WO2010034798A1

WO2010034798A1 - 3h-imidazo [4, 5-c] pyridine-6-carboxamides as anti -inflammatory agents

Info

Publication number: WO2010034798A1
Application number: PCT/EP2009/062424
Authority: WO
Inventors: Roland Pfau; Kirsten Arndt; Henri Doods; Norbert Hauel; Klaus Klinder; Raimund Kuelzer
Original assignee: Boehringer Ingelheim International Gmbh
Priority date: 2008-09-25
Filing date: 2009-09-25
Publication date: 2010-04-01
Also published as: US8916599B2; CN102224143A; AR073403A1; RU2011116133A; MX2011002948A; US9040565B2; RU2011116131A; EP2361252A1; CN102164911A; TW201016682A; WO2010034797A1; UY32138A; AR073684A1; RU2011116129A; EP2350073B1; BRPI0919292A2; CN102164922A; IL211147A0; CN102164924A; AR073685A1

Abstract

There are provided compounds of formula (I), wherein R¹, R⁶, R⁸, Q², Q³, Q^3a, Q⁴, L and A have meanings given in the description, and pharmaceutically-acceptable salts thereof, which compounds are useful in the treatment of diseases in which inhibition of the activity of a member of the MAPEG family is desired and/or required, and particularly in the treatment of inflammation and/or cancer.

Description

3H-IMIDAZO [4 , 5-C] PYRIDINE-6-CARBOXAMIDES AS ANTI -INFLAMMATORY AGENTS

Field of the Invention

This invention relates to novel pharmaceutically-useful compounds, which compounds are useful as inhibitors of enzymes belonging to the membrane-associated proteins in the eicosanoid and glutathione metabolism (MAPEG) family. Members of the MAPEG family include the microsomal prostaglandin E synthase-1 (mPGES-1 ), 5-lipoxygenase-activating protein (FLAP), leukotriene C₄ synthase and microsomal glutathione S-transferases (MGST1 , MGST2 and MGST3). The compounds are of potential utility in the treatment of inflammatory diseases including respiratory diseases. The invention also relates to the use of such compounds as medicaments, to pharmaceutical compositions containing them, and to synthetic routes for their production.

Background of the Invention There are many diseases/disorders that are inflammatory in their nature. One of the major problems associated with existing treatments of inflammatory conditions is a lack of efficacy and/or the prevalence of side effects (real or perceived).

Inflammatory diseases that affect the population include asthma, inflammatory bowel disease, rheumatoid arthritis, osteoarthritis, rhinitis, conjunctivitis and dermatitis. Inflammation is also a common cause of pain. Inflammatory pain may arise for numerous reasons, such as infection, surgery or other trauma. Moreover, several diseases including malignancies and cardiovascular diseases are known to have inflammatory components adding to the symptomatology of the patients. Asthma is a disease of the airways that contains elements of both inflammation and bronchoconstriction. Treatment regimens for asthma are based on the severity of the condition. Mild cases are either untreated or are only treated with inhaled β-agonists which affect the bronchoconstriction element, whereas patients with more severe asthma typically are treated regularly with inhaled corticosteroids which to a large extent are anti-inflammatory in their nature. Another common disease of the airways with inflammatory and bronchoconstrictive components is chronic obstructive pulmonary disease (COPD). The disease is potentially lethal, and the morbidity and mortality from the condition is considerable. At present, there is no known pharmacological treatment capable of changing the course of the disease. The cyclooxygenase (COX) enzyme exists in two forms, one that is constitutively expressed in many cells and tissues (COX-1 ), and one that in most cells and tissues is induced by proinflammatory stimuli, such as cytokines, during an inflammatory response (COX-2). COXs metabolise arachidonic acid to the unstable intermediate prostaglandin H₂ (PGH₂). PGH₂ is further metabolized to other prostaglandins including PGE₂, PGF_2α, PGD₂, prostacyclin and thromboxane A₂. These arachidonic acid metabolites are known to have pronounced physiological and pathophysiological activity including pro-inflammatory effects. PGE₂ in particular is known to be a strong pro-inflammatory mediator, and is also known to induce fever and pain. Consequently, numerous drugs have been developed with a view to inhibiting the formation of PGE₂, including "NSAIDs" (non-steroidal antiinflammatory drugs) and "coxibs" (selective COX-2 inhibitors). These drugs act predominantly by inhibition of COX-1 and/or COX-2, thereby reducing the formation of PGE₂.

However, the inhibition of COXs has the disadvantage that it results in the reduction of the formation of all metabolites downstream of PGH₂, some of which are known to have beneficial properties. In view of this, drugs which act by inhibition of COXs are therefore known/suspected to cause adverse biological effects. For example, the non-selective inhibition of COXs by NSAIDs may give rise to gastrointestinal side-effects and affect platelet and renal function. Even the selective inhibition of COX-2 by coxibs, whilst reducing such gastrointestinal side-effects, is believed to give rise to cardiovascular problems. An alternative treatment of inflammatory diseases that does not give rise to the above- mentioned side effects would thus be of real benefit in the clinic. In particular, a drug that inhibits (preferably selectively) the transformation of PGH₂ to the pro-inflammatory mediator PGE₂ might be expected to reduce the inflammatory response in the absence of a corresponding reduction of the formation of other, beneficial arachidonic acid metabolites. Such inhibition would accordingly be expected to alleviate the undesirable side-effects mentioned above.

PGH₂ may be transformed to PGE₂ by prostaglandin E synthases (PGES). Two microsomal prostaglandin E synthases (mPGES-1 and mPGES-2), and one cytosolic prostaglandin E synthase (cPGES) have been described. The leukotrienes (LTs) are formed from arachidonic acid by a set of enzymes distinct from those in the COX / PGES pathway. Leukotriene B₄ is known to be a strong proinflammatory mediator, while the cysteinyl-containing leukotrienes C₄, D₄ and E₄ (CysLTs) are mainly very potent bronchoconstrictors and have thus been implicated in the pathobiology of asthma. The biological activities of the CysLTs are mediated through two receptors designated CySLT₁ and CysLT₂. As an alternative to steroids, leukotriene receptor antagonists (LTRas) have been developed in the treatment of asthma. These drugs may be given orally, but do not control inflammation satisfactorily. The presently used LTRas are highly selective for CySLT₁. It may be hypothesised that better control of asthma, and possibly also COPD, may be attained if the activity of both of the CysLT receptors could be reduced. This may be achieved by developing unselective LTRas, but also by inhibiting the activity of proteins, e.g. enzymes, involved in the synthesis of the CysLTs. Among these proteins, 5-lipoxygenase, 5- lipoxygenase-activating protein (FLAP), and leukotriene C₄ synthase may be mentioned. A FLAP inhibitor would also decrease the formation of the proinflammatory LTB₄. mPGES-1 , FLAP and leukotriene C₄ synthase belong to the membrane-associated proteins in the eicosanoid and glutathione metabolism (MAPEG) family. Other members of this family include the microsomal glutathione S-transferases (MGST1 , MGST2 and MGST3). For a review, c.f. P.-J. Jacobsson et al in Am. J. Respir. Crit. Care Med. 161 , S20 (2000). It is well known that compounds prepared as antagonists to one of the MAPEGs may also exhibit inhibitory activity towards other family members, c.f. J. H Hutchinson et al ^'m J. Med. Chem. 38, 4538 (1995) and D. Claveau et al in J. Immunol. 170, 4738 (2003). The former paper also describes that such compounds may also display notable cross-reactivity with proteins in the arachidonic acid cascade that do not belong to the MAPEG family, e.g. 5- lipoxygenase. Thus, agents that are capable of inhibiting the action of mPGES-1 , and thus reducing the formation of the specific arachidonic acid metabolite PGE₂, are likely to be of benefit in the treatment of inflammation. Further, agents that are capable of inhibiting the action of the proteins involved in the synthesis of the leukotrienes are also likely to be of benefit in the treatment of asthma and COPD. In addition to their anti-inflammatory effect, mPGES-1 inhibitiors are also known to be of potential use in treating or preventing a neoplasia, for example as decribed in international patent application WO 2007/124589. The rationale behind this may stem from the fact that the production of PGE2 is believed to promote the formation, growth and/or metastasis of neoplasias. As mPGES-1 is often expressed with COX-2 in benign and cancerous neoplasias, the inhibition of mPGES-1 (rather than COX-2) may cause the reduction of PGE2 and therefore mPGES-1 inhibitors may be useful the treatment of benign or malignant neoplasias.

The listing or discussion of an apparently prior-published document in this specification should not necessarily be taken as an acknowledgement that the document is part of the state of the art or is common general knowledge. The synthesis of various benzimidazoles has been disclosed by Carpenter ed a/ in the Journal of Combinatorial Chemistry (2006), 8(6), 907-914. However, no apparent medical use has been ascribed to such compounds.

Disclosure of the Invention

There is provided a compound of formula I,

in which

Q², Q³, Q^3a and Q⁴ respectively represent

or any one or two of Q , Q , Q and Q may alternatively and independently represent -N=;

R¹ represents halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14, -C(O)R^y15;

Ci-₇ alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl [which latter four groups are optionally substituted by one or more substituents selected from fluoro, -CN, N(R^y1)R^y2, -N(R^)-C(O )-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and -C(O)R^y15]; or heterocycloalkyl or heteroaryl (which latter two groups are optionally substituted by one or more substituents selected from R⁹); R², R³, R^3a and R⁴ independently represent hydrogen, halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14, -C(O)R^y15; Ci-7 alkyl, C₂-7 alkenyl, C₂-7 alkynyl, cycloalkyl [which latter four groups are optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and -C(O)R^y15] provided that if R³ or R^3a is a substituted Ci alkyl group, then the substituent cannot be -N(R^y5)-S(O)₂-R^y6; or any adjacent pair of R¹, R², R³, R^3a and R⁴ (i.e. R¹ and R^3a, R² and R³, R³ and R⁴ and R⁴ and R^3a) may be linked together to form, along with the essential carbon atoms of the Q² to Q⁴-containing ring to which they are necessarily attached, a further 5- to 7-membered ring, optionally containing one to three heteroatoms, which ring may contain one or two further unsaturations and which is optionally substituted by one or more Ci_3 alkyl and/or =0 substituents;

R⁶ represents hydrogen; heterocycloalkyl, aryl, heteroaryl (which latter three groups are optionally substituted by one or more substituents selected from R⁹); or Ci-6 alkyl, C_2-6 alkenyl, C_2-6 alkynyl, cycloalkyl, which latter four groups are optionally substituted by one or more substituents selected from fluoro, -

N(R^y1)R^y2, -N(R^)-C(O )-R^y4,

-N(R^y5)-S(O)₂-R^y6, -C(0)0R^y7, -C(O)N(R^y8)R^y9, -0R^y1°, -S(O)_m-R^y11, -

S(O)₂N(R^y13)R^y14, -C(0)R^y15, heterocycloalkyl, cycloalkyl, aryl and heteroaryl (which latter four groups are optionally substituted by one or more substituents selected from R⁹);

each R⁸ independently represents hydrogen, halo, -N(R^y1)R^y2, -0R^y1°,

-S(O)₂-R^y11; Ci_-6 alkyl, C_2-6 alkenyl, C_2-6 alkynyl, cycloalkyl, -0-Ci_-6 alkyl, -0-C_2-6 alkenyl,

-0-C_2-6 alkynyl, -O-cycloalkyl, -O-heterocycloalkyl [which latter nine groups are optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(0)0R^y7, -C(O)N(R^y8)R^y9, -0R^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14, -C(0)R^y15, cycloalkyl, heterocycloalkyl, aryl and heteroaryl (which latter four groups are optionally substituted by one or more substituents selected from R⁹)]; heterocycloalkyl or heteroaryl (which latter two groups are optionally substituted by one or more substituents selected from R⁹);

A represents aryl, heteroaryl, heterocycloalkyl, cycloalkyl, C_M2 alkyl, C₂-₁₂ alkenyl or C₂-₁₂ alkynyl, all of which are optionally substituted by one or more substituents selected from R⁹;

R⁹ represents, on each occasion when used herein: halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7,

-C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and

-C(O)R^y15;

Ci-7 alkyl, C₂-7 alkenyl, C₂-7 alkynyl, cycloalkyl, heterocycloalkyl [which latter five groups are optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7,

-C(O)R^y15]; or aryl or heteroaryl [which latter two groups are optionally substituted by one or more substituents selected from halo, -CN, Ci_-7 alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl (which latter four groups are optionally substituted by one or more substituents selected from fluoro and -OR^*2), -0-Ci_-7 alkyl, -0-C_2-7 alkenyl,

-0-C_2-7 alkynyl and -O-cycloalkyl (which latter four groups are optionally substituted by one or more fluoro atoms)]; or any two R⁹ substituents: when attached to the adjacent atoms of the A group; and, in the case where the R⁹ substituents are attached to a non-aromatic A group, when attached to the same atoms, may be linked together to form, together with the essential atoms of the A group to which the relevant R⁹ substituents are necessarily attached, a further 3- to 8-membered ring, optionally containing a further one or two heteroatoms, and which further ring optionally contains one or two unsaturations and which is optionally substituted by one or more Ci_-3 alkyl and/or =0 substituents;

m represents 0, 1 or 2; each R^y4, R*⁶, R^y11 and R^y15: independently represent Ci_-7 alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl, which latter four groups are optionally substituted by one or more fluoro atoms;

each R^*2, R^y1, R^y2, R*\ R^y5, R^y7, R^y8, R^y9, R^y1°, R^y12, R^y13 and R^y14: independently represent hydrogen or Ci_-7 alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl, heterocycloalkyl, which latter five groups are optionally substituted one or more substituents selected from fluoro and -OCi_3 alkyl; or any two groups, when attached to the same nitrogen atom (i.e. R^y1 and R*², R^y8 and R*⁹, and R^y13 and R^y14), may, together with that nitrogen atom to which they are necessarily attached, be linked together to form a 3- to 8-membered ring, optionally containing one or two further heteroatoms and which ring optionally contains one or two unsaturations and is optionally substituted by one or more Ci_-3 alkyl and/or =0 substituents,

or a pharmaceutically acceptable salt thereof,

which compounds are hereinafter referred to as 'the compounds of the invention'.

