WO2010116116A1

WO2010116116A1 - Process for preparing carboxylic acid amides useful in the treatment of muscular disorders

Info

Publication number: WO2010116116A1
Application number: PCT/GB2010/000386
Authority: WO
Inventors: David Selwood
Original assignee: Ucl Business Plc
Priority date: 2009-03-06
Filing date: 2010-03-03
Publication date: 2010-10-14
Also published as: HRP20130532T1; CN102414169B; CN102414169A; SI2403824T1; ES2424246T3; EP2403824B1; SMT201400042B; GB0903956D0; DK2403824T3; EP2403824A1; PT2403824E; CY1114331T1; US20120101301A1; PL2403824T3; US9024067B2

Abstract

The present invention relates to a process for preparing a compound of formula wherein: R² is cycloalkyl or alkyl, each of which may be optionally substituted; Y is -CONR³R⁴, -CN or CO₂R⁵; R³, R⁴ and R⁵ are each independently H or alkyl; n is 1 to 6; wherein said process comprising the steps of : (i) treating a compound of formula (IV), where R¹ is alkyl, with a compound of formula (V) and forming a compound of formula (IIIb); (ii) treating said compound of formula (IIIb) with a compound of formula (Il) to form a compound of formula (I).

Description

PROCESS FOR PREPARING CARBOXYLIC ACID AMIDES USEFUL IN THE TREATMENT OF

MUSCULAR DISORDERS

The present invention relates to a process for preparing compounds therapeutically useful in the treatment of muscular disorders, gastrointestinal disorders, or for controlling spasticity or tremors.

BACKGROUND TO THE INVENTION

WO2005/080316 (in the name of University College London) discloses compounds capable of modulating cannabinoid or cannabinoid-like receptors, including VSN-16, the structure of which is shown below.

VSN-16

Initial studies demonstrated that VSN-16 and related compounds exhibited a marked effect on spasticity in CREAE mice, providing strong evidence that a selective inhibition of spasticity was achieved without producing significant adverse CNS effects. Studies also demonstrated that the compounds inhibited gastrointestinal motility, as measured using a colonic propulsion test. More recent pharmacological studes have established that VSN-16 and related compounds appear to act on a putative novel cannabinoid receptor of the vasculature (P. M. Hoi, C. Visintin, M. Okuyama, S. M. Gardiner, T. Bennett, D. Baker, D. L. Selwood and C. R. Hiley; British Journal of Pharmacology, 2007, 1-14). VSN-16 is understood to act on the endothelium to release nitric oxide and activate K_Ca and TRPV₁. Its solubility is believed to play a significant role in bringing about peripheral cannabinoid-like effects without accompanying central or severe cardiovascular responses.

WO2005/080316 discloses the preparation of VSN-16 as shown in Scheme 1 below.

In brief, a palladium catalysed Songashira coupling reaction was used to insert a variety of alkyl side chains into 3-iodo methyl benzoate. The target compounds (S5) and related analogues were synthesised by a simple four-step route. First, the acid (S1) was reacted with DL alaninol in the presence of a diimide (EDCI) to give the amide (S2) in good yield. Palladium-catalysed coupling [Hoye, R. C. et a/, J. Org. Chem. 1999, 64, 2450-2453; Hopper, A. T. et a/, J. Med. Chem. 1998, 41, 420-427] of the amide with the alkyne acid in the presence of Cu¹I and pyrrolidine proceeded smoothly to give the alkyne (S3). The acid (S3) was quantitatively transformed into (S4) using ethylchloroformate and dimethylamine HCI. Lindlar catalysed reduction yielded the target alkene (S5). Alternatively, (S4) can be reduced with borohydride (polymer supported), (CH₃COO)₂Ni.4H₂O, MeOH, and H₂ at atmospheric pressure (P. M. Hoi, C. Visintin, M. Okuyama, S. M. Gardiner, T. Bennett, D. Baker, D. L. Selwood and C. R. Hiley; British Journal of Pharmacology, 2007, 1-14). The flexibility of this method allows the synthesis of a large number of different compounds using a range of alkynes for the Sonogashira coupling, or by starting with a different amine for the amide formation in the first step. However, the main drawback of this synthetic route is that the Lindlar catalytic reduction of intermediate (S4) yields a mixture of E- and Z- isomers of the resulting alkenyl compounds, requiring separation by reverse phase HPLC. This technique is both costly and time consuming, thereby rendering the method unsuitable for large scale synthesis. The present invention seeks to provide an alternative process for preparing VSN-16 and related compounds. More specifically - although not exclusively - the invention seeks to provide an improved process to those previously described in the art, and/or a process that is suitable for scale-up.

