WO2009127686A1 - Novel estrogen receptor ligands - Google Patents

Novel estrogen receptor ligands Download PDF

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Publication number
WO2009127686A1
WO2009127686A1 PCT/EP2009/054521 EP2009054521W WO2009127686A1 WO 2009127686 A1 WO2009127686 A1 WO 2009127686A1 EP 2009054521 W EP2009054521 W EP 2009054521W WO 2009127686 A1 WO2009127686 A1 WO 2009127686A1
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WO
WIPO (PCT)
Prior art keywords
indole
phenyl
hydroxy
carbonitrile
alkyl
Prior art date
Application number
PCT/EP2009/054521
Other languages
French (fr)
Inventor
Patrik RHÖNNSTAD
Elisabet Kallin
Theresa Apelqvist
Mattias WENNERSTÅL
Aiping Cheng
Original Assignee
Karo Bio Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0806944A external-priority patent/GB0806944D0/en
Priority claimed from GB0823367A external-priority patent/GB0823367D0/en
Priority to MX2010011408A priority Critical patent/MX2010011408A/en
Priority to RS20150525A priority patent/RS54165B1/en
Application filed by Karo Bio Ab filed Critical Karo Bio Ab
Priority to AU2009237657A priority patent/AU2009237657B2/en
Priority to JP2011504464A priority patent/JP5635493B2/en
Priority to NZ588121A priority patent/NZ588121A/en
Priority to BRPI0910656A priority patent/BRPI0910656A2/en
Priority to CA2720215A priority patent/CA2720215C/en
Priority to US12/736,438 priority patent/US8367665B2/en
Priority to DK09731606.1T priority patent/DK2276732T3/en
Priority to CN200980122752.2A priority patent/CN102066323B/en
Priority to KR1020167014182A priority patent/KR20160067981A/en
Priority to PL09731606T priority patent/PL2276732T3/en
Priority to EP20090731606 priority patent/EP2276732B1/en
Priority to SI200931250T priority patent/SI2276732T1/en
Priority to RU2010146485/04A priority patent/RU2492164C2/en
Priority to ES09731606.1T priority patent/ES2543599T3/en
Publication of WO2009127686A1 publication Critical patent/WO2009127686A1/en
Priority to IL208392A priority patent/IL208392A/en
Priority to ZA2010/07255A priority patent/ZA201007255B/en
Priority to HK11106180.4A priority patent/HK1152039A1/en
Priority to US13/739,124 priority patent/US8653072B2/en
Priority to HRP20150864TT priority patent/HRP20150864T1/en

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Definitions

  • This invention relates to compounds which are estrogen receptor ligands and are preferably selective for the estrogen receptor ⁇ isoform, to methods of preparing such compounds and to methods for using such compounds in treatment of diseases related to the estrogen receptor such as depressive disorders, anxiety disorders, Alzheimer's disease, cognitive disorders, osteoporosis, elevated blood triglyceride levels, atherosclerosis, endometriosis, urinary incontinence, autoimmune disease, and cancer of the lung, colon, breast, uterus and prostate.
  • diseases related to the estrogen receptor such as depressive disorders, anxiety disorders, Alzheimer's disease, cognitive disorders, osteoporosis, elevated blood triglyceride levels, atherosclerosis, endometriosis, urinary incontinence, autoimmune disease, and cancer of the lung, colon, breast, uterus and prostate.
  • the estrogen receptor is a ligand activated mammalian transcription factor involved in the up and down regulation of gene expression.
  • the natural hormone for the estrogen receptor is ⁇ -17-estradiol (E2) and closely related metabolites. Binding of estradiol to the estrogen receptor causes a dimerization of the receptor and the dimer in turn binds to estrogen response elements (ERE' s) on DNA.
  • E2 ⁇ -17-estradiol
  • E2 estrogen response elements
  • the ER/DNA complex recruits other transcription factors responsible for the transcription of DNA downstream from the ERE into mRNA which is eventually translated into protein.
  • the interaction of ER with DNA may be indirect through the intermediacy of other transcription factors, most notably fos and jun.
  • Estrogens are critical for sexual development in females.
  • estrogens play an important role in maintaining bone density, regulation of blood lipid levels, and appear to have neuroprotective effects. Consequently decreased estrogen production in post-menopausal women is associated with a number of diseases such as osteoporosis, atherosclerosis, depression and cognitive disorders.
  • certain types of proliferative diseases such as breast and uterine cancer and endometriosis are stimulated by estrogens and therefore antiestrogens (i.e., estrogen antagonists) have utility in the prevention and treatment of these types of disorders.
  • the pleiotropic nature of natural estrogen precludes its widespread, more chronic use due to the increased risk of proliferative effects on breast, uterine and ovarian tissues.
  • the identification of the estrogen receptor, ER ⁇ has provided a means by which to identify more selective estrogen agents which have the desired anti-depressant activity in the absence of the proliferative effects which are mediated by ERa.
  • therapeutic agents having ER ⁇ -selectivity are potentially effective in the treatment of depression.
  • WO 2006/019831 discloses certain indole derivatives having utility in the prevention or treatment of Hepatitis C viral infection.
  • WO 2005/018636 discloses certain indole derivative having estrogen receptor modulator activity, all said indoles being oximes.
  • the compounds of the present invention are ligands for estrogen receptors and as such may be useful for treatment or prevention of a variety of conditions related to estrogen functioning including bone loss, bone fractures, osteoporosis, cartilage degeneration, endometriosis, uterine fibroid disease, hot flashes, increased levels of LDL cholesterol, cardiovascular disease, impairment of cognitive functioning, age- related mild cognitive impairment, cerebral degenerative disorders, restenosis, gynecomastia, vascular smooth muscle cell proliferation, obesity, incontinence, anxiety, depression, perimenopausal depression, post-partum depression, premenstrual syndrome, manic depression, dementia, obsessive compulsive behavior, attention deficit disorder, attention deficit hyperactivity disorder, sleep disorders, irritability, impulsivity, anger management, hearing disorders, multiple sclerosis, Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, spinal cord injury, stroke, autoimmune disease, inflammation, IBD, IBS, sexual dysfunction, hyper
  • This invention provides a compound of formula (I) or a pharmaceutically acceptable ester, amide, carbamate, solvate or salt thereof, including a salt of such an ester, amide or carbamate, and a solvate of such an ester, amide, carbamate or salt,
  • R 1 is selected from the group consisting of halogen, cyano, nitro, OR ⁇ , N(R B ) 2 , -C(O)Ci_ 4 alkyl, -SO 2 C M alkyl, C 1-6 alkyl, C 2-6 alkenyl, C 2 - 6 alkynyl, haloCi_ 6 alkyl, dihaloCi -6 alkyl, trihaloCi_ 6 alkyl, haloC 2 _ 6 alkenyl, dihaloC 2 _ 6 alkenyl, trihaloC 2 - 6 alkenyl, cyanoCi_ 6 alkyl, Ci_ 4 alkoxyCi.
  • Ci_ 6 alkyl C 2 - 6 alkenyl, C 2 . 6 alkynyl, haloCi_ 6 alkyl, dihaloC ⁇ alkyl and trihaloC ⁇ alkyl; provided that if one of R 1 and R 2 represents halogen, the other must represent a group other than halogen;
  • each of R 3 , R 4 , R 5 and R 6 is independently selected from the group consisting of hydrogen, 0R A , halogen, cyano, nitro, Ci. 6 alkyl, C 2 . 6 alkenyl, C 2 . 6 alkynyl, haloCi_ 6 alkyl, dihaloCi_ 6 alkyl and trihaloC ⁇ alkyl; each R A is independently selected from the group consisting of hydrogen, Ci_ 6 alkyl, C 2 _ 6 alkenyl, C 2 .
  • each R B is independently selected from the group consisting of hydrogen, Ci_ 6 alkyl, C 2 _ 6 alkenyl, C 2 . 6 alkynyl, Cj_ 8 cycloalkyl, C 3 _ g cycloalkylCi_ 6 alkyl, C ⁇ -ioaryl and C 640 arylCi_ 6 alkyl, each optionally substituted by from 1 to 3 halogen atoms;
  • the compound of formula (I) is not 4-[3-(4,5-Dihydro-lH-imidazol-2-yl)-2-(3,5-dimethyl-isoxazol-4-yl)-indol-l -yl]-phenol; l-(4-Hydroxy-phenyl)-2-(4-methyl-imidazol-l-yl)-lH-indole-3-carbonitrile;
  • Compounds of the invention have surprisingly been found to be ligands of the estrogen receptor.
  • the compounds accordingly have use in the treatment or prophylaxis of conditions associated with estrogen receptor activity.
  • the compounds of the invention may contain chiral (asymmetric) centers or the molecule as a whole may be chiral.
  • the individual stereoisomers (enantiomers and diastereoisomers) and mixtures of these are within the scope of the present invention.
  • the present invention provides compounds that are estrogen receptor ligands.
  • Estrogen receptor ligand as used herein is intended to cover any moiety which binds to an estrogen receptor.
  • the ligand may act as an agonist, a partial agonist, an antagonist or a partial antagonist.
  • the ligand may be ER ⁇ selective or display mixed ERa and ER ⁇ activity.
  • the ligand may act both as an agonist or a partial agonist of ER ⁇ and as an antagonist or a partial antagonist of ERa.
  • R 1 represents a heterocyclyl group
  • this group may be saturated or unsaturated, and may contain one or more O, N and/or S atoms. It is preferably 5- or 6-membered.
  • Suitable heterocyclyl groups include furyl, thienyl, pyrrolyl, pyrrolinyl, pyrrolidinyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, imidazolyl, imidazolinyl, imidazolidine, pyrazoly, pyrazolinyl, pyrazolidinyl, pyridyl, morpholinyl, and piperidyl, with isoxazolyl being a particularly preferred heterocyclyl group.
  • Preferred substituents for a heterocyclyl group include 1 to 3, for example 1 or 2, substituents, each substituent being selected from the group consisting of OR A , halogen, cyano, -C(O)C]. 4 alkyl, C M alkyl, C 2 - 4 alkenyl, C 2 . 4 alkynyl, haloCj. 4 alkyl, dihaloCi_ 4 alkyl and Especially preferred substituents are selected from halogen, cyano, Ci_ 4 alkyl (especially methyl), -C(O)Ci_ 4 alkyl, and OR A in which R A preferably represents a hydrogen atom or a group. More especially preferred substituents are selected from halogen, cyano and C M alkyl (especially methyl or ethyl).
  • Preferred substituents for a phenyl or benzyl group R include those mentioned above for a heterocyclyl group R 1 .
  • R 2 represents a heterocyclyl group
  • this group may for example be one of the preferred groups mentioned above for R 1 .
  • Preferred substituents for a phenyl or benzyl group R 2 include those mentioned above for a heterocyclyl group R 1 .
  • each R A is preferably independently selected from the group consisting of hydrogen, Ci_ 4 alkyl, C 2 - 4 alkenyl, C 2 _ 4 alkynyl, C 3 _ 6 cycloalkyl, phenyl and benzyl.
  • each R A independently represents hydrogen or Ci_ 4 alkyl, especially methyl.
  • each R B is preferably independently selected from the group consisting of hydrogen and Ci_ 4 alkyl.
  • R 1 is selected from the group consisting of OR A , N(R B ) 2 , -C(O)C ,. 4 alkyl, C,. 6 alkyl, C 2 . 6alkenyl, C 2 . 6 alkynyl, haloC M alkyl, dihaloCi_ 4 alkyl, trihaloCi_ 4 alkyl, haloC 2 . 4 alkenyl, dihaloC 2 . 4 alkenyl, trihaloC 2 _ 4 alkenyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted as above.
  • R 1 is selected from the group consisting of OR A , N(R B ) 2 , -C(O)C M alkyl, C,. 4 alkyl, C 2 . 4 alkenyl, C 2 . 4 alkynyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted as above.
  • R 2 may for example be selected from one of the preferred groups mentioned above for R 1 .
  • scycloalkyl C 3 -scycloalkylCi_ 6 alkyl, phenyl, benzyl and 5-10 membered heterocyclyl wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents each substituent being selected from the group consisting of OR A , halogen, cyano, nitro, Ci_ 6 alkyl, C 2 _ 6 alkenyl, C 2 _ 6 alkynyl, haloCi_ 6 alkyl,
  • each of R 3 , R 4 , R 5 and R 6 is selected from the group consisting of hydrogen, 0R ⁇ , halogen, cyano, Ci. 4 alkyl, for example methyl, haloCi_ 4 alkyl, for example chloro- or fiuoro-methyl, dihaloCi. 4 alkyl, for example di chloro- or difluoromethyl, and trihaloCi_ 4 alkyl, for example trichloro- or trifluoromethyl.
  • each of R 3 , R 4 , R 5 and R 6 is selected from the group consisting of hydrogen, OH, halogen, cyano, methyl, or trifluoromethyl. Most preferably at least one of R 3 and R 4 is hydrogen.
  • each of R 5 and R 6 independently represents hydrogen and/or halogen, especially fluorine.
  • R 1 is selected from the group consisting of OR A , N(R B ) 2 , -C(O)C M alkyl, C I-6 alkyl, C 2 . 6 alkenyl, C 2 . 6 alkynyl, haloCi -4 alkyl, dihaloQ.
  • R 1 is selected from the group consisting of OR A , N(R B ) 2 , -C(O)Ci_ 4 alkyl, Ci ⁇ alkyl, C 2-4 alkenyl, C 2 _ 4 alkynyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted as above;
  • each of R 3 , R 4 , R 5 and R 6 is selected from the group consisting of hydrogen, 0R A , halogen, cyano, Ci_ 4 alkyl, haloCi_ 4 alkyl, dihaloCi. 4 alkyl, and trihaloCi_ 4 alkyl, especially hydrogen, OH, halogen, cyano, methyl, or trifluoromethyl; especially, each of R 5 and R 6 represents hydrogen and/or halogen, especially fluorine;
  • each R A is preferably independently selected from the group consisting of hydrogen, C 2 _ 4 alkenyl, C 2 . 4 alkynyl, C 3 _ 6 cycloalkyl, phenyl and benzyl, especially hydrogen and Ci_ 4 alkyl, especially methyl; and
  • each R B is independently selected from the group consisting of hydrogen and C,_ 4 alkyl.
  • this invention provides a compound of formula (I) or a pharmaceutically acceptable ester, amide, solvate or salt thereof, including a salt of such an ester or amide, and a solvate of such an ester, amide or salt,
  • R 1 is selected from the group consisting of halogen, cyano, nitro, 0R A , N(R B ) 2 , -C(O)Ci_ 4 alkyl, -SO 2 C
  • each of R 3 , R 4 , R 5 and R 6 is independently selected from the group consisting of hydrogen, 0R A , halogen, cyano, nitro, Ci_ 6 alkyl, C 2 . 6 alkenyl, C 2 _ 6 alkynyl, haloCi_ 6 alkyl, dihaloCi_ 6 alkyl and trihaloCi_ 6 alkyl;
  • each R ⁇ is independently selected from the group consisting of hydrogen, C]_ 6 alkyl, C 2 . 6 alkenyl, C 2 . 6alkynyl, C 3 _ 8 cycloalkyl, C 3 _ 8 cycloalkylCi_ 6 alkyl, C ⁇ -ioaryl and C 6 _io arylCi_ 6 alkyl, each optionally substituted by from 1 to 3 halogen atoms; and
  • each R B is independently selected from the group consisting of hydrogen, Ci_ 6 alkyl, C 2 . 6 alkenyl, C 2 . ⁇ alkynyl, C 3 _ 8 cycloalkyl, Cv 8 cycloalkylC]_ 6 alkyl, C ⁇ -ioaryl and C 6 -io arylCi_ 6 alkyl, each optionally substituted by from 1 to 3 halogen atoms;
  • the compound of formula (I) is not 4-[3-(4,5-Dihydro-lH-imidazol-2-yl)-2-(3,5- dimethyl-isoxazol-4-yl)-indol-l -yl]-phenol.