Pharmaceutically-acceptable salts include acid addition salts and base addition salts. Such salts may be formed by conventional means, for example by reaction of a free acid or a free base form of a compound of formula I with one or more equivalents of an appropriate acid or base, optionally in a solvent, or in a medium in which the salt is insoluble, followed by removal of said solvent, or said medium, using standard techniques (e.g. in vacuo, by freeze- drying or by filtration). Salts may also be prepared by exchanging a counter-ion of a compound of the invention in the form of a salt with another counter-ion, for example using a suitable ion exchange resin.

Compounds of the invention may contain double bonds and may thus exist as E (entgegen) and Z (zusammen) geometric isomers about each individual double bond. All such isomers and mixtures thereof are included within the scope of the invention.

Compounds of the invention may also exhibit tautomerism. All tautomeric forms and mixtures thereof are included within the scope of the invention. For instance, a compound containing the moiety "1H-benzimidazole" may be considered to be identical to a corresponding compound containing a "3H-benzimidazole" moiety. Compounds of the invention may also contain one or more asymmetric carbon atoms and may therefore exhibit optical and/or diastereoisomerism. Diastereoisomers may be separated using conventional techniques, e.g. chromatography or fractional crystallisation. The various stereoisomers may be isolated by separation of a racemic or other mixture of the compounds using conventional, e.g. fractional crystallisation or HPLC, techniques. Alternatively the desired optical isomers may be made by reaction of the appropriate optically active starting materials under conditions which will not cause racemisation or epimerisation (i.e. a 'chiral pool' method), by reaction of the appropriate starting material with a 'chiral auxiliary' which can subsequently be removed at a suitable stage, by derivatisation (i.e. a resolution, including a dynamic resolution), for example with a homochiral acid followed by separation of the diastereomeric derivatives by conventional means such as chromatography, or by reaction with an appropriate chiral reagent or chiral catalyst all under conditions known to the skilled person. All stereoisomers and mixtures thereof are included within the scope of the invention. Unless otherwise specified, Ci_-q alkyl, and Ci_-q alkylene, groups (where q is the upper limit of the range), defined herein may be straight-chain or, when there is a sufficient number (i.e. a minimum of two or three, as appropriate) of carbon atoms, be branched-chain. For the avoidance of doubt, such groups are fully saturated. Unless otherwise specified, C₂-_q alkenyl, and C₂-_q alkenylene, groups (where q is the upper limit of the range) refer to a hydrocarbon chain (in the case of alkenylene, the chain links two moieities) containing one or more double bond. Such groups as defined herein may be straight-chain or, when there is a sufficient number (i.e. a minimum of two or three, as appropriate) of carbon atoms, be branched-chain. Unless otherwise specified, C₂-_q alkynyl, and C₂-_q alkynylene, groups (where q is the upper limit of the range) refer to a hydrocarbon chain (in the case of alkynylene, the chain links two moieities) containing one or more triple bond. Such groups as defined herein may be straight-chain or, when there is a sufficient number (i.e. a minimum of three or four, as appropriate) of carbon atoms, be branched-chain. In the instance where a 'cycloalkyl' group (e.g. C_3-q cycloalkyl) is specifically mentioned, such groups may be monocyclic or bicyclic non-aromatic alkyl groups, which may further be bridged (so forming, for example, fused ring systems). Such cycloalkyl groups may be saturated or unsaturated, e.g. containing one or more double bond (forming for example a C_5-C, cycloalkenyl). Optional substituents may be attached at any point on the cycloalkyl group. Cycloalkyl groups that may be mentioned preferably include C_3--_I2 cycloalkyl, for instance a 3- to 7-membered monocyclic cycloalkyl group, a C_7-n (e.g. C_8-n) bicyclic cycloalkyl group or a C_8-i₂ (e.g. C₉-n) tricyclic cycloalkyl group. As stated above, cycloalkyl groups may further be bridged, so forming, for example, an adamantyl group (for example when a bicyclic cycloalkyl group is bridged). The term 'acyclic' alkyl group when used herein refers to an alkyl group that is not cyclic, but may be branched-chain or, is preferably, straight-chain.

For the avoidance of doubt, the term "bicyclic", when employed in the context of cycloalkyl, refers to such groups in which the second ring is formed between two adjacent atoms of the first ring (i.e. systems of two rings share one bond formed with two adjacent carbon atoms). The term "bridged", when employed in the context of cycloalkyl groups refers to monocyclic or bicyclic groups in which two non-adjacent atoms are linked by an alkylene chain. The term "halo", when used herein, includes fluoro, chloro, bromo and iodo. Aryl groups that may be mentioned include C_6--_H (e.g. C_6-io) aryl groups. Such groups may be monocyclic, bicyclic or tricyclic and have between 6 and 14 ring carbon atoms, in which at least one ring is aromatic. C&.u aryl groups include phenyl, naphthyl and the like, such as 1 ,2,3,4-tetrahydronaphthyl, indanyl, indenyl and fluorenyl. The point of attachment of aryl groups may be via any atom of the ring system, for instance when aryl groups are bicyclic or tricyclic, they are linked to the rest of the molecule via an atom of an aromatic or non- aromatic ring. Heteroaryl groups that may be mentioned include those which have between 5 and 14 (e.g. 10) members. Such groups may be monocyclic, bicyclic or tricyclic, provided that at least one of the rings is aromatic and wherein at least one (e.g. one to four) of the atoms in the ring system is other than carbon (i.e. a heteroatom). Heteroaryl groups that may be mentioned include acridinyl, benzimidazolyl, benzodioxanyl, benzodioxepinyl, benzodioxolyl (including 1 ,3-benzodioxolyl), benzofuranyl, benzofurazanyl, benzothiazolyl, benzoxadiazolyl (including 2,1 ,3-benzoxadiazolyl), benzoxazinyl (including 3,4-dihydro-2/-/-1 ,4-benzoxazinyl), benzoxazolyl, benzomorpholinyl, benzoselenadiazolyl (including 2,1 ,3-benzoselenadiazolyl), benzothiadiazolyl (including 2,1 ,3-benzothiadiazolyl), benzothienyl, carbazolyl, chromanyl, cinnolinyl, furanyl, imidazolyl, imidazopyridyl (including imidazo[4,5-b]pyridyl, imidazo[5,4-b]- pyridyl and imidazo[1 ,2-a]pyridyl), indazolyl, indolinyl, indolyl, isobenzofuranyl, isochromanyl, isoindolinyl, isoindolyl, isoquinolinyl, isothiaziolyl, isothiochromanyl, isoxazolyl, naphthyridinyl (including 1 ,6-naphthyridinyl or, preferably, 1 ,5-naphthyridinyl and 1 ,8-naphthyridinyl), oxadi- azolyl (including 1 ,3,4-oxadiazolyl), oxazolyl, phenazinyl, phenothiazinyl, phthalazinyl, pteridinyl, purinyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridyl, pyrimidinyl, pyrrolyl, quinazolinyl, quinolinyl, quinolizinyl, quinoxalinyl, tetrahydroisoquinolinyl (including 1 ,2,3,4-tetrahydro- isoquinolinyl and 5,6,7,8-tetrahydroisoquinolinyl), tetrahydroquinolinyl (including 1 ,2,3,4- tetrahydroquinolinyl and 5,6,7,8-tetrahydroquinolinyl), tetrazolyl, thiadiazolyl (including 1 ,3,4-thiadiazolyl), thiazolyl, oxazolopyridyl (including oxazolo[4,5-b]pyridyl, oxazolo[5,4- b]pyridyl and, in particular, oxazolo[4,5-c]pyridyl and oxazolo[5,4-c]pyridyl), thiazolopyridyl (including thiazolo[4,5-b]pyridyl, thiazolo[5,4-b]pyridyl and, in particular, thiazolo[4,5-c]pyridyl and thiazolo[5,4-c]pyridyl), thiochromanyl, thienyl, triazolyl (including 1 ,2,3-triazolyl and 1 ,2,4-triazolyl) and the like. Substituents on heteroaryl groups may, where appropriate, be located on any atom in the ring system including a heteroatom. The point of attachment of heteroaryl groups may be via any atom in the ring system including (where appropriate) a heteroatom (such as a nitrogen atom), or an atom on any fused carbocyclic ring that may be present as part of the ring system. When heteroaryl groups are bicyclic or tricyclic, they may be linked to the rest of the molecule via an atom of an aromatic or non-aromatic ring. Heteroaryl groups may also be in the N- or S- oxidised form (so forming, for example, a pyridine Λ/-oxide).

Heterocycloalkyl groups that may be mentioned include non-aromatic monocyclic and bicyclic heterocycloalkyl groups (which groups may further be bridged) in which at least one (e.g. one to four) of the atoms in the ring system is other than carbon (i.e. a heteroatom), and in which the total number of atoms in the ring system is between three and twelve (e.g. between five and ten). Further, such heterocycloalkyl groups may be saturated or unsaturated containing one or more double and/or triple bonds, forming for example a C₂-_q heterocycloalkenyl (where q is the upper limit of the range) or a C_7-q heterocycloalkynyl group. C_2-q heterocycloalkyl groups that may be mentioned include 7-azabicyclo- [2.2.1]heptanyl, 6-azabicyclo[3.1.1]heptanyl, 6-azabicyclo[3.2.1]-octanyl, 8- azabicyclo[3.2.1]octanyl, aziridinyl, azetidinyl, dihydropyranyl, dihydropyridyl, dihydropyrrolyl (including 2, 5-dihydropyrrolyl), dioxolanyl (including 1 ,3-dioxolanyl), dioxanyl (including 1 ,3- dioxanyl and 1 ,4-dioxanyl), dithianyl (including 1 ,4-dithianyl), dithiolanyl (including 1 ,3- dithiolanyl), imidazolidinyl, imidazolinyl, morpholinyl, 7-oxabicyclo[2.2.1]heptanyl, 6- oxabicyclo[3.2.1]-octanyl, oxetanyl, oxiranyl, piperazinyl, piperidinyl, pyranyl, pyrazolidinyl, pyrrolidinonyl, pyrrolidinyl, pyrrolinyl, quinuclidinyl, sulfolanyl, 3-sulfolenyl, tetrahydropyranyl, tetrahydrofuranyl, tetrahydropyridyl (such as 1 ,2,3,4-tetrahydropyridyl and 1 ,2,3,6- tetrahydropyridyl), thietanyl, thiiranyl, thiolanyl, thiomorpholinyl, trithianyl (including 1 ,3,5- trithianyl), tropanyl and the like. Substituents on heterocycloalkyl groups may, where appropriate, be located on any atom in the ring system including a heteroatom. Further, in the case where the substituent is another cyclic compound, then the cyclic compound may be attached through a single atom on the heterocycloalkyl group, forming a so-called "spiro"-compound. The point of attachment of heterocycloalkyl groups may be via any atom in the ring system including (where appropriate) a heteroatom (such as a nitrogen atom), or an atom on any fused carbocyclic ring that may be present as part of the ring system. Heterocycloalkyl groups may also be in the N- or S- oxidised form. Heteroatoms that may be mentioned include phosphorus, silicon, boron, tellurium, selenium and, preferably, oxygen, nitrogen and sulfur. For the avoidance of doubt, in cases in which the identity of two or more substituents in a compound of formula I may be the same, the actual identities of the respective substituents are not in any way interdependent. For example, in case of R⁸, the respective -C(R⁸)- groups in question may be the same or different. Similarly, when groups are substituted by more than one substituent as defined herein, the identities of those individual substituents are not to be regarded as being interdependent. For example, when an A group is substituted by two R⁹ substituents, in which, in both cases, R⁹ represents Ci_-7 alkyl substituted by -

N(R^y1)R^y2, then the identities of the two -N(R^y1)R^y2 groups are not to be regarded as being interdependent, i.e. the two -N(R^y1)R^y2 moieties may be the same or different, i.e. at each occurrence, R^y1 and R^ may also be the same or different. For the avoidance of doubt, when a term such as "R^y1 to R^y15" is employed herein, this will be understood by the skilled person to mean R^y1, R*², R^y3, R^y4, R^y5, R^y6, R^y7, R^y8, R^y9, R^y1°, R^y11, R^y12, R^y13, R¹⁴ and R^y15 inclusively. Further, when a term such as "R¹ to R⁵" is employed herein, the skilled person will understand this to mean R¹, R², R³, R^3a, R⁴ and R⁵ inclusively. Similarly, when the term "Q² to Q⁴" is employed, this will be understood to mean Q², Q³, Q^3a and Q⁴ inclusively. For the avoidance of doubt, when the compound of formula I is substituted by a heterocycloalkyl or heteroaryl group, for example when R¹ or R⁸ represent such substituents, then the point of attachment may be via a carbon atom or heteroatom (e.g. nitrogen heteroatom), assuming that the valency of the heteroatom permits. Similarly, when heterocycloalkyl or heteroaryl groups are substituted with further substituents, then those substituents may be attached at any position including on a carbon atom or heteroatom (e.g. a nitrogen heteroatom), again assuming that the valency permits.