STATEMENT OF INVENTION

A first aspect of the invention relates to a process for preparing a compound of formula

I wherein:

R² is cycloalkyl or alkyl, each of which may be optionally substituted;

Y is -CONR³R⁴, -CN or CO₂R⁵;

R³, R⁴ and R⁵ are each independently H or alkyl; n is 1 to 6; said process comprising the steps of :

(i) treating a compound of formula IV, where R¹ is alkyl, with a compound of formula V to form a compound of formula IHb;

(ii) treating said compound of formula MIb with a compound of formula Il to form a compound of formula I;

A second aspect of the invention relates to a process for preparing VSN-16

VSN-16

said process comprising the steps of: treating a compound of formula IV.1 with a compound of formula V.1 to form a compound of formula IHb.1;

- treating said compound of formula HIb.1 with a compound of formula Mb.1 , where PG is a protecting group, to form a compound of formula Ib.1 ; and removing protecting group PG from said compound of formula Ib.1 to form

VSN-16

IV.1 V.I IIIb.1

VSN-16 Ib.l

DETAILED DESCRIPTION

The present invention relates to a process for preparing compounds of formula I, as defined herein.

As used herein, the term "alkyl" includes both saturated straight chain and branched alkyl groups which may be substituted (mono- or poly-) or unsubstituted. Preferably, the alkyl group is a C_L20 alkyl group, more preferably a

more preferably still a Ci_ ₁₀ alkyl group, more preferably still, a C-,.₆ alkyl group. Particularly preferred alkyl groups include, for example, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl and hexyl. Suitable substituents include, for example, alkyl, hydroxy, halo-, alkoxy-, nitro-, COOH, CO₂-alkyl, alkenyl, CN, NH₂, CF₃ or a cyclic group.

As used herein, the term "cycloalkyl" refers to a cyclic alkyl group which may be substituted (mono- or poly-) or unsubstituted. Preferably, the cycloalkyl group is a C_3-12 cycloalkyl group, more preferably a C_3-6 cycloalkyl group. Suitable substituents include, for example, alkyl, hydroxy, halo-, alkoxy-, nitro-, COOH, CO₂-alkyl, alkenyl, CN, NH₂, CF₃ or a cyclic group.

In one preferred embodiment, the invention relates to a process for preparing a compound of Ia

Ia wherein R², n and Y are as defined in claim 1.

In one preferred embodiment, n is 2, 3 or 4. Even more preferably, n is 3.

In one preferred embodiment, Y is -CONR³R⁴ or -CN. More preferably, Y is - CONR³R⁴. In one preferred embodiment, R³ and R⁴ are each independently H or methyl.

In one particularly preferred embodiment, Y is -CONMe₂ or CN. Even more preferably, Y is -CONMe₂.

In one preferred embodiment, R² is alkyl or cycloalkyl, each of which may be optionally substituted with OH or a halogen.

Where R² is cycloalkyl, preferably the cycloalkyl is a C₃-cycloalkyl, i.e. a cyclopropyl group.

In one preferred embodiment, R² is alkyl optionally substituted with OH or halogen. Preferably, the alkyl is branched. Even more preferably, R² is alkyl optionally substituted with OH.

In an even more preferred embodiment, R is

In one preferred embodiment, the invention relates to a process for preparing a compound selected from the following:

More preferably, the invention relates to a process for preparing a compound selected from the following:

In one highly preferred embodiment, the invention relates to a process for preparing VSN-16:

VSN-16

Step (i) - Wittig Reaction

Step (i) of the process involves a Wittig reaction (Maercker, A. GYg. React. 1965, 14, 270-490 (Review); W. Carruthers, Some Modern Methods of Organic Synthesis, Cambridge University Press, Cambridge, UK, 1971 , pp81-90; (ISBN 0-521-31117-9); R. W. Hoffmann (2001), Angewandte Chemie International Edition 40 (8): 1411-1416) between an aromatic aldehyde of formula IV and a triphenylphosphonium compound of formula V.

