WO2009134327A3 - Procédés et réactifs pour augmenter la sensibilité d'une détection métallographique par enzyme - Google Patents

Procédés et réactifs pour augmenter la sensibilité d'une détection métallographique par enzyme Download PDF

Info

Publication number
WO2009134327A3
WO2009134327A3 PCT/US2009/002440 US2009002440W WO2009134327A3 WO 2009134327 A3 WO2009134327 A3 WO 2009134327A3 US 2009002440 W US2009002440 W US 2009002440W WO 2009134327 A3 WO2009134327 A3 WO 2009134327A3
Authority
WO
WIPO (PCT)
Prior art keywords
test sample
probe
reagents
sensitivity
methods
Prior art date
Application number
PCT/US2009/002440
Other languages
English (en)
Other versions
WO2009134327A2 (fr
Inventor
Richard D. Powelll
Vishwas Joshi
James F. Hainfield
Original Assignee
Powelll Richard D
JOSHI Vishwas
Hainfield James F
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Powelll Richard D, JOSHI Vishwas, Hainfield James F filed Critical Powelll Richard D
Priority to DK09739130.4T priority Critical patent/DK2269065T3/en
Priority to ES09739130.4T priority patent/ES2658949T3/es
Priority to EP09739130.4A priority patent/EP2269065B1/fr
Publication of WO2009134327A2 publication Critical patent/WO2009134327A2/fr
Publication of WO2009134327A3 publication Critical patent/WO2009134327A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Nanotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medical Informatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention porte sur un agent d’analyse qui comprend une sonde composite ayant au moins une nanoparticule ayant de multiples atomes de métal, un agent de direction et une enzyme. L'agent de direction attache la sonde à une cible dans un échantillon d’analyse. L'échantillon d’analyse et la sonde liée sont ensuite traités par un substrat d'enzyme. Un procédé de détection d'une cible dans un échantillon d’analyse comprend l'exposition de l'échantillon d’analyse à la sonde, puis le traitement de l'échantillon d’analyse par une solution d'amélioration ou de développement pour déposer au moins l'un d'un fluorophore, d'un chromogène ou d'un métal.
PCT/US2009/002440 2008-04-28 2009-04-20 Procédés et réactifs pour augmenter la sensibilité d'une détection métallographique par enzyme WO2009134327A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DK09739130.4T DK2269065T3 (en) 2008-04-28 2009-04-20 METHODS AND REAGENTS TO INCREASE THE SENSITIVITY OF ENZYM METALLOGRAPHIC DETECTION
ES09739130.4T ES2658949T3 (es) 2008-04-28 2009-04-20 Métodos y reactivos para aumentar la sensibilidad de detección metalográfica enzimática
EP09739130.4A EP2269065B1 (fr) 2008-04-28 2009-04-20 Procédés et réactifs pour augmenter la sensibilité d'une détection métallographique par enzyme

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/150,379 2008-04-28
US12/150,379 US7892781B2 (en) 2001-03-30 2008-04-28 Detecting a target using a composite probe comprising a directing agent, a metal nanoparticle and an enzyme

Publications (2)

Publication Number Publication Date
WO2009134327A2 WO2009134327A2 (fr) 2009-11-05
WO2009134327A3 true WO2009134327A3 (fr) 2009-12-30

Family

ID=41255605

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/002440 WO2009134327A2 (fr) 2008-04-28 2009-04-20 Procédés et réactifs pour augmenter la sensibilité d'une détection métallographique par enzyme

Country Status (5)

Country Link
US (1) US7892781B2 (fr)
EP (1) EP2269065B1 (fr)
DK (1) DK2269065T3 (fr)
ES (1) ES2658949T3 (fr)
WO (1) WO2009134327A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201003594D0 (en) * 2010-03-04 2010-04-21 Eluceda Ltd Method for detecting the presence of specific micro-organisms and device for the same
WO2011139792A2 (fr) 2010-04-27 2011-11-10 Ventana Medical Systems, Inc. Conjugués anticorps-nanoparticules et procédés de fabrication et d'utilisation de tels conjugués
KR101208659B1 (ko) 2011-08-30 2012-12-05 한국기계연구원 색상 변화 특성을 이용한 검출방법
WO2013148498A1 (fr) 2012-03-27 2013-10-03 Ventana Medical Systems, Inc. Conjugués de signalisation, et procédés d'utilisation
JP6720138B2 (ja) 2015-03-13 2020-07-08 シスメックス株式会社 被検物質の検出方法およびその方法に用いられる試薬キット
IT201700087291A1 (it) * 2017-07-28 2019-01-28 Fondazione St Italiano Tecnologia Metodo di imaging di un campione biologico e relativa sonda
CN110864940A (zh) * 2018-08-28 2020-03-06 国家纳米科学中心 一种透射电镜的原位光-电显微镜关联检测的样品预处理方法及应用
CN113340696B (zh) * 2021-07-20 2023-05-12 中国航发成都发动机有限公司 一种热喷涂涂层有机封孔漆的金相检测方法
CN117848974B (zh) * 2024-03-07 2024-05-28 陕西长空齿轮有限责任公司 一种合金表面氧化金相检测方法及系统

