WO2011012716A1

WO2011012716A1 - Oligonucleotides inhibiting cellular migration

Info

Publication number: WO2011012716A1
Application number: PCT/EP2010/061156
Authority: WO
Inventors: Florence Cabon; Virginie Firlej; Catherine Gallou-Kabani; Natalia Prevarskaya
Original assignee: Centre National De La Recherche Scientifique
Priority date: 2009-07-31
Filing date: 2010-07-30
Publication date: 2011-02-03
Also published as: JP5779178B2; DK2459230T3; CA2769538A1; US20120195890A1; ES2525255T3; EP2459230A1; JP2013500709A; PL2459230T3; PT2459230E; EP2283870A1; US8765705B2; CA2769538C; EP2459230B1

Abstract

The present invention relates to oligonucleotides inhibiting cellular migration. The present invention also relates to the use of at least one inhibitor of protein expression, which inhibits the expression of TSP1 protein, or a protein, which controls the expression of TSP1 or mediates the activity of TSP1, or one inhibitor of protein activity, said inhibitor inhibiting the activity of the TSP1 protein, in particular the activity responsible for the stimulation of cell migration, or one protein which controls the expression or mediates the activity of TSP for the manufacture of a drug for the prevention or the treatment of primary tumors or invasive or metastatic tumors.

Description

OLIGONUCLEOTIDES INHIBITING CELLULAR MIGRATION

The present invention relates to oligonucleotides inhibiting cellular migration, invasion or metastasis.

In particular, it relates to the use of double-stranded oligonucleotides for the manufacture of drug for the prevention or treatment of human pathologies, such as tumors.

More particularly, the invention aims at inhibiting the expression of genes, the products of which participate in triggering or maintaining pathological states.

The invention also relates to the pharmaceutical compositions for the inhibition of the expression of genes, the products of which participate in triggering or maintaining pathological states.

Thrombospondin -1 (TSPl) is a large -450 kDa- trimeric calcium-binding molecule composed of several domains (Carlson et al., 2008) which binds to numerous ligands and receptors, including several integrins and the scavenger receptor CD36. TSPl, which was the first antiangiogenic molecule characterized (Good et al., 1990), inhibits In vitro the migration and induces the apoptosis of endothelial cells (Jimenez et al., 2000). TSPl expression is inhibited in a large number of tumors (Ren et al., 2006; Zhang and Lawler, 2007) including primary breast tumors and androgen-dependent prostate tumors, where there is an inverse correlation between TSPl expression and the blood vessel density (MVD) (Colombel et al., 2005; Fontana et al., 2005b).. It has been previously reported that the TSPl expression is high, and no longer associated with a reduced MVD, both in breast metastases (Fontana et al., 2005a), and in invasive or metastatic prostate tumors that became refractory to hormonal ablation (Colombel et al., 2005). However, a functional involvement of TSPl in tumor development has not been established.

It is now well established that calcium, which binds to the type III repeats of TSPl and modifies its folding and properties (Adams, 2004; Carlson et al., 2008), regulates the proliferation, differentiation and apoptosis of cancer cells (Abeele et al., 2002; Lehen'Kyi et al., 2007; Thebault et al., 2006). Indeed, the cyclic morphological and adherence changes observed during cell migration are accompanied by repetitive changes in [Ca²⁺]! depending on Ca²⁺ influx through channels located on the plasma membrane channels. The molecular nature of these channels in migrating cells, and even more so for metastatic cancer cells, is still largely unknown.

RNA interference (RNAi) is a post-transcriptional gene-silencing mechanism where the introduction of double-stranded RNA into a cell inhibits gene expression in a sequence- dependent fashion. RNAi has been observed in a number of organisms such as mammals, drosophila, nematodes, fungi and plants, RNAi can be triggered in mammalian cells, notably by the introduction of synthetic siRNA (Carthew and Sontheimer, 2009)

One of the aims of the invention is to provide inhibitors of molecules which facilitate primary tumor development, or tumor invasion, or metastasis.

Another aim of the present invention is to provide inhibitors of the molecules which control or mediate the activity of the above mentioned molecules.

Another aim of the present invention is to provide pharmaceutical compositions comprising said inhibitors.

Another aim of the present invention is to provide pharmaceutical compositions which are efficient to inhibit both primary tumors and invasive or metastatic tumor progression.

The invention relies on the unexpected experimental results according to which the TSPl expression stimulates the migration of the tumor cells out of the hypoxic environment and thus its inhibition produces antitumor effects.

Thus, in a general embodiment, the invention relates to the use of at least:

- one inhibitor of protein expression, which inhibits the expression of:

- TSPl protein, or

- a protein, which controls the expression of TSPl or mediates the activity of TSPl,

or,

- one inhibitor of protein activity, said inhibitor inhibiting the activity of:

- the TSPl protein, in particular the activity responsible for the stimulation of cell migration, or

- one protein which controls the expression or mediates the activity of TSPl, for the manufacture of a drug for the prevention or the treatment of primary tumors, or invasive or metastatic tumors.

Tumors depend on an adequate blood supply for their growth. Therefore, for the man skilled in the art, inhibition of angiogenesis is a method to inhibit tumor development. A number of antiangiogenic compounds have been developed in the past to reach this goal. On the contrary, inhibition of an inhibitor of angiogenesis such as TSPl should increase blood vessel density, and is thus expected to foster tumor development. Up to now, it could not be encompassed for the man skilled in the art to inhibit the expression or the activity of TSPl protein to inhibit tumor development. The inventors have demonstrated here that although inhibition of TSPl in tumors does increase blood vessels density, TSPl inhibition results in a strong antitumoral effect.

As tumors expand, hypoxic regions frequently occur in tumors. Hypoxia stimulates the production of angiogenic factors such as Vascular Endothelial Growth Factor (VEGF), which is a strong trigger of angiogenesis. New blood vessels then irrigate the tumor and provide oxygen, thus reducing hypoxia. As hypoxia reduces, VEGF production is no longer stimulated, producing a feed back mechanism.

No such feed back mechanism occurs to control TSPl production in tumors: the inventors have shown here that hypoxia induces TSPl which inhibits angiogenesis, and thus increases hypoxia.

The Inventors have also demonstrated here that TSPl can be induced by a second mechanism: pharmacologic molecules such as camphor or thapsigargin that increase the cytosolic calcium concentration induce TSPl secretion and/or production. Such an increase in calcium concentration can be produced by a deregulation of calcium channels. The inventors demonstrate that several calcium channels of the Transient Receptor Potential (TRP) family regulate TSPl expression. Of note, several of these channels were shown to be upregulated in cancer cells (Prevarskaya et al, 2007).

Inductions of TSPl by hypoxia or by a modification of intracellular calcium concentration are two processes that can occur independently. However, some cross talks may occur between these processes as the inventors show that at least one of TRP channels,

TRPV3, is upregulated by hypoxia. In addition, the inventors show that hypoxia induces an increase in the intracellular calcium concentration.

Importantly, the Inventors have also demonstrated that in prostate tumors, TSPl is a major stimulator of cell migration in vitro and of tumor development in vivo as its silencing strongly inhibited tumor development.

An inhibitor of protein expression refers to a biological molecule, such as an oligonucleotide or a peptide, or a protein, or any other kind of organic or inorganic molecules, which inhibit the transcription of the gene coding said protein, or the translation of the mRNA of the gene coding said protein.

A protein which controls the expression of TSPl refers to a protein which controls the transcription of TSPl gene or the translation of TSPl gene transcript. For instance, several non- voltage dependent cationic channels of the Transient Receptor Potential (TRP) family, such as TRPV2, TRPV3, TRPV6, TRPM8, have been taken into account in the present invention.

A protein which mediates the activity of TSPl refers to a protein, such as the receptors of TSPl protein, which could mediate the biological activity of TSPl protein. For instance, the receptors, CD36 and B3 integrin have been taken into account in the present invention.

An inhibitor of protein activity refers to a biological molecule, such as an oligonucleotide, or a peptide, or a protein, or any other kind of organic or inorganic molecules which inhibit the biological activity of said protein in in vivo or in vitro tests.

The stimulation of cell migration refers to a situation where the percentage of migrating cells in the treated group is statistically higher than that of the control group.

The cell migration capacity can be measured in vitro using a modified Boyden Chamber. Boyden Chamber consists of two compartments separated by a permeable membrane. Cells are placed in the upper chamber and the chemotactic factors in the lower chamber. Cells migrating through the membrane are counted.

A detailed migration assay is given in the example section.

A primary tumor refers to a tumor growing at the anatomical site where tumor progression began and proceeded to yield a cancerous mass.

An invasive tumor refers to a cancer that has broken through its initial limits limiting membrane, such as the capsule of the prostate. This cancer spreads outside the tissue where it initially develops and grows into nearby, healthy tissues. "Invasive" does not imply that the cancer has already spread outside the prostate. "Invasive" has the same meaning as infiltrating.

A metastatic tumor refers to a cancer that has spread from its original site to one or more additional body sites.

In another embodiment, the inhibitor of protein expression inhibits the expression of proteins chosen from the group comprising TSPl, TRPV2, TRPV3, TRPV6, TRPM8, CD36, or B3 integrin.

TRP V2, TRPV3, TRPV6, TRPM8 belong to non-voltage dependent cationic channels of the Transient Receptor Potential (TRP) family. These proteins control the expression of TSPl protein.

CD36 and B3 integrin are receptors of TSPl. It is known that TSPl binds to a number of receptors (Roberts, 2008). These receptors can mediate the activity of TSPl protein. In another embodiment, the inhibitor of protein expression inhibits the in vivo and in vitro expression of proteins chosen from the group comprising TSPl, TRPV2, TRPV3, CD36, or B3 integrin.

In vivo tests involve living animals, including human, mice and rat. In the present invention, the in vivo inhibition of protein expression can be measured by the growth and the volume of tumoral tissue which is xenografted into nude mice. The reduction of the volume of a tumoral tissue after the injection of the inhibitor of protein expression, or the inhibitor of protein activity, means the in vivo inhibition of said protein expression.