Preferably, the Wittig reaction is carried out using a compound of formula V containing an amide group or a free acid group.

Thus, in one preferred embodiment, the compound of formula V used in step (i) is an amide compound of formula Va

Va wherein R³ and R⁴ are both alkyl, more preferably methyl.

In one preferred embodiment of the process, the compound of formula V used in step (i) is dissolved in dichloromethane. Preferably, the dichloromethane is anhydrous.

In one preferred embodiment, step (i) comprises treating the compound of formula V with potassium hexamethyldisilazide in THF or toluene prior to addition of the compound of formula IV.

Preferably, the potassium hexamethyldisilazide is added at a temperature of less than about 5 ⁰C. More preferably, the temperature is about 0 ⁰C.

In one preferred embodiment, the compound of formula IV is dissolved in THF.

Preferably, the compound of formula IV is added to the reaction mixture at a temperature of less than about 5 ⁰C, more preferably less than about 4 ⁰C, even more preferably, less than about 3 ⁰C.

The compound of formula Va may itself be prepared from a compound of formula Vb. Thus, in one preferred embodiment, the process of the invention further comprises the step of preparing a compound of formula Va from a compound of formula Vb.

Vb Va

Preferably, the process comprises treating said compound of formula Vb with (a) ethyl chloroformate and triethylamine to form a mixed anhydride; and (b) reacting the mixed anhydride with an amine salt NHR³R⁴.HCI. More preferably, the amine salt NHR³R⁴. HCI is dimethylamine.HCI.

Preferably, step (a) is carried out in THF or anhydrous dichloromethane.

Preferably, the amine salt NHR³R⁴.HCI used in step (b) (e.g. dimethylamine.HCI) is recrystallised from methanol/diethyl ether prior to use. Preferably, the compound of formula Va is purified by trituration with diethyl ether.

As mentioned above, the Wittig reaction can also be carried out using a compound of formula V containing a free acid group. Thus, in an alternative preferred embodiment of the invention, the compound of formula V used in step (i) is of formula Vb

Vb

Preferably, for this embodiment, step (i) comprises adding sodium hydride in a mixture of anhydrous dichloromethane and anhydrous THF to a mixture of said compound of formula Vb in anhydrous dichloromethane.

In one preferred embodiment, step (i) comprises hydrolysing the crude product formed from the reaction of Vl with V. Preferably, the crude product is hydrolysed with aqueous sodium hydroxide in methanol.

Separation of isomers HIa and 1Mb

The Wittig reaction of step (i) yields a mixture of isomers Ilia and 1Mb, corresponding to the E- and Z-isomers respectively.

ma nib Isomer Ilia is preferably removed and isomer IHb taken through to the final product.

Preferably, the process of the invention comprises the step of separating isomer 1Mb from the mixture of isomers Ilia and 1Mb prior to the commencement of step (ii)

In one particularly preferred embodiment of the invention, isomers HIa and IUb are separated by forming their respective salts. Advantageously, the salts of isomers IHa and 1Mb can be readily separated in view of their differing solubilities.

Thus, one highly preferred embodiment of the invention comprises the steps of:

(a) forming a salt of isomers Ilia and IUb;

(b) separating the salt of isomer HIb from the salt of isomer Ilia; and

(c) treating the salt of isomer lllb obtained in step (b) to form isomer lllb.

Preferably, the salt forms of isomers Ilia and IHb can be separated by crystallisation, i.e. step (b) comprises separating the salt of isomer lllb from the mixture by crystallisation. Advantageously, this avoids the need for costly and time consuming purification using reverse phase HPLC, as required by processes for preparing VSN- 16 and analogues thereof known in the art to date. Moreover, the ability to separate the E- and Z-isomers Ilia and lllb by crystallisation renders the process suitable for scale-up and contributes to an improved overall yield.

Any suitable salt can be used, providing that the salt form of isomers HIa and HIb can be readily separated by routine techniques. Suitable salts will be familiar to the skilled person.

In one preferred embodiment, the process involves forming the 4- dimethylaminopyridine (DMAP) salt. Thus, step (a) comprises treating the mixture of isomers Ilia and lllb with 4-dimethylaminopyridine (DMAP) to form the corresponding DMAP salts.

Preferably, the DMAP is dissolved in ethyl acetate.