For the avoidance of doubt, where it is mentioned herein that alkyl, alkenyl, alkynyl or cycloalkyl groups may be substituted with one or more halo atoms, then those halo atoms are preferably fluoro atoms. The skilled person will appreciate that there may be free rotation around the nitrogen-carbon bond to which the requisite phenyl ring bearing the R¹ to R⁴ substituents is pending. In view of this (when Q² to Q⁴ respectively represent -C(R²)=, -C(R³)=, -C(R^3a)= and -C(R⁴)=), the R¹ and R² positions are 'identical' (as are the R³ and R^3a positions) relative to the point of attachment of that phenyl ring. Hence, the definitions of R¹ and R² may be interchanged (in which case the definitions of R³ and R^3a are also 'interchanged', relative to the definitions of R¹ and R²), in view of the fact that both R¹ and R² represent ortho phenyl substituents. The important aspect in relation to the R¹ to R⁴ substituents is therefore their positions relative to one another, rather than their positions relative to the point of attachment of that phenyl ring to the rest of the compound of formula I. For the avoidance of doubt, when preferred features are mentioned herein, then such features may be taken independently of others preferred features or conjunctively with other preferred features. The skilled person will appreciate that compounds of formula I that are the subject of this invention include those that are stable. That is, compounds of the invention include those that are sufficiently robust to survive isolation from e.g. a reaction mixture to a useful degree of purity.

In one embodiment, the invention provides compounds of formula I as described above and in which

Q² represents -C(R²)=; and

Q³, Q^3a and Q⁴ respectively represent -C(R³)=, -C(R^3a)= and -C(R⁴)=; or any one Q³, Q^3a and Q⁴ may alternatively and independently represent -N=;

In another embodiment, the invention provides compounds of formula I according to any of the preceding embodiments and in which

Q², Q³ and Q^3a respectively represent -C(R²)=, -C(R³)= and

-C(R^3a)=; and Q⁴ represents -N=. In another embodiment, the invention provides compounds of formula I according to any of the preceding embodiments and in which

Q², Q^3a and Q⁴ respectively represent -C(R²)=, -C(R^3a)= and -C(R⁴)=; and

Q³ represents -N=;

or

Q², Q³ and Q⁴ respectively represent -C(R²)=, -C(R³)= and -C(R⁴)=; and

Q^3a represents -N=.

R¹ represents Ci_-4 alkyl (optionally substituted by one or more fluoro atoms), C_3-6 cycloalkyl or halo.

R¹ represents Ci_-3 alkyl (optionally substituted by one or more fluoro atoms), C_3-6 cycloalkyl or halo.

R² represents hydrogen, Ci_-4 alkyl [optionally substituted by one or more atoms selected from fluoro, -CN, -N(R^)R*², -N(R⁵^)-C(O)-R* -N(R^y5)-S(O)₂-R^y6, -

C(O)OR^y7, -C(O)N(R^y8)R^y9,-OR^y10, -S(O)_m-R^y11, -S(O)₂O-R^y12, S(O)₂N(R^y13)R^y14 and -C(O)R^y15], C_3-6 cycloalkyl, halo Or -O-Ci_-3 alkyl (optionally substituted by one or more fluoro atoms). In another embodiment, the invention provides compounds of formula I according to any of the preceding embodiments and in which

R² represents hydrogen, Ci_-3 alkyl [optionally substituted by one or more atoms selected from fluoro, -CN, -N(R^)R*², -N(R^)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, - C(O)OR^y7, -C(O)N(R^y8)R^y9,-OR^y10, -S(O)_m-R^y11, -S(O)₂O-R^y12, S(O)₂N(R^y13)R^y14 and -C(O)R^y15], C_3-6 cycloalkyl, halo Or -O-Ci_-3 alkyl (optionally substituted by one or more fluoro atoms).

In another embodiment, the invention provides compounds of formula I according to any of the preceding embodiments and in which R³, R^3a and R⁴ independently represent hydrogen, Ci_-3 alkyl (optionally substituted by one or more fluoro atoms) or halo.

R⁹ represents halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6,

-C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and/or -C(O)R^y15; or

C-_I-7 alkyl optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6,

-C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and/or -C(O)R^y15; or aryl, heteroaryl (which latter two groups are optionally substituted by one or more substituents selected from -0-Ci_-3 alkyl, -CN, halo and Ci_-2 alkyl optionally substituted by one or more fluoro atoms); or any two R⁹ groups may be linked together as defined above. In another embodiment, the invention provides compounds of formula I according to any of the preceding embodiments and in which

R⁸ represents hydrogen, halo, Ci_-3 alkyl [optionally substituted by one or more substituents selected from fluoro, -OR^y1°, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, and

-C(O)N(R^y8)R^y9] or -OR^y10.

R⁶ represents hydrogen or Ci_-6 alkyl optionally substituted by one or more substituents selected from -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)₂R^y11 and a 4- to 6-membered heterocycloalkyl group (containing two or one heteroatom(s) selected from oxygen and nitrogen).

R^*2, R^y1, R^y2, R*³, R^y5, R^y7, R^y8, R*⁹, R^y1°, R^y12, R^y13 and R^y14 independently represent hydrogen or Ci_-4 alkyl optionally substituted by one or more fluoro atoms or -OCi_-2 alkyl groups; or any pair of R^y1 and R*², R^y8 and R^ and/or R^y13 and R^y14 are linked together to form a 3- to 7-membered ring, optionally containing one further nitrogen or oxygen heteroatom, one or two further double bonds, and which ring is optionally substituted by one or more Ci_-2 alkyl or =0 substituents.

R^y4, R^, R^y11 and R^y15 independently represent Ci_-4 alkyl.

In another embodiment, the invention provides compounds of formula I according to any of the preceding embodiments and in which A represents C_M2 linear or branched alkyl, aryl, heteroaryl, 5- or 6-membered heterocycloalkyl; or C_3-io cycloalkyl, all of which groups are optionally substituted by one or more substituents selected from R⁹.

A represents C_4--_I2 linear or branched alkyl, aryl, heteroaryl, 5- or 6-membered heterocycloalkyl; or C_3-7 cycloalkyl (all of which groups are optionally substituted by one or more substituents selected from R⁹); or arylmethylene, heteroarylmethylene [which latter two groups are optionally substituted by one or more groups selected from Ci_-7 alkyl (optionally substituted by one or more substituents selected from fluoro and -OR^*2), halo, -CN and/or -0-Ci_-7 alkyl (optionally substituted by one or more fluoro atoms)].

In another embodiment, the invention provides compounds according to any of the preceding embodiments, namely compounds of formula Ia, Ib, Ic or Id

Ib

in which R¹ represents Ci_-3 alkyl (optionally substituted by one or more fluoro atoms), C_3-6 cycloalkyl, fluoro, chloro, bromo;

R² represents hydrogen, Ci_-3 alkyl (optionally substituted by one or more fluoro atoms), C_3-6 cycloalkyl, fluoro, chloro, bromo;

R ₃3 , I R~I3B and R independently represent hydrogen, fluoro, chloro, bromo, Ci_-3 alkyl (optionally substituted by one or more fluoro atoms);

represents hydrogen or Ci_-6 alkyl optionally substituted by one or more substituents selected from fluoro, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂- R*⁶, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)₂R^y11 and a 4- to 6-membered heterocycloalkyl group (containing two or one heteroatom(s) selected from oxygen and nitrogen);

independently represents hydrogen, fluoro, chloro, bromo, -OR^y1° or Ci_-3 alkyl (optionally substituted by one or more fluoro atoms); A represents phenyl, pyridyl, 5- or 6-membered heterocycloalkyl, C_3-7 cycloalkyl,

C_5-8 linear or branched alkyl (all of which groups are optionally substituted by one or more substituents selected from R⁹), or benzyl, pyridylmethylene [which latter two groups are optionally substituted by one or more groups selected from Ci_-7 alkyl (optionally substituted by one or more substituents selected from fluoro and -OR^*2), halo, -CN and/or -0-Ci_-7 alkyl (optionally substituted by one or more fluoro atoms)];

R⁹ represents on each occasion when used herein: halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°,

-S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and/or -C(O)R^y15, Ci_-6 alkyl optionally substituted by one or more substituents selected from halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and/or -C(O)R^y15; or

any two R⁹ substituents, when attached to the adjacent atoms of the A group and, in the case where the R⁹ substituents are attached to a non-aromatic A group, when attached to the same atoms, may be linked together to form, together with the essential atoms of the A group to which the relevant R⁹ substituents are necessarily attached, a further 3- to 8-membered ring, optionally containing a further one or two heteroatoms, and which further ring optionally contains one or two unsaturations and which is optionally substituted by one or more Ci_-3 alkyl and/or =0 substituents; and the substituents R^*2, R^y1, R*², R^y3, R^y4, R^y5, R^y6, R^y7, R^y8, R^y9, R^y1°, R^y11, R^y12, R^y13, R^y14 and

R^y15 have the meaning as defined in the embodiments above.

In another embodiment, the invention provides compounds according to any of the preceding embodiments, namely compounds of formula Ie, If, Ig or Ih

in which R¹ represents chloro, bromo, fluoro, Ci_-3 alkyl (which latter alkyl group is optionally substituted by one or more fluoro-atoms);

R² represents hydrogen, chloro, bromo, fluoro, Ci_3- alkyl (which latter alkyl group is optionally substituted by one or more fluoro atoms);

R³, R^3a and R⁴ independently represent hydrogen, chloro, bromo, fluoro, Ci_-3- alkyl (which latter alkyl group is optionally substituted by one or more fluoro atoms);

R⁶ represents hydrogen; Ci_-4 alkyl optionally substituted by one or more fluoro atoms;

A represents phenyl, 2-pyridyl, 5- or 6-membered heterocycloalkyl, C3-7 cycloalkyl, C_5-8 linear or branched alkyl (all of which groups are optionally substituted by one or more substituents selected from R⁹); or benzyl, pyridin-2-yl-methylene [which latter two groups are optionally substituted by one or more groups selected from Ci_-7 alkyl (optionally substituted by one or more substituents selected from fluoro and -OR^*2), halo, -CN and/or -0-Ci_-7 alkyl (optionally substituted by one or more fluoro atoms)];

R⁹ represents halo, -0-Ci_-4 alkyl, Ci_-4 alkyl, C_3-5 cycloalkyl, (which latter three groups are optionally substituted by one or more fluoro atoms.

In a further embodiment, the invention provides compounds namely those of the examples described hereinafter.

Compounds of the invention may be made in accordance with techniques that are well known to those skilled in the art, for example as described hereinafter.

According to a further aspect of the invention there is provided a process for the preparation of a compound of formula I, which process comprises:

(i) for compounds of formula I, reaction of a compound of formula II,

wherein: in each case, R ->6 , ι R->8 and A are as hereinbefore defined, with a compound of formula

wherein R¹, Q², Q³, Q^3a and Q⁴ are as hereinbefore defined, under standard conditions known to those skilled in the art, for example in the presence of a suitable solvent (such as diethyl ether, or, preferably, dimethylformamide, dichloromethane, acetononitrile and/or tetrahydrofuran) and preferably in the presence of a suitable 'coupling' reagent (which reagent is preferably added during the course of the reaction, e.g. when there is no more starting material present and/or a thiourea intermediate has been formed) that may enhance the reactivity of any intermediate that may be formed (e.g. a thiourea intermediate such of formulae INA, 1MB, NIC and/or HID described hereinafter) between the reaction of the compound of formula Il with the compound of formula III, for instance a carbodiimide based compound such as dicyclohexylcarbodiimide (DCC), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (or salt, e.g. hydrochloride, thereof) or, preferably Λ/,Λ/-diisopropylcarbodiimide (DIC), which reaction may proceed at any suitable temperature (e.g. one between about O⁰C to about 200⁰C), and which may also be performed in the presence of an additive (such as 2,2,2-trifluoro-Λ/,O-bis-(trimethylsilyl)-acetamide). Alternatively, this reaction may be performed in the presence of a suitable base or mixture of bases, such as those described hereinafter (process step (N)), for example by reaction in the presence of triethylamine and/or DMAP (optionally in the presence of a suitable solvent such as dichloromethane), after which any intermediate so formed may be protected, optionally isolated and reacted in the presence of an aqueous basic solution (e.g. aqueous NaOH; optionally mixed with a further suitable solvent such as an alcoholic solvent), which reaction may take place at ambient temperature or up to reflux. The skilled person will appreciate that the reaction between compounds of formulae Il and III may proceed via intermediates of formulae INA or 1MB (as appropriate),

MIA MIB wherein R¹ ,R⁶, R⁸, Q², Q³, Q^3a, Q⁴ and A are as hereinbefore defined. Such intermediates may be isolated or may be produced in situ in the reaction to form a compound of formula I. When such intermediates are produced separately, then they may be reacted in the presence of solvent (e.g. acetonitrile and/or methanol) and that the intermediate so formed may be then reacted under the conditions set out above;

(ii) for compounds of formula I , reaction of a compound of formula IV,

or a derivative thereof (e.g. an ester derivative, such as a methyl ester), wherein R¹, Q², Q³, Q^3a, Q⁴, R⁶ and R⁸ are as hereinbefore defined, with a compound of formula V,

