WO2010006513A1 - Portable diagnostic equipment for precancerous lesion of cervical cancer - Google Patents

Portable diagnostic equipment for precancerous lesion of cervical cancer Download PDF

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Publication number
WO2010006513A1
WO2010006513A1 PCT/CN2009/070098 CN2009070098W WO2010006513A1 WO 2010006513 A1 WO2010006513 A1 WO 2010006513A1 CN 2009070098 W CN2009070098 W CN 2009070098W WO 2010006513 A1 WO2010006513 A1 WO 2010006513A1
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WO
WIPO (PCT)
Prior art keywords
light source
light
cervix
cervical
detection system
Prior art date
Application number
PCT/CN2009/070098
Other languages
French (fr)
Chinese (zh)
Inventor
曾堃
虞震芬
Original Assignee
Zeng Kun
Yu Zhen Fen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CN 200810131630 priority Critical patent/CN101322644B/en
Priority to CN200810131630.7 priority
Application filed by Zeng Kun, Yu Zhen Fen filed Critical Zeng Kun
Priority claimed from US13/054,415 external-priority patent/US20110184272A1/en
Publication of WO2010006513A1 publication Critical patent/WO2010006513A1/en

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Detecting, measuring or recording for diagnostic purposes; Identification of persons
    • A61B5/0059Detecting, measuring or recording for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071Detecting, measuring or recording for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/012Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor characterised by internal passages or accessories therefor
    • A61B1/015Control of fluid supply or evacuation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/04Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
    • A61B1/043Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances for fluorescence imaging
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/06Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
    • A61B1/0607Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements for annular illumination
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/06Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
    • A61B1/0638Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements providing two or more wavelengths
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/06Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
    • A61B1/0661Endoscope light sources
    • A61B1/0684Endoscope light sources using light emitting diodes [LED]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/303Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for the vagina, i.e. vaginoscopes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Detecting, measuring or recording for diagnostic purposes; Identification of persons
    • A61B5/0059Detecting, measuring or recording for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082Detecting, measuring or recording for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084Detecting, measuring or recording for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells

Abstract

A portable diagnostic equipment (600) for precancerous lesion of cervical cancer comprises an inherent fluorescence detection system (602) for cervical cancer, a cervical acetic acid coloring detection system (604), and an image acquisition system (606). The inherent fluorescence detection system (602) includes a light source (620) and a light source switch (622). The light source (620) includes an excited light source that supplies fluorescence and a cold light source that supplies white light, and the excited light source and the cold light source are all installed in a light-reflecting cup (624) with a given focus. The cervical acetic acid coloring detection system (604) comprises an acetic acid pool (640), a spray pipe (642) connected to the acetic acid pool (640), a mobile device (644) of spray pipe and a spray switch (646). The image acquisition system (606) includes a weak light CCD (660), and the weak light CCD (660) is installed in the center of the light-reflecting cup (624). The image acquisition system (606) is connected to an exterior image processing system (700), and said exterior image processing system (700) processes the images acquired by the image acquisition system (606).

Description

The portable diagnostic device cervical precancerous lesions Field

The present invention relates to the diagnosis of precancerous lesions, and more particularly, rapid screening or diagnostic device detects standard precancerous cervical relates. Background technique

One of the main reasons of high mortality of cancer in the world, is no major breakthrough diagnostics, still remain in the gross morphology of diagnostic methods. The doctor's experience, colposcopy, endoscopy, B ultrasound, CT, MRI and other gross morphological diagnostic methods, diagnostic methods for such micro less than 0.5cm cancer diagnosis is still difficult to identify. And because pathogens epithelial cancers originate in epithelial (i.e. mucous membrane), the break because no skin layer on all of the features (mucous membrane) is formed at the end of cancer, so called precancerous lesions. In Example cervical precancerous lesions (referred CIN, cervical intraepithelial neoplasia), shown in Figure 1, in CIN I CIN II and stage cancer occurs pathogenic mucosal 1/3 parts, the epithelium (mucous membrane) surface without any traces of lesions, so the use of gross morphological diagnosis is unable to detect the exact precancerous lesions. CIN is the detection and treatment of the underlying implementation of appropriate treatment success, but also the only way to develop its blockade of cervical cancer.

Many currently used in the diagnosis of cervical cancer worldwide methods and apparatus, it is widely used colposcopy (Colposcope) principle which is actually a magnifying glass for viewing the cervix epithelium (mucous membrane) surface color contour changes, and changes in blood vessel . CIN and biochemical changes in the cells and the epithelium (mucous membrane), is difficult to exhibit valuable information epithelium (mucous membrane) layer, so colposcopy for cervical cancer (preinvasive cancer) or carcinoma in situ (intraepithelial cancer) detection and diagnosis has value, and the detection rate of CIN is low.

After acetic acid was visually observed coloring VIA are valuable in detecting CIN, transcervical acetate smear can show and poorly differentiated epithelial metaplasia, showing they showed white patches, these white plaques might be CIN lesions but CIN its positive rate of about 40%.

SUMMARY

The present invention aims to provide an efficient detection of CIN lesions, cervical cancer and precancerous lesions have portable diagnostic device for detecting a low-cost, integrated system of the intrinsic fluorescence image and cervical colored acetate system diagnostic device integrally.