In vitro tests refer, for instance, to the use of cells in culture, or real-time RT-PCT, etc., in order to measure the inhibition of protein expression.

In another embodiment, the inhibitor of protein expression inhibits the in vivo expression of proteins chosen from the group comprising TSPl, TRPV2, TRPV3.

In another embodiment, the inhibitor of the protein expression is a double-stranded oligonucleotide or a single-stranded oligonucleotide.

By the expression "oligonucleotide" is meant a polynucleotide from 2 to 100, and more particularly from 5 to 50, and preferably 13 to 25 nucleotides, and in particular 19, 20, 21 oligonucleotides, of type of ribonucleotides, deoxyribonucleotides or the mix of them.

A double-stranded oligonucleotide can refer to a siRNA. A single-stranded oligonucleotide can refer to a microRNA or any anti-sense single strand oligonucleotide used to inhibit the expression of target gene.

The use of a double-stranded oligonucleotide is more efficient than other previous anti-RNA strategies such as ribozyme or single stranded antisense deoxynucleotides because it cleaves repeatedly its target mRNA.

Furthermore, a double-stranded oligonucleotide is more stable than a single-stranded oligonucleotide.

In another embodiment, the inhibitor of the protein expression is:

- a double-stranded oligonucleotide comprising two oligonucleotide sequences, (a) and (b), forming a hybrid,

wherein the oligonucleotide sequence (a)

- is either complementary to the oligonucleotide sequence (b),

- or presents less than 40% mismatches with said oligonucleotide sequence (b), and

wherein the oligonucleotide sequence (a) - is either complementary to a target sequence belonging to the RNA or DNA molecule coding one of the proteins above-defined, the expression of which is to be inhibited,

- or presents less than 40% mismatches with a target sequence belonging to the RNA or DNA molecule coding one of the proteins above- defined, the expression of which is to be inhibited,

- or a fragment of the above-defined double-stranded oligonucleotides (a) and (b), comprising two complementary fragments of the respective above-defined oligonucleotide sequences (a) and (b), provided that said fragment conserves the property of inhibiting the expression of one of the proteins above-defined.

The oligonucleotide sequence (a) is preferably complementary to the oligonucleotide sequence (b), but can comprise 1-8 mismatches, particularly 5, more particularly 3 mismatches, still more particularly 1 mismatch with the oligonucleotide sequence (b).

The oligonucleotide sequence (a) is preferably complementary to the target sequence, but can comprise 1-8 mismatches, particularly 5, more particularly 3 mismatches, still more particularly 1 mismatch with the target sequence; this application is particular when the length of the target sequence is of 21 nucleotides.

In another embodiment, the inhibitor of the protein expression is:

- a double-stranded oligonucleotide comprising two oligonucleotide sequences, (a) and (b), forming a hybrid, wherein each oligonucleotide sequence comprises at one of its 3' or 5' ends, one to five unpaired nucleotides forming single- stranded ends extending beyond the hybrid, wherein the part inside the hybrid of the oligonucleotide sequence (a)

- is either complementary to the oligonucleotide sequence (b),

wherein the oligonucleotide sequence (a)

- is either complementary to a target sequence belonging to the RNA, or DNA molecule coding one of the proteins above-defined, the expression of which is to be inhibited,

- or presents less than 40% mismatches with a target sequence belonging to the RNA or DNA molecule coding one of the proteins above- defined, the expression of which is to be inhibited, - or a fragment of the above-defined double-stranded oligonucleotides (a) and (b), comprising two complementary fragments of the respective above-defined oligonucleotide sequences (a) and (b), provided that said fragment conserves the property of inhibiting the expression of one of the proteins above-defined.

In an advantageous embodiment, the oligonucleotide sequence complementary to the target sequence comprises from 15 to 25 nucleotides.

The oligonucleotide sequence complementary to the target sequence is an antisense strand, and wherein the second oligonucleotide sequence complementary to the first sequence is a sense strand.

The nature of the nucleotides comprised in the oligonucleotide sequence of the present invention is ribonucleotide, deoxyribonucleotide or both of them.

The nucleotides comprised in the oligonucleotide sequence of the present invention can be natural nucleotides (A, T, G, C, U), or chemically modified nucleotides, or a mix of them, in particular chemically modified nucleotides comprising a reactive group, or a linking agent, such as 5-methylcytidine, xanthinosine pseudouridine, dihydrouridine, inosine, ribothymidine, 7-methylguanosine or Locked Nucleic Acids (LNA).

Preferably, the oligonucleotide sequence complementary to the target sequence, also designed by the antisense strand, comprises substantially natural ribonucleotides and the sense strand, can comprise ribonucleotides, deoxyribonucleotides or both of them.

The above mentioned definition also applies to the pharmaceutical compositions and the oligonucleotides sequences further described.

In an advantageous embodiment, the double-stranded oligonucleotide comprises, at the 3' end of each of the two said oligonucleotide sequences, 1 to 5 nucleotides, preferably 2 to 3 nucleotides, extending beyond the hybrid.

In a more advantageous embodiment, the nucleotides extending beyond the hybrid are deoxythymidines. In a particular embodiment, the expression of the target sequence represented by SEQ ID NO: 41 (TSPl) is inhibited by the double-stranded oligonucleotide described in the present invention.

In another particular embodiment, the expression of the target sequence represented by SEQ ID NO: 42 (TRPV3) is inhibited by the double-stranded oligonucleotide described in the present invention.

In another particular embodiment, the double-stranded oligonucleotide is chosen from one of the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO:

3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 9 and SEQ ID NO: 10) ; (SEQ ID NO: 11 and SEQ ID NO. 12); (SEQ

ID NO: 13 and SEQ ID NO: 14); (SEQ ID NO: 15 and SEQ ID NO: 16); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 19 and SEQ ID NO: 20); (SEQ ID NO: 21 and SEQ ID

NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26);

(SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 29 and SEQ ID NO: 30); (SEQ ID NO: 31 and SEQ ID NO: 32); (SEQ ID NO: 33 and SEQ ID NO: 34); (SEQ ID NO: 35 and SEQ

ID NO: 36); (SEQ ID NO: 37 and SEQ ID NO: 38); (SEQ ID NO: 39 and SEQ ID NO: 40).

Table 1 gives the list of the double-stranded oligonucleotide sequences used in the present invention. The gene targeted in humans by each corresponding double-stranded oligonucleotide sequence is given in the first column of the table. For TSPl , TRPV3, TRPM8, 4 different couples of oligonucleotides are shown, and two for CD36, integrin B3, TRPV2,

TRP V6.

Target of the

oligonucleotide With dTdT Without dT dT

TSPl SEQ ID NO 1 CCUUGACAACAACGUGGUGdTdT SEQ ID NO 21 CCUUGACAACAACGUGGUG

Sequence TSPIa, cross

species SEQ ID NO 2 CACCACGUUGUUGUCAAGGdTdT SEQ ID NO 22 CACCACGUUGUUGUCAAGG

TSPl SEQ ID NO 3 UACCCGAGACGAUUGUAUGdTdT SEQ ID NO 23 UACCCGAGACGAUUGUAUG

Sequence TSPIb SEQ ID NO 4 CAUACAAUCGUCUCGGGUAdTdT SEQ ID NO 24 CAUACAAUCGUCUCGGGUA

TRPV3 SEQ ID NO 5 CAAGGAGAGCGAACGCAUCdTdT SEQ ID NO 25 CAAGGAGAGCGAACGCAUC

Sequence TRPV3a SEQ ID NO 6 GAUGCGUUCGCUCUCCUUGdTdT SEQ ID NO 26 GAUGCGUUCGCUCUCCUUG

TRPV3 SEQ ID NO 7 AUGUACAGCGUCAUGAUCCdTdT SEQ ID NO 27 AUGUACAGCGUCAUGAUCC

Sequence TRPV3b, cross

species SEQ ID NO 8 GGAUCAUGACGCUGUACAUdTdT SEQ ID NO 28 GGAUCAUGACGCUGUACAU

TRPM8 SEQ ID NO 9 UCUCUGAGCGCACUAUUCAdTdT SEQ ID NO 29 UCUCUGAGCGCACUAUUCA

Sequence TRPM8a SEQ ID NO 10 UGAAUAGUGCGCUCAGAGAdTdT SEQ ID NO 30 UGAAUAGUGCGCUCAGAGA

TRPM8 SEQ ID NO 11 UAUUCCGUUCGGUCAUCUAdTdT SEQ ID NO 31 UAUUCCGUUCGGUCAUCUA

Sequence TRPM8b SEQ ID NO 12 UAGAUGACCGAACGGAAUAdTdT SEQ ID NO 32 UAGAUGACCGAACGGAAUA CD36 SEQ ID NO 13 UACAGACAGUUUUGGAUCUdTdT SEQ ID NO 33 UACAGACAGUUUUGGAUCU

SEQ ID NO 14 AGAUCCAAAACUGUCUGUAdTdT SEQ IDNO 34 AGAUCCAAAACUGUCUGUA integπnB3 SEQIDNO 15 GGAGAAUCUGCUGAAGGAUdTdT SEQ IDNO 35 GGAGAAUCUGCUGAAGGAU

SEQ ID NO 16 AUCCUUCAGCAGAUUCUCCdTdT SEQ ID NO 36 AUCCUUCAGCAGAUUCUCC

TRPV2 SEQ ID NO 17 UAAGAGUCAACCUCAACUAdTdT SEQ ID NO 37 UAAGAGUCAACCUCAACUA

SEQ ID NO 18 UAGUUGAGGUUGACUCUUAdTdT SEQ IDNO 38 UAGUUGAGGUUGACUCUUA

TRPV6 SEQIDNO 19 GGAAGACAGGCAAGAUCUCdTdT SEQ IDNO 39 GGAAGACAGGCAAGAUCUC

SEQ IDNO 20 GAGAUCUUGCCUGUCUUCCdTdT SEQ IDNO 40 GAGAUCUUGCCUGUCUUCC

TABLE 1

The oligonucleotide sequences numbered from 1 to 20 comprise at the 3' end of each of the two said oligonucleotide sequences two deoxythymidines extending beyond the hybrid The oligonucleotide sequences numbered from 21 to 40 do not comprise any nucleotide extending beyond the hybrid; the first oligonucleotide sequence (antisense strand) and the second oligonucleotide sequence (sense strand) have the same length, in Table 1, according to an advantageous embodiment of the invention.