A-NH, V wherein A is as hereinbefore defined, under coupling reaction conditions, for example at around room temperature or above (e.g. up to 40-18O⁰C), optionally in the presence of a suitable base (e.g. sodium hydride, sodium bicarbonate, potassium carbonate, pyrrolidino- pyridine, pyridine, triethylamine, tributylamine, trimethylamine, dimethylaminopyridine, diisopropylamine, 1 ,8-diazabicyclo[5.4.0]undec-7-ene, sodium hydroxide, Λ/-ethyldiiso- propylamine, Λ/-(methylpolystyrene)-4-(methylamino)pyridine, butyllithium (e.g. n-, s- or t- butyllithium) or mixtures thereof), an appropriate solvent (e.g. tetrahydrofuran, pyridine, toluene, dichloromethane, chloroform, acetonitrile, dimethylformamide, dimethylsulfoxide, trifluoromethylbenzene, triethylamine or water) and a suitable coupling agent (e.g. 1 ,1 '- carbonyldiimidazole, Λ^/V-dicyclohexylcarbodiimide, Λ/,Λ/-diisopropylcarbodiimide, 1-(3- dimethylamino-propyl)-3-ethylcarbodiimide (or salt, e.g. hydrochloride thereof), N, N'- disuccinimidyl carbonate, benzotriazol-i-yloxytris(dimethylamino) phosphonium hexafluoro- phosphate, 2-(1 /-/-benzotriazol-1-yl)-1 ,1 ,3,3-tetramethyluronium hexafluorophosphate, benzotriazol-1 -yloxytrispyrrolidinophosphonium hexafluorophosphate, bromo-tris-pyrrolidino- phosponium hexafluorophosphate, 2-(1 /-/-benzotriazol-1 -yl)-1 ,1 , 3, 3-tetramethyluronium tetrafluorocarbonate, i-cyclohexyl-carbodiimide-3-propyloxymethyl polystyrene, O-(7-aza- benzotriazol-1-yl)-N,N,N',N'-tetra-methyluronium hexafluorophosphate, O-pentafluorophenyl- N,N,N',N'-tetra-methyluronium hexafluorophosphate, O-(benzotriazol-1-yl)-N,N,N',N'-tetra- methyluronium tetrafluoroborate or mixtures thereof). Alternatively, compounds of formula III may first be activated by treatment with a suitable reagent (e.g. oxalyl chloride, thionyl chloride, phosphorous pentachloride, phosphorous oxychloride, (1-chloro-2-methyl-propen- yl)-dimethyl-amine or the like, or mixtures thereof) optionally in the presence of an appropriate solvent (e.g. dichloromethane, THF, toluene or benzene) and a suitable catalyst (e.g. DMF), resulting in the formation of the respective acyl chloride. This activated intermediate may then be reacted with a compound of formula V under standard conditions, such as those described above. An alternative way of performing this step, includes the reaction of an ester derivative of a compound of formula IV (e.g. an ethyl or, preferably, a methyl ester) with a compound of formula V, in the presence of, e.g. trimethylaluminium, optionally in the presence of a suitable solvent (e.g. dichloromethane or tetrahydrofuran) under an inert atmosphere; Compounds of formula Il may be prepared by reduction of a compound of formula XX,

wherein Y^1a represents -NO₂ (or an azido group), and X^1a represents -N(R⁶)H or, in the case where the compound of formula Il to be formed is one in which both X¹ and Y¹ represent -N(H)-, then both of X^1a and Y^1a may represent -NO₂ (or an azido group), and R⁸ and A are as hereinbefore defined, under standard conditions known to those skilled in the art, for example, under hydrogenation reaction conditions, including catalytic hydrogenation reaction conditions (e.g. employing a precious metal catalyst such as a platinum group catalyst, e.g. platinum or, preferably, palladium, which latter may be employed as 10%-20% Pd/C, or employing a non-precious metal catalyst such as one based on nickel, e.g. Raney nickel), for example in the presence of a suitable solvent such as diethyl ether or, preferably, ethyl acetate, tetrahydrofuran or an alcoholic solvent (e.g. EtOH or MeOH), or mixtures thereof. Alternatively, the reduction may be performed in the presence of other suitable conditions, such as employing a mixture of Sn/HCI or Fe powder in EtOH and/or acetic acid and NH₄CI.

Compounds of formula INA and 1MB (the latter with R⁶ = H) may be prepared by reduction of a corresponding compound of formula XXA or XXB,

XXA XXB wherein R¹, R⁶, R⁸, Q², Q³, Q^3a, Q⁴ and A are as hereinbefore defined, under reduction reaction conditions for example such as those hereinbefore described in respect of preparation of compounds of formula II. The skilled person will appreciate that a similar reaction may be performed on compounds in which the nitro group is replaced with an azido group.

Compounds of formula XX may be prepared by nitration of a compound of formula XXIII,

XXIII

wherein X^1b represents -N(R⁶)H and Y^1b represents hydrogen, or X^1b represents hydrogen and Y^1b represents -NH₂, and R⁸ and A are as hereinbefore defined, under standard nitration reaction conditions, for example in the presence of a mixture of nitric acid and sulfuric acid (e.g. cone, sulfuric acid) which may be mixed at low temperatures (e.g. at about O⁰C), thereby forming a nitronium ion in situ, which may then react with the compound of formula XXIII.

Alternatively, compounds of formula XX in which one of X^1a and Y^1a represents -NO₂ and the other represents -NH₂ or -N(R⁶)H may be prepared by reaction of a compound of formula XXIIIA,

XXIIIA

wherein one of X^1b1 and Y^1b1 represents -NO₂ and the other represents a suitable leaving group, such as hereinbefore defined in respect of L^yb (and preferably represents a halo group, such as chloro), and A and R⁸ are as hereinbefore defined, with either: ammonia (or a suitable source thereof; for example, methanolic ammonia, or the like); or, for the introduction of the appropriate -N(R⁶)H (e.g when R⁶ is hydrogen), the corresponding amine R⁶-NH₂, under standard nucleophilic aromatic substitution reaction conditions.

Compounds of formula XXA and XXB in which X¹ and Y¹ preferably represent -N(H)- may be prepared by reaction of a compound of formula XXIIIB or XXIIIC,

XXlIlB XXlIlC

respectively, wherein R⁸ and A are as hereinbefore defined, with a compound of formula X as hereinbefore defined, under standard reaction conditions, for example such as those hereinbefore described in respect of preparation of compounds of formula I (process step (iv) above).

Compounds of formulae III, INA, 1MB, IV, V, Vl, VII, VIII, X, Xl, XII, XIII, XIV, XV, XVII, XVIII, XIX, XXIIB, XXIIC, XXIII, XXIIIB, XXIIIC, XXIIIA, XXV, XXVI, XXVIA, XXVII and XXVIII are either commercially available, are known in the literature, or may be obtained either by analogy with the processes described herein, or by conventional synthetic procedures, in accordance with standard techniques, from available starting materials using appropriate reagents and reaction conditions. In this respect, the skilled person may refer to inter alia "Comprehensive Organic Synthesis" by B. M. Trost and I. Fleming, Pergamon Press, 1991. The substituents R¹, Q², Q³, Q^3a, Q⁴, R⁵, Z¹, Z², Z³, B, E, X, Y, L and A in final compounds of formula I or relevant intermediates may be modified one or more times, after or during the processes described above by way of methods that are well known to those skilled in the art. Examples of such methods include substitutions, reductions (e.g. of double bonds to single bonds by hydrogenation), oxidations, alkylations, acylations, hydrolyses, esterifications, etherifications and nitrations. The precursor groups can be changed to a different such group, or to the groups defined in formula I, at any time during the reaction sequence. In this respect, the skilled person may also refer to "Comprehensive Organic Functional Group Transformations" by A. R. Katritzky, O. Meth-Cohn and C. W. Rees, Pergamon Press, 1995.

For example, in the case where R¹ or R² represents a halo group, such groups may be inter- converted one or more times, after or during the processes described above for the preparation of compounds of formula I. Appropriate reagents include NiC^ (for the conversion to a chloro group). Further, oxidations that may be mentioned include oxidations of sulfanyl groups to sulfoxide and sulfonyl groups, for example employing standard reagents (e.g. mefø-chloroperbenzoic acid, KMnO₄ or a solution of Oxone® in ethylenediaminetetraacetic acid).

Other transformations that may be mentioned include the conversion of a halo group (preferably iodo or bromo) to a -CN or 1-alkynyl group (e.g. by reaction with a compound which is a source of cyano anions (e.g. sodium, potassium, copper (I) or zinc cyanide) or with a 1-alkyne, as appropriate). The latter reaction may be performed in the presence of a suitable coupling catalyst (e.g. a palladium and/or a copper based catalyst) and a suitable base (e.g. a tri-(Ci_6 alkyl)amine such as triethylamine, tributylamine or ethyldiisopropylamine). Further, amino groups and hydroxy groups may be introduced in accordance with standard conditions using reagents known to those skilled in the art.

Compounds of formula I may be isolated from their reaction mixtures using conventional techniques.

It will be appreciated by those skilled in the art that, in the processes described above and hereinafter, the functional groups of intermediate compounds may need to be protected by protecting groups. The protection and deprotection of functional groups may take place before or after a reaction in the above-mentioned schemes.

Protecting groups may be removed in accordance with techniques that are well known to those skilled in the art and as described hereinafter. For example, protected compounds/intermediates described herein may be converted chemically to unprotected compounds using standard deprotection techniques.

The type of chemistry involved will dictate the need, and type, of protecting groups as well as the sequence for accomplishing the synthesis.

The use of protecting groups is fully described in "Protective Groups in Organic Chemistry", edited by J W F McOmie, Plenum Press (1973), and "Protective Groups in Organic Synthesis", 3^rd edition, T.W. Greene & P. G. M. Wutz, Wiley-lnterscience (1999).

Medical and Pharmaceutical Uses

Compounds of the invention are indicated as pharmaceuticals. According to a further aspect of the invention there is provided a compound of the invention, as hereinbefore defined but without the provisos for use as a pharmaceutical.

Although compounds of the invention may possess pharmacological activity as such, certain pharmaceutically-acceptable (e.g. "protected") derivatives of compounds of the invention may exist or be prepared which may not possess such activity, but may be administered parenterally or orally and thereafter be metabolised in the body to form compounds of the invention. Such compounds (which may possess some pharmacological activity, provided that such activity is appreciably lower than that of the "active" compounds to which they are metabolised) may therefore be described as "prodrugs" of compounds of the invention. By "prodrug of a compound of the invention", we include compounds that form a compound of the invention, in an experimentally-detectable amount, within a predetermined time (e.g. about 1 hour), following oral or parenteral administration. All prodrugs of the compounds of the invention are included within the scope of the invention. Furthermore, certain compounds of the invention may possess no or minimal pharmacological activity as such, but may be administered parenterally or orally, and thereafter be metabolised in the body to form compounds (e.g. compounds of the invention) that possess pharmacological activity as such. Such compounds (which also includes compounds that may possess some pharmacological activity, but that activity is appreciably lower than that of the "active" compounds of the invention to which they are metabolised), may also be described as "prodrugs". Thus, the compounds of the invention are useful because they possess pharmacological activity, and/or are metabolised in the body following oral or parenteral administration to form compounds which possess pharmacological activity (e.g. similar or pronounced pharmacological activity as compared to the compounds of the invention from which they are formed). Compounds of the invention are particularly useful because they may inhibit the activity of a member of the MAPEG family.

Compounds of the invention are particularly useful because they may inhibit (for example selectively) the activity of prostaglandin E synthases (and particularly microsomal prostaglandin E synthase-1 (mPGES-1 )), i.e. they prevent the action of mPGES-1 or a complex of which the mPGES-1 enzyme forms a part, and/or may elicit a mPGES-1 modulating effect, for example as may be demonstrated in the test described below. Compounds of the invention may thus be useful in the treatment of those conditions in which inhibition of a PGES, and particularly mPGES-1 , is required.

Compounds of the invention are thus expected to be useful in the treatment of inflammation. Further, as the compounds of the invention may be of use as mPGES inhibitors (e.g. mPGES-1 inhibitors), they may also be useful in preventing or treating benign or malignant neoplasias (as they may reduce the production of PGE2). Hence, the compounds of the invention may also be useful in treating cancers. The term "inflammation" will be understood by those skilled in the art to include any condition characterised by a localised or a systemic protective response, which may be elicited by physical trauma, infection, chronic diseases, such as those mentioned hereinbefore, and/or chemical and/or physiological reactions to external stimuli (e.g. as part of an allergic response). Any such response, which may serve to destroy, dilute or sequester both the injurious agent and the injured tissue, may be manifest by, for example, heat, swelling, pain, redness, dilation of blood vessels and/or increased blood flow, invasion of the affected area by white blood cells, loss of function and/or any other symptoms known to be associated with inflammatory conditions.

The term "inflammation" will thus also be understood to include any inflammatory disease, disorder or condition per se, any condition that has an inflammatory component associated with it, and/or any condition characterised by inflammation as a symptom, including inter alia acute, chronic, ulcerative, specific, allergic and necrotic inflammation, and other forms of inflammation known to those skilled in the art. The term thus also includes, for the purposes of this invention, inflammatory pain, pain generally and/or fever. Where a condition has an inflammatory component associated with it, or a condition characterised by inflammation as a symptom, the skilled person will appreciate that compounds of the invention may be useful in the treatment of the inflammatory symptoms and/or the inflammation associated with the condition.