According to an aspect of the present invention, it discloses a portable cervical precancerous lesion diagnostic apparatus, comprising:

Cervical intrinsic fluorescence detection system, the intrinsic fluorescence of cervical cancer detection system comprises a light source and a light source for opening and closing a control switch, comprising providing said light source and fluorescence excitation light source providing cool white light, the excitation light source and the cold source They are installed specific focal length reflective bowl, the bowl reflector focusing light source provided to the cervix; cervical acetic acid coloring detection system, which detection system comprises a coloring cervix acetic acid pool, the pool is connected to the spray tube acetate, moving means for moving the spray tube position of the spray tube, the spray switch and a control for opening and closing the spray tube; the intrinsic fluorescence of cervical cancer detection system sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix; the sub cervical acetic acid coloring detection system to spray the cervix, after a period of cold light source by the sequential use for cervical cancer detection system and the intrinsic fluorescence excitation light source providing excitation light and white light cervix. The apparatus can be used for rapid screening.

According to another aspect of the present invention, there is provided a portable cervical precancerous lesion diagnostic apparatus, comprising: a cervical intrinsic fluorescence detection system, the intrinsic fluorescence of cervical cancer detection system comprises a light source and a control switch for opening and closing a light source, the light source includes providing a fluorescence excitation light source and the cold light source to provide white light, the excitation light source and the cold light source are mounted on a particular focal length reflective bowl, the bowl reflector to focus light to a light source provided in the cervix; cervical acetate coloring detection system, which detection system comprises a coloring cervix acetic acid pool, the pool is connected to the acetic acid spray tube for spraying the spray tube moving means moves the position of the tube, the spray switch and a control for opening and closing the spray tube; image acquisition system, the the image acquisition system includes a weak light CCD, the CCD is mounted in the low light reflective bowl center position, collecting light reflected from the cervix; the image acquisition system connected to an external image processing system, the external image processing system processes the the image acquisition system of the image; the intrinsic fluorescence detection of cervical cancer And successively cold light system using a white light and the excitation light source providing excitation light irradiation cervix, cervical image acquisition system white light and the excitation light and the reflected image provided to the external graphics processing system; acetate colored detection system according to the cervix acetic acid spray to the cervix, after a period of use of the intrinsic fluorescence of cervical cancer detection system and the excitation light source sequentially provides cold white light and the excitation light irradiating the cervix, the image acquisition system through the cervix after the spraying of acetic acid and the white the excitation light and the reflected image provided to the external graphics processing system. The apparatus may be used for benchmarking.

The portable cervical precancerous lesion diagnostic device is fixed on a bracket-type pistol, the pistol-shaped bracket includes a horizontal portion and a handle portion, said rear end connected to an upper end of the horizontal portion of the handle portion, wherein the front end of the reflector bowl disposed horizontal portion, said light source arranged at equal intervals in a ring form on the reflective bowl; acetate is placed inside the cell handle portion, said spray pipe from the acetate pool extending inside the handle portion upward, continues to extend inside the horizontal part along the underside of the horizontal portion of the spray tube through a reversing device to the front end of the horizontal portion; mobile device of the spray tube the mobile device may be slidably mounted on the horizontal portion of the spray pipe extends to outside the mobile device is connected to the horizontal portion and located inside the horizontal portion of the spray tube, the spray tube of the spray can drive projecting front end of the horizontal tube portion; a light switch mounted on the handle portion, said light source comprises a switch for controlling the excitation light source opening Closing the switch and control the excitation light source cold light source for opening and closing the cold switch; the spray switch mounted on the handle portion.

The wavelength of the excitation light is 360 ~ 440nm. The excitation light source comprising: a laser generating light of 360 ~ 440nm, light is generated 360 ~ 440nm LED generates light 360 ~ 440nm high pressure mercury lamp or a xenon lamp to generate light of 360 ~ 440nm. The excitation light source through a quartz optical fiber, a fiber or a liquid light guide the common fiber to the reflector bowl.

Said reflector bowl surface coated with an ultraviolet reflective hard or soft ROBOT ROBOT.

The external graphics processing system comprising: an interface circuit, connected to the image acquisition system of weak light CCD; the CPU, connected to the interface circuit, receiving the image captured by the weak light CCD interface circuit; Image Analysis means, coupled to said CPU, said CPU analyzing the image transmitted; image processing means connected to the image analysis device, the image analysis processing; storage means, connected to the image processing apparatus, said storage and the image processing result; output means, and outputs the result of the image processing.

According to another aspect of the present invention, there is provided a portable cervical precancerous lesion diagnostic apparatus, comprising: a cervical intrinsic fluorescence detection system, the intrinsic fluorescence of cervical cancer detection system comprises a light source and a control switch for opening and closing a light source, the light source includes providing a fluorescence excitation light source and the cold light source to provide white light, the excitation light source and the cold light source are mounted on a particular focal length reflective bowl, the bowl reflecting the light source is focused to provide the cervix; for application acetic stick to cotton or cotton cervix; image acquisition system, the image acquisition system includes a weak light CCD, the CCD is mounted in the low light reflective bowl center position, collecting light reflected from the cervix; the image acquisition system connected to an external image processing system, the external image processing system for processing the image of the image acquisition system; and the intrinsic fluorescence of cervical cancer detection system sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix, image acquisition cervical white light collection system and the excitation light and the reflected image provided to the outer The image processing system; after the tampon applicator or cotton stick cervix acetate to wait a period of time used by the intrinsic fluorescence of cervical cancer detection system and the excitation light source sequentially provides cold white light and the excitation light irradiating the cervix, image acquisition after capturing the cervix acetate system applied to the white light and the excitation light and the reflected image provided to the external graphics processing system. The apparatus may be used for benchmarking.