The TSPl expression in humans can be inhibited using either one of the following four different double stranded oligonucleotides which target the TSPl mRNA

- the double-stranded oligonucleotide consisting in SEQ ID NO: 1 and SEQ ID NO:2, which targets the portion of the TSPl mRNA denoted TSPIa in table 1

- or the double- stranded oligonucleotide consisting in SEQ ID NO: 21 and SEQ ID NO: 22, which targets the portion of the TSPl mRNA denoted TSPIa in table 1

- or the double- stranded oligonucleotide consisting in SEQ ID NO: 3 and SEQ ID NO:

4, which targets the portion of the TSPl mRNA denoted TSPIb in table 1

- or the double- stranded oligonucleotide consisting in SEQ ID NO: 23 and SEQ ID NO: 24 which targets the portion of the TSPl mRNA denoted TSPIb in table 1.

Since the sequence denoted TSPIa in table 1 is fully conserved between several mammals, notably between human and mouse, the double-stranded oligonucleotide consisting in SEQ ID NO: 1 and SEQ ID NO: 2, or the double-stranded oligonucleotide consisting in

SEQ ID NO: 21 and SEQ ID NO: 22 can be used to inhibit TSPl expression in humans but also in other mammals. The TRPV3 expression in humans can be inhibited using either one of the following four different double stranded oligonucleotides which target the TRPV3 mRNA: -the double-stranded oligonucleotide consisting in SEQ ID NO: 5 and SEQ ID NO: 6, which targets the portion of the TRPV3 mRNA denoted TRPV3a in table 1

-or the double-stranded oligonucleotide consisting in SEQ ID NO: 25 and SEQ ID NO: 26, which targets the portion of the TRPV3 mRNA denoted TRPV3a in table 1

-or the double-stranded oligonucleotide consisting in SEQ ID NO: 7 and SEQ ID NO:

8, which targets the portion of the TRPV3 mRNA denoted TRPV3b in table 1

-or the double-stranded oligonucleotide consisting in SEQ ID NO: 27 and SEQ ID NO: 28 which targets the portion of the TRPV3 mRNA denoted TRPV3b in table 1

Since the sequence denoted TRPV3b in table 1 is fully conserved between several mammals, notably between human and mouse, the double-stranded oligonucleotide consisting in SEQ ID NO: 7 and SEQ ID NO: 8, or the double-stranded oligonucleotide consisting in

SEQ ID NO: 27 and SEQ ID NO: 28 can be used to inhibit TRPV3 expression in humans but also in other mammals. A double-stranded oligonucleotide which does not recognize any mammalian mRNA known to this day is used as a control in some in vitro and in vivo experiments. This double- stranded oligonucleotide consists in the following couple of sequences:

Strand 1 : 5'-GAUAGCAAUGACGAAUGCGUAdTdT-S'

Strand 2: 5'-UACGCAUUCGUCAUUGCUAUCdTdT-S'

It is to be noted that other controls can be substituted, and in vivo injection of the vehicle (PBS) is also used as a control.

In an advantageous embodiment, the double-stranded oligonucleotide is chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 13 and SEQ ID NO: 14) ; (SEQ ID NO: 15 and SEQ ID NO: 16); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 33 and SEQ ID NO: 34); (SEQ ID NO: 35 and SEQ ID NO: 36); (SEQ ID NO: 37 and SEQ ID NO: 38).

In a more advantageous embodiment, the double-stranded oligonucleotide is chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 37 and SEQ ID NO: 38).

To develop an oligonucleotide as a therapeutic drug inhibiting a given mRNA in humans, it is particularly advantageous that the double stranded oligonucleotide targets a sequence fully conserved between humans and mouse. This property allows of evaluation of the oligonucleotide efficiency and toxicity in preclinical models before its administration to humans. In a particular advantageous embodiment, the double-stranded oligonucleotide is chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2); (SEQ

ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 27 and SEQ ID NO: 28).

In another embodiment, the invention relates to the use of a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined,

- at least an anti-angiogenic agent,

for the manufacture of a combination product for a simultaneous, separate, or spread over time use for the prevention or the treatment of primary tumors or invasive or metastatic tumors.

- at least a double-stranded oligonucleotide above-defined,

- at least an anti-tumoral agent,

- at least a double-stranded oligonucleotide above-defined,

- at least an anti-angiogenic agent,

- at least an anti-tumoral agent,

In an advantageous embodiment, the drug is combined with an anti-tumoral therapy, such as radiotherapy or chemotherapy. In an advantageous embodiment, the anti-angiogenic agent is chosen from the group comprising Cilengitide, Vandetanib, Lenalidomide, Thalidomide, Arsenic Trioxide, Bevacizumab, anti- VEGFR- 1 , anti-VEGFR-2, anti-PDGFR, anti-FMS-FLT-3, anti-TKl.

In an advantageous embodiment, the anti-tumoral agent is chosen from the group comprising alkylating agents, such as Bendamustine, Temozolomide, Mechlorethamine, Cyclophosphamide, Carmustine, Cisplatine, Busulfan, Thiotepa, or Decarbazine, antimetabolite agents, such as Pentostatine, Methotrexate, Pemetrexed, Floxuridine, Fluorouracil, Cytaraine, Mercaptopurine or Thiguanine, cytotoxic antibiotics such as Rubitecan, Mitomycine C, Daunorubicin, Doxorubicine, Bleomycin, Plicamycin, Mitoxantrone HCl, or Oxaliplatine, plant derivatives, such as Vinorelbine, BMS 184476, Vincristine sulfate, Vinblastine, Docetaxel taxol.

In another advantageous embodiment, the primary tumor or invasive of metastatic tumor is a solid tumor or a lymphoproliferative tumor.

In a more advantageous embodiment, the solid tumor is a prostate tumor, a liver tumor, hepatic adenomas, focal nodular hyperplasia, a brain tumor such as glioma, a breast tumor, a kidney tumor, a lung tumor such as non-small cell lung carcinoma, small cell lung carcinoma, pleuropulmonary blastoma and carcinoid tumor, a bone tumor such as osteoma, osteochondroma, aneurysmal bone cyst, and fibrous dysplasia, osteosarcoma, chondrosarcoma, Ewing's sarcoma, malignant fibrous histiocytoma, fibrosarcoma, a stomach cancer, a colon tumor, a small bowel tumor, a esophageal tumor, a pancreatic tumor, a sarcoma, a cervical tumor, a gall blader tumor, a melanoma.

In another more advantageous embodiment, lymphoproliferative tumor is Leukemia, Lymphoma, or a Multiple myeloma.

In conformity with the present invention, the inhibitor of protein activity can be an antibody against TSPl protein or one protein controlling the expression or mediating the activity of TSPl, such as TRPV2, TRPV3, TRPV6, TRPM8, CD36, B3 integral, in instance.

In another aspect, the invention relates to a pharmaceutical composition.

In a general embodiment, the pharmaceutical composition comprises as active substance, at least

- one inhibitor of protein expression, which inhibits the expression of:

- TSPl protein, or

- a protein, which controls the expression of TSPl or mediates the activity of TSPl or,

- one inhibitor of protein activity, said inhibitor inhibiting the activity of: - the TSPl protein, in particular the activity responsible for the stimulation of cell migration, or

- one protein which controls the expression or mediates the activity of TSPl, in association with a pharmaceutically acceptable vehicle.

In an advantageous embodiment, the inhibitor of protein expression inhibits the expression of proteins chosen from the group comprising TSPl, TRPV2, TRPV3, TRPV6, TRPM8, CD36, or B3 integrin.

In a more advantageous embodiment, the inhibitor of protein expression inhibits the expression of proteins chosen from the group comprising TSPl, TRPV2, TRPV3, CD36, or B3 integrin.

In a particular advantageous embodiment, inhibitor of protein expression inhibits the expression of proteins chosen from the group comprising TSPl, TRPV2, TRPV3.

In another advantageous embodiment, inhibitor of the protein expression is a double- stranded oligonucleotide or a single-stranded oligonucleotide.

In another embodiment, the inhibitor of the protein expression in the pharmaceutical composition of the present invention is:

wherein the oligonucleotide sequence (a)

- is either complementary to the oligonucleotide sequence (b),

wherein the oligonucleotide sequence (a)

- is either complementary to a target sequence belonging to the RNA or DNA molecule coding one of the proteins above-defined, the expression of which is to be inhibited,

- or a fragment of the above-defined double-stranded oligonucleotides (a) and (b), comprising two complementary fragments of the respective above-defined oligonucleotide sequences (a) and (b), provided that said fragment conserves the property of inhibiting the expression of one of the proteins above-defined. The oligonucleotide sequence (a) is preferably complementary to the oligonucleotide sequence (b), but can comprise 1-8 mismatches, particularly 5, more particularly 3 mismatches, still more particularly 1 mismatch with the oligonucleotide sequence (b).

The oligonucleotide sequence (a) is preferably complementary to the target sequence, but can comprise 1-8 mismatches, particularly 5, more particularly 3 mismatches, still more particularly 1 mismatch with the target sequence; this application is particular when the length of the target sequence is 21 nucleotides.

In another embodiment, inhibitor of the protein expression in the pharmaceutical composition of the present invention is:

- a double-stranded oligonucleotide comprising two oligonucleotide sequences,

(a) and (b), forming a hybrid, wherein each oligonucleotide sequence comprises at one of its 3' or 5' ends, one to five unpaired nucleotides forming single- stranded ends extending beyond the hybrid, wherein the part inside the hybrid of the oligonucleotide sequence (a)

- is either complementary to the oligonucleotide sequence (b),

wherein the oligonucleotide sequence (a)

- or a fragment of the above-defined double-stranded oligonucleotides (a) and

(b), comprising two complementary fragments of the respective above-defined oligonucleotide sequences (a) and (b), provided that said fragment conserves the property of inhibiting the expression of one of the proteins above-defined.