Accordingly, compounds of the invention may be useful in the treatment of asthma, chronic obstructive pulmonary disease, pulmonary fibrosis, inflammatory bowel disease, irritable bowel syndrome, inflammatory pain, fever, migraine, headache, low back pain, fibromyalgia, myofascial disorders, viral infections (e.g. influenza, common cold, herpes zoster, hepatitis C and AIDS), bacterial infections, fungal infections, dysmenorrhea, burns, surgical or dental procedures, malignancies (e.g. breast cancer, colon cancer, and prostate cancer), hyperprostaglandin E syndrome, classic Bartter syndrome, atherosclerosis, gout, arthritis, osteoarthritis, juvenile arthritis, rheumatoid arthritis, rheumatic fever, ankylosing spondylitis, Hodgkin's disease, systemic lupus erythematosus, vasculitis, pancreatitis, nephritis, bursitis, conjunctivitis, iritis, scleritis, uveitis, wound healing, dermatitis, eczema, psoriasis, stroke, diabetes mellitus, neurodegenerative disorders such as Alzheimer's disease and multiple sclerosis, autoimmune diseases, allergic disorders, rhinitis, ulcers, coronary heart disease, sarcoidosis and any other disease with an inflammatory component. Compounds of the invention may also have effects that are not linked to inflammatory mechanisms, such as in the reduction of bone loss in a subject. Conditions that may be mentioned in this regard include osteoporosis, osteoarthritis, Paget's disease and/or periodontal diseases. Compounds the invention may thus also be useful in increasing bone mineral density, as well as the reduction in incidence and/or healing of fractures, in subjects. Compounds of the invention are indicated both in the therapeutic and/or prophylactic treatment of the above-mentioned conditions. According to a further aspect of the present invention, there is provided a method of treatment of a disease which is associated with, and/or which can be modulated by inhibition of, a member of the MAPEG family such as a PGES (e.g. mPGES-1 ), LTC₄ synthase and/or FLAP and/or a method of treatment of a disease in which inhibition of the activity of a member of the MAPEG family such as PGES (and particularly mPGES-1 ), LTC₄ synthase and/or FLAP is desired and/or required (e.g. inflammation), which method comprises administration of a therapeutically effective amount of a compound of the invention, as hereinbefore defined but without the provisos, to a patient suffering from, or susceptible to, such a condition.

"Patients" include mammalian (including human) patients. The term "effective amount" refers to an amount of a compound, which confers a therapeutic effect on the treated patient. The effect may be objective (i.e. measurable by some test or marker) or subjective (i.e. the subject gives an indication of or feels an effect). Compounds of the invention will normally be administered orally, intravenously, subcutaneously, buccally, rectally, dermally, nasally, tracheally, bronchially, sublingually, by any other parenteral route or via inhalation, in a pharmaceutically acceptable dosage form. Compounds of the invention may be administered alone, but are preferably administered by way of known pharmaceutical formulations, including tablets, capsules or elixirs for oral administration, suppositories for rectal administration, sterile solutions or suspensions for parenteral or intramuscular administration, and the like. Such formulations may be prepared in accordance with standard and/or accepted pharmaceutical practice.

According to a further aspect of the invention there is thus provided a pharmaceutical formulation including a compound of the invention, as hereinbefore defined but without the provisos, in admixture with a pharmaceutically acceptable adjuvant, diluent or carrier.

Depending on e.g. potency and physical characteristics of the compound of the invention (i.e. active ingredient), pharmaceutical formulations that may be mentioned include those in which the active ingredient is present in at least 1 % (or at least 10%, at least 30% or at least 50%) by weight. That is, the ratio of active ingredient to the other components (i.e. the addition of adjuvant, diluent and carrier) of the pharmaceutical composition is at least 1 :99 (or at least

10:90, at least 30:70 or at least 50:50) by weight.

The invention further provides a process for the preparation of a pharmaceutical formulation, as hereinbefore defined, which process comprises bringing into association a compound of the invention, as hereinbefore defined but without the provisos, or a pharmaceutically acceptable salt thereof with a pharmaceutically-acceptable adjuvant, diluent or carrier.

Compounds of the invention may also be combined with other therapeutic agents that are useful in the treatment of inflammation (e.g. NSAIDs and coxibs).

According to a further aspect of the invention, there is provided a combination product comprising:

(A) a compound of the invention, as hereinbefore defined but without the provisos; and

(B) another therapeutic agent that is useful in the treatment of inflammation, wherein each of components (A) and (B) is formulated in admixture with a pharmaceutically- acceptable adjuvant, diluent or carrier.

Such combination products provide for the administration of a compound of the invention in conjunction with the other therapeutic agent, and may thus be presented either as separate formulations, wherein at least one of those formulations comprises a compound of the invention, and at least one comprises the other therapeutic agent, or may be presented (i.e. formulated) as a combined preparation (i.e. presented as a single formulation including a compound of the invention and the other therapeutic agent). Thus, there is further provided:

(1 ) a pharmaceutical formulation including a compound of the invention, as hereinbefore defined but without the provisos, another therapeutic agent that is useful in the treatment of inflammation, and a pharmaceutically-acceptable adjuvant, diluent or carrier; and

(2) a kit of parts comprising components:

(a) a pharmaceutical formulation including a compound of the invention, as hereinbefore defined but without the provisos, in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier; and

(b) a pharmaceutical formulation including another therapeutic agent that is useful in the treatment of inflammation in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier, which components (a) and (b) are each provided in a form that is suitable for administration in conjunction with the other.

The invention further provides a process for the preparation of a combination product as hereinbefore defined, which process comprises bringing into association a compound of the invention as hereinbefore defined but without the provisos with another therapeutic agent that is useful in the treatment of inflammation, and a pharmaceutically-acceptable adjuvant, diluent or carrier.

By "bringing into association", we mean that the two components are rendered suitable for administration in conjunction with each other.

Thus, in relation to the process for the preparation of a kit of parts as hereinbefore defined, by bringing the two components "into association with" each other, we include that the two components of the kit of parts may be:

(i) provided as separate formulations (i.e. independently of one another), which are subsequently brought together for use in conjunction with each other in combination therapy; or

(ii) packaged and presented together as separate components of a "combination pack" for use in conjunction with each other in combination therapy. Compounds of the invention may be administered at varying doses. Oral, pulmonary and topical dosages may range from between about 0.01 mg/kg of body weight per day (mg/kg/day) to about 100 mg/kg/day, preferably about 0.01 to about 100 mg/kg/day, and more preferably about 0.1 to about 25 mg/kg/day. For e.g. oral administration, the compositions typically contain between about 0.01 mg to about 5000 mg, and preferably between about 1 mg to about 2000 mg, of the active ingredient. Intravenously, the most preferred doses will range from about 0.001 to about 10 mg/kg/hour during constant rate infusion. Advantageously, compounds may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily.

In any event, the physician, or the skilled person, will be able to determine the actual dosage which will be most suitable for an individual patient, which is likely to vary with the route of administration, the type and severity of the condition that is to be treated, as well as the species, age, weight, sex, renal function, hepatic function and response of the particular patient to be treated. The above-mentioned dosages are exemplary of the average case; there can, of course, be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention.

Compounds of the invention may have the advantage that they are effective, and preferably selective, inhibitors of a member of MAPEG family, e.g. inhibitors of prostaglandin E synthases (PGES) and particularly microsomal prostaglandin E synthase-1 (mPGES-1 ). The compounds of the invention may reduce the formation of the specific arachidonic acid metabolite PGE₂ without reducing the formation of other COX generated arachidonic acid metabolites, and thus may not give rise to the associated side-effects mentioned hereinbefore. Compounds of the invention may also have the advantage that they may be more efficacious than, be less toxic than, be longer acting than, be more potent than, produce fewer side effects than, be more easily absorbed than, and/or have a better pharmacokinetic profile (e.g. higher oral bioavailability and/or lower clearance) than, and/or have other useful pharmacological, physical, or chemical properties over, compounds known in the prior art, whether for use in the above-stated indications or otherwise. Biological Test

Microsomes from Rosetta E.coli bacteria expressing recombinant human mPGES-1 is dissolved in 0.1 M KPj pH 7.4 buffer containing 2,5 mM GSH. 50 μl of the enzyme is subsequently dispensed in a 384-well plate. 0,5 μl of the inhibitor dissolved in DMSO at is thereafter added to each well and incubated for 25 minutes at room temperature.

Subsequently, 2 μl of PGH2 dissolved in an appropriate solvent is added to each well and after one minute the acidified stop solution containing FeCI₂ is added. 4 μl of the total volume is transferred to a separate plate and diluted 750-fold in two separate steps before HTRF detection of PGE2. The HTRf detection is performed by the use of a commercially available kit from CisBio essentially according to the manufacturer's protocol. Briefly, 10 μl of the diluted sample is transferred to a white 384-well plate. 5 μl of d2 and 5μl Eu3+-Cryptate labeled anti-PGE₂ is added to each well containing samples by the use of a Multidrop. The plate is covered with a plastic self-adhesive film, centrifuged at 1200 rpm for 1 minute and subsequently stored at +4⁰C over night.

After over night incubation the fluorescence is measured by the use of an appropriate microplate reader. The fluorescence of europium cryptate, and d2 are measured using the following excitation and emission wavelength, europium cryptate: A_max ^ex = 307 nm, λ_max ^em = 620 nm and d2: Λ_max ^ex = 620 nm, A_max ^em = 665 nm), respectively. The extent of the specific FRET is measured as a ratio of the emission intensity at 665 nm vs. that at 620 nm. A standard curve using synthetic PGE2 is used to quantify the amount of PGE2 in unknown samples.

Chemical Examples

Unless otherwise stated, one or more tautomeric forms of compounds of the examples described hereinafter may be prepared in situ and/or isolated. All tautomeric forms of compounds of the examples described hereinafter should be considered to be disclosed.

The invention is illustrated by way of the following examples, in which the following abbreviations may be employed:

AIBN Azo-bis-isobutyronitrile aq. aquaeous solution Boc te/f.-butoxycarbonyl

DIC diisopropyl-carbodiimide

DIPEA Λ/-ethyl-diisopropylamine

DMSO dimethylsulphoxide

DMF Λ/,Λ/-dimethylformamide sat. saturated h hour(s)

HATU O-(7-azabenzotriazol-1-yl)-Λ/,Λ/,Λ/',Λ/-tetramethyluronium-hexafluorophosphate

HBTU O-Benzotriazole-1-yl-Λ/,Λ/,Λ/',Λ/-tetramethyluronium-hexafluorophosphate

DPPA Diphenylphosphoryl azide

HPLC high performance liquid chromatography i. vac. in vacuo cone. concentrated min minute(s)

MS mass spectrometry

NBS Λ/-bromo-succinimide

NMM Λ/-methyl-morpholine

NMP Λ/-methyl-pyrrolidin-2-one o ortho

PfTU O-pentafluorophenyl-Λ/,Λ/,Λ/',Λ/-tetramethyluronium-hexafluorophosphate

PPA propanephosphonic acid cycloanhydride quant. quantitative

Rf retention factor

Rt retention time mp melting point rac. Racemic

M mol / L sat. saturated

TBME tert. -butyl-methyl-ether

TBTU O-(benzotriazol-1-yl)-Λ/,Λ/,Λ/',Λ/'-tetramethyluronium tetrafluoroborate

TEA triethylamine

TFA trifluoroacetic acid

THF tetrahydrofuran tert. tertiary

TLC Thin layer chromatography ∑ yield over all the steps carried out analogously as described

KHCO₃ potassium-hydrogen-carbonate

K₂CO₃ potassium carbonate

Na₂SO₄ sodium sulfate

NaOH sodium hydroxide

HCI hydrochloric acid

DCC Λ/jΛ/'-Dicyclohexylcarbodiimide

DIBAL-H Diisobutylaluminium hydride

DMAP 4-Dimethylaminopyridine

EDC 3-(3-Dimethylaminopropyl)-1 -ethyl-carbodiimide

EDCI 3-(3-Dimethylaminopropyl)-1 -ethyl-carbodiimide hydrochloride

The HPLC/MS data, where specified, were obtained under the following conditions:

Agilent 1 100 with quarternary pump, Gilson G215 Autosampler, HP diode array detector.

The following was used as the mobile phase:

E1 : water with 0.15% formic acid

E2: acetonitrile

E3: water with 0.1% acetic acid

Eluent gradient A (polar): time in min %E1 %E2 flov

0.0 95 5 1.6

4.00 50 50 1.6

4.50 10 90 1.6

5.00 10 90 1.6

5.50 90 10 1.6

Eluent gradient B (standard): time in min %E1 %E2 flow rate in mL/min

0.0 95 5 1.6

4.50 10 90 1.6

5.00 10 90 1.6

5.50 90 10 1.6 Eluent gradient C (unpolar): time in min %E1 %E2 flow rate in mL/min

0.0 95 5 1 .6

2.00 10 90 1 .6

5.00 10 90 1 .6

5.50 90 10 1 .6

Eluent gradient D (ultrakurz-polar): time in min %E1 %E2 flov

0.0 95 5 1.6

2.00 50 50 1.6

2.25 10 90 1.6

2.5 10 90 1.6

2.75 95 5 1.6

Eluent gradient E (ultrakurz-standard): time in min %E1 %E2 flow rate in mL/min

0.0 95 5 1.6

2.25 10 90 1.6

2.5 10 90 1.6

2.75 95 5 1.6

Eluent gradient F (ultakurz-unpolar): time in min %E1 %E2 flow rate in mL/min 0.0 95 5 1.6

1.00 10 90 1.6

2.5 10 90 1.6

2.75 95 5 1.6 Eluent gradient G : time in min %E3 %E2 flow rate in mL/min

0.0 95 5 3

0.3 95 5 3

2 2 98 3

2.4 2 98 3

2.45 95 5 3

2.8 95 5 3

The following was used as the stationary phase: (column temperature: constant at 25°C)

1 : Zorbax StableBond C18, 3.5μm, 4.6x75mm 2: Waters Symmetry C18, 3.5μm, 4.6x75mm

3: Zorbax Bonus-RP C18, 3.5μm, 4.6x75mm

4: YMC-Pack ODS-AQ, 3μm, 4.6x75mm

5: XBridge C18, 3.5μm, 4.6x75mm

7:Zobrax Stable Bond C18, 1.8μm, 3,0x30mm 8: Sunfire C18, 2.5μm, 3.0x30mm

9: Xbridge C1 , 2,5μm, 3,0x30mm

12:Zorbax Stable Bond C18, 3.5μm, 4.6x75mm

The following was used as the stationary phase: (column temperature: constant at 20⁰C)

10:lnterchim Strategy C18, 5μm, 4,6x50mm 1 1 :XRS C18, 5μm, 4,6x50mm

The method is abbreviated using the above descriptions (eg. A1 for Eluent gradient A with stationary phase 1 ).