All of the apparatus, the excitation light excitation light source is LED, the white LED is a cold light source, the excitation light from the LED and white LED are respectively disposed within the portable isolating cervical precancerous lesion diagnostic apparatus darkroom , input a bifurcated liquid or quartz fiber excitation light are respectively connected to the LED and white LED, the output of the bifurcated optical fiber is quartz or a liquid metal or a plastic outer wrap.

According to another aspect of the present invention, there is provided a portable cervical precancerous lesion diagnostic apparatus, comprising: a cervical intrinsic fluorescence detection system, the intrinsic fluorescence of cervical cancer detection system comprises a light source and a control switch for opening and closing a light source, the light source includes providing a fluorescence excitation light source and the cold light source to provide white light, the excitation light source and an input terminal connected to a cold light metal or plastic or a liquid coat wrapped quartz optical fiber, said silica fiber output end or direct liquid irradiating the cervix; cervical acetate colored detection system, which detection system comprises a cervical cell coloring acetic acid, acetic acid pool connected to the spray tube, the spray tube moving means for the spray tube position, and controlling the opening and closing of the spray tube spray after the cervix acetic acid coloring detection system to spray the cervix, wait for some time by the promoter; switch; the intrinsic fluorescence of cervical cancer detection system using a cold light source and sequentially provide white light and the excitation light irradiating excitation cervix cervical intrinsic fluorescence detection system using cold light source sequentially Excitation light source providing excitation light and white light cervix.

The present invention combines the intrinsic fluorescence image detection method and detection method of coloring cervical acetate, greatly improving the cervical precancerous lesions (CI N) disease detection rate can reach 80%, the basic method of the present invention based on the use of objective , less reliance on experience, even if the same primary physician can more accurately detected CI N. Further, the detection method of detecting low cost, high speed, detecting a patient requires only cost $ 2 detects only takes about five minutes. The portable sub invention cervical precancerous lesion diagnostic device may find more precancerous cervical disease, i.e., after maturation treatment which can block the development of cervical cancer direction, i.e. directly reduces the incidence of uterine cancer rate. BRIEF DESCRIPTION

The above and other features, nature, and advantages of the invention will become more apparent from the following description of embodiments in conjunction with the drawings and the accompanying drawings in which like reference numerals refer to like features, wherein:

FIG 1 discloses a measurable fluorescence spectra of biomolecules intraepithelial;

FIG 2 discloses a gradual process of epithelial cells;

FIG 3 discloses the formation and development of cervical cancer;

FIG 4 discloses a process of gradual cellular host abnormal early information on the surrounding environment of biochemical; FIG. 5 discloses the principle of fluorescence generated;

FIG 6 discloses a portable child present invention cervical precancerous lesion diagnostic apparatus configuration diagram of a first embodiment;

FIG 7 discloses a block diagram of an external image processing system according to the present invention;

FIG 8 discloses a portable child present invention cervical precancerous lesion diagnostic apparatus configuration diagram of a second embodiment;

Figures 9a and 9b present invention discloses a portable child's cervical precancerous lesion diagnostic apparatus configuration diagram of a third embodiment;

Figures 10a and 10b discloses a configuration diagram of a fourth embodiment of the present invention the sub-portable cervical precancerous lesion diagnostic apparatus. detailed description

85% of human tumor foci originated in the cortex, and cervical lesions is also originated from the mucosal epithelium, cervical epithelium located between 0.6mm ~ 1 .2mm, abnormal changes occurring within the epithelium was graded process, is to be expressed biochemical information, rather than the morphological form of expression, so the information can not cervical precancerous lesions physical means to obtain information, such as B-CIN, X-ray, CT, MRI, etc., of course, can not be directly observed gross morphological channel CIN Thus, morphological examination of the technique must be used which is different from the present invention employs detection technology based on biochemical, spectral technique is applied to the body early tumor detection. The present invention incorporates techniques LIF (Laser induced fluorescence), reference U.S. Patent No. (US 4,957,114), assigned to the above-mentioned U.S. Patent application of the applicant and incorporated herein by reference. LIF diagnostic methods and diagnostic criteria have been recognized. Measurable fluorescence spectra of biomolecules epithelial collagen comprises amino acids, and the coenzyme protease structure, and fat cell metabolism related porphyrin coenzymes adenine dinucleotide, flavin adenine dinucleotide (NADH) and flavin nucleic acid, tryptophan, collagen, anti-hormone dermatitis, elastin, intestinal fat pigment, acriflavine, porphyrin, which biomolecules have their own unique emission spectra shown in Figure 1, these biomolecules variations are to be expressed in the form of fluorescence spectrum.