In the pharmaceutical compositions of the present invention, oligonucleotide sequence complementary to the target sequence comprises from 15 to 25 nucleotides.

In an advantageous embodiment, the double-stranded oligonucleotide comprises, at the 3' end of each of the two said oligonucleotide sequences 1 to 5 nucleotides, preferably 2 to 3 nucleotides, extending beyond the hybrid.

The nucleotides extending beyond the hybrid can be complementary or not to the target sequence.

The nucleotides extending beyond the hybrid can be any natural nucleotide.

In a more advantageous embodiment, the nucleotides extending beyond the hybrid are deoxythymidines.

In a particular embodiment, the expression of the target sequence represented by SEQ ID NO: 41 (TSPl) is inhibited by the double-stranded oligonucleotide described in the present invention.

In another particular embodiment, the expression of the target sequence represented by SEQ ID NO: 43 (TRPV3) is inhibited by the double-stranded oligonucleotide described in the present invention.

In another particular embodiment, the pharmaceutical composition of the present invention comprises as active substance, the double-stranded oligonucleotide chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 9 and SEQ ID NO: 10) ; (SEQ ID NO: 11 and SEQ ID NO: 12); (SEQ ID NO: 13 and SEQ ID NO: 14); (SEQ ID NO: 15 and SEQ ID NO: 16); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 19 and SEQ ID NO: 20); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 29 and SEQ ID NO: 30); (SEQ ID NO: 31 and SEQ ID NO: 32); (SEQ ID NO: 33 and SEQ ID NO: 34); (SEQ ID NO: 35 and SEQ ID NO: 36); (SEQ ID NO: 37 and SEQ ID NO: 38); (SEQ ID NO: 39 and SEQ ID NO: 40).

In an advantageous embodiment, the pharmaceutical composition of the present invention comprises as active substance, the double-stranded oligonucleotide chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 13 and SEQ ID NO: 14) ; (SEQ ID NO: 15 and SEQ ID NO: 16); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 33 and SEQ ID NO: 34); (SEQ ID NO: 35 and SEQ ID NO: 36); (SEQ ID NO: 37 and SEQ ID NO: 38).

In a more advantageous embodiment, the pharmaceutical composition of the present invention comprises as active substance, the double-stranded oligonucleotide chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 37 and SEQ ID NO: 38).

In a particular advantageous embodiment, the pharmaceutical composition of the present invention comprises as active substance, the double-stranded oligonucleotide chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2); (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 27 and SEQ ID NO: 28).

In an advantageous embodiment, the pharmaceutically acceptable vehicle is a saline solution.

In another advantageous embodiment, the double-stranded oligonucleotide used in the pharmaceutical composition of the present invention is coupled with cholesterol or substances enabling penetration of said double-stranded oligonucleotide into the cells.

In another more advantageous embodiment, the substances enabling penetration of said double-stranded oligonucleotide into the cells are for instance liposomes, lipid-based agents, nanoparticles, magnetic spheres, polyethyleneimine derivatives.

In an advantageous embodiment, the active substance is formulated for the administration at a dose in the range of 0, 05 to 50 mg/kg, in particular 0, 1 to 20 mg/kg.

The active substance comprising the double-stranded oligonucleotide can be administrated at a modified but sufficient dose according to the application way or the form of the active substance.

In an advantageous embodiment, the active substance is formulated for one of the following administrations: intravenous, intraperitoneal, subcutaneous, intramuscular, nasal instillation, sublingual, intra rectal, direct injection in the tumor, topical or oral. The oligonucleotides of the present invention can be either transfected in cells which are then injected in the tissues, or be directly injected in the tissues by, for example local , systemic, aerosol route.

In an advantageous embodiment, the pharmaceutical composition comprises, as active substance, a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined

- at least an anti-angiogenic agent,

as combination product for a simultaneous, separate, or spread over time use. In a preferred embodiment, the pharmaceutical composition comprises, as active substance, a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined,

- at least an anti-tumoral agent,

as combination product for a simultaneous, separate, or spread over time use.

In a preferred embodiment, the pharmaceutical composition comprises, as active substance, a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined,

- at least an anti-angiogenic agent,

- at least an anti-tumoral agent,

as combination product for a simultaneous, separate, or spread over time use.

The anti-angiogenic agent of the pharmaceutical composition of the invention can be chosen from the group comprising Cilengitide, Vandetanib, Lenalidomide, Thalidomide, Arsenic Trioxide, Bevacizumab, anti-VEGFR-1, anti-VEGFR-2, anti-PDGFR, anti-FMS- FLT-3, anti-TKl .

The anti-tumoral agent of the pharmaceutical composition of the invention can be chosen from the group comprising alkylating agents, such as Bendamustine, Temozolomide, Mechlorethamine, Cyclophosphamide, Carmustine, Cisplatine, Busulfan, Thiotepa, or Decarbazine, anti-metabolite agents, such as Pentostatine, Methotrexate, Pemetrexed, Floxuridine, Fluorouracil, Cytaraine, Mercaptopurine or Thiguanine, cytotoxic antibiotics such as Rubitecan, Mitomycine C, Daunorubicin, Doxorubicine, Bleomycin, Plicamycin, Mitoxantrone HCl, or Oxaliplatine, plant derivatives, such as Vinorelbine, BMS 184476, Vincristine sulfate, Vinblastine, Docetaxel taxol.

In another embodiment, the invention relates to the oligonucleotide sequence chosen from the following sequences: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40

All these sequences are now per se.

In another embodiment, the invention relates to the double-stranded oligonucleotides chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 9 and SEQ ID NO: 10) ; (SEQ ID NO: 11 and SEQ ID NO: 12); (SEQ ID NO: 13 and SEQ ID NO: 14); (SEQ ID NO: 15 and SEQ ID NO: 16); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 19 and SEQ ID NO: 20); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 29 and SEQ ID NO: 30); (SEQ ID NO: 31 and SEQ ID NO: 32); (SEQ ID NO: 33 and SEQ ID NO: 34); (SEQ ID NO: 35 and SEQ ID NO: 36); (SEQ ID NO: 37 and SEQ ID NO: 38); (SEQ ID NO: 39 and SEQ ID NO: 40).

In an advantageous embodiment, the invention relates to the double-stranded oligonucleotides chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 13 and SEQ ID NO: 14) ; (SEQ ID NO: 15 and SEQ ID NO: 16); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 33 and SEQ ID NO: 34); (SEQ ID NO: 35 and SEQ ID NO: 36); (SEQ ID NO: 37 and SEQ ID NO: 38).

In an advantageous embodiment, the invention relates to the double-stranded oligonucleotides chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 37 and SEQ ID NO: 38).

In a particular advantageous embodiment, the invention relates to the double-stranded oligonucleotide chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2); (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 27 and SEQ ID NO: 28).

All these double-stranded oligonucleotides are now per se.

The present invention is illustrated by the following Figures and examples, which are in no way the limitation of the scope of the invention.

DESCRIPTION OF THE FIGURES

Figure IA represents TSPl mRNA level in androgen-dependent (LNCaP), castration- resistant (C4-2 and 22RV1), or androgen-independent (PC3) prostate tumor cells. Results (mean ± SEM, n>3) are normalized to the cyclophilin A mRNA level and expressed in arbitrary units (LNCaP set to 1).

Figure IB represents corresponding TSPl protein level in androgen-dependent (LNCaP), castration-resistant (C4-2 and 22RV 1), or androgen-independent (PC3) prostate tumor cells. Tubulin was used as a loading control.

Figure 1C represents TSPl mRNA level in C4-2 cells 48h after transfection with two different siRNAs targeting the TSP 1 mRNA. Results, normalized to the cyclophilin A mRNA level in the same cells, are expressed as arbitrary unit, mean ± SEM of 3 independent experiments.

Figure ID represents the TSPl expression, visualized by indirect immunofluorescence in C4-2 cells 48h after transfection with TSP 1 a- or TSP lb-siRNA.

Figure IE represents the metabolic activity of C4-2 cells after transfection of TSPIa- or TSPlb-siRNA.

Figure IF represents the enzymatic activity of caspases 3 and 7 measured 48h after transfection of C4-2 cells with the TSPIa- or TSPlb-siRNA. Results are not statistically different between control or TSPl-siRNA transfected cells.

Figure IG represents the migration capacity towards fresh culture medium of C4-2 (black bars) or PC3 cells (grey bars) transfected with the indicated siRNAs. Two days after transfection, cells were seeded in the upper part of the Boyden chamber and the number of cells that had migrated towards fresh medium was counted 18h later. Result are expressed as the percentage of migrating cells as compared to cells migrating in the control condition (mean ± SEM, «=3). The experiment was repeated 3 times with comparable results.

Figure IH represents the migration capacity of LNCaP towards conditioned medium from LNCaP or C4-2 cells. LNCaP (black bars) or C4-2 cells (grey bars) were plated in the lower part of Boyden chambers. 2 days later, LNCaP cells were seeded in the upper chamber and the number of migrating cells counted 18h later. Results are expressed as in G.

Figure II represents TSPl expression, measured by western blotting, in cellular homogenates and in the conditioned medium of JT8 cells cultured for 2 days in the presence (dox+) or absence (dox-) of doxycycline. JT8 cells are fibrosarcoma cells stably transfected with a tet-repressible plasmid coding for TSPl .

Figure IJ represents the migration of C4-2 cells towards the medium conditioned by TSPl -inducible JT8 cells grown in the presence of doxycycline to repress TSPl expression or in absence of doxycycline to induce TSPl. Results are expressed as in G.

Figure IK represents the migration capacity of C4-2 cells transfected with the indicated siRNAs. Two days after transfection, cells were plated in the upper part of the Boyden chamber and the number of cells that had migrated towards fresh medium was counted 2 days later. Results are expressed as in Figure 1C.

