WO2009152482A3 - Directed differentiation and maturation of stem cell-derived cardiomyocytes - Google Patents

Directed differentiation and maturation of stem cell-derived cardiomyocytes Download PDF

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Publication number
WO2009152482A3
WO2009152482A3 PCT/US2009/047287 US2009047287W WO2009152482A3 WO 2009152482 A3 WO2009152482 A3 WO 2009152482A3 US 2009047287 W US2009047287 W US 2009047287W WO 2009152482 A3 WO2009152482 A3 WO 2009152482A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
maturation
stem cell
therapeutic
directed differentiation
Prior art date
Application number
PCT/US2009/047287
Other languages
French (fr)
Other versions
WO2009152482A2 (en
Inventor
Ronald Li
Ji-dong FU
Original Assignee
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Publication of WO2009152482A2 publication Critical patent/WO2009152482A2/en
Publication of WO2009152482A3 publication Critical patent/WO2009152482A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Abstract

This invention provides an isolated electrophysiologically immature cell or its derivative that has been modified to provide a mature electrophysiological phenotype and populations of these cells. Compositions containing these cells and populations of cells are also provided by this invention. These cells and compositions have therapeutic and diagnostic uses. Non-limiting therapeutic uses include regenerating cardiac tissue, improving cardiac function, restoring action potential of cardiac tissue; and treating or preventing cardiac malfunction. The cells and population of cells also can be used diagnostically to screen drug or other therapeutic candidate.
PCT/US2009/047287 2008-06-12 2009-06-12 Directed differentiation and maturation of stem cell-derived cardiomyocytes WO2009152482A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6109308P 2008-06-12 2008-06-12
US61/061,093 2008-06-12

Publications (2)

Publication Number Publication Date
WO2009152482A2 WO2009152482A2 (en) 2009-12-17
WO2009152482A3 true WO2009152482A3 (en) 2010-04-22

Family

ID=41417416

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/047287 WO2009152482A2 (en) 2008-06-12 2009-06-12 Directed differentiation and maturation of stem cell-derived cardiomyocytes

Country Status (1)

Country Link
WO (1) WO2009152482A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2699642A1 (en) 2007-09-12 2009-03-19 The Regents Of The University Of California Compositions and methods for improving the functional efficacy of stem cell-derived cardiomyocytes
WO2014116874A1 (en) 2013-01-23 2014-07-31 Ronald Li Engineered physical alignment of stem cell-derived cardiomyocytes
US10087436B2 (en) 2014-02-06 2018-10-02 The Regents Of The University Of California Electrophysiologically mature cardiomyocytes and methods for making same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040254134A1 (en) * 2001-04-27 2004-12-16 Eduardo Marban Biological pacemaker

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040254134A1 (en) * 2001-04-27 2004-12-16 Eduardo Marban Biological pacemaker

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SARTIANI, L. ET AL.: "Developmental Changes in Cardiomyocytes Differentiated from Human Embryonic Stem Cells: A Molecular and Electrophysiological Approach.", STEM CELLS, vol. 25, 2007, pages 1136 - 1144 *
TOMITA, Y. ET AL.: "Application of mesenchymal stem cell-derived cardiomyocytes as bio-pacemakers:current status and problems to be solved.", MED. BIO. ENG. COMPUT., vol. 45, 30 January 2007 (2007-01-30), pages 209 - 220 *
TSE, H.-F. ET AL.: "Bioartificial Sinus Node Constructed via In Vivo Gene Transfer of an Engineered Pacemaker HCN Channel Reduces the Dependence on Electronic Pacemaker in a Sick-Sinus Syndrome Model.", CIRCULATION, vol. 114, 21 August 2006 (2006-08-21), pages 1000 - 1011 *

Also Published As

Publication number Publication date
WO2009152482A2 (en) 2009-12-17

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