WO2010055143A3 - Cell culture device and method of culturing cells - Google Patents

Cell culture device and method of culturing cells Download PDF

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Publication number
WO2010055143A3
WO2010055143A3 PCT/EP2009/065170 EP2009065170W WO2010055143A3 WO 2010055143 A3 WO2010055143 A3 WO 2010055143A3 EP 2009065170 W EP2009065170 W EP 2009065170W WO 2010055143 A3 WO2010055143 A3 WO 2010055143A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell culture
zone
cell
culturing cells
sensor element
Prior art date
Application number
PCT/EP2009/065170
Other languages
French (fr)
Other versions
WO2010055143A2 (en
Inventor
José CASTILLO
Jean-Christophe Drugmand
Original Assignee
Artelis S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40139871&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2010055143(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Artelis S.A. filed Critical Artelis S.A.
Priority to EP09760508A priority Critical patent/EP2356206B1/en
Priority to US13/129,163 priority patent/US8986979B2/en
Priority to EP12193161.2A priority patent/EP2568037B1/en
Publication of WO2010055143A2 publication Critical patent/WO2010055143A2/en
Publication of WO2010055143A3 publication Critical patent/WO2010055143A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C12M1/3407—Measure of electrical or magnetical factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a cell culture device (1) comprising a disposable culture vessel comprising at least one external wall made of a material having a Young modulus E of less than 50 GPa, in which there is situated at least one cell culture zone and at least one cell-free medium transfer zone, allowing a flow of a culture medium between the cell culture zone and the transfer zone, and at least one sensor element of a cell-density sensor wherein said sensor element is situated in the cell culture zone of said vessel. The sensor element is an electrode arrangement of at least two and preferably at least four measuring electrodes arranged on the inner side of an external wall of the disposable culture vessel and directed towards the cell culture zone. The present invention further relates to a method of culturing cells.
PCT/EP2009/065170 2008-11-13 2009-11-13 Cell culture device and method of culturing cells WO2010055143A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP09760508A EP2356206B1 (en) 2008-11-13 2009-11-13 Cell culture device and method of culturing cells
US13/129,163 US8986979B2 (en) 2008-11-13 2009-11-13 Cell culture device and method of culturing cells
EP12193161.2A EP2568037B1 (en) 2008-11-13 2009-11-13 Cell culture device and method of culturing cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0820779.7 2008-11-13
GBGB0820779.7A GB0820779D0 (en) 2008-11-13 2008-11-13 Cell culture device and method of culturing cells

Publications (2)

Publication Number Publication Date
WO2010055143A2 WO2010055143A2 (en) 2010-05-20
WO2010055143A3 true WO2010055143A3 (en) 2010-08-26

Family

ID=40139871

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/065170 WO2010055143A2 (en) 2008-11-13 2009-11-13 Cell culture device and method of culturing cells

Country Status (4)

Country Link
US (1) US8986979B2 (en)
EP (2) EP2356206B1 (en)
GB (2) GB0820779D0 (en)
WO (1) WO2010055143A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2479783A (en) * 2010-04-23 2011-10-26 Aber Instr Ltd A bioreactor with an impedance or biomass measuring probe.
EP2484750A1 (en) * 2011-02-07 2012-08-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Monitoring system for cell culture
EP2674480B2 (en) * 2012-06-15 2023-04-05 DASGIP Information and Process Technology GmbH Connection device for a sterile disposable fluid conduit of a disposable bioreactor and method for treating a fluid flow
US10717960B2 (en) 2011-10-10 2020-07-21 Dasgip Information And Technology Gmbh Biotechnological apparatus comprising a bioreactor, exhaust gas temperature control device for a bioreactor and a method for treating an exhaust gas stream in a biotechnological apparatus
KR20230156168A (en) 2015-08-08 2023-11-13 스토베 게엠베하 Disposable bioprocess system supporting biological activity
WO2018044699A1 (en) 2016-08-27 2018-03-08 3D Biotek, Llc Bioreactor
US10851350B1 (en) 2017-06-27 2020-12-01 Genelux Corporation Bioreactor production of virus from adherent cells
EP3728549A1 (en) 2017-12-20 2020-10-28 Univercells Technologies S.A. Bioreactor and related methods
WO2022038295A1 (en) * 2020-08-21 2022-02-24 Univercells Technologies S.A. Biomass measuring system for fixed bed bioreactor and related methods
CN116529382A (en) 2020-10-02 2023-08-01 吉恩勒克斯公司 Production of viruses from adherent cells in a bioreactor
CN114561292B (en) * 2022-04-27 2022-07-19 天津长和生物技术有限公司 Cell culture case with from drum-type carbon dioxide preparation facilities
CN115786120A (en) * 2022-11-01 2023-03-14 上海东富龙医疗装备有限公司 Disposable fixed bed for adherent cell culture