Figure IL represents the migration capacity of C4-2 towards C4-2-conditionned medium in the absence or presence of an antibody which inhibits the binding of TSPl to CD36. C4-2 cells were plated in the lower part of Boyden chambers. 2 days later, C4-2 cells were plated in the upper chamber in control medium or in medium containing lμg/ml of, TSPl -antibody AbI (Clone A4.1 from neomarkers, Thermo scientific, Fremont, Ca, USA). When the TSPl-AbI antibody was added in the upper chamber, it was also added at the same concentration in the lower chamber. After a 18h incubation, migrating cells were counted.

Figure 2A represents TSP 1 expression in C4-2 cells treated for 2 h by vehicle (left) or thapsigargin (lμM) (right). TSPl expression is visualized by indirect immunofluorescence.

Figure 2B represents TSPl secretion in the cell culture medium by C4-2 cells treated for 2 h by vehicle (left) or thapsigargin (lμM) (right). The secreted TSPl is measured by western blotting in the cell culture medium.

Figure 2C represents TSPl expression in C4-2 cells treated for 2h with camphor at the indicated doses. TSPl expression is visualized by indirect immunofluorescence.

Figure 2D represents TSPl secretion in the cell culture medium by C4-2 cells treated for 2 h by vehicle or camphor at the indicated doses. The secreted TSPl is measured by western blotting in the cell culture medium.

Figure 2E represents TSPl mRNA level in C4-2 cells after 6h of treatment by camphor at the indicated doses (mean ± SEM, «=3). mRNA level is normalized to cyclophilin A. Figure 2F shows TRPV3 (black bars) and TRPM8 (grey bars) mRNA levels measured in the indicated prostate tumor cell lines. Results (mean ± SEM, «>3) are normalized to cyclophilin A mRNA level and expressed in arbitrary units.

Figure 2G represents the detection by western-blot of TRPV3 protein in indicated prostate cell lines. Tubulin was used as a loading control.

Figure 2H represents TSPl and TRPV3 expression in C4-2 cells 48h after transfection of TRPV3a siRNA.

Figure 21 represents TSPl mRNA level of C4-2 cells transfected by the indicated siRNAs. Results, normalized to cyclophilin A mRNA level are measured 2 day after transfection (mean ± SEM, n>3).

Figure 2 J represents the migration capacity towards fresh culture medium of C4-2 cells transfected with the indicated siRNAs. Cells were seeded in the upper chamber 2 days after transfection and the number of cells that had migrated was counted 18h later. Result are expressed as the percentage of migrating cells as compared to cells migrating in the control condition (mean ± SEM, «=3). The experiment was repeated 3 times with comparable results.

Figure 3A represents the effects of TRPM8 and TRPV3 silencing on C4-2 cell proliferation. C4-2 cells were transfected with the indicated siRNAs. Their proliferation is measured by a metabolic assay (mean ± SEM, o=3, representative of 3 separate experiments).

Figure 3B represents the effects of TRPV3 silencing on the migration of C4-2 (black bars) or PC3 cells (grey bars). Cells were transfected by control, or 2 different TRPV3 siRNA as indicated, and a migration assay performed as in Fig IG.

Figure 3C represents the migration capacity of C4-2 cells transfected 2 days before with a cont- or TRPC4- or TRPC6-siRNA.

Figure 3D represents Western blot detection of TSPl in the culture medium of C4-2 cells transfected with cont- or TSPl -siRNA 48h after transfection..

Figure 3E represents the effects of TSPl on migration capacity of C4-2 cells. C4-2 cells plated in the lower part of a Boyden chamber were transfected by either control- (noted C), or TSPl-siRNA (TSP). On the same day, a separate batch of cells was transfected by either control-, TSPl-, or TRPV3-siRNA (TRP). Three days later, these latter cells were trypsinized and seeded into the upper part of the Boyden chamber as indicated. Migration was quantified 18h later and results expressed as a percentage of cells migrating in the control condition (cells transfected with a control-siRNA in the upper and lower part) (mean ± SEM, /i=3). Figure 4 A represents TRPV3 (light grey bars) and TSPl mRNA levels (dark grey bars) measured in C4-2 cells incubated in normoxia or in the presence of cobalt chloride

300μM to mimic hypoxia. mRNA levels are quantified and normalized to cyclophilin A. Results are expressed as the ratio of mRNA levels in hypoxia to that in normoxia set to 1. The results from 2 independent experiments are shown.

Figure 4B represents intracellular calcium concentration in C4-2 cells grown in control conditions (cont) or in the presence of 300μM C0CI₂ for 30 minutes or 48 hours (mean ± SEM, n>120 cells per condition).

Figure 4C represents VEGF and TSPl mRNA levels in C4-2 cells incubated in 20%

(normoxia) or 1% oxygen (hypoxia) for up to 72h. Light grey bars represent VEGF in normoxia; dark grey bars represent VEGF in hypoxia; white bars represent TSPl in normoxia; black bars represent TSPl in hypoxia. Results are normalized to the cyclophilin A mRNA level and expressed in arbitrary units, TO set to 1.

Figure 4D represents VEGF and TSPl protein content in C4-2 cell homogenates measured by ELISA and normalized to total protein content. Light grey bars represent VEGF in normoxia; dark grey bars represent Color code as in C.

Figure 4E represents VEGF and TSPl protein content in C4-2 cell culture medium, measured by ELISA and normalized to total protein content in cell homogenates. Color code as in C.

Fig 4F represents TSPl, Hifl alpha and TRPV3 protein content in C4-2 cell homogenates in cells grown for the indicated periods in normoxia (N) or hypoxia (H) Tubulin was used as a loading control.

Fig 4G represents the induction of VEGF (grey) and TSPl (black) mRNA levels normalized to cyclophilin A levels in PC3 cells grown for the indicated periods in cobalt chloride as compared to the respective mRNA levels in control medium at the same time point.

Figure 4H represents TSPl immunodetection in PC3 cells grown for 48h in control conditions (N) or in the presence of 300 μM CoC12 to mimic hypoxia (H).

Figure 5A represents tumor volume of mice bearing exponentially growing C4-2 tumors. Mice were treated daily with i.p. injections of PBS (triangles), Control- (black squares) TRPV3- (light grey diamonds) or TSPl-siRNA (dark grey diamonds). All siRNAs were diluted (120μg/kg) in PBS. Tumor volume is expressed in cm³ (mean ± SEM, 6 mice per group).

Figure 5B represents tumor volume of mice bearing exponentially growing PC3 tumors. Mice were treated daily with i.p. injections of Control- (black squares) TRPV3- (light grey diamonds) or TSPl-siRNA (dark grey diamonds). All siRNAs were diluted (120μg/kg) in PBS. Tumor volume is expressed in cm³ (mean ± SEM, 6 mice per group).

Figure 5C represents TRPV3 and TSPl mRNA levels, normalized to cyclophilin A and expressed in arbitrary units, in PC3 tumors collected at the end of the experiment depicted in Figure 5B. The TSPl mRNA level was plotted against the TRPV3 mRNA level in the same tumor from mice treated with control-siRNA (black squares), TRPV3-siRNA (grey diamonds) or TSPl-siRNA (white circles).

Figure 5D represents the TSPl mRNA level, normalized to cyclophilin A mRNA level, in tumors collected at the end of experiments shown in Fig 5A (C4-2, diamonds) and 5B (PC3, triangles). Tumors were treated by control- siRNA (black symbols), or TRPV3- siRNA (light grey symbols) or TSPl-siRNA (dark grey symbols).

Figure 5E represents the TRPV3 mRNA level, normalized to cyclophilin A mRNA level, in tumors collected at the end of experiments shown in Fig 4A (C4-2, diamonds) and 4B (PC3, triangles). Tumors were treated by control- siRNA (black symbols), or TRPV3- siRNA (light grey symbols) or TSPl-siRNA (dark grey symbols).

Figure 5F represents the quantification of microvessel density (MVD) in hot spots of vascularization from C4-2 (grey bars) or PC3 (black bars) tumors collected at the end of the experiment described in Figure 5A and Figure 5B.

Figure 5 G represents the VEGF mRNA level, normalized to cyclophilin A mRNA level, in tumors collected at the end of experiments shown in Fig 4A (C4-2, diamonds) and 4B (PC3, triangles). Tumors were treated by control- siRNA (black symbols), or TRPV3- siRNA (light grey symbols) or TSPl-siRNA (dark grey symbols).

Figures 5H represents mRNA level of the indicated genes in sacrificed nude mice 5 hours after an i.p. injection of PBS (black bars), or 4000 μg/kg of either po Iy(LC), a known ligand of TLR3 (dark grey bars), or TRPV3b-siRNA (light grey bars) or TSPla-siRNA (white bars) all diluted in PBS. The mRNA level of the indicated genes, normalized to cyclophilin A, was quantified by real time RT-PCR in the spleen. Results (mean ± SEM, «=4) are expressed in arbitrary units, normalized to the value in PBS controls set to 1. Figure 6A represents TSPl mRNA level, normalized to actin mRNA, in peritumoral

(N) and tumoral (T) tissues from frozen radical prostatectomy samples of different clinical stages (pT2, pT3a, pT3b/pT4) taken before any other treatment. Each box plot is composed of three horizontal lines that display the 25th, 50th (median), and 75th percentiles. The highest and lowest values are shown using error bars. P value is shown when significant.

Figure 6B represents the comparison of TSPl mRNA level, normalized to actin mRNA, in samples from patients who did not show evidence of tumor recurrence during at least 30 months following surgery (n=8) or who experienced PSA relapse (n=l l) after surgery.

EXAMPLES

The following examples have been carried out according to the experimental procedures hereafter described.

