WO2010084160A1 - Phenylcyclopropylamine derivatives and their medical use - Google Patents

Phenylcyclopropylamine derivatives and their medical use Download PDF

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Publication number
WO2010084160A1
WO2010084160A1 PCT/EP2010/050697 EP2010050697W WO2010084160A1 WO 2010084160 A1 WO2010084160 A1 WO 2010084160A1 EP 2010050697 W EP2010050697 W EP 2010050697W WO 2010084160 A1 WO2010084160 A1 WO 2010084160A1
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WIPO (PCT)
Prior art keywords
trans
alkyl
aryl
phenylcyclopropanamine
thiocarbamyl
Prior art date
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PCT/EP2010/050697
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French (fr)
Inventor
Nathalie Guibourt
Alberto ORTEGA MUÑOZ
Julio Castro-Palomino Laria
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Oryzon Genomics S.A.
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Publication date
Application filed by Oryzon Genomics S.A. filed Critical Oryzon Genomics S.A.
Priority to EP10704336.6A priority Critical patent/EP2389362B1/en
Priority to US13/138,143 priority patent/US8993808B2/en
Publication of WO2010084160A1 publication Critical patent/WO2010084160A1/en
Priority to US14/627,333 priority patent/US20160045456A1/en

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    • A61K31/13—Amines
    • A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
    • A61K31/137—Arylalkylamines, e.g. amphetamine, epinephrine, salbutamol, ephedrine or methadone
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    • A61K31/14—Quaternary ammonium compounds, e.g. edrophonium, choline
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    • A61K31/343—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide condensed with a carbocyclic ring, e.g. coumaran, bufuralol, befunolol, clobenfurol, amiodarone
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    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
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Definitions

  • the present invention relates to phenylcyclopropylamine derivatives.
  • pharmaceutical compositions comprising phenylcyclopropylamine derivatives are provided.
  • the compounds of this invention can, inter alia, be used for the treatment and the prevention of cancer as well as neurodegenerative diseases or disorders.
  • Cancer is prevalent: there were about 3.2 million cancer cases diagnosed (53% men, 47% women) and 1.7 million deaths from cancer (56% men, 44% women) in Europe (Ferlay et al. (2007) Ann. Oncol. 18(3):581-92). In the United States, the probability of developing invasive cancer is 38% for females and 46% for males that live to be 70 years older and older. In the US about 1.4 million new cases of cancer are expected for 2006. Although the five year survival rate for cancer is now 65%, up from about 50% in the mid-nineteen seventies, cancer is deadly. It is estimated that 565,000 people in the United States will die from cancer in 2006 (American Cancer Society, Surveillance Research, 2006). Despite tremendous advances in cancer treatment and diagnosis, cancer remains a major public health concern. Accordingly, there is a need for new therapeutics with activity in cancer.
  • Alzheimer's disease is the largest neurodegenerative disease; disease modifying drugs have long been sought, but to-date, none have been identified.
  • Other neurodegenerative conditions include Parkinson's disease, Huntington ' s disease, Lewy Body dementia, and which are all characterized by disease progression which robs the patients of their ability to perform normal daily activities, eventually leading to death.
  • One similar characteristic amongst many cancers and neurodegenerative diseases is aberrant gene expression.
  • a number of compounds have been shown to alter gene expression, including histone deacetylase inhibitors which alter the histone acetylation profile of chromatin.
  • Histone deacetylase inhibitors have been shown to alter gene expression. Another modification that is involved in regulating gene expression is lysine methylation. Methylation of histone lysines has recently been shown to be important in regulating gene expression. A group of enzymes known as lysine demethylases are involved in this histone modification.
  • lysine demethylases One particular human histone lysine demethylase enzyme called Lysine Specific Demethylase-1 (LSDl) was recently discovered (Shi et al. (2004) Cell 1 19:941). LSDl is also involved in regulating the methylation of some non-histone lysines.
  • LSDl has a fair degree of structural similarity, and amino acid identity/homology to monoamine oxidases, all of which (i.e., MAO-A, MAO-B and LSDl) are flavin dependent oxidases.
  • MAO-A, MAO-B and LSDl are flavin dependent oxidases.
  • Recent experiments with LSDl have shown that it is involved in diverse process such as carcinogenesis (Kahl et al. (2006) Cancer Res. 66: 1341-1 1347) and vascular inflammation (Reddy et al. (2008) Circ. Res. 103 :615). It was found that a commercially available antidepressant, Parnate®, which targets monoamine oxidase (MAO), also inhibits LSDl at clinically relevant concentrations (Lee et al. (2006) Chem. Biol.
  • Monoamine oxidase inhibitors are useful for treating a number of conditions including depression and neurodegenerative conditions like Parkinson's disease.
  • Parnate is part of a class of compounds known as phenylcyclopropylamines which are related to another group of clinical useful MAO inhibitors called propargylamines, exemplified by Pargyline which also inhibits LSDl . Additionally, derivatives of Parnate also can inhibit LSDl (Gooden et al. (2008) Bioorg. Med. Chem. Let. 18:3047-3051). Another class of compounds was recently disclosed to inhibit LSDl activity: polyamines (Huang et al. (2007) PNAS 104:8023-8028).
  • LSDl expression is correlated with prostate cancer aggressiveness.
  • Metzger et al. ((2005) Nature 15;437(7057):436-9) reported that LSDl modulation by siRNA and pargyline regulates androgen receptor (AR) and may have therapeutic potential in cancers where AR plays a role, like prostate, testis, and brain cancers.
  • AR androgen receptor
  • the phenylcyclopropylamines have been the subject of many studies designed to elucidate an SAR for MAO inhibition.
  • Kaiser et al. ((1962) J. Med. Chem. 5: 1243- 1265)
  • Zirkle et al. ((1962) J. Med. Chem. 1265-1284) have disclosed the synthesis and activity of a number of phenylcyclopropylamine related compounds.
  • Zirkle et al. ((1962) J. Med. Chem.
  • Phenylcyclopropylamine derivatives are also disclosed in US-A-3, 106,578, US-A-6,21 1 ,244, US-A-2003/236225, WO 03/093297, WO 2007/025144, and in Westland RD, et al. J Med Chem. 1968. 11 (4): 824-829.
  • the present invention relates to phenylcyclopropylamine derivatives.
  • pharmaceutical compositions comprising phenylcyclopropylamine derivatives are provided.
  • the compounds of this invention can, inter alia, be used for the treatment and the prevention of diseases.
  • the present invention provides compounds of Formula I, pharmaceutical compositions comprising a compound of Formula I and a pharmaceutically acceptable carrier, and their use for treating and/or preventing diseases.
  • One use of the compounds of Formula I is for treating cancer.
  • Another use for the compounds of Formula I is to inhibit LSDl .
  • Another use of the compounds of Formula I is as dual inhibitors of MAO-B and LSDl .
  • Compounds of Formula I can have monoamine oxidase inhibition activity and therefore can be used to treat and/or prevent disease like depression and Parkinson's disease as well as other neurodegenerative conditions.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a compound of Formula I and a pharmaceutically acceptable carrier:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkyny ⁇ , arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbony
  • R6 is chosen from -H and alkyl
  • R7 is chosen from -H, alkyl, and cycloalkyl
  • R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyana
  • R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sul
  • R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, isocyanato, is
  • each L is independently chosen from -(CHi) n -(CHi) n -, - (CHi) n NH(CHi) n -, -(CHi) n O(CHi) n -, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CHa) n -(CH 2 )H- and -(CHi) n O(CHa) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl
  • R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, isocyanato, is
  • R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sul
  • each L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, - (CH 2 )nNH(CH 2 ) n -, -(CH 2 )n0(CH 2 )n-, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH2) n -(CH 2 ) n - and -(CHi) n O(CHi) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I which is a selective inhibitor of LSDl .
  • LSD l selective inhibitors have IC50 values for LSDl which are at least 2- fold lower than the IC50 value for MAO-A and/or MAO-B.
  • the invention also provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I which is a dual inhibitor selective for LSDl and MAO-B.
  • Dual LSD1/MAO-B selective inhibitors have IC50 values for LSDl and MAO-B which are at least 2-fold lower than the IC50 value for MAO-A.
  • the invention provides a compound of Formula I for treating and/or preventing cancer:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl,
  • R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyana
  • the cancer is prostate cancer. In another specific aspect of this embodiment, the cancer is brain cancer. In yet another specific aspect of this embodiment, the cancer is breast cancer. In yet another specific aspect of this embodiment, the cancer is lung cancer. In yet another specific aspect of this embodiment, the cancer is testicular cancer. In yet another specific aspect of this embodiment, the cancer is colorectal cancer. In yet another specific aspect of this embodiment, the cancer is blood cancer (e.g., leukemia). In yet another specific aspect of this embodiment, the cancer is skin cancer.
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, isothiocyana
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro,
  • the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, isothiocyanato,
  • each L is independently chosen from -(CH 2 )n-(CH 2 )n-, -(CH 2 ) n NH(CH 2 )n-, -(CH 2 ) n O(CH 2 )n-, and -(CHi) n S(CH 2 )Ii-, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and - (CHi) n O(CHi) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sul
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, isothiocyanato,
  • each L is independently chosen from - (CH 2 ) n -(CH 2 ) n -, -(CH 2 )nNH(CH 2 ) n -, -(CH 2 ) n O(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbony
  • R6 is chosen from -H and alkyl
  • R7 is chosen from -H, alkyl, and cycloalkyl
  • R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyana
  • the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder
  • R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothio
  • the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder
  • R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, iso
  • the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, is
  • each L is independently chosen from -(CHa) n -(CHa) n -, - (CH 2 )nNH(CH 2 ) n -, -(CH 2 )J 1 O(CH 2 ),,-, and -(CH 2 )nS(CH 2 )n-, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CHa) n -(CHi) n - and -(CHa) n O(CHa) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In a more specific definition, L is a covalent bond.
  • the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder where R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyan
  • the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder
  • R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, is
  • the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder
  • R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothi
  • each L is independently chosen from -(CHi) n -(CHi) n -, - (CHi) n NH(CHi) n -, -(CH 2 ) n O(CH 2 )n-, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH2) n -(CH2) n - and -(CH2) n O(CH2) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the invention provides a compound of Formula I:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyan
  • R8 is a -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyan
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
  • the invention provides a compound of Formula I where R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfin
  • the invention provides a compound of Formula I where R8 is -L- h ⁇ t ⁇ roaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sul
  • the invention provides a compound of Formula I where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfin
  • each L is independently chosen from -(CH 2 )I 1 -(CH 2 )I 1 -, - (CH 2 )nNH(CH 2 )n-, -(CH 2 )n0(CH 2 ) n -, and -(CH 2 ) n S(CH 2 )n-, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 )M-(CH 2 ),,- and -(CHi) n O(CH 2 )I 1 -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the invention provides a compound of Formula I where R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfon
  • the invention provides a compound of Formula I where R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfin
  • the invention provides a compound of Formula I where R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl
  • each L is independently chosen from -(CHa) n -(CHi) n -, -(CH2) n NH(CH2) n -, - (CH 2 ) n O(CH2) n -, and -(CH2) n S(CH2) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from - (CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or - CH 2 -.
  • L is a covalent bond.
  • the invention provides a compound of Formula I wherein R8 is heterocyclyl having from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heteroaryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulf
  • the invention provides compounds of Formula I wherein R6 and R7 are hydro.
  • the invention provides a compound of Formula I wherein R1 -R7 are each hydro and R8 is aryl group having from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, - L-aryl, -L-heteroaryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro,
  • the invention provides a compound of Formula I wherein the phenyl ring attached to the cyclopropyl ring has at least one substituent that is not hydro, i.e. at least one of Rl to R5 is not hydro.
  • the invention provides a method of treating a cancer comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier. Accordingly, in one embodiment, the invention provides a method for treating or preventing a cancer comprising the administration of a therapeutically effective amount of a compound of Formula I or a pharmaceutical composition comprising said compound and a pharmaceutically acceptable carrier to a subject (e.g. a human) in need of such a treatment or prevention. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer. In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for treating and/or preventing cancer.
  • the cancer is chosen from breast cancer, lung cancer, prostate cancer, testicular cancer, brain cancer, colorectal cancer, blood cancer (e.g., leukemia), or skin cancer.
  • the cancer is chosen from breast cancer, lung cancer, prostate cancer, testicular cancer, or brain cancer.
  • the invention provides a method of inhibiting LSD l activity comprising administering, to a patient in need of treatment, an amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier sufficient to inhibit LSDl activity.
  • the invention provides a compound of Formula I for inhibiting LSDl .
  • the invention provides for the use of a compound of Formula I for the manufacture of a medicament for inhibiting LSDl .
  • the invention provides a method of treating a neurodegenerative disease or disorder comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier. Accordingly, in one embodiment, the invention provides a method for treating or preventing a neurodegenerative disease comprising the administration of a therapeutically effective amount of a compound of Formula I or a pharmaceutical composition comprising said compound and a pharmaceutically acceptable carrier to a subject (e.g. a human) in need of such a treatment or prevention. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease.
  • the invention provides for the use of a compound of Formula I for the manufacture of a medicament for treating and/or preventing a neurodegenerative disease.
  • the neurodegenerative disease or disorder is chosen from Alzheimer's disease, Parkinson's disease, Huntington's disease, or Lewy Body dementia.
  • the invention provides a method of inhibiting monoamine oxidase activity comprising administering, to a patient in need of treatment, an amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier sufficient to inhibit monoamine oxidase activity.
  • the invention provides a compound of Formula I for treating and/or preventing Parkinson's disease and/or depression.
  • the invention provides for the use of a compound of Formula I for the manufacture of a medicament for inhibiting monoamine oxidase.
  • the monoamine oxidase is MAO-B.
  • the invention provides a method for identifying LSDl selective inhibitors comprising determining the ability of a test compound to inhibit LSDl and monoamine oxidase (MAO-A and/or MAO-B) wherein a test compound that inhibits LSD l better than monoamine oxidase (MAO-A and/or MAO-B) is LSD l selective.
  • the test compound is chosen from a phenylcyclopropylamine derivative, homolog or analog.
  • the invention provides a method for identifying a dual inhibitor selective for LSDl /MAO-B as compared to MAO-A comprising determining the ability of a test compound to inhibit LSDl and monoamine oxidase (MAO-A and MAO-B) wherein a test compound that inhibits LSDl and MAO-B better than LSDl and monoamine oxidase (MAO-A and/or MAO-B) is a dual LSDl /MAO-B selective inhibitor.
  • the test compound is chosen from a phenylcyclopropylamine derivative, homolog or analog.
  • Phenylcyclopropylamine derivatives, homologs, and analogs can be made by methods known in the art e.g., including, but not limited to the methods disclosed herein and in the references cited herein.
  • the present invention relates to compounds, the identification of compounds and their use for treating and/or preventing diseases.
  • the present invention provides compounds of Formula I, pharmaceutical compositions comprising a compound of Formula I and a pharmaceutically acceptable carrier, and their use for treating and/or preventing diseases.
  • One use of the compounds of Formula I is to treat and/or prevent cancer.
  • Compounds of the invention also inhibit monoamine oxidases, and can therefore be used for treating and/or preventing a disease in which monoamine oxidase inhibition is useful.
  • Some compounds of Formula I can be used as LSDl selective inhibitors that inhibit LSDl to a greater extent than MAO-A and/or MAO- B.
  • Some compounds of Formula I can be used as dual LSD1/MAO-B selective inhibitors that inhibit LSD l and MAO-B to a greater extent than MAO-A. Some of the compounds of Formula I can be used as inhibitors of MAO-A, MAO-B and LSDl .
  • phenylcyclopropylamine derivatives with monosubstitution on the amine group with ring bearing substituents yields compounds with unexpectedly potent LSDl inhibition.
  • some of the compounds of Formula I have IC50 values for LSDl inhibition of less than 1 micromolar (see Table 1 ) which makes them at least 20-30-fold more potent than tranylcypromine for LSDl inhibition.
  • these types of compounds also are potent inhibitors of the monoamine oxidases.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a compound of Formula I and a pharmaceutically acceptable carrier:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulf ⁇ nyl, sulfonyl, sulfonamido, thiocarbon
  • R6 is chosen from -H and alkyl
  • R7 is chosen from -H, alkyl, and cycloalkyl
  • R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyana
  • R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sul
  • R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, isocyanato, is
  • each L is independently chosen from -(CHa) n -(CH 2 ), ! -, - (CH 2 )nNH(CH 2 )n-, -(CH 2 )n0(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH2) n - and -(CH2) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryioxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl
  • R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, isocyanato, is
  • R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sul
  • each L is independently chosen from -(CH 2 )H-(CHa) n -, - (CH 2 )nNH(CH 2 )n-, -(CH 2 )n0(CH 2 )n-, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the R8 ring or ring system has 1 substituent
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 -3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
  • L is independently chosen from -(CH 2 V(CH 2 ),,-, -(CH 2 ) n NH(CH 2 V, -(CH 2 ) n O(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from O, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 V(CH 2 V an d -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from O, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I, as defined herein above, which is a selective inhibitor of LSD l .
  • LSDl selective inhibitors have IC50 values for LSDl which are at least 2-fold lower than the IC50 value for MAO-A and/or MAO-B.
  • the invention also provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I, as defined herein above, which is a dual inhibitor selective for LSD l and MAO-B.
  • Dual LSD l /MAO- B selective inhibitors have IC50 values for LSD l and MAO-B which are at least 2- fold lower than the IC50 value for MAO-A.
  • the invention provides a compound of Formula I for treating and/or preventing cancer:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbony
  • R6 is chosen from -H and alkyl
  • R7 is chosen from -H, alkyl, and cycloalkyl
  • R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyana
  • the R8 ring or ring system has from 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, aryl alkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - 5 -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 )n0(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from O, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the cancer is prostate cancer. In another specific aspect of this embodiment, the cancer is brain cancer. In yet another specific aspect of this embodiment, the cancer is breast cancer. In yet another specific aspect of this embodiment, the cancer is lung cancer. In yet another specific aspect of this embodiment, the cancer is testicular cancer. In yet another specific aspect of this embodiment, the cancer is colorectal cancer. In yet another specific aspect of this embodiment, the cancer is blood cancer (e.g., leukemia). In yet another specific aspect of this embodiment, the cancer is skin cancer.
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, isothiocyana
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro,
  • the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, isothiocyanato,
  • each L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 )n-, -(CH 2 ) n O(CH 2 )n-, and -(CFb) n S(CLb) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH2) n -(CH2) n - and - (CFb) n O(CFb) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the R8 ring or ring system has 1 substituent chosen from hydroxyl,
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
  • L is independently chosen from -(CH 2 ) n -(CH2) n -, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) n 0(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from O, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 )n0(CH 2 ) n -, where each n is independently chosen from O, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a compound of Formula I for treating and/or preventing cancer, wherein R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro,
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, isothiocyanato,
  • each L is independently chosen from -(CHi) n -(CH 2 ),!-, - (CH 2 )nNH(CH 2 ) n -, -(CH 2 )n0(CH 2 )n-, and -(CH 2 ) n S(CH 2 )n-, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 )n0(CH2) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the invention provides a compound of Formula I for treating and/or preventing cancer, wherein R8 is -L-aryl wherein said aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, haloaryl,
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is aryl (L is a bond) wherein said aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, is
  • the invention provides a compound of Formula I for treating and/or preventing cancer
  • R8 is a phenyl group wherein said phenyl has from 1-3 (i.e. 1, 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, is
  • each L is independently chosen from -(CH 2 )I 1 -(CHi) n -, - (CH 2 ) n NH(CH 2 )n-, -(CH 2 ) n O(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CHi) n O(CHi) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CHi-.
  • L is a covalent bond.
  • the R8 ring or ring system has from 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) ⁇ O(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CHa) n O(CHi) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined in the above described embodiment.
  • the invention provides a compound of Formula I for use in treating and/or preventing cancer:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbon
  • R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfiny
  • R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl,
  • R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyioxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro,
  • R8 is -L-heterocyclyl wherein said -L-heterocyclyl is -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylallal
  • R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents and further wherein said ring or ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, oxadiazolyl, 2,3-dihydrobenzofuranyl, benzo[d][l ,3]dioxolyl,
  • R8 is a -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents and further wherein said ring or ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, and oxadiazolyl.
  • R8 is a -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents and further wherein said ring or ring system is pyridyl.
  • R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro
  • R8 is aryl (L is a bond) wherein said aryl has from 1-3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl
  • L is independently chosen from -(CH 2 ) n -(CH2) n - and -(CH2) n O(CH 2 ) n -, wherein each n is independently chosen from 0, 1 , 2, and 3.
  • L is -CH 2 - or a covalent bond.
  • L is a covalent bond.
  • R6 and R7 are hydro.
  • At least one of Rl to R5 is not hydro.
  • one of R1-R5 is chosen from -L-aryl, -L-heterocyclyl, and -L-carbocyclyl.
  • the R8 ring or ring system has 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
  • the cancer to be treated and/or prevented is chosen from breast cancer, lung cancer, prostate cancer, testicular cancer, brain cancer, colorectal cancer, blood cancer, and skin cancer.
  • the invention provides a compound of Formula I:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbony
  • R8 is a -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothio
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined in the above described embodiment.
  • the invention provides a compound of Formula I where R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfin
  • the invention provides a compound of Formula I where R8 is -L- heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl,
  • the invention provides a compound of Formula I where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfin
  • each L is independently chosen from -(CHi) n -(CHi) n -, - (CH 2 )nNH(CH 2 ) n -, -(CHi) n O(CHi) n -, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CHi) n -(CHi) n - and -(CHi) n O(CHi) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CHi-.
  • L is a covalent bond.
  • the invention provides a compound of Formula I where R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfon
  • the invention provides a compound of Formula I where R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfin
  • the invention provides a compound of Formula I where R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl
  • each L is independently chosen from -(CH 2 )I 1 -(CHi) n -, - (CH 2 )nNH(CH 2 )n-, -(CH 2 ) n O(CH 2 )n-, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the invention provides a compound of Formula I where R8 is -L-aryl wherein said aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, isothiocyanato
  • the invention provides a compound of Formula I where R8 is aryl (L is a bond) wherein said aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyan
  • the invention provides a compound of Formula I where R8 is a phenyl group wherein said phenyl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyana
  • each L is independently chosen from -(CH 2 )Ii-(CH 2 )I 1 -, - (CH 2 )nNH(CH 2 )n-, -(CH 2 ) n O(CH 2 )n-, and -(CH 2 ) n S(CH 2 ) n -, and where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 )n-(CH 2 ) n - and -(CH 2 ) n O(CH2) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -.
  • L is a covalent bond.
  • the R8 ring or ring system has 1 substituent chosen from hydroxyl
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • RJ is chosen from -H and cycloalkyl.
  • R7 is -H.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) n O(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from O, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 )n-(CH2) n - and -(CH 2 ) n O(CH 2 )n-, where each n is independently chosen from O, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a compound of Formula I:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino.
  • R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl.
  • R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl
  • R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl,
  • Rl , R2, R3, R4, R5, and R7 are all hydro, L is a bond and R8 is phenyl, then R6 is not methyl, ethyl, or isopentyl.
  • L is independently chosen from -(CHi) n -(CHi) n - and -(CHi) n O(CHi) n -, wherein each n is independently chosen from 0, 1 , 2, and 3.
  • L is -CHi- or a covalent bond.
  • L is a covalent bond.
  • R6 and R7 are each hydro.
  • R8 is an -L-heterocycyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro,
  • R8 is an -L-heterocyclyl wherein said -L-heterocycyl is a -L-heteroaryl and wherein the ring or ring system of said - L-heteroaryl has from 0-3 substituents chosen from halo, alky], alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy,
  • R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents and further wherein said ring or ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, oxadiazolyl, 2,3-dihydrobenzofuranyl, benzo[d] [l ,3]dioxolyl,
  • R8 is a -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents and further wherein said ring or ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, and oxadiazolyl.
  • R8 is a -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents and further wherein said ring or ring system is pyridyl.
  • R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyi, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sul
  • R8 is an -L-aryl that has 3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyi, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl
  • one of Rl , R2, R3, R4, and R5 is not a hydro.
  • one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl.
  • the R8 ring or ring system has 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
  • the invention provides a compound of Formula I for treating and/or preventing cancer:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heteroaryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulf
  • R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothi
  • the R8 ring or ring system has 1 substituent chosen from hydroxy
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 -3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C 1 -3 alkyl) 2 , -NH(Cj -3 alkyl), -C(O)NH 2 , - C(O)NH(C 1-3 alkyl), -C(O)N(C 1-3 alkyl) 2 , -S(O) 2 (C 1-3 alkyl), -S(O) 2 NH 2 , - S(O) 2 N(C 1 -3 alkyl) 2 , -S(O) 2 NH(C 1 -3 alkyl), -CHF 2 , -OCF 3 , -OCHF 2 , -CF 3 , -CN, - NH 2 , and -NO 2 .
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H. In one aspect of this embodiment, L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) n O(CH 2 )n-, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH2) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfiny
  • R6 is chosen from -H and alkyl
  • R7 is chosen from -H, alkyl, and cycloalkyl
  • R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothi
  • each L is independently chosen from -(CH 2 ) n -(CH2) n -, -(CH2) n NH(CH 2 ) n -, -
  • R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl, 4- methoxyphenyl, 4-chlorophenyl or furan-2-yl, and also when Rl , R2, R3, R4, R5, and R7 are all hydro, R8 is phenyl and L is a bond, then R6 is not methyl, ethyl or isopentyl.
  • the invention provides a pharmaceutical composition
  • the R8 ring or ring system has 1 substituent chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Cj -3 alkyl) 2 , - NH(Ci -3 alkyl), -C(O)NH 2 , -C(O)NH(Ci -3 alkyl), - C(O)N(C 1 -3 alkyl) 2 , - S(O) 2 (C 1-3 alkyl), -S(O) 2 NH 2 , -S(O) 2 N(Ci -3 alkyl) 2 , - S(O) 2 NH(C 1 -3 alkyl), - CHF 2 , -OCF 3 , -OCHF 2 , -CF 3 , -CN, -NH 2 , and -NO 2 .
  • the R8 ring or ring system has 1 substituent chosen from hydroxyl
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) n O(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n - 5 where each n is independently chosen from O, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a compound of Formula I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkyl amino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfmyl, sulfonyl, sulfonamido
  • each L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH2) n -, - (CH 2 ) n O(CH 2 ) n -, and -(CH2) n S(CH 2 )n-, and where each n is independently chosen from O, 1 , 2, and 3 ; and pharmaceutically acceptable salts thereof; with the provision that when Rl , R2,
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
  • R6 and R7 are each hydro.
  • Rl, R2, R3, R4, R5, R6, and R7 are each hydro.
  • R8 is a heteroaryl having from 0-3 substituents.
  • the heteroaryl ring is chosen from thiazolyl, pyridyl, thiophenyl, and quinolinyl.
  • R8 is a heteroaryl that has from 1 -3 substituents independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl. In one aspect of this embodiment, R8 is not an unsubstituted furan-2-yl or phenyl group when L is a bond.
  • the invention provides a compound of Formula I wherein Rl , R2, R3, R4, R5, R6 and R7 are each hydro and R8 is an -L- aryl group having from 0 to 3 substituents on the ring or ring system of said -L-aryl, the substituents being independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl,
  • R8 is a phenyl group having from 1 to 3 substituents.
  • the substituents on the R8 phenyl ring are chosen from halo, alkyl, alkoxy, cycloalkoxy, cyano, haloalkyl and hydroxyl.
  • the substituents on the R8 ring are chosen from -F, -Cl, -Br, -CH 3 , -OH, -
  • Each L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 ) n -, -
  • the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
  • the invention provides a compound of Formula I wherein: one of R1 -R5 is chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido,
  • R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothi
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, aryl alkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) n 0(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 )I 1 -(CHi) n - and -(CHi) n O(CHi) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a compound of Formula I wherein one of R1-R5 is a substituent chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfon
  • R8 is chosen from aryl or heterocyclyl wherein the ring or ring system of said aryl or heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, s
  • the ring or ring system of R8 has 1 -3 substituents as described and defined above. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents as defined above. In an even more specific aspect, the ring or ring system of R8 has 1 substituent as defined above.
  • the one of R1-R5 is chosen from -L-aryl, -L-heterocyclyl, and -L-carbocyclyl. In an even more specific aspect, the one of Rl- R5 is -L-aryl, In an even more specific aspect, the one of R1 -R5 is benzyloxy.
  • the substituent(s) on R8 are independently chosen from halo, alkyl, alkoxy, cycloalkoxy, cyano, haloalkyl, and hydroxyl.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
  • the invention provides a compound of Formula I wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfon
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) n O(CH 2 ) n -, and -(CH 2 )nS(CH 2 )n-, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 -3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • the invention provides a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from halo, alkoxy, cycloalkoxy, cyano, and alkyl.
  • the R8 ring or ring system has 1 or 2 substituents independently chosen from halo, alkoxy, cycloalkoxy, cyano, and alkyl.
  • the R8 ring or ring system has 1 substituent independently chosen from halo, alkoxy, cyano, and alkyl.
  • the invention provides a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from -Cl, -Br, -F, cyano, and methoxy.
  • the R8 ring or ring system has 1 or 2 substituents indepependently chosen from -Cl, -Br, -F, cyano, and methoxy.
  • the R8 ring or ring system has 1 substituent independently chosen from -Cl, -Br, -F, cyano, and methoxy.
  • the invention provides a compound of Formula I wherein R8 is chosen from phenyl, thiazolyl, pyridyl, thiophenyl. and quinolinyl.
  • the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
  • the invention provides compounds of Formula I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido
  • R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothi
  • the ring or ring system of R8 has 1-3 substituents as described and defined above. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents as defined above. In an even more specific aspect, the ring or ring system of R8 has 1 substituent as defined above.
  • the invention provides a compound of Formula I wherein R8 is aryl wherein the ring or ring system of said aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulf
  • the invention provides a compound of Formula I wherein R8 is heteroaryl wherein the ring or ring system of said heteroaryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulf
  • the invention provides a compound of Formula I wherein R8 is heterocyclyl wherein the ring or ring system of said heterocyclyl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, s
  • the invention provides a compound of Formula I wherein R8 is chosen from phenyl, pyridinyl, thiazolyl, and thiophenyl, wherein the ring of said group has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, iso
  • the invention provides a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from halo, alkoxy, cycloalkoxy, cyano, and alkyl.
  • the R8 ring or ring system has 1 or 2 substituents independently chosen from halo, alkoxy, cycloalkoxy, cyano, and alkyl.
  • the R8 ring or ring system has 1 substituent chosen from halo, alkoxy, cyano, and alkyl.
  • the invention provides a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from -Cl, -Br, -F, cyano, and methoxy.
  • the R8 ring or ring system has 1 or 2 substituents independently chosen from -Cl, -Br, -F, cyano, and methoxy.
  • the R8 ring or ring system has 1 substituent chosen from -Cl, -Br, -F, cyano, and methoxy.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
  • the invention provides a compound of Formula I wherein Rl , R2, R3, R4, and R5 are each hydro; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
  • R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, aryl alkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothi
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n -, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) n O(CH 2 )n-, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from O, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from O, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
  • the invention provides a compound of Formula I wherein one of Rl , R2, R3, R4, and R5 is chosen from halo, alkyl, alkoxyl, haloalkyl, haloalkoxy, cyano, amino, alkylamino, -L-heterocyclyl, -L-aryl, and -L-carbocyclyl; and the others of Rl, R2, R3, R4, and R5 are chosen from -H, halo, alkyl, alkoxyl, haloalkyl, haloalkoxy, cyano, amino, alkylamino, -L-het ⁇ rocyclyl, -L-aryl, and -L- carbocyclyl;
  • R6 is chosen from -H and alkyl
  • R7 is chosen from -H, alkyl, and cycloalkyl
  • R8 is chosen from heteroaryl and aryl, wherein the ring or ring system of said heteroaryl or aryl has from 0-3 substituent independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heteroaryl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulf
  • R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
  • the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 -3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent.
  • the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, s
  • L is independently chosen from -(CH 2 V(CH 2 V, -(CH 2 ) n NH(CH 2 ) n -, -(CH 2 ) n 0(CH 2 ) n -, and -(CH 2 ) n S(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is independently chosen from -(CH 2 ) n -(CH 2 ) n - and -(CH 2 ) n O(CH 2 ) n -, where each n is independently chosen from 0, 1 , 2, and 3.
  • L is chosen from a covalent bond or -CH 2 -. In an even more specific aspect, L is a covalent bond.
  • the invention provides a compound of Formula I wherein one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, - L-aryl, and -L-carbocyclyl.
  • one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl, and the others are hydro.
  • L is a covalent bond.
  • the invention provides a compound of Formula I wherein R6 and R7 are hydro, and one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl.
  • R6 and R7 are hydro
  • one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl
  • the others of Rl to R5 are hydro.
  • L is a covalent bond.
  • the invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
  • the ring or ring system of R8 has at least one substituent chosen from the substituents described and defined in the respective embodiments.
  • the ring or ring system of R8 has three substituents chosen from the substituents described and defined in the respective embodiments.
  • said three substituents may be independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, isothiocyanato, isothiocyan
  • R8 is aryl (such as, e.g., phenyl) or heterocyclyl (such as, e.g., pyridinyl, thiazolyl, or thiophenyl), wherein the ring or ring system of said aryl has 1 -3 substituents (such as, e.g., 1 or 2 substituents) and further wherein the ring or ring system of said heterocyclyl has from 0-3 substituents (such as, e.g., 1 or 2 substituents), said substituents being independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, - L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkyl
  • said substituents are independently chosen from halo, alkyl, alkoxy,
  • one of R1 -R5 is chosen from -L-aryl, -L-heterocyclyl, and -L-carbocyclyl. In a more specific aspect, one of R1-R5 is -L-aryl. In an even more specific aspect, one of R1 -R5 is benzyloxy.