Most malignant tumors derived from epithelial, and has gradually gradual process, of course, also from the gradient of cervical cancer in the mucosal epithelium. As shown in Figure 2, which shows a gradual process of epithelial cells. Said stage comprising a mutation phase CIN1, CIN2, CIN3 (carcinoma in situ), early invasive and invasive stages. Figure 3 illustrates the formation of cervical cancer and the development process. In conjunction with FIGS. 2 and 3 can be seen:

The earliest stages of cervical cancer lesions has not happened yet substantial, merely surroundings appeared adverse factors, these adverse factors include:

Dirty sex life caused by viruses, including these viruses HPV16, HPV18, HSV-2, HCMV, and so on;

Premature sexual life and benign lesions died;

Genetic and geographical factors.

The substantive stage disease did not occur, but these adverse factors that could cause disease, so mainly to prevent, eliminate adverse factors based. The stage has become the primary prevention stage. This stage corresponds to FIG 2 in the "normal epithelial" cell stage.

The second stage is the stage of gene mutations, is further sub-divided into three phases, namely: mutation (CINl), dysplasia (of CIN2) and carcinoma in situ (of CIN3), the above three gradation cell stages can be referred to FIG. 2 the CI N 1, CI N2, CI N3 stage. Gene mutation has undergone substantial stage of disease, but has not yet developed into cancer, so if you can perform effective diagnosis and treatment at this stage, can effectively block its development to become invasive cancer. Therefore, the real effective early diagnosis should be diagnosed in the second stage of the disease. However, mutations stage CI N 1, CI N2, CI N3 (carcinoma in situ) were graded mucosa, and there is no obvious morphological characteristics appear. So change detection of biochemical information becomes very important. The second stage has become secondary prevention stage.

With continued reference to FIGS. 2 and 3, it has formed the third stage cancer, i.e., early invasive and invasive carcinoma, corresponding to FIG. 2 in "early invasive" and "invasive" stage. At this time, cancer has been formed, the treatment becomes relatively difficult. The third stage, also known as tertiary prevention stage.

Seen by analyzing FIGS. 2 and 3, it is clear that the second critical phase of early diagnosis. The gradient of the cell during the second stage, although not obvious morphological, biochemical characteristics but the change is very clear. These features include abnormal biochemical processes of a host cell periphery gradual biochemical information early environment, as shown with reference to FIG. 4, the information comprising:

Blood vessels, hormones, carbohydrate metabolism nucleic acids, enzymes, proteins, gene mutation. Early information described above has its own typical frequency spectrum when irradiated with light of a certain frequency in the cervical mucous epithelium, under certain conditions, the electronic energy-absorbing transition to the higher energy level (i.e. excited state) if the electron excited directly the method of radiation state to a ground state, energy is released by the corresponding photon emission, which is generated fluorescence (fluorescence shown in FIG. 5 with reference to FIG generation principle). The basic principle is the detection of LIF, as shown in FIG. 5, the excitation light source 502 generates the excitation light 504 is irradiated through an optical fiber 506 to the mucosa 508, 510 electron transition occurs in the mucosa 508, produces a corresponding visible light image acquired by the naked eye or device 512 is observed.

Based on the above principle, by light from the cervical epithelium, it exhibits fluorescence characteristics of the mucosal epithelium from the cervix which represents whether the measured normal cervical mucous epithelium, benign lesions of CIN or cancer, it may be fluorescence spectrum curve Expression , a fluorescence image may also be expressed. In the present invention, the expression of the fluorescent image, the doctor visually with the naked eye or by screening diagnostic i.e. which can identify fluorescent color cervical mucous epithelium was observed in the display, characterized CI N detection rate can be detected in cervical epithelium CI N deeper lesions, detection of low cost, the cost per patient is only detecting a two yuan, speed, and detecting a patient only every five minutes. Further such additional CCD camera for automatic image recognition, storage and other functions by a computer, this method is suitable for hospital diagnostic applications.

Table shows the image discriminator CI N level standards. In the diagnostic fluorescence image on the basis of the present invention also introduces a colored image diagnosis acetate. Accordingly, the present invention is the core of the intrinsic fluorescence image and the diagnostic image diagnosis acetate combined colored body. Two kinds of separate diagnostic method embodiment diagnose CI N positive detection rate was 40%, the reason for the fluorescence diagnosis biochemical changes in the differential diagnosis of cervical epithelium as a basis, and the colored image diagnosis acetate (VIA) is displayed cervical epithelial layer (horny layer) changes in applicable parts are slightly different, but the lesion site in the mucosal epithelium and mucosal epithelium where substantially the outer half and half, causing the above-described method alone, diseased portion can not be detected. And the two kinds of combined diagnostic method, the basic structure may cover all cervical mucous epithelium, VIA responsible for the differential diagnosis of an outer epithelium intraepithelial fluorescence diagnosis is responsible, two kinds of complementary diagnostic method can therefore be precancerous cervical lesion detection rate to 70% to 80%. Normal cervical squamous epithelium with less protein in favor of biochemical information epithelial fluorescence detection, and when the metaplastic epithelium, poorly differentiated epithelial cell membrane, nucleus and cytoplasm contain more collagen and elastin, this time fluorescence diagnosis were covered by a surface layer protein fluorescence characteristics, can not be an important biochemical information showing the epithelium, such as NADH nicotinamide adenine dinucleotide (nicotinamide adeninedinucleotide) NADH antioxidants are considered free radical oxidation resistant protective effect . It causes cell destruction is reduced and favors the formation of cancer, and NADH fluorescence peak at about 470nm, 470nm may therefore be a peak value as possible according to the cancerous tissue or not. If cervical epithelial layer (horny layer) collagen and elastin are covered, that is not necessarily measured fluorescence is a fluorescent protein data, and important biochemical substances such as NADH in the epithelial etc. can not be displayed, this is the blind spot of fluorescence diagnosis. The VIA science is able to fill this blind spot. Two methods in combination with its overall positive rate of CIN substantial increase in data obtained by CIN screening large population confirmed the argument.