Reagents and siRNAs

Camphor and thapsigargin were purchased from Sigma-Aldrich (Saint-Quentin

Fallavier, France). TSPl antibodies (AbI, Ab-4 and Ab-I l) were from Neomarkers (Thermo scientific, Fremont, Ca, USA ), TRPV3 antibodies from TEBU (Le Perray en Yvelines, France) and Tubulin from Sigma-Aldrich (Saint-Quentin Fallavier, France). Alexa-Fluor goat anti-rabbit 488 and Alexa-Fluor goat anti-mouse 568 were purchased from Molecular probes. SiRNAs were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). The sequences used are indicated in the supplementary table 1. Cell lines

Cell line LNCaP is a human androgen-dependent prostate cell line. It expresses the androgen receptor and depends of androgen for its growth.

Cell line C4-2 and 22RV1 are human castration-resistant prostate tumor cell lines. They express the androgen receptor but no more depend on androgens for their growth.

Cell line PC3 is a human androgen- independent prostate cell line. PC3 cells no longer express the androgen receptor.

Cell culture and transfection

LNCaP and C4-2 cells were grown in RMPI containing 10% fetal calf serum, PC-3 cells in DMEM containing 10% fetal calf serum. The Hiperfect reagent (Qiagen, Courtaboeuf, France) was used to trans feet cells in 24 wells plates with the indicated siRNAs (1OnM) as recommended by the manufacturer. A metabolic activity assay (WSTl, Roche Diagnostics, Meylan France) was used to measure cell proliferation. To mimic hypoxia, cells were grown in the presence of 300 μM cobalt chloride for 48h. For hypoxic conditions, cells were cultured at 37°C with 5% CO2, 94% N2 and 1% 02 in a hypoxic incubator (Binder GmbH, Tuttligen, France).

Migration assay

The migration capacity was measured using a modified Boyden Chamber. Cells (40,000) were seeded in RPMI 1% FBS in the upper part of a cell culture-chamber- insert system separated from the lower chamber by a 8 μm PET membrane (BD Biosciences, Le Pont de Claix, France). RPMI 10% FBS, or conditioned medium was added in the lower compartment. Eighteen hours later, non migrating cells in the upper compartment were scrapped off using a cotton swab. Cells on the lower side of the membrane were fixed with methanol at -20⁰C and stained with Hoechst 33258 (Sigma-Aldrich, Saint-Quentin Fallavier, France). Membranes were then excised, mounted on a glass side with Glycergel (DAKO) and cells counted.

Real-time RT-PCR siRNA and mRNA analysis

Total RNA was isolated using TRIzol reagent (Invitrogen, Cergy Pontoise, France).

RNA were retrotranscribed using a High capacity cDNA Reverse Transcription Kit (Applied Biosystems, Courtaboeuf, France). cDNA was quantified by real time PCR using the Power SYBR® Green PCR Master Mix (Applied Biosystems, Courtaboeuf, France). Human Cyclophilin A was used as an internal control. The sequences of the PCR primers are indicated in the supplementary table 2.

ELISA

TSPl and VEGF protein contents in cell homogenates and supernatant were measured by ELISA (quantikine, R&D, Lille, France).

Calcium Imaging

Fluorescence imaging was carried out in Hank's balanced salt solution (HBSS) containing 142mM NaCl, 5.6 mM KCl, 1 mM MgCl₂, 2 mM CaCl₂, 0.34 mM Na₂HPO₄, 0.44 mM KH2PO4, 10 mM HEPES, and 5.6 mM glucose. Cytosolic calcium concentration was measured using Fura-2 loaded cells (2 μM) as previously described (Mariot et al, 2002). The intracellular calcium concentration was derived from the ratio of the fluorescence intensities for each of the excitation wavelengths (F340/F380) and from the Grynkiewicz equation (Grynkiewicz et al., 1985).

Animals, siRNA injection and tumorigenicity assays

Studies involving animals, including housing and care, method of euthanasia and experimental protocols were conducted in accordance with the local animal ethical committee in the Institut Andre Lwoff in Villejuif, France. Tumor cells (2><10⁶ cells/mouse) were injected subcutaneously in 50% (v:v) matrigel (BD biosciences, Le Pont de Claix, France) to 6-8 weeks old male nude mice and measured every day. When tumors grew exponentially, siRNA diluted in PBS were injected i.p. on a daily basis (120μg/kg). Tumor volume was estimated using the formula: length x width² x 0.5. Subjects

Prostate tissue samples were obtained from 14 patients who underwent radical prostatectomy at the Centre Hospitalier Lyon Sud (Lyon, France) and 12 from the Cochin Hospital (Paris, France). Written consent was obtained from each patient. Immediately after prostate removal small pieces of tissues were gross dissected by the pathologist, snap-frozen and stored in liquid nitrogen until analysis in tumor banks of the Centre hospitalier Lyon Sud and of the groupe hospitalier Cochin-Saint Vincent de Paul. Histological analysis of a frozen section was performed for each sample by the same pathologist before RNA extraction. The fragments fully constituted of cancerous glands were selected and named "tumor" samples, whereas those that did not contain cancerous tissue were selected and named "peritumoral tissue".

Example 1: TSPl expression is increased in hormone-refractory cancer prostate cells

The TSPl promoter is methylated in the androgen-dependent prostate cell line LNCaP (Li et al., 1999) and accordingly, TSPl mRNA and protein levels are very low in these cells (Fig IA and Fig IB). Interestingly, a sustained TSPl expression was found in C4-2 cells, which were established from LNCaP tumors recurring in mice after castration (Thalmann et al., 1994). TSPl was also found expressed, at the mRNA (Fig IA) and protein (Fig IB) levels, in the castration-resistant prostate tumor cell line R22RV1 (Sramkoski et al., 1999), and in the androgen- independent cell line PC3. To study the function of TSPl in prostate carcinoma cells, a first siRNA was designed to target a sequence fully conserved between the mouse and the human sequences (TSPla-siRNA) and a second one was designed to target specifically the human mRNA (TSPlb-siRNA). In C4-2 cells, the two siRNAs silenced TSPl expression over 70% at the mRNA (Fig 1C) and protein level (Fig ID), with no effect on cell proliferation (Fig IE) or apoptosis (Fig IF).

Example 2: TSPl stimulates the migration of prostate tumor cells.

Boyden chambers were used to study a potential role of TSPl on the migration of prostate tumor cells. TSPl silencing strongly inhibited the migration of C4-2 cells (Fig IG). This effect was not dependent upon the expression of the androgen receptor because the migration of PC3 cells was also strongly affected by TSPl silencing (Fig IG). Conversely, the migration of LNCaP cells, which do not express TSPl, was stimulated when cells migrated towards C4-2 cells-conditioned medium (Fig IH). To further establish the role of TSPl on the migration of prostate tumor cells, a cell line, JT8, where the production of TSPl is under the control of a tetracycline-repressible promoter was used (Filleur et al., 2001). Conditioned medium of JT8 cells was prepared in the presence of doxycycline to repress TSPl expression, or in its absence to induce TSPl expression (Fig II). The migration of C4-2 cells towards these two media was then measured. The presence of TSPl strongly increased the capacity of cells to migrate (Fig IJ).

The activity of TSPl is mediated by several kinds of receptors, in particular integrins comprising the subunits βl or β3, and the CD36 receptor. Binding of TSPl to CD36 has been shown to mediate the antiangiogenic effects of TSPl. In the present invention, specific siRNAs were designed to target respectively CD36, βl and β3 integrins. Silencing βl had no effect on the migration properties of C4-2 cells (Fig IK). In contrast, silencing CD36 or β3 strongly reduced the C4-2 migration in the Boyden assay (Fig IK),. In addition, TSPl antibodies that inhibit its binding to the CD36 receptor (TSPl AbI, clone A4.1) impaired the migration of C4-2 cells (Fig IL), demonstrating that the binding of TSPl to CD36 mediates the antiangiogenic effects of TSPl and its capacity to induce migration.

The above mentioned results show that TSPl is expressed at much higher levels

(mRNA and protein) in C4-2, 22RV 1 and PC3 cells than in LNCaP cells. Transfection of C4- 2 cells by 10 nM of TSPla-siRNA or TSPlb-siRNA significantly reduces the TSPl mRNA level (Fig 1C) and protein level (Fig ID), without affecting cell proliferation (Figure IE) . Example 3: TSPl expression and secretion are regulated by calcium in prostate tumor cells.

TSPl contains a calcium-binding domain and calcium affects TSPl folding (Adams, 2004). The question is whether an increase in the cytosolic calcium concentration in prostate tumor cells could regulate TSPl expression and/or secretion. For this purpose, C4-2 cells were treated with thapsigargin, an inhibitor of SERCA pump, which increases the intracellular calcium concentration (Ca⁺^). A 2h treatment with thapsigargin resulted into a rapid depletion of TSPl from the cytosol (Fig 2A) and secretion into the culture medium (Fig 2B). The calcium channels TRPV2 (Monet et al., 2010), TRPV6 (Fixemer et al, 2003), TRPM8 (Fig. 2J), and TRPV3 (Fig. 2F and 2G) are expressed in prostate tumor cells. Camphor is a well- established agonist of TRPV3 channel (Moqrich et al., 2005; Vogt-Eisele et al., 2007) similarly stimulated TSPl secretion (Fig 2C and 2D). In addition, a 6h treatment by camphor induced a dose dependent increase in TSPl mRNA level (Figure 2E). These results establish that calcium regulates both TSPl mRNA level and secretion in prostate tumor cells.

Example 4: Expression of TRP channels and regulation of TSPl in prostate tumor cells.

The expression of two TRP channels, TRPM8 and TRPV3, was analysed in prostate cell lines. TRPM8 was expressed in LNCaP and C4-2 cells but undetectable in 22RV 1 and at a very low level only in PC3 cells (Fig 2F). In contrast, TRPV3 was expressed in all these 4 prostate cell lines (Fig 2F), including the androgen-independent PC3 cells. The TRPV3 mRNA was upregulated in the castration-resistant cell line C4-2 as compared to its parental androgen-dependent cell line LNCaP. However, the results of invention clearly show that, at the protein level, TRPV3 is strongly expressed in LNCaP cells (Fig 2G). Then it was analysed whether the expression of TRP channels could regulate TSPl expression. Silencing TRPV3 reduced TSPl protein expression (Fig 2H). At least 4 channels of the TRP family, TRPV2, TRPV3, TRPV6 and TRPM8, stimulate TSPl, as their silencing resulted into a reduced TSPl mRNA level (Fig 21). In contrast, TRPC4 and TRPC6 repressed TSPl, and their silencing increased its mRNA level in C4-2 cells (Fig 21, 2J). TRPCl, TRPC3 or ORAI had no significant effect on TSPl expression (Fig 21).