  • R3 is benzyloxy and each of Rl , R2, R4 and R5 is hydro.
  • the ring or ring system of R8 is a heterocycyl
  • said ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, oxadiazolyl, 2,3-dihydrobenzofuranyl, benzo[d] [l ,3]dioxolyl, 2,3-dihydrobenzo[b] [l ,4]dioxinyl, 3,4-dihydro-2H- benzo[b] [l ,4]dioxepinyl, and chromanyl.
  • ring or ring system of R8 when the ring or ring system of R8 is a heteroaryl said ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, and oxadiazolyl. In an even more preferred aspect R8 is pyridyl.
  • the compound of Formula I is chosen from:
  • the invention provides a compound of Formula I chosen from the above compounds, in particular for use as a medicament and also for use in treating and/or preventing cancer.
  • the compound of Formula I is chosen from:
  • the invention provides a compound of Formula I chosen from the above compounds, in particular for use as a medicament and also for use in treating and/or preventing cancer.
  • LSDl selective inhibitors have IC50 values for LSDl which are at least 2- fold lower than the IC50 value for MAO-A and/or MAO-B. In some embodiments, the LSDl selective inhibitors have IC50 values which are at least 5-fold lower for LSDl as compared to MAO-A and MAO-B. In some embodiments, the LSDl selective inhibitors have IC50 values which are at least 10-fold lower for LSDl as compared to MAO-A and MAO-B.
  • the invention also provides compounds of Formula I which are dual inhibitors selective for LSDl and MAO-B.
  • Dual LSD1/MA0-B selective inhibitors have IC50 values for LSDl and MAO-B which are at least 2-fold lower than the IC50 value for MAO-A.
  • the dual LSD 1/MA0-B selective inhibitors have IC50 values which are at least 5-fold lower for LSDl and MAO-B as compared to MAO-A.
  • the dual LSD1/MA0-B selective inhibitors have IC50 values which are at least 10-fold lower for LSDl and MAO-B as compared to MAO-A.
  • the LSD1/MA0-B inhibitor avoids the deleterious side-effects asscoaited with inhibition of MAO-A.
  • the invention provides a method of treating a cancer comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier.
  • the invention provides a compound of Formula I for treating and/or preventing cancer.
  • the invention provides for the use of a compound of Formula I for the manufacture of a medicament for treating and/or preventing cancer.
  • Egr-1 is a tumor suppressor gene in many contexts (see e.g., Calogero el al. (2004) Cancer Cell International 4: 1 exogenous expression of EGR-1 resulted in growth arrest and eventual cell death in primary cancer cell lines; Lucerna et al. (2006) Cancer Research 66, 6708-6713 show that sustained expression of Egr-1 causes antiangiogeneic effects and inhibits tumor growth in some models; Ferraro et al. ((2005) J. Clin. Oncol.
  • the instant inventors have discovered a class of LSDl inhibitors that can be used to treat diseases where LSDl is implicated as a therpautic target like cancer. Accordingly, the phenylcyclopropylamine compounds of the invention can be used to treat and/or prevent such diseases.
  • the compounds disclosed herein are surprisingly and significantly more potent than tranylcypromine for LSDl inhibition.
  • Han et al. Euro. J. Pharma. (2008) doi: 10.1016/j.ejphar.2008.12.025
  • the compounds of Formula I may be used as a neuroprotectant (e.g., used to treat and/or prevent conditions characterized by neurodegeneration).
  • the compounds of Formula I are potent LSDl inhibitor they can be used to treat and/or prevent diseases where LSDl inhibition is desirable, e.g., cancer.
  • dual MAO-B/LSD1 inhibitors provided herein are thought of interfering with a mechanism involved in neurodegenerative diseases. Accordingly, compounds that potently inhibit both MAO-B and LSDl activity are preferred in the treatment of neurodegenerative diseases.
  • Exemplary compounds in this context are shown in Table 1 , wherein compounds with a low IC50 value (in particular compounds having a lower IC50 value than the prior art compound parnate) are preferred.
  • compounds that inhibit both MAO A/B acitivy and LSDl activity are envisaged herein in the treatment of diseases, in particular neurodegenerative diseases, wherein compounds that inhibit LSDl activity more potently than parnate are preferred.
  • the invention provides a method of inhibiting LSD l activity comprising administering, to a patient in need of treatment, an amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier sufficient to inhibit LSDl activity.
  • the invention provides a compound of Formula I for inhibiting LSDl .
  • the invention provides for the use of a compound of Formula I for the manufacture of a medicament for inhibiting LSDl .
  • the invention provides a method of treating a neurodegenerative disease or disorder comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier.
  • the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease.
  • the invention provides for the use of a compound of Formula I for the manufacture of a medicament for treating and/or preventing a neurodegenerative disease.
  • the invention provides a method of inhibiting monoamine oxidase activity comprising administering, to a patient in need of treatment, an amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier sufficient to inhibit monoamine oxidase activity.
  • the invention provides a compound of Formula I for treating and/or preventing Parkinson's disease and/or depression.
  • the invention provides for the use of a compound of Formula I for the manufacture of a medicament for inhibiting monoamine oxidase.
  • the invention provides a compound of Formula I for treating and/or preventing neurogeneration..
  • the compound does not have the structure of the compounds having CAS registration nos.
  • the invention provides a method of treating a disease or condition comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier.
  • the disease is cancer or a neurodegenerative disease.
  • the invention provides a method of treating a cancer comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier.
  • the cancer is prostate cancer.
  • the cancer is brain cancer.
  • the cancer is breast cancer.
  • the cancer is lung cancer.
  • the cancer is testicular cancer.
  • the cancer is colorectal cancer.
  • the cancer is blood cancer (e.g., leukemia).
  • the cancer is skin cancer.
  • the invention provides a method of treating a neurodegenerative disease or disorder comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier.
  • the invention provides a method for identifying LSDl selective inhibitors comprising determining the ability of a test compound to inhibit LSDl and monoamine oxidase (MAO-A and/or MAO-B) wherein a test compound that inhibits LSD l better than monoamine oxidase (MAO-A and/or MAO-B) is LSD l selective.
  • the selective inhibitor has an IC50 value at least two-fold lower for LSDl as compared to MAO-A and MAO-B.
  • the LSD l selective inhibitor has an IC50 value at least five-fold lower for LSDl as compared to MAO-A and MAO-B.
  • the LSDl selective inhibitor has an IC50 value at least ten-fold lower for LSD l as compared to MAO-A and MAO-B.
  • the test compound is chosen from a phenylcyclopropylamine derivative, homolog or analog.
  • the phenylcyclopropylamine analog has the phenyl group replaced with another ring system (e.g., aryl, heterocyclyl and/or heteroaryl which is optionally substituted) and the amine group is substituted with a functional group (see e.g., examples).
  • the test compound is a phenylcyclopropylamine analog or derivative where the amine group is substituted with a functional group.
  • the test compound is a phenylcyclopropylamine analog or derivative where the amine group is substituted with a functional group and the phenyl group has at least one substituent that is not a hydrogen atom.
  • the invention provides a method of inhibiting LSDl selectively as compared to MAO-B and MAO-A comprising administering to an individual a phenylcyclopropylamine derivative, homolog, or analog that selectively inhibits LSDl .
  • the invention provides a method of inhibiting LSDl selectively as compared to MAO-B and MAO- A comprising administering to an individual a compound of Formula I as defined in the embodiments described in this invention, that selectively inhibits LSDl .
  • the invention provides a method for identifying a dual inhibitor selective for LSDl /MAO-B as compared to MAO-A comprising determining the ability of a test compound to inhibit LSDl and monoamine oxidase (MAO-A and MAO-B) wherein a test compound that inhibits LSDl and MAO-B better than LSDl and monoamine oxidase (MAO-A and/or MAO-B) is a dual LSDl /MAO-B selective inhibitor.
  • the dual LSDl /MAO-B selective inhibitor has an IC50 value at-least two-fold lower for LSDl and MAO-B as compared to MAO-A.
  • the dual LSDl /MAO-B selective inhibitor has an IC50 value at-least five-fold lower for LSDl and MAO-B. In one aspect of this embodiment, the dual LSD 1/MA0-B selective inhibitor has an IC50 value at-least ten-fold lower for LSD l and MAO-B as compared to MAO-A. In one aspect of this embodiment, the test compound is chosen from a phenylcyclopropylamine derivative, homolog or analog.
  • the phenylcyclopropylamine analog has the phenyl group replaced with another ring system (e.g., aryl, heterocyclyl and/or heteroaryl which is optionally substituted) and the amine group is substituted with a functional group (see e.g., examples).
  • the test compound is a phenylcyclopropylamine analog or derivative where the amine group is substituted with a functional group.
  • the test compound is a phenylcyclopropylamine analog or derivative where the amine group is substituted with a functional group and the phenyl group has at-least one substituent that is not a hydrogen.
  • the invention provides a method of inhibiting LSD l and MAO-B selectively as compared to MAO-A comprising administering to an individual a phenylcyclopropylamine derivative, homolog, or analog that selectively inhibits LSDl and MAO-B.
  • the invention provides a method of inhibiting LSDl and MAO-B selectively as compared to MAO- A comprising administering to an individual a compound of Formula I as defined in the embodiments described in this invention, and that selectively inhibits LSDl and MAO-B.
  • Phenylcyclopropylamine derivatives, homologs, and analogs can be made by methods known in the art e.g., including, but not limited to the methods disclosed herein and in the references cited herein. See for example, Kaiser et al. (1962) J. Med. Chem. 5: 1243-1265 and Zirkle et al. (1962) J. Med. Chem. 1265-1284 Yoshida et al. (2004) Bioorg. Med Chem. 12(10):2645-2652; Hruschka et al. (2008) Biorg Med Chem. (16):7148-7166; and Gooden et al. (2008) Bioorg. Med. Chem. Let.
  • the invention provides a method of identifying LSD l selective and LSD1/MAO-B inhibitors comprising determining the ability of a test compound to inhibit LSDl , MAO-A, and MAO-B wherein a LSDl selective inhibitor is identified when the test compound inhibits LSDl to a greater extent than MAO-A and MAO-B and wherein a LSDl /MAO-B dual inhibitor is identified when the test compound inhibits LSDl and MAO-B to a greater extent than it inhibits MAO-A wherein said test compound is compound of Formula I:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl
  • R6 is chosen from -H and alkyl
  • R7 is chosen from -H, alkyl, and cycloalkyl
  • R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyana
  • LSDl selective and LSDl /MAO-B dual inhibitors can be used to prepare pharmaceutical compositions for treating diseases according to the methods of the invention as described herein.
  • the diseases can be treated by inhibiting LSDl , LSDl and MAO-B, and/or by modulating histone methylation levels.
  • the invention provides a method of identifying LSDl selective and LSDl /MAO-B dual inhibitors comprising determining the ability of a test compound to inhibit LSD l , MAO-A, and MAO-B wherein a LSDl selective inhibitor is identified when the test compound inhibits LSDl to a greater extent than MAO-A and MAO-B and wherein a LSDl /MAO-B dual inhibitor is identified when the test compound inhibits LSDl and MAO-B to a greater extent than it inhibits MAO-A wherein said test compound is compound of Formula I:
  • each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbon
  • R7 is chosen from -H, alkyl, and cycloalkyl
  • R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfiny
  • R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl
  • alkyl refers to a saturated aliphatic hydrocarbon including straight chain and/or branched chain groups.
  • the alkyl group is further defined as having 1 to 20 carbon atoms (whenever it appears herein, a numerical range such as “ 1 to 20" refers to each integer in the given range; e.g., " 1 to 20 carbon atoms” means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc. up to and including 20 carbon atoms).
  • it is an alkyl having 1 to 10 carbon atoms.
  • it is an alkyl having 1 to 6 carbon atoms
  • it is an alkyl having 1 to 4 carbon atoms.
  • alkenyl refers to an unsaturated hydrocarbon including straight chain and/or branched chain groups, comprising at least one carbon-to- carbon double bond.
  • the alkenyl group is further defined as having 2 to 20 carbon atoms.
  • it is an alkenyl having 2 to 10 carbon atoms.
  • it is an alkenyl having 2 to 6 carbon atoms, and in yet another more specific definition, it is an alkenyl having 2 to 4 carbon atoms.
  • alkynyl refers to an unsaturated hydrocarbon including straight chain and/or branched chain groups, comprising at least one carbon-to- carbon triple bond.
  • the alkynyl group is further defined as having 2 to 20 carbon atoms.
  • it is an alkynyl having 2 to 10 carbon atoms.
  • it is an alkynyl having 2 to 6 carbon atoms, and in yet another more specific definition, it is an alkynyl having 2 to 4 carbon atoms.
  • halo refers to a group chosen from chloro, fluoro, bromo, and iodo.
  • hydro refers to a hydrogen atom (-H group) which is also denoted as -H.
  • hydroxyl refers to an -OH group.
  • alkoxy refers to an -O-alkyl group, wherein the alkyl group is as defined herein above.
  • cycloalkoxy refers to an -O-cycloalkyl group, wherein the cycloalkyl group is as defined herein below.
  • aryloxy refers to an -O-aryl group, wherein the aryl group is as defined herein below.
  • heteroaryloxy refers to an -O-heteroaryl group, wherein the heteroaryl group is as defined herein below.
  • mercapto refers to an -SH group.
  • alkylthio refers to an -S-alkyl group, wherein the alkyl group is as defined herein above.
  • cycloalkylthio refers to an -S-cycloalkyl group, wherein the cycloalkyl group is as defined herein below.
  • arylthio refers to an -S-aryl group, wherein the aryl group is as defined herein below.
  • heteroarylthio refers to an -S-heteroaryl group, wherein the heteroaryl group is as defined herein below.
  • R is selected from the group consisting of hydro, alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon atom) and heterocyclyl (bonded through a ring carbon atom), wherein said hydro, alkyl, cycloalkyl, aryl, heteroaryl and heterocyclyl are as defined herein.
  • aldehyde refers to a carbonyl group as defined herein above, wherein R" is hydro.
  • esters refers to a C-carboxy group as defined herein above or a molecule comprising such group, wherein R" is as defined herein above and R" is not hydro.
  • M + group or a molecule comprising such group, wherein r is 1 to 6 and M + is selected from the group consisting of lithium, sodium, potassium, calcium, magnesium, barium, iron, zinc and quaternary ammonium, and further wherein the " + " in M + does not reflect the actual number of positive charges which depends on the respective ion.
  • carboxylic acid refers to a C-carboxy group as defined herein above, wherein R" is hydro.
  • haloalkyl refers to an alkyl group substituted with 1 to 6 halo groups, wherein the alkyl group and the halo groups are as defined herein above, and further wherein the halo groups are independently selected.
  • haloalkyl is a -CX 3 group, wherein each X independently is a halo group.
  • cyano refers to a -C ⁇ N group.
  • cyanato refers to an -OCN group.
  • isocyanato refers to an -NCO group.
  • thiocyanato refers to an -SCN group.
  • isothiocyanato refers to an -NCS group.
  • Rn and Ri 8 are independently chosen from hydro, alkyl, aryl, carbocyclyl, heterocyclyl, -(CH 2 )aryl, -(CH 2 )carbocyclyl, and (CH 2 )heterocyclyl, wherein hydro, alkyl, aryl, carbocyclyl and heterocyclyl are as defined herein.
  • O-carbamyl refers to an group, wherein Rn and Ri 8 are as defined herein above.
  • amino refers to an -NH 2 group.
  • alkylamino refers to an -NR 23 R 24 group, wherein R 23 and R 24 are independently chosen from -H, Ci -8 alkyl (i.e., an alkyl having 1 to 8 carbon atoms), and phenyl.
  • nitro refers to an -NO 2 group.
  • quaternary ammonium refers to an -NR2oR2]R-22 group, wherein R 2 o, R21, and R22 are independently selected from the group consisting of hydro and Cj -6 alkyl.
  • methylenedioxy refers to an -OCH 2 O- group, wherein the two oxygen atoms are bonded to adjacent ring carbon atoms.
  • ethylenedioxy refers to an -OCH 2 CH 2 O- group, wherein the two oxygen atoms are bonded to adjacent ring carbon atoms.
  • the term "carbocyclyl” refers to an all-carbon monocyclic or fused ring (i.e., rings which share an adjacent pair of ring carbon atoms) group, wherein one or more of the rings does not have a completely conjugated pi-electron system. In a more specific definition, it refers to a cycloalkyl group having 3 to 12 carbon atoms or a cycloalkenyl group having 3 to 12 carbon atoms. In another more specific definition, it refers to a cycloalkyl group having 3 to 6 carbon atoms or a cycloalkenyl group having 3 to 6 carbon atoms.
  • Examples, without limitation, of carbocyclyl groups are cycloalkyls such as cyclopropane, cyclobutane, cyclopentane, cyclohexane, adamantane, cycloheptane and cycloalkenes such as cycloheptatriene, cyclopentene, and cyclohexadiene.
  • cycloalkyl refers to an all-carbon monocyclic or fused ring (i.e., rings which share an adjacent pair of ring carbon atoms) group, wherein said monocyclic or fused ring group does not have a double or triple bond. In a more specific definition, it refers to a C 3 . 12 cycloalkyl group, i.e., an all-carbon monocyclic or fused ring group having 3 to 12 carbon atoms, wherein said monocyclic or fused ring group does not have a double or triple bond.
  • cycloalkyl group i.e., an all-carbon monocyclic or fused ring group having 3 to 6 carbon atoms, wherein said monocyclic or fused ring group does not have a double or triple bond.
  • cycloalkyl groups are cyclopropane, cyclobutane, cyclopentane, cyclohexane, adamantane, and cycloheptane.
  • heterocyclyl refers to a saturated or partially saturated monocyclic or fused-ring polycyclic group having 3 to 14 ring atoms, said ring atoms comprising carbon atoms and from one to four heteroatoms independently selected from the group consisting of O, N, and S, wherein the nitrogen and sulfur heteroatoms can be optionally oxidized, the nitrogen heteroatoms can be optionally quaternized, and including any bicyclic group in which any of the above-defined heterocyclic rings is fused to a benzene ring.
  • heterocyclyl thus also include heteroaryl groups as defined herein below.
  • heterocyclyl refers to a saturated or partially saturated 3- 7 membered monocyclic, or 7-10 membered bicyclic ring system, which consists of carbon atoms and from one to four heteroatoms independently selected from the group consisting of O, N, and S, wherein the nitrogen and sulfur heteroatoms can be optionally oxidized, the nitrogen heteroatoms can be optionally quaternized, and including any bicyclic group in which any of the above-defined heterocyclic rings is fused to a benzene ring.
  • Non-limiting examples of saturated or partially saturated heterocyclyl groups include tetrahydrofuranyl, pyranyl, piperidinyl, piperazinyl, pyrrolidinyl, imidazolidinyl, imidazolinyl, indolinyl, isoindolinyl, quinuclidinyl, morpholinyl, isochromanyl, chromanyl, pyrazolidinyl, pyrazolinyl, tetronoyl and tetramoyl groups.
  • heterocycles or “heterocyclic” rings also include, but are not limited to, morpholino, piperidyl, piperazinyl, pyrrolidinyl, thiomorpholino, homopiperazinyl, imidazolyl, imidazolidinyl, pyrazolidinyl, dioxanyl and dioxolanyl.
  • aryl refers to an all-carbon monocyclic or fused-ring polycyclic (i.e., rings which share an adjacent pair of ring carbon atoms) aromatic group having a completely conjugated pi-electron system. Examples, without limitation, of aryl groups are phenyl, naphthalenyl and anthracenyl.
  • heteroaryl refers to a monocyclic or fused-ring polycyclic group having 5 to 14 ring atoms; having 6, 10 or 14 pi electrons shared in a cyclic array; and containing carbon atoms and 1, 2 or 3 heteroatoms independently selected from the group consisting of O, N, and S. In a more specific definition, it refers to a monocyclic or fused-ring polycyclic aromatic group having 5 to 9 ring atoms and comprising 1 , 2 or 3 heteroatoms independently selected from the group consisting of O, N, and S.
  • heteroaryl groups include thienyl (thiophenyl), benzo[b]thienyl, naphtho[2,3-b]thienyl, thianthrenyl, furyl (furanyl), isobenzofuranyl, chromenyl, xanthenyl, phenoxanthiinyl, pyrrolyl (including, without limitation, 2H-pyrrolyl), imidazolyl, pyrazolyl, pyridyl (pyridinyl; including, without limitation, 2 -pyridyl, 3 -pyridyl, and 4-pyridyl), pyrazinyl, pyrimidinyl, pyridazinyl, indolizinyl, isoindolyl, indolyl (including, without limitation, 3H-indolyl), indazolyl, purinyl, 4H-quinolizinyl, isoquinolyl, quinolyl, quinolyl
  • nitrogen atom may optionally be in the form of an N-oxide, e.g., a pyridyl N-oxide, pyrazinyl N-oxide and pyrimidinyl N-oxide.
  • substituents covalently linked to the parent group, wherein said substituents are independently chosen from halo, alkyl, alkynyl, alkenyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-carbocyclyl, -L- aryl, -L-heteroaryl, -L-heterocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, is
  • arylalkyl refers to a C M O alkyl group (an alkyl group having 1 -10 carbon atoms), as defined herein above, substituted by a C 6-I4 aryl group (an aryl group having 6 to 14 carbon atoms), as defined herein above.
  • arylalkyl groups include benzyl, phenethyl, and naphthylmethyl.
  • arylalkenyl refers to a C 2 . io alkenyl group substituted by a C 6-14 aryl group (an aryl group having 6 to 14 carbon atoms), as defined herein above.
  • arylalkynyl refers to a C 2 . 10 alkynyl group substituted by a C 6-I4 aryl group (an aryl group having 6 to 14 carbon atoms), as defined herein above.
  • arylalkoxy refers to a Ci-] 0 alkoxy group, as defined herein above, substituted by an aryl group, as defined herein above.
  • arylalkoxy groups include benzyloxy and phenethyloxy.
  • aryloxy refers to an oxygen substituted by a C 6 . 14 aryl group, as defined herein above.
  • Examples of aryloxy groups include phenoxy and A- methylphenoxy.
  • arylthio refers to an -S-aryl group, wherein the aryl group is as defined herein above.
  • heteroarylthio refers to an -S-heteroaryl group, wherein the heteroaryl group is as defined herein above.
  • haloalkoxy refers to an alkoxy group which is substituted with 1 to 6 halo groups, wherein the alkoxy group and the halo groups are as defined herein above, and further wherein the halo groups are independently selected.
  • haloaryl refers to an aryl group which is substituted with 1 to 6 halo groups, wherein the aryl group and the halo groups are as defined herein above, and further wherein the halo groups are independently selected.
  • acylamino refers to an -N(R 17 )C( ⁇ O)R 18 group, wherein Rn and Ri 8 are as defined herein above.
  • heteroaryloxy refers to an -O-heteroaryl group, wherein the heteroaryl group is as defined herein above.
  • heteroarylalkoxy refers to a Ci -10 alkoxy group, as defined herein above, substituted by a heteroaryl group, as defined herein above.
  • preventing an increase in a symptom refers to both not allowing a symptom to increase or worsen, as well as reducing the rate of increase in the symptom.
  • a symptom can be measured as the amount of particular disease marker, i.e., a protein.
  • the symptom can be cognitive decline.
  • Preventing an increase means that the amount of symptom ⁇ e.g., protein or cognitive decline) does not increase or that the rate at which it increases is reduced.
  • treating a disease or disorder refers to a slowing of or a reversal of the progress of the disease. Treating a disease or disorder includes treating a symptom and/or reducing the symptoms of the disease.
  • preventing a disease or disorder refers to a slowing of the disease or of the onset of the disease or the symptoms thereof. Preventing a disease or disorder can include stopping the onset of the disease or symptoms thereof.
  • unit dosage form refers to a physically discrete unit, such as a capsule or tablet suitable as a unitary dosage for a human patient.
  • Each unit contains a predetermined quantity of a compound of Formula I, which was discovered or believed to produce the desired pharmacokinetic profile which yields the desired therapeutic effect.
  • the dosage unit is composed of a compound of Formula I in association with at least one pharmaceutically acceptable carrier, salt, excipient, or combination thereof.
  • the patient or subject such as the subject in need of treatment or prevention, may be e.g. a eukaryote, an animal, a vertebrate animal, a mammal, a rodent (e.g. a guinea pig, a hamster, a rat, a mouse), a murine (e.g. a mouse), a canine (e.g. a dog), a feline (e.g. a cat), an equine (e.g. a horse), a primate, a simian (e.g. a monkey or ape), a monkey (e.g. a marmoset, a baboon), an ape (e. g.
  • mice gorilla, chimpanzee, orangutang, gibbon
  • a human The meaning of the terms "eukaryote”, “animal”, “mammal”, etc. is well known in the art and can, for example, be deduced from Wehner und Gehring (1995; Thieme Verlag).
  • animals are to be treated which are economically, agronomically or scientifically important.
  • Scientifically important organisms include, but are not limited to, mice, rats, rabbits, fruit flies like Drosophila melagonaster and nematodes like Caenorhabditis elegans.
  • Non-limiting examples of agronomically important animals are sheep, cattle and pig, while, for example, cats and dogs may be considered as economically important animals.
  • the subject/patient is a mammal; more preferably, the subject/patient is a human.
  • a 40 mg dose of a compound of Formula I refers to, in the case of a twice-daily dosage regimen, a situation where the individual takes 40 mg of a compound of Formula I twice a day, e.g., 40 mg in the morning and 40 mg in the evening.
  • the 40 mg of a compound of Formula I dose can be divided into two or more dosage units, e.g., two 20 mg dosage units of a compound of Formula I in tablet form or two 20 mg dosage units of a compound of Formula I in capsule form.
  • a "pharmaceutically acceptable prodrug” is a compound that may be converted under physiological conditions or by solvolysis to the specified compound or to a pharmaceutically acceptable salt of such compound.
  • a "pharmaceutically active metabolite” is intended to mean a pharmacologically active product produced through metabolism in the body of a specified compound or salt thereof. Metabolites of a compound may be identified using routine techniques known in the art and their activities determined using tests such as those described herein.
  • a "pharmaceutically acceptable salt” is intended to mean a salt that retains the biological effectiveness of the free acids and bases of the specified compound and that is not biologically or otherwise undesirable.
  • a compound for use in the invention may possess a sufficiently acidic, a sufficiently basic, or both functional groups, and accordingly react with any of a number of inorganic or organic bases, and inorganic and organic acids, to form a pharmaceutically acceptable salt.
  • Exemplary pharmaceutically acceptable salts include those salts prepared by reaction of the compounds of the present invention with a mineral or organic acid or an inorganic base, such as salts including sulfates, pyrosulfates, bisulfates, sulfites, bisulfites, phosphates, monohydrophosphates, dihydrophosphates, metaphosphates, pyrophosphates, chlorides, bromides, iodides, acetates, propionates, decanoates, caprylates, acrylates, formates, isobutyrates, caproates, heptanoates, propiolates, oxalates, malonates, succinates, suberates, sebacates, fumarates, maleates, butyne-1 ,4 dioates, hexyne-l,6-dioates, benzoates, chlorobenzoates, methylbenzoates, dinitrobenzoates, hydroxybenzoates, methoxybenzoates
  • a "pharmaceutically acceptable carrier” refers to a non-API (API refers to Active Pharmaceutical Ingredient) substances such as disintegrators, binders, fillers, and lubricants used in formulating pharmaceutical products. They are generally safe for administering to humans according to established governmental standards, including those promulgated by the United States Food and Drug Administration and the European Medical Agency.
  • the compounds of Formula I can contain asymmetric carbon atoms and can therefore exist in racemic and optically active forms.
  • optical isomers or enantiomers, racemates, tautomers, and diastereomers are also encompassed in the compounds of Formula I in all embodiments described herein.
  • the methods of the present invention include the use of all such isomers and mixtures thereof. Methods of separation of enantiomeric and diastereomeric mixtures are well known to one skilled in the art.
  • the present invention encompasses any isolated racemic or optically active form of compounds described in Formula I, or any mixture thereof.
  • the compounds of the invention have a trans configuration around the cyclopropyl ring as in trans-phenylcyclopropylamine.
  • the compounds of the invention have a cis configuration around the cyclopropyl ring as in cis- phenylcyclopropylamine.
  • compounds according to Formula I can be effective at an amount of from about 0.01 ⁇ g/kg to about 100 mg/kg per day based on total body weight.
  • the active ingredient may be administered at once, or may be divided into a number of smaller doses to be administered at predetermined intervals of time.
  • the suitable dosage unit for each administration can be, e.g., from about 1 ⁇ g to about 2000 mg, preferably from about 5 ⁇ g to about 1000 mg. It should be understood that the dosage ranges set forth above are exemplary only and are not intended to limit the scope of this invention.
  • the therapeutically effective amount for each active compound can vary with factors including but not limited to the activity of the compound used, stability of the active compound in the patient's body, the severity of the conditions to be alleviated, the total weight of the patient treated, the route of administration, the ease of absorption, distribution, and excretion of the active compound by the body, the age and sensitivity of the patient to be treated, and the like, as will be apparent to a skilled artisan.
  • the amount of administration can be adjusted as the various factors change over time.
  • the active compounds can be incorporated into a formulation that includes pharmaceutically acceptable carriers such as binders (e.g., gelatin, cellulose, gum tragacanth), excipients (e.g., starch, lactose), lubricants (e.g., magnesium stearate, silicon dioxide), disintegrating agents (e.g., alginate, Primogel, and corn starch), and sweetening or flavoring agents (e.g., glucose, sucrose, saccharin, methyl salicylate, and peppermint).
  • binders e.g., gelatin, cellulose, gum tragacanth
  • excipients e.g., starch, lactose
  • lubricants e.g., magnesium stearate, silicon dioxide
  • disintegrating agents e.g., alginate, Primogel, and corn starch
  • sweetening or flavoring agents e.g., glucose, sucrose, saccharin, methyl salicylate, and peppermint
  • Suitable oral formulations can also be in the form of suspension, syrup, chewing gum, wafer, elixir, and the like. If desired, conventional agents for modifying flavors, tastes, colors, and shapes of the special forms can also be included.
  • the active compounds can be dissolved in an acceptable lipophilic vegetable oil vehicle such as olive oil, corn oil and safflower oil.
  • the active compounds can also be administered parenterally in the form of solution or suspension, or in lyophilized form capable of conversion into a solution or suspension form before use.
  • diluents or pharmaceutically acceptable carriers such as sterile water and physiological saline buffer can be used.
  • Other conventional solvents, pH buffers, stabilizers, anti-bacteria agents, surfactants, and antioxidants can all be included.
  • useful components include sodium chloride, acetates, citrates or phosphates buffers, glycerin, dextrose, fixed oils, methyl parabens, polyethylene glycol, propylene glycol, sodium bisulfate, benzyl alcohol, ascorbic acid, and the like.
  • the parenteral formulations can be stored in any conventional containers such as vials and ampoules.
  • Topical administration examples include nasal, bucal, mucosal, rectal, or vaginal applications.
  • the active compounds can be formulated into lotions, creams, ointments, gels, powders, pastes, sprays, suspensions, drops and aerosols.
  • one or more thickening agents, humectants, and stabilizing agents can be included in the formulations. Examples of such agents include, but are not limited to, polyethylene glycol, sorbitol, xanthan gum, petrolatum, beeswax, or mineral oil, lanolin, squalene, and the like.
  • a special form of topical administration is delivery by a transdermal patch. Methods for preparing transdermal patches are disclosed, e.g., in Brown, et al. (1988) Ann. Rev. Med. 39:221 -229 which is incorporated herein by reference.
  • Subcutaneous implantation for sustained release of the active compounds may also be a suitable route of administration. This entails surgical procedures for implanting an active compound in any suitable formulation into a subcutaneous space, e.g., beneath the anterior abdominal wall. See, e.g., Wilson et al. (1984) J. Clin. Psych. 45:242-247.
  • Hydrogels can be used as a carrier for the sustained release of the active compounds. Hydrogels are generally known in the art. They are typically made by crosslinking high molecular weight biocompatible polymers into a network, which swells in water to form a gel like material. Preferably, hydrogels are biodegradable or biosorbable.
  • hydrogels made of polyethylene glycols, collagen, or poly(glycolic-co-L-lactic acid) may be useful. See, e.g., Phillips et al. (1984) J. Pharmaceut. ScL, 73 : 1718-1720.
  • the active compounds can also be conjugated, to a water soluble non- immunogenic non-peptidic high molecular weight polymer to form a polymer conjugate.
  • an active compound is covalently linked to polyethylene glycol to form a conjugate.
  • a conjugate exhibits improved solubility, stability, and reduced toxicity and immunogenicity.
  • the active compound in the conjugate can have a longer half-life in the body, and exhibit better efficacy. See generally, Burnham (1994) Am. J. Hosp. Pharm. 15 :210-218. PEGylated proteins are currently being used in protein replacement therapies and for other therapeutic uses.
  • PEGylated interferon PEG-INTRON A®
  • PEGylated adenosine deaminase ADAGEN®
  • SCIDS severe combined immunodeficiency disease
  • PEGylated L-asparaginase ONCAPSPAR®
  • ALL acute lymphoblastic leukemia
  • Controlled release of an active compound can also be achieved by incorporating the active ingredient into microcapsules, nanocapsules, or hydrogels generally known in the art.
