WO2011006508A2 - Adhesive patch with zonase - Google Patents
Adhesive patch with zonase Download PDFInfo
- Publication number
- WO2011006508A2 WO2011006508A2 PCT/DK2010/050188 DK2010050188W WO2011006508A2 WO 2011006508 A2 WO2011006508 A2 WO 2011006508A2 DK 2010050188 W DK2010050188 W DK 2010050188W WO 2011006508 A2 WO2011006508 A2 WO 2011006508A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- patch
- skin
- enzyme
- adhesive
- tissue
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
- A61K9/703—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms characterised by shape or structure; Details concerning release liner or backing; Refillable patches; User-activated patches
- A61K9/7084—Transdermal patches having a drug layer or reservoir, and one or more separate drug-free skin-adhesive layers, e.g. between drug reservoir and skin, or surrounding the drug reservoir; Liquid-filled reservoir patches
Definitions
- the invention relates to an adhesive patch for application to callous skin and corns and to a method of removing or thinning keratinaceous tissue.
- Hyperkeratosis or thickening of the stratum corneum, is a widespread condition that can either be a symptom of a certain pathological condition, such as psoriasis, arsenic poisoning, certain forms of dermatitis, ichthyosis or it may simply be caused by excessive wear of an area of the skin, callus. In both cases, the problem is often local and may therefore be treated with an occlusive patch comprising an active chemical to aid the degradation of keratin.
- Other common methods for managing hyperkeratinized tissue are the application of acids in lotion or cream carriers, moisturizing creams or lotions or mechanical scraping.
- acids and scraping are disadvantageous because they tend to irritate normal epidermis and moisturizing creams are not effective in reducing hyperkeratotic tissue density.
- Non-occlusive solutions comprise the usage of stronger acids, such as trichloroacetic acid, or surgical removal.
- Proteolytic enzymes may be used for degrading the hyperkeratotic tissue.
- US patent no. 5,213,978 and US patent no. 5,395,613 disclose the use of proteases derived from the bacterium Micrococcus sedentarius
- US patent no. 6,858,215 disclose the use of subtilisin and fungal proteases for callous tissue.
- the invention relates to an adhesive patch for the removal or thinning of keratinaceous tissue, the patch comprising a first skin-facing surface and a second non-skin-facing surface, the patch comprises an adhesive layer, a backing layer and an enzyme material comprising salmon zonase.
- the patch comprises an enzyme material that selectively degrades only keratin and keratinaceous materials.
- the enzyme material is concentrated and protected from wash-out and degradation by the backing layer.
- the nature of the patch makes it easier to place the active enzyme material on the area that needs to be treated, which greatly increases its efficacy and cost-effectiveness.
- the enzyme material may be produced by fish of the genus Salmo, the enzyme material comprises one or more proteases capable of degrading human keratin or keratinaceous material. This solution offers a faster and, at the same time, safer way of treating hyperkeratosis.
- Proteases define a subclass of enzymes with the ability to cleave proteins into smaller parts.
- the enzyme material comprising zonase softens and thins hyperkeratotic formations on the skin, including calluses, corns, dry scaling/flaking skin and keratoses without harming the surrounding living tissue by selective lysing of hyperkeratotic tissue.
- the enzymes accomplish this by lysing only the 10-end peptide bonds of keratin protein, the dominant protein component of hyperkeratotic skin.
- Zonase is a fish-derived protease, displaying a high selectivity towards the degradation of human skin keratin, while leaving other proteins unaltered.
- Zonase is produced from natural sources, i.e. the Atlantic salmon, Salmo salar.
- Zonase is a non-self-degrading endoprotease which in nature serves as a hatching enzyme.
- Zonase is an endoprotease, but unlike most digestive enzymes which degrade water-soluble polypeptides to small oligopeptides or amino acids, zonase performs strategic cuts in the macromolecular structure of the types of keratin involved in hyperkeratosis.
- Zonase is a serine-protease, cleaving proteins at the carboxyl-terminal side of arginine and lysine residues.
- zonase is not a substrate for its own proteolysis, rendering the enzyme reasonably stable in aqueous or dry state, even in the absence of stabilizing agents. The enzyme is described in detail in US patent No. 6,592,866.
- Zonase can degrade keratin, thereby enhancing removal of dry and dead cells from the skin surface, but the enzyme does not attack living human cells, i.e. living tissue.
- the catalytic specificity of the zonase is unique as the enzyme shows no toxic effects on living human cells in culture.
- the enzyme material is water soluble and has an optimum pH for protease activity at about 7.2.
- the zonase enzyme for removal or thinning of keratinaceous tissue, the removal of dead skin cells is enhanced, without any harmful impact on living tissue. Keratin in human skin is only exposed in dead cells. The enzyme will selectively attack and degrade the dead cells, thereby thinning and over time totally removing the callous tissue to which it is applied.
