WO2010006333A3 - Quantification of enzyme activity by mass spectrometry using immobilized substrates - Google Patents
Quantification of enzyme activity by mass spectrometry using immobilized substrates Download PDFInfo
- Publication number
- WO2010006333A3 WO2010006333A3 PCT/US2009/050407 US2009050407W WO2010006333A3 WO 2010006333 A3 WO2010006333 A3 WO 2010006333A3 US 2009050407 W US2009050407 W US 2009050407W WO 2010006333 A3 WO2010006333 A3 WO 2010006333A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- mass spectrometry
- quantification
- enzyme activity
- enzymes
- immobilized substrates
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The disclosure relates to methods of analyzing the enzymatic activity of enzymes in samples containing a plurality of enzymes, using mass spectrometry. Immobilized substrates are employed. Purified enzymes and enzymes from crude cell lysates can be analyzed using the disclosed methods.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09795285A EP2313518A2 (en) | 2008-07-11 | 2009-07-13 | Quantification of enzyme activity by mass spectrometry using immobilized substrates |
US13/003,731 US20110281289A1 (en) | 2008-07-11 | 2009-07-13 | Quantification of enzyme activity by mass spectrometry using immobilized substrates |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8001408P | 2008-07-11 | 2008-07-11 | |
US61/080,014 | 2008-07-11 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2010006333A2 WO2010006333A2 (en) | 2010-01-14 |
WO2010006333A3 true WO2010006333A3 (en) | 2011-05-05 |
Family
ID=41507773
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2009/050407 WO2010006333A2 (en) | 2008-07-11 | 2009-07-13 | Quantification of enzyme activity by mass spectrometry using immobilized substrates |
Country Status (3)
Country | Link |
---|---|
US (1) | US20110281289A1 (en) |
EP (1) | EP2313518A2 (en) |
WO (1) | WO2010006333A2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0906698D0 (en) | 2009-04-17 | 2009-06-03 | Queen Mary & Westfield College | Method for quantifying modified peptides |
GB201204278D0 (en) | 2012-03-09 | 2012-04-25 | Queen Mary & Westfield College | Method |
EP3474014B1 (en) | 2013-03-15 | 2020-06-17 | Expression Pathology, Inc. | Srm assay to indicate cancer therapy |
US10760113B2 (en) * | 2015-07-10 | 2020-09-01 | Regents Of The University Of Minnesota | Kinase activity detection methods |
JP7432529B2 (en) | 2018-05-30 | 2024-02-16 | プロメガ コーポレイション | Broad Spectrum Kinase Binders |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007127767A2 (en) * | 2006-04-28 | 2007-11-08 | Ludwig Institute For Cancer Research | Quantification of enzyme activity by mass spectrometry |
US20080076676A1 (en) * | 2006-02-27 | 2008-03-27 | Korea Advanced Institute Of Science And Technology | Novel construction of gold nanoparticle-based peptide chip, and assaying enzyme activity and inhibitor effect using secondary ion mass spectrometric analysis thereof |
-
2009
- 2009-07-13 US US13/003,731 patent/US20110281289A1/en not_active Abandoned
- 2009-07-13 WO PCT/US2009/050407 patent/WO2010006333A2/en active Application Filing
- 2009-07-13 EP EP09795285A patent/EP2313518A2/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080076676A1 (en) * | 2006-02-27 | 2008-03-27 | Korea Advanced Institute Of Science And Technology | Novel construction of gold nanoparticle-based peptide chip, and assaying enzyme activity and inhibitor effect using secondary ion mass spectrometric analysis thereof |
WO2007127767A2 (en) * | 2006-04-28 | 2007-11-08 | Ludwig Institute For Cancer Research | Quantification of enzyme activity by mass spectrometry |
Non-Patent Citations (3)
Title |
---|
GRÜNINGER-LEITCH F ET AL: "Identification of beta-secretase-like activity using a mass spectrometry-based assay system.", NATURE BIOTECHNOLOGY JAN 2000 LNKD- PUBMED:10625394, vol. 18, no. 1, January 2000 (2000-01-01), pages 66 - 70, XP002626930, ISSN: 1087-0156 * |
KIM Y-P ET AL: "Activity-based assay of matrix metalloproteinase on nonbiofouling surfaces using time-of-flight secondary ion mass spectrometry", ANALYTICAL CHEMISTRY 20080701 AMERICAN CHEMICAL SOCIETY US, vol. 80, no. 13, 1 July 2008 (2008-07-01), pages 5094 - 5102, XP002626931, DOI: DOI:10.1021/AC800299D * |
KIM Y-P ET AL: "Protein kinase assay on peptide-conjugated gold nanoparticles by using secondary-ion mass spectrometric imaging", ANGEWANDTE CHEMIE - INTERNATIONAL EDITION 2007 WILEY-VCH VERLAG DE, vol. 46, no. 36, 2007, pages 6816 - 6819, XP002626932, DOI: DOI:10.1002/ANIE.200701418 * |
Also Published As
Publication number | Publication date |
---|---|
US20110281289A1 (en) | 2011-11-17 |
WO2010006333A2 (en) | 2010-01-14 |
EP2313518A2 (en) | 2011-04-27 |
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