WO2010011884A3 - Novel nucleic acid-based molecular probes - Google Patents

Novel nucleic acid-based molecular probes Download PDF

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Publication number
WO2010011884A3
WO2010011884A3 PCT/US2009/051632 US2009051632W WO2010011884A3 WO 2010011884 A3 WO2010011884 A3 WO 2010011884A3 US 2009051632 W US2009051632 W US 2009051632W WO 2010011884 A3 WO2010011884 A3 WO 2010011884A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
aptamer
based molecular
novel nucleic
molecular probes
Prior art date
Application number
PCT/US2009/051632
Other languages
French (fr)
Other versions
WO2010011884A2 (en
Inventor
Weihong Tan
Zhiwen Tang
Original Assignee
University Of Florida Research Foundation, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Florida Research Foundation, Inc. filed Critical University Of Florida Research Foundation, Inc.
Publication of WO2010011884A2 publication Critical patent/WO2010011884A2/en
Publication of WO2010011884A3 publication Critical patent/WO2010011884A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3515—Lipophilic moiety, e.g. cholesterol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Abstract

The subject invention provides a novel nucleic acid-based molecular probe design employing intramolecular signal transduction. In an embodiment specifically exemplified herein, the probe comprises four elements: an aptamer; a short, partially complementary DNA sequence; a PEG linker conjugating the aptamer with the short DNA strand; and a luminescent molecule attached to a terminus of the aptamer and a terminus of the DNA sequence.
PCT/US2009/051632 2008-07-25 2009-07-24 Novel nucleic acid-based molecular probes WO2010011884A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13592708P 2008-07-25 2008-07-25
US61/135,927 2008-07-25

Publications (2)

Publication Number Publication Date
WO2010011884A2 WO2010011884A2 (en) 2010-01-28
WO2010011884A3 true WO2010011884A3 (en) 2010-05-06

Family

ID=41570876

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/051632 WO2010011884A2 (en) 2008-07-25 2009-07-24 Novel nucleic acid-based molecular probes

Country Status (1)

Country Link
WO (1) WO2010011884A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101812528B (en) * 2010-04-26 2012-07-04 湖南大学 Switch mode aptamer probe and application thereof in tumor living cell and vital detection
US10077474B2 (en) 2012-05-29 2018-09-18 Abbott Molecular, Inc. Method of designing primers, method of detecting single nucleotide polymorphisms (SNPs), method of distinguishing SNPs, and related primers, detectable oligonucleotides, and kits
CN102735669A (en) * 2012-07-02 2012-10-17 湖南大学 Nucleic acid aptamer molecular beacon probe for detection of platelet-derived growth factor
WO2020188323A1 (en) * 2019-03-18 2020-09-24 Khashayar Karimian Mate and separate: a convenient and general method for the separation and purification of target molecules from biological media by phase transition of pegylated recognition agents.
CN110161240B (en) * 2019-05-29 2020-10-09 福州大学 Pseudomonas aeruginosa detection method based on aptamer fluorescence sensing

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US20050176023A1 (en) * 2003-08-01 2005-08-11 Dynal Biotech Inc. Self-hybridizing multiple target nucleic acid probes and methods of use
US20050214797A1 (en) * 2003-10-28 2005-09-29 Epoch Biosciences, Inc Fluorescent probes for DNA detection by hybridization with improved sensitivity and low background
US20060088856A1 (en) * 2004-08-13 2006-04-27 Stratagene California Dual labeled fluorescent probes
US20060194240A1 (en) * 2005-02-28 2006-08-31 Gen-Probe Incorporated Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe
US20060292592A1 (en) * 2005-02-09 2006-12-28 Stratagene California Key probe compositions and methods for polynucleotide detection

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US20050176023A1 (en) * 2003-08-01 2005-08-11 Dynal Biotech Inc. Self-hybridizing multiple target nucleic acid probes and methods of use
US20050214797A1 (en) * 2003-10-28 2005-09-29 Epoch Biosciences, Inc Fluorescent probes for DNA detection by hybridization with improved sensitivity and low background
US20060088856A1 (en) * 2004-08-13 2006-04-27 Stratagene California Dual labeled fluorescent probes
US20060292592A1 (en) * 2005-02-09 2006-12-28 Stratagene California Key probe compositions and methods for polynucleotide detection
US20060194240A1 (en) * 2005-02-28 2006-08-31 Gen-Probe Incorporated Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe

Also Published As

Publication number Publication date
WO2010011884A2 (en) 2010-01-28

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