acetate (7 I) was added to the aqueous layer, followed by the slow addition of 1 N sodium hydroxide solution (8 I) under vigorous stirring. At a pH between 4 to 5 the product went into the organic layer as indicated by a color switch of the layers. After complete addition, the pH value was 9. The layers were separated and the aqueous extracted with ethyl acetate (1 I). The combined organic layers were washed with water dried over sodium sulfate, filtered and evaporated. The crude product was obtained as a brown resin (783 g, chemical purity by HPLC at 230 nm = 93.91 %, chiral purity by HPLC (250nm) is = 98.47%) still containing 13 wt % of ethyl acetate, which has the following NMR data: 1 H-NMR (CDCI3, 300 MHz) 8.58 (ddd, 1 H, J= 4.8, 1.7, 0.9); 8.19 (dt, 1 H, J= 7.9, 1.0); 7.8 (td, 1 H, J= 7.7, 1.8); 7.72 (dd, 1 H, J= 8.6, 2.3); 7.66 (d, 1 H, J= 2.3); 7.34 (ddd, 1 H, J= 7.6, 4.8, 1.2); 7.31 (d, 1 H, J= 8.6); 6.87 (dm, 1 H, j=1.0) 4.09-4.02 (m, 1 H); 3.68 (s, 3H); 2.9-2.7 (m, 4H); 2.35 (d 3H, J= 1.0), which correspond to the compound of formula (F). The calculated molecular weight was 640 g/mol corresponding to a yield 76 %. Example A6:
Preparation of 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F) using a combination of a catalytic amount TEMPO and hypochlorite as oxidizing agent followed by acidic cyclisation a) Formation of 3-[(S)-7-bromo-2-(2-oxo-propylamino)-5-pyridin-2-yl-3H-1 ,4- benzodiazepin-3-yl]-propionic acid methyl ester of formula (FK) in ethyl acetate/toluene
Figure imgf000022_0001
230 mg (0.5 mmol ) of the compound of formula (E) were dissolved in 1 ml of dichloromethane and cooled to less than 0°C (in an ice/ethanol bath) giving a yellowish solution. 4.2 mg of sodium hydrogen carbonate (0.05 mmol) and 2.6 mg sodium bromide (0.025 mmol) were added. 0.78 mg (0.005 mmol) of TEMPO was added, immediately followed by 0.33 ml of a 2.1 mol/l NaOCI solution (0.7 mmol). The organic layer was colorless and the aqueous layer yellowish. The reaction mixture was stirred vigorously overnight. HPLC showed as main product a ketone and no presence of the compound of formula (E). The isolated main product showed the following NMR data: 1 H-NMR (CDCI3, 300 MHz) 8.65 (ddd, 1 H, J= 4.8, 1.8, 0.8); 7.93 (dt, 1 H, J= 7.9, 1.0); 7.8 (td, 1 H, J= 7.7, 1.8); 7.52(dd, 1 H, J= 8.6, 2.3); 7.41 (d, 1 H, J= 2.3); 7.36 (ddd, 1 H, J= 7.5, 4.8, 1.2); 7.2 (d, 1 H, J= 8.8); 4.25 (dd, 2H, J= 90, 20); 3.71 (s, 3H) 3.36 (m, 1 H); 2.85-2.40 (m, 4H); 2.21 (s, 3H), corresponding to the compound of formula (FK). b) Cyclisation into the compound of formula (F)
1.83 g (4 mmol) of the compound of formula (FK) was dissolved in 15 ml chloroform. 76 mg (0.4 mmol) p-toluene sulfonic acid was added and the mixture was refluxed for 3 days. The reaction mixture was diluted with dichloromethane washed with sodium hydrogen carbonate solution, dried with magnesium sulphate and evaporated. The isolated main product showed the following NMR data:
1 H-NMR (CDCI3, 300 MHz) 8.58 (ddd, 1 H, J= 4.8, 1.7, 0.9); 8.19 (dt, 1 H, J= 7.9, 1.0); 7.8 (td, 1 H, J= 7.7, 1.8); 7.72 (dd, 1 H, J= 8.6, 2.3); 7.66 (d, 1 H, J= 2.3); 7.34 (ddd, 1H, J= 7.6, 4.8, 1.2); 7.31 (d, 1 H, J= 8.6); 6.87 (dm, 1 H, j=1.0) 4.09-4.02 (m, 1 H); 3.68 (s, 3H); 2.9-2.7 (m, 4H); 2.35 (d 3H, J= 1.0), corresponding to the compound of formula (F).