In one particularly preferred embodiment, step (b) comprises crystallising the salt form of isomer IiIb from a solvent mixture of diethyl ether and ethyl acetate. More preferably, the solvent mixture is a mixture of 1 :100 to 100:1 or 1 :50 to 50:1 , more preferably 1 :20 to 20:1 , even more preferably 1 :10 to 10:1 diethyl ether : ethyl acetate. Even more preferably, the solvent mixture is 9:1 diethyl ether : ethyl acetate.

In one preferred embodiment, step (c) comprises treating the salt of isomer IMb with an acid to form isomer IHb (in the free acid form). Preferably, the acid is HCI.

Step (ii)

Step (ii) of the process comprises reacting the compound of formula MIb with a compound of formula Il to form a compound of formula I.

In one preferred embodiment, step (ii) comprises reacting said compound of formula HIb with a compound of formula Il in the presence of a coupling agent. Suitable coupling agents will be familiar to the skilled person.

In one particularly preferred embodiment, the coupling agent is 1 ,1'- carbonyldiimidazole (CDI).

Preferably, for this embodiment, step (ii) comprises dissolving said compound of formula IHb and CDI in anhydrous DMF and adding thereto said compound of formula Il in anhydrous DMF.

In another particularly preferred embodiment, the coupling agent is 1-(3- dimethylaminopropyl)-ethylcarbodiimide hydrochloride (EDCI).

Preferably, for this embodiment, step (ii) comprises dissolving said compound of formula IHb and ECDI in anhydrous dichloromethane and adding thereto N-ethyl diisopropylamine and said compound of formula II.

In one highly preferred embodiment of the invention, step (ii) comprises treating said compound of formula IHb with a compound of formula lib to form a compound of formula Ib

where PG is a hydroxyl protecting group. Suitable hydroxyl protecting groups will be familiar to the skilled person in the art (see for example, "Protective Groups in Organic Chemistry", Theodore W. Greene; John Wiley & Sons, Inc., New York, 1991 , ISBN 0- 471-62301-6).

Preferably, the hydroxyl protecting group is a silyl protecting group.

More preferably, the hydroxyl protecting group is selected from triisopropyl and trimethylsilyl.

Thus, in one preferred embodiment, the process comprises treating 2-amino-1- propanol with chorotrimethylsilane and imidazole in dichloromethane to form a trimethylsilyl-protected compound of formula Mb,

Preferably, the trimethylsilyl-protected compound of formula lib is used directly in step (ii) in solution form without further purification.

In another preferred embodiment, the process comprises treating 2-amino-1 -propanol in anhydrous dichloromethane with 2,6-lutidine and triisopropylsilyl trifluromethane sulfonate to form a triisopropylsilyl-protected compound of formula Mb,

Preferably, the triisopropylsilyl-protected compound of formula lib is used directly in step (ii) without further purification.

In one highly preferred embodiment of the invention, the compound of formula Hb is of the formula

.O-PG

H₂N

Preferably, the process of the invention further comprises the step of removing the protecting group PG from said compound of formula Ib to form a compound of formula Ia

Ib Ia

Suitable deprotecting agents and conditions will be familiar to the skilled person (see for example, "Protective Groups in Organic Chemistry", Theodore W. Greene; John Wiley & Sons, Inc., New York, 1991 , ISBN 0-471-62301-6).

In one preferred embodiment, the protecting group PG is removed by treating with TBAF. Preferably, the solvent is THF.

Synthesis of VSN-16

A second aspect of the invention relates to a process for preparing VSN-16

said process comprising the steps of:

- treating a compound of formula IV.1 with a compound of formula V.1 to form a compound of formula IHb.1 ;

- treating said compound of formula lllb.1 with a compound of formula I Ib- 1 , where PG is a protecting group, to form a compound of formula Ib.1 ; and removing protecting group PG from said compound of formula Ib.1 to form VSN-16

IV.1 V.I IIIb.1

VSN-16 Ib.l

Preferably, protecting group PG, and the reaction conditions, solvent, temperature and the like, are as described above for the first aspect of the invention. Step (i) of the process yields a mixture of isomers HIa.1 and lllb.1 , which may be separated by the methodology described for the first aspect of the invention, e.g. by converting to salt form and separating by crystallisation.