The diode array detection took place in a wavelength range from 210-550 nm Range of mass-spectrometric detection: m/z 120 to m/z 1000 Alternatively, the following method was used, abbreviated CC:

HP1100 HPLC + DAD (Wavelength range: 210nm to 500nm), and Gilson 215 Autosampler

RP-HPLC MS analyses were performed on a Waters ZQ2000 mass spectrometer

The following was used as the mobile phase: E1 : water with 0.1% trifluoracetic acid E2: acetonitrile with 0.1 % trifluoracetic acid

Eluent gradient: time in min %E1 %E2 flow rate in mL/min

0.0 95 5 1.5

2.00 0 100 1.5

2.50 0 100 1.5

2.60 95 5 1.5

The following was used as the stationary phase:

Sunfire C18 4.6x50mm, 3.5μm (column temperature: constant at 40⁰C)

The diode array detection took place in a wavelength range from 210-500 nm Range of mass-spectrometric detection: m/z 120 to m/z 820.

Alternatively, the following method was used, abbreviated EX1 :

Column: Atlantis dC18 5 mm, 2.1x50 mm. Mobile phase: 10-95% MeCN in 0.01 % TFA. Flow rate: 0.2 mL/min. Detection: UV 254 nm.

Alternatively, the following method was used, abbreviated EX2:

Column: Acquity UPLC BEH SHIELD RP18 1.7 mm, 2.1x100 mm. Mobile phase: 5-100% MeCN in 0.1 % HCOOH. Flow rate: 0.2 mL/min. Detection: UV 254 nm / 21 1 nm. The following compounds are accompanied by structural drawings. The skilled person will appreciate that the rules of valency must be adhered to and hence there must be a certain number of bonds attached to each atom, which may not necessarily be depicted on the drawings. For example, in the case where a nitrogen heteroatom is depicted with only one or two bonds attached to it, the skilled person will realise that it should be attached to an addional one or two bonds (a total of three), in which such bonds are normally attached to one or two hydrogen atoms (so forming a -NH₂ or -N(H)- moiety).

Example 83

2-(2,6-Dichloro-phenylamino)-3H-imidazo[4,5-c1pyridine-6-carboxylic acid (4,4-dimethyl- cvclohexyD-amide

(83a) 4-Amino-pyridine-2-carboxylic acid (4,4-dimethyl-cvclohexyl)-amide To a mixture of 4-amino-pyridine-6-carboxylic acid (250 mg, 1 ,81 mmol) with TBTU (600mg, 1 ,87 mmol) and TEA (1 ml_, 7.2 mmol) in 4 ml. DMF was added 4,4-dimethyl- cyclohexylamine (300 mg, 1.83 mmol) and the mixture stirred at ambient temperature for 1 h. Then the mixture was poured into water at 0⁰C and stirred for 5 min. The precipitate was filtered off, washed with water and dried at 55°C. Yield: 340 mg (76%) Rt value: 2.38 min (B2) Mass spectrum: (M+H)⁺ = 248

(83b) 4-Nitramino-pyridine-2-carboxylic acid (4,4-dimethyl-cvclohexyl)-amide Fuming nitric acid (2.0 ml.) was added to a mixture of the product obtained in 83a (340 mg, 1.38 mmol) and cone, sulphuric acid (4ml_) under cooling at 0⁰C and stirred for 30 min at 0⁰C. The mixture was poured into water at 0⁰C and adjusted to pH 2 by adding cone. ammonia. The mixture was filtrated, and the solid washed with water and dried overnight at ambient temperature. Yield: 360 mg (90%); R₁ value: 4.20 min (B2) ; Mass spectrum: (M+H)⁺ = 293 (83c) 4-Amino-5-nitro-pyridine-2-carboxylic acid (4,4-dimethyl-cvclohexyl)-amide The product obtained in 83b (360 mg, 1.23 mmol) was stirred at 85°C in cone, sulphuric acid (5 ml.) for 5 min. The mixture was poured into water at 0⁰C and adjusted to pH 3-4 by adding cone, ammonia. The mixture was extracted with dichloromethane, the organic layers dried over MgSO₄ and concentrated i. vac. The residue was purified via HPLC (C-18 Symmetry, eluent-gradient: (water + 0.15% HCOOH) / acetonitrile = 90:10 -> 0:100). Yield: 100 mg (28%) Rt value: 4.46 min (B2)

Mass spectrum: (M+H)⁺ = 293

(83d) 4,5-Diamino-pyridine-2-carboxylic acid (4,4-dimethyl-cvclohexyl)-amide The product obtained in 83c (100 mg, 0.34 mmol) in 10 ml. methanol was mixed with Raney nickel (25 mg) and hydrogenated at 3.4 bar for 2.5 h in a Parr apparatus. The mixture was filtered and the filtrate concentrated /^'. vac. Yield: 80 mg (89%) R_t value: 2.47 min (B2) Mass spectrum: (M+H)⁺ = 263

(83e) 2-(2,6-Dichloro-phenylamino)-3H-imidazo[4,5-c1pyridine-6-carboxylic acid (4,4- dimethyl-cvclohexyD-amide

To the product obtained in 83d (75 mg, 0.29 mmol) in 4 ml. DMF was added 1 ,3-dichloro-2- isocyanato-benzene and the mixture stirred at ambient temperature for 16 h. Then DIC (55 μl_, 0.35 mmol) was added and the mixture stirred at ambient temperature for 20 h. 1 ml_ methanol was added and the product purified via RP-HPLC (C-18 symmetry; eluent-gradient: water+HCOOH / acetonitrile = 90:10 -> 0:100).

Yield: 28%

Rt value: 3.58 min (C2) Mass spectrum: (M+H)⁺ = 432/434/436 (chlorine isotopes)

In analogy with the above described example, the following compounds were prepared:

Example 360

2-(2-Chloro-6-fluoro-phenylamino)-3-methyl-N-(4-trifluoromethyl-pyridin-2-yl)-3-H- imidazo[4,5-c1pyridine-6-carboxamide

(360a) 2-Bromo-5-fluoro-pyridine 1 -oxide

2-Bromo-5-fluoropyridine (15 g, 85.6 mmol), trifluoroacetic acid (70 ml_, 908 mmol) and hydrogen peroxide (35% in water) (9 ml_, 105 mmol) were stirred overnight at 70⁰C. The mixture was poured into water and extracted with dichloromethane. The organic layers were washed with NaHCO₃ (aq), dried over MgSO₄ and the solvent was removed i.vac. Yield: 15.2 g (93%)

(360b) 2-Bromo-5-fluoro-4-nitropyridine 1 -oxide

Prepared analogously to example 83b from 2-bromo-5-fluoropyridine 1 -oxide, cone, sulphuric acid and cone, nitric acid Yield: 1 1 g (60%) (360c) 2-Bromo-5-(methylamino)-4-nitropyridine 1 -oxide

2-Bromo-5-fluoro-4-nitropyridine 1 -oxide (11.2 g, 47 mmol) and 2 N methylamine (solution in THF) (48 ml_, 96 mmol) in 150 ml. THF was stirred for 5 min at ambient temperature. Water was poured into the mixture and THF was removed i.vac. The solid was filtered, washed with water and dried at 50⁰C i. vac. Yield: 10 g (86%)

(36Od) 6-Bromo-N-methyl-4-nitropyridin-3-amine 2-Bromo-5-(methylamino)-4-nitropyridine 1 -oxide (10.0 g, 40.4 mmol) in 20O mL dichloromethane was mixed with phosphorous tribromide (10 ml_, 106 mmol) in 50 ml_ dichloromethane at 10⁰C and stirred for 10 minutes at ambient temperature. Water and methanol were added and the mixture was extracted with dichloromethane. The organic layers were washed with Na₂CC>3 (aq.) dried over MgSO₄ The residue was triturated with ethanol, filtered and dried at 50⁰C i. vac. Yield: 7.7 g (83%)

(36Oe) 6-Bromo-N³-methylpyridine-3,4-diamine

Prepared analogously to example 83d by hydrogenation of 6-bromo-N-methyl-4-nitropyridin- 3-amine using Raney nickel in THF. Yield: quant, mass spectrum: (M+H)⁺ = 202/204 (bromine isotopes)

(36Of) 1-(4-Amino-6-bromopyridin-3-yl)-3-(2-chloro-6-fluorophenyl)-1-methyl-thiourea To the product obtained in (36Oe) (2.61 g, 12.9 mmol) in 20 ml. THF and 10 ml. methanol was added i-chloro-S-fluoro^-isothiocyanatobenzene (2.42 g, 12.9 mmol) and the mixture stirred at ambient temperature for 16h. 25 ml. methanol and 75 ml. water were added and the mixture supersounded for 10 min. The precipitate was filtered off, washed with a mixture water/methanol = 2:1 and dried at 60°C. Yield: 4.23 g (84%) mass spectrum: (M+H)⁺ = 389/391/393 (chlorine and bromine isotopes)

R_t value: 1.72 min (E7) (36Og) 6-Bromo-N-(2-chloro-6-fluorophenyl)-3-methyl-3H-imidazo[4,5-c1pyridin-2 -amine To the product obtained in (36Of) (4.23 g, 10.9 mmol) in 25 ml. DMF was added DIC (3.5 mL, 22.4 mmol) and the mixture stirred at 100⁰C for 3d. Methanol and cone, ammonia were added and the product purified by RP-HPLC (C-18 X-Bridge; eluent-gradient: water+0.15% cone, ammonia / acetonitrile = 90:10 -> 0:100). The product fractions were concentrated /^'. vac, the precipitate was filtered off, washed with water and dried at 60⁰C. Yield: 1.36 g (35%) mass spectrum: (M+H)⁺ = 355/357/359 (chlorine and bromine isotopes) Rt value: 1.59 min (E7)

(36Oh) Methyl 2-(2-chloro-6-fluorophenylamino)-3-methyl-3H-imidazo[4,5-c1pyridine-6- carboxylate

To 6-bromo-N-(2-chloro-6-fluorophenyl)-3-methyl-3H-imidazo[4,5-c]pyridin-2-amine (1.36 g, 3.8 mmol) in 25 mL methanol and 5 mL DMF was added 1 ,V-Bis-(diphenylphosphino)- ferrocene (150 mg, 0.27 mmol) and palladium-(ll)-acetate (60 mg, 0.27 mmol) at 80⁰C and stirred for 14 h under carbon monoxide at 6.5 bar. The mixture was filtered and the solvent removed i. vac. The residue was purified by HPLC (C18 StableBond, 8μm, eluent: H₂O +

0.15% HCOOH + 10-100% acetonitrile). Yield: 1 g (80%) mass spectrum: (M+H)+ = 335/337/339 (chlorine isotopes)

Rt value: 1.23 min (E9)

(36Oi) 2-(2-Chloro-6-fluorophenylamino)-3-methyl-N-(4-trifluoromethyl-pyridin-2-yl)-3-H- imidazo[4,5-c1pyridine-6-carboxamide

To 2-amino-4-trifluoromethyl-pyridine (60 mg, 0.37 mmol) in 3 mL THF was added trimethylaluminium (1 N in hexane, 400μL, 0.40 mmol) and stirred at ambient temperature for 10 min. Then methyl 2-(2-chloro-6-fluorophenylamino)-3-methyl-3H-imidazo[4,5-c]pyridine-6- carboxylate (100 mg, 0.30 mmol) was added and stirred at 40°C for 16h. Methanol and cone. acetic acid were added and the product purified by RP-HPLC (C-18 StableBond; eluent- gradient: water+0.15% HCOOH / acetonitrile = 90:10 -> 0:100). The product fractions were concentrated /^'. vac, made alkaline with ammonia, the precipitate was filtered off, washed with water and dried at 60⁰C. Yield: 67 mg (48%) mass spectrum: (M+H)⁺ = 465/467/469 (chlorine isotopes); R₁ value: 2.17 min (E7) In analogy with the above described example, the following compounds were prepared:

Example 366

2-(3,5-Dichloropyridin-4-ylamino)-N-(4-trifluoromethyl-pyridin-2-yl)-1 H-imidazo[4,5-c1pyridine- 6-carboxamide

(366a) Methyl-4-aminopicolinate

A mixture of 4-aminopicolinic acid (2.50 g, 18.1 mmol) and cone, sulfuric acid (2 ml_, 37.3 mmol) in 50 ml. methanol was refluxed for 20 h. The mixture was concentrated /^'. vac, then

50 ml. dichloromethane were added and made basic by addition of cone, ammonia (aq).

Then water and NaCI (aq, sat.) were added and the aqueous layer was extracted with methylenechloride / methanol = 9:1. The combined organic layers were dried over MgSO₄ and then evaporated to dryness. Yield: 2.05 g (74%) mass spectrum: (M+H)⁺ = 153

(366b) Methyl-4-amino-5-nitropicolinate

Prepared analogously to example 83b from methyl-4-aminopicolinate, cone, sulphuric acid and potassium nitrate.