Example 5: TRPV3 channel is involved in the control of prostate cancer cell migration To address the possible role of TRP calcium channels on migration, focus was made on TRPV3 as this channel is well expressed in prostate carcinomas cells, whatever their dependence on androgens, and because, as opposed to TRPM8, TRPV3 silencing does not affect cell proliferation (Fig 3A), a phenotype which could bias the interpretation of migration assays. TRPV3 silencing triggered a massive inhibition of cell migration in C4-2 and PC3 cells (Fig 3B). In contrast, siRNAs targeting TRPC4 or TRPC6 stimulated C4-2 cells migration (Fig 3C). All together, these results strongly suggest that the effects of TRP channels on migration could be mediated by TSPl. Example 6: The effects of TRPV3 on cell migration are mediated by TSPl

To further study the respective roles of TSP 1 and TRPV3 in cell migration, C4-2 cells plated in the lower part of Boyden chambers were transfected by either control- or TSPl- siRNAs. Three days later, the TSPl concentration was markedly reduced in the conditioned medium of TSPl-siRNA transfected cells (Fig 3D). We then added in the upper part of the chambers C4-2 cells transfected 3 days earlier with control-, TSPl-, or TRPV3-siRNAs. As compared to control conditions (cells transfected by control siRNAs in the upper and lower chambers), silencing TSPl simultaneously in the two compartments reduced migration by 70% (Fig 3E). Interestingly, when TSPl was silenced in a single compartment, either upper or lower, the migration capacity was only partially reduced, demonstrating that the migration effect is mediated by the secreted protein (Fig 3E).

Silencing TRPV3 in the upper compartment drastically repressed the migration of C4- 2 cells migrating towards a medium depleted in TSPl. But importantly, this inhibition was completely abolished when TSPl was present in the lower part of the Boyden chambers (Fig 3E). This result demonstrates that the stimulation by TRPV3 of cell migration is mediated by the secreted TSPl.

Example 7: Hypoxia induces the expression of TRP V3 and TSPl and increases

Resistance to hypoxic conditions is a common feature of advanced tumors. The TSPl and TRPV3 mRNA levels, and the intracellular calcium concentration, were analysed in C4-2 cells under hypoxic conditions. A 48h-treatment by 300 μM cobalt chloride, which induces the stabilization of Hifla and Hif2a proteins (Yuan et al., 2003) and mimics the effects of hypoxia, strongly induced the TRPV3 and TSPl mRNA levels (Fig 4A). The intracellular calcium concentration was measured in C4-2 cells grown in control conditions or in the presence of cobalt chloride. The resting level, which was not modified after 30 min, was increased over two fold in cells incubated for 48h in the presence of cobalt chloride (Fig 4B).

TSPl is induced by hypoxia in human fibroblasts and vascular smooth muscle cells

(Distler et al., 2007; Favier et al., 2005; Mayuko Osada-Oka, 2008). To analyze whether this was also the case in prostate tumor cells, C4-2 cells were grown in 1% or 20% oxygen, and VEGF and TSPl expressions were compared at different time points up to 72h. A time- dependent increase of both VEGF and TSPl mRNA was observed in hypoxia at the mRNA (Fig. 4C) and protein level (Fig. 4D). As soon as 2h after exposure to hypoxia, the secretion of TSPl protein was induced (Fig. 4E). TRPV3 and Hiflalpha proteins were also induced by hypoxia in a time-dependent manner (Fig 4F). Similarly, exposure of PC3 cells to CoC12, which mimics the effects of hypoxia, induced TSPl expressions at the mRNA (Fig. 4G) and protein levels (Fig. 4H).

Example 8: In vivo silencing of TRPV3 or TSPl inhibits the growth of castration- resistant or androgen-independent prostate tumors.

The In vitro data of the invention establish that the migration of C4-2 or PC3 cells is strongly impaired by TRPV3 or TSPl silencing, without affecting cell proliferation or survival. In order to address the role of TSPl and TRPV3 In vivo, C4-2 cells were xenografted into nude mice. Once tumors were exponentially growing, mice were randomized for treatment and received daily either PBS i.p. injections or 120 μg/kg of either control-, or TSPl-, or TRPV3-siRNA diluted into PBS and injected i.p. The growth of tumors in mice treated with TSPl- or TRPV3- siRNA was significantly inhibited (Fig 5A). Similarly, both TRPV3- and TSPl-siRNAs inhibited the growth of xenografted PC3 tumors (Fig 5B).

In control PC3 tumors collected at the end of the experiment depicted in Fig 5B, a high degree of correlation was observed (r²=0.83) between the TRPV3 and TSPl mRNA levels (Fig 5C). As compared to controls, treatment by TSPl-siRNA significantly decreased the TSPl mRNA level in PC3 tumors (Fig 5C). Of note, silencing TRPV3 resulted into a reduction of TRPV3 mRNA level, but also into a reduction of TSPl mRNA level (Fig 5C). These data confirm that, In vivo as In vitro, TRPV3 regulates TSPl expression.

In C4-2 tumors, treatment by TRPV3- or TSPl-siRNA significantly decreased the corresponding target mRNA level as compared to controls (Fig. 5D, 5E). Example 9: TSPl still exerts antiangiogenic properties in castration-resistant tumors

Although C4-2 and PC3 tumors treated by TSPl- or TRPV3-siRNA were smaller and highly necrotic, their microvessel blood density (MVD) in non-necrotic regions was significantly higher than in controls (Fig 5F), showing that TSPl still repressed angiogenesis in CRCaP and AICaP tumors. The increased MVD paralleled a reduced VEGF expression in TSPl-siRNA treated tumors (Fig 5G), indicative of a reduced hypoxia. This result establishes that TSPl still exerts antiangiogenic properties in C4-2 and PC3 tumors. Example 10: Absence of induction of the expression of interferon or inflammatory cytokines.

In order to confirm that the antitumoral effects observed in vivo were not linked to a non-specific immune response, mice were given one injection by intraperitoneal route of TSPla-siRNA, or of TRPV3b-siRNA, or of Poly (I: C), a known ligand of TLR3, used as a positive control. All the injections were carried out with a dose of 4mg/kg siRNA diluted in PBS. Five hours after injection, mRNAs coding several genes involved in innate immune response or in inflammation were quantified by quantitative real time RT-PCT. Only the treatment by Poly (I: C) induces a significant increase of TLR3, TLR7, IL6, IL12b, IFNβ, IFNγ, and IPlO, whereas neither TSPl-siRNA nor TRPV3-siRNA at the same dosage, 33 fold higher than that used in tumor experiments, induced these TLR and cytokines (Fig 5H). These results establish that the antitumor effect observed by injections of TSPl-siRNA or TRPV3- siRNA in vivo cannot be attributed to a stimulation of innate immunity.

Example 11: TSPl expression is associated with pathological stage and cancer recurrence after radical prostatectomy.

TSPl mRNA expression was studied in 26 frozen radical prostatectomy specimens from patients with clinically localized prostate cancer who did not receive any radiotherapy and/or hormonal ablation treatment before surgery. Pairs of tumor and peritumoral tissue were analyzed in 18 specimens. The mean TSPl mRNA level was significantly higher in peritumoral tissue than in tumors (Table 1), confirming that TSPl expression is repressed in untreated androgen-dependent tumors.

There was no significant association between tumoral or peritumoral TSPl mRNA level and patients' age, Gleason score or serum PSA level before surgery (Table 1). In tumoral tissue, TSPl mRNA level was significantly lower in patients with localized disease (pT2) as compared to those with locally advanced prostate cancer (pT3) (Table 1 and Fig. 6A). In peritumoral tissue, there is a trend towards higher TSPl mRNA level in patients with higher stage disease although the difference was not statistically significant.

Of the 26 patients included in this study, 11 experienced PSA relapse, whereas 8, followed for at least 30 months after surgery, did not show evidence of tumor recurrence. TSPl mRNA level, measured in tumoral and peritumoral tissue at the time of radical prostatectomy, was significantly associated with PSA relapse (Table 1 and Fig. 6B).

Table 1

TSPl/actin mRNA level (a.u.)

Peritumoral tissue (N) Tumor (T)

Criteria No Mean [range] p No Mean [range] P p T vs N All patients 18 388 [026 - 1354] 26 125 [020-715] 0.004 age

<=60 8 494 [049 - 1354] 13 161 [020-715] 0.028 >60 IO 304 [026 -1281] 0197 13 090 [023 - 204] 0110 0.028

Gleason score

6 2 053 [026 081] 6 115 [031 253] 0191

7 13 470 [049 - 1354] 0139⁺ 15 131 [020-715] 0419⁺ 0.011 >7 3 258 [082 - 502] 015O⁺ 5 120 [023 -213] 0459* 0118

PSA before surgery (ng ^■M)

<7 6 501 [026-129] 10 164 [024 -715] 0063

7-15 6 127 [049 -217] 0082° 8 095 [020-253] 0195° 0219

>15 5 544 [082 - 1354] 0452° 7 120 [028 - 260] 0306° 0.025 unknown 1 502 1 023

stage

pT2 115 [049-151] 8 041 [020-093] 0.002 pT3a 283 [026-631] 0114* 7 140 [028 -275] 0.012* 0098 pT3b 577 [081 1354] 0085* 10 193 [033 715] 0.023* 0.039 pT4 502 1 023

Tumor relapse (follow-up in months, mean [range])

no 51 [3064] 6 134 [026 -210] 8 064 [030 - 141] 0.014 yes 36[6-8O] 7 746 [081 -1354] 0.012 10 219 [023- 715] 0.022 0.007

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Claims

1. Use of at least:

- one inhibitor of protein expression, which inhibits the expression of:

- TSPl protein, or

or,

- one inhibitor of protein activity, said inhibitor inhibiting the activity of:

- one protein which controls the expression or mediates the activity of TSPl, for the manufacture of a drug for the prevention or the treatment of primary tumors or invasive or metastatic tumors.