The present invention is further described by non-limiting example. EXAMPLES

One preferred embodiment of the claimed process is set forth in Scheme 2, further details of which are described in the following examples.

(b)

Scheme 2: (a) (i) anhydrous CH₂CI₂, potassium hexamethyl disilazide, THF under N₂ atmosphere, < 10 ⁰C; (ii) NaOH, MeOH; (b) (i) DMAP (EtOAc, Et₂O); (ii) separation of isomers; (iii) HCI; (c) (i) EDCI, CH₂CI₂, N-ethyl diisopropylamine or (ii) CDI, DMF N-ethyl diisopropylamine; (d) TBAF/THF.

N,N-dimethylamino 4-(carboxybutyl) triphenylphosphonium bromide 4-(carboxybuty!)triphenylphosphonium bromide (14Og, 0.315 mol, 1 equiv) was charged in a reactor and dichloromethane (650ml, 4.5 vols) was added. Triethylamine (dried on molecular sieves; 95ml, 2.1 equiv) was charged and the reaction mixture was cooled down to-10°C. Ethyl chloroformate (40ml, 1.05 equiv) was added dropwise and the mixture was stirred for another 15 min at -1O⁰C.

A solution containing dimethylamine hydrochloride (freshly crystallised from methanol/ether; 78g, 3 equiv) and triethylamine (200ml, 4.5 equiv) in dichloromethane (1000ml, 7 vols) was prepared. This solution was stirred for 40 min at room temperature and added dropwise to the reaction mixture at -1O⁰C. The temperature was kept between -10 and -15⁰C during all the addition. The reaction was left to warm up to room temperature. The reaction was stirred at room temperature overnight. The mixture was treated with 2I of saturated NaHCO₃ solution. The aqueous phase was extracted with dichloromethane (1x 2I and

2x11). Organics were combined and dried over MgSO₄ and filtered. The volatiles were removed under vacuum. The residue was triturated with 350 ml of diethyl ether. The solid was filtered and triturated with hot diethyl ether for 5hours. The suspension was cooled down and the solid filtered. The solid was dried under vacuum to give 130.9g of a white solid (90% yield).

¹H NMR (CDCI₃) 7.65-8.0 (m, 15H); 3.7 (m, 2H); 3.0 (s, 3H); 2.8 (s, 3H), 2.5 (t, J = 7Hz, 2H); 1.9 (m, 2H), 1.7 (m, 2H).

3-[(1Z)-6-(dimethylamino)-6-oxohex-1-en-1-yll benzoic acid

N,N-dimethylamino 4-carboxybutyltriphenylphosphonium (61.9g, 0.13 mol, 3 equivalents) were dissolved in dry dichloromethane (150ml, 2.4vols) under nitrogen. The solution was cooled down to O⁰C and potassium hexamethyldisilazide (0.9M in THF; 45ml, 5 equiv) was added dropwise at O⁰C. The reaction mixture was stirred at O⁰C for another 45 min. A solution of methyl 3-formylbenzoate (7.16g, 1 equiv) in dry THF (36ml, 5vols) was added keeping the temperature <4°C. The mixture was allowed to warm up to room temperature and was stirred for 18hrs. The reaction was quenched with 2M HCI (400ml) and extracted with dichloromethane (2 x 400ml and 2 x 200ml). Organics were combined, dried over MgSO₄, filtered and evaporated to dryness. The residue was dissolved in a mixture of sodium hydroxide 1 M/methanol 4:1 (440ml) and stirred for 18 hrs. Water (100ml) was added to the mixture and methanol was evaporated under vacuum. Aqueous was extracted with ethyl acetate (400ml). The pH was adjusted to pH 1 and the mixture was extracted with dichloromethane (2 x 400ml and 2 x 200ml). Organics were dried over MgSO₄, filtered and evaporated to dryness. M = 22.Og. The crude was purified by flash chromatography using dichloromethane to dichloromethane/MeOH = 95/54 as eluent. M = 10.6g 93% yield.

Isomer separation

Acid (10.93g, 0.042mol) was dissolved in ethyl acetate (20ml) and 4- dimethylaminopyridine (6.13g, 1.2 equiv) was dissolved in warm ethyl acetate (20ml). The DMAP solution was added to the free acid solution. The mixture was stirred at reflux temperature for 10 min. Then, the solution was allowed to cool down to room temperature slowly. A brown salt was formed, which was removed by filtration.

A mixture of diethyl ether/ethyl acetate: 9:1 (40ml) was added and the solution was heated to reflux. The mixture was stirred and allowed to cool down overnight. A pale yellow solid was filtered and dried in-vacυo. This solid was treated with HCI (1M) and extracted with dichloromethane (3 x 50ml). Organics were dried over MgSO₄, filtered and evaporated to dryness to give a brown oil which solidified upon standing. M = 3.88g (35.5% yield).

¹H NMR (CDCI₃) 9.7 (bs, 1 H); 8.0 (m, 2H); 7.5 (m, 2H); 6.5 (d, J = 1 1 Hz, 1 H); 5.75 (m, 1H); 3.0 (s, 6H); 2.4 (m, 4H); 1.9 (m, 2H)

(R)-2-amino-1-triisopropylsilyloxypropanol

(R)-2-amino-1-propanol (1.0g, 0.0133 mol) was dissolved in dry dichloromethane (5ml, 5 vols) and 2,6-lutidine (1.75ml, 0.0146 mol, 1.1 equiv) was added then trisiopropylsilyl trifluoromethane sulfonate (4ml, 0.0146 mol, 1.1 equiv) at room temperature. The temperature was controlled with a water bath. The reaction was stirred overnight at room temperature. TLC showed formation of a second spot and no trace of starting material. The mixture was washed with 15% aqueous acetic acid (3ml). Organics were separated, dried over MgSO₄, filtered and evaporated to dryness to provide a brown thick oil. Petrol ether was added and a white solid formed. The solid was filtered off and the filtrate was evaporated to provide the product with a quantitative yield. The product was used without further purification.

¹H NMR (CDCI₃) 6.5 (bs, 2H); 3.8 (m, 1 H); 3.6 (m, 1 H); 3.4 (m, 1 H); 1.4 (d, J = 7Hz, 3H)m, 1.0 (m, 21 H).

3-[(1Z)-6-(dimethylamino)-6-oxohex-1-en-1-yll-N-(2~triisopropylsilyloxy-1 -methyl- ethyflbenzamide

The 3-[(1Z)-6-(dimethylamino)-6-oxohex-1-en-1-yl] benzoic acid previously prepared (2.19g, 0.0083 mol, 1 equiv) and 1 ,1-carbonyldiimidazole (1.87g, 0.0115 mol, 1.4 equiv) were dissolved in dry dimethylformamide (15ml, 7 vols) at O⁰C. (R)-2-amino-1- triisopropylsilyloxypropanol was dissolved in a small portion of dry DMF and added. The reaction mixture was stirred for 18h at 5O⁰C. DMF was removed under vacuum and the residue was co-evaporated with toluene to remove traces of DMF. The crude was dissolved in ethyl acetate (100ml) and washed with 2M HCI (50ml), saturated sodium bicarbonate (60ml), water (2 x 50ml) and brine (50ml). Organics were dried over MgSO₄, filtered and solvent was evaporated under vacuum. The residue was purified by chromatography on silica using ethyl acetate/petrol :2:8 to 9:1 as eluent. This provided the expected product as a white solid with 73.6% yield.

¹H NMR (CDCI₃) 7.6 (s, 1 H); 7.5 (m, 1 H); 7.3 (d, m, 2H); 6.6 (d, J = , 1 H; 6.4 (d, J = 1 H); 5.65 (m, 1 H); 4.2 (m, 1 H); 3.7 (m. 2H); 2.9 s, 3H); 2.85 (s, 3H); 2.35 (m, 2H); 2.25 (t, J = 14Hz, 2H); 1.75 (m, 2H); 1.25 (d, J = 7Hz, 3H), 1.0 (m, 21 H)

(R)-3-(5-Dimethylcarbamoyl-pent- 1-enyl)-N-(2-hydroxy- 1 -methyl-ethyl) benzamide (VSN 16R) 3-[(1Z)-6-(dimethylamino)-6-oxohex-1-en-1-yl]-Λ/-(2-triisopropylsilylether-1 -methyl- ethyl) benzamide (2.87g, 0.0061 mol) was dissolved in THF (18ml, 6 vols) and tetra n- butylammonium fluoride 1 M in THF (18ml, 3 equiv, 0.018 mol) was added at room temperature. The reaction mixture was stirred at room temperature for 18h. The solvent was evaporated and ethyl acetate (50ml) was added. The mixture was washed with HCL2M (50ml) and brine (50ml). Organics were dried over MgSO4, filtered and evaporated to dryness. The residue was purified by chromatography on silica using dichloromethane then dichloromethane/MeOH: 95/5 to provide only 438mg of product (27.9%) as a thick yellow oil. TLC showed that some product remained in the aqueous layer. The aqueous was further extracted with ethyl acetate until no product was left in the aqueous. The crude was purified as previously to provide a thick yellow oil. The product sticks to ethyl acetate and was dissolved in dichloromethane and evaporated to dryness. M = 1.3g (70.3% yield).

¹H NMR (CDCI₃) 7.7 (m, 2H); 7.35 (m, 1 H); 7.2 (m, 1 H); 6.4 (d, J = 12Hz, 1 H); 5.65 (m, 1 H), 4.2 (m, 1 H); 3.75 (dd, J = 3Hz, J = 8Hz, 1 H); 3.55 (dd, J = 5Hz, J = 11 Hz, 1 H); 2.9 (s, 3H); 2.85 (s, 3H); 2.3 (t, J = 7Hz, 2H); 2.25 (m, 2H); 1.8 (m, 2H); 1.25 (d, J = 7Hz, 3H)

Various modifications and variations of the described methods of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, various modifications of the described modes for carrying out the invention which are obvious to those skilled in chemistry or related fields are intended to be within the scope of the following claims.

Claims

1. A process for preparing a compound of formula I₁

I wherein:

R² is cycloalkyl or alkyl, each of which may be optionally substituted;

Y is -CONR³R⁴, -CN or CO₂R⁵;

(i) treating a compound of formula IV, where R¹ is alkyl, with a compound of formula V to form a compound of formula IMb; (ii) treating said compound of formula INb with a compound of formula Il to form a compound of formula I;

2. A process according to claim 1 wherein said compound of formula I is of formula Ia

Ia wherein R², n and Y are as defined in claim 1.

3. A process according to claim 1 or claim 2 wherein n is 2, 3 or 4.

4. A process according to any preceding claim wherein Y is -CONR R or -CN.

5. A process according to any preceding claim wherein Y is -CONMe₂.

6. A process according to any preceding claim wherein R² is alkyl optionally substituted with OH.

7. A process according to any preceding claim wherein R² is

8. A process according to any preceding claim wherein said compound of formula I is selected from the following:

9. A process according to any preceding claim wherein said compound of formula l is VSN-16

10. A process according to any preceding claim wherein said compound of formula V is of formula Va

Ph₃P (CH₂)_n ^/^NR³R⁴

Va wherein R³ and R⁴ are both alkyl.

11. A process according to claim 10 wherein R³ and R⁴ are both methyl.

12. A process according to claim 10 or 11 which further comprises the step of preparing a compound of formula Va from a compound of formula Vb.

Vb Va

13. A process according to claim 12 which comprises treating said compound of formula Vb with (a) ethyl chloroformate and triethylamine to form a mixed anhydride; and (b) reacting said mixed anhydride with an amine salt NHR³R⁴. HCI.

14. A process according to claim 13 wherein step (a) is carried out in THF or anhydrous dichloromethane.

15. A process according to claim 13 or claim 14 wherein the amine salt NHR³R⁴. HCI used in step (b) is recrystallised from methanol/diethyl ether prior to use.

16. A process according to any one of claims 10 to 15 wherein said compound of formula Va is purified by trituration with diethyl ether.

17. A process according to any preceding claim wherein said compound of formula V is dissolved in anhydrous dichloromethane.

18. A process according to any preceding claim wherein step (i) comprises treating the compound of formula V with potassium hexamethyldisilazide in THF or toluene prior to addition of the compound of formula IV.

19. A process according to claim 18 wherein the potassium hexamethyldisilazide is added at a temperature of less than about 5 ⁰C.

20. A process according to any one of claims 1 to 9 wherein said compound of formula V is of formula Vb

Vb

21. A process according to claim 20 wherein step (i) comprises adding sodium hydride in a mixture of anhydrous dichloromethane and anhydrous THF to a mixture of said compound of formula Vb in anhydrous dichloromethane.

22. A process according to any preceding claim wherein said compound of formula IV is dissolved in THF.

23. A process according to any preceding claim wherein the compound of formula IV is added to the reaction mixture at a temperature of less than about 5 ⁰C.

24. A process according to any preceding claim wherein step (i) comprises hydrolysing the crude product of the reaction of said compound of formula IV and said compound of formula V.

25. A process according to any preceding claim which comprises the step of isolating isomer NIb from a mixture of isomers MIa and HIb prior to step (ii).

IHa nib

26. A process according to claim 25 which comprises the steps of:

(a) forming a salt of isomers IMa and 1Mb;

(b) separating the salt of isomer NIb from the salt of isomer Ilia; and

(c) treating the salt of isomer IHb obtained in step (b) to form isomer IHb.

27. A process according to claim 26 wherein step (a) comprises treating the mixture of isomers Ilia and 1Mb with 4-dimethylaminopyridine (DMAP) to form the corresponding DMAP salts.

28. A process according to claim 27 wherein the DMAP is dissolved in ethyl acetate.

29. A process according to any one of claims 26 to 28 wherein step (b) comprises separating the salt form of isomer 1Mb by crystallisation.

30. A process according to claim 29 wherein step (b) comprises crystallising the salt form of isomer 1Mb from a solvent mixture of diethyl ether and ethyl acetate.

31. A process according to any one of claims 26 to 30 wherein step (c) comprises treating the salt form of isomer IMb with an acid to form isomer MIb.

32. A process according to claim 31 wherein the acid is HCI.

33. A process according to any preceding claim wherein step (ii) comprises reacting said compound of formula IMb with a compound of formula Il in the presence of a coupling agent.

34. A process according to claim 33 wherein the coupling agent is 1 ,1'- carbonyldiimidazole (CDI).

35. A process according to claim 34 wherein step (ii) comprises dissolving said compound of formula IMb and CDI in anhydrous DMF and adding thereto said compound of formula Il in anhydrous DMF.

36. A process according to claim 33 wherein the coupling agent is 1-(3- dimethylaminopropyl)-ethylcarbodiimide hydrochloride (EDCI).

37. A process according to claim 36 wherein step (ii) comprises dissolving said compound of formula IHb and ECDI in anhydrous dichloromethane and adding thereto N-ethyl diisopropylamine and said compound of formula II.

38. A process according to any preceding claim wherein step (ii) comprises treating said compound of formula MIb with a compound of formula lib to form a compound of formula Ib

UIb Ib wherein PG is a hydroxyl protecting group.

39. A process according to claim 38 wherein the hydroxyl protecting group is a silyl protecting group.

40. A process according to claim 38 or claim 39 wherein the hydroxyl protecting group is selected from triisopropyl and trimethylsilyl.

41. A process according to claim 40 which comprises treating 2-amino-1-propanol with chorotrimethylsilane and imidazole in dichloromethane to form a trimethylsilyl- protected compound of formula Hb.

42. A process according to claim 41 wherein the trimethylsilyl-protected compound of formula Mb is used directly in step (ii) in solution form without further purification.

43. A process according to claim 40 which comprises treating 2-amino-1-propanol in anhydrous dichloromethane with 2,6-lutidine and triisopropylsilyl trifluromethane sulfonate to form a triisopropylsilyl-protected compound of formula lib.

44. A process according to claim 43 wherein the triisopropylsilyl-protected compound of formula Hb is used directly in step (ii) without further purification.

45. A process according to any one of claims 38 to 44 wherein said compound of formula Nb is of the formula

)-PG

H₂N'

46. A process according to any one of claims 38 to 45 which further comprises the step of removing the protecting group PG from said compound of formula Ib to form a compound of formula Ia

47. A process according to claim 46 which comprises treating said compound of formula Ib with TBAF in THF.

48. A process for preparing VSN-16

said process comprising the steps of: treating a compound of formula IV.1 with a compound of formula V.1 to form a compound of formula IHb.1 ; treating said compound of formula MIb.1 with a compound of formula I Ib.1 , where PG is a protecting group, to form a compound of formula Ib.1 ; and removing protecting group PG from said compound of formula Ib.1 to form

VSN-16

IV.1 V.I IHb.1

VSN-16 Ib.l

49. A process substantially as described herein and with reference to the accompanying examples.