As shown in Figure 6, revealed the structure of a first embodiment of the present invention the sub-portable cervical precancerous lesion diagnostic apparatus. The portable cervical precancerous lesion diagnostic apparatus 600 comprises: the intrinsic fluorescence of cervical cancer detection system 602, the intrinsic fluorescence of cervical cancer detection system comprises a light source 620 and a light source control opening and closing of the switch 622, the light source includes providing a fluorescence excitation light source white and provide cold light, the excitation light source and the cold light reflector are mounted within the bowl 624 a particular focal length, the light source reflector bowl 624 is provided to focus the cervix. Wavelength of the excitation light is 360 ~ 440nm. Accordingly, the excitation light source may be employed the following: generating a laser light of 360 ~ 440nm, to produce LED 360 ~ 440nm light, generating high pressure mercury lamp 360 ~ 440nm light, a xenon lamp, or to generate light of 360 ~ 440nm. Wherein, when the excitation light source is a laser, a high-pressure mercury lamp, due to the larger volume of high pressure mercury lamp or a laser, so that they are placed in the portable cervical precancerous lesion diagnostic apparatus 600 outside, and through the quartz fiber, fiber or liquid common fiber directs light into the reflective bowl 624. Bowl 624 coated with reflective surface reflect ultraviolet hard or soft ROBOT ROBOT. Reflective bowl focal length 624 may be about 120mm.

The portable cervical precancerous lesion diagnostic apparatus 600 further comprises a detection system 604 cervical acetate coloring, the coloring cervix detection system 604 comprises acetic acid pool 640, connected to a pool acetate the spray tube 642, 642 for moving the spray tube position mobile device 644 of the spray tube, the spray tube 642 and a control opening and closing of the switches 646 spray.

The portable cervical precancerous lesion diagnostic apparatus 600 is fixed to the bracket a pistol, the pistol-type bracket 400 includes a horizontal portion and a handle portion 402, handle portion 402 and the rear end of the horizontal portion 400 of FIG. 7 with reference to FIG. upper end connection. Reflective bowl 624 placed on a horizontal front portion 400 of the light source 620 arranged at equal intervals in a ring shape on the reflective bowl 624. Pool acetate 640 disposed inside the handle portion 402, the spray tube 642 pool starting from acetate 640 extending inside the handle portion 402 upward, the inner side portion 400 of the lower horizontal portion of the spray tube through a transducer continues along the horizontal 404 extends toward the distal end of the horizontal portion. Means reversing the spray tube 404 is an annular structure surrounding the spray tube 642 of the spray pipe from the transducer to change the horizontal direction perpendicular to the extending direction of the coil device 404. The mobile device the spray tube 644 slidably mounted on the horizontal portion 400, the spray tube extending into the mobile device 644 outside of the horizontal portion 400 and inner portion 400 connected to the spray pipe 642 in the horizontal, the spray tube 644 can drive the mobile device the spray tube 642 front end portion 400 extends horizontally. Generally, the spray tube 642 may extend a distance of about 5cm horizontal portion 400 of the front end. Light switch 622 mounted on the handle portion 402, the light source comprises a switch 622 for controlling the excitation light excitation light source for opening and closing switch 621 and switch 623 controls the cold cold light source for opening and closing. In the embodiment shown in FIG. 6, the light source switch 622 employed to implement the bidirectional switch, the upper switch 621 as the excitation source, the lower portion of switch 623 as a cold source. Spray switch 646 is also mounted on the handle portion 402.

In the embodiment illustrated in Figure 6, further comprising an image acquisition system 606, the image acquisition system includes a weak light CCD 660, weak light CCD 660 mounted in the center position of the reflective bowl 624 for collecting light reflected from the cervix; the the image acquisition system 606 is connected to an external image processing system (refer to FIG. 7), the external image processing system for processing an image of the image acquisition system.

Referring shown in FIG. 7, the external image processing system 700 employed in this embodiment comprises: an interface circuit 702, connected to the image acquisition system 606 in the weak light CCD 660. CPU 704, connected to the interface circuit 702 receives weak light CCD 660 via the image acquisition interface circuit 702. The image analysis apparatus 706 is connected to the CPU 704, the image 704 analyzes the transmission CPU. The image processing apparatus 708, connected to the image analysis apparatus 706, the image analysis process. Storage device 710, connected to the image processing apparatus 708, and stores the image processing result. An output device 712, and the image processing result output, the output device 712 may include a display device 711 and the printing apparatus 713.