  • Other pharmaceutically acceptable prodrugs of the compounds of this invention include, but are not limited to, esters, carbonates, thiocarbonates, N-acyl derivatives, N- acyloxyalkyl derivatives, quaternary derivatives of tertiary amines, N-Mannich bases, Schiff bases, aminoacid conjugates, phosphate esters, metal salts and sulfonate esters.
  • Liposomes can also be used as carriers for the active compounds of the present invention.
  • Liposomes are micelles made of various lipids such as cholesterol, phospholipids, fatty acids, and derivatives thereof. Various modified lipids can also be used. Liposomes can reduce the toxicity of the active compounds, and increase their stability. Methods for preparing liposomal suspensions containing active ingredients therein are generally known in the art. See, e.g., U.S. Patent No. 4,522,81 1 ; Prescott, Ed., Methods in Cell Biology, Volume XIV, Academic Press, New York, N. Y. (1976).
  • the active compounds can also be administered in combination with another active agent that synergistically treats or prevents the same symptoms or is effective for another disease or symptom in the patient treated so long as the other active agent does not interfere with or adversely affect the effects of the active compounds of this invention.
  • additional active agents include but are not limited to anti-inflammation agents, antiviral agents, antibiotics, antifungal agents, antithrombotic agents, cardiovascular drugs, cholesterol lowering agents, anti-cancer drugs, hypertension drugs, and the like.
  • antineoplastic agents that can be used in combination with the compounds and methods of the present invention include, in general, and as appropriate, alkylating agents, anti-metabolites, epidophyllotoxins, antineoplastic enzymes, topoisomerase inhibitors, procarbazines, mitoxantrones, platinum coordination complexes, biological response modifiers and growth inhibitors, hormonal/anti-hormonal therapeutic agents and haematopoietic growth factors.
  • Exemplary classes of antineoplastic include the anthracyclines, vinca drugs, mitomycins, bleomycins, cytotoxic nucleosides, epotbilones, discodermolides, pteridines, diynenes and podophyllotoxins.
  • Particularly useful members of those classes include, for example, carminomycin, daunorubicin, aminopterin, methotrexate, methopterin, dichloromethotrexate, mitomycin C, porfiromycin, 5- fluorouracil, 6-mercaptopurine, gemcitabine, cytosine arabinoside, podophyllotoxin or podo-phyllotoxin derivatives such as etoposide, etoposide phosphate or teniposide, melphalan, vinblastine, vincristine, leurosidine, vindesine, leurosine, paclitaxel and the like.
  • antineoplastic agents include estramustine, carboplatin, cyclophosphamide, bleomycin, gemcitibine, ifosamide, melphalan, hexamethyl melamine, thiotepa, cytarabin, idatrexate, trimetrexate, dacarbazine, L- asparaginase, camptothecin, CPT-I l , topotecan, ara-C, bicalutamide, flutamide, leuprolide, pyridobenzoindole derivatives, interferons and interleukins.
  • Rl , R2, R3, R4, R5, R6 and R7 represent a hydrogen atom and R8 represents an optionally substituted aryl, heteroaryl, or heterocyclic group
  • R8 represents an optionally substituted aryl, heteroaryl, or heterocyclic group
  • trans-Phenylcyclopropylamine (ALDRICH) of Formula (II) is reductively akyiated using commercially available aldehydes of Formula (III) R8CHO, in which R8 is as defined earlier (e.g., in the markush groups described above), to give the compounds of Formula (IV), which are a particular case of the compounds claimed in the present invention.
  • Rl , R2, R3, R4, R5 and R6 represent a hydrogen atom
  • R7 represents alkyl or cycloalkyl
  • R8 represents an optionally substituted aryl, heteroaryl, or heterocyclic group
  • trans-phenylcyclopropylamine (ALDRICH) of Formula (II) is reductively alkylated using commercially available ketones of Formula (X), in which R7 and R8 are as defined earlier, to give the compounds of Formula (XI), which are also subjects of the present invention.
  • trans phenylcyclopropylammo derivatives of formula (XIV) (including trans ((1S, 2R), (IR, 2S)) version as well the individual diastereoisomers corresponding to (IS, 2R) and (IR, 2S) can be used) are reacted with methyl 2-bromoacetate in acetonit ⁇ le usmg N,N-dnsopropylethylamme as a base to give methyl 2-((trans)-2- phenylcyclopropylamino)acetate derivatives of formula (XVI) Hydrolysis with lithium hydroxide using tetrahydrofurane-water solution as a solvent and later reaction with t-butyl dicarbonate in tetrahydrofurane leads to 2-(tert- butoxycarbonyl((trans)-2-phenylcyclopropy])amino)acetic acid derivatives of formula (XVII), which are reacted with commercially available formamidoxime derivatives of formula
  • Trimethylsulfoxonium iodide (0.62 g, 2.82 mmol) was added in portions to a solution of r-BuOK (0.32 g, 2.82 mmol) in dry DMSO (5 mL). After 10 min a solution of l -(benzyloxy)-4-[(E)-2-nitrovinyl]benzene (0.60 g, 2.35 mmol) in DMSO (5 mL) was transferred via canula and the mixture was stirred at room temperature for 6 h. The reaction was poured over water (10 mL) and extracted with Et 2 O (3x10 mL); the organic layers were washed with brine (2x15 mL), dried over anhydrous Na 2 SO 4 and filtered.
  • the compounds of the invention can be tested for their ability to inhibit LSD l .
  • the ability of the compounds of the invention to inhibit LSDl can be tested as follows. Human recombinant LSDl protein was purchased from BPS Bioscience Inc. In order to monitor LSDl enzymatic activity and/or its inhibition rate by our inhibitor(s) of interest, di-methylated H3-K4 peptide (Millipore) was chosen as a substrate. The demethylase activity was estimated, under aerobic conditions, by measuring the release of H 2 O 2 produced during the catalytic process, using the Amplex® Red p ⁇ roxide/peroxidase-coupled assay kit (Invitrogen).
  • Amplex® Red reagent and horseradish peroxidase (HPR) solution were added to the reaction according to the recommendations provided by the supplier (Invitrogen), and left to incubate for 30 extra minutes at room temperature in the dark.
  • a 1 ⁇ M H 2 O 2 solution was used as a control of the kit efficiency.
  • the maximum demethylase activity of LSDl was obtained in the absence of inhibitor and corrected for background fluorescence in the absence of LSDl .
  • the Ki of each inhibitor was estimated at half of the maximum activity.
  • a number of the compounds of the invention were tested for their ability to inhibit LSDl and were found to have Ki values lower than 100 ⁇ M, including many of the compounds in examples that were tested.
  • Compound of examples 3 and 17, were found to have Ki values for LSDl of less than 10 ⁇ M.
  • Compounds of examples 1 , 8, 9, 11 were found to have Ki values for LSDl of less than 1 micromolar.
  • Parnate (2- trans phenylcyclopropylamine) was found to have a Ki of from about 15 to 35 micromolar depending on the enzyme preparation.
  • Egr-1 is a tumor suppressor gene in many contexts (see e.g., Calogero et al. (2004) Cancer Cell International 4: 1 exogenous expression of EGR-I resulted in growth arrest and eventual cell death in primary cancer cell lines; Lucerna et al. (2006) Cancer Research 66, 6708-6713 show that sustained expression of Egr-1 causes antiangiogeneic effects and inhibits tumor growth in some models; Ferraro et al. ((2005) J. Clin. Oncol.
  • LSD l is a therapeutic target for cancer.
  • the instant inventors have discovered a class of LSD l inhibitors that can be used to treat diseases where LSDl is implicated as a therpautic target like cancer. According, the phenylcyclopropylamine compounds of the invention can be used to treat and/or prevent such diseases.
  • Example 81 Biological Assays - Monoamine Oxidase Assays
  • MAO-A and MAO-B Human recombinant monoamine oxidase proteins MAO-A and MAO-B were purchased from Sigma Aldrich. MAOs catalyze the oxidative deamination of 1°, 2° and 3° amines. In order to monitor MAO enzymatic activities and/or their inhibition rate by inhibitor(s) of interest, a fluorescent-based (inhibitor)-screening assay was set up. 3-(2-Aminophenyl)-3- oxopropamamine (kynuramine dihydrobromide, Sigma Aldrich), a non fluorescent compound was chosen as a substrate. Kynuramine is a non-specific substrate for both MAOs activities. While undergoing oxidative deamination by MAO activities, kynuramine is converted into 4-hydroxyquinoline (4-HQ), a resulting fluorescent product.
  • 3-(2-Aminophenyl)-3- oxopropamamine kynuramine dihydrobromide, Sigma Aldrich
  • the monoamine oxidase activity was estimated by measuring the conversion of kynuramine into 4-hydroxyquinoline. Assays were conducted in 96-well black plates with clear bottom (Corning) in a final volume of 100 ⁇ L. The assay buffer was 100 mM HEPES, pH 7.5. Each experiment was performed in triplicate within the same experiment.
  • the maximum of oxidative deamination activity was obtained by measuring the amount of 4- hydroxyquinoline formed from kynuramine deamination in the absence of inhibitor and corrected for background fluorescence in the absence of MAO enzymes.
  • the Ki of each inhibitor was measure at Vmax/2.
  • a number of the compounds of the invention were tested for their ability to inhibit MAO-A using the above described assay and were found to have Ki values for MAO-A higher than that for MAO-B (i.e., inhibit MAO-B better than MAO-A) e.g., like the compounds of Examples 11 and 13 which both a better MAO-B inhbititors than MAO-A inhbitors which MAO-B IC50 values below 50 micromolar.
  • LSDl selective inhibitors have IC50 values for LSDl which are at least 2-fold lower than the IC50 value for MAO-A and/or MAO-B.
  • IC50 values for LSDl which are at least 2-fold lower than the IC50 value for MAO-A and/or MAO-B.
  • Example 2 which has an IC50 for LSD l which is about 10-fold lower than for MAO-A and MAO-B.
  • Example 16 which has an IC50 for LSDl which is more than 5-fold lower than the IC50 for MAO-A and MAO-B.
  • Example 17 which has an IC50 which is more than 3-fold lower for LSDl than MAO-A and MAO-B.
  • the invention also provides dual inhibitors selective for LSDl and MAO-B.
  • Dual LSD1/MAO-B selective inhibitors have IC50 values for LSDl and MAO-B which are at least 2-fold lower than the IC50 value for MAO-A.
  • One example of a dual LSD1/MA0-B selective inhibitor is given in Example 11 which has an IC50 for LSD l and MAO-B which is about 2-fold lower than for MAO-A.
  • Another example of a dual LSDl /MAO-B inhibitor is iven in Example 7 where the MAO-B IC50 is less than half the value it is for MAO-A and the LSDl IC50 is about 1 micromolar. Table 1
  • the compounds of Formula I can have IC50 values for LSDl of less than that for parnate, less than 10 micromolar, less than 1 micromolar, and less than 500 nanomolar. Typically the IC50 values of the compounds of Formula I for LSDl are less than 1 micromolar.
  • the compounds of Formula I can have IC50 values for MAO-A of less than 20 micromolar, less than 10 micromolar, and less than 5 micromolar. Typically the IC50 values of the compounds of Formula I for MAO-A are greater than 1 micromolar.
  • the compounds of Formula I can have IC50 values for MAO-B of less than 20 micromolar, less than 10 micromolar, and less than 5 micromolar. Typically the IC50 values of the compounds of Formula I for MAO-B are greater than 1 micromolar. Most of the compounds of the Examples are selective LSDl inhibitors in that they inhibit LSD l to a greater extent than MAO-A and MAO-B. Some of the compounds of the Examples inhibit both MAO-B and LSDl to a greater extent than MAO-A.
  • the compounds disclosed herein are surprisingly and significantly more potent than tranylcypromine for LSD l inhibition.
  • Han et al. Euro. J. Pharma. (2008) doi: 10.1016/j.ejphar.2008.12.025
  • the compounds of Formula I may be used as a neuroprotectant (e.g., used to treat and/or prevent conditions characterized by neurodegeneration).
  • the compounds of Formula I are potent LSDl inhibitor they can be used to treat and/or prevent diseases where LSDl inhibition is desirable, e.g., cancer.

Abstract

The present invention relates to phenylcyclopropylamine derivatives. In particular, pharmaceutical compositions comprising phenylcyclopropylamine derivatives are provided. The compounds of this invention can, inter alia, be used for the treatment and the prevention of cancer as well as neurodegenerative diseases or disorders.

Description

PHENYLCYCLOPROPYLAMINE DERIVATIVES AND THEIR MEDICAL USE
The present invention relates to phenylcyclopropylamine derivatives. In particular, pharmaceutical compositions comprising phenylcyclopropylamine derivatives are provided. The compounds of this invention can, inter alia, be used for the treatment and the prevention of cancer as well as neurodegenerative diseases or disorders.
Cancer is prevalent: there were about 3.2 million cancer cases diagnosed (53% men, 47% women) and 1.7 million deaths from cancer (56% men, 44% women) in Europe (Ferlay et al. (2007) Ann. Oncol. 18(3):581-92). In the United States, the probability of developing invasive cancer is 38% for females and 46% for males that live to be 70 years older and older. In the US about 1.4 million new cases of cancer are expected for 2006. Although the five year survival rate for cancer is now 65%, up from about 50% in the mid-nineteen seventies, cancer is deadly. It is estimated that 565,000 people in the United States will die from cancer in 2006 (American Cancer Society, Surveillance Research, 2006). Despite tremendous advances in cancer treatment and diagnosis, cancer remains a major public health concern. Accordingly, there is a need for new therapeutics with activity in cancer.
Another health crisis is facing industrialized nations. As the population in these countries age, neurodegenerative diseases are affecting more and more people, posing a tremendous economic burden to national health systems. Alzheimer's disease is the largest neurodegenerative disease; disease modifying drugs have long been sought, but to-date, none have been identified. Other neurodegenerative conditions include Parkinson's disease, Huntington's disease, Lewy Body dementia, and which are all characterized by disease progression which robs the patients of their ability to perform normal daily activities, eventually leading to death. One similar characteristic amongst many cancers and neurodegenerative diseases is aberrant gene expression. A number of compounds have been shown to alter gene expression, including histone deacetylase inhibitors which alter the histone acetylation profile of chromatin. Histone deacetylase inhibitors have been shown to alter gene expression. Another modification that is involved in regulating gene expression is lysine methylation. Methylation of histone lysines has recently been shown to be important in regulating gene expression. A group of enzymes known as lysine demethylases are involved in this histone modification. One particular human histone lysine demethylase enzyme called Lysine Specific Demethylase-1 (LSDl) was recently discovered (Shi et al. (2004) Cell 1 19:941). LSDl is also involved in regulating the methylation of some non-histone lysines. LSDl has a fair degree of structural similarity, and amino acid identity/homology to monoamine oxidases, all of which (i.e., MAO-A, MAO-B and LSDl) are flavin dependent oxidases. Recent experiments with LSDl have shown that it is involved in diverse process such as carcinogenesis (Kahl et al. (2006) Cancer Res. 66: 1341-1 1347) and vascular inflammation (Reddy et al. (2008) Circ. Res. 103 :615). It was found that a commercially available antidepressant, Parnate®, which targets monoamine oxidase (MAO), also inhibits LSDl at clinically relevant concentrations (Lee et al. (2006) Chem. Biol. 13 :563-567). Lee et al. initially reported that Parnate was a better inhibitor of LSDl than either MAO-A and MAO-B but subsequent study by some of the same authors found "IC50 values for 2-PCPA of 20.7 ± 2.1 μM for LSDl , 2.3 ± 0.2 μM for MAO A, and 0.95 ± 0.07 μM for MAO B." See Schmidt et al. (2007) Biochemistry 46(14)4408-4416. Thus, Parnate (2-PCPA; tranylcypromine) is a better inhibitor of MAO-A and MAO-B as compared to LSDl . Monoamine oxidase inhibitors are useful for treating a number of conditions including depression and neurodegenerative conditions like Parkinson's disease. Parnate is part of a class of compounds known as phenylcyclopropylamines which are related to another group of clinical useful MAO inhibitors called propargylamines, exemplified by Pargyline which also inhibits LSDl . Additionally, derivatives of Parnate also can inhibit LSDl (Gooden et al. (2008) Bioorg. Med. Chem. Let. 18:3047-3051). Another class of compounds was recently disclosed to inhibit LSDl activity: polyamines (Huang et al. (2007) PNAS 104:8023-8028). The polyamines inhibit LSDl modestly and were shown to cause the reexpression of genes aberrantly silenced in cancer cells. Lee et al. ((2006) Chem. Biol. 13 :563-567) reported that tranylcypromine inhibits histone H3K4 demethylation and can derepress Egrl gene expression in some cancer lines. A body of evidence is accumatling that Egr-1 is a tumor suppressor gene in many contexts. Calogero et al((2004) Cancer Cell International 4: 1) reported that Egr-1 is downregulated in brain cancers and exogenous expression of Egr-1 resulted in growth arrest and eventual cell death in primary cancer cell lines. Lucerna et al. ((2006) Cancer Research 66, 6708-6713) showed that sustained expression of Egr-1 causes antiangiogeneic effects and inhibits tumor growth in some models. Ferraro et al ((2005) J Clin Oncol. Mar 20;23(9): 1921-6) reported that Egr-1 is downregulated in lung cancer patients with a higher risk of recurrence and may be more resistant to therapy. Scoumanne et al. ((2007) J Biol Chem. May 25;282(21): 15471-5) observed that LSDl is required for cell proliferation. They found that deficiency in LSDl leads to a partial cell cycle arrest in G2/M and sensitizes cells to growth suppression induced by DNA damage. Kahl et al ((2006) Cancer Res. 66(23): 11341-7) found that LSDl expression is correlated with prostate cancer aggressiveness. Metzger et al. ((2005) Nature 15;437(7057):436-9) reported that LSDl modulation by siRNA and pargyline regulates androgen receptor (AR) and may have therapeutic potential in cancers where AR plays a role, like prostate, testis, and brain cancers. Thus, a body of evidence has implicated LSDl in a number of cancers, which suggestes that LSD l is a therapeutic target for cancer.
The phenylcyclopropylamines have been the subject of many studies designed to elucidate an SAR for MAO inhibition. Kaiser et al. ((1962) J. Med. Chem. 5: 1243- 1265) and Zirkle et al. ((1962) J. Med. Chem. 1265-1284) have disclosed the synthesis and activity of a number of phenylcyclopropylamine related compounds. Zirkle et al. ((1962) J. Med. Chem. 1265-1284) reported that mono- and disubstitution of the amino group of trans-2-phenylcyclopropylamine with methyl decreases the activity only slightly whereas monosubstitution with larger groups like alkyl and aralkyl groups results in considerable loss of activity in the tryptamine potentiation assay for MAO activity. Studies have also been conducted with phenylcyclopropylamine related compounds to determine selectivity for MAO-A versus MAO-B since MAO-A inhibitors can cause dangerous side-effects (see e.g., Yoshida et al. (2004) Bioorg. Med Chem. 12(10):2645-2652; Hruschka et al. (2008) Biorg Med Chem. (16):7148-7166; Folks et al. (1983) J. Clin. Psychopharmacol. (3)249; and Youdim et al. (1983) Mod. Probl. Pharmacopsychiatry (19):63). Other phenylcyclopropylamine type compounds are disclosed in Bolesov et al ((1974) Zhurnal Organicheskoi Khimii 10:8 1661-1669) and Russian Patent No. 230169 (19681030). Gooden et al. ((2008) Bioorg. Med. Chem. Let. 18:3047-3051) describe the synthesis of phenylcyclopropylamine derivatives and analogs as well as their activity against MAO-A, MAO-B, and LSDl . None of the compounds made in Gooden et al. showed a lower Ki for LSDl as compared to either MAO-A or MAO- B. Additionally, most of the Gooden et al. phenylcyclopropylamine derivatives were better inhibitors of MAO-A as compared to MAO-B. Recently, Han et al. (Euro. J. Pharma. (2008) doi: 10.1016/j.ejphar.2008.12.025) reported that phenylcyclopropylamine displays neuroprotective activity in PC 12 cells.
Phenylcyclopropylamine derivatives are also disclosed in US-A-3, 106,578, US-A-6,21 1 ,244, US-A-2003/236225, WO 03/093297, WO 2007/025144, and in Westland RD, et al. J Med Chem. 1968. 11 (4): 824-829.
In view of the lack of adequate treatments for conditions such as cancer and neurodegeneration, there is a desperate need for disease modifying drugs and drugs that work by inhibiting novel targets. There is a need for the development of LSDl selective inhibitors particularly those which selectively inhibit LSDl and those which are dual inhibitors of MAO-B/LSD1.
This problem is solved by the embodiments of the present invention as characterized herein below, in the appended examples and the claims.
The present invention relates to phenylcyclopropylamine derivatives. In particular, pharmaceutical compositions comprising phenylcyclopropylamine derivatives are provided. The compounds of this invention can, inter alia, be used for the treatment and the prevention of diseases. The present invention provides compounds of Formula I, pharmaceutical compositions comprising a compound of Formula I and a pharmaceutically acceptable carrier, and their use for treating and/or preventing diseases. One use of the compounds of Formula I is for treating cancer. Another use for the compounds of Formula I is to inhibit LSDl . Another use of the compounds of Formula I is as dual inhibitors of MAO-B and LSDl . Compounds of Formula I can have monoamine oxidase inhibition activity and therefore can be used to treat and/or prevent disease like depression and Parkinson's disease as well as other neurodegenerative conditions.
In one embodiment, the invention provides a pharmaceutical composition comprising a compound of Formula I and a pharmaceutically acceptable carrier:
Figure imgf000006_0001
FORMULA I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyϊ, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido;
R6 is chosen from -H and alkyl;
R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CHi)nNH(CH2)H-, - (CH2)nO(CH2)n-, and -(CHi)nS(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof.
In one specific aspect of the pharmaceutical compositions of this embodiment, R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect, R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect, R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CHi)n-(CHi)n-, - (CHi)nNH(CHi)n-, -(CHi)nO(CHi)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CHa)n-(CH2)H- and -(CHi)nO(CHa)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one specific aspect of the pharmaceutical compositions of this embodiment, R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In a more specific aspect, R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect, R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CH2)n-(CH2)n-, - (CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CHi)nO(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
The invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I which is a selective inhibitor of LSDl . LSD l selective inhibitors have IC50 values for LSDl which are at least 2- fold lower than the IC50 value for MAO-A and/or MAO-B.
The invention also provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I which is a dual inhibitor selective for LSDl and MAO-B. Dual LSD1/MAO-B selective inhibitors have IC50 values for LSDl and MAO-B which are at least 2-fold lower than the IC50 value for MAO-A.
In one embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer:
Figure imgf000009_0001
FORMULA I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, - (CH2)nO(CH2)n-, and -(CH2)JiS(CH2)Ii-, where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof.
In one specific aspect of this embodiment, the cancer is prostate cancer. In another specific aspect of this embodiment, the cancer is brain cancer. In yet another specific aspect of this embodiment, the cancer is breast cancer. In yet another specific aspect of this embodiment, the cancer is lung cancer. In yet another specific aspect of this embodiment, the cancer is testicular cancer. In yet another specific aspect of this embodiment, the cancer is colorectal cancer. In yet another specific aspect of this embodiment, the cancer is blood cancer (e.g., leukemia). In yet another specific aspect of this embodiment, the cancer is skin cancer.
In one specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfmyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CHi)nS(CH2)Ii-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and - (CHi)nO(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from - (CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one embodiment, the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder:
Figure imgf000013_0001
FORMULA I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido;
R6 is chosen from -H and alkyl;
R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-,
-(CH2)nO(CH2)n-, and -(CHynS(CHi)n-, where each n is independently chosen from
0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof.
In one specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder where R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfϊnyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder where R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CHa)n-(CHa)n-, - (CH2)nNH(CH2)n-, -(CH2)J1O(CH2),,-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CHa)n-(CHi)n- and -(CHa)nO(CHa)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond. In one specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder where R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder where R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease or disorder where R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CHi)n-(CHi)n-, - (CHi)nNH(CHi)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one embodiment, the invention provides a compound of Formula I:
Figure imgf000016_0001
wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is a -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, - (CHi)nO(CH2)Ii-, and -(CHa)nS(CHa)n-, and where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof; with the provision that when Rl , R2, R3, R4, R5, R6, and R7 are all hydro and L is a bond, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl, A- methoxyphenyl, 4-chlorophenyl, or furan-2-yl, and also when Rl , R2, R3, R4, R5, and R7 are all hydro, L is a bond and R8 is phenyl, then R6 is not methyl, ethyl or isopentyl.
In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
In one specific aspect of this embodiment the invention provides a compound of Formula I where R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is -L- hεtεroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CH2)I1-(CH2)I1-, - (CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)M-(CH2),,- and -(CHi)nO(CH2)I1-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one specific aspect of this embodiment the invention provides a compound of Formula I where R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. According to the aspects described in this paragraph each L is independently chosen from -(CHa)n-(CHi)n-, -(CH2)nNH(CH2)n-, - (CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from - (CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or - CH2-. In a more specific definition, L is a covalent bond.
In one embodiment, the invention provides a compound of Formula I wherein R8 is heterocyclyl having from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heteroaryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido; with the provision that when Rl , R2, R3, R4, R5, R6, and R7 are all hydro, then R8 is not furan-2-yl.
In one embodiment, the invention provides compounds of Formula I wherein R6 and R7 are hydro.
In one embodiment, the invention provides a compound of Formula I wherein R1 -R7 are each hydro and R8 is aryl group having from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, - L-aryl, -L-heteroaryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; with the provision that when Rl , R2, R3, R4, R5, R6, and R7 are all hydro, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4- bromophenyl, 4-methoxyphenyl, or 4-chlorophenyl.
In one embodiment, the invention provides a compound of Formula I wherein the phenyl ring attached to the cyclopropyl ring has at least one substituent that is not hydro, i.e. at least one of Rl to R5 is not hydro.
In one embodiment, the invention provides a method of treating a cancer comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier. Accordingly, in one embodiment, the invention provides a method for treating or preventing a cancer comprising the administration of a therapeutically effective amount of a compound of Formula I or a pharmaceutical composition comprising said compound and a pharmaceutically acceptable carrier to a subject (e.g. a human) in need of such a treatment or prevention. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer. In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for treating and/or preventing cancer. In a specific aspect of the embodiments of this paragraph, the cancer is chosen from breast cancer, lung cancer, prostate cancer, testicular cancer, brain cancer, colorectal cancer, blood cancer (e.g., leukemia), or skin cancer. In a more specific aspect of the embodiments of this paragraph, the cancer is chosen from breast cancer, lung cancer, prostate cancer, testicular cancer, or brain cancer.
In one embodiment, the invention provides a method of inhibiting LSD l activity comprising administering, to a patient in need of treatment, an amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier sufficient to inhibit LSDl activity. In a related embodiment, the invention provides a compound of Formula I for inhibiting LSDl . In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for inhibiting LSDl .
In one embodiment, the invention provides a method of treating a neurodegenerative disease or disorder comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier. Accordingly, in one embodiment, the invention provides a method for treating or preventing a neurodegenerative disease comprising the administration of a therapeutically effective amount of a compound of Formula I or a pharmaceutical composition comprising said compound and a pharmaceutically acceptable carrier to a subject (e.g. a human) in need of such a treatment or prevention. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease. In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for treating and/or preventing a neurodegenerative disease. In a specific aspect of the embodiments of this paragraph, the neurodegenerative disease or disorder is chosen from Alzheimer's disease, Parkinson's disease, Huntington's disease, or Lewy Body dementia.
In one embodiment, the invention provides a method of inhibiting monoamine oxidase activity comprising administering, to a patient in need of treatment, an amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier sufficient to inhibit monoamine oxidase activity. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing Parkinson's disease and/or depression. In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for inhibiting monoamine oxidase. In one specific aspect of this embodiment, the monoamine oxidase is MAO-B. In one embodiment, the invention provides a method for identifying LSDl selective inhibitors comprising determining the ability of a test compound to inhibit LSDl and monoamine oxidase (MAO-A and/or MAO-B) wherein a test compound that inhibits LSD l better than monoamine oxidase (MAO-A and/or MAO-B) is LSD l selective. In one aspect of this embodiment, the test compound is chosen from a phenylcyclopropylamine derivative, homolog or analog.
In one embodiment, the invention provides a method for identifying a dual inhibitor selective for LSDl /MAO-B as compared to MAO-A comprising determining the ability of a test compound to inhibit LSDl and monoamine oxidase (MAO-A and MAO-B) wherein a test compound that inhibits LSDl and MAO-B better than LSDl and monoamine oxidase (MAO-A and/or MAO-B) is a dual LSDl /MAO-B selective inhibitor. In one aspect of this embodiment, the test compound is chosen from a phenylcyclopropylamine derivative, homolog or analog.
Phenylcyclopropylamine derivatives, homologs, and analogs can be made by methods known in the art e.g., including, but not limited to the methods disclosed herein and in the references cited herein.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.
Other features and advantages of the invention will be apparent from the following detailed description, and from the claims.
The present invention relates to compounds, the identification of compounds and their use for treating and/or preventing diseases. The present invention provides compounds of Formula I, pharmaceutical compositions comprising a compound of Formula I and a pharmaceutically acceptable carrier, and their use for treating and/or preventing diseases. One use of the compounds of Formula I is to treat and/or prevent cancer. Compounds of the invention also inhibit monoamine oxidases, and can therefore be used for treating and/or preventing a disease in which monoamine oxidase inhibition is useful. Some compounds of Formula I can be used as LSDl selective inhibitors that inhibit LSDl to a greater extent than MAO-A and/or MAO- B. Some compounds of Formula I can be used as dual LSD1/MAO-B selective inhibitors that inhibit LSD l and MAO-B to a greater extent than MAO-A. Some of the compounds of Formula I can be used as inhibitors of MAO-A, MAO-B and LSDl . In particular it was surprisingly found that phenylcyclopropylamine derivatives with monosubstitution on the amine group with ring bearing substituents yields compounds with unexpectedly potent LSDl inhibition. For example, some of the compounds of Formula I have IC50 values for LSDl inhibition of less than 1 micromolar (see Table 1 ) which makes them at least 20-30-fold more potent than tranylcypromine for LSDl inhibition. Furthermore, these types of compounds also are potent inhibitors of the monoamine oxidases.
In one embodiment, the invention provides a pharmaceutical composition comprising a compound of Formula I and a pharmaceutically acceptable carrier:
Figure imgf000023_0001
FORMULA I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfϊnyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido;
R6 is chosen from -H and alkyl;
R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CHa)nNH(CHi)n-, -
(CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, where each n is independently chosen from 0,
1 , 2, and 3 ; and pharmaceutically acceptable salts thereof.
In one specific aspect of the pharmaceutical compositions of this embodiment, R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect, R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect, R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CHa)n-(CH2),!-, - (CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one specific aspect of the pharmaceutical compositions of this embodiment, R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryioxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In a more specific aspect, R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect, R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CH2)H-(CHa)n-, - (CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one aspect of this embodiment, the 0-3 substituents on the ring or ring system of R8 are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1-3 alkyl)2, -NH(Cj-3 alkyl), -C(=O)NH2, -C(=O)NH(Ci-3 alkyl), - C(=O)N(Ci.3 alkyl)2, -S(=O)2(C,-3alkyl), -S(=O)2NH2, -S(O)2N(C1 -3 alkyl)2, - S(=O)2NH(Ci-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In a more specific aspect, the R8 ring or ring system has 1 substituent chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, - NH(Ci-3 alkyl), -C(=0)NH2, -C(=O)NH(Ci-3 alkyl), - C(=O)N(Ci-3 alkyl)2, - S(=O)2(C1-3alkyl), -S(=O)2NH2, -S(O)2N(C1 -3 alkyl)2, - S(=O)2NH(C1-3 alkyl), - CHF2, -OCF3, -OCHF2 -CF3, -CN, -NH2, and -NO2. In an even more specific aspect, the R8 ring or ring system has 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 -3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2V (CH2V, -(CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(Cj-3 alkyl), -C(=O)NH2, - C(^O)NH(C1 -3 alkyl), -C(O)N(Ci-3 alkyl)2, -S(=O)2(Ci-3alkyl), -S(=O)2NH2, - S(O)2N(C1 -3 alkyl)2, -S(=O)2NH(C] -3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, L is independently chosen from -(CH2V(CH2),,-, -(CH2)nNH(CH2V, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2V(CH2V and -(CH2)nO(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
The invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I, as defined herein above, which is a selective inhibitor of LSD l . LSDl selective inhibitors have IC50 values for LSDl which are at least 2-fold lower than the IC50 value for MAO-A and/or MAO-B.
The invention also provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I, as defined herein above, which is a dual inhibitor selective for LSD l and MAO-B. Dual LSD l /MAO- B selective inhibitors have IC50 values for LSD l and MAO-B which are at least 2- fold lower than the IC50 value for MAO-A.
In one embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer:
Figure imgf000028_0001
FORMULA I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido;
R6 is chosen from -H and alkyl;
R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CHi)n-(CHi)n-, -(CHi)nNH(CHi)n-, - (CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof.