Yield: 55%; mass spectrum: (M+H)⁺ = 198; R₁ value: 1.05 min (E7) (366c) 4-Amino-5-nitropicolinic acid

A mixture of methyl-4-amino-5-nitropicolinate (200 mg, 1.01 mmol) and NaOH (aq, 1 M, 2.0 ml_, 2.0 mmol) in methanol was stirred at ambient temperature for 10 min. The mixture was acidified by addition of HCI (aq, 1 M, 2.1 ml_, 2.1 mmol) and concentrated /^'. vac. and the aqueous residing mixture filtered. The solid was washed with water and dried /^'. vac. Yield: 180 mg (97%)

(366d) 4-Amino-5-nitro-N-(4-trifluoromethyl-pyridin-2-yl)-picolinamide A mixture of 4-amino-5-nitropicolinic acid (180 mg, 1.0 mmol) in 10 ml. dichloromethane with 280 μl_ thionyl chloride was refluxed for 1 h, cooled to ambient temperature and concentrated to dryness /^'. vac. The residue was mixed with 10 ml. THF and 10 ml. dichloromethane and pyridine (100 μl_, 1.3 mmol), and 4-trifluoromethyl-pyridin-2 -amine (160 mg, 1.0 mmol) was added to the mixture. After stirring for 45 min the mixture was disturbed with methanol and concentrated /.vac. The residue was was purified by chromatography on HPLC (C18 StableBond, eluent: H₂O + 0.15% HCOOH + 10-100% acetonitrile) Yield: 160 mg (50%) mass spectrum: (M+H)+ = 328 R_t value: 2.15 min (E7)

(366e) 4,5-Diamino-N-(4-trifluoromethyl-pyridin-2-yl)-picolinamide Prepared analogously to example 83d by hydrogenation of 4-amino-5-nitro-N-(4- trifluoromethyl-pyridin-2-yl)-picolinamide using palladium/charcoal 10% in methanol and THF. Yield: 96% mass spectrum: (M+H)+ = 298 R_t value: 1.42 min (E7)

(366f) 5-Amino-4-(3-(3,5-dichloropyridin-4-yl)-N-(4-trifluoromethyl-pyridin-2-yl)-picolinamide Prepared analogously to example 36Of from 4,5-diamino-N-(4-trifluoromethyl-pyridin-2-yl)- picolinamide and 3,5-dichloro-4-isothiocyanatopyridine in THF and acetonitrile. Yield: 76% mass spectrum: (M+H)⁺ = 502 R_t value: 1.97 min (E7) (366e) 2-(3,5-Dichloropyridin-4-ylamino)-N-(4-trifluoromethyl-pyridin-2-yl)-1 H-imidazo[4,5- cipyridine-6-carboxamide

Prepared analogously to example 36Og from 5-amino-4-(3-(3,5-dichloropyridin-4-yl)-N-(4- trifluoromethyl-pyridin-2-yl)-picolinamide and DIC in acetonitrile

Yield: 89% mass spectrum: (M+H)+ = 468/470/472 (chlorine isotopes)

R_t value: 2.02 min (E7)

Example 396

N-(4-tert.-Butyl-pyridin-2-yl)-2-(2,6-dichlorophenylamino)-1 H-imidazo[4,5-c1pyridine-6- carboxamide

(396a) Methyl-4-aminopicolinate

Prepared analogously to example 366a from 4-aminopicolinic acid and sulfuric acid in methanol. Yield: 74% mass spectrum: (M+H)⁺ = 153

(396b) Methyl-4-amino-5-nitro-picolinate Prepared analogously to example 83b from methyl-4-aminopicolinate, cone, sulphuric acid and potassium nitrate. Yield: 55% mass spectrum: (M+H)⁺ = 198 Rt value: 1.05 min (E7)

(396c) Methyl 4,5-diamino-picolinate

Prepared analogously to example 83d by hydrogenation of methyl-4-amino-5-nitropicolinate using palladium/charcoal 10% in THF.

Yield: 98%

(396d) Methyl 5-amino-4-(3-(2,6-dichlorophenyl)thioureido)picolinate

Prepared analogously to example 36Of from methyl 4,5-diamino-picolinate and 1 ,3-dichloro-

2-isothiocyanato-benzene in THF and methanol.

Yield: 90% (396e) Methyl 2-(2,6-dichlorophenylamino)-1 H-imidazo[4,5-c1pyridine-6-carboxylate Prepared analogously to example 36Og from methyl 5-amino-4-(3-(2,6-dichlorophenyl)- thioureido)-picolinate and DIC in acetonitrile Yield: 94% mass spectrum: (M+H)+ = 337/39/41 (chlorine isotopes)

(396f) N-(4-tert.-Butylpyridin-2-yl)-2-(2,6-dichlorophenylamino)-1 H-imidazor4,5-clpyridine-6- carboxamide Prepared analogously to example 36Oi from methyl 2-(2,6-dichlorophenylamino)-1 H- imidazo[4,5-c]pyridine-6-carboxylate, 2 M trimethyl aluminium (solution in hexane)_and 4- tert.-butylpyridin-2-amine in THF.

Yield: 37% mass spectrum: (M+H)⁺ = 455/57/59 (chlorine isotopes) Rt value: 1.8 min (E9)

Biological Data

Title compounds of the examples were tested in the biological test described above and were found to exhibit 50% inhibition of mPGES-1 at a concentration of 10 μM or below. For example, the following representative compounds of the examples exhibited the following percentage inhibitions at 10 μM (unless otherwise specified):

Claims

1. Compounds of formula I,

in which

Q², Q³, Q^3a and Q⁴ respectively represent

R¹ represents halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4,

-N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14, -C(O)R^y15;

Ci-7 alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl [which latter four groups are optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and -C(O)R^y15]; or heterocycloalkyl or heteroaryl (which latter two groups are optionally substituted by one or more substituents selected from R⁹);

R², R³, R^3a and R⁴: independently represent hydrogen, halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4,

Ci-7 alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl [which latter four groups are optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11,

-S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and -C(O)R^y15] provided that if R³ or R^3a is a substituted Ci alkyl group, then the substituent cannot be -N(R^y5)-S(O)₂-R^y6; or any adjacent pair of R¹, R², R³, R^3a and R⁴ (i.e. R¹ and R^3a, R² and R³, R³ and R⁴ and R⁴ and R^3a) may be linked together to form, along with the essential carbon atoms of the Q² to Q⁴-containing ring to which they are necessarily attached, a further 5- to 7-membered ring, optionally containing one to three heteroatoms, which ring may contain one or two further unsaturations and which is optionally substituted by one or more Ci_-3 alkyl and/or =0 substituents;

R⁶ represents hydrogen; heterocycloalkyl, aryl, heteroaryl (which latter three groups are optionally substituted by one or more substituents selected from R⁹); or Ci-₆ alkyl, C_2-6 alkenyl, C_2-6 alkynyl, cycloalkyl, which latter four groups are optionally substituted by one or more substituents selected from fluoro, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂N(R^y13)R^y14, -C(O)R^y15, heterocycloalkyl, cycloalkyl, aryl and heteroaryl (which latter four groups are optionally substituted by one or more substituents selected from R⁹);

each R⁸ independently represents hydrogen, halo, -N(R^y1)R^y2, -OR^y1°, -S(O)₂-R^y11; Ci-6 alkyl, C_2-6 alkenyl, C_2-6 alkynyl, cycloalkyl, -0-Ci_-6 alkyl, -0-C_2-6 alkenyl,

-0-C_2-6 alkynyl, -O-cycloalkyl, -O-heterocycloalkyl [which latter nine groups are optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14, -C(O)R^y15, cycloalkyl, heterocycloalkyl, aryl and heteroaryl (which latter four groups are optionally substituted by one or more substituents selected from R⁹)]; heterocycloalkyl or heteroaryl (which latter two groups are optionally substituted by one or more substituents selected from R⁹);

A represents aryl, heteroaryl, heterocycloalkyl, cycloalkyl, C_M2 alkyl,

C_2-I2 alkenyl or C_2--_I2 alkynyl, all of which are optionally substituted by one or more substituents selected from R⁹; R⁹ represents, on each occasion when used herein: halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and -C(O)R^y15;

Ci-₇ alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl, heterocycloalkyl [which latter five groups are optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and -C(O)R^y15]; or aryl or heteroaryl [which latter two groups are optionally substituted by one or more substituents selected from halo, -CN, Ci_-7 alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl (which latter four groups are optionally substituted by one or more substituents selected from fluoro and -OR^*2), -0-Ci_-7 alkyl, -0-C_2-7 alkenyl, -0-C_2-7 alkynyl and -O-cycloalkyl (which latter four groups are optionally substituted by one or more fluoro atoms)]; or any two R⁹ substituents: when attached to the adjacent atoms of the A group; and, in the case where the R⁹ substituents are attached to a non-aromatic A group, when attached to the same atoms, may be linked together to form, together with the essential atoms of the A group to which the relevant R⁹ substituents are necessarily attached, a further 3- to 8-membered ring, optionally containing a further one or two heteroatoms, and which further ring optionally contains one or two unsaturations and which is optionally substituted by one or more Ci_-3 alkyl and/or =0 substituents;

m represents 0, 1 or 2;

each R^y4, R*⁶, R^y11 and R^y15: independently represent Ci_-7 alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl, which latter four groups are optionally substituted by one or more fluoro atoms; each R*², R^y1, R^y2, R*\ R^y5, R^y7, R^y8, R^y9, R^y1°, R^y12, R^y13 and R^y14: independently represent hydrogen or Ci_-7 alkyl, C_2-7 alkenyl, C_2-7 alkynyl, cycloalkyl, heterocycloalkyl, which latter five groups are optionally substituted one or more substituents selected from fluoro and -OC-ι-₃ alkyl; or any two groups, when attached to the same nitrogen atom (i.e. R^y1 and R*², R^y8 and R*⁹, and R^y13 and R^y14), may, together with that nitrogen atom to which they are necessarily attached, be linked together to form a 3- to 8-membered ring, optionally containing one or two further heteroatoms and which ring optionally contains one or two unsaturations and is optionally substituted by one or more

Ci-₃ alkyl and/or =0 substituents,

or a pharmaceutically acceptable salt thereof.

2. Compounds according to claim 1 , wherein

Q² represents -C(R²)=; and any two of Q³, Q^3a and Q⁴ respectively represent -C(R³)=, -C(R^3a)= and -C(R⁴)=; and the remaining one of Q³, Q^3a and Q⁴ represents -N=.

3. Compounds according to any one of the preceding claims, wherein

Q², Q³ and Q^3a respectively represent -C(R²)=, -C(R³)= and -C(R^3a)=; and

Q⁴ represents -N=.

4. Compounds according to any one of the claims 1 or 2, wherein

Q³ represents -N=; or wherein

Q², Q³ and Q⁴ respectively represent -C(R²)=, -C(R³)= and -C(R⁴)=; and Q^3a represents -N=.

5. Compounds according to any one of the preceding claims, wherein R¹ represents Ci_-4 alkyl (optionally substituted by one or more fluoro atoms), C₃-6 cycloalkyl or halo.

6. Compounds according to any one of the preceding claims, wherein

R² represents hydrogen, Ci_-4 alkyl [optionally substituted by one or more atoms selected from fluoro, -CN, -N(R^)R*², -N(R^)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9,-OR^y10, -S(O)_m-R^y11, -S(O)₂O-R^y12,

-S(O)₂N(R^y13)R^y14 and -C(O)R^y15],

C₃-6 cycloalkyl, halo or -0-Ci_-3 alkyl (optionally substituted by one or more fluoro atoms).

7. Compounds according to any one of the preceding claims, wherein:

R³, R^3a and R⁴ independently represent hydrogen, Ci_-3 alkyl (optionally substituted by one or more fluoro atoms) or halo.

8. Compounds according to any one of the preceding claims, wherein

-C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and/or -C(O)R^y15; or Ci_-7 alkyl optionally substituted by one or more substituents selected from fluoro, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and/or -C(O)R^y15; or aryl, heteroaryl (which latter two groups are optionally substituted by one or more substituents selected from -0-Ci_-3 alkyl, -CN, halo and Ci_-2 alkyl optionally substituted by one or more fluoro atoms); or any two R⁹ groups may be linked together as defined in claim 1.

9. Compounds according to any one of the preceding claims, wherein

R⁸ represents hydrogen, halo, Ci_-3 alkyl [optionally substituted by one or more substituents selected from fluoro, -OR^y1°, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, and -C(O)N(R^y8)R^y9] or -OR^y10.

10. Compounds according to any one of the preceding claims, wherein R⁶ represents hydrogen or d-6 alkyl optionally substituted by one or more substituents selected from fluoro, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)₂R^y11 and a 4- to 6-membered heterocycloalkyl group (containing two or one hetero- atom(s) selected from oxygen and nitrogen).

1 1. Compound according to any one of the preceding claims, wherein

12. Compounds according to any one of the preceding claims, wherein R^y4, R^, R^y11 and R^y15 independently represent Ci_-4 alkyl.

13. Compound according to any one of the preceding claims, wherein

A represents C_M2 linear or branched alkyl, aryl, heteroaryl, 5- or 6-membered heterocycloalkyl; or C_3-i₀ cycloalkyl, all of which groups are optionally substituted by one or more substituents selected from R⁹.