FIG operation of this embodiment shown in FIG. 6 as follows: First, fluorescence detection, cervical cancer intrinsic fluorescence detection system 602 sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix, image acquisition system 606 to collect cervical white the excitation light and the reflected image and supplied to the external graphics processing system 700. After acetic acid was detected, the detection system 604 cervical colored acetate Acetic acid was sprayed onto the cervix, after a specified time sequential use by the excitation light source and the cold light cervical intrinsic fluorescence detection system 602 provides white light and the excitation light irradiating the cervix, image acquisition system after collecting the cervix 606 by the spray of acetic acid and the white light reflected excitation light and images to the external image processing system 700. In a particular embodiment, during operation of cervical precancerous lesions of the portable diagnostic device as follows: after a good placement of the patient to be expanded is the female doctor distal portable cervical precancerous lesion diagnostic apparatus aligned horizontal expansion of the female portion about 30mm port, the switch is opened so that white light source cold start, the state through the cervix white CCD identification, diagnostics chamber closed all lights (i.e., dark room) to open the excitation light of the excitation light switch opens, by discriminating the cervix intrinsic fluorescence CCD lesions, and a reference table diagnostic criteria for determining the state of the patient's cervix. Close the excitation light and the white light source is turned on, the spray tube moving means moving the spray tube so that the end surfaces a certain distance to relocation (for example 5cm) and the alignment checked patients cervix, pressing the switch spray, the spray tube to a spraying checked patients cervix 0.5 ml acetic acid, to be 2-3 minutes if there is a CCD acetate white epithelium white light source in the differential diagnosis of a patient. By a CCD, can be seen directly on the display state of the cervix, CCD signals simultaneously received by a transmission interface circuit to the CPU, after the image analysis and image processing by a display or printer display or storage produce a diagnostic report .

Higher weak light CCD using a portable diagnostic device cervical precancerous lesions diagnostic accuracy, but relatively speaking, CCD and auxiliary equipment requires a large space, but also more complex, and therefore more suitable for use as a standard hospital examination.

FIG 8 discloses a configuration diagram of the present invention the sub-portable cervical precancerous lesion diagnostic apparatus according to the second embodiment. The first embodiment shown in FIG. 6 as compared with the second embodiment is distinguished in that the detection system is colored cervical acetate canceled, the direct use of isolated cotton applicator stick or cotton balls 604 acetic acid. In use, the first use of white light, after use cotton or cotton applicator stick for coloring acetate, and then irradiated with an excitation light. Embodiment shown in FIG. 8 embodiment still using weak light CCD, it is also more suitable as a standard hospital examination.

Structure of FIG. 9a and 9b of the present invention discloses a portable child's cervical precancerous lesion diagnostic apparatus according to the third embodiment. In this embodiment, the image acquisition system is canceled, the center position of the reflective bowl 924 which is not provided weak light CCD. In the case of the reflected light are observed by the naked eye doctor. The remaining structures were consistent with the embodiment illustrated in FIG. 6. While not using CCD detection accuracy will decrease somewhat, but can greatly increase the speed of detection, and the CCD and the image processing apparatus is not, the entire volume of the diagnostic apparatus is greatly reduced, increased flexibility, fluidity suitable for large scale rapid screening.

In the embodiment shown in Figure 9a and Figure 9b, the excitation of the excitation light from the LED light source 901, a cold white LED light source 903, the excitation light from the LED and white LED are respectively disposed within the portable isolated cervical precancerous lesion diagnostic apparatus the darkroom 902 and 904 (see FIG. 9b). Input terminal of a bifurcated liquid or quartz fiber 906 are connected to the excitation light LED and white LED 901

903. Quartz or bifurcated fiber output ends 906 of the liquid metal jacket or plastic wrap. In the example shown in Figure 9a and Figure 9b a solid, the use of a CCD is without. The above-described structure is applied to the first light source or the second embodiment has a CCD is also possible.

Figures 10a and 10b discloses a configuration diagram of a fourth embodiment of the present invention the sub-portable cervical precancerous lesion diagnostic apparatus. The fourth embodiment is a further variation of the third embodiment shown in Figures 9a and 9b. Among them, reflecting bowl was canceled. Embodiment illustrated in FIGS. 10a and 10b, the excitation light source for the excitation light from the LED 1001, 1003 cool white LED light source, excitation light from the LED and the white LED are respectively disposed within the portable isolated cervical precancerous lesion diagnostic apparatus darkroom 1002 and 1004 within a (see FIG. 10b). Input terminal of a bifurcated optical fiber or a liquid quartz 1006 LED 1001 are connected to the excitation light and white light LED 1003. Quartz or bifurcated fiber output ends 1006 of the liquid metal jacket or plastic wrap. Outside the distal end of bifurcated output liquid or quartz fiber 1006 extends out of the portable cervical precancerous lesion diagnostic apparatus, since the output terminal bifurcated quartz fiber or a liquid 1006 having a smaller diameter, the doctor easier using the naked eye observation. Greater flexibility in the embodiment shown in FIGS. 10a and 10b, equally suitable for mass rapid screening flowability.