In one aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer, wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Cj-3 alkyl)2, -NH(C1-3 alkyl), -C(=O)NH2, - C(=O)NH(Ci-3 alkyl), - C(K))N(C1 -3 alkyl)2, -S(=O)2(C1-3alkyl), -S(=O)2NH2, - S(O)2N(C1 -3 alkyl)2, - S(=O)2NH(Ci.3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In a more specific aspect, the R8 ring or ring system has 1 substituent chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(C1-3 alkyl), -C(=O)NH2, -C(O)NH(C1-3 alkyl), - C(K))N(C1-3 alkyl)2, -S(=O)2(C,.3alkyl), -S(=O)2NH2, -S(O)2N(C1 -3 alkyl)2, - S(=O)2NH(C,-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In an even more specific aspect, the R8 ring or ring system has from 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, aryl alkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2),,- (CH2V, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1-3 alkyl)2, -NH(Cj-3 alkyl), -C(=O)NH2, - C(=O)NH(Ci.3 alkyl), -C(K))N(Ci-3 alkyl)2,
Figure imgf000030_0001
-S(=O)2NH2, - S(O)2N(C1 -3 alkyl)2, -S(=O)2NH(Ci-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-5 -(CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one specific aspect of this embodiment, the cancer is prostate cancer. In another specific aspect of this embodiment, the cancer is brain cancer. In yet another specific aspect of this embodiment, the cancer is breast cancer. In yet another specific aspect of this embodiment, the cancer is lung cancer. In yet another specific aspect of this embodiment, the cancer is testicular cancer. In yet another specific aspect of this embodiment, the cancer is colorectal cancer. In yet another specific aspect of this embodiment, the cancer is blood cancer (e.g., leukemia). In yet another specific aspect of this embodiment, the cancer is skin cancer. In one specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is -L-heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CFb)nS(CLb)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and - (CFb)nO(CFb)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one aspect of this embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer, wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(Ci-3 alkyl), -C(=O)NH2, - C(=O)NH(Ci-3 alkyl), - C(O)N(C1 -3 alkyl)2, -S(=O)2(Ci-3alkyl), -S(=O)2NH2, - S(O)2N(C1 -3 alkyl)2, - S(=O)2NH(d-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In a more specific aspect, the R8 ring or ring system has 1 substituent chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(CL3 alkyl)2, -NH(C1 -3 alkyl), -C(=O)NH2, -C(O)NH(CL3 alkyl), - C(O)N(CL3 alkyl)2, -S(O)2(C1-3alkyl), -S(=O)2NH2, -S(O)2N(C1-3 alkyl)2, - S(O)2NH(Ci-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In an even more specific aspect, the R8 ring or ring system has from 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2),,- (CH2)H-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1 -3 alkyl)2, -NH(C1 -3 alkyl), -C(=O)NH2, - C(=O)NH(Ci.3 alkyl), -C(=O)N(C1-3 alkyl)2, -S(=O)2(C1-3alkyl), -S(=O)2NH2, - S(O)2N(C1-3 alkyl)2, -S(=O)2NH(C] -3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)n0(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer, wherein R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfϊnyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. According to the aspects described in this paragraph each L is independently chosen from -(CHi)n-(CH2),!-, - (CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)n0(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer, wherein R8 is -L-aryl wherein said aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is aryl (L is a bond) wherein said aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I for treating and/or preventing cancer where R8 is a phenyl group wherein said phenyl has from 1-3 (i.e. 1, 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido. According to the aspects described in this paragraph each L is independently chosen from -(CH2)I1-(CHi)n-, - (CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CHi)nO(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CHi-. In a more specific definition, L is a covalent bond.
In one aspect of this embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer, wherein the 0-3 substituents or the 1-3 substituents on the R8 ring or ring system are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(Ci-3 alkyl), -C(=O)NH2, -C(=O)NH(d-3 alkyl), - C(K))N(Ci-3 alkyl)2, -S(=O)2(Ci. 3alkyl), -S(=O)2NH2, -S(O)2N(C1-3 alkyl)2, - S(=O)2NH(Ci-3 alkyl), -CHF2, -OCF3, - OCHF2, -CF3, -CN, -NH2, and -NO2. In a more specific aspect, the R8 ring or ring system has 1 substituent chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(Cj-3 alkyl), -C(=O)NH2, -C(=0)NH(d_3 alkyl), - C(O)N(C1-3 alkyl)2, -S(=O)2(d.3alkyl), -S(=O)2NH2, -S(O)2N(Ci-3 alkyl)2, - S(=O)2NH(Ci-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In an even more specific aspect, the R8 ring or ring system has from 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2)n- (CH2),,-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(C1 -3 alkyl), -C(O)NH2, - C(=0)NH(Ci-3 alkyl), -C(=O)N(C1-3 alkyl)2s -S(=O)2(C,-3alkyl), -S(=O)2NH2, - S(O)2N(C1 -3 alkyl)2, -S(=O)2NH(d-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)πO(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CHa)nO(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined in the above described embodiment.
In a preferred aspect of this embodiment, the invention provides a compound of Formula I for use in treating and/or preventing cancer:
Figure imgf000037_0001
FORMULA 1 wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; or
R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CHi)n-(CH2)Ii-, -(CHi)nNH(CHi)n-, -(CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, and where each n is independently chosen from 0, 1 , 2, and 3; or a pharmaceutically acceptable salt thereof.
In another more preferred aspect, R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyioxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In another more preferred aspect, R8 is -L-heterocyclyl wherein said -L-heterocyclyl is -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido.
In another more preferred aspect, R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents and further wherein said ring or ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, oxadiazolyl, 2,3-dihydrobenzofuranyl, benzo[d][l ,3]dioxolyl,
2,3-dihydrobenzo[b] [l ,4]dioxinyl, 3,4-dihydro-2H-benzo[b] [l ,4]dioxepinyl, and chromanyl.
In another more preferred aspect, R8 is a -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents and further wherein said ring or ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, and oxadiazolyl. In another more preferred aspect, R8 is a -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents and further wherein said ring or ring system is pyridyl.
In another more preferred aspect, R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In another more preferred aspect, R8 is aryl (L is a bond) wherein said aryl has from 1-3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide*, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In another more preferred aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, wherein each n is independently chosen from 0, 1 , 2, and 3. In another more preferred aspect, L is -CH2- or a covalent bond. In another more preferred aspect, L is a covalent bond.
In another more preferred aspect, R6 and R7 are hydro.
In another more preferred aspect, at least one of Rl to R5 is not hydro. In another more preferred aspect, one of R1-R5 is chosen from -L-aryl, -L-heterocyclyl, and -L-carbocyclyl.
In another more preferred aspect, the substituent or the substituents on the R8 ring or ring system are chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(Ci-3 alkyl), -C(=O)NH2, -C(=0)NH(d.3 alkyl), - C(K))N(CL3 alkyl)2, -S(=O)2(Ci-3alkyl), -S(=O)2NH2, -S(O)2N(C1-3 alkyl)2, -SC=O)2NH(C L3 alkyl). -CN, -NH2, and -NO2. In another more preferred aspect, the R8 ring or ring system has 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In another more preferred aspect, the cancer to be treated and/or prevented is chosen from breast cancer, lung cancer, prostate cancer, testicular cancer, brain cancer, colorectal cancer, blood cancer, and skin cancer.
In one embodiment, the invention provides a compound of Formula I:
Figure imgf000041_0001
FORMULA I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl: R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is a -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)I1-(CHi)n-, -(CHi)nNH(CHi)n-, - (CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, and where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof; with the provision that when Rl , R2, R3, R4, R5, R6, and R7 are all hydro and L is a bond, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl, A- methoxyphenyl, 4-chlorophenyl or furan-2-yl, and also when Rl , R2, R3, R4, R5, and R7 are all hydro, R8 is phenyl and L is a bond, then R6 is not methyl, ethyl or isopentyl.
In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined in the above described embodiment.
In one specific aspect of this embodiment the invention provides a compound of Formula I where R8 is -L-heterocyclyl wherein said heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is -L- heteroaryl wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is heteroaryl (L is a bond) wherein said heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CHi)n-(CHi)n-, - (CH2)nNH(CH2)n-, -(CHi)nO(CHi)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CHi)n-(CHi)n- and -(CHi)nO(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CHi-. In a more specific definition, L is a covalent bond.
In one specific aspect of this embodiment the invention provides a compound of Formula I where R8 is -L-aryl wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is aryl (L is a bond) wherein said aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is a phenyl group wherein said phenyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CH2)I1-(CHi)n-, - (CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one specific aspect of this embodiment the invention provides a compound of Formula I where R8 is -L-aryl wherein said aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is aryl (L is a bond) wherein said aryl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido. In an even more specific aspect of this embodiment the invention provides a compound of Formula I where R8 is a phenyl group wherein said phenyl has from 1 -3 (i.e. 1 , 2 or 3) substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido. Unless otherwise specified, according to the aspects described in this paragraph each L is independently chosen from -(CH2)Ii-(CH2)I1-, - (CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific definition, L is chosen from a covalent bond or -CH2-. In a more specific definition, L is a covalent bond.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein the 0-3 substituents or the 1 -3 substituents on the R8 ring or ring system are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C] -3 alkyl)2, -NH(C1 -3 alkyl), -C(=O)NH2, -C(=O)NH(C].3 alkyl), - C(O)N(CL3 alkyl)2, -S(=O)2(C1 -3alkyl), -S(=O)2NH2, -S(O)2N(C1-3 alkyl)2, - S(O)2NH(CL3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In a more specific aspect, the R8 ring or ring system has 1 substituent chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, - NH(Ci-3 alkyl), -C(O)NH2, -C(O)NH(CL3 alkyl), - C(O)N(CL3 alkyl)2, - S(=O)2(C1 -3alkyl), -S(=O)2NH2, -S(O)2N(Cj-3 alkyl)2, - S(=O)2NH(C1 -3 alkyl), - CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In an even more specific aspect, the R8 ring or ring system has from 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2)n- (CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Cj-3 alkyl)2, -NH(C] -3 alkyl), -C(O)NH2, - C(=0)NH(Ci-3 alkyl), -C(=O)N(C1-3 alkyl)2, -S(=O)2(C1-3alkyl), -S(=O)2NH2, - S(O)2N(C1 -3 alkyl)2, -S(=O)2NH(C,-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, RJ is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In a preferred aspect of this embodiment, the invention provides a compound of Formula I:
Figure imgf000047_0001
FORMULA 1 wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino. acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl. arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; or
R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CHi)n-(CHi)n-, -(CHi)nNH(CHi)n-,
-(CHi)nO(CHi)n-, and -(CHi)11S(CHi)11-, and each n is independently chosen from 0,
1 , 2, and 3; or a pharmaceutically acceptable salt thereof; with the provision that when L is a bond and Rl , R2, R3, R4, R5, R6, and R7 are all hydro, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl,
4-methoxyphenyl, 4-chlorophenyl, or furan-2-yl, and also when Rl , R2, R3, R4, R5, and R7 are all hydro, L is a bond and R8 is phenyl, then R6 is not methyl, ethyl, or isopentyl.
In a more preferred aspect, L is independently chosen from -(CHi)n-(CHi)n- and -(CHi)nO(CHi)n-, wherein each n is independently chosen from 0, 1 , 2, and 3. In another more preferred aspect, L is -CHi- or a covalent bond. In another more preferred aspect, L is a covalent bond.
In another more preferred aspect, R6 and R7 are each hydro.
In another more preferred aspect, R8 is an -L-heterocycyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In another more preferred aspect, R8 is an -L-heterocyclyl wherein said -L-heterocycyl is a -L-heteroaryl and wherein the ring or ring system of said - L-heteroaryl has from 0-3 substituents chosen from halo, alky], alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In another more preferred aspect, R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents and further wherein said ring or ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, oxadiazolyl, 2,3-dihydrobenzofuranyl, benzo[d] [l ,3]dioxolyl,
2,3 -dihydrobenzo [b] [ 1 ,4] dioxinyl, 3 ,4-dihydro-2H-benzo [b] [ 1 ,4]dioxepinyl, and chromanyl.
In another more preferred aspect, R8 is a -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents and further wherein said ring or ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, and oxadiazolyl. In another more preferred aspect, R8 is a -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents and further wherein said ring or ring system is pyridyl.
In another more preferred aspect, R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyi, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In another more preferred aspect, R8 is an -L-aryl that has 3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyi, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido.
In another more preferred aspect, one of Rl , R2, R3, R4, and R5 is not a hydro.
In another more preferred aspect, one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl.
In another more preferred aspect, the substituent or the substituents on the R8 ring or ring system are chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1 -3 alkyl)2, -NH(Cj-3 alkyl), -C(=O)NH2, -C(=O)NH(C1 -3 alkyl), -C(=O)N(d-3 alkyl)2, -S(=O)2(d.3alkyl), -S(=O)2NH2, -S(O)2N(Ci-3 alkyl)2, -S(=O)2NH(Ci.3 alkyl), -CN, -NH2, and -NO2.
In another more preferred aspect, the R8 ring or ring system has 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer:
Figure imgf000050_0001
FORMULA I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heteroaryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)I1-(CH2)H-, -(CHa)nNH(CHi)n-, - (CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof; with the provision that when Rl , R2, R3, R4, R5, R6, and R7 are all hydro and L is a bond, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl, 4- methoxyphenyl, 4-chlorophenyl, or furan-2-yl, and also when Rl , R2, R3, R4, R5, and R7 are all hydro, R8 is phenyl and L is a bond, then R6 is not methyl, ethyl or isopentyl.
In one aspect of this embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C! -3 alkyl)2, -NH(Ci-3 alkyl), -C(=O)NH2, - C(O)NH(CL3 alkyl), - C(O)N(CL3 alkyl)2, -S(=O)2(C1.3alkyl), -SO=O)2NH2, - S(O)2N(C1-3 alkyl)2, - S(O)2NH(CL3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In a more specific aspect, the R8 ring or ring system has 1 substituent chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1-3 alkyl)2, -NH(CL3 alkyl), -C(=O)NH2, -C(O)NH(C1 -3 alkyl), - C(O)N(C1 -3 alkyl)2, -S(=O)2(C1_3alkyl), -S(O)2NH2, -S(O)2N(C1 -3 alkyl)2, - S(O)2NH(C1-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In an even more specific aspect, the R8 ring or ring system has from 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 -3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2)n- (CH2),!-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1 -3 alkyl)2, -NH(Cj-3 alkyl), -C(O)NH2, - C(O)NH(C1-3 alkyl), -C(O)N(C1-3 alkyl)2, -S(O)2(C1-3alkyl), -S(O)2NH2, - S(O)2N(C1 -3 alkyl)2, -S(O)2NH(C1 -3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H. In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido;
R6 is chosen from -H and alkyl;
R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl,
N-thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -
(CH2)nO(CH2)n-5 and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof; with the provision that when Rl , R2, R3, R4, R5, R6, and R7 are all hydro and L is a bond, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl, 4- methoxyphenyl, 4-chlorophenyl or furan-2-yl, and also when Rl , R2, R3, R4, R5, and R7 are all hydro, R8 is phenyl and L is a bond, then R6 is not methyl, ethyl or isopentyl.
In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1-3 alkyl)2, -NH(C1 -3 alkyl), -C(=O)NH2, -C(=O)NH(Ci.3 alkyl), - C(=O)N(Ci-3 alkyl)2, -S(=O)2(C1-3alkyl), -S(=O)2NH2, -S(O)2N(C1-3 alkyl)2, - S(O)2NH(Ci-3 alkyl). -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In a more specific aspect, the R8 ring or ring system has 1 substituent chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Cj-3 alkyl)2, - NH(Ci-3 alkyl), -C(O)NH2, -C(O)NH(Ci-3 alkyl), - C(O)N(C1 -3 alkyl)2, - S(O)2(C1-3alkyl), -S(O)2NH2, -S(O)2N(Ci-3 alkyl)2, - S(O)2NH(C1 -3 alkyl), - CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2. In an even more specific aspect, the R8 ring or ring system has from 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2)n- (CH2),,-, -(CH2)πNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1-3 alkyl)2, -NH(C1-3 alkyl), -C(=O)NH2, - C(=O)NH(Ci-3 alkyl), -C(=O)N(C1 -3 alkyl)2, -S(=O)2(Ci-3alkyl), -S(=O)2NH2, - S(O)2N(Cj-3 alkyl)2, -S(=O)2NH(d-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-5 where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one embodiment, the invention provides a compound of Formula I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkyl amino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfmyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and Ci-6 alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is -L-heterocyclyl or -L-aryl wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents wherein said substituents are chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, - N(Ci-3 alkyl)2, -NH(C1-3 alkyl), -C(=O)NH2, -C(=0)NH(d.3 alkyl), - Q=O)N(C1-3 alkyl)2, -S(=O)2(C1 -3alkyl), -S(=O)2NH2, -S(O)2N(C1-3 alkyl)2, -S(=O)2NH(C1-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, - (CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and where each n is independently chosen from O, 1 , 2, and 3 ; and pharmaceutically acceptable salts thereof; with the provision that when Rl , R2, R3, R4, R5, R6, and R7 are all hydro and L is a bond, then R8 is not 2,4-dimεthoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl, 4- methoxyphenyl, 4-chlorophenyl or furan-2-yl, and also when Rl , R2, R3, R4, R5, and R7 are all hydro, R8 is phenyl and L is a bond, then R6 is not methyl, ethyl or isopentyl.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In a more specific aspect of this embodiment, R6 and R7 are each hydro. In an even more specific aspect, Rl, R2, R3, R4, R5, R6, and R7 are each hydro.
In a specific aspect of this embodiment, R8 is a heteroaryl having from 0-3 substituents. In a specific aspect, the heteroaryl ring is chosen from thiazolyl, pyridyl, thiophenyl, and quinolinyl. In one specific aspect, R8 is a heteroaryl that has from 1 to 3 substituents independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(Ci-3 alkyl), - C(=O)NH2, -C(O)NH(Ci-3 alkyl), - C(=0)N(Ci-3 alkyl)2, -S(=O)2(Ci-3alkyl), - S(=O)2NH2, -S(O)2N(Ci-3 alkyl)2, -S(=O)2NH(d-3 alkyl), -CHF2, -OCF3, -OCHF2, - CF3, -CN, -NH2, and -NO2. In a more specific aspect, R8 is a heteroaryl that has from 1 -3 substituents independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl. In one aspect of this embodiment, R8 is not an unsubstituted furan-2-yl or phenyl group when L is a bond.
In one specific aspect of this embodiment, the invention provides a compound of Formula I wherein Rl , R2, R3, R4, R5, R6 and R7 are each hydro and R8 is an -L- aryl group having from 0 to 3 substituents on the ring or ring system of said -L-aryl, the substituents being independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CHi)n-(CHi)n-, -(CHi)nNH(CHi)n-, - (CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, and where each n is independently chosen from 0, 1 , 2, and 3 ; and pharmaceutically acceptable salts thereof; provided that when L is a bond, R8 is not 2,4-dimethoxyphenyl, phenyl, 4-nitrophenyl, A- bromophenyl, 4-methoxyphenyl or 4-chlorophenyl.
In a more specific aspect, R8 is a phenyl group having from 1 to 3 substituents. In a more specific aspect, the substituents on the R8 phenyl ring are chosen from halo, alkyl, alkoxy, cycloalkoxy, cyano, haloalkyl and hydroxyl. In another more specific aspect the substituents on the R8 ring are chosen from -F, -Cl, -Br, -CH3, -OH, -
CF3, cyano, and -OCH3.
Each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -
(CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, where each n is independently chosen from 0,
1 , 2, and 3 ; and pharmaceutically acceptable salts thereof; provided that when L is a bond, R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, A- bromophenyl, 4-methoxyphenyl or 4-chlorophenyl. In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
In one embodiment, the invention provides a compound of Formula I wherein: one of R1 -R5 is chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; and the others of R1 -R5 are independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-5 - (CH2)nO(CH2)n-, and -(CHi)nS(CHi)n-, and where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, aryl alkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2)n- (CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1 -3 alkyl)2, -NH(C1 -3 alkyl), -C(=O)NH2, - C(=O)NH(Ci-3 alkyl), -C(O)N(C1-3 alkyl)2, -S(=O)2(C]-3alkyl), -S(=O)2NH2, - S(O)2N(C1-3 alkyl)2, -S(=O)2NH(Ci-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)I1-(CHi)n- and -(CHi)nO(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein one of R1-R5 is a substituent chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; and the others of R1 -R5 are each hydro; R6 and R7 are each hydro; and
R8 is chosen from aryl or heterocyclyl wherein the ring or ring system of said aryl or heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CHi)n-(CHi)n-, -(CHi)nNH(CHi)n-, - (CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, and where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof.
In one aspect of this embodiment, the ring or ring system of R8 has 1 -3 substituents as described and defined above. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents as defined above. In an even more specific aspect, the ring or ring system of R8 has 1 substituent as defined above.
In one specific aspect of this embodiment, R8 has from 1 -3 substituents independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C-3 alkyl)2, -NH(Ci-3 alkyl), -C(O)NH2, -C(O)NH(C 1.3 alkyl), - C(O)N(CL3 alkyl)2, -S(O)2(CL3 alkyl), -S(=O)2NH2, -S(O)2N(C1 -3 alkyl)2, - S(O)2NH(CL3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2.
In one specific aspect of this embodiment, the one of R1-R5 is chosen from -L-aryl, -L-heterocyclyl, and -L-carbocyclyl. In an even more specific aspect, the one of Rl- R5 is -L-aryl, In an even more specific aspect, the one of R1 -R5 is benzyloxy.
In one specific aspect of this embodiment, the substituent(s) on R8 are independently chosen from halo, alkyl, alkoxy, cycloalkoxy, cyano, haloalkyl, and hydroxyl.
In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
In one embodiment, the invention provides a compound of Formula I wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is -H; R7 is -H; R8 is chosen from -L-heterocyclyl and -L-aryl, wherein wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, - (CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-5 and where each n is independently chosen from 0, 1 , 2, and 3 ; and pharmaceutically acceptable salts thereof; with the provision that when Rl , R2, R3, R4, and R5 are all hydro and L is a bond, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, 4-bromophenyl, 4- methoxyphenyl, 4-chlorophenyl, or furan-2-yl.
In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 -3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2)n- (CHa)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Cj-3 alkyl)2, -NH(Cj-3 alkyl), -C(=O)NH2, - C(=O)NH(Ci-3 alkyl), -C(=O)N(Ci-3 alkyl)2, -S(=O)2(C1-3alkyl), -S(=O)2NH2, - S(O)2N(Ci-3 alkyl)2, -S(=O)2NH(Ci-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from halo, alkoxy, cycloalkoxy, cyano, and alkyl. In a more specific aspect the R8 ring or ring system has 1 or 2 substituents independently chosen from halo, alkoxy, cycloalkoxy, cyano, and alkyl. In an even more specific aspect, the R8 ring or ring system has 1 substituent independently chosen from halo, alkoxy, cyano, and alkyl.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from -Cl, -Br, -F, cyano, and methoxy. In a more specific aspect the R8 ring or ring system has 1 or 2 substituents indepependently chosen from -Cl, -Br, -F, cyano, and methoxy. In an even more specific aspect, the R8 ring or ring system has 1 substituent independently chosen from -Cl, -Br, -F, cyano, and methoxy.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein R8 is chosen from phenyl, thiazolyl, pyridyl, thiophenyl. and quinolinyl. In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
In one embodiment, the invention provides compounds of Formula I wherein each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, - (CHi)nO(CH2)I1-, and -(CH2)IiS(CHi)n-, and where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof; with the provision that when Rl to R7 are all hydro and L is a bond, then R8 is not an unsubstituted phenyl group, a 4-halophenyl group, a 4-methoyxphenyl group, a 2,4- dimethoxyphenyl group, a 4-nitrophenyl group or a furanyl group, and also when Rl to R5 and R7 are all hydro, R8 is phenyl and L is a bond, then R6 is not methyl, ethyl or isopentyl. In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, the ring or ring system of R8 has 1-3 substituents as described and defined above. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents as defined above. In an even more specific aspect, the ring or ring system of R8 has 1 substituent as defined above.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein R8 is aryl wherein the ring or ring system of said aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein R8 is heteroaryl wherein the ring or ring system of said heteroaryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein R8 is heterocyclyl wherein the ring or ring system of said heterocyclyl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein R8 is chosen from phenyl, pyridinyl, thiazolyl, and thiophenyl, wherein the ring of said group has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein the substituents on the R8 ring or ring system are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Cj-3 alkyl)2, -NH(Ci-3 alkyl), -C(O)NH2, -C(O)NH(CL3 alkyl), - C(O)N(CM alkyl)2, - S(=O)2(Ci-3alkyl), -S(O)2NH2, -S(O)2N(Ci-3 alkyl)2, - S(O)2NH(Ci-3 alkyl), - CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, and -NO2.
In one aspect of this embodiment, the invention provides a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from halo, alkoxy, cycloalkoxy, cyano, and alkyl. In a more specific aspect, the R8 ring or ring system has 1 or 2 substituents independently chosen from halo, alkoxy, cycloalkoxy, cyano, and alkyl. In an even more specific aspect, the R8 ring or ring system has 1 substituent chosen from halo, alkoxy, cyano, and alkyl. In one aspect of this embodiment, the invention provides a compound of Formula I wherein the 0-3 substituents on the R8 ring or ring system are independently chosen from -Cl, -Br, -F, cyano, and methoxy. In a more specific aspect the R8 ring or ring system has 1 or 2 substituents independently chosen from -Cl, -Br, -F, cyano, and methoxy. In an even more specific aspect, the R8 ring or ring system has 1 substituent chosen from -Cl, -Br, -F, cyano, and methoxy.
In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
In one embodiment, the invention provides a compound of Formula I wherein Rl , R2, R3, R4, and R5 are each hydro; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is chosen from -L-heterocyclyl and -L-aryl wherein the ring or ring system of said -L-heterocyclyl or -L-aryl has from 0-3 substituents independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, aryl alkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, - (CHa)nO(CH2)I1-, and -(CHi)nS(CHi)n-, and where each n is independently chosen from 0, 1 , 2, and 3; and pharmaceutically acceptable salts thereof, with the provision that when R6 and R7 are both hydro and L is a bond, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl, 4-methoxyphenyl, A- chlorophenyl, or furan-2-yl, and also when R7 is hydro, L is a bond and R8 is phenyl, then R6 is not methyl, ethyl or isopentyl. In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2)n- (CHz)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)I1S(CH2)11-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(C1 -3 alkyl)2, -NH(Ci-3 alkyl), -C(=O)NH2, - C(=O)NH(Ci-3 alkyl), -C(O)N(Ci-3 alkyl)2, -S(=O)2(Ci-3alkyl), -SC=O)2NH2, - S(O)2N(C1 -3 alkyl)2, -S(=O)2NH(Ci-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from O, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
In one embodiment, the invention provides a compound of Formula I wherein one of Rl , R2, R3, R4, and R5 is chosen from halo, alkyl, alkoxyl, haloalkyl, haloalkoxy, cyano, amino, alkylamino, -L-heterocyclyl, -L-aryl, and -L-carbocyclyl; and the others of Rl, R2, R3, R4, and R5 are chosen from -H, halo, alkyl, alkoxyl, haloalkyl, haloalkoxy, cyano, amino, alkylamino, -L-hetεrocyclyl, -L-aryl, and -L- carbocyclyl;
R6 is chosen from -H and alkyl;
R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is chosen from heteroaryl and aryl, wherein the ring or ring system of said heteroaryl or aryl has from 0-3 substituent independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heteroaryl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CHa)n-(CH2)Ii-, -(CH2)nNH(CH2)n-, -
(CH2)nO(CH2)n-5 and -(CH2)nS(CH2)n-, and where each n is independently chosen from 0, 1 , 2, and 3 ; and pharmaceutically acceptable salts thereof.
In one aspect of this embodiment, R7 is chosen from -H and cycloalkyl. In a more specific aspect of this embodiment, R7 is -H.
In one aspect of this embodiment, the ring or ring system of R8 has 0-3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 -3 substituents. In a more specific aspect, the ring or ring system of R8 has 1 or 2 substituents. In an even more specific aspect, the ring or ring system of R8 has 1 substituent. In a further aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2V (CH2V, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2V, and -(CH2)nS(CH2)n-, and further wherein each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect of this embodiment, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(C1 -3 alkyl), -C(=O)NH2, - C(=O)NH(Ci-3 alkyl), -C(=O)N(C1 -3 alkyl)2, -S(=O)2(C,-3alkyl), -S(=O)2NH2, - S(O)2N(Ci-3 alkyl)2, -S(=O)2NH(C1-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, the aforementioned substituent(s) on the ring or ring system of R8 are/is independently chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
In one aspect of this embodiment, L is independently chosen from -(CH2V(CH2V, -(CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and -(CH2)nS(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, where each n is independently chosen from 0, 1 , 2, and 3. In a more specific aspect, L is chosen from a covalent bond or -CH2-. In an even more specific aspect, L is a covalent bond.
In one specific aspect of this embodiment, the invention provides a compound of Formula I wherein one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, - L-aryl, and -L-carbocyclyl. In a more specific aspect of this embodiment, one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl, and the others are hydro. In an even more specific aspect L is a covalent bond.
In one specific aspect of this embodiment, the invention provides a compound of Formula I wherein R6 and R7 are hydro, and one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl. In a more specific aspect of this embodiment, R6 and R7 are hydro, one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl, and the others of Rl to R5 are hydro. In an even more specific aspect L is a covalent bond.
In one aspect of this embodiment, the invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I as defined above.
Furthermore, in one aspect of all the embodiments provided herein, the ring or ring system of R8 has at least one substituent chosen from the substituents described and defined in the respective embodiments.
In one aspect of all the embodiments provided herein, the ring or ring system of R8 has three substituents chosen from the substituents described and defined in the respective embodiments. For example, said three substituents may be independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido.
In one aspect of all the embodiments provided herein, R8 is aryl (such as, e.g., phenyl) or heterocyclyl (such as, e.g., pyridinyl, thiazolyl, or thiophenyl), wherein the ring or ring system of said aryl has 1 -3 substituents (such as, e.g., 1 or 2 substituents) and further wherein the ring or ring system of said heterocyclyl has from 0-3 substituents (such as, e.g., 1 or 2 substituents), said substituents being independently chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, - L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido. In a more specific aspect, said substituents are independently chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Cj-3 alkyl)2, -NH(Ci-3 alkyl), -C(=O)NH2, - C(=O)NH(Ci-3 alkyl), - C(=O)N(C1-3 alkyl)2, -S(=O)2(C,-3alkyl), -S(=O)2NH2, - S(O)2N(C1 -3 alkyl)2, - S(=O)2NH(d-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, - NH2, and -NO2. In an even more specific aspect, said substituents are independently chosen from halo, alkyl, alkoxy, cycloalkoxy, and cyano.
In one specific aspect of all the embodiments provided herein, one of R1 -R5 is chosen from -L-aryl, -L-heterocyclyl, and -L-carbocyclyl. In a more specific aspect, one of R1-R5 is -L-aryl. In an even more specific aspect, one of R1 -R5 is benzyloxy.
In a further aspect of all the embodiments provided herein, R3 is benzyloxy and each of Rl , R2, R4 and R5 is hydro.
In one aspect of all the embodiments provided herein, a compound wherein Rl to R7 are hydro and R8 is unsubstituted phenyl is excluded.
In one preferred aspect of all the embodiments provided herein, when the ring or ring system of R8 is a heterocycyl said ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, oxadiazolyl, 2,3-dihydrobenzofuranyl, benzo[d] [l ,3]dioxolyl, 2,3-dihydrobenzo[b] [l ,4]dioxinyl, 3,4-dihydro-2H- benzo[b] [l ,4]dioxepinyl, and chromanyl.
In one preferred aspect of all the embodiments provided herein, when the ring or ring system of R8 is a heteroaryl said ring system is chosen from pyridyl, thiazolyl, thiophenyl, quiolinyl, indolyl, and oxadiazolyl. In an even more preferred aspect R8 is pyridyl.
In one embodiment, the invention provides a compound of Formula I (or a pharmaceutically acceptable salt thereof), a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Formula I (or a pharmaceutically acceptable salt thereof), and a compound of Formula I (or a pharmaceutically acceptable salt thereof) for use in treating or preventing cancer, wherein: Rl to R7 are hydro and R8 is phenyl having 1 , 2 or 3 substituents chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(C1 -3 alkyl), -C(=O)NH2, -C(O)NH(C1 -3 alkyl), -C(O)N(C1-3 alkyl)2, - S(=O)2(C1-3 alkyl), -S(O)2NH2, -S(O)2N(C1-3 alkyl)2, - S(=O)2NH(C1-3 alkyl), -CN, -NH2, and -NO2.