- the invention also relates to a method for removing or thinning hyperkeratotic mammalian tissue comprising the steps of: obtaining an adhesive patch comprising a backing layer covered on one side by an adhesive layer, the patch comprising enzyme material comprising a salmon zonase, and applying said patch to said hyperkeratotic tissue.
- the patch may be especially suitable for application to corns and callous skin.
- the combination of active enzyme materials with adhesive patches can be technically complex.
- the enzyme material may be distributed in the adhesive of the patch or it may be located on the skin-facing surface of the patch.
- the enzyme material may be located in one or more separate portions constituting at least a part of the skin-facing surface of the patch.
- the enzyme containing portion may be partly embedded in the adhesive layer or the enzyme containing portion may in the form of a layer overlying a part of the second surface of the adhesive layer. Such layer may be in the form of a sheet or disc.
- the enzyme material may be incorporated in a pharmaceutically or cosmetically acceptable carrier.
- the carrier may be a water-based carrier or an oil-based carrier.
- the carrier may comprise petrolatum, poly(ethylene glycol) or cocoa butter or it may comprise a hydrogel.
- the carrier comprising the enzyme material may be in the form of one or more portions, for example in the form of discs being placed on the skin-contacting surface of the patch, or it may be located in indentations in the adhesive of the patch.
- the carrier may further comprise chemicals to regulate the pH and enzymatic activity of the enzyme or to help its action.
- a disc of the carrier material including the enzyme material may be an integrated part of the patch or the disc and the patch may be separate units being combined immediately before use.
- the patch comprises a backing layer located on the second surface of the patch and an adhesive layer on the first skin-facing surface of the patch, the adhesive layer facilitating an adhesive surface for attachment of the patch to the skin.
- a portion comprising the carrier with the enzyme material may be located in one or more indentations in the adhesive layer or on the skin-facing surface thereof.
- the carrier comprising the enzyme material and the adhesive layer may be separated by a barrier layer capable of preventing migration from one component to another.
- the adhesive may be any suitable skin compatible adhesive.
- the adhesive may contain hydrocolloids.
- the skin contacting surface of the enzyme containing portion may be designed to substantially correspond in size to corn, wart or callous.
- the patch may protect the corn or wart while applied, both by the relatively impermeable backing layer and it may also act as a cushion if the adhesive layer has a certain thickness. After use, the patch can easily be peeled off and discarded.
- the sizing of the enzyme carrying portion facilitates precise delivery of the enzyme only to the area to be treated. However, if the area of the enzyme containing material is larger than the corn thereby covering healthy living skin, the zonase will, contrary to other proteolytic enzymes, not attack the living skin cells and thereby cause damage.
- Enzymes may be expensive and having the enzyme located only on the spot where it is needed may reduce the cost price significantly.
- the patch renders it possible to treat a skin surface over a long period.
- the patch keeps the enzyme in place and protects it from being washed or worn out before the treatment is completed.
- the patch may be worn for several days allowing the enzyme to work
- the backing layer of the patch may be any layer, such as a polyurethane film, foam or non-woven or a combination of films or layers which, in combination with the adhesive, show the desired characteristics of the patch.
- the film may for example be produced from a polyolefinic material, polyvinyl acetate (pVAc), polyester, polyamid, polyurethane material or polyethylene or copolymers or blends thereof.
- the backing layer may be vapour and water impermeable, or it may be water impermeable but vapour permeable.
- an adhesive patch comprising a polyurethane backing layer and a hydrocolloid adhesive layer is employed.
- An enzyme containing disc is placed on the skin-facing adhesive surface of the patch.
- the disc weighs about 10 mg and contains 10 to 20 mil of the enzyme and is placed centrally on the adhesive surface of the patch.
- the disc may comprise an inert carrier material that is solid at room temperature but liquefies at the temperature of the human skin. This may be cocoa butter or poly(ethylene glycol) of an appropriate molecular weight.