While the present invention the above-described embodiments have been described, it should be noted that, in practice, the intrinsic fluorescence image detection device and the detection acetate coloring not necessarily be placed in the same bracket, separate arrangements are entirely possible. Alternatively, without using the apparatus of the form provided by the present invention, but a variety of other devices combined application intrinsic fluorescence imaging and cervical acetate colored detected it should be considered within the scope of the invention. Those skilled in the art, it is obvious that the present invention provides a high degree of accuracy is obtained by combining the intrinsic fluorescence image detection method for detecting colored acetate and cervical cancer detection means mucosa.

As described above, since the front most mucosal disease mucosal cancer lesions are close to the process, the device of the present invention may also be used to diagnose disease before other mucosal lesions.

Connection with the present invention, the intrinsic fluorescence image detection method and detection method for coloring acetate cervix, greatly improving the detection rate of cervical precancerous lesions (CI N) disease, may be about 80%, using the method of the present invention is substantially the objective basis, less reliance on experience, even if the same primary physician can more accurately detected CI N. Further, the detection method of detecting low cost, high speed, detecting a patient requires only cost $ 2 detects only takes about five minutes. The portable sub invention cervical precancerous lesion diagnostic device may find more precancerous cervical disease, i.e., after maturation treatment which can block the development of cervical cancer direction, i.e. directly reduces the incidence of uterine cancer rate.

Although the above description of the preferred embodiment of the present invention, but the present invention is not limited thereto. The skilled person in the art may make various changes and modifications on the basis of the above description. Various changes and modifications without departing from the spirit of the invention shall fall within the scope of the present invention. The scope of the invention by the appended claims is defined.

Claims

Rights request
A portable cervical precancerous lesion diagnostic apparatus, characterized by comprising: intrinsic fluorescence of cervical cancer detection system, the intrinsic fluorescence of cervical cancer detection system comprises a light source and a control switch for opening and closing a light source, said light source comprising providing a light source and a fluorescence excitation source providing cool white light, the excitation light source and the cold light source are mounted on a particular focal length reflective bowl, the bowl reflector provides the light source to focus the light cervix;
Cervical acetate coloring detection system, which detection system comprises a coloring cervix acetic acid pool, the pool is connected to the acetic acid spray tube for the spray tube means for moving the position of the spray tube, the spray tube and the spray opening and closing of the control switch;
The intrinsic fluorescence of cervical cancer detection system sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix; colored acid detection system of the cervix acetate to spray cervix, after a period of cervical cancer by the intrinsic fluorescence detection system sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix.
2. - precancerous cervical lesions seed portable diagnostic apparatus comprising: intrinsic fluorescence of cervical cancer detection system, the intrinsic fluorescence of cervical cancer detection system comprises a light source and a control switch for opening and closing a light source, said light source comprising providing a light source and a fluorescence excitation source providing cool white light, the excitation light source and the cold light source are mounted on a particular focal length reflective bowl, the bowl reflector provides the light source to focus the light cervix;
Cervical acetate coloring detection system, which detection system comprises a coloring cervix acetic acid pool, the pool is connected to the acetic acid spray tube for the spray tube means for moving the position of the spray tube, the spray tube and the spray opening and closing of the control switch;
Image acquisition system, the image acquisition system includes a weak light CCD, the CCD is mounted in the low light reflective bowl center position, collecting light reflected from the cervix; the image acquisition system connected to an external image processing system, the external the image processing system processes the image of the image acquisition system;
The intrinsic fluorescence of cervical cancer detection system sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix, cervical image acquisition system white light and the excitation light and the reflected image provided to the external graphics processing system; the said detection system cervix acetic acid coloring sprayed onto the cervix, after a period of use of the intrinsic fluorescence of cervical cancer detection system and the excitation light source sequentially provides cold white light and the excitation light irradiating the cervix, the image acquisition system via acetate after spraying of the cervix and the reflected image of the white light and the excitation light is supplied to the external image processing system.
3. The portable cervical precancerous lesion diagnostic apparatus according to claim 2, wherein said portable cervical precancerous lesion diagnostic device is fixed on a bracket-type pistol, the pistol includes a horizontal bracket portion and a handle portion, said rear end connected to an upper end of the horizontal portion of the handle portion, wherein,
The reflective bowl to the front of said horizontal portion, said light source arranged at equal intervals in a ring form on the reflective bowl;
The cells were placed inside the acetate handle portion, said spray pipe starts from the pool of acetate extends upward inside the handle portion of the spray tube through a reversing device continues along the lower rear portion of the horizontal extending toward the distal end side of the horizontal portion of the horizontal portion of the interior;
The mobile device of the spray tube slidably mounted on the horizontal portion of the spray pipe extends to outside the mobile device is connected to the horizontal portion and located inside the horizontal portion of the spray tube, the spray tube movement spray means can drive the front end of the horizontal pipe projecting portion; a light switch mounted on the handle portion, the light switch comprises a switch for controlling the excitation light excitation light source and the opening and closing of the opening and closing control of cold light source cold light switch;
The spray switch mounted on the handle portion.
The portable cervical precancerous lesion diagnostic apparatus as claimed in claim 2, wherein said excitation light wavelength of 360 ~ 440nm; said excitation light source comprises:
Generating a laser light of 360 ~ 440nm, to produce LED 360 ~ 440nm light, generating high pressure mercury lamp 360 ~ 440nm light, a xenon lamp, or to generate light of 360 ~ 440nm.
5. The portable cervical precancerous lesion diagnostic apparatus according to claim 4, wherein the excitation light source through a quartz optical fiber, a fiber or a liquid light guide the common fiber to the reflector bowl.
The portable cervical precancerous lesion diagnostic device as claimed in claim 2, wherein said reflective bowl surface coated with an ultraviolet reflective hard or soft ROBOT ROBOT.
The portable cervical precancerous lesion diagnostic device as claimed in claim 2, wherein the external graphics processing system comprising:
An interface circuit connected to the low light image of the CCD acquisition system;
The CPU, connected to the interface circuit, via the interface circuit receives the weak light CCD image acquisition;
Image analysis means, coupled to said CPU, said CPU analyzing the image transmitted; image processing means connected to the image analysis device, the image analysis processing; storage means, connected to the image processing apparatus, storing and the image processing result; output means, and outputs the result of the image processing.
A portable cervical precancerous lesion diagnostic apparatus, characterized by comprising: intrinsic fluorescence of cervical cancer detection system, the intrinsic fluorescence of cervical cancer detection system comprises a light source and a control switch for opening and closing a light source, said light source comprising providing a light source and a fluorescence excitation source providing cool white light, the excitation light source and the cold light source are mounted on a particular focal length reflective bowl, the bowl reflector provides the light source to focus the light cervix;
Acetic acid applied to the cervix for cotton or cotton stick;
Image acquisition system, the image acquisition system includes a weak light CCD, the CCD is mounted in the low light reflective bowl center position, collecting light reflected from the cervix; the image acquisition system connected to an external image processing system, the external the image processing system processes the image of the image acquisition system;
The intrinsic fluorescence of cervical cancer detection system sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix, cervical image acquisition system white light and the excitation light and the reflected image provided to the external graphics processing system; the said stick or cotton tampon applicator acetic acid to the cervix, wait a period of time by the sequential use of cold light cervical intrinsic fluorescence detection system and the excitation light source providing excitation light and white light illumination of the cervix, the image acquisition system via applicator acetate cervix after reflection image of the excitation light and the white light and supplied to the external image processing system.
9. The portable cervical precancerous lesion diagnostic apparatus 1, 2, or according to claim 8, characterized in that,
The excitation light excitation light source is LED, the white LED is a cold light source, the excitation light from the LED and white LED are respectively disposed within a dark room isolated from the portable cervical precancerous lesion diagnostic apparatus, a bifurcated or liquid input quartz fiber excitation light are respectively connected to the LED and white LED, the output of said bifurcated quartz optical fiber, or a liquid metal or a plastic outer wrap.
A portable cervical precancerous lesion diagnostic apparatus, characterized by comprising: intrinsic fluorescence of cervical cancer detection system, the intrinsic fluorescence of cervical cancer detection system comprises a light source and a control switch for opening and closing a light source, said light source comprising providing a light source and a fluorescence excitation source providing cool white light, the excitation light source and an input terminal connected to a cold light metal or plastic or a liquid coat wrapped quartz fiber, quartz or the output end of the optical fiber is directly irradiated liquid cervix ;
Cervical acetate coloring detection system, which detection system comprises a coloring cervix acetic acid pool, the pool is connected to the acetic acid spray tube for the spray tube means for moving the position of the spray tube, the spray tube and the spray opening and closing of the control switch;
The intrinsic fluorescence of cervical cancer detection system sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix; colored acid detection system of the cervix acetate to spray cervix, after a period of cervical cancer by the intrinsic fluorescence detection system sequentially using the light source provides excitation light source and a cold white light and the excitation light irradiating the cervix.
PCT/CN2009/070098 2008-06-13 2009-01-12 Portable diagnostic equipment for precancerous lesion of cervical cancer WO2010006513A1 (en)