In one aspect of all the embodiments provided herein, the compound of Formula I is chosen from:
(trans)-N-(4-fluorobenzyl)-2-phenylcyclopropanamine;
(trans)-N-(4-fluorobenzyl)-2-phenylcyclopropanaminium;
4-(((trans)-2-phenylcyclopropylamino)methyl)benzonitrile;
(trans)-N-(4-cyanobenzyl)-2-phenylcyclopropanaminium;
(trans)-2-phenyl-N-(4-(trifluoromethyl)benzyl)cyclopropanamine;
(trans)-2-phenyl-N-(4-(trifluoromethyl)benzyl)cyclopropanaminium;
(trans)-2-phenyl-N-(pyridin-2-ylmethyl)cyclopropanamine;
(trans)-2-phenyl-N-(pyridin-3-ylmethyl)cyclopropanamine;
(trans)-2-phenyl-N-(pyridin-4-ylmethyl)cyclopropanamine;
(trans)-N-((6-methylpyridin-2-yl)methyl)-2-phenylcyclopropanamine;
(trans)-2-phenyl-N-(thiazol-2-ylmethyl)cyclopropanamine;
(trans)-2-phenyl-N-(thiophen-2-ylmethyl)cyclopropanamine;
(trans)-N~((3-bromothiophen-2-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((4-bromothiophen-2-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(3,4-dichlorobenzyl)-2-phenylcyclopropanamine;
(trans)-N-(3-fluorobenzyl)-2-phenylcyclopropanaminium;
(trans)-N-(2-fluorobenzyl)-2-phenylcyclopropanamine; (trans)-2-phenyl-N-(quinolin-4-ylmethyl)cyclopropanamine;
(trans)-N-(3-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-2-phenyl-N-((6-(trifluoromethyl)pyridin-3- yl)methyl)cyclopropanamine;
(trans)-N-((6-chloropyridin-3-yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-((4-methylpyridin-2-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((6-methoxypyridin-2-yl)methyl)-2-phenylcyclopropanamine;
2-(((trans)-2-phenylcyclopropylamino)methyl)pyridin-3-ol;
(trans)-N-((6-bromopyridin-2-yl)methyl)-2-phenylcyclopropanamine;
4-(((trans)-2-(4-(benzyloxy)phenyl)cyclopropylamino)methyl)benzonitrile;
(trans)-N-(4-(benzyloxy)benzyl)-2-phenylcyclopropanamine;
(trans)-N-benzyl-2-(4-(benzyloxy)phenyl)cyclopropanamine;
(trans)-2-(4-(benzyloxy)phenyl)-N-(4- methoxybenzyl)cyclopropanamine;
(trans)-2-(4-(benzyloxy)phenyl)-N-(4- fluorobenzyl)cyclopropanamine; and pharmaceutically acceptable salts thereof.
Accordingly, the invention provides a compound of Formula I chosen from the above compounds, in particular for use as a medicament and also for use in treating and/or preventing cancer.
In one aspect of all the embodiments provided herein, the compound of Formula I is chosen from:
(trans)-2-phenyl-N-(quinolin-2-ylmethyl)cyclopropanamine;
(trans)-2-phenyl-N-((5-(trifluoromethyl)pyridin-2-yl)methyl)cyclopropanamine;
(trans)-N-((3-fluoropyridin-2-yl)methyl)-2-phenylcyclopropanamine;
(trans)-2-phenyl-N-(quinolin-3-ylmethyl)cyclopropanamine;
(trans)-N-((6-methoxypyridin-3-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((5-methoxypyridin-3-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((2-methoxypyridin-3-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((3H-indol-3-yl)methyl)-2-phenylcyclopropanamine;
3-(((trans)-2-phenylcyclopropylamino)methyl)benzonitrile;
(trans)-N-(2-methoxybenzyl)-2-phenylcyclopropanamine;
3-(((trans)-2-phenylcyclopropylamino)methyl)pyridin-2-amine
(trans)-N-((2-chloropyridin-3-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(3,4-dimethoxybenzyl)-2-phenylcyclopropanamine; (trans)-N-((2,3-dihydrobenzofuran-5-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(benzo[d][l ,3]dioxol-5-ylmethyl)-2-phenylcyclopropanamine;
(trans)-N-((2,3-dihydrobenzo[b] [l ,4]dioxin-6-yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-(2,6-difluoro-4-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-2-phenyl-N-(4-(trifluoromethoxy)benzyl)cyclopropanamine;
(trans)-N-(5-fluoro-2-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2-fluoro-4-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-((4-methoxynaphthalen-l -yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(2-fluoro-6-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-((2-methoxynaphthalen-l -yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((4,7-dimethoxynaphthalen- l -yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(4-methoxy-3-methylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-(3-chloro-4-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(3-fluoro-4-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(4-methoxy-2-methylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-((3,4-dihydro-2H-benzo[b] [l ,4]dioxepin-6-yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-((3,4-dihydro-2H-benzo[b][l ,4]dioxepin-7-yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-((2,2-dimethylchroman-6-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(4-methoxy-2,3-dimethylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-(4-methoxy-2,5-dimethylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2-fluoro-4,5-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(3-chloro-4,5-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2-chloro-3,4-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2,4-dimethoxy-6-methylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2,5-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2,3-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2-chloro-3-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-((lH-indol-5-yl)methyl)-2-phenylcyclopropanamine;
(trans)-2-(4-(benzyloxy)phenyl)-N-(pyridin-2-ylmethyl)cyclopropanamine;
(trans)-2-(4-(benzyloxy)phenyl)-N-(2-methoxybenzyl)cyclopropan amine; (trans)-N-(l-(4-methoxyphenyl)ethyl)-2-phenylcyclopropan amine;
(trans)-N-(l -(3,4-dimethoxyphenyl)ethyl)-2-phenylcyclopropanamine;
(trans)-N-(l -(2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)ethyl)-2- phenylcyclopropanamine;
(trans)-N-(l -(5-fluoro-2-methoxyphenyl)ethyl)-2-phenylcyclopropanamine;
(trans)-N-(l -(3,4-dimelhoxyphenyl)propan-2-yl)-2-phenylcyclopropanamine;
(trans)-N-((3-methyl-1.2,4-oxadiazol-5-yl)methyl)-2-phenylcyclopropanamine; and pharmaceutically acceptable salts thereof.
Accordingly, the invention provides a compound of Formula I chosen from the above compounds, in particular for use as a medicament and also for use in treating and/or preventing cancer.
The invention provides compounds of Formula I which are selective inhibitors of LSD l . LSDl selective inhibitors have IC50 values for LSDl which are at least 2- fold lower than the IC50 value for MAO-A and/or MAO-B. In some embodiments, the LSDl selective inhibitors have IC50 values which are at least 5-fold lower for LSDl as compared to MAO-A and MAO-B. In some embodiments, the LSDl selective inhibitors have IC50 values which are at least 10-fold lower for LSDl as compared to MAO-A and MAO-B.
The invention also provides compounds of Formula I which are dual inhibitors selective for LSDl and MAO-B. Dual LSD1/MA0-B selective inhibitors have IC50 values for LSDl and MAO-B which are at least 2-fold lower than the IC50 value for MAO-A. In some embodiments, the dual LSD 1/MA0-B selective inhibitors have IC50 values which are at least 5-fold lower for LSDl and MAO-B as compared to MAO-A. In some embodiments, the dual LSD1/MA0-B selective inhibitors have IC50 values which are at least 10-fold lower for LSDl and MAO-B as compared to MAO-A. In one aspect of this embodiment, the LSD1/MA0-B inhibitor avoids the deleterious side-effects asscoaited with inhibition of MAO-A.
In one embodiment, the invention provides a method of treating a cancer comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing cancer. In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for treating and/or preventing cancer.
Previous reports of LSDl have found that it is involved in cell proliferation and growth. Some studies have implicated LSDl as a therapeutic target for cancer. Huang et al. (2007) PNAS 104:8023-8028 found that polyamines inhibitors of LSD l modestly cause the reexpression of genes aberrantly silenced in cancer cells and particularly colorectal cancer (Huang et al. Clin Cancer Res. (2009) Dec l ; 15(23):7217-28. Epub 2009 Nov 24. PMID: 19934284). Scoumanne et al ((2007) J. Biol. Chem. May 25;282(21): 15471-5) found that deficiency in LSDl leads to a partial cell cycle arrest in G2/M and sensitizes cells to growth suppression induced by DNA damage. Kahl et al. ((2006) Cancer Res. 66(23): 11341 -7.) found that LSDl expression is correlated with prostate cancer aggressiveness. Metzger et al. reported that LSDl modulation by siRNA and pargyline regulates androgen receptor (AR) and may have therapeutic potential in cancers where AR plays a role, like prostate, testis, and brain cancers. Lee et al. ((2006) Chem. Biol. 13 :563-567) reported that tranylcypromine derepresses Egr-1 gene expression in some cancer lines. A body of evidence is accumlating that Egr-1 is a tumor suppressor gene in many contexts (see e.g., Calogero el al. (2004) Cancer Cell International 4: 1 exogenous expression of EGR-1 resulted in growth arrest and eventual cell death in primary cancer cell lines; Lucerna et al. (2006) Cancer Research 66, 6708-6713 show that sustained expression of Egr-1 causes antiangiogeneic effects and inhibits tumor growth in some models; Ferraro et al. ((2005) J. Clin. Oncol. Mar 20;23(9): 1921 -6) reported that Egr-1 is downregulated in lung cancer patients with a higher risk of recurrence and may be more resistant to to therapy. Thus, increasing Egr-1 expression via inhibition of LSDl is a therapeutic approach for some cancers. Recent studies have also implicated LSD l in brain cancer (Schulte et al. (2009) Cancer Res. Mar l ;69(5):2065-71). Other studies have implicated LSD l in breast cancer (Lims et al. Carcinogenesis. 2009 Dec 30. [Epub ahead of print] PMID: 20042638). Thus, a body of evidence has implicated LSD l in a number of cancers, which suggests that LSD l is a therapeutic target for cancer. The instant inventors have discovered a class of LSDl inhibitors that can be used to treat diseases where LSDl is implicated as a therpautic target like cancer. Accordingly, the phenylcyclopropylamine compounds of the invention can be used to treat and/or prevent such diseases.
As the skilled artisan readily recognizes the compounds disclosed herein are surprisingly and significantly more potent than tranylcypromine for LSDl inhibition. Han et al. (Euro. J. Pharma. (2008) doi: 10.1016/j.ejphar.2008.12.025) reported that phenylcyclopropylamine displays neuroprotective activity in PC 12 cells thus the compounds of Formula I may be used as a neuroprotectant (e.g., used to treat and/or prevent conditions characterized by neurodegeneration). Furthermore, since the compounds of Formula I are potent LSDl inhibitor they can be used to treat and/or prevent diseases where LSDl inhibition is desirable, e.g., cancer.
Without being bound by theory, it is believed that compounds provided herein are particularly useful in the treatment and/or prevention of cancer because they interfere with LSDl activity (i.e. inhibit LSDl activity), wherein LSDl activity is part of a metabolic mechanism specifically involved in cancer as described elsewhere herein. Potent LSD l inhibitors to be used in the treatment of cancer are shown in Table 1 , wherein compounds with a low IC50 value (in particular compounds having a lower IC50 value than the prior art compound parnate) are preferred.
Furthermore, and without being bound by theory, dual MAO-B/LSD1 inhibitors provided herein are thought of interfering with a mechanism involved in neurodegenerative diseases. Accordingly, compounds that potently inhibit both MAO-B and LSDl activity are preferred in the treatment of neurodegenerative diseases. Exemplary compounds in this context are shown in Table 1 , wherein compounds with a low IC50 value (in particular compounds having a lower IC50 value than the prior art compound parnate) are preferred. Also compounds that inhibit both MAO A/B acitivy and LSDl activity are envisaged herein in the treatment of diseases, in particular neurodegenerative diseases, wherein compounds that inhibit LSDl activity more potently than parnate are preferred.
In one embodiment, the invention provides a method of inhibiting LSD l activity comprising administering, to a patient in need of treatment, an amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier sufficient to inhibit LSDl activity. In a related embodiment, the invention provides a compound of Formula I for inhibiting LSDl . In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for inhibiting LSDl .
In one embodiment, the invention provides a method of treating a neurodegenerative disease or disorder comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing a neurodegenerative disease. In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for treating and/or preventing a neurodegenerative disease.
In one embodiment, the invention provides a method of inhibiting monoamine oxidase activity comprising administering, to a patient in need of treatment, an amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier sufficient to inhibit monoamine oxidase activity. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing Parkinson's disease and/or depression. In another related embodiment, the invention provides for the use of a compound of Formula I for the manufacture of a medicament for inhibiting monoamine oxidase. In a related embodiment, the invention provides a compound of Formula I for treating and/or preventing neurogeneration.. In some of the embodiments related to Formula I, the compound does not have the structure of the compounds having CAS registration nos. 22783-31-9, 1041848-33-2, 903487-42-3, 53578-57-7, 53578-56-6, 53578-54-4, 53578-53-3, 53578-52-2, 53578-50-0, 39933-77-2, 32752-01 -5, and/or 22783-27-3.
In one embodiment, the invention provides a method of treating a disease or condition comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier. In one aspect of this embodiment, the disease is cancer or a neurodegenerative disease.
In one embodiment, the invention provides a method of treating a cancer comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier. In one aspect of this embodiment, the cancer is prostate cancer. In another specific aspect of this embodiment, the cancer is brain cancer. In yet another specific aspect of this embodiment, the cancer is breast cancer. In yet another specific aspect of this embodiment, the cancer is lung cancer. In yet another specific aspect of this embodiment, the cancer is testicular cancer. In yet another specific aspect of this embodiment, the cancer is colorectal cancer. In yet another specific aspect of this embodiment, the cancer is blood cancer (e.g., leukemia). In yet another specific aspect of this embodiment, the cancer is skin cancer.
In one embodiment, the invention provides a method of treating a neurodegenerative disease or disorder comprising administering, to a patient in need of treatment, a therapeutically effective amount of a composition comprising a compound of Formula I and a pharmaceutically acceptable carrier.
In one embodiment, the invention provides a method for identifying LSDl selective inhibitors comprising determining the ability of a test compound to inhibit LSDl and monoamine oxidase (MAO-A and/or MAO-B) wherein a test compound that inhibits LSD l better than monoamine oxidase (MAO-A and/or MAO-B) is LSD l selective. In one aspect of this embodiment, the selective inhibitor has an IC50 value at least two-fold lower for LSDl as compared to MAO-A and MAO-B. In one aspect of this embodiment, the LSD l selective inhibitor has an IC50 value at least five-fold lower for LSDl as compared to MAO-A and MAO-B. In one aspect of this embodiment, the LSDl selective inhibitor has an IC50 value at least ten-fold lower for LSD l as compared to MAO-A and MAO-B. In one aspect of this embodiment, the test compound is chosen from a phenylcyclopropylamine derivative, homolog or analog. In one specific aspect of this embodiment the phenylcyclopropylamine analog has the phenyl group replaced with another ring system (e.g., aryl, heterocyclyl and/or heteroaryl which is optionally substituted) and the amine group is substituted with a functional group (see e.g., examples). In another aspect, the test compound is a phenylcyclopropylamine analog or derivative where the amine group is substituted with a functional group. In another aspect, the test compound is a phenylcyclopropylamine analog or derivative where the amine group is substituted with a functional group and the phenyl group has at least one substituent that is not a hydrogen atom. In a related embodiment, the invention provides a method of inhibiting LSDl selectively as compared to MAO-B and MAO-A comprising administering to an individual a phenylcyclopropylamine derivative, homolog, or analog that selectively inhibits LSDl . In a related embodiment, the invention provides a method of inhibiting LSDl selectively as compared to MAO-B and MAO- A comprising administering to an individual a compound of Formula I as defined in the embodiments described in this invention, that selectively inhibits LSDl .
In one embodiment, the invention provides a method for identifying a dual inhibitor selective for LSDl /MAO-B as compared to MAO-A comprising determining the ability of a test compound to inhibit LSDl and monoamine oxidase (MAO-A and MAO-B) wherein a test compound that inhibits LSDl and MAO-B better than LSDl and monoamine oxidase (MAO-A and/or MAO-B) is a dual LSDl /MAO-B selective inhibitor. In one aspect of this embodiment, the dual LSDl /MAO-B selective inhibitor has an IC50 value at-least two-fold lower for LSDl and MAO-B as compared to MAO-A. In one aspect of this embodiment, the dual LSDl /MAO-B selective inhibitor has an IC50 value at-least five-fold lower for LSDl and MAO-B. In one aspect of this embodiment, the dual LSD 1/MA0-B selective inhibitor has an IC50 value at-least ten-fold lower for LSD l and MAO-B as compared to MAO-A. In one aspect of this embodiment, the test compound is chosen from a phenylcyclopropylamine derivative, homolog or analog. In one specific aspect of this embodiment the phenylcyclopropylamine analog has the phenyl group replaced with another ring system (e.g., aryl, heterocyclyl and/or heteroaryl which is optionally substituted) and the amine group is substituted with a functional group (see e.g., examples). In another aspect, the test compound is a phenylcyclopropylamine analog or derivative where the amine group is substituted with a functional group. In another aspect, the test compound is a phenylcyclopropylamine analog or derivative where the amine group is substituted with a functional group and the phenyl group has at-least one substituent that is not a hydrogen. In a related embodiment, the invention provides a method of inhibiting LSD l and MAO-B selectively as compared to MAO-A comprising administering to an individual a phenylcyclopropylamine derivative, homolog, or analog that selectively inhibits LSDl and MAO-B. In a related embodiment, the invention provides a method of inhibiting LSDl and MAO-B selectively as compared to MAO- A comprising administering to an individual a compound of Formula I as defined in the embodiments described in this invention, and that selectively inhibits LSDl and MAO-B.
Phenylcyclopropylamine derivatives, homologs, and analogs can be made by methods known in the art e.g., including, but not limited to the methods disclosed herein and in the references cited herein. See for example, Kaiser et al. (1962) J. Med. Chem. 5: 1243-1265 and Zirkle et al. (1962) J. Med. Chem. 1265-1284 Yoshida et al. (2004) Bioorg. Med Chem. 12(10):2645-2652; Hruschka et al. (2008) Biorg Med Chem. (16):7148-7166; and Gooden et al. (2008) Bioorg. Med. Chem. Let. 18:3047-3051 which describe various phenylcyclopropropylamine analogs, derivatives and homologs. The amine groups of these compounds can be alkylated as described in the examples to give substituted phenylcyclopropylamine derivative, analogs, and homologs.
Thus, in a specific embodiment, the invention provides a method of identifying LSD l selective and LSD1/MAO-B inhibitors comprising determining the ability of a test compound to inhibit LSDl , MAO-A, and MAO-B wherein a LSDl selective inhibitor is identified when the test compound inhibits LSDl to a greater extent than MAO-A and MAO-B and wherein a LSDl /MAO-B dual inhibitor is identified when the test compound inhibits LSDl and MAO-B to a greater extent than it inhibits MAO-A wherein said test compound is compound of Formula I:
Figure imgf000083_0001
FORMULA I wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido;
R6 is chosen from -H and alkyl;
R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is -L-heterocyclyl or -L-aryl, wherein the ring or ring system of said -L- heterocyclyl or -L-aryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -
(CHi)nO(CH2)I1-, and -(CH2)I1S(CH2)Ii-, where each n is independently chosen from 0,
1 , 2, and 3; or a compound of Formula I where the phenyl ring attached to the cyclopropylamine moiety is replaced by an optionally substituted heteroaryl group and the other variables R6-R8 as as defined above.
Compounds that are identified as LSDl selective and LSDl /MAO-B dual inhibitors can be used to prepare pharmaceutical compositions for treating diseases according to the methods of the invention as described herein. Preferably the diseases can be treated by inhibiting LSDl , LSDl and MAO-B, and/or by modulating histone methylation levels.
In a specific aspect of this embodiment, the invention provides a method of identifying LSDl selective and LSDl /MAO-B dual inhibitors comprising determining the ability of a test compound to inhibit LSD l , MAO-A, and MAO-B wherein a LSDl selective inhibitor is identified when the test compound inhibits LSDl to a greater extent than MAO-A and MAO-B and wherein a LSDl /MAO-B dual inhibitor is identified when the test compound inhibits LSDl and MAO-B to a greater extent than it inhibits MAO-A wherein said test compound is compound of Formula I:
Figure imgf000084_0001
FORMULA I wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl;
R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N- carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; or
R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L- aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)H-(CHi)n-, -(CHi)nNH(CHi)n-,
-(CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, where each n is independently chosen from
0, 1 , 2, and 3; or a compound of Formula I where the phenyl ring attached to the cyclopropylamine moiety is replaced by an optionally substituted heteroaryl group and the other variables R6-R8 are as defined above.
As used herein, the term "alkyl" refers to a saturated aliphatic hydrocarbon including straight chain and/or branched chain groups. In a more specific definition, the alkyl group is further defined as having 1 to 20 carbon atoms (whenever it appears herein, a numerical range such as " 1 to 20" refers to each integer in the given range; e.g., " 1 to 20 carbon atoms" means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc. up to and including 20 carbon atoms). In another more specific definition, it is an alkyl having 1 to 10 carbon atoms. In yet another more specific definition, it is an alkyl having 1 to 6 carbon atoms, and in yet another more specific definition, it is an alkyl having 1 to 4 carbon atoms.
As used herein, the term "alkenyl" refers to an unsaturated hydrocarbon including straight chain and/or branched chain groups, comprising at least one carbon-to- carbon double bond. In a more specific definition, the alkenyl group is further defined as having 2 to 20 carbon atoms. In another more specific definition, it is an alkenyl having 2 to 10 carbon atoms. In yet another more specific definition, it is an alkenyl having 2 to 6 carbon atoms, and in yet another more specific definition, it is an alkenyl having 2 to 4 carbon atoms.
As used herein, the term "alkynyl" refers to an unsaturated hydrocarbon including straight chain and/or branched chain groups, comprising at least one carbon-to- carbon triple bond. In a more specific definition, the alkynyl group is further defined as having 2 to 20 carbon atoms. In another more specific definition, it is an alkynyl having 2 to 10 carbon atoms. In yet another more specific definition, it is an alkynyl having 2 to 6 carbon atoms, and in yet another more specific definition, it is an alkynyl having 2 to 4 carbon atoms.
As used herein, the term "halo" refers to a group chosen from chloro, fluoro, bromo, and iodo.
As used herein, the term "hydro" refers to a hydrogen atom (-H group) which is also denoted as -H.
As used herein, the term "hydroxyl" refers to an -OH group.
As used herein, the term "alkoxy" refers to an -O-alkyl group, wherein the alkyl group is as defined herein above.
As used herein, the term "cycloalkoxy" refers to an -O-cycloalkyl group, wherein the cycloalkyl group is as defined herein below. As used herein, the term "aryloxy" refers to an -O-aryl group, wherein the aryl group is as defined herein below.
As used herein, the term "heteroaryloxy" refers to an -O-heteroaryl group, wherein the heteroaryl group is as defined herein below.
As used herein, the term "mercapto" refers to an -SH group.
As used herein, the term "alkylthio" refers to an -S-alkyl group, wherein the alkyl group is as defined herein above.
As used herein, the term "cycloalkylthio" refers to an -S-cycloalkyl group, wherein the cycloalkyl group is as defined herein below.
As used herein, the term "arylthio" refers to an -S-aryl group, wherein the aryl group is as defined herein below.
As used herein, the term "heteroarylthio" refers to an -S-heteroaryl group, wherein the heteroaryl group is as defined herein below.
As used herein, the term "carbonyl" refers to a -C(=O)R" group, wherein R" is as defined herein below.
Unless specified otherwise, R" is selected from the group consisting of hydro, alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon atom) and heterocyclyl (bonded through a ring carbon atom), wherein said hydro, alkyl, cycloalkyl, aryl, heteroaryl and heterocyclyl are as defined herein.
As used herein, the term "aldehyde" group refers to a carbonyl group as defined herein above, wherein R" is hydro.
As used herein, the term "cycloketone" group refers to a cycloalkyl group as defined herein below, wherein one of the carbon atoms which form the ring has an "=O" bonded to it, i.e., one of the ring carbon atoms of the cycloalkyl group is a -C(=O)- group.
As used herein, the term "thiocarbonyl" refers to a -C(=S)R" group, wherein R" is as defined herein above.
As used herein, the term "O-carboxy" refers to an -OC(=O)R" group, wherein R" is as defined herein above.
As used herein, the term "C-carboxy" refers to a -C(=O)OR" group, wherein R" is as defined herein above.
As used herein, the term "ester" refers to a C-carboxy group as defined herein above or a molecule comprising such group, wherein R" is as defined herein above and R" is not hydro.
As used herein, the term "C-carboxy salt" refers to a -C(=0)0"M+ group or a molecule comprising such group, wherein M+ is selected from the group consisting of lithium, sodium, magnesium, calcium, potassium, barium, iron, zinc and quaternary ammonium, and further wherein the " + " in M+ does not reflect the actual number of positive charges which depends on the respective ion.
As used herein, the term "acetyl" refers to a -C(=O)CH3 group.
As used herein, the term "carboxyalkyl" refers to a -(CH2)rC(=O)OR" group, wherein r is 1 to 6 and R" is as defined herein above.
As used herein, the term "carboxyalkyl salt" refers to a
-(CH2)rC(=O)O"M+ group or a molecule comprising such group, wherein r is 1 to 6 and M+ is selected from the group consisting of lithium, sodium, potassium, calcium, magnesium, barium, iron, zinc and quaternary ammonium, and further wherein the " + " in M+ does not reflect the actual number of positive charges which depends on the respective ion. As used herein, the term "carboxylic acid" refers to a C-carboxy group as defined herein above, wherein R" is hydro.
As used herein, the term "haloalkyl" refers to an alkyl group substituted with 1 to 6 halo groups, wherein the alkyl group and the halo groups are as defined herein above, and further wherein the halo groups are independently selected. In a more specific definition, haloalkyl is a -CX3 group, wherein each X independently is a halo group.
As used herein, the term "trihalomethanesulfonyl" refers to an -S(=O)2CX3 group, wherein each X is a halo group as defined herein above.
As used herein, the term "cyano" refers to a -C≡N group.
As used herein, the term "cyanato" refers to an -OCN group.
As used herein, the term "isocyanato" refers to an -NCO group.
As used herein, the term "thiocyanato" refers to an -SCN group.
As used herein, the term "isothiocyanato" refers to an -NCS group.
As used herein, the term "sulfinyl" refers to an -S(=O)R" group, wherein R" is as defined herein above.
As used herein, the term "sulfonyl" refers to an -S(=O)2R" group, wherein R" is as defined herein above.
As used herein, the term "sulfonamido" refers to an -S(=O)2NRi 7Rig group, wherein Ri 7 and Ri 8 are as defined herein below. Unless specified otherwise, Rn and Ri8 are independently chosen from hydro, alkyl, aryl, carbocyclyl, heterocyclyl, -(CH2)aryl, -(CH2)carbocyclyl, and (CH2)heterocyclyl, wherein hydro, alkyl, aryl, carbocyclyl and heterocyclyl are as defined herein.
As used herein, the term "trihalomethanesulfonamido" refers to an -N(Ri7)S(=O)2CX3 group, wherein X is a halo group as defined herein above and Rn is as defined as herein above.
As used herein, the term "O-carbamyl" refers to an
Figure imgf000090_0001
group, wherein Rn and Ri8 are as defined herein above.
As used herein, the term "N-carbamyl" refers to an -N(Ri7)C(=O)ORi8 group, wherein Rn and R]8 are as defined herein above.
As used herein, the term "O-thiocarbamyl" refers to an -OC(=S)NRn Ri8 group, wherein Rn and Ri8 are as defined herein above.
As used herein, the term "N-thiocarbamyl" refers to an -N(Rn)C(=S)ORi g group, wherein Rn and Ri8 are as defined herein above.
As used herein, the term "amino" refers to an -NH2 group.
As used herein, the term "alkylamino" refers to an -NR23R24 group, wherein R23 and R24 are independently chosen from -H, Ci-8 alkyl (i.e., an alkyl having 1 to 8 carbon atoms), and phenyl.
As used herein, the term "C-amido" refers to a -C(=0)NRnRi8 group, wherein Rn and Ri8 are as defined herein above.
As used herein, the term "N-amido" refers to an -N(Rn)C(=0)Ri8 group, wherein Rn and Ri8 are as defined herein above. As used herein, the term "nitro" refers to an -NO2 group.
As used herein, the term "quaternary ammonium" refers to an -NR2oR2]R-22 group, wherein R2o, R21, and R22 are independently selected from the group consisting of hydro and Cj-6 alkyl.
As used herein, the term "methylenedioxy" refers to an -OCH2O- group, wherein the two oxygen atoms are bonded to adjacent ring carbon atoms.
As used herein, the term "ethylenedioxy" refers to an -OCH2CH2O- group, wherein the two oxygen atoms are bonded to adjacent ring carbon atoms.
As used herein, the term "carbocyclyl" refers to an all-carbon monocyclic or fused ring (i.e., rings which share an adjacent pair of ring carbon atoms) group, wherein one or more of the rings does not have a completely conjugated pi-electron system. In a more specific definition, it refers to a cycloalkyl group having 3 to 12 carbon atoms or a cycloalkenyl group having 3 to 12 carbon atoms. In another more specific definition, it refers to a cycloalkyl group having 3 to 6 carbon atoms or a cycloalkenyl group having 3 to 6 carbon atoms. Examples, without limitation, of carbocyclyl groups are cycloalkyls such as cyclopropane, cyclobutane, cyclopentane, cyclohexane, adamantane, cycloheptane and cycloalkenes such as cycloheptatriene, cyclopentene, and cyclohexadiene.
As used herein, the term "cycloalkyl" refers to an all-carbon monocyclic or fused ring (i.e., rings which share an adjacent pair of ring carbon atoms) group, wherein said monocyclic or fused ring group does not have a double or triple bond. In a more specific definition, it refers to a C3.12 cycloalkyl group, i.e., an all-carbon monocyclic or fused ring group having 3 to 12 carbon atoms, wherein said monocyclic or fused ring group does not have a double or triple bond. In another more specific definition, it refers to a C3-6 cycloalkyl group, i.e., an all-carbon monocyclic or fused ring group having 3 to 6 carbon atoms, wherein said monocyclic or fused ring group does not have a double or triple bond. Examples, without limitation, of cycloalkyl groups are cyclopropane, cyclobutane, cyclopentane, cyclohexane, adamantane, and cycloheptane.
As used herein, the term "heterocyclyl" (or "heterocycle" or "heterocyclic") refers to a saturated or partially saturated monocyclic or fused-ring polycyclic group having 3 to 14 ring atoms, said ring atoms comprising carbon atoms and from one to four heteroatoms independently selected from the group consisting of O, N, and S, wherein the nitrogen and sulfur heteroatoms can be optionally oxidized, the nitrogen heteroatoms can be optionally quaternized, and including any bicyclic group in which any of the above-defined heterocyclic rings is fused to a benzene ring. "Heterocyclyl" thus also include heteroaryl groups as defined herein below. In a more specific definition, "heterocyclyl" refers to a saturated or partially saturated 3- 7 membered monocyclic, or 7-10 membered bicyclic ring system, which consists of carbon atoms and from one to four heteroatoms independently selected from the group consisting of O, N, and S, wherein the nitrogen and sulfur heteroatoms can be optionally oxidized, the nitrogen heteroatoms can be optionally quaternized, and including any bicyclic group in which any of the above-defined heterocyclic rings is fused to a benzene ring. Non-limiting examples of saturated or partially saturated heterocyclyl groups include tetrahydrofuranyl, pyranyl, piperidinyl, piperazinyl, pyrrolidinyl, imidazolidinyl, imidazolinyl, indolinyl, isoindolinyl, quinuclidinyl, morpholinyl, isochromanyl, chromanyl, pyrazolidinyl, pyrazolinyl, tetronoyl and tetramoyl groups. Examples of "heterocycles" or "heterocyclic" rings also include, but are not limited to, morpholino, piperidyl, piperazinyl, pyrrolidinyl, thiomorpholino, homopiperazinyl, imidazolyl, imidazolidinyl, pyrazolidinyl, dioxanyl and dioxolanyl.
As used herein, the term "aryl" refers to an all-carbon monocyclic or fused-ring polycyclic (i.e., rings which share an adjacent pair of ring carbon atoms) aromatic group having a completely conjugated pi-electron system. Examples, without limitation, of aryl groups are phenyl, naphthalenyl and anthracenyl.
As used herein, the term "heteroaryl" refers to a monocyclic or fused-ring polycyclic group having 5 to 14 ring atoms; having 6, 10 or 14 pi electrons shared in a cyclic array; and containing carbon atoms and 1, 2 or 3 heteroatoms independently selected from the group consisting of O, N, and S. In a more specific definition, it refers to a monocyclic or fused-ring polycyclic aromatic group having 5 to 9 ring atoms and comprising 1 , 2 or 3 heteroatoms independently selected from the group consisting of O, N, and S. Non-limiting examples of heteroaryl groups include thienyl (thiophenyl), benzo[b]thienyl, naphtho[2,3-b]thienyl, thianthrenyl, furyl (furanyl), isobenzofuranyl, chromenyl, xanthenyl, phenoxanthiinyl, pyrrolyl (including, without limitation, 2H-pyrrolyl), imidazolyl, pyrazolyl, pyridyl (pyridinyl; including, without limitation, 2 -pyridyl, 3 -pyridyl, and 4-pyridyl), pyrazinyl, pyrimidinyl, pyridazinyl, indolizinyl, isoindolyl, indolyl (including, without limitation, 3H-indolyl), indazolyl, purinyl, 4H-quinolizinyl, isoquinolyl, quinolyl, phthalzinyl, naphthyridinyl, quinozalinyl, cinnolinyl, pteridinyl, carbazolyl, beta- carbolinyl, phenanthridinyl, acrindinyl, perimidinyl, phenanthrolinyl, phenazinyl, isothiazolyl, phenothiazinyl, isoxazolyl, furazanyl, phenoxazinyl, 1 ,4- dihydroquinoxaline-2,3-dione, 7-aminoisocoumarin, pyrido[l,2-a]pyrimidin-4-one, pyrazolo[l,5-a]pyrimidinyl (including, without limitation, pyrazolo[l,5-a]pyrimidin- 3-yl), l,2-benzoisoxazol-3-yl, benzimidazolyl, 2-oxindolyl and 2-oxobenzimidazolyl. When the heteroaryl group contains a nitrogen ring atom, such nitrogen atom may optionally be in the form of an N-oxide, e.g., a pyridyl N-oxide, pyrazinyl N-oxide and pyrimidinyl N-oxide.