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Biochemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Preparation (AREA)
- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/383,499 US20120123442A1 (en) | 2009-07-17 | 2010-07-14 | Adhesive patch with enzyme |
RU2012105529/15A RU2012105529A (en) | 2009-07-17 | 2010-07-14 | ADHESIVE PLASTER WITH ENZYME |
CN2010800315054A CN102471766A (en) | 2009-07-17 | 2010-07-14 | Adhesive patch with enzyme |
BR112012000905A BR112012000905A2 (en) | 2009-07-17 | 2010-07-14 | enzyme adhesive plaster |
EP10734658A EP2454366A2 (en) | 2009-07-17 | 2010-07-14 | Adhesive patch with zonase |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200970070 | 2009-07-17 | ||
DKPA200970070 | 2009-07-17 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2011006508A2 true WO2011006508A2 (en) | 2011-01-20 |
WO2011006508A3 WO2011006508A3 (en) | 2011-04-14 |
WO2011006508A8 WO2011006508A8 (en) | 2012-03-08 |
Family
ID=41119330
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK2010/050188 WO2011006508A2 (en) | 2009-07-17 | 2010-07-14 | Adhesive patch with zonase |
Country Status (6)
Country | Link |
---|---|
US (1) | US20120123442A1 (en) |
EP (1) | EP2454366A2 (en) |
CN (1) | CN102471766A (en) |
BR (1) | BR112012000905A2 (en) |
RU (1) | RU2012105529A (en) |
WO (1) | WO2011006508A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8992996B2 (en) * | 2012-01-23 | 2015-03-31 | Restorsea, Llc | Cosmetic |
US9248167B2 (en) | 2013-12-13 | 2016-02-02 | Restorsea, Llc | Exfoliative hair retention-promoting formulation |
US9433564B2 (en) | 2014-05-16 | 2016-09-06 | Restorsea, Llc | Biphasic cosmetic |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5213978A (en) | 1990-05-25 | 1993-05-25 | Scholl Plc | Protease from micrococcus sedentarius for degrading protein of human callus or corn tissue |
US5395613A (en) | 1990-05-25 | 1995-03-07 | Scholl Plc | Process for degradation of keratin, keratinaceous material, collagen and collagenaceous material with enzyme material, cell-free culture or cell culture from micrococcus sedentarius |
US6592866B2 (en) | 1997-12-11 | 2003-07-15 | Aqua Bio Technology As | Non-selfdegrading endoprotease |
US6858215B2 (en) | 2002-06-14 | 2005-02-22 | Carol J. Buck | Compositions and methods for softening, thinning and removing hyperkeratotic tissue |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5976556A (en) * | 1996-06-13 | 1999-11-02 | Active Organics, Inc. | Combination of acid protease enzymes and acidic buffers and uses thereof |
GR1002807B (en) * | 1996-06-20 | 1997-11-13 | Lavipharm A.E. | Device for topical treatment of acne and method of manufacture. |
NO314594B1 (en) * | 1997-12-11 | 2003-04-14 | Aqua Bio Technology As | Non-self-degrading endoprotease, method of preparation and applications thereof |
FR2783423B1 (en) * | 1998-09-23 | 2002-06-14 | Oreal | ASSET DELIVERY SYSTEM COMPRISING, ON A FILM STRUCTURE, A COMPOSITION BASED ON A SALICYLIC ACID DERIVATIVE, AND USES THEREOF |
US20030175328A1 (en) * | 2002-03-06 | 2003-09-18 | Adi Shefer | Patch for the controlled delivery of cosmetic, dermatological, and pharmaceutical active ingredients into the skin |
US7217853B2 (en) * | 2002-05-24 | 2007-05-15 | Corium International, Inc. | Composition for cushions, wound dressings and other skin-contacting products |
-
2010
- 2010-07-14 BR BR112012000905A patent/BR112012000905A2/en not_active Application Discontinuation
- 2010-07-14 WO PCT/DK2010/050188 patent/WO2011006508A2/en active Application Filing
- 2010-07-14 US US13/383,499 patent/US20120123442A1/en not_active Abandoned
- 2010-07-14 CN CN2010800315054A patent/CN102471766A/en active Pending
- 2010-07-14 EP EP10734658A patent/EP2454366A2/en not_active Withdrawn
- 2010-07-14 RU RU2012105529/15A patent/RU2012105529A/en unknown
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5213978A (en) | 1990-05-25 | 1993-05-25 | Scholl Plc | Protease from micrococcus sedentarius for degrading protein of human callus or corn tissue |
US5395613A (en) | 1990-05-25 | 1995-03-07 | Scholl Plc | Process for degradation of keratin, keratinaceous material, collagen and collagenaceous material with enzyme material, cell-free culture or cell culture from micrococcus sedentarius |
US6592866B2 (en) | 1997-12-11 | 2003-07-15 | Aqua Bio Technology As | Non-selfdegrading endoprotease |
US6858215B2 (en) | 2002-06-14 | 2005-02-22 | Carol J. Buck | Compositions and methods for softening, thinning and removing hyperkeratotic tissue |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8992996B2 (en) * | 2012-01-23 | 2015-03-31 | Restorsea, Llc | Cosmetic |
EP3100717B1 (en) | 2012-01-23 | 2021-10-06 | Restorsea, LLC | Cosmetic containing a fish spawn protein isolate |
US9248167B2 (en) | 2013-12-13 | 2016-02-02 | Restorsea, Llc | Exfoliative hair retention-promoting formulation |
US9433564B2 (en) | 2014-05-16 | 2016-09-06 | Restorsea, Llc | Biphasic cosmetic |
US9498430B1 (en) | 2014-05-16 | 2016-11-22 | Restorsea, Llc | Biphasic cosmetic |
Also Published As
Publication number | Publication date |
---|---|
EP2454366A2 (en) | 2012-05-23 |
RU2012105529A (en) | 2013-08-27 |
WO2011006508A3 (en) | 2011-04-14 |
WO2011006508A8 (en) | 2012-03-08 |
BR112012000905A2 (en) | 2016-03-01 |
US20120123442A1 (en) | 2012-05-17 |
CN102471766A (en) | 2012-05-23 |
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