Example A7:
Preparation of 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P) by one- pot cyclization of the compound of formula (FK) and salt formation
2g (3.63 mmol) of the compound of formula (FK) were dissolved in 12 ml ethyl acetate at room temperature. 0.563 g (3.56 mmol) of benzene sulfonic acid was dissolved in 5.6 ml ethanol and added drop wise over 5 minutes to the reaction mixture. Precipitation. After 1 hour the solid was isolated by filtration, washed with ethyl acetate to give 1.09 g off-white solid, with the following NMR data:
1 H-NMR (CDCI3, 300 MHz) 8.60 (ddd, 1 H, J= 4.8, 1.7, 0.9); 8.20 (dt, 1 H, J= 7.9, 1.0); 7.9 (m, 2H); 7.8 (m, 2H); 7.53 (d, 1 H, J= 1.2); 7.47 (d, 1 H, J= 8.8); 7.44-7.36 (m, 4H); 4.46-4.39(m, 1 H); 3.62 (s, 3H); 3.0-2.6 (m, 4H); 2.43 (s, 3H, J= 1.0), corresponding to the compound (P). The chemical purity as determined by HPLC (230nm) was 99.07% and the optical purity as determined by HPLC (290 nm) was 99.98%.
Example A8:
Preparation of 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P) from 3- [(4S)-8-bromo-1 -methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2-a][1 ,4]benzodiazepine-4- yl]propionic acid methyl ester of formula (F)
The crude compound of formula (F) (783 g residue from Example A5) was dissolved in ethyl acetate (3.8 I). A solution of benzenesulfonic acid (228 g, 1.44 mol) in ethanol (1.8 I) was added within 50 minutes under stirring. The resulting sticky yellow suspension was stirred for another 50 minutes. The solid was isolated by filtration, washed with ethyl acetate (0.6 I), then dried overnight at 45°C and 125 mbar in a drying oven. Yield was 702 g (81 %) of a white solid with a chemical purity as determined by HPLC (230nm) was 99.35% and the optical purity as determined by HPLC (250nm) was 99.91%, with the following NMR data:
1 H-NMR (CDCI3, 300 MHz) 8.60 (ddd, 1 H, J= 4.8, 1.7, 0.9); 8.20 (dt, 1 H, J= 7.9, 1.0); 7.9 (m, 2H); 7.8 (m, 2H); 7.53 (d, 1 H, J= 1.2); 7.47 (d, 1 H, J= 8.8); 7.44-7.36 (m, 4H); 4.46-4.39(m, 1 H); 3.62 (s, 3H); 3.0-2.6 (m, 4H); 2.43 (s, 3H, J= 1.0), corresponding to the compound (P).

Claims

Claims
1. Process for preparing 3-[(4S)-8-brorno-1-rnethyl-6-(2-pyridinyl)-4H-imidazo[1 l2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester of formula (F)
Figure imgf000024_0001
(F)
which comprises reacting 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5- pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM)
Figure imgf000024_0002
with an oxidizing agent which is a hypervalent iodine compound of formula (DM)
Figure imgf000024_0003
wherein R1 is acyl, such as 1 ,1 ,1-triacetoxy-1 ,1-dihydro-1 ,2-benzoiodoxol-3(1 H)-one (Dess-Martin periodinane).