As used herein and unless specified otherwise, the term "optional substituent" or "optionally substituted" refers to one or more substituents covalently linked to the parent group, wherein said substituents are independently chosen from halo, alkyl, alkynyl, alkenyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-carbocyclyl, -L- aryl, -L-heteroaryl, -L-heterocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido, wherein each L is independently chosen from -(CH2)n-(CH2)n-, -(CH2)nNH(CH2)n-, -(CH2)n0(CH2)n-, and - (CHa)nS(CH2)H-, and wherein each n is independently chosen from 0, 1 , 2, and 3. In an alternative definition, the one or more optional substituents are chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, - NH(Ci-3 alkyl), -NHC(K))(Ci-3 alkyl), -C(O)OH, -C(=O)O(C1-6 alkyl), -C(=0)(d-3 alkyl), -C(=0)NH2, -C(=O)NH(Ci-3 alkyl), -C(=O)NH(cycloalkyl), -C(=O)N(d-3 alkyl)2, -S(=O)2(Ci-3 alkyl), -S(=O)2NH2, -S(=O)2N(Ci -3 alkyl)2, -S(=O)2NH(Ci-3 alkyl), -CHF2, -OCF3, -OCHF2, -CF3, -CN, -NH2, -NO2, and tetrazolyl.
As used herein, the term "arylalkyl" refers to a CM O alkyl group (an alkyl group having 1 -10 carbon atoms), as defined herein above, substituted by a C6-I4 aryl group (an aryl group having 6 to 14 carbon atoms), as defined herein above. Non-limiting examples of arylalkyl groups include benzyl, phenethyl, and naphthylmethyl.
As used herein, the term "arylalkenyl" refers to a C2. io alkenyl group substituted by a C6-14 aryl group (an aryl group having 6 to 14 carbon atoms), as defined herein above.
As used herein, the term "arylalkynyl" refers to a C2.10 alkynyl group substituted by a C6-I4 aryl group (an aryl group having 6 to 14 carbon atoms), as defined herein above.
As used herein, the term "arylalkoxy" refers to a Ci-] 0 alkoxy group, as defined herein above, substituted by an aryl group, as defined herein above. Examples of arylalkoxy groups include benzyloxy and phenethyloxy.
As used herein, the term "aryloxy" refers to an oxygen substituted by a C6.14 aryl group, as defined herein above. Examples of aryloxy groups include phenoxy and A- methylphenoxy.
As used herein, the term "arylthio" refers to an -S-aryl group, wherein the aryl group is as defined herein above.
As used herein, the term "heteroarylthio" refers to an -S-heteroaryl group, wherein the heteroaryl group is as defined herein above. As used herein, the term "haloalkoxy" refers to an alkoxy group which is substituted with 1 to 6 halo groups, wherein the alkoxy group and the halo groups are as defined herein above, and further wherein the halo groups are independently selected.
As used herein, the term "haloaryl" refers to an aryl group which is substituted with 1 to 6 halo groups, wherein the aryl group and the halo groups are as defined herein above, and further wherein the halo groups are independently selected.
As used herein, the term "acylamino" refers to an -N(R17)C(^O)R18 group, wherein Rn and Ri8 are as defined herein above.
As used herein, the term "acyloxy" refers to an -O-C(=O)R17 group, wherein Ri7 is as defined herein above.
As used herein, the term "heteroaryloxy" refers to an -O-heteroaryl group, wherein the heteroaryl group is as defined herein above.
As used herein, the term "heteroarylalkoxy" refers to a Ci-10 alkoxy group, as defined herein above, substituted by a heteroaryl group, as defined herein above.
As used herein, the term "preventing an increase in a symptom" refers to both not allowing a symptom to increase or worsen, as well as reducing the rate of increase in the symptom. For example, a symptom can be measured as the amount of particular disease marker, i.e., a protein. In another example the symptom can be cognitive decline. Preventing an increase, according to the definition provided herein, means that the amount of symptom {e.g., protein or cognitive decline) does not increase or that the rate at which it increases is reduced.
As used herein, the term "treating a disease or disorder" refers to a slowing of or a reversal of the progress of the disease. Treating a disease or disorder includes treating a symptom and/or reducing the symptoms of the disease. As used herein, the term "preventing a disease or disorder" refers to a slowing of the disease or of the onset of the disease or the symptoms thereof. Preventing a disease or disorder can include stopping the onset of the disease or symptoms thereof. As used herein, the term "unit dosage form" refers to a physically discrete unit, such as a capsule or tablet suitable as a unitary dosage for a human patient. Each unit contains a predetermined quantity of a compound of Formula I, which was discovered or believed to produce the desired pharmacokinetic profile which yields the desired therapeutic effect. The dosage unit is composed of a compound of Formula I in association with at least one pharmaceutically acceptable carrier, salt, excipient, or combination thereof.
The patient or subject, such as the subject in need of treatment or prevention, may be e.g. a eukaryote, an animal, a vertebrate animal, a mammal, a rodent (e.g. a guinea pig, a hamster, a rat, a mouse), a murine (e.g. a mouse), a canine (e.g. a dog), a feline (e.g. a cat), an equine (e.g. a horse), a primate, a simian (e.g. a monkey or ape), a monkey (e.g. a marmoset, a baboon), an ape (e. g. gorilla, chimpanzee, orangutang, gibbon), or a human. The meaning of the terms "eukaryote", "animal", "mammal", etc. is well known in the art and can, for example, be deduced from Wehner und Gehring (1995; Thieme Verlag). In the context of this invention, it is particularly envisaged that animals are to be treated which are economically, agronomically or scientifically important. Scientifically important organisms include, but are not limited to, mice, rats, rabbits, fruit flies like Drosophila melagonaster and nematodes like Caenorhabditis elegans. Non-limiting examples of agronomically important animals are sheep, cattle and pig, while, for example, cats and dogs may be considered as economically important animals. Preferably, the subject/patient is a mammal; more preferably, the subject/patient is a human.
As used herein, the term "dose" or "dosage" refers the amount of active ingredient that an individual takes or is administered at one time. For example, a 40 mg dose of a compound of Formula I refers to, in the case of a twice-daily dosage regimen, a situation where the individual takes 40 mg of a compound of Formula I twice a day, e.g., 40 mg in the morning and 40 mg in the evening. The 40 mg of a compound of Formula I dose can be divided into two or more dosage units, e.g., two 20 mg dosage units of a compound of Formula I in tablet form or two 20 mg dosage units of a compound of Formula I in capsule form.
As used herein, a "pharmaceutically acceptable prodrug" is a compound that may be converted under physiological conditions or by solvolysis to the specified compound or to a pharmaceutically acceptable salt of such compound.
As used herein, a "pharmaceutically active metabolite" is intended to mean a pharmacologically active product produced through metabolism in the body of a specified compound or salt thereof. Metabolites of a compound may be identified using routine techniques known in the art and their activities determined using tests such as those described herein.
As used herein, a "pharmaceutically acceptable salt" is intended to mean a salt that retains the biological effectiveness of the free acids and bases of the specified compound and that is not biologically or otherwise undesirable. A compound for use in the invention may possess a sufficiently acidic, a sufficiently basic, or both functional groups, and accordingly react with any of a number of inorganic or organic bases, and inorganic and organic acids, to form a pharmaceutically acceptable salt. Exemplary pharmaceutically acceptable salts include those salts prepared by reaction of the compounds of the present invention with a mineral or organic acid or an inorganic base, such as salts including sulfates, pyrosulfates, bisulfates, sulfites, bisulfites, phosphates, monohydrophosphates, dihydrophosphates, metaphosphates, pyrophosphates, chlorides, bromides, iodides, acetates, propionates, decanoates, caprylates, acrylates, formates, isobutyrates, caproates, heptanoates, propiolates, oxalates, malonates, succinates, suberates, sebacates, fumarates, maleates, butyne-1 ,4 dioates, hexyne-l,6-dioates, benzoates, chlorobenzoates, methylbenzoates, dinitrobenzoates, hydroxybenzoates, methoxybenzoates, phthalates, sulfonates, xylenesulfonates, phenylacetates, phenylpropionates, phenylbutyrates, citrates, lactates, gamma-hydroxybutyrates, glycollates, tartrates, methane-sulfonates, propanesulfonates, naphthalene- 1 - sulfonates, naphthalene-2-sulfonates, and mandelates. As used herein, a "pharmaceutically acceptable carrier" refers to a non-API (API refers to Active Pharmaceutical Ingredient) substances such as disintegrators, binders, fillers, and lubricants used in formulating pharmaceutical products. They are generally safe for administering to humans according to established governmental standards, including those promulgated by the United States Food and Drug Administration and the European Medical Agency.
As is understood by the skilled artisan, certain variables in the list of substituents are repetitive (different name for the same substituent), generic to other terms in the list, and/or partially overlap in content with other terms. In the compounds of the invention, the skilled artisan recognizes that substituents may be attached to the remainder of the molecule via a number of positions and the preferred positions are as illustrated in the Examples.
Additionally, the compounds of Formula I can contain asymmetric carbon atoms and can therefore exist in racemic and optically active forms. Thus, optical isomers or enantiomers, racemates, tautomers, and diastereomers are also encompassed in the compounds of Formula I in all embodiments described herein. The methods of the present invention include the use of all such isomers and mixtures thereof. Methods of separation of enantiomeric and diastereomeric mixtures are well known to one skilled in the art. The present invention encompasses any isolated racemic or optically active form of compounds described in Formula I, or any mixture thereof. In one aspect, the compounds of the invention have a trans configuration around the cyclopropyl ring as in trans-phenylcyclopropylamine. In one aspect, the compounds of the invention have a cis configuration around the cyclopropyl ring as in cis- phenylcyclopropylamine.
Typically, compounds according to Formula I can be effective at an amount of from about 0.01 μg/kg to about 100 mg/kg per day based on total body weight. The active ingredient may be administered at once, or may be divided into a number of smaller doses to be administered at predetermined intervals of time. The suitable dosage unit for each administration can be, e.g., from about 1 μg to about 2000 mg, preferably from about 5 μg to about 1000 mg. It should be understood that the dosage ranges set forth above are exemplary only and are not intended to limit the scope of this invention. The therapeutically effective amount for each active compound can vary with factors including but not limited to the activity of the compound used, stability of the active compound in the patient's body, the severity of the conditions to be alleviated, the total weight of the patient treated, the route of administration, the ease of absorption, distribution, and excretion of the active compound by the body, the age and sensitivity of the patient to be treated, and the like, as will be apparent to a skilled artisan. The amount of administration can be adjusted as the various factors change over time.
For oral delivery, the active compounds can be incorporated into a formulation that includes pharmaceutically acceptable carriers such as binders (e.g., gelatin, cellulose, gum tragacanth), excipients (e.g., starch, lactose), lubricants (e.g., magnesium stearate, silicon dioxide), disintegrating agents (e.g., alginate, Primogel, and corn starch), and sweetening or flavoring agents (e.g., glucose, sucrose, saccharin, methyl salicylate, and peppermint). The formulation can be orally delivered in the form of enclosed gelatin capsules or compressed tablets. Capsules and tablets can be prepared in any conventional techniques. The capsules and tablets can also be coated with various coatings known in the art to modify the flavors, tastes, colors, and shapes of the capsules and tablets. In addition, liquid carriers such as fatty oil can also be included in capsules.
Suitable oral formulations can also be in the form of suspension, syrup, chewing gum, wafer, elixir, and the like. If desired, conventional agents for modifying flavors, tastes, colors, and shapes of the special forms can also be included. In addition, for convenient administration by enteral feeding tube in patients unable to swallow, the active compounds can be dissolved in an acceptable lipophilic vegetable oil vehicle such as olive oil, corn oil and safflower oil.
The active compounds can also be administered parenterally in the form of solution or suspension, or in lyophilized form capable of conversion into a solution or suspension form before use. In such formulations, diluents or pharmaceutically acceptable carriers such as sterile water and physiological saline buffer can be used. Other conventional solvents, pH buffers, stabilizers, anti-bacteria agents, surfactants, and antioxidants can all be included. For example, useful components include sodium chloride, acetates, citrates or phosphates buffers, glycerin, dextrose, fixed oils, methyl parabens, polyethylene glycol, propylene glycol, sodium bisulfate, benzyl alcohol, ascorbic acid, and the like. The parenteral formulations can be stored in any conventional containers such as vials and ampoules.
Routes of topical administration include nasal, bucal, mucosal, rectal, or vaginal applications. For topical administration, the active compounds can be formulated into lotions, creams, ointments, gels, powders, pastes, sprays, suspensions, drops and aerosols. Thus, one or more thickening agents, humectants, and stabilizing agents can be included in the formulations. Examples of such agents include, but are not limited to, polyethylene glycol, sorbitol, xanthan gum, petrolatum, beeswax, or mineral oil, lanolin, squalene, and the like. A special form of topical administration is delivery by a transdermal patch. Methods for preparing transdermal patches are disclosed, e.g., in Brown, et al. (1988) Ann. Rev. Med. 39:221 -229 which is incorporated herein by reference.
Subcutaneous implantation for sustained release of the active compounds may also be a suitable route of administration. This entails surgical procedures for implanting an active compound in any suitable formulation into a subcutaneous space, e.g., beneath the anterior abdominal wall. See, e.g., Wilson et al. (1984) J. Clin. Psych. 45:242-247. Hydrogels can be used as a carrier for the sustained release of the active compounds. Hydrogels are generally known in the art. They are typically made by crosslinking high molecular weight biocompatible polymers into a network, which swells in water to form a gel like material. Preferably, hydrogels are biodegradable or biosorbable. For purposes of this invention, hydrogels made of polyethylene glycols, collagen, or poly(glycolic-co-L-lactic acid) may be useful. See, e.g., Phillips et al. (1984) J. Pharmaceut. ScL, 73 : 1718-1720.
The active compounds can also be conjugated, to a water soluble non- immunogenic non-peptidic high molecular weight polymer to form a polymer conjugate. For example, an active compound is covalently linked to polyethylene glycol to form a conjugate. Typically, such a conjugate exhibits improved solubility, stability, and reduced toxicity and immunogenicity. Thus, when administered to a patient, the active compound in the conjugate can have a longer half-life in the body, and exhibit better efficacy. See generally, Burnham (1994) Am. J. Hosp. Pharm. 15 :210-218. PEGylated proteins are currently being used in protein replacement therapies and for other therapeutic uses. For example, PEGylated interferon (PEG-INTRON A®) is clinically used for treating Hepatitis B. PEGylated adenosine deaminase (ADAGEN®) is being used to treat severe combined immunodeficiency disease (SCIDS). PEGylated L-asparaginase (ONCAPSPAR®) is being used to treat acute lymphoblastic leukemia (ALL). It is preferred that the covalent linkage between the polymer and the active compound and/or the polymer itself is hydrolytically degradable under physiological conditions. Such conjugates known as "prodrugs" can readily release the active compound inside the body. Controlled release of an active compound can also be achieved by incorporating the active ingredient into microcapsules, nanocapsules, or hydrogels generally known in the art. Other pharmaceutically acceptable prodrugs of the compounds of this invention include, but are not limited to, esters, carbonates, thiocarbonates, N-acyl derivatives, N- acyloxyalkyl derivatives, quaternary derivatives of tertiary amines, N-Mannich bases, Schiff bases, aminoacid conjugates, phosphate esters, metal salts and sulfonate esters.
Liposomes can also be used as carriers for the active compounds of the present invention. Liposomes are micelles made of various lipids such as cholesterol, phospholipids, fatty acids, and derivatives thereof. Various modified lipids can also be used. Liposomes can reduce the toxicity of the active compounds, and increase their stability. Methods for preparing liposomal suspensions containing active ingredients therein are generally known in the art. See, e.g., U.S. Patent No. 4,522,81 1 ; Prescott, Ed., Methods in Cell Biology, Volume XIV, Academic Press, New York, N. Y. (1976).
The active compounds can also be administered in combination with another active agent that synergistically treats or prevents the same symptoms or is effective for another disease or symptom in the patient treated so long as the other active agent does not interfere with or adversely affect the effects of the active compounds of this invention. Such other active agents include but are not limited to anti-inflammation agents, antiviral agents, antibiotics, antifungal agents, antithrombotic agents, cardiovascular drugs, cholesterol lowering agents, anti-cancer drugs, hypertension drugs, and the like.
Examples of antineoplastic agents that can be used in combination with the compounds and methods of the present invention include, in general, and as appropriate, alkylating agents, anti-metabolites, epidophyllotoxins, antineoplastic enzymes, topoisomerase inhibitors, procarbazines, mitoxantrones, platinum coordination complexes, biological response modifiers and growth inhibitors, hormonal/anti-hormonal therapeutic agents and haematopoietic growth factors. Exemplary classes of antineoplastic include the anthracyclines, vinca drugs, mitomycins, bleomycins, cytotoxic nucleosides, epotbilones, discodermolides, pteridines, diynenes and podophyllotoxins. Particularly useful members of those classes include, for example, carminomycin, daunorubicin, aminopterin, methotrexate, methopterin, dichloromethotrexate, mitomycin C, porfiromycin, 5- fluorouracil, 6-mercaptopurine, gemcitabine, cytosine arabinoside, podophyllotoxin or podo-phyllotoxin derivatives such as etoposide, etoposide phosphate or teniposide, melphalan, vinblastine, vincristine, leurosidine, vindesine, leurosine, paclitaxel and the like. Other useful antineoplastic agents include estramustine, carboplatin, cyclophosphamide, bleomycin, gemcitibine, ifosamide, melphalan, hexamethyl melamine, thiotepa, cytarabin, idatrexate, trimetrexate, dacarbazine, L- asparaginase, camptothecin, CPT-I l , topotecan, ara-C, bicalutamide, flutamide, leuprolide, pyridobenzoindole derivatives, interferons and interleukins.
General Synthetic Route Description
The compounds of Formula (I), in which Rl , R2, R3, R4, R5, R6 and R7 represent a hydrogen atom and R8 represents an optionally substituted aryl, heteroaryl, or heterocyclic group can be synthesized by the general route described in the scheme
1.
Figure imgf000103_0001
(H) (IV)
Scheme 1
Commercially available trans-Phenylcyclopropylamine (ALDRICH) of Formula (II) is reductively akyiated using commercially available aldehydes of Formula (III) R8CHO, in which R8 is as defined earlier (e.g., in the markush groups described above), to give the compounds of Formula (IV), which are a particular case of the compounds claimed in the present invention.
The procedures for preparing the compounds of Formula (I), e.g., where the phenyl ring of the phenylcyclopropylamine core is substituted (in this scheme with a benzyloxy moiety; other moieities substituting the phenyl ring of the phenylcyclopropylamine core can be used as is recognized by the skilled artisan) are exemplified in Scheme 2.
Figure imgf000103_0002
Scheme 2
Cyclopropanation reaction of commercially available nitroolefin derivatives of Formula (V) using trimethylsulfoxonium iodide gives derivatives of Formula (VI). Reduction of the nitro group by zinc, followed by protection, with Boc, of the resulting amino group by tertbutyloxycarbonyl leads to the derivatives of Formula (VII). These derivatives were alkylated using a base and an alkylating reagent of Formula (VIII), where R8 is as defined before and X represents a halogen atom (e.g., -Cl or -Br). Deprotection of the Boc group using sulfuric acid affords the derivatives of Formula (IX), which are specific examples of the compounds of Formula (I).
Compounds of Formula (I), in which Rl , R2, R3, R4, R5 and R6 represent a hydrogen atom, R7 represents alkyl or cycloalkyl and R8 represents an optionally substituted aryl, heteroaryl, or heterocyclic group can be synthesized by the general route described in the scheme 3.
Figure imgf000104_0001
(!I) (Xl) Scheme 3
Commercially available trans-phenylcyclopropylamine (ALDRICH) of Formula (II) is reductively alkylated using commercially available ketones of Formula (X), in which R7 and R8 are as defined earlier, to give the compounds of Formula (XI), which are also subjects of the present invention.
Compounds of Formula (I), where Rl to R5 may be different than a hydrogen and R8 represents an optionally substituted oxadiazole can be synthesized by the general route described in the scheme 4. O
Br-
OMe
O, 2h
Figure imgf000105_0001
Scheme 4: ACN (acetonitπle), DIGLYME (bis(2-methoxyethyl) ether), DIPEA (N5N-Dn sopropylethylamme), EDC (l-ethyl-3-(3-dimethylaminopropyl) carbodiimide), THF (Tetrahydrofurane)
The trans phenylcyclopropylammo derivatives of formula (XIV) (including trans ((1S, 2R), (IR, 2S)) version as well the individual diastereoisomers corresponding to (IS, 2R) and (IR, 2S) can be used) are reacted with methyl 2-bromoacetate in acetonitπle usmg N,N-dnsopropylethylamme as a base to give methyl 2-((trans)-2- phenylcyclopropylamino)acetate derivatives of formula (XVI) Hydrolysis with lithium hydroxide using tetrahydrofurane-water solution as a solvent and later reaction with t-butyl dicarbonate in tetrahydrofurane leads to 2-(tert- butoxycarbonyl((trans)-2-phenylcyclopropy])amino)acetic acid derivatives of formula (XVII), which are reacted with commercially available formamidoxime derivatives of formula (XVIII) and l-ethyl-3-(3-dimethylammopropyl)carbodnmide in diglyme to obtain tert-butyl (l ,2,4-oxadiazol-5-yl)methyl((trans)-2- phenylcyclopropyl)carbamate derivatives of formula (XIX). Final Boc-deprotection using HCl 2M in diethyl ether using diethyl ether as a solvent leads to the formation of the corresponding hydrochloride salt of the (trans)-N-((l ,2,4-oxadiazol-5- yl)methyl)-2-phenylcyclopropanamine derivatives of formula (XX), which are also subjects of the present invention.
The compounds in the examples below can be synthesized using these procedures described above or modifications thereof by an ordinary artisan skilled in the art of synthetic organic chemistry.
It is to be understood that wherever a substituent in a structure depicted herein is not specified (e.g. is "missing" in the structure) said substituent is a hydrogen atom.
The title Example compounds below were named using the ChemBioDraw Ultra version 1 1.0.1 by CambridgeSoft program. In the case of a conflict between a name and a drawn structure, the drawn structure is the controlling definition.
Examples
Example 1 : (trans)-N-(4-fluorobenzyl)-2-phenylcyclopropanamine
Figure imgf000106_0001
A mixture of (trans)-2-phenylcyclopropanamine hydrochloride (0.25 g, 1.50 mmol) and 4-fluorobenzaldehyde (0.18 mL, 1.65 mmol) in CH2Cl2 (8 mL) and water (0.5 mL) was vigorously stirred at room temperature for 10 min. Then, NaBH(OAc)3 (0.41 g, 1.95 mmol) was slowly added and stirring continued for 1.5 h. The reaction mixture was washed with an aqueous saturated solution of NaHCO3 (10 mL), the organic layer was dried over anhydrous Na2SO4, filtered and, after removal of the solvent, the residual oil was purified by column chromatography on silica gel (3% MeOH/CH2Cl2) affording 0.18 g of N-(4-fluorobenzyl)-N-[(trans)-2- phenylcyclopropyl]amine [R/= 0.5 (5% MeOHVCH2Cl2), colorless oil, 49% yield].
Example 2: (trans)-N-(4-fluorobenzyl)-2-phenylcyclopropanaminium chloride
Figure imgf000107_0001
2 M ethereal solution of HCl (0.24 mL, 0.47 mmol) was slowly added to a solution of N-(4-fluorobenzyl)-N-[(trans)-2-phenylcyclopropyl]amine (95 mg, 0.39 mmol) in dry Et2O (10 mL) cooled at -78 0C, and allowed to reach room temperature. After 10 min the white precipitate was allowed to settle and Et2O was decanted, the solid was washed with Et2O (10 mL) and vacuum dried, rendering 90 mg of N-(4- fluorobenzyl)-N-[(trans)-2-phenylcyclopropyl]amine hydrochloride (white solid, 83% yield).
1H-NMR (MeOD, 250 MHz, δ): 7.59-7.49 (m, 2H, ArH); 7.34-7.05 (m, 7H, ArH); 4.38 (s, 2H, CH2); 3.02-2.93 (m, I H, CH); 2.52-2.40 (m, IH, CH); 1.58-1.31 (m, 2H, CH2).
Example 3 : 4-(((trans)-2-phenylcyclopropylamino)methyl)benzonitrile
Figure imgf000107_0002
A mixture of (trans)-2-phenylcyclopropanamine hydrochloride (0.25 g, 1.50 mmol) and 4-cyanobenzaldehyde (0.22 g, 1.65 mmol) in CH2Cl2 (8 mL) and water (0.5 mL) was vigorously stirred at room temperature for 10 min. Then, NaBH(OAc)3 (0.41 g, 1.95 mmol) was slowly added and stirring continued for 1.5 h. The reaction mixture was washed with an aqueous saturated solution Of NaHCO3 (10 mL), the organic layer was dried over anhydrous Na2SO4, filtered and, after removal of the solvent, the residual oil was purified by column chromatography on silica gel (1% MeOH/CH2Cl2) affording 0.07 g of 4-({ [(trans)-2- phenylcyclopropyl]amino}methyl)benzonitrile [R/= 0.6 (5% MeOHZCH2CIi), colorless oil, 19% yield].
Example 4: (trans)-N-(4-cyanobenzyl)-2-phenylcyclopropanaminium chloride
Figure imgf000108_0001
2 M ethereal solution of HCl (0.14 mL, 0.27 mmol) was slowly added to a solution of 4-({ [(trans)-2-phenylcyclopropyl]amino}methyl)benzonitrile (67 mg, 0.27 mmol) in dry Et2O (5 mL) cooled at -78 0C, and allowed to reach room temperature. After 10 min the white precipitate was allowed to settle and Et2O was decanted, the solid was washed with Et2O (20 mL) and vacuum dried, rendering 65 mg of 4-({ [(trans)- 2-phenylcyclopropyl]amino}methyl)benzonitrile hydrochloride (white solid, 84% yield).
1H-NMR (MeOD, 250 MHz, δ): 7.81 (d, J= 8.5 Hz, 2H, ArH); 7.71 (d, J= 8.5 Hz, 2H, ArH); 7.35-7.19 (m, 3H, ArH); 7.14-7.07 (m, 2H, ArH); 4.49 (s, 2H, CH2); 3.06-2.98 (m, IH, CH); 2.52-2.43 (m, IH, CH); 1.61-1.50 (m, I H, CH2); 1.44-1.33 (m, IH, CH2).
Example 5 : (trans)-2-phenyl-N-(4-(trifluoromethyl)benzyl)cyclopropanamine
Figure imgf000108_0002
A mixture of (trans)-2-phenylcyclopropanamine hydrochloride (0.23 g, 1.36 mmol) and 4-(trifluoromethyl)benzaldehyde (0.20 mL, 1.49 mmol) in CH2Cl2 (8 mL) and water (0.5 mL) was vigorously stirred at room temperature for 10 min. Then, NaBH(OAc)3 (0.37 g, 1.76 mmol) was slowly added and stirring continued for 30 min. The reaction mixture was washed with an aqueous saturated solution of NaHCO3 (8 mL), the organic layer was dried over anhydrous Na2SO4, filtered and, after removal of the solvent, the residual oil was purified by column chromatography on silica gel (1% MeOHZCH2Cl2) affording 0.15 g of N-[(trans)-2- phenylcyclopropyl]-N-[4-(trifluoromethyl)benzyl]amine [R/= 0-6 (5%
MeOH/CH2Cl2), colorless oil, 38% yield].
Example 6: (trans)-2-phenyl-N-(4-(trifluoromethyl)benzyl)cyclopropanaminium chloride
Figure imgf000109_0001
2 M ethereal solution of HCl (0.28 mL, 0.57 mmol) was slowly added to a solution of N-[(trans)-2-phenylcyclopropyl]-N-[4-(trifluoromethyl)benzyl]amine (150 mg, 0.51 mmol) in dry Et2O (8 mL) cooled at -78 0C, and allowed to reach room temperature. After 10 min the white precipitate was allowed to settle and Et2O was decanted, the solid was washed with Et2O (10 mL) and vacuum dried, rendering 105 mg of N-[(trans)-2-phenylcyclopropyl]-N-[4-(trifluoromethyl)benzyl]amine hydrochloride (white solid, 62% yield). 1H-NMR (MeOD, 250 MHz, δ): 7.78-7.65 (m, 4H, ArH); 7.34-7.20 (m, 3H, ArH); 7.13-7.06 (m, 2H, ArH); 4.49 (s, 2H, CH2); 3.07-2.97 (m, IH, CH); 2.51 -2.40 (m, IH, CH); 1.61 -1.49 (m, IH, CH2); 1.46-1.34 (m, I H, CH2).
Example 7 : (trans)-2-phenyl-N-(pyridin-2-ylmethyl)cyclopropanamine
Figure imgf000109_0002
A mixture of (trans)-2-phenylcyclopropanamine hydrochloride (0.25 g, 1.50 mmol) and pyridine-2-carbaldehyde (0.17 mL, 1.80 mmol) in CH2Cl2 (12 mL) and water (0.5 mL) was vigorously stirred at room temperature for 10 min. Then, NaBH(OAc)3 (0.48 g, 2.25 mmol) was slowly added and stirring continued for 2 h. The reaction mixture was washed with an aqueous saturated solution of NaHCO3 (10 rnL), the organic layer was dried over anhydrous Na2SO4, filtered and, after removal of the solvent, the residual oil was purified by column chromatography on silica gel [4% MeOH + 1% NH3 (aq)/CH2Cli] followed by preparative layer chromatography (5% MeOH/CH2Cl2) affording 0.08 g of N-[(trans)-2-phenylcyclopropyl]-N-(pyridin-2- ylmethyl)amine [R/= 0.3 (5% MeOH/CH2Cl2), colorless oil, 24% yield]. 1 H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 5H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44-2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01-1.92 (m, IH, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
The following examples 8 to 25 have been synthesized using the procedure described for examples 1 -6 starting with the corresponding aldehydes.
Example 8: (trans)-2-phenyl-N-(pyridin-3-ylmethyl)cyclopropanamine
Figure imgf000110_0001
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, I H, ArH); 7.28-7.09 (m, 5H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44-2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01 -1.92 (m, IH, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
Example 9: (trans)-2-phenyl-N-(pyridin-4-ylmethyl)cyclopropanamine
Figure imgf000110_0002
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 5H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44-2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01-1.92 (m, I H, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2). Example 10: (trans)-N-((6-methylpyridin-2-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000111_0001
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 5H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44-2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01-1.92 (m, 4H); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
Example 1 1 : (trans)-2-phenyl-N-(thiazol-2-ylmethyl)cyclopropanamine
Figure imgf000111_0002
1H-NMR (CDCl3, 250 MHz, δ): 7.72 (d, J= 3.3 Hz, IH, ArH); 7.28-7.09 (m, 4H, ArH); 7.04-6.96 (m, 2H, ArH); 4.24 (s, 2H, CH2); 2.54-2.46 (m, I H, CH); 2.37 (br s, IH, NH); 2.05-1.94 (m, IH, CH); 1.19-1.09 (m, IH, CH2); 1.05-0.95 (m, IH, CH2).
Example 12: (trans)-2-phenyl-N-(thiophen-2-ylmethyl)cyclopropanamine
Figure imgf000111_0003
1H-NMR (CDCl3, 250 MHz, δ): 7.72 (d, 2H, ArH); 7.28-7.09 (m, 4H, ArH); 7.04- 6.96 (m, 2H, ArH); 4.24 (s, 2H, CH2); 2.54-2.46 (m, IH, CH); 2.37 (br s, IH, NH); 2.05-1.94 (m, IH, CH); 1.19-1.09 (m, IH, CH2); 1.05-0.95 (m, IH, CH2).
Example 13 : (trans)-N-((3-bromothiophen-2-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000112_0001
1H-NMR (CDCl3, 250 MHz, δ): 7.72 (d, IH, ArH); 7.28-7.09 (m, 4H, ArH); 7.04- 6.96 (m, 2H, ArH); 4.24 (s, 2H, CH2); 2.54-2.46 (m, IH, CH); 2.37 (br s, I H, NH); 2.05-1.94 (m, IH, CH); 1.19-1.09 (m, I H, CH2); 1.05-0.95 (m, IH, CH2).
Example 14: (trans)-N-((4-bromothiophen-2-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000112_0002
1H-NMR (CDCl3, 250 MHz, δ): 7.72 (d, IH, ArH); 7.28-7.09 (m, 4H, ArH); 7.04- 6.96 (m, 2H, ArH); 4.24 (s, 2H, CH2); 2.54-2.46 (m, IH, CH); 2.37 (br s, IH, NH); 2.05-1.94 (m, IH, CH); 1.19-1.09 (m, IH, CH2); 1.05-0.95 (m, IH, CH2).
Example 15: (trans)-N-(3,4-dichlorobenzyl)-2-phenylcyclopropanamine
Figure imgf000112_0003
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 5H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44-2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01 -1.92 (m, IH, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
Example 16: (trans)-N-(3-fluorobenzyl)-2-phenylcyclopropanaminium chloride
Figure imgf000113_0001
1H-NMR (MeOD, 250 MHz, δ): 7.59-7.49 (m, 2H, ArH); 7.34-7.05 (m, 7H, ArH); 4.38 (s, 2H, CH2); 3.02-2.93 (m, IH, CH); 2.52-2.40 (m, IH, CH); 1.58-1.31 (m, 2H, CH2).