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0310677A (en) * 1989-06-09 1991-01-18 Shimadzu Corp Culture bag
WO2007039600A1 (en) * 2005-10-04 2007-04-12 Artelis Method of cell cultures and device for implementing it
US20070159920A1 (en) * 2006-01-11 2007-07-12 Sartorius Ag Container and method for the mixing of media
WO2007134267A2 (en) * 2006-05-13 2007-11-22 Advanced Technology Materials, Inc. Disposable bioreactor

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
US3504185A (en) * 1968-05-09 1970-03-31 Univ Syracuse Res Corp Apparatus for measuring and controlling cell population density in a liquid medium
US4267276A (en) * 1979-09-28 1981-05-12 Honeywell Inc. Medical specimen culture bottle
GB8516912D0 (en) 1985-07-04 1985-08-07 Johnson Matthey Plc Detecting growth of micro-organisms
JP3246664B2 (en) * 1993-01-29 2002-01-15 ニュー ブルンズウイック サイエンティフィック カンパニー インコーポレイテッド Scaffold and method for culturing suspended cells and apparatus therefor
JPH09313165A (en) * 1996-05-30 1997-12-09 Rikagaku Kenkyusho Widely using detector in bioreactor
DK1242220T3 (en) * 1999-12-20 2004-09-13 Polymer Sheet Applic Inc Process for making a composite material as well as composite material made therewith
FR2812725B1 (en) 2000-03-30 2002-10-18 Nanotec Solution DEVICE AND METHOD FOR DETERMINING THE CHARACTERISTICS OF BIOMASS
JP2003531373A (en) 2000-04-14 2003-10-21 ナノテック ソリューション Apparatus and method for characterizing biomass
US6673532B2 (en) * 2000-08-14 2004-01-06 University Of Maryland, Baltimore County Bioreactor and bioprocessing technique
US7470533B2 (en) * 2002-12-20 2008-12-30 Acea Biosciences Impedance based devices and methods for use in assays
US20050239198A1 (en) * 2004-04-27 2005-10-27 Baxter International, Inc. Stirred-tank reactor system
JP4680990B2 (en) 2004-06-04 2011-05-11 エクセラーエックス， インコーポレイテッド Disposable bioreactor system and method
US20060019376A1 (en) * 2004-07-21 2006-01-26 Bungay Henry R Iii Fermentation chamber and mixing apparatus
JP2008526203A (en) * 2004-12-29 2008-07-24 バイオジェン・アイデック・エムエイ・インコーポレイテッド Bioreactor process control system and method
WO2008005998A2 (en) 2006-07-07 2008-01-10 Georgia Tech Research Corporation Centimeter-scale, integrated diagnostics incubator for biological culturing
DE102006033326B4 (en) * 2006-07-19 2012-03-22 Holger Plettenberg culture vessel
US8008065B2 (en) 2006-08-02 2011-08-30 Finesse Solutions, Llc. Disposable bioreactor vessel port
FR2934371B1 (en) * 2008-07-25 2012-06-08 Nanotec Solution SINGLE-USE BIOMASS SENSOR DEVICE, METHOD OF MAKING SAME, AND SINGLE-USE BIOREACTOR INTEGRATING SENSOR

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0310677A (en) * 1989-06-09 1991-01-18 Shimadzu Corp Culture bag
WO2007039600A1 (en) * 2005-10-04 2007-04-12 Artelis Method of cell cultures and device for implementing it
US20070159920A1 (en) * 2006-01-11 2007-07-12 Sartorius Ag Container and method for the mixing of media
WO2007134267A2 (en) * 2006-05-13 2007-11-22 Advanced Technology Materials, Inc. Disposable bioreactor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 199109, Derwent World Patents Index; AN 1991-061846, XP002575823 *

Also Published As

Publication number Publication date
US20110223582A1 (en) 2011-09-15
EP2568037B1 (en) 2022-07-27
GB2465282A (en) 2010-05-19
GB0820779D0 (en) 2008-12-17
EP2568037A1 (en) 2013-03-13
US8986979B2 (en) 2015-03-24
WO2010055143A2 (en) 2010-05-20
EP2356206A2 (en) 2011-08-17
EP2356206B1 (en) 2012-12-26
GB0919936D0 (en) 2009-12-30

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