2. Use according to claim 1, wherein the inhibitor of protein expression inhibits the expression of proteins chosen from the group comprising TSPl, TRPV2, TRPV3, TRPV6, TRPM8, CD36, or B3 integrin.

3. Use according to claims 1 or 2, wherein the inhibitor of the protein expression is:

- a double-stranded oligonucleotide comprising two oligonucleotide sequences,

(a) and (b), forming a hybrid,

wherein the oligonucleotide sequence (a)

- is either complementary to the oligonucleotide sequence (b),

wherein the oligonucleotide sequence (a)

- or presents less than 40% mismatches with a target sequence belonging to the RNA or DNA molecule coding one of the proteins above- defined, the expression of which is to be inhibited, - or a fragment of the above-defined double-stranded oligonucleotides (a) and (b), comprising two complementary fragments of the respective above-defined oligonucleotide sequences (a) and (b), provided that said fragment conserves the property of inhibiting the expression of one of the proteins above-defined, or

- is either complementary to the oligonucleotide sequence (b),

wherein the oligonucleotide sequence (a)

- is either complementary to a target sequence belonging to the RNA, or

DNA molecule coding one of the proteins above-defined, the expression of which is to be inhibited,

4. use of a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined in claims 1 to3,

- at least an anti-angiogenic agent,

for the manufacture of a combination product for a simultaneous, separate, or spread over time use for the prevention or the treatment of primary tumors or invasive or metastasic tumors,

or of a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined in claims 1 to 3,

- at least an anti-tumoral agent, for the manufacture of a combination product for a simultaneous, separate, or spread over time use for the prevention or the treatment of primary tumors or invasive or metastasic tumors,

or of a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined in claims 1 to 3,

- at least an anti-angiogenic agent,

- at least an anti-tumoral agent,

for the manufacture of a combination product for a simultaneous, separate, or spread over time use for the prevention or the treatment of primary tumors or invasive or metastasic tumors.

5. Use according to any one of claims 1 to 4, wherein the drug is combined with an anti- tumoral therapy, such as radiotherapy or a chemotherapy.

6. Use according to any one of claims 1 to 5, wherein the primary tumor or the invasive or metastatic tumor is a solid tumor or a lymphoproliferative tumor, wherein the solid tumor is prostate tumor, a liver tumor, hepatic adenomas, focal nodular hyperplasia, a brain tumor such as glioma, a breast tumor, a kidney tumor, a lung tumor such as non- small cell lung carcinoma, small cell lung carcinoma, pleuropulmonary blastoma and carcinoid tumor, a bone tumor such as osteoma, osteochondroma, aneurysmal bone cyst, and fibrous dysplasia, osteosarcoma, chondrosarcoma, Ewing's sarcoma, malignant fibrous histiocytoma, fibrosarcoma, a stomach cancer, a colon tumor, a small bowel tumor, a esophageal tumor, a pancreatic tumor, a sarcoma, a cervical tumor, a gall blader tumor, a melanoma, and wherein lymphoproliferative tumor is Leukemia, Lymphoma, or a Multiple myeloma.

7. Pharmaceutical composition, comprising as active substance, at least

- one inhibitor of protein expression, which inhibits the expression of:

- TSPl protein, or

- a protein, which controls the expression of TSPl or mediates the activity of

TSPl or,

- one inhibitor of protein activity, said inhibitor inhibiting the activity of:

- the TSPl protein, in particular the activity responsible for the stimulation of cell migration, or - one protein which controls the expression or mediates the activity of TSPl, in association with a pharmaceutically acceptable vehicle.

8. Pharmaceutical composition according to claim 7, wherein the inhibitor of protein expression inhibits the expression of proteins chosen from the group comprising

TSPl, TRPV2, TRPV3, TRPV6, TRPM8, CD36, or B3 integrin.

9. Pharmaceutical composition according to claims 7 or 8, wherein the inhibitor of the protein expression is a double-stranded oligonucleotide or a single-stranded oligonucleotide.

10. Pharmaceutical composition according to any one of claims 7 to 9, wherein the inhibitor of the protein expression is:

wherein the oligonucleotide sequence (a)

- is either complementary to the oligonucleotide sequence (b),

wherein the oligonucleotide sequence (a)

- or a fragment of the above-defined double-stranded oligonucleotides (a) and (b), comprising two complementary fragments of the respective above-defined oligonucleotide sequences (a) and (b), provided that said fragment conserves the property of inhibiting the expression of one of the proteins above-defined,

- or a double-stranded oligonucleotide comprising two oligonucleotide sequences, (a) and (b), forming a hybrid, wherein each oligonucleotide sequence comprises at one of its 3 ' or 5 ' ends, one to five unpaired nucleotides forming single- stranded ends extending beyond the hybrid, wherein the part inside the hybrid of the oligonucleotide sequence (a)

- is either complementary to the oligonucleotide sequence (b),

wherein the oligonucleotide sequence (a)

11. Pharmaceutical composition according to any one of claims 7 to 10, wherein the oligonucleotide sequence complementary to the target sequence comprises from 15 to 25 nucleotides.

12. Pharmaceutical composition according to any one of claims 7 to 11, wherein the double-stranded oligonucleotide comprises, at the 3' end of each of the two said oligonucleotide sequences, 1 to 5 nucleotides, preferably 2 to 3 nucleotides, extending beyond the hybrid, in particular wherein the nucleotides extending beyond the hybrid are deoxythymidines

13. Pharmaceutical composition according to any one of claims 7 to 12, wherein the target sequence is represented by SEQ ID NO: 41(TSPl) or SEQ ID NO: 42(TRPV3).

14. Pharmaceutical composition according to any one of claims 7 to 13, wherein the double-stranded oligonucleotide is chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 9 and SEQ ID NO: 10) ; (SEQ ID NO: 11 and SEQ ID NO: 12); (SEQ ID NO: 13 and SEQ ID NO: 14); (SEQ ID NO: 15 and SEQ ID NO: 16); (SEQ ID NO: 17 and SEQ ID NO: 18); (SEQ ID NO: 19 and SEQ ID NO: 20); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 29 and SEQ ID NO: 30); (SEQ

ID NO: 31 and SEQ ID NO: 32); (SEQ ID NO: 33 and SEQ ID NO: 34); (SEQ ID NO: 35 and SEQ ID NO: 36); (SEQ ID NO: 37 and SEQ ID NO: 38); (SEQ ID NO: 39 and SEQ ID NO: 40). (see table of sequences) 15. Pharmaceutical composition according to any one of claims 7 to 14, wherein the active substance is formulated for the administration at a dose in the range of 0,05 to 50 mg/kg, in particular 0,1 to 20 mg/kg.

16. Pharmaceutical composition comprising, as active substance, a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined in claims 1 to 3,

- at least an anti-angiogenic agent,

as combination product for a simultaneous, separate, or spread over time use, or

a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined in claims 1 to 3,

- at least an anti-tumoral agent,

a product containing or consisting in:

- at least a double-stranded oligonucleotide above-defined in claims 1 to 3,

- at least an anti-angiogenic agent,

- at least an anti-tumoral agent,

as combination product for a simultaneous, separate, or spread over time use.

17. Pharmaceutical composition according to claim 16, wherein the anti-angiogenic agent is chosen from the group comprising Cilengitide, Vandetanib, Lenalidomide, Thalidomide, Arsenic Trioxide, Bevacizumab, anti-VEGFR-1, anti-VEGFR-2, anti- PDGFR, anti-FMS-FLT-3, anti-TKl.

18. Pharmaceutical composition according to claims 16 or 17, wherein the anti-tumoral agent is chosen from the group comprising alkylating agents, such as Bendamustine, Temozolomide, Mechlorethamine, Cyclophosphamide, Carmustine, Cisplatine, Busulfan, Thiotepa, or Decarbazine, anti-metabolite agents, such as Pentostatine, Methotrexate, Pemetrexed, Floxuridine, Fluorouracil, Cytaraine, Mercaptopurine or

Thiguanine, cytotoxic antibiotics such as Rubitecan, Mitomycine C, Daunorubicin, Doxorubicine, Bleomycin, Plicamycin, Mitoxantrone HCl, or Oxaliplatine, plant derivatives, such as Vinorelbine, BMS 184476, Vincristine sulfate, Vinblastine, Docetaxel taxol.

19. Oligonucleotide sequence chosen from the following sequences: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID

NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40.

20. Double-stranded oligonucleotides chosen from the following couples consisting in (SEQ ID NO: 1 and SEQ ID NO: 2);(SEQ ID NO: 3 and SEQ ID NO: 4); (SEQ ID NO: 5 and SEQ ID NO: 6),; (SEQ ID NO: 7 and SEQ ID NO: 8); (SEQ ID NO: 9 and SEQ ID NO: 10) ; (SEQ ID NO: 11 and SEQ ID NO: 12); (SEQ ID NO: 13 and SEQ ID NO: 14); (SEQ ID NO: 15 and SEQ ID NO: 16); (SEQ ID NO: 17 and SEQ ID

NO: 18); (SEQ ID NO: 19 and SEQ ID NO: 20); (SEQ ID NO: 21 and SEQ ID NO: 22); (SEQ ID NO: 23 and SEQ ID NO: 24); (SEQ ID NO: 25 and SEQ ID NO: 26); (SEQ ID NO: 27 and SEQ ID NO: 28); (SEQ ID NO: 29 and SEQ ID NO: 30); (SEQ ID NO: 31 and SEQ ID NO: 32); (SEQ ID NO: 33 and SEQ ID NO: 34); (SEQ ID NO: 35 and SEQ ID NO: 36); (SEQ ID NO: 37 and SEQ ID NO: 38); (SEQ ID NO:

39 and SEQ ID NO: 40).