Example 17: (trans)-N-(2-fluorobenzyl)-2-phenylcyclopropanamine
Figure imgf000113_0002
1H-NMR (MeOD, 250 MHz, δ): 7.59-7.49 (m, 2H, ArH); 7.34-7.05 (m, 7H, ArH); 4.38 (s, 2H, CH2); 3.02-2.93 (m, IH, CH); 2.52-2.40 (m, IH, CH); 1.58-1.31 (m, 2H, CH2).
Example 18 : (trans)-2-phenyl-N-(quinolin-4-ylmethyl)cyclopropanamine
Figure imgf000113_0003
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 7H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44-2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01 -1.92 (m, IH, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
Example 19 : (trans)-N-(3 -methoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000114_0001
1H-NMR (MeOD, 250 MHz, δ): 7.56-7.42 (m, 5H, ArH); 7.36-7.19 (m, 3H, ArH); 7.16-7.09 (m, 2H, ArH); 4.38 (s, 2H, CH2); 3.03-2.95 (m, IH, CH); 2.50-2.39 (m, IH, CH); 1.55-1.33 (m, 2H, CH2).
Example 20: (trans)-2-phenyl-N-((6-(trifluoromethyl)pyridin-3- yl)methyl)cyclopropanamine
Figure imgf000114_0002
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44- 2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01-1.92 (m, IH, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
Example 21 : (trans)-N-((6-chloropyridin-3-yl)methyl)-2- phenylcyclopropanamine
Figure imgf000114_0003
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 4H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44-2.37 (m, IH, CH); 2.09 (br s, I H, NH); 2.01-1.92 (m, I H, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
Example 22: (trans)-N-((4-methylpyridin-2-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000115_0001
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 4H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 2H, CH2); 2.44-2.37 (m, IH, CH); 2.29 (s, 3H, CH3); 2.09 (br s, IH, NH); 2.01-1.92 (m, IH, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
Example 23 : (trans)-N-((6-methoxypyridin-2-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000115_0002
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 4H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 5H, CH2+CH3); 2.44-2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01-1.92 (m, IH, CH); 1.19-1.10 (m, I H, CH2); 1.02-0.94 (m, I H, CH2).
Example 24: 2-(((trans)-2-phenylcyclopropylamino)methyl)pyridin-3-ol
Figure imgf000115_0003
1H-NMR (CDCl3, 250 MHz, δ): 8.60-8.54 (m, IH, ArH); 7.62 (td, J= 7.8, 1.6 Hz, IH, ArH); 7.28-7.09 (m, 3H, ArH); 7.03-6.97 (m, 2H, ArH); 4.01 (s, 5H, CH2+CH3); 2.44-2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01-1.92 (m, IH, CH); 1.19-1.10 (m, IH, CH2); 1.02-0.94 (m, IH, CH2).
Example 25 : (trans)-N-((6-bromopyridin-2-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000116_0001
1H-NMR (CDCl3, 250 MHz, δ): 7.60-7.00 (m, 8H, ArH); 3.90 (s, 2H, CH2); 2.44- 2.37 (m, IH, CH); 2.09 (br s, IH, NH); 2.01 -1.92 (m, 4H); 1.19-1.10 (m, I H, CH2); 1.02-0.94 (m, IH, CH2).
Example 26: 4-(((trans)-2-(4-
(benzyloxy)phenyl)cyclopropylamino)methyl)benzonitrile
Figure imgf000116_0002
1H-NMR (MeOD, 250 MHz, δ): 7.88-7.22 (m, 9H, ArH); 6.82 (m, 4H, ArH); 5.01 (s, 2H, CH2); 3.85 (s, 2H, CH2); 2.25-2.18 (m, IH, CH); 1.90-1.81 (m, IH, CH); 1.07- 0.97 (m, IH, CH2); 0.95-0.85 (m, IH, CH2).
Example 27: (trans)-N-(4-(benzyloxy)benzyl)-2-phenylcyclopropanamine
Figure imgf000116_0003
1H-NMR (MeOD, 250 MHz, δ): 7.44-7.21 (m, 9H, ArH); 6.94-6.81 (m, 4H, ArH); 5.01 (s, 2H, CH2); 3.82 (s, 2H, CH2); 2.25-2.18 (m, IH, CH); 1.90-1.81 (m, IH, CH); 1.07-0.97 (m, IH, CH2); 0.95-0.85 (m, IH, CH2). Intermediate 1 : 1 -(benzyloxy)-4-[(trans)-2-nitrocyclopropyl]benzene
Figure imgf000117_0001
Trimethylsulfoxonium iodide (0.62 g, 2.82 mmol) was added in portions to a solution of r-BuOK (0.32 g, 2.82 mmol) in dry DMSO (5 mL). After 10 min a solution of l -(benzyloxy)-4-[(E)-2-nitrovinyl]benzene (0.60 g, 2.35 mmol) in DMSO (5 mL) was transferred via canula and the mixture was stirred at room temperature for 6 h. The reaction was poured over water (10 mL) and extracted with Et2O (3x10 mL); the organic layers were washed with brine (2x15 mL), dried over anhydrous Na2SO4 and filtered. After removal of the solvent, the residual orange oil was purified by column chromatography on silica gel (5% EtOAc/hexanes) affording 0.16 g of l -(benzyloxy)-4-[(lR,2S)-2-nitrocyclopropyl]benzene [R/= 0.5 (20% EtOAc/hexanes), white solid, 26% yield].
Intermediate 2: Trans-2-[4-(benzyloxy)phenyl]cyclopropanamine
Figure imgf000117_0002
Zn dust (1.97 g, 30 mol) was added in small portions, over a period of 30 min, to a vigorously stirred solution of l -(benzyloxy)-4-[(lR,2S)-2-nitrocyclopropyl]benzene (0.81 g, 3.0 mmol) in /-PrOH (25 mL) and HCl (1 1 mL of aqueous solution 2.7 N, 30 mmol). After 17 h the mixture was filtered through a pad of celite, that was washed with 10 mL of methanol. The filtrate was concentrated and 10 mL of water were added, washing with CH2Cl2 (3x15 mL). The organic layers were dried over anhydrous Na2SO4 and filtered. After removal of the solvent, the crude product was purified by column chromatography on silica gel (10% MeOHZCH2CIi) affording 0.50 g of (trans)-2-[4-(benzyloxy)phenyl]cyclopropanamine [R/= 0.2 (10% MeOH/CH2Cl2), white solid, 70% yield]. 1H-NMR (MeOH, 250 MHz, δ): 7.45-7.27 (m, 5H, ArH); 6.96 (d, J= 8.5 Hz, 2H, ArH); 6.86 (d, J= 8.5 Hz, 2H, ArH); 5.03 (s, 2H, CH2); 2.41 -2.34 (m, IH, CH); 1.86-1.76 (m, IH, CH); 0.98-0.85 (m, 2H, CH2).
Intermediate 3 : Intermediate tert-Butyl (trans)-2-[4-(benzyloxy)phenyl] cyclopropylcarbamate
Figure imgf000118_0001
200mg of intermediate x (trans)-2-[4-(benzyloxy)phenyl] cyclopropanamine were dissolved in pyridine (5mL) and tertbutyl carbonate (200 mg) was then added. The mixture was stirred at room temperature for 12h, the poured into ice-water. The desired compound was filtered and dried. 1H-NMR (MeOH, 250 MHz, δ): 7.45-7.27 (m, 5H, ArH); 6.96 (d, J= 8.5 Hz, 2H, ArH); 6.86 (d, J= 8.5 Hz, 2H, ArH); 5.03 (s, 2H, CH2); 2.41 -2.34 (m, IH, CH); 1.86-1.76 (m, 1OH, CH); 0.98-0.85 (m, 2H, CH2).
Example 28 : (trans)-N-benzyl-2-(4-(benzyloxy)phenyl)cyclopropanamine
Figure imgf000118_0002
NaH (17 mg, 60% in mineral oil, 0.42 mmol) was added to a solution of tert-Butyl (trans)-2-[4-(benzyloxy)phenyl]cyclopropylcarbamate (110 mg, 0.32 mmol) in DMF (4 rnL) at 0 0C. After 30 minutes, benzyl bromide (0.05 mL, 0.42 mmol) was added and the reaction was allowed to reach r.t. and stirred for additional 16 h. The reaction mixture was poured into H2O (15 mL) and extracted with Et2O (3x5 mL); the organic layers were washed with brine (10 mL), dried over anhydrous Na2SO4 and filtered.
After removal of the solvent, the residual yellowish oil was solved in a mixture of l ,4-dioxane/H2SO4 (4 mL, 10: 1 , v/v) and stirred for 40 min. The solution was basified by addition of 6 mL of aqueous NaOH (10%) and extracted with Et2O (2x10 niL); the organic layers were washed with brine (15 mL), dried over anhydrous Na2SO4 and filtered. After removal of the solvent, the crude residue was purified by column chromatography on silica gel (10-40% EtOAc/hexanes) to afford 58 mg of N-benzyl-N-{(trans)-2-[4-(benzyloxy)phenyl]cyclopropyl} amine [R/= 0.2 (5% MeOH/CH2Cl2), white solid, 55% yield]. 1H-NMR (MeOD, 250 MHz, δ): 7.44-7.21 (m, 1OH, ArH); 6.94-6.81 (m, 4H, ArH); 5.01 (s, 2H, CH2); 3.82 (s, 2H, CH2); 2.25- 2.18 (m, IH, CH); 1.90-1.81 (m, IH, CH); 1.07-0.97 (m, IH, CH2); 0.95-0.85 (m, IH, CH2).
Following examples have been synthesized using the procedure described for Example 28 and the corresponding starting materials.
Example 29: (trans)-2-(4-(benzyloxy)phenyl)-N-(4- methoxybenzyl)cyclopropanamine
Figure imgf000119_0001
1H-NMR (MeOD, 250 MHz, δ): 7.44-7.21 (m, 9H, ArH); 6.94-6.81 (m, 4H, ArH); 5.01 (s, 2H, CH2); 3.65 (s, 3H, OCH3); 3.82 (s, 2H, CH2); 2.25-2.18 (m, IH, CH); 1 .90-1.81 (m, IH5 CH); 1.07-0.97 (m, IH, CH2); 0.95-0.85 (m, IH, CH2).
Example 30: (trans)-2-(4-(benzyloxy)phenyl)-N-(4- fluorobenzyl)cyclopropanamine
Figure imgf000119_0002
1H-NMR (MeOD, 250 MHz, δ): 7.44-7.21 (m, 8H, ArH); 6.94-6.81 (m, 4H, ArH); 5.01 (s, 2H, CH2); 3.82 (s, 2H, CH2); 2.25-2.18 (m, IH, CH); 1 .90-1 .81 (m, I H, CH); 1.07-0.97 (m, IH, CH2); 0.95-0.85 (m, IH, CH2). Following examples have been synthesized using the procedure described for Examples 1 and 2 and the corresponding starting materials.
Example 31 : (trans)-2-phenyl-N-(quinolin-2-ylmethyl)cyclopropanamine
Figure imgf000120_0001
1H-NMR (CDC13) δ (ppm): 1.00 (q, IH), 1.18 (quin, IH), 2.00 (m, IH), 2.49 (m, IH), 4.22 (s, 2H), 7.02 (d, 2H), 7.16 (q, 2H), 7.23 (d, IH), 7.40 (d, IH), 7.53 (t, IH), 7.69 (t, IH), 7.80 (d, IH), 8.09 (t, 2H). MS (M+H) : 275.0
Example 32: (trans)-2-pheny]-N-((5-(trifluoromethyl)pyridin-2-yl)methyl) cyclopropanamine
Figure imgf000120_0002
1H-NMR (CDC13) δ (ppm): 0.98 (q, IH), 1.13 (quin, IH), 1.93 (m, IH), 2.40 (m, IH), 4.07 (s, 2H), 6.98 (d, 2H), 7.13 (d, IH), 7.22 (t, 2H), 7.40 (d, IH), 7.84 (d, IH), 8.82 (s, IH). MS (M+H) : 292.8
Example 33: (trans)-N-((3-fluoropyridin-2-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000120_0003
1H-NMR (CDC13) δ (ppm): 1.01 (q, IH), 1.18 (quin, IH), 1.99 (m, IH), 2.41 (m, IH), 4.11 (s, 2H), 7.02 (d, 2H), 7.25 (m, 5H), 8.38 (s, IH). MS (M+H) : 242.8
Example 34: (trans)-2-phenyl-N-(quinolin-3-ylmethyl)cyclopropan amine
Figure imgf000121_0001
1H-NMR (CDC13) δ (ppm): 1.00 (q, IH), 1.12 (quin, IH), 1.94 (m, IH), 2.40 (m, IH), 4.10 (s, 2H), 6.95 (d, 2H), 7.14 (t, IH), 7.20 (t, 2H), 7.54 (t, IH), 7.70 (t, IH), 7.76 (d, IH), 8.04 (s, IH), 8.10 (d, IH), 8.90 (s, IH). MS (M+H) : 275.0
Example 35: (trans)-N-((6-methoxypyridin-3-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000121_0002
1H-NMR (CDC13) δ (ppm): 0.98 (q, IH), 1.10 (quin, IH), 1.94 (m, IH), 2.36 (m, IH), 3.82 (s, 2H), 3.92 (s, 3H), 6.70 (d, IH), 7.00 (d, 2H), 7.14 (t, IH), 7.24 (d, 2H), 7.54 (d, IH), 8.08 (s, IH). MS (M+H) : 255.0
Example 36: (trans)-N-((5-methoxypyridin-3-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000121_0003
1H-NMR (CDC13) δ (ppm) 0 98 (q, IH), 1 12 (quin, IH), 1 92 (m, IH), 2 36 (m, IH), 3 78 (s, 2H), 3 90 (s, 3H), 6 98 (s, 2H), 7 14 (s, 2H), 7 24 (t, 2H), 8 20 (d, 2H) MS (M+H) 255 0
Example 37 (trans)-N-((2-methoxypyπdin-3-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000122_0001
1H-NMR (CDC13) δ (ppm) 0 98 (q, IH), 1 12 (quin, IH), 1 92 (m, IH), 2 32 (m, IH), 3 84 (s, 2H), 3 94 (s, 3H), 6 82 ^, IH), 7 00 (d, 2H), 7 i4 (t, IH), 7 24 (d, 2H), 7 48 (d, IH), 8 08 (d, IH) MS (M+H) 255 0
Example 38 (trans)-N-((3H-indol-3-yl)methyl)-2-phenylcyclopiopanamine
Figure imgf000122_0002
1H-NMR (CDC13) δ (ppm) 1 00 (q, IH), 1 20 (quin, IH), 2 02 (m, IH), 2 48 (m, IH), 4 10 (s, 2H), 7 02 (d, 2H), 7 14 (m, 4H), 7 20 (t, 2H), 7 36 (d, IH), 7 66 (d, IH), 8 02 (s, IH) MS (M+H) 263 0
Example 39 3-(((trans)-2-phenylcyclopropylamino)methyl)benzonitπle
Figure imgf000122_0003
1H-NMR (CDC13) δ (ppm): 1.00 (q, IH), 1.10 (qum, IH), 1.92 (m, IH), 2.36 (m, IH), 3.92 (s, 2H), 6.96 (d, 2H), 7.16 (t, IH), 7.26 (d, 2H), 7.40 (t, IH), 7.56 (d, 2H), 7.62 (s, IH). MS (M+H) : 249.0
Example 40: (trans)-N-(2-methoxybenzyl)-2-phenylcyclopropanamme
Figure imgf000123_0001
1H-NMR (CDC13) 5 (ppm): 0.96 (q, IH), 1.12 (qum, IH), 1.88 (m, IH), 2.36 (m, IH), 3.80 (s, 3H), 3.90 (s, 2H), 6.88 (q, 2H), 6.98 (d, 2H), 7.12 (d, IH), 7.21 (q, 4H). MS (M+H) : 257.1
Example 41 : 3-(((trans)-2-phenylcyclopropylamino)methyl)pyπdin-2-amme
Figure imgf000123_0002
1H-NMR (CDC13) δ (ppm): 1.01 (q, IH), 1.08 (qum, IH), 1.98 (m, IH), 2.34 (m, IH), 3.86 (s, 2H), 5.44 (br, 2H), 6.56 (t, IH), 6.92 (d, 2H), 7.14 (t, IH), 7.24 (t, 3H), 7.98 (d, IH). MS (M+H) : 240.0
Example 42: (trans)-N-((2-chloropyπdin-3-yl)methyl)-2-phenylcyclopropanamine
Figure imgf000123_0003
1H-NMR (CDC13) θ (ppm): 1.01 (q, IH), 1.16 (quin, IH), 1.98 (m, IH), 2.36 (m, IH), 3.99 (s, 2H), 6.99 (d, 2H), 7.18 (m, 2H), 7.26 (d, 2H), 7.72 (q, IH), 8.30 (d, IH). MS (M+H) : 259.0 // 260.9
Example 43: (trans)-N-(3,4-dimethoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000124_0001
1H-NMR (CDC13) δ (ppm): 0.98 (q, IH), 1.14 (quin, IH), 1.94 (m, IH), 2.36 (m, IH), 3.76 (s, 3H), 3.84 (s, 2H), 3.88 (s, 3H), 6.80 (m, 3H), 7.00 (d, 2H), 7.14 (t, IH), 7.23 (d, 2H). MS (M+H) : 284.1
Example 44: (trans)-N-((2,3-dihydrobenzofuran-5-yl)methyl)-2- phenylcyclopropanamine hydrochloride
Figure imgf000124_0002
1H-NMR (DMSO-d6) 6 (ppm): 1.24 (q, IH), 1.48 (quin, IH), 2.40 (m, IH), 2.84 (br, IH), 3.10 (q, 2H), 4.16 (s, 2H), 4.52 (t, 2H), 6.76 (d, IH), 7.09 (d, 2H), 7.22 (q, 2H), 7.28 (t, 2H), 7.34 (s, IH), 9.50 (br, 2H). MS (M+H) : 266.1
Example 45: (trans)-N-(benzo[d][l,3]dioxol-5-ylmethyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000124_0003
1H-NMR (DMSO-d6) δ (ppm): 1.24 (q, IH), 1.48 (quin, IH), 2.44 (m, IH), 2.82 (br, IH), 4.18 (s, 2H), 6.02 (s, 2H), 6.92 (d, IH), 7.09 (d, IH), 7.12 (t, 3H), 7.20 (t, IH), 7.27 (t, 2H), 9.60 (br, 2H). MS (M+H) : 268.1
Example 46: (trans)-N-((2,3-dihydrobenzo[b][l,4]dioxin-6-yl)methyl)-2- phenylcyclopropanamine hydrochloride
Figure imgf000125_0001
1H-NMR (DMSO-d6) δ (ppm): 1.24 (q, IH), 1.48 (quin, IH), 2.48 (br, IH), 4.12 (br, 2H), 4.20 (s, 4H), 6.78 (d, IH), 6.92 (d, IH), 7.09 (s, 3H), 7.18 (t, IH), 7.26 (t, 2H), 9.74 (br, 2H). MS (M+H) : 283.0
Example 47: (trans)-N-(2,6-difluoro-4-methoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000125_0002
1H-NMR (CDC13) 5 (ppm): 0.96 (q, IH), 1.10 (quin, IH), 1.94 (m, IH), 2.03 (br, IH), 2.32 (m, IH), 3.76 (s, 3H), 3.90 (s, 2H), 6.42 (d, 2H), 7.00 (d, 2H), 7.13 (t, IH), 7.23 (t, 2H). MS (M+H) : 290.1
Example 48: (trans)-2-phenyl-N-(4-(trifluoromethoxy)benzyl)cyclopropanamine
Figure imgf000126_0001
1H-NMR (CDC13) δ (ppm): 0.98 (q, IH), 1.11 (quin, IH), 1.91 (m, IH), 1.96 (br, IH), 2.36 (m, IH), 3.89 (d, 2H), 6.97 (d, 2H), 7.16 (d, 3H), 7.24 (t, 2H), 7.32 (d, 2H). MS (M+H) : 308.1
Example 49: (trans)-N-(5-fluoro-2-methoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000126_0002
1H-NMR (CDC13) δ (ppm): 0.96 (q, IH), 1.12 (quin, IH), 1.94 (m, IH), 2.12 (br, IH), 2.34 (m, IH), 3.78 (s, 3H), 3.86 (q, 2H), 6.76 (m, IH), 6.96 (m, 4H), 7.14 (t, IH), 7.24 (d, 2H). MS (M+H) : 272.1
Example 50: (trans)-N-(2-fluoro-4-methoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000126_0003
1H-NMR (CDC13) δ (ppm): 0.96 (q, IH), 1.12 (quin, IH), 1.88 (br, IH), 1.94 (m, IH), 2.36 (m, IH), 3.78 (s, 3H), 3.88 (s, 2H), 6.76 (m, 2H), 6.99 (d, 2H), 7.14 (m, 2H), 7.24 (m, 2H). MS (M+H) : 272.1
Example 51 : (trans)-N-((4-methoxynaphthalen-l-yl)methyl)-2- phenylcyclopropanamine
Figure imgf000127_0001
1H-NMR (CDC13) δ (ppm): 1.00 (q, IH), 1.16 (quin, IH), 1.86 (br, IH), 1.98 (m, IH), 2.46 (m, IH), 3.98(s, 3H), 4.27 (s, 2H), 6.70 (d, IH), 6.99 (d, 2H), 7.14 (t, IH), 7.24 (d, 2H), 7.32 (d, IH), 7.50 (m, 2H), 8.06 (d, IH), 8.30 (d, IH). MS (M+H) : 304.1
Example 52: (trans)-N-(2-fluoro-6-methoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000127_0002
1H-NMR (CDC13) 5 (ppm): 0.96 (q, IH), 1.12 (quin, IH), 1.92 (m, IH), 2.10 (br, IH), 2.30 (m, IH), 3.72 (s, 3H), 3.96 (s, 2H), 6.66 (m, 2H), 6.99 (d, 2H), 7.14 (m, 2H), 7.22 (m, 2H). MS (M+H) : 272.1
Example 53: (trans)-N-((2-methoxynaphthalen-l-yl)methyl)-2- phenylcyclopropanamine
Figure imgf000127_0003
1H-NMR (CDC13) δ (ppm): 0.96 (q, IH), 1.20 (quin, IH), 1.92 (br, IH), 1 .96 (m, IH), 2.40 (m, IH), 3.84 (s, 3H), 4.38 (s, 2H), 6.98 (d, 2H), 7.12 (t, IH), 7.24 (m, 3H), 7.32 (t, IH), 7.40 (t, IH), 7.80 (d, 2H), 8.02 (d, IH). MS (M+H) : 304.1 Example 54: (trans)-N-((4,7-dimethoxynaphthalen-l-yl)methyl)-2- phenylcyclopropanamine
Figure imgf000128_0001
1H-NMR (CDC13) δ (ppm): 1.01 (q, IH), 1.20 (quin, IH), 1.80 (br, IH), 2.00 (m, IH), 2.48 (m, IH), 3.92 (s, 3H), 3.96 (s, 3H), 4.24 (s, 2H), 6.60 (d, IH), 7.01 (d, 2H), 7.16 (t, 2H), 7.24 (m, 2H), 7.30 (m, IH), 7.36 (s, IH), 8.20 (d, IH). MS (M+H) : 334.0
Example 55: (trans)-N-(4-methoxy-3-methylbenzyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000128_0002
1H-NMR (DMSO-d6) 5 (ppm): 1.26 (q, IH), 1.50 (quin, IH), 2.06 (s, 3H), 2.80 (br, IH), 3.76 (s, 3H), 4.16 (s, 2H), 6.94 (d, IH), 7.10 (d, 2H), 7.28 (m, 5H), 9.50 (br, 2H). MS (M+H) : 268.0
Example 56: (trans)-N-(3-chloro-4-methoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000128_0003
1H-NMR (CDC13) δ (ppm): 0.98 (q, IH), 1.10 (quin, IH), 1.90 (m, 2H), 2.36 (m, IH), 3.80 (s, 2H), 3.90 (s, 3H), 6.86 (d, IH), 7.00 (d, 2H), 7.16 (t, 2H), 7.24 (d, 2H), 7.32 (s, IH). MS (M+H) : 287.9
Example 57: (trans)-N-(3-fluoro-4-methoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000129_0001
1H-NMR (CDC13) δ (ppm): 0.96 (q, IH), 1.10 (quin, IH), 1.92 (m, 2H), 2.36 (m, IH), 3.80 (s, 2H), 3.88 (s, 3H), 6.90 (t, IH), 6.99 (d, 3H), 7.06 (d, IH), 7.16 (d, IH), 7.24 (d, 2H). MS (M+H) : 271.96
Example 58: (trans)-N-(4-methoxy-2-methy]benzyl)-2-phenylcyclopropanamine
Figure imgf000129_0002
1H-NMR (CDC13) δ (ppm): 0.98 (q, IH), 1.12 (quin, IH), 1.82 (br, IH), 1.94 (m, IH), 2.34 (s, 3H), 2.40 (m, IH), 3.78 (s, 3H), 3.82 (s, 2H), 6.68 (d, IH), 6.72 (s, IH), 7.01 (d, 2H), 7.14 (t, 2H), 7.24 (d, 2H). MS (M+H) : 268.0
Example 59: (trans)-N-((3,4-dihydro-2H-benzo[b][l,4]dioxepin-6-yl)methyl)-2- phenylcyclopropanamine
Figure imgf000129_0003
1H-NMR (CDC13) δ (ppm): 0.98 (q, IH), 1.14 (quin, IH), 1.86 (br, IH), 1.90 (m, IH), 2.18 (m, 2H), 2.36 (m, IH), 3.88 (s, 2H), 4.20 (s, 4H), 6.88 (m, 3H), 7.00 (d, 2H), 7.14 (t, IH), 7.24 (d, 2H). MS (M+H) : 295.9
Example 60: (trans)-N-((3,4-dihydro-2H-benzo[b][l ,4]dioxepin-7-yl)methyl)-2- phenylcyclopropanamine hydrochloride
Figure imgf000130_0001
1H-NMR (DMSO-d6) δ (ppm): 1.25 (q, IH), 1.46 (m, IH), 2.08 (m, 2H), 2.38 (m, IH), 2.88 (m, IH), 4.10 (m, 4H), 4.17 (s, 2H), 6.96 (d, IH), 7.10 (m, 3H), 7.13 (s, IH), 7.19 (t, IH), 7.26 (t, 2H), 9.38 (br, 2H). MS (M+H) : 295.9
Example 61 : (trans)-N-((2,2-dimethylchroman-6-yl)rnethyl)-2- phenylcyclopropanamine
Figure imgf000130_0002
1H-NMR (CDC13) δ (ppm): 0.96 (q, IH), 1.14 (quin, IH), 1.32 (s, 6H), 1.78 (t, 2H), 1.94 (m, 2H), 2.38 (m, IH), 2.70 (t, 3H), 3.78 (s, 2H), 6.72 (d, IH), 7.00 (m, 4H), 7.14 (t, IH), 7.24 (d, 2H). MS (M+H) : 308.1
Example 62: (trans)-N-(4-methoxy-2,3-dimethylbenzyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000131_0001
1H-NMR (DMSO-d6) δ (ppm): 1.28 (q, IH), 1.52 (quin, IH), 2.06 (s, 3H), 2.20 (s, 3H), 2.42 (m, IH), 2.94 (br, IH), 3.76 (s, 3H), 4.26 (s, 2H), 6.84 (d, IH), 7.09 (d, 2H), 7.20 (t, IH), 7.28 (d, 3H), 9.32 (br, 2H). MS (M+H) : 282.1
Example 63: (trans)-N-(4-methoxy-2,5-dimethylbenzyl)-2-phenylcyclopropan amine
Figure imgf000131_0002
1H-NMR (CDC13) δ (ppm): 0.98 (q, IH), 1.14 (quin, IH), 1.82 (br, IH), 1.94 (m, IH), 2.12 (s, 3H), 2.32 (s, 3H), 2.38 (m, IH), 3.80 (s, 5H), 6.62 (s, IH), 6.97 (s, IH), 7.01 (d, 2H), 7.14 (t, IH), 7.24 (d, 2H). MS (M+H) : 282.1
Example 64: (trans)-N-(2-fluoro-4,5-dimethoxybenzy])-2-phenylcyclopropanamine
Figure imgf000131_0003
1H-NMR (CDC13) δ (ppm): 0.96 (q, IH), 1.14 (quin, IH), 1.92 (br, IH), 2.00 (br, IH), 2.34 (br, IH), 3.68 (s, 2H), 3.84 (s, 6H), 6.60 (d, IH), 6.70 (d, IH), 6.99 (d, 2H), 7.14 (q, IH), 7.24 (m, 2H). MS (M+H) : 301.99 Example 65: (trans)-N-(3-chloro-4,5-dimethoxybenzyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000132_0001
1H-NMR (DMSO-d6) δ (ppm): 1.28 (q, IH), 1.48 (m, IH), 2.36 (m, IH), 2.88 (m, IH), 3.72 (s, 6H), 4.24 (s, 2H), 7.08 (d, 2H), 7.20 (s, 2H), 7.28 (d, 3H). MS (M+H) : 318.0
Example 66: (trans)-N-(2-chloro-3,4-dimethoxybenzyl)-2-phenylcyclopropanamine
Figure imgf000132_0002
1H-NMR (CDC13) 5 (ppm): 0.98 (q, IH), 1.16 (quin, IH), 2.00 (m, IH), 2.36 (m, IH), 3.88 (s, 6H), 3.96 (s, 2H), 6.76 (d, IH), 7.02 (m, 3H), 7.16 (t, IH), 7.24 (d, 2H). MS (M+H) : 318.0
Example 67: (trans)-N-(2,4-dimethoxy-6-methylbenzyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000132_0003
1H-NMR (DMSO-d6) δ (ppm): 1.28 (q, IH), 1.48 (quin, IH), 2.32 (s, 3H), 2.38 (m, IH), 2.90 (m, IH), 3.72 (s, 3H), 3.76 (s, 3H), 4.14 (s, 2H), 6.42 (s, 2H), 7.11 (d, 2H), 7.22 (t, IH), 7.28 (t, 2H), 8.96 (br, 2H). MS (M+H) : 298.0
Example (trans)-N-(2,5-dimethoxybenzyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000133_0001
1H-NMR (DMSO-d6) 5 (ppm): 1.22 (q, IH), 1.48 (quin, IH), 2.36 (m, IH), 2.80 (m, IH), 3.68 (s, 6H), 4.16 (s, 2H), 6.92 (s, 2H), 7.08 (m, 3H), 7.18 (t, IH), 7.28 (t, 2H), 9.30 (br, 2H). MS (M+H) : 284.0
Example 69: (trans)-N-(2,3-dimethoxybenzyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000133_0002
1H-NMR (DMSO-d6) δ (ppm): 1.26 (q, IH), 1.46 (quin, IH), 2.38 (m, IH), 2.88 (m, IH), 3.76 (s, 3H), 3.80 (s, 3H), 4.21 (s, 2H), 7.09 (t, 5H), 7.20 (t, IH), 7.26 (t, 2H). MS (M+H) : 284.0
Example 70: (trans)-N-(2-chloro-3-methoxybenzyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000134_0001
1H-NMR (DMSO-d6) δ (ppm): 1.28 (q, IH), 1.56 (quin, IH), 2.48 (m, IH), 2.92 (m, IH), 3.86 (s, 3H), 4.37 (s, 2H), 7.09 (d, 2H), 7.21 (t, 2H), 7.28 (t, 3H), 7.37 (t, IH). MS (M+H) : 287.9
Example 71 : (trans)-N-((lH-indol-5-yl)methyl)-2-phenylcyclopropanamine hydrochloride
Figure imgf000134_0002
1H-NMR (DMSO-d6) δ (ppm): 1.26 (q, IH), 1.50 (quin, IH), 2.48 (m, IH), 2.86 (m, IH), 3.86 (s, 3H), 4.32 (s, 2H), 6.40 (s, IH), 7.09 (d, 2H), 7.24 (m, 4H), 7.38 (t, 2H), 7.67 (s, IH), 9.46 (br, IH). MS (M+H) : 287.9
Following examples have been synthesized using the procedure described for Example 28 and the corresponding starting materials.
Example 72: (trans)-2-(4-(benzyloxy)phenyl)-N-(pyridin~2- ylmethyl)cyclopropanamine
Figure imgf000134_0003
1H-NMR (CD3OD) δ (ppm): 0.91 (q, IH), 1.02 (quin, IH), 1.84 (m, IH), 2.22 (m, IH), 3.96 (s, 2H), 5.02 (s, 2H), 6.87 (q, 4H), 7.38 (m, 8H), 7.78 (t, IH), 8.49 (d, IH). MS (M+H) : 331.1
Example 73: (trans)-2~(4-(benzyloxy)phenyl)-N-(2- methoxybenzyl)cyclopropanamine
Figure imgf000135_0001
1H-NMR (CD3OD) 6 (ppm): 1.29 (q, IH), 1.38 (quin, IH), 2.27 (m, IH), 2.84 (m, IH), 3.78 (s, 3H), 4.33 (s, 2H), 5.04 (s, 2H), 6.96 (m, 6H), 7.36 (m, 7H). MS (M+H) : 360.3
Example 74: (trans)-N-(l-(4-methoxyphenyl)ethyl)-2-phenylcyclopropanamine
Figure imgf000135_0002
l-(4-methoxyphenyl)ethanone ( 138 mg, 0.75 mmol) and molecular sieve (3 A - previously activated by microwaves) was added to a solution of (trans)-2- phenylcyclopropanamine hydrochloride in 2 rnL of MeOH anh,. HCl 2N in dioxane (2 drops) was added and the reaction was stirred at room temperature for 3 hours. The reaction was cooled to 00C and NaBH3CN ( 99 mg, 1.5 mmol) was added. It was stirred overnight at room temperature.
A solution of NH4Cl sat. (2 mL) was added. After removal of the solvent the crude was dissolved in CH2C12 and washed with a aqueous saturated solution of NH4Cl. It was finally washed with brine and the extracted organic layer was dried over MgSO4 anh. The crude was purified by chromatography using Hexane-AcOEt (80:20 to 70:30 in 10 min) to get colourless oil, 102 mg (yield = 48.1%)
1H-NMR (CDC13) δ (ppm): 0.90 (q, IH), 0.96 (quin, IH), 1.42 (s, 3H), 2.00 (br, IH), 2.20 (m, IH), 3.78 (d, 3H), 3.92 (m, IH), 6.84 (q, 3H), 7.00 (d, IH), 7.16 (m, 2H), 7.24 (d, 3H). MS (M+H) : 268.0
Following examples have been synthesized using the procedure described for Example 74 and the corresponding starting materials.
Example 75: (trans)-N-(l-(3,4-dimethoxyphenyl)ethyl)-2-phenylcyclopropanamine
Figure imgf000136_0001
1H-NMR (CDC13) δ (ppm): 0.94 (m, IH), 1.08 (br, IH), 1.40 (s, 3H), 1.76 (br, 2H), 2.20 (m, IH), 3.76 (s, IH), 3.85 (t, 6H), 6.80 (m, 4H), 7.00 (d, IH), 7.18 (m, 3H). MS (M+H) : 298.0
Example 76: (trans)-N-(l-(2,3-dihydrobenzo[b] [l ,4]dioxin-6-yl)ethyl)-2- phenylcyclopropanamine
Figure imgf000136_0002
1H-NMR (CDC13) δ (ppm): 0.90-0.96 (m, IH), 1.08 (br, IH), 1.38 (d, 3H), 1.84-1.96 (br, IH), 2.19-2.23 (m, IH), 3.84 (m, IH), 4.24 (s, 4H), 6.82 (m, 4H), 7.00 (d, IH), 7.20 (m, 3H). MS (M+H) : 296.0
Example 77: (trans)-N-(l-(5-fluoro-2-methoxyphenyl)ethyl)-2- phenylcyclopropanamine
Figure imgf000137_0001
1H-NMR (CDC13) δ (ppm): 0.92 (q, IH), 1.06 (m, IH), 1.36 (d, 3H), 1.80-1.92 (m, IH), 2.18 (m, IH), 3.70 (s, 3H), 4.22-4.28 (m, IH), 6.74 (m, IH), 6.86 (m, 2H), 6.99 (m, 2H), 7.12 (m, IH), 7.20 (m, 2H). MS (M+H) : 286.0
Example 78: (trans)-N-(l-(3,4-dimethoxyphenyl)propan-2-yl)~2- phenylcyclopropanamine
Figure imgf000137_0002
1H-NMR (CDC13) δ (ppm): 0.96 (q, IH), 1.06 (m, IH), 1.14 (d, 3H), 1.79- 1.86 (m, IH), 2.18-2.32 (m, IH), 2.63-2.71 (m, 2H), 3.04 (m, IH), 3.85 (d, 6H), 6.70 (m, 2H), 6.79 (d, IH), 7.02 (m, 2H), 7.13 (m, IH), 7.23 (m, 2H). MS (M+H) : 312.1
Example 79: (trans)-N-((3-methyl-l ,2,4-oxadiazol-5-yl)methyl)-2- phenylcyclopropanamine hydrochloride
Figure imgf000137_0003
Step 1:
N,N-Diisopropylethylamine (3 rnL, 17.16 mmol) was added to a solution of (trans)- 2-phenylcyclopropanamine hydrochloride (1 ,5 g , 8.58 mmol) in 70 niL of CH3CN. After complete dissolution of the amine the methyl 2-bromoacetate (930 μL, 9.44 mmol) was added and then stirred overnight at room temperature. After solvent removal the crude was purified by flash chromatography eluting with CH2CbZMeOH (99: 1 to 90: 10) to get methyl 2-((trans)-2-phenylcyclopropylamino)acetate as colourless oil (1.76 g, Yield = 100%).
Step 2:
A solution of LiOH (432 mg, 10.30 mmol) in 17 mL of H2O was added to the solution of methyl 2-((trans)-2-phenylcyclopropylamino)acetate (1.76 g, 8.58 mmol) in 70 mL de THF and it was stirred vigorously for 2 hours at room temperature. Di- tert-butyl dicarbonate (2.7 g, 12 mmol) was added and stirred for 3 hours. After solvent removal the crude was solved in 60 mL of AcOEt and washed with 40 mL of brine. The organic layer was dried over MgSO4 anh., filtered and the solvent was removed. The crude was purified by flash chromatography eluting with CH2C12/MeOH (98:2 to 90: 10) to get 2-(tert-butoxycarbonyl((trans)-2- phenylcyclopropyl)amino)acetic acid as colourless oil (2.08 g. Yield = 83.3%).
Step 3:
EDC (107 μL, 0.585 mmol) and acetoamidoxime (41 mg, 0.532 mmol) was added to a stirred solution of 2-(tert-butoxycarbonyl((trans)-2- phenylcyclopropyl)amino)acetic acid (163 mg, 0.532 mmol) in diglyme (2 mL) under argon atmosphere. The mixture was stirred at 50 0C overnight and then at 110 0C for 14 hours. After removal of solvent under vacuum, the reaction mixture was purified by flash cromatography eluting with hexane/MTBE (80:20 to 0: 100) to afford tert-butyl (3-methyl- l,2,4-oxadiazol-5-yl)methyl((l S,2S)-2- phenylcyclopropyl)carbamate as a colourless oil (35 mg. Yield = 20%).
Step 4:
HCl 2N in Et2O was added to tert-butyl (3-methyl-l ,2,4-oxadiazol-5- yl)methyl((l S,2S)-2-phenylcyclopropyl)carbamate (35 mg, 0.106 mmol). The Et2O was decanted and the solid was washed twice with 5 mL of Et2O. The solid was dried by vacuum to get (trans)-N-((3-methyl-l ,2,4-oxadiazol-5-yl)methyl)-2- phenylcyclopropanamine hydrochloride (14 mg. Yield 12.8%) 1H-NMR (DMSO-d6) δ (ppm): 1.28 (q, IH), 1.52 (quin, IH), 2.35 (s, 3H), 3.00 (m, IH), 4.71 (s, 2H), 7.12 (d, 2H), 7.20 (t, IH), 7.28 (t, 2H). MS (M+H) : 229.9
Example 80: Biological Assays - LSDl
The compounds of the invention can be tested for their ability to inhibit LSD l . The ability of the compounds of the invention to inhibit LSDl can be tested as follows. Human recombinant LSDl protein was purchased from BPS Bioscience Inc. In order to monitor LSDl enzymatic activity and/or its inhibition rate by our inhibitor(s) of interest, di-methylated H3-K4 peptide (Millipore) was chosen as a substrate. The demethylase activity was estimated, under aerobic conditions, by measuring the release of H2O2 produced during the catalytic process, using the Amplex® Red pεroxide/peroxidase-coupled assay kit (Invitrogen).
Briefly, a fixed amount of LSDl was incubated on ice for 15 minutes, in the absence and/or in the presence of various concentrations of inhibitor (from 0 to 75 μM, depending on the inhibitor strength). Tranylcypromine (Biomol International) was used as a control for inhibition. Within the experiment, each concentration of inhibitor was tested in triplicate. After leaving the enzyme interacting with the inhibitor, 12.5 μM of di-methylated H3-K4 peptide was added to each reaction and the experiment was left for 1 hour at 370C in the dark. The enzymatic reactions were set up in a 50 mM sodium phosphate, pH 7.4 buffer. At the end of the incubation, Amplex® Red reagent and horseradish peroxidase (HPR) solution were added to the reaction according to the recommendations provided by the supplier (Invitrogen), and left to incubate for 30 extra minutes at room temperature in the dark. A 1 μM H2O2 solution was used as a control of the kit efficiency. The conversion of the Amplex® Red reagent to resorufin due to the presence of H2O2 in the assay, was monitored by fluorescence (excitation at 540 nm, emission at 590 nm) using a microplate reader (Infinite 200, Tecan). Arbitrary units were used to measure level Of H2O2 produced in the absence and/or in the presence of inhibitor.
The maximum demethylase activity of LSDl was obtained in the absence of inhibitor and corrected for background fluorescence in the absence of LSDl . The Ki of each inhibitor was estimated at half of the maximum activity. A number of the compounds of the invention were tested for their ability to inhibit LSDl and were found to have Ki values lower than 100 μM, including many of the compounds in examples that were tested. Compound of examples 3 and 17, were found to have Ki values for LSDl of less than 10 μM. Compounds of examples 1 , 8, 9, 11 were found to have Ki values for LSDl of less than 1 micromolar. Parnate (2- trans phenylcyclopropylamine) was found to have a Ki of from about 15 to 35 micromolar depending on the enzyme preparation.
Previous studies reported in the literature indicated that substitutions on the amine group of phenylcyclopropylamines reduced the ability of the compound to inhibit monoamine oxidases, which have significant structural homology to LSDl . For example Zirkle et al. ((1962) J. Med. Chem. 1265-1284) found that a methyl substituent on the amine group decreased activity slightly whereas substitution with larger alkyl groups and groups bearing ring ring system like aralkyls reduced MAO activity substantially. The inventors of the instant invention have surprisingly found that ring bearing substitutions on the amine group of phenylcyclopropyl amine produce potent LSDl inhibitors.
Previous reports of LSDl have found that it is involved in cell proliferation and growth. Some studies have implicated LSDl as a therapeutic target for cancer. Huang et al. (2007) PNAS 104:8023-8028 found that polyamines inhibitors of LSDl modestly cause the reexpression of genes aberrantly silenced in cancer cells and particularly colorectal cancer (Huang et al. Clin Cancer Res. (2009) Dec l ; 15(23):7217-28. Epub 2009 Nov 24. PMID: 19934284). Scoumanne et al. ((2007) J. Biol. Chem. May 25;282(21): 15471 -5) found that deficiency in LSD l leads to a partial cell cycle arrest in G2/M and sensitizes cells to growth suppression induced by DNA damage. Kahl et al. ((2006) Cancer Res. 66(23): 11341 -7.) found that LSDl expression is correlated with prostate cancer aggressiveness. Metzger et al. reported that LSDl modulation by siRNA and pargyline regulates androgen receptor (AR) and may have therapeutic potential in cancers where AR plays a role, like prostate, testis, and brain cancers. Lee et al ((2006) Chem. Biol. 13 :563-567) reported that tranylcypromine derepresses Egr-1 gene expression in some cancer lines. A body of evidence is accumlating that Egr-1 is a tumor suppressor gene in many contexts (see e.g., Calogero et al. (2004) Cancer Cell International 4: 1 exogenous expression of EGR-I resulted in growth arrest and eventual cell death in primary cancer cell lines; Lucerna et al. (2006) Cancer Research 66, 6708-6713 show that sustained expression of Egr-1 causes antiangiogeneic effects and inhibits tumor growth in some models; Ferraro et al. ((2005) J. Clin. Oncol. Mar 20;23(9): 1921-6) reported that Egr-1 is downregulated in lung cancer patients with a higher risk of recurrence and may be more resistant to to therapy. Thus, increasing Egr-1 expression via inhibition of LSDl is a therapeutic approach for some cancers. Recent studies have also implicated LSDl in brain cancer (Schulte et al. (2009) Cancer Res. Mar l ;69(5):2065-71 ). Other studies have implicated LSD l in breast cancer (Lims et al. Carcinogenesis. 2009 Dec 30. [Epub ahead of print] PMID: 20042638).
Thus, a body of evidence has implicated LSD l in a number of cancers, which suggests that LSDl is a therapeutic target for cancer. The instant inventors have discovered a class of LSD l inhibitors that can be used to treat diseases where LSDl is implicated as a therpautic target like cancer. According, the phenylcyclopropylamine compounds of the invention can be used to treat and/or prevent such diseases.
Example 81 : Biological Assays - Monoamine Oxidase Assays
Human recombinant monoamine oxidase proteins MAO-A and MAO-B were purchased from Sigma Aldrich. MAOs catalyze the oxidative deamination of 1°, 2° and 3° amines. In order to monitor MAO enzymatic activities and/or their inhibition rate by inhibitor(s) of interest, a fluorescent-based (inhibitor)-screening assay was set up. 3-(2-Aminophenyl)-3- oxopropamamine (kynuramine dihydrobromide, Sigma Aldrich), a non fluorescent compound was chosen as a substrate. Kynuramine is a non-specific substrate for both MAOs activities. While undergoing oxidative deamination by MAO activities, kynuramine is converted into 4-hydroxyquinoline (4-HQ), a resulting fluorescent product.
The monoamine oxidase activity was estimated by measuring the conversion of kynuramine into 4-hydroxyquinoline. Assays were conducted in 96-well black plates with clear bottom (Corning) in a final volume of 100 μL. The assay buffer was 100 mM HEPES, pH 7.5. Each experiment was performed in triplicate within the same experiment.
Briefly, a fixed amount of MAO (0.25 μg for MAO-A and 0.5 μg for MAO-B) was incubated on ice for 15 minutes in the reaction buffer, in the absence and/or in the presence of various concentrations of inhibitor (from 0 to 50 μM, depending on the inhibitor strength). Tranylcypromine (Biomol International) was used as a control for inhibition.
After leaving the enzyme(s) interacting with the inhibitor, 60 to 90 μM of kynuramine was added to each reaction for MAO-B and MAO-A assay respectively, and the reaction was left for 1 hour at 37°C in the dark. The oxidative deamination of the substrate was stopped by adding 50 μL (v/v) of NaOH 2N. The conversion of kynuramine to 4-hydroxyquinoline, was monitored by fluorescence (excitation at 320 nm, emission at 360 nm) using a microplate reader (Infinite 200, Tecan). Arbitrary units were used to measure levels of fluorescence produced in the absence and/or in the presence of inhibitor.
The maximum of oxidative deamination activity was obtained by measuring the amount of 4- hydroxyquinoline formed from kynuramine deamination in the absence of inhibitor and corrected for background fluorescence in the absence of MAO enzymes. The Ki of each inhibitor was measure at Vmax/2.
Using the above described assay a number of the compounds of the invention were tested for their ability to inhibit MAO-B and were found to have Ki values lower than 100 μM, including many of the compounds in examples tested. Compound of examples 1 , 4, 16, 1 1 , and 17 were found to have Ki (IC50) values for MAO-B of less than 100 μM. Trans-2- phenylcyclopropylamine (tranylcypromine) was found to have a Ki for MAO-A of about 2 micromolar and a Ki of about 0.6 micromolar for MAO-B.
Previous reports in the literature (Zirkle et al. (1962) J. Med. Chem. 1265-1284) indicated that substitutions on the amine group of phenylcyclopropylamine with small alkyl groups like methyl reduce MAO inhibitory activity slightly whereas disubstitution of the amine with methyl or mono substitution with larger ring bearing substituents like benzyl reduce MAO inhibitory activity substantially. The inventors of the instant invention found that larger ring bearing substituents on the amine group of phenylcyclopropylamine could produce potent inhibitors of monoamine oxidases and particularly MAO-B.
A number of the compounds of the invention were tested for their ability to inhibit MAO-A using the above described assay and were found to have Ki values for MAO-A higher than that for MAO-B (i.e., inhibit MAO-B better than MAO-A) e.g., like the compounds of Examples 11 and 13 which both a better MAO-B inhbititors than MAO-A inhbitors which MAO-B IC50 values below 50 micromolar.
Thus, unexpectedly compounds having large ring bearing substituents on the amine group of phenylcyclopropylamine were found to be potent LSDl inhibitors as well as potent MAO-B inhibitors.
The invention therefore provides inhibitors selective for LSDl . LSDl selective inhibitors have IC50 values for LSDl which are at least 2-fold lower than the IC50 value for MAO-A and/or MAO-B. One example of an LSDl selective inhibitor is given in Example 2 which has an IC50 for LSD l which is about 10-fold lower than for MAO-A and MAO-B. Another example of an LSDl selective inhibitor is in Example 16 which has an IC50 for LSDl which is more than 5-fold lower than the IC50 for MAO-A and MAO-B. Yet another example of a selective LSDl inhibitor is given in Example 17 which has an IC50 which is more than 3-fold lower for LSDl than MAO-A and MAO-B.
The invention also provides dual inhibitors selective for LSDl and MAO-B. Dual LSD1/MAO-B selective inhibitors have IC50 values for LSDl and MAO-B which are at least 2-fold lower than the IC50 value for MAO-A. One example of a dual LSD1/MA0-B selective inhibitor is given in Example 11 which has an IC50 for LSD l and MAO-B which is about 2-fold lower than for MAO-A. Another example of a dual LSDl /MAO-B inhibitor is iven in Example 7 where the MAO-B IC50 is less than half the value it is for MAO-A and the LSDl IC50 is about 1 micromolar. Table 1
IC50 Values (micromolar) for Selected Examples
Figure imgf000144_0001
Figure imgf000145_0001
Figure imgf000146_0001
All results reported in Table 1 are the average of at-least two determinations of the IC50 value and usually more, unless otherwise noted, and nd signifies not determined. The IC50 LSDl values for the inhibitors of examples 20, 21 , 23, and 25 represent only one measurement.
As can be seen from Table 1 , the compounds of Formula I can have IC50 values for LSDl of less than that for parnate, less than 10 micromolar, less than 1 micromolar, and less than 500 nanomolar. Typically the IC50 values of the compounds of Formula I for LSDl are less than 1 micromolar.
As can be seen from Table 1 , the compounds of Formula I can have IC50 values for MAO-A of less than 20 micromolar, less than 10 micromolar, and less than 5 micromolar. Typically the IC50 values of the compounds of Formula I for MAO-A are greater than 1 micromolar.
As can be seen from Table 1 , the compounds of Formula I can have IC50 values for MAO-B of less than 20 micromolar, less than 10 micromolar, and less than 5 micromolar. Typically the IC50 values of the compounds of Formula I for MAO-B are greater than 1 micromolar. Most of the compounds of the Examples are selective LSDl inhibitors in that they inhibit LSD l to a greater extent than MAO-A and MAO-B. Some of the compounds of the Examples inhibit both MAO-B and LSDl to a greater extent than MAO-A.
As the skilled artisan readily recognizes the compounds disclosed herein are surprisingly and significantly more potent than tranylcypromine for LSD l inhibition. Han et al. (Euro. J. Pharma. (2008) doi: 10.1016/j.ejphar.2008.12.025) reported that phenylcyclopropylamine displays neuroprotective activity in PC 12 cells thus the compounds of Formula I may be used as a neuroprotectant (e.g., used to treat and/or prevent conditions characterized by neurodegeneration). Furthermore, since the compounds of Formula I are potent LSDl inhibitor they can be used to treat and/or prevent diseases where LSDl inhibition is desirable, e.g., cancer.
All publications and patent applications mentioned in the specification are indicative of the level of those skilled in the art to which this invention pertains. All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. The mere mentioning of the publications and patent applications does not necessarily constitute an admission that they are prior art to the instant application.
Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims.

Claims

New PCT Patent Application 147 Oryzon Genomics S. A. Our Ref.: P3583 PCT S3 CLAIMS
1. A compound of Formula I for use in treating or preventing cancer:
Figure imgf000148_0001
FORMULA 1 wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, suifinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is a -L-heterocyclyl wherein the ring or ring system of said -L- heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, suifinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; or R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CHi)n-(CHi)n-, -(CHi)nNH(CHi)n-, -(CHi)nO(CHi)n-, and -(CHi)nS(CHi)n-, and where each n is independently chosen from 0, 1 , 2, and 3; or a pharmaceutically acceptable salt thereof.
2. The compound of claim 1 wherein R8 is a -L-heterocyclyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L- heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O-thiocarbamyl, N- thiocarbamyl, and C-amido.
3. The compound of claim 1 wherein R8 is -L-heterocyclyl wherein said -L-heterocyclyl is -L-heteroaryl wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido.
4. The compound of claim 1 wherein R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1 -3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L- carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido.
5. The compound of any of claims 1 -4 wherein L is independently chosen from -(CH2)n-(CH2)n- and -(CHi)nO(CH2)H-, wherein each n is independently chosen from 0, 1 , 2, and 3.
6. The compound of any of claims 1 -5 wherein L is -CH2- or a covalent bond.
7. The compound of any of claims 1 -6 wherein L is a covalent bond.
8. The compound of any of claims 1 -7 wherein R6 and R7 are hydro.
9. The compound of any of claims 1 -8 wherein at least one of Rl to R5 is not hydro.
10. The compound of any of claims 1 -8 wherein one of R1 -R5 is chosen from -L-aryl, -L-heterocyclyl, and -L-carbocyclyl.
11. The compound of any of claims 1-10 wherein the substituent or the substituents on the R8 ring or ring system are chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Ci-3 alkyl)2, -NH(Ci-3 alkyl), -C(=O)NH2, -C(=O)NH(Ci-3 alkyl), - C(=O)N(Ci-3 alkyl)2, -SC=O)2(C1. 3alkyl), -S(=O)2NH2, -S(O)2N(C1-3 alkyl)2, -S(=O)2NH(Ci-3 alkyl), -CN, -NH2, and -NO2.
12. The compound of any of claims 1 -11 wherein the R8 ring or ring system has 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
13. The compound of any of claims 1 -12 wherein said cancer is chosen from breast cancer, lung cancer, prostate cancer, testicular cancer, brain cancer, colorectal cancer, blood cancer, and skin cancer.
14. A compound of Formula I:
Figure imgf000151_0001
FORMULA 1 wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is a -L-heterocyclyl wherein the ring or ring system of said -L- heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; or R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CH2)O-(CH2)I1-, -(CH2)nNH(CH2)n-, -(CH2)nO(CH2)n-, and -(CH2)nS(CH2)n-, and each n is independently chosen from 0, 1 , 2, and 3; or a pharmaceutically acceptable salt thereof; with the provision that when L is a bond and Rl , R2, R3, R4, R5, R6, and R7 are all hydro, then R8 is not 2,4-dimethoxyphenyl, 4-nitrophenyl, phenyl, 4-bromophenyl, 4-methoxyphenyl, 4-chlorophenyl, or furan-2-yl, and also when Rl , R2, R3, R4, R5, and R7 are all hydro, L is a bond and R8 is phenyl, then R6 is not methyl, ethyl, or isopentyl.
15. The compound of claim 14 wherein R6 and R7 are each hydro.
16. The compound of claim 14 or 15 wherein L is independently chosen from -(CH2)n-(CH2)n- and -(CH2)nO(CH2)n-, wherein each n is independently chosen from 0, 1 , 2, and 3.
17. The compound of any of claims 14-16 wherein L is -CH2- or a covalent bond.
18. The compound of any of claims 14-17 wherein L is a covalent bond.
19. The compound of any of claims 16-18 wherein R8 is an -L-heterocycyl wherein the ring or ring system of said -L-heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido.
20. The compound of any of claims 14-18 wherein R8 is an -L-heterocyclyl wherein said -L-heterocycyl is a -L-heteroaryl and wherein the ring or ring system of said -L-heteroaryl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, - L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido.
21. The compound of any of claims 14-18 wherein R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, - L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido.
22. The compound of claim 21 wherein R8 is -L-aryl wherein the ring or ring system of said -L-aryl has 3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido.
23. The compound of any of claims 14-22 wherein one of Rl , R2, R3, R4, and R5 is not a hydro.
24. The compound of any of claims 14-22 wherein one of Rl , R2, R3, R4, and R5 is chosen from -L-heterocyclyl, -L-aryl, and -L-carbocyclyl.
25. The compound of any of claims 14-24 wherein the substituent or the substituents on the R8 ring or ring system are chosen from hydroxyl, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -N(Cj-3 alkyl)2, -NH(Ci-3 alkyl), -C(=O)NH2, -C(O)NH(CL3 alkyl), -C(O)N(CL3 alkyl)2, -S(O)2(Ci.3alkyl), -S(=O)2NH2, -S(O)2N(C1 -3 alkyl)2, -S(O)2NH(CL3 alkyl), -CN, -NH2, and -NO2.
26. The compound of any of claims 14-21, 23 or 24 wherein the R8 ring or ring system has 1 substituent chosen from halo, alkyl, alkoxy, haloalkyl, cyano, and hydroxyl.
27. The compound of any of claims 1-26 wherein the substituents on the cyclopropyl ring have the trans configuration.
28. The compound of claim 1 or 14 wherein said compound is chosen from: (trans)-N-(4-fluorobenzyl)-2-phenylcyclopropanamine; (trans)-N-(4-fluorobenzyl)-2-phenylcyclopropanaminium; 4-(((trans)-2-phenylcyclopropylamino)methyl)benzonitrile;
(trans)-N-(4-cyanobenzyl)-2-phenylcyciopropanaminium; (trans)-2-phenyl-N-(4-(trifluoromethyl)benzyl)cyclopropanamine; (trans)-2-phenyl-N-(4-(trifluoromethyl)benzyl)cyclopropanaminium;
(trans)-2-phenyl-N-(pyridin-2-ylmethyl)cyclopropanamine;
(trans)-2-phenyl-N-(pyridin-3-ylmethyl)cyclopropanamine;
(trans)-2-phenyl-N-(pyridin-4-ylmethyl)cyclopropanamine;
(trans)-N-((6-methylpyridin-2-yl)methyl)-2-phenylcyclopropanamine;
(trans)-2-phenyl-N-(thiazol-2-ylmethyl)cyclopropanamine;
(trans)-2-phenyl-N-(thiophen-2-ylmethyl)cyclopropanamine;
(trans)-N-((3-bromothiophen-2-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((4-bromothiophen-2-yl)methyl)-2-phenylcyclopropan amine;
(trans)-N-(3,4-dichlorobenzyl)-2-phenylcyclopropanamine;
(trans)-N-(3-fluorobenzyl)-2-phenylcyclopropanaminium;
(trans)-N-(2-fluorobenzyl)-2-phenylcyclopropanamine;
(trans)-2-phenyl-N-(quinolin-4-ylmethyl)cyclopropanamine;
(trans)-N-(3-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-2-phenyl-N-((6-(trifluoromethyl)pyridin-3- yl)methyl)cyclopropanamine;
(trans)-N-((6-chloropyridin-3-yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-((4-methylpyridin-2-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((6-methoxypyridin-2-yl)methyl)-2-phenylcyclopropanamine;
2-(((trans)-2-phenylcyclopropylamino)methyl)pyridin-3-ol;
(trans)-N-((6-bromopyridin-2-yl)methyl)-2-phenylcyclopropanamine;
4-(((trans)-2-(4-(benzyloxy)phenyl)cyclopropylamino)methyl)benzonitrile;
(trans)-N-(4-(benzyloxy)benzyl)-2-phenylcyclopropanamine;
(trans)-N-benzyl-2-(4-(benzyloxy)phenyl)cyclopropanamine;
(trans)-2-(4-(benzyloxy)phenyl)-N-(4- methoxybenzyl)cyclopropanamine;
(trans)-2-(4-(benzyloxy)phenyl)-N-(4- fluorobenzyl)cyclopropanamine; and pharmaceutically acceptable salts thereof.
29. The compound of claim 1 or 14 wherein said compound is chosen from: (trans)-2-phenyl-N-(quinolin-2-ylmethyl)cyclopropanamine; (trans)-2-phenyl-N-((5-(trifluoromethyl)pyridin-2- yl)methyl)cyclopropanamine; (trans)-N-((3-fluoropyridin-2-yl)methyl)-2-phenylcyclopropanamine; (trans)-2-phenyl-N-(quinolin-3-ylmethyl)cyclopropanamine;
(trans)-N-((6-methoxypyridin-3-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((5-methoxypyridin-3-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((2-methoxypyridin-3-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((3H-indol-3-yl)methyl)-2-phenylcyclopropanamine;
3-(((trans)-2-phenylcyclopropylamino)methyl)benzonitrile;
(trans)-N-(2-methoxybenzyl)-2-phenylcyclopropanamine;
3-(((trans)-2-phenylcyclopropylamino)methyl)pyridin-2-amine;
(trans)-N-((2-chloropyridin-3-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(3,4-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-((2,3-dihydrobenzofuran-5-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(benzo[d][l,3]dioxol-5-ylmethyl)-2-phenylcyclopropanamine;
(trans)-N-((2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-(2,6-difluoro-4-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-2-phenyl-N-(4-(trifluoromethoxy)benzyl)cyclopropanamine;
(trans)-N-(5-fluoro-2-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2-fluoro-4-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-((4-methoxynaphthalen-l -yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(2-fluoro-6-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-((2-methoxynaphthalen-l -yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-((4,7-dimethoxynaphthalen- 1 -yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-(4-methoxy-3-methylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-(3-chloro-4-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(3-fluoro-4-methoxybenzyl)-2-phenylcyclopropan amine;
(trans)-N-(4-methoxy-2-methylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-((3,4-dihydro-2H-benzo[b] [l ,4]dioxepin-6-yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-((3,4-dihydro-2H-benzo[b] [l ,4]dioxepin-7-yl)methyl)-2- phenylcyclopropanamine;
(trans)-N-((2,2-dimethylchroman-6-yl)methyl)-2-phenylcyclopropanamine;
(trans)-N-(4-methoxy-2,3-dimethylbenzyl)-2-phenylcyclopropanamine; (trans)-N-(4-methoxy-2,5-dimethylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2-fluoro-4,5-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(3-chloro-4,5-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2-chloro-3,4-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2,4-dimethoxy-6-methylbenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2,5-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2,3-dimethoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-(2-chloro-3-methoxybenzyl)-2-phenylcyclopropanamine;
(trans)-N-((lH-indol-5-yl)methyl)-2-phenylcyclopropanamine;
(trans)-2-(4-(benzyloxy)phenyl)-N-(pyridin-2-ylmethyl)cyclopropanamine;
(trans)-2-(4-(benzyloxy)phenyl)-N-(2-methoxybenzyl)cyclopropanamine;
(trans)-N-(l-(4-methoxyphenyl)ethyl)-2-phenylcyclopropanamine;
(trans)-N-(l -(3,4-dimethoxyphenyl)ethyl)-2-phenylcyclopropanamine;
(trans)-N-(l -(2,3-dihydrobenzo[b][l ,4]dioxin-6-yl)ethyl)-2- phenylcyclopropanamine;
(trans)-N-(l -(5-fluoro-2-methoxyphenyl)ethyl)-2-phenylcyclopropanamine;
(trans)-N-(l-(3,4-dimethoxyphenyl)propan-2-yl)-2-phenylcyclopropanamine;
(trans)-N-((3 -methyl- 1 , 2, 4-oxadiazol-5-yl)methyl)-2- phenylcyclopropanamine; and pharmaceutically acceptable salts thereof.
30. A pharmaceutical composition comprising a compound of any of claims 1-29 and a pharmaceutically acceptable carrier.
31 . Use of the compound of any of claims 1-29 for the manufacture of a medicament for treating or preventing cancer.
32. A method for treating or preventing a cancer comprising the administration of a therapeutically effective amount of a compound as defined in any of claims 1-29 or the pharmaceutical composition of claim 30 to a subject in need of such a treatment or prevention.
J J . The use of claim 31 or the method of claim 32 wherein said cancer is chosen from breast cancer, lung cancer, prostate cancer, testicular cancer, brain cancer, colorectal cancer, blood cancer, and skin cancer.
34. The method of claim 32 or 33 wherein said subject is a human.
35. A method of identifying LSDl selective and LSDl /MAO-B dual inhibitors comprising determining the ability of a test compound to inhibit LSDl , MAO-A, and MAO-B wherein a LSDl selective inhibitor is identified when the test compound inhibits LSDl to a greater extent than MAO-A and MAO-B and wherein a LSDl /MAO-B dual inhibitor is identified when the test compound inhibits LSDl and MAO-B to a greater extent than it inhibits MAO-A wherein said test compound is compound of Formula I:
Figure imgf000158_0001
FORMULA I wherein: each of R1 -R5 is independently chosen from -H, halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfinyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; R6 is chosen from -H and alkyl; R7 is chosen from -H, alkyl, and cycloalkyl;
R8 is a -L-heterocyclyl wherein the ring or ring system of said -L- heterocyclyl has from 0-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfmyl, sulfonyl, sulfonamide, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O- carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, and C-amido; or R8 is -L-aryl wherein the ring or ring system of said -L-aryl has from 1-3 substituents chosen from halo, alkyl, alkoxy, cycloalkoxy, haloalkyl, haloalkoxy, -L-aryl, -L-heterocyclyl, -L-carbocyclyl, acylamino, acyloxy, alkylthio, cycloalkylthio, alkynyl, amino, alkylamino, aryl, arylalkyl, arylalkenyl, arylalkynyl, arylalkoxy, aryloxy, arylthio, heteroarylthio, cyano, cyanato, haloaryl, hydroxyl, heteroaryloxy, heteroarylalkoxy, isocyanato, isothiocyanato, nitro, sulfmyl, sulfonyl, sulfonamido, thiocarbonyl, thiocyanato, trihalomethanesulfonamido, O-carbamyl, N-carbamyl, O- thiocarbamyl, N-thiocarbamyl, and C-amido; each L is independently chosen from -(CEb)n-(CH2),!-, -(CH2)nNH(CH2)n-, -(CHi)nO(CH2)I1-, and -(CHi)nS(CHi)n-, where each n is independently chosen from 0, 1 , 2, and 3 ; or a compound of Formula I where the phenyl ring attached to the cyclopropylamine moiety is replaced by an optionally substituted heteroaryl group and the other variables R6-R8 are as defined above.
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