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040253624A1 (en) * 2002-11-26 2004-12-16 Smith Roger E. Microporous materials, methods of making, using, and articles thereof
US20050100976A1 (en) * 2003-06-24 2005-05-12 Christopher Bieniarz Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US74457A (en) 1868-02-11 Arthur wadsworth
US5116734A (en) * 1989-09-01 1992-05-26 Digene Diagnostics, Inc. Highly sensitive method for detecting peroxidase
US6121425A (en) * 1994-07-29 2000-09-19 Nanoprobes, Inc. Metal-lipid molecules
US5595878A (en) * 1995-06-02 1997-01-21 Boron Biologicals, Inc. Detection of biopolymers and biooligomers with boron hydride labels
US6358682B1 (en) * 1998-01-26 2002-03-19 Ventana Medical Systems, Inc. Method and kit for the prognostication of breast cancer
US6911306B1 (en) * 1999-10-18 2005-06-28 Emory University TMS1 compositions and methods of use
US6534039B2 (en) * 2000-07-21 2003-03-18 James F. Hainfeld Extended organic cobalt and nickel magnetic complexes
US6670113B2 (en) * 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
US7364872B1 (en) * 2001-03-30 2008-04-29 Nanoprobes Test methods using enzymatic deposition and alteration of metals
JP2005520795A (ja) * 2001-12-12 2005-07-14 コンフォーマ・セラピューティクス・コーポレイション Ｈｓｐ９０阻害活性を有するプリン類似体

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040253624A1 (en) * 2002-11-26 2004-12-16 Smith Roger E. Microporous materials, methods of making, using, and articles thereof
US20050100976A1 (en) * 2003-06-24 2005-05-12 Christopher Bieniarz Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest

Also Published As

Publication number Publication date
US20080318249A1 (en) 2008-12-25
EP2269065A2 (fr) 2011-01-05
DK2269065T3 (en) 2018-01-02
EP2269065B1 (fr) 2017-11-22
US7892781B2 (en) 2011-02-22
WO2009134327A2 (fr) 2009-11-05
ES2658949T3 (es) 2018-03-13
EP2269065A4 (fr) 2012-08-22

Similar Documents

Publication Publication Date Title
WO2009134327A3 (fr) Procédés et réactifs pour augmenter la sensibilité d'une détection métallographique par enzyme
EP1996940A4 (fr) Détection d'un cancer par dépistage de taux élevés de bcl-2
WO2010036827A8 (fr) Procédé de détection d'analytes
WO2007067680A3 (fr) Caractérisation d'analyse à base particulaire
ATE556030T1 (de) Ultrasensitiver nachweis von analyten
DE602005013225D1 (de) Aptamer-nanopartikelkonjugate und verwendungsverfahren zur detektion von zielanalyten
NZ596163A (en) Diagnostic devices and related methods
WO2011109364A3 (fr) Détection ultrasensible de molécules ou de particules à l'aide de billes ou autres objets de capture
MY157955A (en) Detection of a target antigen irrespective of the presence or absence of a corresponding therapeutic antibody
MX341517B (es) Biomarcadores de cancer pancreatico y usos de los mismos.
WO2006020579A3 (fr) Procede d'identification rapide de micro-organismes
WO2007098279A3 (fr) Détection d'analytes au moyen de polymères à code-barres
WO2006069023A3 (fr) Procede et appareil d'analyse avec effets de matrices d'echantillons reduits
WO2007005666A3 (fr) Systeme et methode de detection d'analytes au moyen de recepteurs differentiels
WO2008060651A3 (fr) Sparc et procédés d'utilisation de celui-ci
ATE514086T1 (de) Nachweis von zielmolekülen in einer probe unter verwendung eines magnetfelds
WO2008028031A3 (fr) Marqueurs et méthodes d'évaluation et de traitement de recocolite hémorragique et de troubles associées à l'aide d'un panel de 43 gènes
WO2006007403A3 (fr) Methodes d'identification et de mesure de liaisons s-nitrosothiol dans des cellules et des molecules contenant des hemes
ATE533855T1 (de) Testverfahren zum nachweis lebensfähiger mikrobenzellen in einer probe
ATE408829T1 (de) Verfahren und assaykits zum nachweis mononuklearer zellphänotypen
WO2009123996A3 (fr) Procédé de quantification d’hybridation pour des oligonucléotides à base de micro-arn et micro-adn modifiés
WO2010051544A3 (fr) Plateforme de diagnostic rapide homogène basée sur le transfert d'énergie par résonance de fluorescence des lanthanides
WO2008070333A3 (fr) Détection automatisée du cancer et hyperplasies de grade élevé
WO2010006201A3 (fr) Procédés de réduction d'une interaction non spécifique dans des essais de nanoparticules métalliques
MX2009010751A (es) Composiciones y metodos de deteccion.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09739130

Country of ref document: EP

Kind code of ref document: A2

REEP Request for entry into the european phase

Ref document number: 2009739130

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2009739130

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE