WO2011012091A1 - An enzymolysis device - Google Patents

An enzymolysis device Download PDF

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Publication number
WO2011012091A1
WO2011012091A1 PCT/CN2010/075600 CN2010075600W WO2011012091A1 WO 2011012091 A1 WO2011012091 A1 WO 2011012091A1 CN 2010075600 W CN2010075600 W CN 2010075600W WO 2011012091 A1 WO2011012091 A1 WO 2011012091A1
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WO
WIPO (PCT)
Prior art keywords
flash column
interface
enzymolysis
communicated
communicating vessel
Prior art date
Application number
PCT/CN2010/075600
Other languages
French (fr)
Inventor
Guojun Yue
Likang Deng
Xiaoming Hao
Tianyang Yu
Hailong Lin
Xuexian Chen
Li Wang
Original Assignee
Cofco Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cofco Limited filed Critical Cofco Limited
Publication of WO2011012091A1 publication Critical patent/WO2011012091A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes

Definitions

  • the present invention relates to an enzymolysis device.
  • the enzymolysis is generally performed in an enzymolysis tank.
  • the material to be enzyme-digested is mixed with zymogenic microbes grow and/or enzyme in the enzymolysis tank.
  • the conditions for enzymolysis include enzymolysis temperature, time and pH value, wherein enzymolysis temperature is generally the temperature at which zymogenic microbes grow and/or enzyme is active, so during enzymolysis, the enzymolysis tank usually needs to be heated to enzymolysis temperature.
  • an insulated heater is installed at the bottom of the enzymolysis tank, the heater is turned on before enzymolysis to preheat the enzymolysis tank, and after enzymolysis temperature is reached, the material to be enzyme-digested and zymogenic microbes and/or enzyme will be added into the enzymolysis tank to perform enzymolysis.
  • the pipeline is easy to be blocked.
  • the object of the present invention is to overcome problem of the existing enzymolysis device that the pipeline is easy to be blocked, and an enzymolysis device which can avoide the blockage of the pipeline during enzymolysis.
  • the present invention provides an enzymolysis device, comprising:
  • a flash column comprising a first interface, a second interface, a third interface and an outlet
  • a heat source communicated with the third interface of the flash column via a communicating vessel , the top of the communicating vessel being higher than the liquid level of the material to be enzyme-digested in the flash column;
  • a material source communicated with the flash column via the first interface; and a vacuum pump communicated with the second interface of the flash column.
  • the inventors of the present invention tactfully communicate heat source with the third interface of the flash column, even if the vacuum degree in the flash column doesn't meet the condition of sucking in the heat medium owing to the instability of the vacuum pump during work or irregular operation of the vacuum pump, as the top of the communicating vessel is higher than the top of the flash column, the pressure is not enough to reversely suck the material to be enzyme-digested into the pipeline that links the flash column and the heat source, thereby avoiding the blockage of the pipeline and improving enzymolysis efficiency.
  • both the heat source and the material source are communicated with the flash column so as to make the heat source and the material source exchange heat in the flash column, such that steam generated in other workshop sections is used as a heat source to heat the material and thus the efficiency of enzymolysis is greatly improved.
  • FIG. 1 is a structural schematic of the enzymolysis device provided by the present invention.
  • the enzymolysis device As shown in FIG. 1, the enzymolysis device provided by the present invention comprises:
  • a flash column 1 comprising a first interface 5, a second interface 6, a third interface 7 and an outlet 11 ;
  • a heat source 2 communicated with the third interface 7 of the flash column 1 via a communicating vessel 8, the top of the communicating vessel 8 being higher than the liquid level of the material to be enzyme-digested in the flash column 1;
  • a vacuum pump 4 communicated with the second interface 6 of the flash column 1.
  • the enzymolysis tank 3 is communicated with the outlet of the flash column 1, the vacuum pump 4 is communicated with the second interface 6 of the flash column 1, and the heat source 2 is communicated with the third interface 7 of the flash column 1.
  • the temperature of the heat medium in the heat source 2 may be around 130 ° C .
  • the vacuum pump 4 may be turned on before, during or after the material to be enzyme-digested is delivered from the material source 10 into the flash column 1, so that the flash column 1 is vacuumized. When the vacuum degree of the flash column 1 reaches a specific value, the heat medium can be sucked from the heat source 2 into the flash column 1.
  • the material to be enzyme-digested is delivered into the flash column 1 from the material source 10 via the first interface 5, the material to be enzyme-digested contacts the heat medium in the flash column 1 to complete heat exchange and raise the temperature of the material to be enzyme-digested.
  • the temperature of the material to be enzyme-digested reaches enzymolysis temperature, the material will be directly fed into the enzymolysis tank 3 to undergo enzymolysis.
  • the pressure in the flash column 1 is not enough to reversely suck the pulverized product into the pipeline that links the flash column 1 and the heat source 2, while owing to the gravity of the material, the pulverized product reversely sucked into the communicating vessel 8 will flow back to the flash column 1 before it reaches the top of the communicating vessel, thereby avoiding the reverse suction of the material into the pipeline and the blockage of the pipeline.
  • the top of the communicating vessel 8 is higher than the top of the flash column 1 and the height difference between the top of the communicating vessel 8 and the top of the flash column 1 may be l-2.5m, and more preferably 1.5 -2m.
  • the preferred communicating vessel 8 is a bent tube, for example, the bent tube may be an inverted U-tube or serpentine tube.
  • the more preferred communicating vessel 8 is an inverted U-tube, and the height difference between the top of the inverted U-tube and the top of the flash column 1 may be l-2.5m, and preferably 1.5-2m.
  • the communicating vessel 8 may be made from a material with a strength and resistant to heat, for example, iron or stainless steel.
  • the present invention in order to facilitate better heating of the material to be enzyme-digested by hot steam, it is preferred to countercurrently contact the heat medium from the heat source 2 with the material to be enzyme-digested in the flash column 1, i.e. make the position of the first interface 5 through which the pulverized product is input lower than the position the third interface 7 which is communicated with the heat source 2 through the communicating vessel 8.
  • valves are installed at any one or more of the following positions: a valve may be installed between the communicating vessel 8 and the third interface 7 of the flash column 1 ; a valve may be installed between the communicating vessel 8 and the heat source 2; and a valve may be installed between the material source 10 and the first interface 5.
  • the flash column 1 may be a conventional flash column in the art.
  • it may be any kind of the commonly used packed column or sieve -plate column.
  • the number or theoretical number of the trays of the flash column 1 depends on the expected heat exchange degree. Usually, when other conditions are same, the larger the number or theoretical number of the trays is, the higher the heat exchange degree will be, i.e. the more sufficiently the heat energy of the heat medium will be transferred to the material to be enzyme-digested.
  • the inventors of the present invention discovered from research, when the pulverized product is 20-40 ° C starch slurry made from root and tuber crops, and the heat medium is 100-170 ° C steam, the preferred number or theoretical number of the trays of the flash column 1 is 2-6, and under this condition, the temperature of the material to be enzyme-digested discharged from the flash column 1 may be controlled at 50-90 ° C which meets the requirement of enzymo lysis.
  • the packed column may be packed with one or more of Rasching ring, Pall ring, cascade mini ring, saddle ring, Berl saddle ring, Intalox saddle ring, Dixon ring, Cannon Ring, structured Mellpak packing and structured gauze packing.
  • the sieve plates of the sieve-plate column are provided with overflow weirs, so that the heat medium passes through the sieve pores of the sieve plates from the bottom of the sieve-plate column and flows upwards, and the pulverized product flows downwards and enters next sieve plate when it stays on a sieve plate and reaches the height of the overflow weir.
  • the first interface 5 is arranged at tray 0 or tray 1 of the packed column or sieve-plate column
  • the third interface 7 is arranged at the last tray of the packed column or sieve-plate column or a position closer to column bottom.
  • a temperature test unit may also be installed on the flash column 1, to real-time monitor the temperature of the material to be enzyme-digested in the flash column 1.
  • the material to be enzyme-digested may be delivered to the enzymolysis tank 3 for enzymolysis.
  • a liquid level test unit may also be installed on the flash column 1 , to monitor the liquid level of the material to be enzyme-digested delivered into the flash column 1.
  • the gauge pressure of the flash column 1 may be -0.3 MPa to -0.01 Mpa and the preferred value is -0.1 MPa to -0.05 Mpa; the weight ratio of the material to be enzyme-digested to the heat medium that contact each other in the flash tower 1 is 15-30:1; the contact time should guarantee that the material to be enzyme-digested can reach enzymolysis temperature at least. Normally, the contact time may be 5-10min.
  • the heat source 2 may provide various heat medium such as steam, hot water and so on.
  • the heat source 2 may be a pipeline that may convey various heat medium, and alternatively, it may be a container storing various heat medium.
  • the heat source 2 is preferably a heat medium generated in other workshop sections, such as the waste steam and hot water discharged from the rectification section.
  • the heat source 2 is preferably a container capable of storing various heat medium so that the heat medium may be temporarily stored in the container before contact, and the temperature of the heat medium is typically 100-170 ° C .
  • the enzymolysis tank may be any conventional enzymolysis tank in the art, such as a 250m 3 carbon steel container provided with a stirring device.
  • a temperature test unit may be installed on the enzymolysis tank 3.
  • the position of the second interface 6 that links the vacuum pump 4 and the flash column 1 is not particularly limited, it may be in any position of the flash column 1 , and the preferred position is the middle or middle upper part of the flash column 1.
  • this device may also comprise a condenser 9 which may be communicated with the upper part of the flash column 1 so that the steam in the flash column 1 is sent to the condenser 9 after it contacts the material to be enzyme-digested and in the condenser 9, it is condensed into water to be used in other workshop sections.
  • the enzymo lysis device provided in the method of the present invention also comprises the condenser 9 and the heat medium adopted is hot steam or hot water
  • the enzymolysis in the enzymolysis device provided in the method of the present invention can also generate byproduct distilled water in the same time.
  • the condenser 9 is connected to the top of the flash column 1.
  • the condenser may be any conventional condenser in the art, such as: shell and tube condenser.
  • the inventors of the present invention discovered from research that, when the material to be enzyme-digested is 20-40 ° C starch slurry preferably made from root and tuber crops, and the heat medium is 100-170 ° C steam, the preferred number or theoretical number of the trays of the flash column 1 is 2-6, and under this condition, the temperature of the material to be enzyme-digested discharged from the flash column 1 may be controlled at 50-90 ° C which meets the requirement of enzymolysis.
  • the present invention will be described more detailedly with reference to Fig. 1.
  • This example intends to describe the enzymolysis method using the enzymolysis device provided by the present invention.
  • the enzymolysis device shown in FIG. 1 is used to perform enzymolysis.
  • the enzymolysis device comprises a flash column 1, a heat source 2, an enzymolysis tank 3, a material source 10, a vacuum pump 4 and a condenser 9.
  • the flash column 1 comprises a first interface 5, a second interface 6, a third interface 7 and at least one outlet.
  • the material source 10 is communicated with the flash column 1 via the first interface 5, the enzymolysis tank 3 is communicated with the outlet of the flash column 1 , the vacuum pump 4 is communicated with the second interface 6 of the flash column 1 , the condenser 9 is communicated with the top of the flash column 1 , the heat source 2 is communicated with the third interface 7 through an inverted U-tube, the top of the U-tube is higher than the top of the flash column 1, and the height difference between the inverted U-tube and the top of the flash column 1 is 2.5m.
  • the number of trays of the flash column is 6, and from top to bottom, the first interface 5 and the third interface 7 are at tray 1 and tray 6 of the flash column, respectively.
  • the heat medium in the heat source 2 is 150 ° C steam, and the material to be enzyme-digested of the material source 10 is 25 ° C 80wt% starch slurry.
  • the vacuum pump 4 is turned on to vacuum the flash column 1 and ensure the gauge pressure of the flash column 1 is -O.lMpa. Then the valve between the heat source 2 and the flash column 1 is opened so that the 150 ° C steam in the heat source 2 is sucked into the flash column 1. Meanwhile, the valve of the material source 10 is opened so that the 20 ° C starch slurry is delivered from the material source 10 to the flash column 1 via the first interface 5 and the starch slurry and steam contact each other in the flash column 1. The weight ratio of steam to starch slurry is 25:1. The contact time is 5min. The temperature of the starch slurry is monitored by a temperature monitoring device installed on the flash column 1.
  • the starch slurry When the temperature is raised to 55 ° C, the starch slurry will be delivered to the enzymo lysis tank 3 through the outlet. In the tank, the starch slurry is mixed with amylase and undergoes enzymolysis.
  • the enzymolysis time is 60min and the pH value for the enzymolysis is 5; based on Ig of the dry weight of dry pulverized product, 20 enzyme-activity units of ⁇ -amylase (purchased from Novozymes) is added; and the condenser 9 is turned on to draw out the remaining steam from the flash column 1 and condense it into water.

Abstract

The present invention provides an enzymolysis device, comprising: a flash column (1) comprising a first interface (5), a second interface (6), a third interface (7) and an outlet (11); a heat source (2) communicated with the third interface (7) of the flash 5 column (1) via a communicating vessel (8), the top of the communicating vessel (8) being higher than the liquid level of the material to be enzyme-digested in the flash column (1); an enzymolysis tank (3) communicated with the outlet of the flash column (1); a material source (10) communicated with the flash column (1) via the first interface (5); and a vacuum pump (4) communicated with the second interface (6) 10 of the flash column (1).

Description

An enzymolysis device
Field of the Invention
The present invention relates to an enzymolysis device.
Background of the Invention
The enzymolysis is generally performed in an enzymolysis tank. For example, the material to be enzyme-digested is mixed with zymogenic microbes grow and/or enzyme in the enzymolysis tank. The conditions for enzymolysis include enzymolysis temperature, time and pH value, wherein enzymolysis temperature is generally the temperature at which zymogenic microbes grow and/or enzyme is active, so during enzymolysis, the enzymolysis tank usually needs to be heated to enzymolysis temperature. Most commonly, an insulated heater is installed at the bottom of the enzymolysis tank, the heater is turned on before enzymolysis to preheat the enzymolysis tank, and after enzymolysis temperature is reached, the material to be enzyme-digested and zymogenic microbes and/or enzyme will be added into the enzymolysis tank to perform enzymolysis. During enzymolysis in an existing enzymolysis device, the pipeline is easy to be blocked. Summary of the Invention
The object of the present invention is to overcome problem of the existing enzymolysis device that the pipeline is easy to be blocked, and an enzymolysis device which can avoide the blockage of the pipeline during enzymolysis.
The present invention provides an enzymolysis device, comprising:
a flash column comprising a first interface, a second interface, a third interface and an outlet;
a heat source communicated with the third interface of the flash column via a communicating vessel , the top of the communicating vessel being higher than the liquid level of the material to be enzyme-digested in the flash column;
an enzymolysis tank communicated with the outlet of the flash column;
a material source communicated with the flash column via the first interface; and a vacuum pump communicated with the second interface of the flash column.
The inventors of the present invention tactfully communicate heat source with the third interface of the flash column, even if the vacuum degree in the flash column doesn't meet the condition of sucking in the heat medium owing to the instability of the vacuum pump during work or irregular operation of the vacuum pump, as the top of the communicating vessel is higher than the top of the flash column, the pressure is not enough to reversely suck the material to be enzyme-digested into the pipeline that links the flash column and the heat source, thereby avoiding the blockage of the pipeline and improving enzymolysis efficiency. In addition, both the heat source and the material source are communicated with the flash column so as to make the heat source and the material source exchange heat in the flash column, such that steam generated in other workshop sections is used as a heat source to heat the material and thus the efficiency of enzymolysis is greatly improved. Brief Description of the Drawings
FIG. 1 is a structural schematic of the enzymolysis device provided by the present invention.
Detailed Description of the Embodiments
As shown in FIG. 1, the enzymolysis device provided by the present invention comprises:
a flash column 1 comprising a first interface 5, a second interface 6, a third interface 7 and an outlet 11 ;
a heat source 2 communicated with the third interface 7 of the flash column 1 via a communicating vessel 8, the top of the communicating vessel 8 being higher than the liquid level of the material to be enzyme-digested in the flash column 1;
an enzymolysis tank 3 communicated with the outlet of the flash column 1;
a material source 10 communicated with the flash column 1 via the first interface 5; and
a vacuum pump 4 communicated with the second interface 6 of the flash column 1.
According to the method provided by the present invention, the enzymolysis tank 3 is communicated with the outlet of the flash column 1, the vacuum pump 4 is communicated with the second interface 6 of the flash column 1, and the heat source 2 is communicated with the third interface 7 of the flash column 1. The temperature of the heat medium in the heat source 2 may be around 130°C . The vacuum pump 4 may be turned on before, during or after the material to be enzyme-digested is delivered from the material source 10 into the flash column 1, so that the flash column 1 is vacuumized. When the vacuum degree of the flash column 1 reaches a specific value, the heat medium can be sucked from the heat source 2 into the flash column 1. The material to be enzyme-digested is delivered into the flash column 1 from the material source 10 via the first interface 5, the material to be enzyme-digested contacts the heat medium in the flash column 1 to complete heat exchange and raise the temperature of the material to be enzyme-digested. When the temperature of the material to be enzyme-digested reaches enzymolysis temperature, the material will be directly fed into the enzymolysis tank 3 to undergo enzymolysis.
Owing to the instability of the vacuum pump during work or irregular operation of the vacuum pump, when the vacuum degree in the flash column 1 doesn't meet the condition of sucking in the heat medium, there will be a tendency that the pulverized product in the flash column 1 is reversely sucked into the communicating vessel 8, and if the top of the communicating vessel 8 is lower than or at equal height with the liquid level of the pulverized product in the flash column 1 , the pulverized product in the flash column 1 will be sucked into the communicating vessel 8, thereby blocking the pipeline. According to the enzymolysis device provided by the present invention, as the top of the communicating vessel 8 is higher than the liquid level of the pulverized product in the flash column 1 and the pressure in the flash column 1 is lower than that in the heat source 2, the pressure in the flash column 1 is not enough to reversely suck the pulverized product into the pipeline that links the flash column 1 and the heat source 2, while owing to the gravity of the material, the pulverized product reversely sucked into the communicating vessel 8 will flow back to the flash column 1 before it reaches the top of the communicating vessel, thereby avoiding the reverse suction of the material into the pipeline and the blockage of the pipeline.
According to the present invention, preferably, for convenient, the top of the communicating vessel 8 is higher than the top of the flash column 1 and the height difference between the top of the communicating vessel 8 and the top of the flash column 1 may be l-2.5m, and more preferably 1.5 -2m. As bend-type communicating vessel hardly forms a dead corner and can make material flow more smoothly, the preferred communicating vessel 8 is a bent tube, for example, the bent tube may be an inverted U-tube or serpentine tube. In consideration of cost, according to a specific embodiment of the present invention, the more preferred communicating vessel 8 is an inverted U-tube, and the height difference between the top of the inverted U-tube and the top of the flash column 1 may be l-2.5m, and preferably 1.5-2m.
The communicating vessel 8 may be made from a material with a strength and resistant to heat, for example, iron or stainless steel.
According to the present invention, in order to facilitate better heating of the material to be enzyme-digested by hot steam, it is preferred to countercurrently contact the heat medium from the heat source 2 with the material to be enzyme-digested in the flash column 1, i.e. make the position of the first interface 5 through which the pulverized product is input lower than the position the third interface 7 which is communicated with the heat source 2 through the communicating vessel 8.
In order to more easily control the amount of the hot steam that contacts the material to be enzyme-digested so as to control the temperature of the material to be enzyme-digested, and in order to easily control the input amount of the material to be enzyme-digested so as to control the liquid level of the material to be enzyme-digested in the flash column, preferably, valves are installed at any one or more of the following positions: a valve may be installed between the communicating vessel 8 and the third interface 7 of the flash column 1 ; a valve may be installed between the communicating vessel 8 and the heat source 2; and a valve may be installed between the material source 10 and the first interface 5.
According to the present invention, the flash column 1 may be a conventional flash column in the art. For example, it may be any kind of the commonly used packed column or sieve -plate column. The number or theoretical number of the trays of the flash column 1 depends on the expected heat exchange degree. Usually, when other conditions are same, the larger the number or theoretical number of the trays is, the higher the heat exchange degree will be, i.e. the more sufficiently the heat energy of the heat medium will be transferred to the material to be enzyme-digested. The inventors of the present invention discovered from research, when the pulverized product is 20-40 °C starch slurry made from root and tuber crops, and the heat medium is 100-170°C steam, the preferred number or theoretical number of the trays of the flash column 1 is 2-6, and under this condition, the temperature of the material to be enzyme-digested discharged from the flash column 1 may be controlled at 50-90 °C which meets the requirement of enzymo lysis.
The packed column may be packed with one or more of Rasching ring, Pall ring, cascade mini ring, saddle ring, Berl saddle ring, Intalox saddle ring, Dixon ring, Cannon Ring, structured Mellpak packing and structured gauze packing. Preferably, the sieve plates of the sieve-plate column are provided with overflow weirs, so that the heat medium passes through the sieve pores of the sieve plates from the bottom of the sieve-plate column and flows upwards, and the pulverized product flows downwards and enters next sieve plate when it stays on a sieve plate and reaches the height of the overflow weir. In order to further increase heat exchange efficiency, the first interface 5 is arranged at tray 0 or tray 1 of the packed column or sieve-plate column, and the third interface 7 is arranged at the last tray of the packed column or sieve-plate column or a position closer to column bottom.
According to the present invention, a temperature test unit may also be installed on the flash column 1, to real-time monitor the temperature of the material to be enzyme-digested in the flash column 1. When the temperature of the material to be enzyme-digested in the flash column 1 reaches the enzymolysis condition, the material to be enzyme-digested may be delivered to the enzymolysis tank 3 for enzymolysis. Further, a liquid level test unit may also be installed on the flash column 1 , to monitor the liquid level of the material to be enzyme-digested delivered into the flash column 1.
According to the present invention, the gauge pressure of the flash column 1 may be -0.3 MPa to -0.01 Mpa and the preferred value is -0.1 MPa to -0.05 Mpa; the weight ratio of the material to be enzyme-digested to the heat medium that contact each other in the flash tower 1 is 15-30:1; the contact time should guarantee that the material to be enzyme-digested can reach enzymolysis temperature at least. Normally, the contact time may be 5-10min.
According to the present invention, the heat source 2 may provide various heat medium such as steam, hot water and so on. For instance, the heat source 2 may be a pipeline that may convey various heat medium, and alternatively, it may be a container storing various heat medium.
In order to save and reuse energy, the heat source 2 is preferably a heat medium generated in other workshop sections, such as the waste steam and hot water discharged from the rectification section.
When the heat medium in the heat source 2 contacts the material to be enzyme-digested in the flash column 1 , in order to guarantee the amount of the heat medium, the heat source 2 is preferably a container capable of storing various heat medium so that the heat medium may be temporarily stored in the container before contact, and the temperature of the heat medium is typically 100-170°C .
The enzymolysis tank may be any conventional enzymolysis tank in the art, such as a 250m3 carbon steel container provided with a stirring device. In order to monitor enzymolysis temperature, a temperature test unit may be installed on the enzymolysis tank 3.
There may be one or more vacuum pumps 4 in parallel as long as the flash column 1 can meet the requirement of vacuum degree. The position of the second interface 6 that links the vacuum pump 4 and the flash column 1 is not particularly limited, it may be in any position of the flash column 1 , and the preferred position is the middle or middle upper part of the flash column 1.
After the heat medium provided by the heat source 2 exchanges heat with the material to be enzyme-digested in the flash column 1 in form of steam, the remaining hot steam may be directly discharged out of the flash column 1. In order to meet the requirements of environmental protection, this device may also comprise a condenser 9 which may be communicated with the upper part of the flash column 1 so that the steam in the flash column 1 is sent to the condenser 9 after it contacts the material to be enzyme-digested and in the condenser 9, it is condensed into water to be used in other workshop sections. Therefore, when the enzymo lysis device provided in the method of the present invention also comprises the condenser 9 and the heat medium adopted is hot steam or hot water, the enzymolysis in the enzymolysis device provided in the method of the present invention can also generate byproduct distilled water in the same time. Preferably, for easy operation, the condenser 9 is connected to the top of the flash column 1. The condenser may be any conventional condenser in the art, such as: shell and tube condenser.
The inventors of the present invention discovered from research that, when the material to be enzyme-digested is 20-40 °C starch slurry preferably made from root and tuber crops, and the heat medium is 100-170°C steam, the preferred number or theoretical number of the trays of the flash column 1 is 2-6, and under this condition, the temperature of the material to be enzyme-digested discharged from the flash column 1 may be controlled at 50-90 °C which meets the requirement of enzymolysis. Hereinafter the present invention will be described more detailedly with reference to Fig. 1.
Example 1
This example intends to describe the enzymolysis method using the enzymolysis device provided by the present invention.
The enzymolysis device shown in FIG. 1 is used to perform enzymolysis.
The enzymolysis device comprises a flash column 1, a heat source 2, an enzymolysis tank 3, a material source 10, a vacuum pump 4 and a condenser 9. The flash column 1 comprises a first interface 5, a second interface 6, a third interface 7 and at least one outlet. The material source 10 is communicated with the flash column 1 via the first interface 5, the enzymolysis tank 3 is communicated with the outlet of the flash column 1 , the vacuum pump 4 is communicated with the second interface 6 of the flash column 1 , the condenser 9 is communicated with the top of the flash column 1 , the heat source 2 is communicated with the third interface 7 through an inverted U-tube, the top of the U-tube is higher than the top of the flash column 1, and the height difference between the inverted U-tube and the top of the flash column 1 is 2.5m. The number of trays of the flash column is 6, and from top to bottom, the first interface 5 and the third interface 7 are at tray 1 and tray 6 of the flash column, respectively. The heat medium in the heat source 2 is 150°C steam, and the material to be enzyme-digested of the material source 10 is 25 °C 80wt% starch slurry.
The vacuum pump 4 is turned on to vacuum the flash column 1 and ensure the gauge pressure of the flash column 1 is -O.lMpa. Then the valve between the heat source 2 and the flash column 1 is opened so that the 150°C steam in the heat source 2 is sucked into the flash column 1. Meanwhile, the valve of the material source 10 is opened so that the 20 °C starch slurry is delivered from the material source 10 to the flash column 1 via the first interface 5 and the starch slurry and steam contact each other in the flash column 1. The weight ratio of steam to starch slurry is 25:1. The contact time is 5min. The temperature of the starch slurry is monitored by a temperature monitoring device installed on the flash column 1. When the temperature is raised to 55°C, the starch slurry will be delivered to the enzymo lysis tank 3 through the outlet. In the tank, the starch slurry is mixed with amylase and undergoes enzymolysis. The enzymolysis time is 60min and the pH value for the enzymolysis is 5; based on Ig of the dry weight of dry pulverized product, 20 enzyme-activity units of α-amylase (purchased from Novozymes) is added; and the condenser 9 is turned on to draw out the remaining steam from the flash column 1 and condense it into water.

Claims

Claims
What is claimed is: 1. An enzymo lysis device, comprising:
a flash column (1) comprising a first interface (5), a second interface (6), a third interface (7) and an outlet (11);
a heat source (2) communicated with the third interface (7) of the flash column (1) via a communicating vessel (8), the top of the communicating vessel (8) being higher than the liquid level of the material to be enzyme-digested in the flash column (1); an enzymo lysis tank (3) communicated with the outlet of the flash column (1);
a material source (10) communicated with the flash column (1) via the first interface
(5); and
a vacuum pump (4) communicated with the second interface (6) of the flash column (1).
2. The device according to claim 1, wherein the top of the communicating vessel (8) is higher than the top of the flash column (1).
3. The device according to claim 2, wherein the height difference between the top of the communicating vessel (8) and the top of the flash column (1) is l-2.5m.
4. The device according to any of claims 1-3, wherein the communicating vessel (8) is a bent tube.
5. The device according to claim 4, wherein the communicating vessel (8) is an inverted U-tube or serpentine tube.
6. The device according to claim 1, wherein the heat medium is countercurrently contacted with the pulverized product.
7. The device according to claim 1, wherein a valve is installed at any one or more of the following positions: between the communicating vessel (8) and the third interface (7) of the flash column (1); between the communicating vessel (8) and the heat source (2); and between the material source (10) and the first interface (5).
8. The device according to claim 1, 3 or 7, wherein the flash column (1) is a packed column or sieve-plate column.
9. The device according to claim 1, wherein the enzymolysis tank (3) is a container with a stirrer.
10. The device according to claim 1 or 7, wherein the device further comprises a condenser (9) communicated with the upper part of the flash column (1).
11. The device according to claim 10, wherein the condenser (9) is communicated with the top of the flash column (1).
PCT/CN2010/075600 2009-07-31 2010-07-30 An enzymolysis device WO2011012091A1 (en)

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CN113136327A (en) * 2021-03-16 2021-07-20 南通职业大学 Real silk hydrophobic reaction device of high-efficient catalysis of biological enzyme

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CN106380527A (en) * 2016-11-15 2017-02-08 如皋市坝新肠衣有限公司 Device for preparing heparin sodium by concentrating multilevel special resin

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CN112772817A (en) * 2021-01-30 2021-05-11 乳山中诚果汁饮料有限公司 Concentrated fruit juice production line and process flow thereof
CN113136327A (en) * 2021-03-16 2021-07-20 南通职业大学 Real silk hydrophobic reaction device of high-efficient catalysis of biological enzyme

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