2. Process of claim 1 , characterized in that the compound of formula (EM) is 3-[(S)-7- bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]- propionic acid methyl ester of formula (E)
Figure imgf000024_0004
(E).
3. Process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1 ,2- a][1 ,4]benzodiazepine-4-yl]propionic acid methyl ester benzene sulfonate (P) which comprises
(a) reacting 3-[(S)-7-bromo-2-((R or S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H- benzo[e][1 ,4]diazepin-3-yl]-p rmula (EM)
Figure imgf000025_0001
with an oxidizing agent which is a hypervalent iodine compound of formula (DM)
Figure imgf000025_0002
wherein R1 is acyl, such as 1 ,1 ,1-triacetoxy-1 ,1-dihydro-1 ,2-benzoiodoxol-3(1 H)-one) (Dess-Martin periodinane), and
(b) treating the reaction product obtained in step (a) with benzene sulfonic acid in an organic solvent or an organic solvent mixture, such as to produce the compound (P), and optionally (c) isolating the compound (P) by methods routinely used in synthetic organic chemistry.
4. Process of claim 3, characterized in that the compound of formula (EM) is 3-[(S)-7- bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]- propionic acid methyl ester of formula (E)
Figure imgf000025_0003
5. Process of any of the preceding claims, characterized in that it further comprises preparing 3-[(S)-7-bromo-2-((R and/or S)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H- benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM) by a method comprising reacting a compound of formula (E1)
Figure imgf000026_0001
with (R)-1-amino-2-propanol or (S)-1-amino-2-propanol.
6. Process of claim 5, characterized in that the compound of formula (E1 ) is reacted with (R)-1-amino-2-propanol.
7. Process of claim 5 or 6, characterized in that 3-[(S)-7-bromo-2-((R and/or S)-2- hydroxy-propylamino)-5-pyridin-2-yl-3H-benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (EM) is crystallised from a solvent or a solvent mixture, such as ethyl acetate, isobutylacetate, 2- propanol, toluene or ethyl acetate/heptane.
8. Process of any of the preceeding claims, characterized in that it further comprises preparing a compound of the formula (E1) as defined in claim 10 by a method comprising reacting 3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4- benzodiazepin-3-yl]-propionic (D)
Figure imgf000026_0002
with lithium diisopropylamide (LDA) and bis-morpholinophosphorylchloridate (BMPC).
9. Process of any of the preceeding claims, characterized in that it further comprises the step of preparing 3-[(S)-7-bromo-2-oxo-5-pyridin-2-yl-2,3-dihydro-1 H-1 ,4- benzodiazepin-3-yl]-propionic acid methyl ester of formula (D) by a method comprising cyclizing the compound of formula (C)
Figure imgf000027_0001
by the treatment with a base in an organic solvent or organic solvent mixture.
10. Process of any of the preceeding claims, characterized in that it further comprises the step of preparing the compound of formula (C) by cleaving the lBoc group from compound of formula (B)
Figure imgf000027_0002
by treatment with hydrogen chloride.
11. Process of any of the preceeding claims, characterized in that it further comprises the step of preparing the compound of formula (B) by treating (2-amino-5-bromo- phenyl)-pyridin-2-yl-methanone of formula (A)
Figure imgf000027_0003
with lBoc-Glu(OMe)-OH in presence of a coupling agent.
12. A compound selected from the group consisting of a) 3-[(S)-7-Bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H- benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (E)
Figure imgf000028_0001
b) the compound of formula (C)
Figure imgf000028_0002
c) the compound of formula
Figure imgf000028_0003
13. Use of any of the compounds a) 3-[(S)-7-Bromo-2-((R)-2-hydroxy-propylamino)-5-pyridin-2-yl-3H- benzo[e][1 ,4]diazepin-3-yl]-propionic acid methyl ester of formula (E)
Figure imgf000028_0004
b) the compound of formula (C)
Figure imgf000029_0001
c) the compound of formula
Figure imgf000029_0002
for preparing a compound of the formula (F) as defined in claim 1 or a compound (P) as defined in claim 3.
PCT/EP2010/005668 2009-09-18 2010-09-15 Process for preparing 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h-imidazol[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process WO2011032692A1 (en)

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AU2010294873A AU2010294873B2 (en) 2009-09-18 2010-09-15 Process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazol[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process
US13/496,742 US9156842B2 (en) 2009-09-18 2010-09-15 Process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1,2-A][1,4]benzodiazepine-4-YL]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process
JP2012529154A JP5801309B2 (en) 2009-09-18 2010-09-15 3-[(4S) -8-bromo-1-methyl-6- (2-pyridinyl) -4H-imidazo [1,2-a] [1,4] benzodiazepin-4-yl] -propionic acid methyl ester or Method for preparing the benzenesulfonate and compounds useful in the method
DK10760917.4T DK2477967T3 (en) 2009-09-18 2010-09-15 PROCESS FOR THE PREPARATION OF 3 - [(4S) -8-bromo-1-methyl-6- (2-pyridinyl) - 4H-imidazo [1,2-a] [1,4] benzodiazepin-4-yl] propionic acid methyl ester OR benzenesulfonate THEREOF AND USEFUL LINKS IN THIS PROCESS
RU2012115456/04A RU2551848C2 (en) 2009-09-18 2010-09-15 Method of obtaining methyl ether of 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h-imidazo[1,2-a][1,4]benzodiazepin-4-yl]propionic acid or its benzosulphonate salt and compounds, applied therein
PL10760917T PL2477967T3 (en) 2009-09-18 2010-09-15 Process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4 H-imidazo[1,2-a][1,4]benzodiazepine-4-yl] propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process
MX2015018057A MX341093B (en) 2009-09-18 2010-09-15 Process for preparing 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h -imidazol[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process.
KR1020127009891A KR101801735B1 (en) 2009-09-18 2010-09-15 PROCESS FOR PREPARING 3-[(4S)-8-BROMO-1-METHYL-6-(2-PYRIDINYL)-4H-IMIDAZOL[1,2-a][1,4]BENZODIAZEPINE-4-YL]PROPIONIC ACID METHYL ESTER OR THE BENZENE SULFONATE SALT THEREOF AND COMPOUNDS USEFUL IN THAT PROCESS
ES10760917.4T ES2555233T3 (en) 2009-09-18 2010-09-15 Process for preparing methyl ester of 3 - [(4S) -8-bromo-1-methyl-6- (2-pyridinyl) -4H-imidazo [1,2-a] [1,4] benzodiazepine-4-yl ] propionic or the benzene sulfate salt thereof, and compounds useful in that process
EP10760917.4A EP2477967B1 (en) 2009-09-18 2010-09-15 Process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4 H-imidazo[1,2-a][1,4]benzodiazepine-4-yl] propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process
MX2012003183A MX337683B (en) 2009-09-18 2010-09-15 Process for preparing 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h -imidazol[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process.
BR112012006053-8A BR112012006053B1 (en) 2009-09-18 2010-09-15 PROCESS FOR PREPARATION OF 3-[(4S)-8-BROMO-METHYL-6-(2-PYRIDINYL)-4HIMIDAZO[1,2-A][1,4]BENZODIAZEPIN-4-YL]PROPIONIC ACID, COMPOUNDS AND USE OF THEM
CN201080049975.3A CN102753525B (en) 2009-09-18 2010-09-15 Preparation 3-[the bromo-1-methyl of (4S)-8--6-(2-pyridyl)-4H-imidazo [1,2-a] [Isosorbide-5-Nitrae] benzene phenodiazine *-4-base] propanoic acid methyl ester or the method for its benzene sulfonate and the compound for the method
CA2774018A CA2774018C (en) 2009-09-18 2010-09-15 Process for preparing 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h-imidazo[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process
HK12113326.4A HK1173437A1 (en) 2009-09-18 2012-12-24 Process for preparing 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h-imidazo[1,2-a][1,4]benzodiazepi ne-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process 3-[(4s)-8--1--6-(2-)-4h-[12-a][14]-4-]
US14/841,899 US9512078B2 (en) 2009-09-18 2015-09-01 Process for preparing 3-[(45)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1,2-A][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process
HRP20151316TT HRP20151316T1 (en) 2009-09-18 2015-12-03 Process for preparing 3-[(4s)-8-bromo-1-methyl-6-(2-pyridinyl)-4h-imidazol[1,2-a][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process
US15/336,143 US10000464B2 (en) 2009-09-18 2016-10-27 Process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1,2-A][1,4]benzodiazepine-4-yl]propionic acid methyl ester or the benzene sulfonate salt thereof, and compounds useful in that process

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US10342800B2 (en) 2010-11-08 2019-07-09 Paion Uk Ltd. Dosing regimen for sedation with CNS 7056 (Remimazolam)
US10052334B2 (en) 2010-11-08 2018-08-21 Paion Uk Ltd. Dosing regimen for sedation with CNS 7056 (remimazolam)
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US9827251B1 (en) 2010-11-08 2017-11-28 Paion Uk Ltd. Dosing regimen for sedation with CNS 7056 (remimazolam)
US9561236B2 (en) 2010-11-08 2017-02-07 Paion Uk Ltd. Dosing regimen for sedation with CNS 7056 (Remimazolam)
US10195210B2 (en) 2010-11-08 2019-02-05 Paion Uk Ltd. Dosing regimen for sedation with CNS 7056 (Remimazolam)
WO2013029431A1 (en) * 2011-08-31 2013-03-07 江苏恒瑞医药股份有限公司 Benzodiazepine derivatives tosylate salts, their polymorphic forms, preparation methods and uses thereof
WO2014034890A1 (en) 2012-08-31 2014-03-06 小野薬品工業株式会社 Method for administering hypnotic/sedative agent
WO2014136730A1 (en) 2013-03-04 2014-09-12 小野薬品工業株式会社 Oxidation reaction with excellent conversion rate
JPWO2014136730A1 (en) * 2013-03-04 2017-02-09 パイオン ユーケー リミティド Oxidation reaction with excellent conversion rate
US9981941B2 (en) 2013-03-04 2018-05-29 Paion Uk Limited Process for preparing 3-[(4S)-8-bromo-1-methyl-6-(2-pyridinyl)-4H-imidazo[1,2-a][1,4]benzodiazepin-4-yl]propionic acid methyl ester benzenesulfonate
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WO2017178663A1 (en) 2016-04-14 2017-10-19 Paion Uk Limited Orally inhaled and nasal benzodiazepines
WO2019020790A1 (en) 2017-07-28 2019-01-31 Moehs Iberica S.L. Method for preparing 3-[(3s)-7-bromo-2-oxo-5-(pyridin-2-yl)-2,3-dihydro-1h-[1,4]-benzodiazepin-3-yl] propionic acid methyl ester, and compounds useful in that method
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WO2019072944A1 (en) 2017-10-13 2019-04-18 Moehs Iberica, S.L. Method for preparing 3-[(4s)-8-bromo-1-methyl-6-(pyridin-2-yl)-4h-imidazo[1,2-a][1,4]benzodiazepin-4-yl]-propionic acid methyl ester, and compounds useful in said method
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US11325911B2 (en) 2017-10-13 2022-05-10 Moehs Iberica, S.L. Method for preparing 3-[(4S)-8-bromo-1-methyl-6-(pyridin-2-yl)-4H-imidazo[ 1,2-a][1,4]benzodiazepin-4-yl]-propionic acid methyl ester, and compounds useful in said method
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