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CN200810131630.7 2008-07-15

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US13/054,415 US20110184272A1 (en) 2008-06-13 2009-01-12 Portable Diagnostic Device for Precancerous Lesion of Cervical Cancer

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CN1302210A (en) * 1997-10-20 2001-07-04 得克萨斯系统大学评议会 Acetic acid as a signal enhancing contrast agent in fluorescence spectroscopy
CN1493250A (en) * 2002-10-31 2004-05-05 上海生标科技有限公司 Device using endoscope to diagnose precancer affection
CN1502305A (en) * 2002-11-22 2004-06-09 上海生标科技有限公司 Optical biopsy precancerous lesion image diagnostic instrument
US20080039720A1 (en) * 2006-06-05 2008-02-14 Forth Photonics Ltd. Methods for characterizing cancer and pre-cancer tissues
CN101322644A (en) * 2008-06-13 2008-12-17 堃 曾 Portable cervical cancer precancerosis diagnostic equipment

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5329938A (en) * 1983-02-17 1994-07-19 The Trylon Corporation Method for endoscopic examination of body cavity using chemilumine-scent light source
CN1302210A (en) * 1997-10-20 2001-07-04 得克萨斯系统大学评议会 Acetic acid as a signal enhancing contrast agent in fluorescence spectroscopy
CN1493250A (en) * 2002-10-31 2004-05-05 上海生标科技有限公司 Device using endoscope to diagnose precancer affection
CN1502305A (en) * 2002-11-22 2004-06-09 上海生标科技有限公司 Optical biopsy precancerous lesion image diagnostic instrument
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