14. Compound according to any one of the preceding claims, wherein

A represents C_4-I2 linear or branched alkyl, aryl, heteroaryl, 5- or 6-membered heterocycloalkyl; or C_3-7 cycloalkyl (all of which groups are optionally substituted by one or more substituents selected from R⁹); or arylmethylene, heteroarylmethylene [which latter two groups are optionally substituted by one or more groups selected from Ci_-7 alkyl (optionally substituted by one or more substituents selected from fluoro and -OR^*2), halo, -CN and/or -0-Ci_-7 alkyl (optionally substituted by one or more fluoro atoms)].

15. Compounds of formula Ia, Ib, Ic or Id

in which R¹ represents Ci_-3 alkyl (optionally substituted by one or more fluoro atoms),

C₃-6 cycloalkyl, fluoro, chloro, bromo;

R³, R^3a and R⁴ independently represent hydrogen, fluoro, chloro, bromo, Ci_-3 alkyl (optionally substituted by one or more fluoro atoms);

R⁶ represents hydrogen or Ci_6 alkyl optionally substituted by one or more substituents selected from fluoro, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)₂R^y11 and a 4- to 6-membered heterocycloalkyl group (containing two or one heteroatom(s) selected from oxygen and nitrogen);

R⁸ independently represents hydrogen, fluoro, chloro, bromo, -OR^y1° or Ci_-3 alkyl

(optionally substituted by one or more fluoro atoms);

A represents phenyl, pyridyl, 5- or 6-membered heterocycloalkyl, C_3-7 cycloalkyl,

C_5-8 linear or branched alkyl (all of which groups are optionally substituted by one or more substituents selected from R⁹); or benzyl, pyridylmethylene [which latter two groups are optionally substituted by one or more groups selected from Ci_-7 alkyl (optionally substituted by one or more substituents selected from fluoro and -OR^*2), halo, -CN and/or

-O-Ci_-7 alkyl (optionally substituted by one or more fluoro atoms)];

R⁹ represents on each occasion when used herein: halo, -CN, -N(R^y1)R^y2,

-N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and/or -C(O)R^y15, Ci_-6 alkyl optionally substituted by one or more substituents selected from halo, -CN, -N(R^y1)R^y2, -N(R^y3)-C(O)-R^y4, -N(R^y5)-S(O)₂-R^y6, -C(O)OR^y7, -C(O)N(R^y8)R^y9, -OR^y1°, -S(O)_m-R^y11, -S(O)₂O-R^y12, -S(O)₂N(R^y13)R^y14 and/or -C(O)R^y15; or any two R⁹ substituents, when attached to the adjacent atoms of the A group and, in the case where the R⁹ substituents are attached to a non-aromatic A group, when attached to the same atoms, may be linked together to form, together with the essential atoms of the A group to which the relevant R⁹ substituents are necessarily attached, a further

3- to 8-membered ring, optionally containing a further one or two heteroatoms, and which further ring optionally contains one or two unsaturations and which is optionally substituted by one or more Ci_-3 alkyl and/or =0 substituents; and the substituents R^*2, R^y1, R*², R^y3, R^y4, R^y5, R^y6, R^y7, R^y8, R^y9, R^y1°, R^y11, R^y12, R^y13, R^y14 and

R^y15 have the meaning as defined in claims 1 , 11 or 12.

16. Compounds compounds of formula Ie, If, Ig or Ih:

If

R² represents hydrogen, chloro, bromo, fluoro, Ci_-3- alkyl (which latter alkyl group is optionally substituted by one or more fluoro atoms);

R ^■ ₅3 , o R 3a and R independently represent hydrogen, chloro, bromo, fluoro, Ci_-3- alkyl (which latter alkyl group is optionally substituted by one or more fluoro atoms);

represents phenyl, 2-pyridyl, 5- or 6-membered heterocycloalkyl, C_3-7 cycloalkyl, C_5-3 linear or branched alkyl (all of which groups are optionally substituted by one or more substituents selected from R⁹); or benzyl, pyridin-2-yl-methylene [which latter two groups are optionally substituted by one or more groups selected from Ci_-7 alkyl (optionally substituted by one or more substituents selected from fluoro and -OR¹*²), halo, -CN and/or -0-Ci_-7 alkyl (optionally substituted by one or more fluoro atoms)];

17. Compounds according to any one of the preceding claims, namely

2-(2,6-Dichloro-phenylamino)-3H-imidazo[4,5-c]pyridine-6-carboxylic acid 83

(4,4-dimethyl-cyclohexyl)-amide;

2-(2,6-Dichloro-phenylamino)-3H-benzimidazole-5-carboxylic acid cyclohexylamide;

2-(2-Chloro-6-fluoro-phenylamino)-3-methyl-N-(4-trifluoromethyl-pyridin-2- yl)-3-H-imidazo[4,5-c]pyridine-6-carboxamide; N-(4-Bromophenyl)-2-(2-chloro-6-fluoro-phenylamino)-3-methyl-3H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-3-methyl-N-(4-trifluoromethyl-pyridin-2-yl)- 364

3H-imidazo[4,5-c]pyridine-6-carboxamide;

N-(4-Bromophenyl)-2-(2,6-dichlorophenylamino)-3-methyl-3H-imidazo[4,5- 365 c]pyridine-6-carboxamide;

2-(3,5-Dichloropyridin-4-ylamino)-N-(4-trifluoromethyl-pyridin-2-yl)-1 H- 366 imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Chloro-6-fluorophenylamino)-N-(4,4-dimethylcyclohexyl)-3-methyl- ooy

3H-imidazo[4,5-c]pyridine-6-carboxamide;

N-(4-tert.-Butyl-pyridin-2-yl)-2-(2,6-dichlorophenylamino)-1 H-imidazo[4,5- oy o c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(4,4-dimethylcyclohexyl)-3-methyl-3H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(4-trifluoromethyl-pyridin-2-yl)-1 H- 414 imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Chloro-6-fluoro-phenylamino)-3-methyl-N-(spiro[2.5]octan-6-yl)-3H- 417 imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-3-methyl-N-((1 r,4r)-4-trifluoromethyl- cyclohexyl)-3H-imidazo[4,5-c]pyridine-6-carboxamide;

⁴²⁵ 2-(2,6-Dichlorophenylamino)-3-methyl-N-(spiro[2.5]octan-6-yl)-3H- imidazo[4,5-c]pyridine-6-carboxamide;

N-(4-Chloro-5-methyl-pyridin-2-yl)-2-(2,6-dichlorophenylamino)-1 H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Chloro-6-fluorophenylamino)-3-methyl-N-((1 r,4r)-4-trifluoromethyl- cyclohexyl)-3H-imidazo[4,5-c]pyridine-6-carboxamide;

N-(4-(Cyclopentyloxy)pyridin-2-yl)-2-(2,6-dichlorophenylamino)-1 H- 440 imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Chloro-6-fluorophenylamino)-N-cyclohexyl-3-methyl-3H-imidazo[4,5- 444 c]pyridine-6-carboxamide;

N-(4-Cyclopropylpyridin-2-yl)-2-(2,6-dichlorophenylamino)-1 H-imidazo[4,5- 445 c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(spiro[2.5]octan-6-yl)-1 H-imidazo[4,5- 451 c]pyridine-6-carboxamide;

N-(4-Cyclopropylpyridin-2-yl)-2-(2,6-dichlorophenylamino)-3-methyl-3H- 462 imidazo[4,5-c]pyridine-6-carboxamide

N-(4-Bromophenyl)-2-(2,6-dichlorophenylamino)-3-methyl-3H-imidazo[4,5- 463 b]pyridine-6-carboxamide;

N-(4-Bromophenyl)-2-(3,5-dichloropyridin-4-ylamino)-3-methyl-3H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(2-trifluoromethyl-benzyl)-1 H-imidazo[4,5- 477 c]pyridine-6-carboxamide;

2-(3,5-Dichloropyridin-4-ylamino)-N-(4,4-dimethylcyclohexyl)-3-methyl-3H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-((1 r,4r)-4-trifluoromethyl-cyclohexyl)-1 H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloropyridin-4-ylamino)-3-methyl-N-(4-trifluoromethyl-pyridin-2- yl)-3H-imidazo[4,5-c]pyridine-6-carboxamide;

N-Cyclohexyl-2-(2,6-dichlorophenylamino)-3-methyl-3H-imidazo[4,5- c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(4-(tetrahydrofuran-3-yl-oxy)-pyridin-2-yl)- 495

1 H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Chloro-6-fluorophenylamino)-3-methyl-N-((3-trifluoromethyl-pyridin-2- yl)-methyl)-3H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-((3-trifluoromethyl-pyridin-2-yl)methyl)-1 H- ouy imidazo[4,5-c]pyridine-6-carboxamide; 2-(3,5-Dichloropyridin-4-ylamino)-3-methyl-N-((1 r,4r)-4-trifluoromethyl- cyclohexyl)-3H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Chloro-6-fluorophenylamino)-N-((3-cyclopropylpyridin-2-yl)-methyl)-3- 522 methyl-3H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-3-methyl-N-((3-trifluoromethyl-pyridin-2-yl)- 528 methyl)-3H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloropyridin-4-ylamino)-3-methyl-N-((3-trifluoromethyl-pyridin-2- 550 yl)-methyl)-3H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(4,6-dimethyl-pyridin-2-yl)-1 H-imidazo[4,5- 716 c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(4,5-dimethyl-pyridin-2-yl)-1 H-imidazo[4,5- c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(5-chloro-4-methyl-pyridin-2-yl)-1 H- 718 imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(5-fluoro-4-methyl-pyridin-2-yl)-1 H- ( i y imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(5-trifluoromethyl-pyridin-2-yl)-1 H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2_!6-Dichlorophenylamino)-N-(2_!2_!3_!3_!4_!4_!5_!5_!6_!6_!7_!7_!8_!8,8- pentadecafluoro-octyl)-1 H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(4-bromo-phenyl)-1 H-imidazo[4,5- c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(6-methyl-4-trifluoromethyl-pyridin-2-yl)-1 H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-3-methyl-N-(4,5-dimethyl-pyridin-2-yl)-3H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-N-(5-cyclopropyl-pyridin-2-yl)-1 H-imidazo[4,5- c]pyridine-6-carboxamide;

2-(2,6-Dichlorophenylamino)-3-methyl-N-(5-chloro-4-methyl-pyridin-2-yl)- 726

3H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Fluoro-6-trifluoromethyl-phenylamino)-N-(4-trifluoromethyl-pyridin-2- yl)-1 H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Chloro-6-trifluoromethyl-phenylamino)-N-(4-trifluoromethyl-pyridin-2- 728 yl)-1 H-imidazo[4,5-c]pyridine-6-carboxamide;

729 2-(2-Chloro-6-fluoro-phenylamino)-N-(4-trifluoromethyl-pyridin-2-yl)-1 H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2,6-Dichloro-4-fluoro-phenylamino)-N-(4-trifluoromethyl-pyridin-2-yl)-1 H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(2-Chloro-4,6-difluoro-phenylamino)-N-(4-trifluoromethyl-pyridin-2-yl)-1 H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloro-pyridin-4-yl-amino)-N-(4-bromo-phenyl)-1 H-imidazo[4,5- c]pyridine-6-carboxamide;

2-(3,5-Dichloro-pyridin-4-yl-amino)-N-(6-methyl-4-trifluoromethyl-pyridin-2- yl)-1 H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(4-Trifluoromethyl-pyridin-3-ylamino)-3-methyl-N-(4-trifluoromethyl- pyridin-2-yl)-3H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloro-pyridin-4-yl-amino)-N-(4-cyclopropyl-5-methyl-pyridin-2-yl)- 735

1 H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloro-pyridin-4-yl-amino)-N-(6-chloro-4-trifluoromethyl-pyridin-2- 736 yl)-1 H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloro-pyridin-4-yl-amino)-N-(5-isopropyl-pyridin-2-yl)-1 H- imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloro-pyridin-4-yl-amino)-N-(4-cyclobutyl-pyridin-2-yl)-1 H- 738 imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloro-pyridin-4-yl-amino)-N-(6-dimethylamino-4-trifluoromethyl- pyridin-2-yl)-1 H-imidazo[4,5-c]pyridine-6-carboxamide;

2-(3,5-Dichloro-pyridin-4-yl-amino)-N-(6-cyclopropyl-4-trifluoromethyl- 740 pyridin-2-yl)-1 H-imidazo[4,5-c]pyridine-6-carboxamide.

18. Compounds according to any one of the preceding claims or a pharmaceutically- acceptable salt thereof, for use as a pharmaceutical.

19. Compounds according to any one of the preceding claims or a pharmaceutically- acceptable salt thereof, for use in the treatment of asthma, chronic obstructive pulmonary disease, pulmonary fibrosis, inflammatory bowel disease, irritable bowel syndrome, pain, inflammatory pain, fever, migraine, headache, low back pain, fibromyalgia, a myofascial disorder, a viral infection, a bacterial infection, a fungal infection, dysmenorrhea, a burn, a surgical or dental procedure, a malignancy, hyperprostaglandin E syndrome, classic Bartter syndrome, atherosclerosis, gout, arthritis, osteoarthritis, juvenile arthritis, rheumatoid arthritis, rheumatic fever, ankylosing spondylitis, Hodgkin's disease, systemic lupus erythematosus, vasculitis, pancreatitis, nephritis, bursitis, conjunctivitis, iritis, scleritis, uveitis, wound healing, dermatitis, eczema, psoriasis, stroke, diabetes mellitus, a neurodegenerative disorder, an autoimmune disease, an allergic disorder, rhinitis, an ulcer, coronary heart disease, sarcoidosis, any other disease with an inflammatory component, osteoporosis, osteoarthritis, Paget's disease, a periodontal disease or a cancer.