  • Compounds of the formula I include, but are not limited to, the compounds specifically named in the Examples herein.
  • the compound names were generated in accordance with IUPAC by the ACD Labs 8.0/name program, version 8.05 and/or with ISIS DRAW Autonom 2000 and/or ChemBioDraw Ultra version 11.0.
  • the compounds may form esters, amides, carbamates and/or salts. Salts and solvates of compounds of formula (I) which are suitable for use in medicine are those wherein a counterion or associated solvent is pharmaceutically acceptable.
  • salts and solvates having non-pharmaceutically acceptable counterions or associated solvents are within the scope of the present invention, for example, for use as intermediates in the preparation of the compounds of formula (I) and their pharmaceutically acceptable salts, solvates and physiologically functional derivatives.
  • physiologically functional derivative is meant a chemical derivative of a compound of formula (I) having the same physiological function as the free compound of formula (I), for example, by being convertible in the body thereto.
  • Esters, amides and carbamates are examples of physiologically functional derivatives.
  • Suitable salts according to the invention include those formed with organic or inorganic acids or bases, hi particular, suitable salts formed with acids according to the invention include those formed with mineral acids, strong organic carboxylic acids, such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted, for example, by halogen, such as saturated or unsaturated dicarboxylic acids, such as hydroxycarboxylic acids, such as amino acids, or with organic sulfonic acids, such as (C 1 - C 4 )-alkyl- or aryl-sulfonic acids which are unsubstituted or substituted, for example by halogen.
  • mineral acids such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted, for example, by halogen, such as saturated or unsaturated dicarboxylic acids, such as hydroxycarboxylic acids, such as amino acids, or with organic sulfonic acids, such as (
  • Pharmaceutically acceptable acid addition salts include those formed from hydrochloric, hydrobromic, sulphuric, nitric, citric, tartaric, acetic, phosphoric, lactic, pyruvic, acetic, trifluoroacetic, succinic, perchloric, fumaric, maleic, glycolic, lactic, salicylic, oxaloacetic, methanesulfonic, ethanesulfonic, p- toluenesulfonic, formic, benzoic, malonic, naphthalene-2-sulfonic, benzenesulfonic, isethionic, ascorbic, malic, phthalic, aspartic, and glutamic acids, lysine and arginine.
  • Other acids such as oxalic, while not in themselves pharmaceutically acceptable, may be useful as intermediates in obtaining the compounds of the invention and their pharmaceutical acceptable acid addition salts.
  • Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts, for example those of potassium and sodium, alkaline earth metal salts, for example those of calcium and magnesium, and salts with organic bases, for example dicyclohexylamine, N-methyl-D-glucomine, morpholine, thiomorpholine, piperidine, pyrrolidine, a mono-, di- or tri-lower alkylamine, for example ethyl-, tert-butyl-, diethyl-, diisopropyl-, triethyl-, tributyl- or dimethyl -propylamine, or a mono-, di- or trihydroxy lower alkylamine, for example mono-, di- or triethanolamine. Corresponding internal salts may furthermore be formed.
  • Compounds of formula (I) may have an appropriate group converted to an ester, an amide or a carbamate.
  • typical ester and amide groups formed from an acid group in the compound of the formula I include -COOR B , -CONR B 2 , -SO 2 . OR B , or -SO 2 .NR B 2
  • typical ester and amide and carbamate groups formed from an -OH or -NHR B group in the compound of the formula I include -O.CO.R B , -NR B .CO.R B , -NR B . CO 2 R 8 -O.SO 2 R B , and -NR B SO 2 R B , where R B has one of the meanings given above.
  • a compound which, upon administration to the recipient, is capable of being converted into a compound of formula (I) as described above, or an active metabolite or residue thereof, is known as a "prodrug".
  • a prodrug may, for example, be converted within the body, e. g. by hydrolysis in the blood, into its active form that has medical effects.
  • Pharmaceutical acceptable prodrugs are described in T. Higuchi and V. Stella, Prodrugs as Novel Delivery Systems, Vol. 14 of the A. C. S. Symposium Series (1976); "Design of Prodrugs” ed. H. Bundgaard, Elsevier, 1985; and in Edward B. Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987, which are incorporated herein by reference.
  • alkyl means both straight and branched chain saturated hydrocarbon groups.
  • alkyl groups include methyl, ethyl, n-propyl, iso-propyl, n-butyl, t-butyl, i-butyl, sec-butyl, pentyl and hexyl groups.
  • unbranched alkyl groups there are preferred methyl, ethyl, n-propyl, iso-propyl, n-butyl groups.
  • branched alkyl groups there may be mentioned t-butyl, i-butyl, 1- ethylpropyl and 1 -ethylbutyl groups.
  • alkoxy means the group O-alkyl, where "alkyl” is used as described above.
  • alkoxy groups include methoxy and ethoxy groups. Other examples include propoxy and butoxy.
  • alkenyl means both straight and branched chain unsaturated hydrocarbon groups with at least one carbon carbon double bond.
  • alkenyl groups include ethenyl, propenyl, butenyl, pentenyl and hexenyl.
  • Preferred alkenyl groups include ethenyl, 1- propenyl and 2- propenyl.
  • alkynyl means both straight and branched chain unsaturated hydrocarbon groups with at least one carbon carbon triple bond.
  • alkynyl groups include ethynyl, propynyl, butynyl, pentynyl and hexynyl.
  • Preferred alkynyl groups include ethynyl 1- propynyl and 2- propynyl.
  • cycloalkyl means a saturated group in a ring system.
  • a cycloalkyl group can be monocyclic or bicyclic.
  • a bicyclic group may, for example, be fused or bridged.
  • monocyclic cycloalkyl groups include cyclopropyl, cyclobutyl and cyclopentyl.
  • Other examples of monocyclic cycloalkyl groups are cyclohexyl, cycloheptyl and cyclooctyl.
  • Examples of bicyclic cycloalkyl groups include bicyclo [2. 2.1]hept-2-yl.
  • the cycloalkyl group is monocyclic.
  • aryl means a monocyclic or bicyclic aromatic carbocyclic group.
  • aryl groups include phenyl and naphthyl. A naphthyl group may be attached through the 1 or the 2 position.
  • one of the rings may, for example, be partially saturated. Examples of such groups include indanyl and tetrahydronaphthyl.
  • C 5-I0 aryl is used herein to mean a group comprising from 5 to 10 carbon atoms in a monocyclic or bicyclic aromatic group.
  • a particularly preferred C 5-I0 aryl group is phenyl.
  • halogen means fluorine, chlorine, bromine or iodine. Fluorine, chlorine and bromine are particularly preferred.
  • haloalkyl means an alkyl group having a halogen substituent
  • dihaloalkyl means an alkyl group having two halogen substituents
  • trihaloalkyl means an alkyl group having three halogen substituents.
  • haloalkyl groups include fluoromethyl, chloromethyl, bromomethyl, fluoromethyl, fluoropropyl and fluorobutyl groups; examples of dihaloalkyl groups include difluoromethyl and difluoroethyl groups; examples of triihaloalkyl groups include trifluoromethyl and trifluoroethyl groups.
  • heterocyclyl means an aromatic or a non-aromatic cyclic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen or sulfur.
  • a heterocyclyl group may, for example, be monocyclic or bicyclic. In a bicyclic heterocyclyl group there may be one or more heteroatoms in each ring, or only in one of the rings.
  • a heteroatom is preferably O or N.
  • Heterocyclyl groups containing a suitable nitrogen atom include the corresponding N-oxides.
  • monocyclic non-aromatic heterocyclyl groups include aziridinyl, azetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, piperidinyl, piperazinyl, tetrahydrofuranyl, telrahydropyranyl, morpholinyl, thiomorpholinyl and azepanyl.
  • bicyclic heterocyclyl groups in which one of the rings is non-aromatic include dihydrobenzofuranyl, indanyl, indolinyl, isoindolinyl, tetrahydroisoquinolinyl, tetrahydroquinolyl and ben ⁇ oazepanyl.
  • monocyclic aromatic heterocyclyl groups include furanyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, oxadiazolyl, thiadiazolyl, pyridyl, triazolyl, triazinyl, pyridazyl, isothiazolyl, isoxazolyl, pyrazinyl, pyrazolyl and pyrimidinyl.
  • bicyclic aromatic heterocyclyl groups include quinoxalinyl, quinazolinyl, pyridopyrazinyl, benzoxazolyl, benzothiophenyl, benzimidazolyl, naphthyridinyl, quinolinyl, benzofuranyl, indolyl, benzothiazolyl, oxazolyl[4,5-b]pyridiyl, pyridopyrimidinyl, isoquinolinyl and benzodroxazole.
  • heterocyclyl groups examples include piperidinyl, tetrahydrofuranyl, tetrahydropyranyl, pyridyl, pyrimidinyl and indolyl.
  • Preferred heterocyclyl groups also include thienyl, thiazolyl, furanyl, pyrazolyl, pyrrolyl, isoxazolyl and imidazolyl.
  • cycloalkylalkyl means a group cycloalkyl-alkyl- attached through the alkyl group, "cycloalkyl” and “alkyl” being understood to have the meanings outlined above.
  • the compounds of the invention have activity as estrogen receptor ligands.
  • the compounds of the invention have activity as estrogen receptor modulators, and may be agonists, partial agonists, antagonists, or partial antagonists of the estrogen receptor.
  • Particularly preferred compounds of the invention have activity as an agonist or a partial agonist of ER ⁇ .
  • Preferred compounds of this type are selective agonists of the estrogen receptor-beta (ER ⁇ ).
  • the compounds of the invention may thus be used in the treatment of diseases or disorders associated with estrogen receptor activity.
  • the compounds of the invention that are agonists or partial agonists of the estrogen receptor may be used in the treatment of diseases or disorders for which selective agonists or partial agonists of the estrogen receptor are indicated.
  • the compounds of the invention that are antagonists or partial antagonists of the estrogen receptor may be used in the treatment of diseases or disorders for which selective antagonists or partial antagonists of the estrogen receptor are indicated.
  • Clinical conditions for which an agonist or partial agonist is indicated include, but are not limited to, bone loss, bone fractures, osteoporosis, cartilage degeneration, endometriosis, uterine fibroid disease, hot flashes, increased levels of LDL cholesterol, cardiovascular disease, impairment of cognitive functioning, cerebral degenerative disorders, restenosis, gynecomastia, vascular smooth muscle cell proliferation, obesity, incontinence, anxiety, depression, autoimmune disease, inflammation, IBD, IBS, sexual dysfunction, hypertension, retinal degeneration, and lung, colon, breast, uterus, and prostate cancer, and/or disorders related to estrogen functioning.
  • the compounds of the invention find particular application in the treatment or prophylaxis of the following: bone loss, bone fractures, osteoporosis, cartilage degeneration, endometriosis, uterine fibroid disease, hot flashes, increased levels of LDL cholesterol, cardiovascular disease, impairment of cognitive functioning, age-related mild cognitive impairment, cerebral degenerative disorders, restenosis, gynecomastia, vascular smooth muscle cell proliferation, obesity, incontinence, anxiety, depression, perimenopausal depression, post-partum depression, premenstrual syndrome, manic depression, dementia, obsessive compulsive behavior, attention deficit disorder, attention deficit hyperactivity disorder, sleep disorders, irritability, impulsivity, anger management, hearing disorders, multiple sclerosis, Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, spinal cord injury, stroke, autoimmune disease, inflammation, IBD, IBS, sexual dysfunction, hypertension, retinal degeneration, lung cancer, colon cancer, breast cancer
  • the present compounds finds particular application in the treatment or prophylaxis of depression, perimenopausal depression, post-partum depression, premenstrual syndrome and manic depression.
  • the treatment or prophylaxis of hot flashes (or hot flushes) in males is preferable for patients that has had an androgen ablation for treatment of prostate cancer.
  • depression includes but is not limited to, major depressive disorder, dysthymic disorder, bipolar disorder, cyclothymic disorder, mood disorder due to a general medical condition, substance- induced mood misorder, seasonal affective disorder (SAD), postpartum depression and premenstrual dysphoric disorder.
  • the invention also provides a method for the treatment or prophylaxis of a condition in a mammal mediated by an estrogen receptor, which comprises administering to the mammal a therapeutically effective amount of a compound according to the invention.
  • Clinical conditions mediated by an estrogen receptor that may be treated by the method of the invention are preferably those described above.
  • the invention also provides the use of a compound according to the invention, for the manufacture of a medicament for the treatment or prophylaxis of a condition mediated by an estrogen receptor.
  • Clinical conditions mediated by an estrogen receptor that may be treated by the method of the invention are preferably those described above.
  • the amount of active ingredient which is required to achieve a therapeutic effect will, of course, vary with the particular compound, the route of administration, the subject under treatment, including the type, species, age, weight, sex, and medical condition of the subject and the renal and hepatic function of the subject, and the particular disorder or disease being treated, as well as its severity.
  • An ordinarily skilled physician, veterinarian or clinician can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.
  • Oral dosages of the present invention when used for the indicated effects, will range between about 0.01 mg per kg of body weight per day (mg/kg/day) to about 100 mg/kg/day, preferably 0.01 mg per kg of body weight per day (mg/kg/day) to 10 mg/kg/day, and most preferably 0.1 to 5.0 mg/kg/day, for adult humans.
  • the compositions are preferably provided in the form of tablets or other forms of presentation provided in discrete units containing 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 5.0, 10.0, 15.0, 25.0, 50.0, 100, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated.
  • a medicament typically contains from about 0.01 mg to about 500 mg of the active ingredient, preferably from about 1 mg to about 100 mg of active ingredient.
  • the most preferred doses will range from about 0.1 to about 10 mg/kg/minute during a constant rate infusion.
  • compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily.
  • preferred compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using those forms of transdermal skin patches well known to those of ordinary skill in the art.
  • the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen.
  • the invention provides a pharmaceutical formulation comprising a compound according to the invention, and a pharmaceutically acceptable diluent, excipient or carrier (collectively referred to herein as "carrier" materials).
  • carrier a pharmaceutically acceptable diluent, excipient or carrier
  • Pharmaceutical compositions of the invention may take the form of a pharmaceutical formulation as described below.
  • the pharmaceutical formulations according to the invention include those suitable for oral, parenteral (including subcutaneous, intradermal, intramuscular, intravenous [bolus or infusion], and intraarticular), inhalation (including fine particle dusts or mists which may be generated by means of various types of metered does pressurized aerosols), nebulizers or insufflators, rectal, intraperitoneal and topical (including dermal, buccal, sublingual, and intraocular) administration, although the most suitable route may depend upon, for example, the condition and disorder of the recipient.
  • parenteral including subcutaneous, intradermal, intramuscular, intravenous [bolus or infusion], and intraarticular
  • inhalation including fine particle dusts or mists which may be generated by means of various types of metered does pressurized aerosols
  • nebulizers or insufflators rectal, intraperitoneal and topical (including dermal, buccal, sublingual, and intraocular) administration, although the most suitable route may depend upon,
  • the formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing the active ingredient into association with the carrier which constitutes one or more accessory ingredients. In general the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.
  • Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, pills or tablets each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid, for example as elixirs, tinctures, suspensions or syrups; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion.
  • the active ingredient may also be presented as a bolus, electuary or paste.
  • a tablet may be made by compression or moulding, optionally with one or more accessory ingredients.
  • Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free- flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, lubricating, surface active or dispersing agent.
  • Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.
  • the tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein.
  • the present compounds can, for example, be administered in a form suitable for immediate release or extended release.
  • Immediate release or extended release can be achieved by the use of suitable pharmaceutical compositions comprising the present compounds, or, particularly in the case of extended release, by the use of devices such as subcutaneous implants or osmotic pumps.
  • the present compounds can also be administered liposomally.
  • compositions for oral administration include suspensions which can contain, for example, microcrystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer, and sweeteners or flavoring agents such as those known in the art; and immediate release tablets which can contain, for example, microcrystalline cellulose, dicalcium phosphate, starch, magnesium stearate, calcium sulfate, sorbitol, glucose and/or lactose and/or other excipients, binders, extenders, disintegrants, diluents and lubricants such as those known in the art.
  • Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes and the like.
  • Disintegrators include without limitation starch, methylcellulose, agar, bentonite, xanthan gum and the like.
  • the compounds of formula (I) can also be delivered through the oral cavity by sublingual and/or buccal administration. Molded tablets, compressed tablets or freeze- dried tablets are exemplary forms which may be used.
  • compositions include those formulating the present compound(s) with fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins. Also included in such formulations may be high molecular weight excipients such as celluloses (avicel) or polyethylene glycols (PEG). Such formulations can also include an excipient to aid mucosal adhesion such as hydroxy propyl cellulose (HPC), hydroxy propyl methyl cellulose (HPMC), sodium carboxy methyl cellulose (SCMC), maleic anhydride copolymer (e.g., Gantrez), and agents to control release such as polyacrylic copolymer (e.g. Carbopol 934).
  • fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins.
  • high molecular weight excipients such as celluloses (avicel) or polyethylene glycols (P
  • Lubricants, glidants, flavors, coloring agents and stabilizers may also be added for ease of fabrication and use.
  • Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like.
  • the oral drug components can be combined with any oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like.
  • the compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles.
  • Liposomes can be formed from a variety of phospholipids, 1,2-dipalmitoylphosphatidylcholine, phosphatidyl ethanolamine (cephaline) , or phosphatidylcholine (lecithin).
  • Formulations for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents.
  • the formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilised) condition requiring only the addition of the sterile liquid carrier, for example saline or water-for-injection, immediately prior to use.
  • compositions for parenteral administration include injectable solutions or suspensions which can contain, for example, suitable non-toxic, parenterally acceptable diluents or solvents, such as mannitol, 1,3- butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid, or Cremaphor.
  • suitable non-toxic, parenterally acceptable diluents or solvents such as mannitol, 1,3- butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid, or Cremaphor.
  • compositions for nasal, aerosol or inhalation administration include solutions in saline, which can contain, for example, benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, and/or other solubilizing or dispersing agents such as those known in the art.
  • Formulations for rectal administration may be presented as a suppository with the usual carriers such as cocoa butter, synthetic glyceride esters or polyethylene glycol. Such carriers are typically solid at ordinary temperatures, but liquefy and/or dissolve in the rectal cavity to release the drug.
  • Formulations for topical administration in the mouth include lozenges comprising the active ingredient in a flavoured basis such as sucrose and acacia or tragacanth, and pastilles comprising the active ingredient in a basis such as gelatin and glycerine or sucrose and acacia.
  • exemplary compositions for topical administration include a topical carrier such as Plastibase (mineral oil gelled with polyethylene).
  • Preferred unit dosage formulations are those containing an effective dose, as hereinbefore recited, or an appropriate fraction thereof, of the active ingredient.
  • formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents.
  • a compound of the invention may be used as the sole active ingredient in a medicament, it is also possible for the compound to be used in combination with one or more further active agents.
  • Such further active agents may be further compounds according to the invention, or they may be different therapeutic agents, for example an antidepressant, an anxiolytic, an anti-psychotic, an agent useful in the prevention or treatment of osteoporosis, an agent useful in the prevention or treatment of cancer or other pharmaceutically active material.
  • the compounds of the instant invention may be effectively administered in combination with effective amounts of other agents such as an antidepressant, an anxiolytic, an anti-psychotic, an organic bisphosphonate or a cathepsin K inhibitor.
  • the compounds of the invention may be effectively administered in combination with an effective amount of an antidepressant.
  • antidepressants include noradrenaline reuptake inhibitors (NRI), selective serotonin reuptake inhibitors, monoamine oxidase inhibitors, tricyclic antidepressants (TCA), dopamine reuptake inhibitors (DRI), opioids, selective seretonic reuptake enhancers, tetracyclic antidepressants, reversible inhibitors of monoamine oxidase, melatonin agonists, serotonin and noradrenaline reuptake inhibitors (SNRI), corticotropin releasing factor antagonists, ⁇ - adrenoreceptor antagonists, 5HTl ⁇ receptor agonists and antagonists, lithium and atypical anti- psychotics.
  • NRI noradrenaline reuptake inhibitors
  • TCA tricyclic antidepressants
  • DRI dopamine reuptake inhibitors
  • opioids selective sere
  • antidepressants of the SSRI class include Fluoxetine and Sertraline; examples of antidepressants of the SNRI class Venlafaxine, Citalopram, Paroxetine, Escitalopram, Fluvoxamine; examples of antidepressants of the SNRI class include Duloxetine; examples of antidepressants of the DRI and NRI classes include Bupropion; examples of antidepressants of the TCA class include Amitriptyline and Dothiepin (Dosulepin). Examples of atypical antipsychotics include: Clozapine,
  • Olanzapine, Risperidone, Quetiapine, Ziprasidone and Dopamine partial agonists include benzodiazepines and non-benzodiazapines.
  • benzodiazepines include lorazepam, alprazolam, and diazepam.
  • non-benzodiazapines include Buspirone (Buspar ® ), barbiturates and meprobamate.
  • Buspirone Buspirone (Buspar ® ), barbiturates and meprobamate.
  • One or more of those further anti-depressants may be used in combination.
  • anti-cancer agents examples include tamoxifene or an aromatase inhibitor, used in treatment of breast cancer.
  • a compound of the invention may be used in combination therapy with the agent of such treatment.
  • combination treatment therapies include: a compound of the invention in combination with tamoxifene treatment of breast cancer, a compound of the invention in combination with aromatase inhibitor treatment of breast cancer or a compound of the invention in combination with raloxifene treatment of osteoporosis.
  • Nonlimiting examples of above-mentioned organic bisphosphonates include adendronate, clodronate, etidronate, ibandronate, incadronate, minodronate, neridronate, risedronate, piridronate, pamidronate, tiludronate, zoledronate, pharmaceutically acceptable salts or esters thereof, and mixtures thereof.
  • Preferred organic biphosphonates include alendronate and pharmaceutically acceptable salts and mixtures thereof. Most preferred is alendronate monosodium trihydrate.
  • the precise dosage of the bisphosphonate will vary with the dosing schedule, the oral potency of the particular bisphosphonate chosen, the age, size, sex and condition of the mammal or human, the nature and severity of the disorder to be treated, and other relevant medical and physical factors. Thus, a precise pharmaceutically effective amount cannot be specified in advance and can be readily determined by the caregiver or clinician. An appropriate amount can be determined by routine experimentation from animal models and human clinical studies. Generally, an appropriate amount of bisphosphonate is chosen to obtain a bone resorption inhibiting effect, i.e. a bone resorption inhibiting amount of the bisphonsphonate is administered.
  • an effective oral dose of bisphosphonate is typically from about 1.5 to about 6000 ⁇ g/kg of body weight and preferably about 10 to about 2000 ⁇ g/kg of body weight.
  • a unit dosage typically comprises from about 8.75 mg to about 140 mg of the alendronate compound, on an alendronic acid active weight basis, i.e. on the basis of the corresponding acid.
  • the compounds of the present invention can be used in combination with other agents useful for treating estrogen-mediated conditions.
  • the individual components of such combinations can be administered separately at different times during the course of therapy or concurrently in divided or single combination forms.
  • the present invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment and the term "administering" is to be interpreted accordingly. It will be understood that the scope of combinations of the compounds of this invention with other agents useful for treating estrogen-mediated conditions includes in principle any combination with any pharmaceutical composition useful for treating disorders related to estrogen functioning.
  • the compounds of formula (I) When combined with an antidepressant, an anxiolytic, an anti-psychotic, an organic bisphosphonate or a cathepsin K inhibitor, the compounds of formula (I) may be employed in a weight ratio to the additional agent within the range from about 10:1 to about 1 :10.
  • the compounds of the invention as described above also find use, optionally in labelled form, as a diagnostic agent for the diagnosis of conditions associated with malfunction of the estrogen receptor.
  • a diagnostic agent for the diagnosis of conditions associated with malfunction of the estrogen receptor.
  • such a compound may be radioactively labelled.
  • the compounds of the invention as described above, optionally in labelled form, also find use as a reference compound in methods of discovering other agonists, partial agonists, antagonists or partial antagonists of the estrogen receptor.
  • the invention provides a method of discovering a ligand of the estrogen receptor which comprises use of a compound of the invention or a compound of the invention in labelled form, as a reference compound.
  • a method may involve a competitive binding experiment in which binding of a compound of the invention to the estrogen receptor is reduced by the presence of a further compound which has estrogen receptor-binding characteristics, for example stronger estrogen receptor-binding characteristics than the compound of the invention in question.
  • Examples 46-159 and 211 - full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 46-159 and 211 are described in Examples 1-5, 16 and 38-40.
  • Examples 160-162 and 196-210 - full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 160-162 and 196-210 are described in Examples 1-4, 8, 16 and 38-40.
  • Examples 169 and 173-195 full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 169 and 173-195 are described in Examples 1-4, 8, 10-13, 14-16, 20, 25, 38-40 and 45.
  • Example 1 To 2-bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (Example 1) was added 2 eq 3-thiopheneboronic acid, 2.1 eq potassium carbonate and 10 mol % tetrakis(triphenylphosphine) palladium. THF: EtOH: H 2 O (4:1:0.5) was added and the vial was flushed with nitrogen, sealed and stirred at 100 0 C for 48 hours. The reaction mixture was cooled to rt, diluted with H 2 O, extracted with EtOAc and filtered through silica. The organic phase was evaporated to dryness and the crude product was subjected to reversed phase preparative HPLC.
  • Example 1 2-Bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (Example 1) was mixed with excess dimethylamine in DMF (1:3) and the mixture was stirred in a sealed vial at 80 0 C over night. The reaction-mixture was cooled to rt, diluted with H 2 O and DCM and then the phases were separated. The organic phase was evaporated to dryness and subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by 1 H-NMR and LC/MS. Purity was determined by analytical HPLC.
  • the mixture was heated at 160 0 C during 30min in the microwave.
  • the solvent was co-evaporated with toluene in vacuo.
  • the residue dissolved in CH 2 CI 2 and filtered through a silica plug. The solvent was removed and the mixture obtained was used directly in the next step without further purification.
  • Example 51 2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (Example 51) was dissolved in a 1 :2 mixture of THF and LiOH (aq, 2 M) and run in a microwave at 160 0 C for one hour. The mixture was diluted with H 2 O and DCM, acidified to pH 1 with HCl (1 M) and the phases separated. The organic phase was concentrated in vacuo and purified with Chromatotrone using 5 % MeOH in DCM as mobile phase.
  • Example 103 To 2-bromo-l-(2,3-difluoro-4-hydroxyphenyl)-lH-indole-3-carbonitrile (Example 103) was added 1.5 eq 3,5-dimethylisooxazole-4-boronic acid, 4 eq potassium carbonate, 2 eq sodium iodide and 10 mol% tetrakis(triphenylphosphine)palladium.
  • DME:H 2 O (1 :1) was added and the vial was flushed with nitrogen, sealed and stirred at 150 0 C for 10 min. The reaction mixture was cooled to it, diluted with H 2 O and extracted with DCM.
  • Example 27 4-(3-(Dihydroxyamino)-2-(3,5-dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol (E27)
  • «-BuLi 1.6 M in hexane, 1.2 eq
  • a mixed solvent l-methyl-2- pyrrolodinone and 1 ,4-dioxan, 2:1
  • the mixture was degassed by freeze-pump-thaw twice and then flushed with argon.
  • the mixture was heated to 150°C/15 min. in a Biotage Initiator micro wave oven.
  • the crude product was filtered followed by evaporation and filtration through a short silica gel plug (EtOAc).
  • Purification by flash chromatography (EtOAc/heptane; 1 :9 to 2:8) gave 22.2 mg as a mixture of debrominated starting material and product plus a small amount of unreacted starting material according to LCMS.
  • the compounds separated well on HPLC but only one spot on TLC.
  • the crude material was used directly in the following demethylation step.
  • Example 43 4-(3-chloro-2-(3,5-dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol (E43)
  • R 1 phenyl
  • R 1 methyl
  • R . r 1 _ 1 -methyl-1 H-pyrrol-2-yl
  • R 1 ethynyl
  • R 2-methyl-allyl ES/MS m/z: 289.1 (pos. M + H), 287.1 (neg. M - H); 1 H NMR (acetone-d6, 500MHz): 7.67 (m, IH), 7.31-7.24 (m, 4H), 7.10-7.05 (m, 3H), 4.76 (s, IH), 4.41 (s, IH), 3.55 (s, 2H) and 1.66 (s, 3H).
  • R 1 (Z)-propenyl
  • R 1 Butyl-methyl-amino
  • R 3,5-dimethyl-pyrrazol-l-yl ES/MS m/z: 329.3(pos. M + H), 327.4 (neg. M - H); ⁇ NMR (acetone-d6, 500MHz): 7.81 (m, IH), 7.46-7.42 (m, 2H), 7.35 (m, IH), 7.21 (m, 2H), 6.93 (m, 2H), 5.98 (s, IH), 2.15 (s, 3H) and 2.12 (s, 3H).
  • R 1 2-methoxy-thiazol-4-yl
  • R' 3-methyl-but-2-enyl
  • R 1 4-methyl-thiophen-3 -yl
  • R 1 l,3,5-trimethyl-lH-pyrrazol-4-yl
  • R 1 phenyl
  • R 2-fluoro-4-hydroxy-phenyl
  • R 1 -methyl- 1 H-pyrrol-2-yl
  • R 3, 5 -difluoro-4 -hydroxy-phenyl
  • R 1 -methyl- lH-pyrrazol-4-yl
  • R 3,5-difluoro-4-hydroxy-phenyl ES/MS m/z: 351.5 (pos. M + H), 349.3 (neg. M - H); 1 H NMR (acetone-d6, 500MHz): 7.79 (s, IH), 7.68 (m, IH), 7.50 (s, IH), 7.35-7.28 (m, 2H), 7.25 (m, 2H), 7.18 (m, IH) and 3.91 (s, 3H).
  • R 1 3-methyl-thiophen-2-yl
  • R 3-chloro-4-hydroxy-phenyl
  • R 1 3-methyl-thiophen-2-yl
  • R 3-fluoro-4-hydroxy-plienyl
  • R (E)-pro ⁇ enyl
  • R 1 3-methyl-thiophen-2-yl
  • R 2,3-difluoro-4-hydroxy-phenyl
  • R 1 l-methyl-lH-pyrrol-2-yl
  • R 2,3-difluoro-4-hydroxy-phenyl
  • R prop-1 -ynyl
  • R 2,3-difluoro-4-hydroxy-phenyl ES/MS m/z: 309.1 (pos. M + H), 307.2 (neg. M - H); 1 H NMR (CDCl 3 , 500MHz): 7.74 (m, IH), 7.34- 7.30 (m, 2H), 7.12 (m, IH), 7.05 (m, IH), 6.98 (m, IH) and 2.08 (s, 3H).
  • Examples 160-162 were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 160-162 are described in Examples 1-4, 8, 16 and 38-40 above.
  • Examples 163-168 and 170-172 below were prepared according to General Method 4 above. Full experimental details of the individual steps of that general method are described in Examples 2-4, 9, and 20-21 above. Examples 173-195 below were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 173-195 are described in Examples 1-4, 10-11, 14-16, 38-40 and 45 above.
  • R 1 phenyl
  • R carbamoyl
  • R phenyl
  • R 1 phenyl
  • R 2 thiazol-4-yl
  • R 1 phenyl
  • R prop-l-ynyl
  • R phenyl
  • R 2 pyrrazol-1-yl
  • R 1 phenyl
  • R 2 imidazol-1-yl
  • R pyrrol- 1-yl
  • R N-Hydroxycarbamimidoyl
  • R 1 2-methyl-prop-l-enyl
  • R N-Hydroxycarbamimidoyl
  • R 1 phenyl
  • R hydroxycarbamoyl
  • R pyrrol- 1-yl
  • R t ⁇ r£-butoxycarbonylamino
  • R 1 3,5-Dimethyl-isoxazol-4-yl
  • R 2 (imino)methylacetamide
  • R 5-ethyl-3-methylisoxazol-4-yl
  • R 2 carbamoyl
  • R 1 2-ethyl-lH-pyrrol-l-yl
  • R N-Hydroxycarbamimidoyl
  • R 5-methyl-lH-pyrazol-l-yl
  • R 2 N-Hydroxycarbamimidoyl
  • Examples 196-210 were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 196-210 are described in Examples 1-4, 8, 16 and 38-40 above.
  • R 1 2-methyl-allyl
  • R 4-hydroxy-phenyl
  • R 1 l-methyl-lH-pyrrol-2-yl
  • R 3 -fluoro-4-hydroxy -phenyl
  • Example 211 below was prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Example 211 are described in Examples 1-5, 16 and 38-40 above.
  • Examples 229-232 below were prepared according to General Method 2 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 229-232 are described in Examples 2-4, 8, 16, 24 and 38-40 above.
  • Examples 233-270 below were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 233-270 are described in Examples 1-4, 10-11, 14-16, 38-40 and 45 above.
  • Example 271 was prepared according to General Method 3 above. Full experimental details of the individual steps of that general method are described in Examples 2-4, 8, 16, 24 and 38-40 above.
  • Binding Assay 1 Estrogen Receptor Binding Assay
  • the estrogen receptor ligand binding assays are designed as scintillation proximity assays (SPA), employing the use of tritiated estradiol ( 3 H-E2) and recombinant expressed biotinylated estrogen receptor binding domains.
  • SPA scintillation proximity assays
  • the binding domains of human ERa (ERa-LBD, pET-N-AT #1, aa 301-595) and ER ⁇ (ER ⁇ -LBD, pET-N-AT #1, aa 255-530) proteins are produced in E.coli ((BL21, (DE3), pBirA)) at 22 C in 2xLB medium supplemented with 50 uM biotin.
  • Dilute ER ⁇ -LBD or ER ⁇ -LBD extracts in assay buffer (18 mM K 2 HPO 4 , 2 mM KH 2 PO 4 , 20 mM Na 5 MoO 4 , 1 mM EDTA, ImM TCEP) 1 :676 and 1 :517 for alpha and beta respectively.
  • the diluted receptor concentrations should be 900 fmol/L.
  • Test compounds are evaluated over a range of concentrations from 157 ⁇ M to 37.5 pM.
  • the test compound stock solutions should be made in 100% DMSO at 5x of the final concentration desired for testing in the assay.
  • the amount of DMSO in the test wells of the 384 well plate will be 20%.
  • the Microbeta-instrument generates the mean cpm (counts per minute) value / minute and corrects for individual variations between the detectors thus generating corrected cpm values.
  • Binding Assay 2 Estrogen Receptor Filter Binding Assay
  • the ligand binding domain of the human estrogen receptor beta (hER ⁇ -LBD) is used in a competition binding assay with filter separation of bound and free ligand.
  • the assay utilizes tritiated estradiol ( 3 H-E2) as beta particle emitting tracer and recombinant expressed human estrogen beta receptor binding domain.
  • the binding domain of human ER ⁇ (hER ⁇ -LBD, pET-N-AT #1, aa 255-530) protein is produced in Escherichia coli ((BL21, (DE3), pBirA)) at 22°C in 2xLB medium supplemented with 50 ⁇ M biotin.
  • hER ⁇ -LBD After 3 h of isopropyl ⁇ -D-1-thiogalactopyranoside induction (0.55 mM), cells are harvested by centrifugation at 7300xg for 15 min and cell pellets stored frozen in -20°C. Extraction of hER ⁇ -LBD is performed using 5 g of cells suspended in 50 mL of extraction buffer (50 mM Tris, pH 8.0, 100 mM KCl, 4 mM ethylenediaminetetraacetic acid (EDTA), 4 mM dithiothreitol and 0.1 mM phenylmethanesulfonyl fluoride (TCEP).
  • extraction buffer 50 mM Tris, pH 8.0, 100 mM KCl, 4 mM ethylenediaminetetraacetic acid (EDTA), 4 mM dithiothreitol and 0.1 mM phenylmethanesulfonyl fluoride (TCEP).
  • Estrogen receptor extract is diluted 1 :400 in assay buffer (18 mM K 2 HPO 4 , 2 mM KH 2 PO 4 , 20 mM Na 2 MoO 4 , 1 mM EDTA, ImM TCEP, pH 8.0). Test compounds are evaluated over a range of concentrations from 2 ⁇ M to 10 pM. The test compound stock solutions should be made in 100% dimethyl sulfoxide (DMSO) at 51x of the final concentration desired for testing in the assay.
  • DMSO dimethyl sulfoxide
  • the final fraction of DMSO in the wells of the 96 well assay plate will be 2%.
  • lOO ⁇ l 3 H-E2 is added to the assay plates followed by 4 ⁇ l aliquots of test compounds and lOO ⁇ l of the diluted receptor extract.
  • the assay plates are stored over night at +4°C.
  • Receptor bound and free tracer are separated over a glass fiber filter (FILTERMAT B, PerkinElmer)) on a cell harvester (TOMTECMACH3, Tomtec) with wash buffer (18mM K 2 HPO 4 , 2mM KH 2 PO 4 , 0.5mM EDTA).
  • the filters are dried at 60 0 C for 1 hour and then merged by heat with a scintillating wax (MELTILEX, PerkinElmer) before measuring on a plate beta counter (Wallac Microbeta Trilux 1450- 028, PerkinElmer).
  • the Trilux-instrument generates mean counts per minute (cpm) and corrects for individual variations between the detectors, thus generating corrected cpm values (ccpm).
  • the expression vector pMThER ⁇ contains an insert of wild type human estrogen receptor alpha with deleted leader.
  • the pERE-ALP reporter construct contains the gene for the secreted form of placental alkaline phosphatase (ALP) and the vitellogenin estrogen response element (ERE).
  • ALP placental alkaline phosphatase
  • EAE vitellogenin estrogen response element
  • the human embryonic kidney 293 cells are transfected in two steps. Firstly, a stable clone mix transfected with the pERE-ALP reporter gene construct and pSV2-Neo for selection is developed. Secondly, the stable clone mix is transfected with pMThER ⁇ and a pKSV-Hyg resistance vector for selection. All transfections are performed using Lipofectamine (Invitrogen) according to supplier's recommendations. A selected clone with both pERE-ALP and pMThER ⁇ is used for the transactivation assay.
  • the cells are seeded in 384-well plates at 12 500 cells per well in Ham's F12 Coon's modification (without phenol red) with 10 % dextran-coated charcoal treated (DCC) fetal bovine serum (FBS), 2 niM L-glutamine and 50 ⁇ g/ml gentamicin. After 24 h incubation (37°C, 5 % CO 2 ) the seeding medium is discarded and replaced with 20 ⁇ l Ham's F12 Coon's modification (without phenol red) with 1.5 % DCC- FCS, 2 mM L-glutamine and supplemented with 100 U/ml penicillin and 100 ⁇ g/ml streptomycin.
  • DCC dextran-coated charcoal treated
  • FBS fetal bovine serum
  • FBS fetal bovine serum
  • the selected compounds are added to the wells in 12 concentrations ranging from 3.3 pM to 33 ⁇ M.
  • the compounds are dissolved in 100 % dimethylsulphoxide (DMSO) and the final concentration of DMSO in the assay is 0.1 %.
  • DMSO dimethylsulphoxide
  • the medium is assayed for ALP activity by a chemiluminescence assay; a 10 ⁇ l aliquot of the cell culture medium is mixed with 100 ⁇ l assay buffer (0.1 M diethanolamine, 1 mM MgCl 2 ) and 0.5 mM disodium 3-(4-methoxyspiro l,2-dioxetane-3,2'-(5'- chloro)-tricyclo[3.3.1.13,7]decan-4-yl)phenyl phosphate (CSPD) (Tropix, Applied Biosystems) and incubated for 20 min at 37°C and 15 min at room temperature before measurement chemiluminescent light signal (one second per well) in a Wallac Microbeta Trilux 1450-028 (PerkinElmer).
  • the half maximal effective concentrations (EC 50 ) are calculated from the curves fitted to the concentration- response data with a four parameter logistic model in X
  • Transactivation Assay 2 Transactivation assay in human embryonic kidney 293 cells stably transfected with pERE2-ALP and human estrogen receptor beta
  • the cells were seeded in 384-well plates at 12 500 cells per well in Ham's F12 Coon's modification
  • DMSO dimethylsulfoxide
  • the final concentration of DMSO in the assay was 0.1 %.
  • the medium was assayed for ALP activity by a chemiluminescence assay; a 10 ⁇ l aliquot of the conditioned medium was mixed with 100 ⁇ l assay buffer (0.1 M diethanolamine, 1 mM MgC12) and 0.5 mM disodium 3-(4-methoxyspiro 1,2-dioxetane- 3,2'-(5'-chloro)-tricyclo[3.3.1.13,7]decan-4-yl)phenyl phosphate (CSPD) (Tropix, Applied Biosystems) and incubated for 20 min at 37°C and 15 min at room temperature before measurement of the chemiluminescent signal (one second per well) in a Wallac Microbeta Trilux 1450-028 (PerkinElmer).
  • the ALP activity expressed in LCPS is directly proportional to the level of ALP expressed by the cells.
  • the half maximal effective concentrations of the test compounds (EC50) were calculated from the curves fitted to the concentration-response data with a four parameter logistic model in XLfit software version 2.0 (IDBS) or later.
  • Examples 1-271 exhibit one or more of the following:
  • Example compounds of the invention are those which exhibit a binding affinity to the estrogen receptor ⁇ -subtype at lower concentrations within the IC 5O range shown above.
  • Example compounds of the invention are those which are selective for the estrogen receptor ⁇ - subtype over the estrogen receptor ⁇ -subtype in binding assay 1.
  • the compounds of Examples 2, 4, 5, 11, 23, 43, 51, 53, 68, 70, 39, 95, 133, 134, 143, 146,188, 191, 213, 215, 221, 224, 235 display selectivity for the estrogen receptor ⁇ -subtype of 50 or greater in the binding assay.
  • Example compounds of the invention are those which display a potency at the estrogen receptor ⁇ -subtype at lower concentrations within the EC 50 range shown above.
  • the compounds of Examples 11, 14, 39, 41, 42, 47, 68, 163, 176,187,188, 191, 194, 220, 221, 223, 235-238, 255, 266, 268, 269, 271 exhibit a potency in the range OfEC 50 1 to 50 nM at the estrogen receptor ⁇ -subtype in transactivation assay 2.
  • Example compounds of the invention are those which are selective for the estrogen receptor ⁇ - subtype over the estrogen receptor ⁇ -subtype in the transactivation assays 1 and 2.
  • the compounds of Examples 11, 14, 41, 42, 44, 221, 235-238, 241, 255, 260, 268, 271 display selectivity for the estrogen receptor ⁇ -subtype of 50 or greater in the transactivation assays.
  • Example compounds of the invention are those which exhibit both a binding affinity to the estrogen receptor ⁇ -subtype at lower concentrations within the IC 50 range shown above and a potency at the estrogen receptor ⁇ -subtype at lower concentrations within the EC 50 range shown above.
  • the compounds of Examples 11, 39, 42, 68, 163, 188, 191 , 194, 220, 221, 223, 235 exhibit a binding affinity to the estrogen receptor ⁇ -subtype in the range of IC 50 1 to 200 nM in binding assay 1 and a potency in the range of EC 50 1 to 50 nM at the estrogen receptor ⁇ -subtype in transactivation assay 2.

Abstract

The invention provides a compound of formula (I) or a pharmaceutically acceptable ester, amide, carbamate, solvate or salt thereof, including a salt of such an ester, amide or carbamate, and a solvate of such an ester, amide, carbamate or salt. The invention also provides also provides the use of such compounds in the treatment or prophylaxis of a condition associated with a disease or disorder associated with estrogen receptor activity, wherein R1, R2, R3, R4, R5 and R6 are as defined in the specification.

Description

Novel Estrogen Receptor Ligands
Field of Invention
This invention relates to compounds which are estrogen receptor ligands and are preferably selective for the estrogen receptor β isoform, to methods of preparing such compounds and to methods for using such compounds in treatment of diseases related to the estrogen receptor such as depressive disorders, anxiety disorders, Alzheimer's disease, cognitive disorders, osteoporosis, elevated blood triglyceride levels, atherosclerosis, endometriosis, urinary incontinence, autoimmune disease, and cancer of the lung, colon, breast, uterus and prostate.
Background of Invention
The estrogen receptor (ER) is a ligand activated mammalian transcription factor involved in the up and down regulation of gene expression. The natural hormone for the estrogen receptor is β-17-estradiol (E2) and closely related metabolites. Binding of estradiol to the estrogen receptor causes a dimerization of the receptor and the dimer in turn binds to estrogen response elements (ERE' s) on DNA. The ER/DNA complex recruits other transcription factors responsible for the transcription of DNA downstream from the ERE into mRNA which is eventually translated into protein. Alternatively the interaction of ER with DNA may be indirect through the intermediacy of other transcription factors, most notably fos and jun. Since the expression of a large number of genes is regulated by the estrogen receptor and since the estrogen receptor is expressed in many cell types, modulation of the estrogen receptor through binding of either natural hormones or synthetic ER ligands can have profound effects on the physiology and pathophysiology of the organism.
Historically it has been believed there was only one estrogen receptor. However a second subtype (ER-β) has been discovered. While both the "classical" ER-α and the more recently discovered ER-β are widely distributed in different tissues, they nevertheless display markedly different cell type and tissue distributions. Therefore synthetic ligands which are either ER-α or ER-β selective may preserve the beneficial effects of estrogen while reducing the risk of undesirable side effects.
Estrogens are critical for sexual development in females. In addition, estrogens play an important role in maintaining bone density, regulation of blood lipid levels, and appear to have neuroprotective effects. Consequently decreased estrogen production in post-menopausal women is associated with a number of diseases such as osteoporosis, atherosclerosis, depression and cognitive disorders. Conversely certain types of proliferative diseases such as breast and uterine cancer and endometriosis are stimulated by estrogens and therefore antiestrogens (i.e., estrogen antagonists) have utility in the prevention and treatment of these types of disorders. The efficacy of the natural estrogen, 17β-estradiol, for the treatment of various forms of depressive illness has also been demonstrated and it has been suggested that the anti-depressant activity of estrogen may be mediated via regulation of tryptophan hydroxylase activity and subsequent serotonin synthesis (See, e.g., Lu N Z, Shlaes T A, Cundlah C, Dziennis S E, LyIe R E, Bethea C L, "Ovarian steroid action on tryptophan hydroxylase protein and serotonin compared to localization of ovarian steroid receptors in midbrain of guinea pigs." Endocrine 11 :257-267, 1999). The pleiotropic nature of natural estrogen precludes its widespread, more chronic use due to the increased risk of proliferative effects on breast, uterine and ovarian tissues. The identification of the estrogen receptor, ERβ, has provided a means by which to identify more selective estrogen agents which have the desired anti-depressant activity in the absence of the proliferative effects which are mediated by ERa. Thus, it has been shown that therapeutic agents having ERβ-selectivity are potentially effective in the treatment of depression.
What is needed in the art are compounds that can produce the same positive responses as estrogen replacement therapy without the negative side effects. Also needed are estrogen-like compounds that exert selective effects on different tissues of the body.
WO 2006/019831 discloses certain indole derivatives having utility in the prevention or treatment of Hepatitis C viral infection. WO 2005/018636 discloses certain indole derivative having estrogen receptor modulator activity, all said indoles being oximes.
The compounds of the present invention are ligands for estrogen receptors and as such may be useful for treatment or prevention of a variety of conditions related to estrogen functioning including bone loss, bone fractures, osteoporosis, cartilage degeneration, endometriosis, uterine fibroid disease, hot flashes, increased levels of LDL cholesterol, cardiovascular disease, impairment of cognitive functioning, age- related mild cognitive impairment, cerebral degenerative disorders, restenosis, gynecomastia, vascular smooth muscle cell proliferation, obesity, incontinence, anxiety, depression, perimenopausal depression, post-partum depression, premenstrual syndrome, manic depression, dementia, obsessive compulsive behavior, attention deficit disorder, attention deficit hyperactivity disorder, sleep disorders, irritability, impulsivity, anger management, hearing disorders, multiple sclerosis, Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, spinal cord injury, stroke, autoimmune disease, inflammation, IBD, IBS, sexual dysfunction, hypertension, retinal degeneration, lung cancer, colon cancer, breast cancer, uterus cancer, prostate cancer and cholangiocarcinoma. Summary of the Invention
This invention provides a compound of formula (I) or a pharmaceutically acceptable ester, amide, carbamate, solvate or salt thereof, including a salt of such an ester, amide or carbamate, and a solvate of such an ester, amide, carbamate or salt,
Figure imgf000004_0001
(I)
wherein R1 is selected from the group consisting of halogen, cyano, nitro, ORΛ, N(RB)2, -C(O)Ci_4alkyl, -SO2CMalkyl, C1-6alkyl, C2-6alkenyl, C2-6alkynyl, haloCi_6alkyl, dihaloCi-6alkyl, trihaloCi_6alkyl, haloC2_ 6alkenyl, dihaloC2_6alkenyl, trihaloC2-6alkenyl, cyanoCi_6alkyl, Ci_4alkoxyCi.6 alkyl, C3.gcycloalkyl, C3_8cycloalkylCi.6 alkyl, phenyl, benzyl, and 5-10 membered heterocyclyl, wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents, each substituent being selected from the group consisting of ORΛ, halogen, cyano, nitro, -C(O)C1.4alkyl, Cj- βalkyl, C2.6alkenyl, C2-6alkynyl, haloCi_6 alkyl, dihaloC^alkyl and trihaloCi_6alkyl;
R2 is selected from the group consisting of halogen, cyano, nitro, ORA, N(RB)2, N(OH)2, -C(O)Ci_4alkyl optionally substituted with from 1 to 3 halogens, -SO2CMalkyl, -C(O)NH-OH, -C(NH2)=N-0H, -C(CO2H)=N-OH, -C(NH2)=NH, -C(NH CMalkyl)=NH, -C(O-C Malkyl^NH, -C(NH2)=N-NH2, -NH-C(NH2)=NH, -NH-C(O)NH2, -N=C(-NH-CH2CH2-NH-), -S-CN, -S-C(NH2)=NH, -S-C(NH2)=N- OH, -CO2H, -CH2-CO2H, -CH(OH)CO2H, -C(O)CO2H, SO3H, CH2SO3H, C^alkyl, haloCi-6alkyl, dihaloCi_6alkyl, trihaloC^alkyl, cyanoCi_6alkyl, Ci_4alkoxyCi.6 alkyl, C2_6alkenyl, C2_6alkynyl, C3. gcycloalkyl, C3_8cycloalkylCi.6 alkyl, phenyl, benzyl and 5-10 membered heterocyclyl wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents each substituent being selected from the group consisting of 0RΛ, halogen, cyano, nitro,
Ci_6alkyl, C2-6alkenyl, C2.6alkynyl, haloCi_6alkyl, dihaloC^alkyl and trihaloC^alkyl; provided that if one of R1 and R2 represents halogen, the other must represent a group other than halogen;
each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen, 0RA, halogen, cyano, nitro, Ci.6alkyl, C2.6alkenyl, C2.6alkynyl, haloCi_6alkyl, dihaloCi_6alkyl and trihaloC^alkyl; each RA is independently selected from the group consisting of hydrogen, Ci_6alkyl, C2_6alkenyl, C2. βalkynyl, C3_8cycloalkyl, C3_8cycloalkylCi_6alkyl, Cβ-ioaryl and C6_io
Figure imgf000005_0001
each optionally substituted by from 1 to 3 halogen atoms; and
each RB is independently selected from the group consisting of hydrogen, Ci_6alkyl, C2_6alkenyl, C2. 6alkynyl, Cj_8cycloalkyl, C3_gcycloalkylCi_6alkyl, Cβ-ioaryl and C640arylCi_6alkyl, each optionally substituted by from 1 to 3 halogen atoms;
with the proviso that the compound of formula (I) is not 4-[3-(4,5-Dihydro-lH-imidazol-2-yl)-2-(3,5-dimethyl-isoxazol-4-yl)-indol-l -yl]-phenol; l-(4-Hydroxy-phenyl)-2-(4-methyl-imidazol-l-yl)-lH-indole-3-carbonitrile;
1 -(4-Hydroxy-phenyl)-2-( 1 H-pyrazol-3 -yl)- 1 H-indole-3-carbonitrile; l-(3-Chloro-4-hydroxy-phenyl)-2-(l-methyl-lH-pyrazol-4-yl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-prop-l-ynyl-lH-indole-3-carboxylic acid amide; or 1 -(4-Hydroxy-phenyl)-2-thiazol-2-yl- 1 H-indole-3 -carboxylic acid.
Compounds of the invention have surprisingly been found to be ligands of the estrogen receptor. The compounds accordingly have use in the treatment or prophylaxis of conditions associated with estrogen receptor activity.
Detailed Description of Invention
The compounds of the invention may contain chiral (asymmetric) centers or the molecule as a whole may be chiral. The individual stereoisomers (enantiomers and diastereoisomers) and mixtures of these are within the scope of the present invention.
The present invention provides compounds that are estrogen receptor ligands. The term "estrogen receptor ligand" as used herein is intended to cover any moiety which binds to an estrogen receptor. The ligand may act as an agonist, a partial agonist, an antagonist or a partial antagonist. The ligand may be ERβ selective or display mixed ERa and ERβ activity. For example, the ligand may act both as an agonist or a partial agonist of ERβ and as an antagonist or a partial antagonist of ERa.
When R1 represents a heterocyclyl group, this group may be saturated or unsaturated, and may contain one or more O, N and/or S atoms. It is preferably 5- or 6-membered. Suitable heterocyclyl groups include furyl, thienyl, pyrrolyl, pyrrolinyl, pyrrolidinyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, imidazolyl, imidazolinyl, imidazolidine, pyrazoly, pyrazolinyl, pyrazolidinyl, pyridyl, morpholinyl, and piperidyl, with isoxazolyl being a particularly preferred heterocyclyl group. Preferred substituents for a heterocyclyl group include 1 to 3, for example 1 or 2, substituents, each substituent being selected from the group consisting of ORA, halogen, cyano, -C(O)C].4alkyl, CMalkyl, C2-4alkenyl, C2.4alkynyl, haloCj. 4alkyl, dihaloCi_4alkyl and
Figure imgf000006_0001
Especially preferred substituents are selected from halogen, cyano, Ci_4alkyl (especially methyl), -C(O)Ci_4alkyl, and ORA in which RA preferably represents a hydrogen atom or a
Figure imgf000006_0002
group. More especially preferred substituents are selected from halogen, cyano and CMalkyl (especially methyl or ethyl).
Preferred substituents for a phenyl or benzyl group R include those mentioned above for a heterocyclyl group R1.
When R2 represents a heterocyclyl group, this group may for example be one of the preferred groups mentioned above for R1.
Preferred substituents for a phenyl or benzyl group R2 include those mentioned above for a heterocyclyl group R1.
Unless otherwise stated, each RA is preferably independently selected from the group consisting of hydrogen, Ci_4alkyl, C2-4alkenyl, C2_4alkynyl, C3_6cycloalkyl, phenyl and benzyl. Preferably each RA independently represents hydrogen or Ci_4alkyl, especially methyl.
Unless otherwise stated, each RB is preferably independently selected from the group consisting of hydrogen and Ci_4alkyl.
Preferably R1 is selected from the group consisting of ORA, N(RB)2, -C(O)C ,.4alkyl, C,.6alkyl, C2. 6alkenyl, C2.6alkynyl, haloCMalkyl, dihaloCi_4alkyl, trihaloCi_4alkyl, haloC2.4alkenyl, dihaloC2.4alkenyl, trihaloC2_4alkenyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted as above. More preferably, R1 is selected from the group consisting of ORA, N(RB)2, -C(O)CMalkyl, C,.4alkyl, C2.4alkenyl, C2.4alkynyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted as above.
R2 may for example be selected from one of the preferred groups mentioned above for R1. In one embodiment of the invention, R2 is selected from the group consisting of halogen, cyano, nitro, ORA, N(RB)2, N(OH)2, -C(O)CMalkyl optionally substituted with from 1 to 3 halogens, -SO2C,.4alkyl, -C(O)NH-OH, -C(NH2)=N-0H, -C(CO2H)=N-OH, -C(O-C Malkyl)=NH, -C(NH2)=N- NH2,
-NH-C(NH2)=NH, -NH-C(O)NH2, -N=C(-NH-CH2CH2-NH-), -S-CN, -S-C(NH2)=NH, -S-C(NH2)=N- OH, -CO2H, -CH2-CO2H, -CH(OH)CO2H, -C(O)CO2H, SO3H, CH2SO3H, C,.6alkyl, haloC^alkyl, dihaloCi-6alkyl, trihaloCi_6alkyl, cyanoCi-6alkyl,
Figure imgf000007_0001
alkyl, C2-6alkenyl, C2_6alkynyl, C3. scycloalkyl, C3-scycloalkylCi_6 alkyl, phenyl, benzyl and 5-10 membered heterocyclyl wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents each substituent being selected from the group consisting of ORA, halogen, cyano, nitro, Ci_6alkyl, C2_6alkenyl, C2_6alkynyl, haloCi_6alkyl,
Figure imgf000007_0002
In an alternative embodiment of the invention, R2 is selected from the group consisting of halogen, nitro, ORA, N(RB)2, -C(O)Ci-4alkyl optionally substituted with from 1 to 3 halogens, -SO2CMalkyl, -C(O)NH- OH, -C(NH2)=N-0H, -C(NH2)=NH, -NH-C(NH2)=NH, -NH-C(O)NH2, -N=C^NH-CH2CH2-NH-), -S-C(NH2)=NH, -CO2H, -CH2-CO2H, SO3H, CH2SO3H, C,_6alkyl, haloCi.6alkyl, dihaloC^alkyl, trihaloC].6alkyl, cyanoCi_6alkyl, Ci_4alkoxyCi_6 alkyl, C2-6alkenyl, C2.6alkynyl, C3-gcycloalkyl, C3.8cycloalkylCi.6 alkyl, phenyl, benzyl and 5-10 membered heterocyclyl wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents each substituent being selected from the group consisting of 0RA, halogen, cyano, nitro, C^alkyl, C2_6alkenyl, C2_6alkynyl, haloCi.6alkyl, dihaloCi_6alkyl and trihaloCi_6alkyl.
In a preferred embodiment of the invention, R2 is selected from the group consisting of halogen, nitro, 0RA, N(RB)2, -C(O)Ci.4alkyl optionally substituted with from 1 to 3 halogens, -SO2C1-4alkyl, -C(O)NH- OH, -C(NH2)=N-0H, -NH-C(NH2)=NH, -NH-C(O)NH2, -N=C(-NH-CH2CH2-NH-), -S-C(NH2)=NH, -CO2H, -CH2-CO2H, C,_6alkyl, haloC^alkyl, dihaloCi-6alkyl, trihaloCi-6alkyl, cyanoCi.6alkyl,
Ci_4alkoxyCi_6 alkyl, C2.6alkenyl, C2.6alkynyl, C3.8cycloalkyl, C3_8cycloalkylC1.6 alkyl, phenyl, benzyl and 5-10 membered heterocyclyl wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents each substituent being selected from the group consisting of 0RA, halogen, cyano, nitro, Ci_6alkyl, C2_6alkenyl, C2.ealkynyl, haloC]_6alkyl, dihaloCi_6alkyl and trihaloCi_6alkyl. More preferably, R2 is selected from the group consisting of -C(O)C i_4alkyl
(especially -C(O)CH3), -C(NH2)=N-0H, -CO2H, -CH2-CO2H, CMalkyl, C2.4alkenyl, C2.4alkynyl, and 5-6 membered heterocyclyl wherein said heterocyclyl group can either be unsubstituted or substituted as above. Most preferably, R2 is selected from the group consisting of -C(O)CH3), -C(NH2)=N-0H, -CO2H, and -CH2-CO2H, with -C(NH2)=N-0H being a particularly preferred R2 group.
Preferably each of R3, R4, R5 and R6 is selected from the group consisting of hydrogen, 0RΛ, halogen, cyano, Ci.4alkyl, for example methyl, haloCi_4alkyl, for example chloro- or fiuoro-methyl, dihaloCi. 4alkyl, for example di chloro- or difluoromethyl, and trihaloCi_4alkyl, for example trichloro- or trifluoromethyl. Preferably each of R3, R4, R5 and R6 is selected from the group consisting of hydrogen, OH, halogen, cyano, methyl, or trifluoromethyl. Most preferably at least one of R3 and R4 is hydrogen.
Most preferably each of R5 and R6 independently represents hydrogen and/or halogen, especially fluorine. Accordingly, in one preferred group of compounds of the invention, R1 is selected from the group consisting of ORA, N(RB)2, -C(O)CMalkyl, CI-6alkyl, C2.6alkenyl, C2.6alkynyl, haloCi-4alkyl, dihaloQ. 4alkyl,
Figure imgf000008_0001
haloC2-4alkenyl, dihaloC2_4alkenyl, trihaloC2_4alkenyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group may be either unsubstituted or substituted as above; more preferably, R1 is selected from the group consisting of ORA, N(RB)2, -C(O)Ci_4alkyl, Ci^alkyl, C2-4alkenyl, C2_4alkynyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted as above;
R2 is selected from the group consisting of -C(O)C Malkyl (especially -C(O)CH3), -C(NH2)=N-0H, -CO2H, -CH2-CO2H, Ci_4alkyl, C2_4alkenyl, C2_4alkynyl, and 5-6 membered heterocyclyl wherein said heterocyclyl group can be either unsubstituted or substituted as above;
each of R3, R4, R5 and R6 is selected from the group consisting of hydrogen, 0RA, halogen, cyano, Ci_4alkyl, haloCi_4alkyl, dihaloCi.4alkyl, and trihaloCi_4alkyl, especially hydrogen, OH, halogen, cyano, methyl, or trifluoromethyl; especially, each of R5 and R6 represents hydrogen and/or halogen, especially fluorine;
each RA is preferably independently selected from the group consisting of hydrogen,
Figure imgf000008_0002
C2_ 4alkenyl, C2.4alkynyl, C3_6cycloalkyl, phenyl and benzyl, especially hydrogen and Ci_4alkyl, especially methyl; and
each RB is independently selected from the group consisting of hydrogen and C,_4alkyl.
In a further embodiment,this invention provides a compound of formula (I) or a pharmaceutically acceptable ester, amide, solvate or salt thereof, including a salt of such an ester or amide, and a solvate of such an ester, amide or salt,
Figure imgf000008_0003
(I)
wherein R1 is selected from the group consisting of halogen, cyano, nitro, 0RA, N(RB)2, -C(O)Ci_4alkyl, -SO2C|.4alkyl, Ci_6alkyl, C2-6alkenyl, C2.6alkynyl, haloCi_&alkyl, dihaloCi_6alkyl, trihaloCi_6alkyl, haloC2. 6alkenyl, dihaloC2-6alkenyl, trihaloC2-6alkenyl, cyanoCi_6alkyl, Ci_4alkoxyCi_6 alkyl, C^cycloalkyl, C3.8cycloalkylCi_6 alkyl, phenyl, benzyl, and 5-10 membered heterocyclyl, wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents, each substituent being selected from the group consisting of ORA, halogen, cyano, nitro,
Figure imgf000009_0001
Ci- βalkyl, C2_6alkenyl, C2-6alkynyl, haloCi-6 alkyl, dihaloC^alkyl and trihaloCi_6alkyl;
R2 is selected from the group consisting of halogen, cyano, nitro, ORA, N(RB)2, -C(O)Ci.4alkyl optionally substituted with from 1 to 3 halogens, -SO2CMalkyl, -C(O)NH-OH, -C(NH2)=N-0H, -C(NH2)=NH, -NH-C(NH2)=NH, -NH-C(O)NH2, -N=C(-NH-CH2CH2-NH-), -S-C(NH2)=NH, -CO2H, -CH2-CO2H, SO3H, CH2SO3H, Ci_6alkyl, haloCi-6alkyl, dihaloCi-6alkyl, trihaloC]-6alkyl, cyanoCi-6alkyl, CMalkoxyCi-6 alkyl, C2-6alkenyl, C2_6alkynyl, C3.8cycloalkyl, C3_8cycloalkylCi.6 alkyl, phenyl, benzyl and 5-10 membered heterocyclyl wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents each substituent being selected from the group consisting of 0RA, halogen, cyano, nitro, Ci_6alkyl, C2-6alkenyl, C2_6alkynyl, haloCi_6alkyl, dihaloCi_βalkyl and trihaloC^alkyl; provided that if one of R1 and R2 represents halogen, the other must represent a group other than halogen;
each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen, 0RA, halogen, cyano, nitro, Ci_6alkyl, C2.6alkenyl, C2_6alkynyl, haloCi_6alkyl, dihaloCi_6alkyl and trihaloCi_6alkyl;
each RΛ is independently selected from the group consisting of hydrogen, C]_6alkyl, C2.6alkenyl, C2. 6alkynyl, C3_8cycloalkyl, C3_8cycloalkylCi_6alkyl, Cδ-ioaryl and C6_io arylCi_6alkyl, each optionally substituted by from 1 to 3 halogen atoms; and
each RB is independently selected from the group consisting of hydrogen, Ci_6alkyl, C2.6alkenyl, C2. βalkynyl, C3_8cycloalkyl, Cv8cycloalkylC]_6alkyl, Cβ-ioaryl and C6-io arylCi_6alkyl, each optionally substituted by from 1 to 3 halogen atoms;
with the proviso that the compound of formula (I) is not 4-[3-(4,5-Dihydro-lH-imidazol-2-yl)-2-(3,5- dimethyl-isoxazol-4-yl)-indol-l -yl]-phenol.
Compounds of the formula I include, but are not limited to, the compounds specifically named in the Examples herein. In the Examples, the compound names were generated in accordance with IUPAC by the ACD Labs 8.0/name program, version 8.05 and/or with ISIS DRAW Autonom 2000 and/or ChemBioDraw Ultra version 11.0. Depending upon the substituents present in compounds of the formula I, the compounds may form esters, amides, carbamates and/or salts. Salts and solvates of compounds of formula (I) which are suitable for use in medicine are those wherein a counterion or associated solvent is pharmaceutically acceptable. However, salts and solvates having non-pharmaceutically acceptable counterions or associated solvents are within the scope of the present invention, for example, for use as intermediates in the preparation of the compounds of formula (I) and their pharmaceutically acceptable salts, solvates and physiologically functional derivatives. By the term "physiologically functional derivative" is meant a chemical derivative of a compound of formula (I) having the same physiological function as the free compound of formula (I), for example, by being convertible in the body thereto. Esters, amides and carbamates are examples of physiologically functional derivatives.
Suitable salts according to the invention include those formed with organic or inorganic acids or bases, hi particular, suitable salts formed with acids according to the invention include those formed with mineral acids, strong organic carboxylic acids, such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted, for example, by halogen, such as saturated or unsaturated dicarboxylic acids, such as hydroxycarboxylic acids, such as amino acids, or with organic sulfonic acids, such as (C1- C4)-alkyl- or aryl-sulfonic acids which are unsubstituted or substituted, for example by halogen. Pharmaceutically acceptable acid addition salts include those formed from hydrochloric, hydrobromic, sulphuric, nitric, citric, tartaric, acetic, phosphoric, lactic, pyruvic, acetic, trifluoroacetic, succinic, perchloric, fumaric, maleic, glycolic, lactic, salicylic, oxaloacetic, methanesulfonic, ethanesulfonic, p- toluenesulfonic, formic, benzoic, malonic, naphthalene-2-sulfonic, benzenesulfonic, isethionic, ascorbic, malic, phthalic, aspartic, and glutamic acids, lysine and arginine. Other acids such as oxalic, while not in themselves pharmaceutically acceptable, may be useful as intermediates in obtaining the compounds of the invention and their pharmaceutical acceptable acid addition salts.
Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts, for example those of potassium and sodium, alkaline earth metal salts, for example those of calcium and magnesium, and salts with organic bases, for example dicyclohexylamine, N-methyl-D-glucomine, morpholine, thiomorpholine, piperidine, pyrrolidine, a mono-, di- or tri-lower alkylamine, for example ethyl-, tert-butyl-, diethyl-, diisopropyl-, triethyl-, tributyl- or dimethyl -propylamine, or a mono-, di- or trihydroxy lower alkylamine, for example mono-, di- or triethanolamine. Corresponding internal salts may furthermore be formed.
Compounds of formula (I) may have an appropriate group converted to an ester, an amide or a carbamate. Thus typical ester and amide groups formed from an acid group in the compound of the formula I include -COORB, -CONRB 2, -SO2. ORB, or -SO2.NRB 2, while typical ester and amide and carbamate groups formed from an -OH or -NHRB group in the compound of the formula I include -O.CO.RB, -NRB.CO.RB, -NRB. CO2R8 -O.SO2RB, and -NRB SO2RB, where RB has one of the meanings given above.
Those skilled in the art of organic chemistry will appreciate that many organic compounds can form complexes with solvents in which they are reacted or from which they are precipitated or crystallized. These complexes are known as "solvates". For example, a complex with water is known as a "hydrate".
A compound which, upon administration to the recipient, is capable of being converted into a compound of formula (I) as described above, or an active metabolite or residue thereof, is known as a "prodrug". A prodrug may, for example, be converted within the body, e. g. by hydrolysis in the blood, into its active form that has medical effects. Pharmaceutical acceptable prodrugs are described in T. Higuchi and V. Stella, Prodrugs as Novel Delivery Systems, Vol. 14 of the A. C. S. Symposium Series (1976); "Design of Prodrugs" ed. H. Bundgaard, Elsevier, 1985; and in Edward B. Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987, which are incorporated herein by reference.
The following definitions apply to the terms as used throughout this specification, unless otherwise limited in specific instances.
As used herein, the term "alkyl" means both straight and branched chain saturated hydrocarbon groups.
Examples of alkyl groups include methyl, ethyl, n-propyl, iso-propyl, n-butyl, t-butyl, i-butyl, sec-butyl, pentyl and hexyl groups. Among unbranched alkyl groups, there are preferred methyl, ethyl, n-propyl, iso-propyl, n-butyl groups. Among branched alkyl groups, there may be mentioned t-butyl, i-butyl, 1- ethylpropyl and 1 -ethylbutyl groups.
As used herein, the term "alkoxy" means the group O-alkyl, where "alkyl" is used as described above.
Examples of alkoxy groups include methoxy and ethoxy groups. Other examples include propoxy and butoxy.
As used herein, the term "alkenyl" means both straight and branched chain unsaturated hydrocarbon groups with at least one carbon carbon double bond. Examples of alkenyl groups include ethenyl, propenyl, butenyl, pentenyl and hexenyl. Preferred alkenyl groups include ethenyl, 1- propenyl and 2- propenyl.
As used herein, the term "alkynyl" means both straight and branched chain unsaturated hydrocarbon groups with at least one carbon carbon triple bond. Examples of alkynyl groups include ethynyl, propynyl, butynyl, pentynyl and hexynyl. Preferred alkynyl groups include ethynyl 1- propynyl and 2- propynyl.
As used herein, the term "cycloalkyl" means a saturated group in a ring system. A cycloalkyl group can be monocyclic or bicyclic. A bicyclic group may, for example, be fused or bridged. Examples of monocyclic cycloalkyl groups include cyclopropyl, cyclobutyl and cyclopentyl. Other examples of monocyclic cycloalkyl groups are cyclohexyl, cycloheptyl and cyclooctyl. Examples of bicyclic cycloalkyl groups include bicyclo [2. 2.1]hept-2-yl. Preferably, the cycloalkyl group is monocyclic.
As used herein, the term "aryl" means a monocyclic or bicyclic aromatic carbocyclic group. Examples of aryl groups include phenyl and naphthyl. A naphthyl group may be attached through the 1 or the 2 position. In a bicyclic aromatic group, one of the rings may, for example, be partially saturated. Examples of such groups include indanyl and tetrahydronaphthyl. Specifically, the term C5-I0 aryl is used herein to mean a group comprising from 5 to 10 carbon atoms in a monocyclic or bicyclic aromatic group. A particularly preferred C5-I0 aryl group is phenyl.
As used herein, the term "halogen" means fluorine, chlorine, bromine or iodine. Fluorine, chlorine and bromine are particularly preferred.
As used herein, the term "haloalkyl" means an alkyl group having a halogen substituent, the terms "alkyl" and "halogen" being understood to have the meanings outlined above. Similarly, the term "dihaloalkyl" means an alkyl group having two halogen substituents and the term "trihaloalkyl" means an alkyl group having three halogen substituents. Examples of haloalkyl groups include fluoromethyl, chloromethyl, bromomethyl, fluoromethyl, fluoropropyl and fluorobutyl groups; examples of dihaloalkyl groups include difluoromethyl and difluoroethyl groups; examples of triihaloalkyl groups include trifluoromethyl and trifluoroethyl groups.
As used herein, the term "heterocyclyl" means an aromatic or a non-aromatic cyclic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen or sulfur. A heterocyclyl group may, for example, be monocyclic or bicyclic. In a bicyclic heterocyclyl group there may be one or more heteroatoms in each ring, or only in one of the rings. A heteroatom is preferably O or N. Heterocyclyl groups containing a suitable nitrogen atom include the corresponding N-oxides.
Examples of monocyclic non-aromatic heterocyclyl groups (also referred to as monocyclic heterocycioalkyl rings) include aziridinyl, azetidinyl, pyrrolidinyl, imidazolidinyl, pyrazolidinyl, piperidinyl, piperazinyl, tetrahydrofuranyl, telrahydropyranyl, morpholinyl, thiomorpholinyl and azepanyl.
Examples of bicyclic heterocyclyl groups in which one of the rings is non-aromatic include dihydrobenzofuranyl, indanyl, indolinyl, isoindolinyl, tetrahydroisoquinolinyl, tetrahydroquinolyl and benκoazepanyl.
Examples of monocyclic aromatic heterocyclyl groups (also referred to as monocyclic heteroaryl groups) include furanyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, oxadiazolyl, thiadiazolyl, pyridyl, triazolyl, triazinyl, pyridazyl, isothiazolyl, isoxazolyl, pyrazinyl, pyrazolyl and pyrimidinyl.
Examples of bicyclic aromatic heterocyclyl groups (also referred to as bicyclic heteroaryl groups) include quinoxalinyl, quinazolinyl, pyridopyrazinyl, benzoxazolyl, benzothiophenyl, benzimidazolyl, naphthyridinyl, quinolinyl, benzofuranyl, indolyl, benzothiazolyl, oxazolyl[4,5-b]pyridiyl, pyridopyrimidinyl, isoquinolinyl and benzodroxazole.
Examples of preferred heterocyclyl groups include piperidinyl, tetrahydrofuranyl, tetrahydropyranyl, pyridyl, pyrimidinyl and indolyl. Preferred heterocyclyl groups also include thienyl, thiazolyl, furanyl, pyrazolyl, pyrrolyl, isoxazolyl and imidazolyl.
As used herein the term "cycloalkylalkyl" means a group cycloalkyl-alkyl- attached through the alkyl group, "cycloalkyl" and "alkyl" being understood to have the meanings outlined above.
As mentioned above, the compounds of the invention have activity as estrogen receptor ligands. The compounds of the invention have activity as estrogen receptor modulators, and may be agonists, partial agonists, antagonists, or partial antagonists of the estrogen receptor. Particularly preferred compounds of the invention have activity as an agonist or a partial agonist of ERβ. Preferred compounds of this type are selective agonists of the estrogen receptor-beta (ERβ).
The compounds of the invention may thus be used in the treatment of diseases or disorders associated with estrogen receptor activity. In particular, the compounds of the invention that are agonists or partial agonists of the estrogen receptor may be used in the treatment of diseases or disorders for which selective agonists or partial agonists of the estrogen receptor are indicated. The compounds of the invention that are antagonists or partial antagonists of the estrogen receptor may be used in the treatment of diseases or disorders for which selective antagonists or partial antagonists of the estrogen receptor are indicated. Clinical conditions for which an agonist or partial agonist is indicated include, but are not limited to, bone loss, bone fractures, osteoporosis, cartilage degeneration, endometriosis, uterine fibroid disease, hot flashes, increased levels of LDL cholesterol, cardiovascular disease, impairment of cognitive functioning, cerebral degenerative disorders, restenosis, gynecomastia, vascular smooth muscle cell proliferation, obesity, incontinence, anxiety, depression, autoimmune disease, inflammation, IBD, IBS, sexual dysfunction, hypertension, retinal degeneration, and lung, colon, breast, uterus, and prostate cancer, and/or disorders related to estrogen functioning.
The compounds of the invention find particular application in the treatment or prophylaxis of the following: bone loss, bone fractures, osteoporosis, cartilage degeneration, endometriosis, uterine fibroid disease, hot flashes, increased levels of LDL cholesterol, cardiovascular disease, impairment of cognitive functioning, age-related mild cognitive impairment, cerebral degenerative disorders, restenosis, gynecomastia, vascular smooth muscle cell proliferation, obesity, incontinence, anxiety, depression, perimenopausal depression, post-partum depression, premenstrual syndrome, manic depression, dementia, obsessive compulsive behavior, attention deficit disorder, attention deficit hyperactivity disorder, sleep disorders, irritability, impulsivity, anger management, hearing disorders, multiple sclerosis, Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, spinal cord injury, stroke, autoimmune disease, inflammation, IBD, IBS, sexual dysfunction, hypertension, retinal degeneration, lung cancer, colon cancer, breast cancer, uterus cancer, prostate cancer and the bile duct cancer form named cholangiocarcinoma.
In one embodiment of the invention, the present compounds finds particular application in the treatment or prophylaxis of depression, perimenopausal depression, post-partum depression, premenstrual syndrome and manic depression.
The treatment or prophylaxis of hot flashes (or hot flushes) in males, is preferable for patients that has had an androgen ablation for treatment of prostate cancer.
The phrase "depression" includes but is not limited to, major depressive disorder, dysthymic disorder, bipolar disorder, cyclothymic disorder, mood disorder due to a general medical condition, substance- induced mood misorder, seasonal affective disorder (SAD), postpartum depression and premenstrual dysphoric disorder.
The invention also provides a method for the treatment or prophylaxis of a condition in a mammal mediated by an estrogen receptor, which comprises administering to the mammal a therapeutically effective amount of a compound according to the invention. Clinical conditions mediated by an estrogen receptor that may be treated by the method of the invention are preferably those described above. The invention also provides the use of a compound according to the invention, for the manufacture of a medicament for the treatment or prophylaxis of a condition mediated by an estrogen receptor. Clinical conditions mediated by an estrogen receptor that may be treated by the method of the invention are preferably those described above.
The amount of active ingredient which is required to achieve a therapeutic effect will, of course, vary with the particular compound, the route of administration, the subject under treatment, including the type, species, age, weight, sex, and medical condition of the subject and the renal and hepatic function of the subject, and the particular disorder or disease being treated, as well as its severity. An ordinarily skilled physician, veterinarian or clinician can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.
Oral dosages of the present invention, when used for the indicated effects, will range between about 0.01 mg per kg of body weight per day (mg/kg/day) to about 100 mg/kg/day, preferably 0.01 mg per kg of body weight per day (mg/kg/day) to 10 mg/kg/day, and most preferably 0.1 to 5.0 mg/kg/day, for adult humans. For oral administration, the compositions are preferably provided in the form of tablets or other forms of presentation provided in discrete units containing 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 5.0, 10.0, 15.0, 25.0, 50.0, 100, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated. A medicament typically contains from about 0.01 mg to about 500 mg of the active ingredient, preferably from about 1 mg to about 100 mg of active ingredient. Intravenously, the most preferred doses will range from about 0.1 to about 10 mg/kg/minute during a constant rate infusion. Advantageously, compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily. Furthermore, preferred compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using those forms of transdermal skin patches well known to those of ordinary skill in the art. To be administered in the form of a transdermal delivery system, the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen.
While it is possible for the active ingredient to be administered alone, it is preferable for it to be present in a pharmaceutical formulation or composition. Accordingly, the invention provides a pharmaceutical formulation comprising a compound according to the invention, and a pharmaceutically acceptable diluent, excipient or carrier (collectively referred to herein as "carrier" materials). Pharmaceutical compositions of the invention may take the form of a pharmaceutical formulation as described below. The pharmaceutical formulations according to the invention include those suitable for oral, parenteral (including subcutaneous, intradermal, intramuscular, intravenous [bolus or infusion], and intraarticular), inhalation (including fine particle dusts or mists which may be generated by means of various types of metered does pressurized aerosols), nebulizers or insufflators, rectal, intraperitoneal and topical (including dermal, buccal, sublingual, and intraocular) administration, although the most suitable route may depend upon, for example, the condition and disorder of the recipient.
The formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing the active ingredient into association with the carrier which constitutes one or more accessory ingredients. In general the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.
Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, pills or tablets each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid, for example as elixirs, tinctures, suspensions or syrups; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion. The active ingredient may also be presented as a bolus, electuary or paste.
A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free- flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, lubricating, surface active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein. The present compounds can, for example, be administered in a form suitable for immediate release or extended release. Immediate release or extended release can be achieved by the use of suitable pharmaceutical compositions comprising the present compounds, or, particularly in the case of extended release, by the use of devices such as subcutaneous implants or osmotic pumps. The present compounds can also be administered liposomally.
Exemplary compositions for oral administration include suspensions which can contain, for example, microcrystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer, and sweeteners or flavoring agents such as those known in the art; and immediate release tablets which can contain, for example, microcrystalline cellulose, dicalcium phosphate, starch, magnesium stearate, calcium sulfate, sorbitol, glucose and/or lactose and/or other excipients, binders, extenders, disintegrants, diluents and lubricants such as those known in the art. Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes and the like. Disintegrators include without limitation starch, methylcellulose, agar, bentonite, xanthan gum and the like. The compounds of formula (I) can also be delivered through the oral cavity by sublingual and/or buccal administration. Molded tablets, compressed tablets or freeze- dried tablets are exemplary forms which may be used. Exemplary compositions include those formulating the present compound(s) with fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins. Also included in such formulations may be high molecular weight excipients such as celluloses (avicel) or polyethylene glycols (PEG). Such formulations can also include an excipient to aid mucosal adhesion such as hydroxy propyl cellulose (HPC), hydroxy propyl methyl cellulose (HPMC), sodium carboxy methyl cellulose (SCMC), maleic anhydride copolymer (e.g., Gantrez), and agents to control release such as polyacrylic copolymer (e.g. Carbopol 934). Lubricants, glidants, flavors, coloring agents and stabilizers may also be added for ease of fabrication and use. Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like. For oral administration in liquid form, the oral drug components can be combined with any oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like.
The compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, 1,2-dipalmitoylphosphatidylcholine, phosphatidyl ethanolamine (cephaline) , or phosphatidylcholine (lecithin).
Formulations for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilised) condition requiring only the addition of the sterile liquid carrier, for example saline or water-for-injection, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described. Exemplary compositions for parenteral administration include injectable solutions or suspensions which can contain, for example, suitable non-toxic, parenterally acceptable diluents or solvents, such as mannitol, 1,3- butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid, or Cremaphor. Exemplary compositions for nasal, aerosol or inhalation administration include solutions in saline, which can contain, for example, benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, and/or other solubilizing or dispersing agents such as those known in the art.
Formulations for rectal administration may be presented as a suppository with the usual carriers such as cocoa butter, synthetic glyceride esters or polyethylene glycol. Such carriers are typically solid at ordinary temperatures, but liquefy and/or dissolve in the rectal cavity to release the drug.
Formulations for topical administration in the mouth, for example buccally or sublingually, include lozenges comprising the active ingredient in a flavoured basis such as sucrose and acacia or tragacanth, and pastilles comprising the active ingredient in a basis such as gelatin and glycerine or sucrose and acacia. Exemplary compositions for topical administration include a topical carrier such as Plastibase (mineral oil gelled with polyethylene).
Preferred unit dosage formulations are those containing an effective dose, as hereinbefore recited, or an appropriate fraction thereof, of the active ingredient.
It should be understood that in addition to the ingredients particularly mentioned above, the formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents.
Whilst a compound of the invention may be used as the sole active ingredient in a medicament, it is also possible for the compound to be used in combination with one or more further active agents. Such further active agents may be further compounds according to the invention, or they may be different therapeutic agents, for example an antidepressant, an anxiolytic, an anti-psychotic, an agent useful in the prevention or treatment of osteoporosis, an agent useful in the prevention or treatment of cancer or other pharmaceutically active material. For example, the compounds of the instant invention may be effectively administered in combination with effective amounts of other agents such as an antidepressant, an anxiolytic, an anti-psychotic, an organic bisphosphonate or a cathepsin K inhibitor. In one preferred embodiment, the compounds of the invention may be effectively administered in combination with an effective amount of an antidepressant. Nonlimiting examples of antidepressants include noradrenaline reuptake inhibitors (NRI), selective serotonin reuptake inhibitors, monoamine oxidase inhibitors, tricyclic antidepressants (TCA), dopamine reuptake inhibitors (DRI), opioids, selective seretonic reuptake enhancers, tetracyclic antidepressants, reversible inhibitors of monoamine oxidase, melatonin agonists, serotonin and noradrenaline reuptake inhibitors (SNRI), corticotropin releasing factor antagonists, α- adrenoreceptor antagonists, 5HTl α receptor agonists and antagonists, lithium and atypical anti- psychotics. Examples of antidepressants of the SSRI class include Fluoxetine and Sertraline; examples of antidepressants of the SNRI class Venlafaxine, Citalopram, Paroxetine, Escitalopram, Fluvoxamine; examples of antidepressants of the SNRI class include Duloxetine; examples of antidepressants of the DRI and NRI classes include Bupropion; examples of antidepressants of the TCA class include Amitriptyline and Dothiepin (Dosulepin). Examples of atypical antipsychotics include: Clozapine,
Olanzapine, Risperidone, Quetiapine, Ziprasidone and Dopamine partial agonists. Nonlimiting examples of anxiolytics include benzodiazepines and non-benzodiazapines. Examples of benzodiazepines include lorazepam, alprazolam, and diazepam. Examples of non-benzodiazapines include Buspirone (Buspar®), barbiturates and meprobamate. One or more of those further anti-depressants may be used in combination.
Examples of anti-cancer agents include tamoxifene or an aromatase inhibitor, used in treatment of breast cancer.
In the event that hot flashes are induced by a particular treatment, a compound of the invention may be used in combination therapy with the agent of such treatment. Nonlimiting examples of such combination treatment therapies include: a compound of the invention in combination with tamoxifene treatment of breast cancer, a compound of the invention in combination with aromatase inhibitor treatment of breast cancer or a compound of the invention in combination with raloxifene treatment of osteoporosis.
Nonlimiting examples of above-mentioned organic bisphosphonates include adendronate, clodronate, etidronate, ibandronate, incadronate, minodronate, neridronate, risedronate, piridronate, pamidronate, tiludronate, zoledronate, pharmaceutically acceptable salts or esters thereof, and mixtures thereof. Preferred organic biphosphonates include alendronate and pharmaceutically acceptable salts and mixtures thereof. Most preferred is alendronate monosodium trihydrate.
The precise dosage of the bisphosphonate will vary with the dosing schedule, the oral potency of the particular bisphosphonate chosen, the age, size, sex and condition of the mammal or human, the nature and severity of the disorder to be treated, and other relevant medical and physical factors. Thus, a precise pharmaceutically effective amount cannot be specified in advance and can be readily determined by the caregiver or clinician. An appropriate amount can be determined by routine experimentation from animal models and human clinical studies. Generally, an appropriate amount of bisphosphonate is chosen to obtain a bone resorption inhibiting effect, i.e. a bone resorption inhibiting amount of the bisphonsphonate is administered. For humans, an effective oral dose of bisphosphonate is typically from about 1.5 to about 6000 μg/kg of body weight and preferably about 10 to about 2000 μg/kg of body weight. For human oral compositions comprising alendronate, pharmaceutically acceptable salts thereof, or pharmaceutically acceptable derivatives thereof, a unit dosage typically comprises from about 8.75 mg to about 140 mg of the alendronate compound, on an alendronic acid active weight basis, i.e. on the basis of the corresponding acid.
The compounds of the present invention can be used in combination with other agents useful for treating estrogen-mediated conditions. The individual components of such combinations can be administered separately at different times during the course of therapy or concurrently in divided or single combination forms. The present invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment and the term "administering" is to be interpreted accordingly. It will be understood that the scope of combinations of the compounds of this invention with other agents useful for treating estrogen-mediated conditions includes in principle any combination with any pharmaceutical composition useful for treating disorders related to estrogen functioning.
The above other therapeutic agents, when employed in combination with the compounds of the present invention, may be used, for example, in those amounts indicated in the Physicians' Desk Reference (PDR) or as otherwise determined by one of ordinary skill in the art.
Where the compounds of the invention are utilized in combination with one or more other therapeutic agent(s), either concurrently or sequentially, the following combination ratios and dosage ranges are preferred:
When combined with an antidepressant, an anxiolytic, an anti-psychotic, an organic bisphosphonate or a cathepsin K inhibitor, the compounds of formula (I) may be employed in a weight ratio to the additional agent within the range from about 10:1 to about 1 :10.
The compounds of the invention as described above also find use, optionally in labelled form, as a diagnostic agent for the diagnosis of conditions associated with malfunction of the estrogen receptor. For example, such a compound may be radioactively labelled.
The compounds of the invention as described above, optionally in labelled form, also find use as a reference compound in methods of discovering other agonists, partial agonists, antagonists or partial antagonists of the estrogen receptor. Thus, the invention provides a method of discovering a ligand of the estrogen receptor which comprises use of a compound of the invention or a compound of the invention in labelled form, as a reference compound. For example, such a method may involve a competitive binding experiment in which binding of a compound of the invention to the estrogen receptor is reduced by the presence of a further compound which has estrogen receptor-binding characteristics, for example stronger estrogen receptor-binding characteristics than the compound of the invention in question.
Numerous synthetic routes to the compounds of the present invention can be devised by any person skilled in the art and the possible synthetic routes described below do not limit the invention. Many methods exist in the literature for the synthesis of indoles, for example: Indoles Part One, W. J. Houlihan (ed.), 1972; Indoles, Sundberg, R. J., 1996; Heterocyclic Chemistry, Joule, J. A.; Mills, K. 2000; Chem. Rev., 2005, 105, 2873-2920; Org. Lett. 2006, 8, 5919-5922; and Bioorg. Med. Chem. Lett., 2007, 77, 902- 906. A number of possible synthetic routes are shown schematically below. Where appropriate, any initially produced compound according to the invention can be converted into another compound according to the invention by known methods.
General Method 1
Figure imgf000021_0001
(a) Aryliodide, Potassium phosphate, N,N'-dimethylethylenediamine, CuI, Toluene;
(b) t-BuLi, 1 ,2-dibromotetrachloroethane, THF; (c) BBr3, DCM
General Method 1 as shown in the reaction scheme above was used for the synthesis of the following Examples:
• Examples 46-159 and 211 - full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 46-159 and 211 are described in Examples 1-5, 16 and 38-40. • Examples 160-162 and 196-210 - full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 160-162 and 196-210 are described in Examples 1-4, 8, 16 and 38-40.
• Examples 169 and 173-195 - full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 169 and 173-195 are described in Examples 1-4, 8, 10-13, 14-16, 20, 25, 38-40 and 45.
• Examples 233-270 - full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 233-270 are described in Examples 1-4, 10-11, 14-16, 38-40 and 45. General Method 2
Figure imgf000022_0001
(a) NaH, R1-alkylester, THF; (b) Aniline, AcOH; (c) PIFA, CH2CI2, (d) BBr3, CH2CI2.
General Method 2 as shown in the reaction scheme above was used for the synthesis of Example 6.
General Method 3
Figure imgf000023_0001
(a) Aryliodide, Potassium phosphate, N,N'-dimethylethylenediamin, CuI, Toluene;
(b) Chlorosulphonyl isocyanate, 1 ,2-dichloorethane, THF; (c) Phosphorus oxychloride; (d) t-BuLi, 1 ,2-dibromotetrachloroethane, THF; (e) BF3SMe2, DCM
General Method 3 as shown in the reaction scheme above was used for the synthesis of Examples 212-232 and 271. Full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 212-232 and 271 are described in Examples 2-4, 8, 16, 24 and 38-40. General Method 4
Figure imgf000024_0001
(a) 2-(methylthio)isoindoline-1,3-dione, MgBr, DMA; (b) NBS, DMF; (C) R-iboronic acid, Pd(PPh3)4, NaI, NaCO3, DME, H2O (d) 2-Mercaptobenzoic acid, TFA; (e) NBS, DMF
General Method 4 as shown in the reaction scheme above was used for the synthesis of Examples 163-168 and 170-172. Full experimental details of the individual steps of the general method applicable for the synthesis of the final compounds of Examples 163-168 and 170-172 are described in Examples 2-4, 9, 20-21.
General Method 5
Figure imgf000024_0002
General Method 5 as shown in the reaction scheme above is also potentially applicable for synthesising the compounds of the invention. An appropriate 3-cyano-N-arylindole could be obtained from an N-arylindole via formylation as shown in step (a), for example by means of a Vilsmeier-Haack reaction, followed by reaction of the aldehyde with hydroxylamine hydrochloride and subsequent elimination using acetic anhydride as shown in step (b). The following Examples illustrate the invention. Example 1 2-Bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (El)
Scheme 1
Figure imgf000025_0001
(a) 4-lodoanisole, Potassium phosphate, N,N'-dimethylethylenediamine, CuI, Toluene;
(b) t-BuLi, 1 ,2-dibromotetrachloroethane, THF; (c) BBr3, DCM
Step (a): 1 eq 3-Cyanoindole, 2 eq 4-iodoanisole, 2.1 eq potassium phosphate, 4.5 eq N,N'-dimethylethylenediamine and 0.2 eq copper(I) iodide were mixed in an oven-dried vial and toluene was added. The mixture was stirred under N2-atmosphere at 110 0C over night. The reaction mixture was cooled to rt, filtered and evaporated in vacuo. The crude product was purified on silica using n-heptane: EtOAc (4:1) as mobile phase.
Step (b): l-(4-Methoxy-phenyl)-lH-indole-3-carbonitrile was dissolved in dry THF and cooled to -78 0C, 1.1 eq MBuLi was added drop wise and the mixture was stirred for one hour. A solution of 1.3 eq 1 ,2-dibromotetrachloroethane in dry THF was added and the mixture was stirred for 4 hours while slowly warming it up to rt and then quenched by addition Of H2O. The reaction mixture was diluted with DCM, phases were separated and the organic phase was evaporated in vacuo. The crude product was purified on silica using n-heptane: DCM (1:1) as mobile phase.
Step (c): 2-Bromo-l-(4-methoxy-phenyl)-lH-indole-3-carbonitrile was dissolved in dry DCM and cooled to 0 0C. 5 eq BBr3 (1.0 M solution in hexane) was added and the mixture was stirred over night. Still at 0 0C, the reaction was quenched with the addition of MeOH. The mixture was diluted with H2O and the phases were partitioned. The organic phase was concentrated and the crude product purified on silica using n-heptane: EtOAc (4:1) as mobile phase. ES/MS m/z: 313, 315 (M+H), 311, 313 (M-H); 1H NMR (acetone-d6, 500MHz): 7.69 (m, IH), 7.38-7.30 (m, 4H) and 7.16-7.10 (m, 3H).
Example 2 l-(4-Hydroxy-phenyl)-2-thiophen-3-yl-lH-indole-3-carbonitrile (E2) Scheme 2
Figure imgf000026_0001
To 2-bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (Example 1) was added 2 eq 3-thiopheneboronic acid, 2.1 eq potassium carbonate and 10 mol % tetrakis(triphenylphosphine) palladium. THF: EtOH: H2O (4:1:0.5) was added and the vial was flushed with nitrogen, sealed and stirred at 100 0C for 48 hours. The reaction mixture was cooled to rt, diluted with H2O, extracted with EtOAc and filtered through silica. The organic phase was evaporated to dryness and the crude product was subjected to reversed phase preparative HPLC. Appropriate fractions were combined, evaporated, and identified by 1H-NMR and LCMS. Purity was determined by analytical HPLC. ES/MS m/z: 317.9 (M+H), 314.8 (M-H); 1H NMR (acetone-d6, 500MHz): 7.73 (m, IH), 7.62 (dd, IH, J=1.3, 2.9Hz), 7.54 (dd, IH, J=2.9, 5.0Hz), 7.37-7.30 (m, 2H), 7.26 (m, 2H), 7.18 (m, IH), 7.15 (dd, IH, J=1.3, 5.0Hz) and 7.03 (m, 2H).
Example 3 2-(3-Cyano-furan-2-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (E3)
Scheme 3
Figure imgf000026_0002
2-Bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (Example 1, 40 mg, 0.13 mmol, 1 eq), 2-tributylstannanyl-furan-3-carbonitrile (63.5 mg, 0.17 mmol, 1.3 eq) and dichlorobis(triphenylphosphine)palladium(II) (9 mg, 0.01 mmol, 0.1 eq) were weighed into a microwave vial. Dioxane (1 ml) was added, the vial was flushed with nitrogen and capped. The reaction mixture was irradiated at 130 degrees for 30 min in the microwave and then the solution was filtered. Saturated aqueous ammonium chloride solution (5 ml) was added and the mixture was extracted several times with DCM. The combined DCM phases were passed through a phase separation membrane and evaporated. The crude product was purified by a quick silica gel flash chromatography, using n-heptane: ethyl acetate (7:3) gradient, to remove the remaining tin reagent rests. Appropriate fractions were collected and evaporated, dissolved in acetonitrile (1 ml), and purified by preparative HPLC to give 33 mg (79% yield) 99% pure product, as determined by analytical HPLC. (Column: Reprosil Pur 120 ODS-3 (Cl 8), 30x100 mm, 5μm. Mobile phase A: Formic acid 0.05%, Mobile phase B: ACN. Gradient 20% A-100% B. ES/MS m/z: 326.1 (M+H), 324.1 (M-H); 1HNMR (acetone-d6, 500MHz): 7.89 (d, IH, J=2.2Hz), 7.85 (m, IH), 7.48-7.43 (m, 2H), 7.34 (m, IH), 7.32 (m, 2H) and 7.04-7.01 (m, 3H).
Example 4 l-(4-Hydroxy-phenyI)-2-pyrrol-l-yl-lH-indole-3-carbonitrile (E4)
Scheme 4
Pyrrole, Cs2CO3, CuI, DMF
Figure imgf000027_0002
Figure imgf000027_0001
2-Bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (Example 1), 1.4 eq pyrrole, 2 eq cesium carbonate and 20 mol% copper(I) iodide was mixed in a oven-dried vial, DMF was added and the mixture was flushed with nitrogen. The vial was sealed and stirred at 120 °C for 48 hours. The reaction-mixture was cooled to rt, diluted with EtOAc and filtered through silica. The crude mixture was evaporated to dryness and subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by 1H-NMR and LC/MS. Purity was determined by analytical HPLC. ES/MS m/z: 300.2 (M+H), 298.3 (M-H); 1H NMR (acetone-d6, 500MHz): 7.74 (m, IH), 7.41-7.35 (m, 2H), 7.27 (m, 2H), 7.23 (m, IH), 6.98 (m, 2H), 6.95 (t, 2H, J=2.2Hz) and 6.23 (t, 2H, J=2.2Hz).
Example 5
2-Dimethylamino-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (E5) Scheme 5 Dimethylamine, DMF
Figure imgf000028_0001
Figure imgf000028_0002
2-Bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (Example 1) was mixed with excess dimethylamine in DMF (1:3) and the mixture was stirred in a sealed vial at 80 0C over night. The reaction-mixture was cooled to rt, diluted with H2O and DCM and then the phases were separated. The organic phase was evaporated to dryness and subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by 1H-NMR and LC/MS. Purity was determined by analytical HPLC. ES/MS m/z: 278.1 (M+H), 276.1 (M-H); 1H NMR (acetone-d6, 500MHz): 7.42 (m, IH), 7.33 (m, 2H), 7.17 (m, IH), 7.09-7.05 (m, 3H), 6.95 (m, IH) and 2.93 (s, 6H).
Example 6 l-(4-Hydroxy-phenyl)-2-isopropyl-lH-indole-3-carbonitrile (E6)
Scheme 6
Figure imgf000028_0003
(a) NaH, Ethyl isobutyrate, THF; (b) 4-Methoxyaniline, AcOH; (c) PIFA, CH2CI2; (d) BBr3, CH2CI2
Step (a): Benzylcyanide (1500mg, 12.8mmol) was dissolved in 150 ml dry THF and cooled down to 0 0C. The solution was stirred at that temperature while NaH (60% in mineral oil, 663mg) was added slowly. After the addition was completed, the mixture was stirred at 0 0C for 30 min and then the ice bath was removed and stirring continued at RT for 120 min. Ethyl isobutyrate (1785 mg, 15.4 mmol) was added at once and the reaction mixture heated at 60 0C for 2h. The THF was removed in vacuo and the residue was then poured into ice-water. HCl 6M was added with stirring until neutral pH was reached. The mixture was extracted with EtOAc, dried over MgSθ4 and concentrated in vacuo. The compound was purified by flash chromatography [silica; n-heptane-EtOAc (9:1)] to afford 4-methyl-3-oxo-2-phenyl- pentanenitrile (460 mg, 19%). Step (b): 4-Methyl-3-oxo-2 -phenyl -pentanenitrile (lOOmg, 0.53mmol) and 4-methoxyaniline were dissolved in AcOH 100% (ImI). The mixture was heated at 160 0C during 30min in the microwave. The solvent was co-evaporated with toluene in vacuo. The residue dissolved in CH2CI2 and filtered through a silica plug. The solvent was removed and the mixture obtained was used directly in the next step without further purification.
Step (c): The mixture obtained from step (b) (65mg) and PDPA (26mg) were dissolved in CH2Cl2 (dry, 1.5ml) and stirred at RT overnight. The mixture was extracted in NaHCθ3 / CH2Cl2 using a phase separator. The mixture was purified by preparative HPLC to afford the desired 2-isopropyl-l-(4-methoxy- phenyl)- 1 H-indole-3 -carbonitrile (3mg) .
Step (d): 2-Isopropyl-l-(4-methoxy-phenyl)-l H-indole-3 -carbonitrile (3mg, 0.01 mmol) was dissolved in dry CH2Cl2 (0.5 ml) and stirred at 0 0C. BBr3 (IM in CH2Cl2, 50μl) was added and the mixture left in the fridge with stirring overnight. Some drops of MeOH were added and stirred. The solvent was removed in vacuo and the mixture partitioned in H2O/ DCM. The organic phase was separated using a phase separator. The solvent was removed in vacuo and the mixture was chromatographed using a prepacked silica column (solvent: EtOAc:n-Heptane 3-7) to afford the desired l-(4-hydroxy-phenyl)-2-isopropyl- lH-indole-3-carbonitrile (1.1 mg, 39%). 1H NMR (acetone-d6, 500MHz): 7.64 (m, IH), 7.32 (m, 2H), 7.28 (m, IH), 7.22 (m, IH), 7.11 (m, 2H), 6.98 (m, IH), 3.07 (m, IH) and 1.43 (d, 6H, J=7.0Hz).
Example 7
2-Acetyl-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (E7)
The title compound was synthesized by hydrolysis of 2-(l-Ethoxy-vinyl)-l-(4-hydroxy-phenyl)-lH- indole-3 -carbonitrile. ES/MS m/z: 277.1 (M+H), 275.1 (M-H); 1H NMR (acetone-d6, 500MHz): 7.84 (d,
IH, J=7.5Hz), 7.49-7.42 (m, 2H), 7.32 (m, 2H), 7.18 (m, IH, J=8.0Hz), 7.06 (m, IH) and 2.46 (s, 3H).
Example 8 2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid (E8)
Scheme 7
Figure imgf000029_0001
2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (Example 51) was dissolved in a 1 :2 mixture of THF and LiOH (aq, 2 M) and run in a microwave at 160 0C for one hour. The mixture was diluted with H2O and DCM, acidified to pH 1 with HCl (1 M) and the phases separated. The organic phase was concentrated in vacuo and purified with Chromatotrone using 5 % MeOH in DCM as mobile phase. ES/MS m/z: 349.4 (M+H), 347.2 (M-H); 1H NMR (acetone-d6; 500MHz): 8.31 (m, IH), 7.32-7.23 (m, 3H), 7.20 (m, IH), 7.12 (br s, IH), 6.97 (m, 2H), 2.16 (s, 3H) and 2.05 (s, 3H).
Example 9 1- [l-(4-Hydroxy-phenyl)-2-phenyl-lH-indol-3-yl]-ethanone (E9)
Figure imgf000030_0001
(a) Ethyleneglycol monovinylether, Pd(OAc)2, dppp, KOAc, tBuNH3Br, toluene/H2O 1 :1 ;
(b) 3M HCI; (c) BBr3, DCM
Steps (a) and (b): 1 eq. 3-Bromo-l -(4-methoxy-phenyl)-2-phenyl-lH-indole (synthesised from l-(4- methoxyphenyl)-lH-indole by a method analogous to that used in steps (a), (b) and (c) of Example 21 followed by steps (a) and (b) of Example 22), 5 eq. ethylene glycol monovinyl ether, 5 mol% Pd(OAc)2, 10 mol% dppp, 1.3 eq. potassium acetate, 5 mol% tetrabutylammonium bromide were mixed with toluene/water in a microwave vial under nitrogen. The reaction was run in a microwave reactor at 150 0C for 20 min. 2 ml 3M HCl was added and the mixture was stirred at RT for 30 min. Water and DCM were added and the phases were separated. After evaporation of the solvents, the residue was purified by flash chromatography with heptane/EtOAc 9: 1.
Step (c): The starting material was dissolved in dry DCM under nitrogen and was cooled to 0 0C. BBr1 was added and the temperature was allowed to reach RT for 2h. The reaction was quenched with water and the phases were separated. After evaporation of the solvents, the residue was purified by preparative HPLC. ES/MS m/z: 328.16 (M+H), 326.2 (M-H); 1H NMR (DMSO-d6, 500MHz): 8.35 (m, IH), 7.43 (m, 2H), 7.40-7.37 (m, 3H), 7.28 (m, IH), 7.24 (m, IH), 7.10 (m, 2H), 7.00 (m, IH), 6.73 (m, 2H) and 1.88 (s, 3H). Example 10 l-(4-Hydroxy-phenyl)-2-phenyl-lH-indole-3-carboxylic acid amide (ElO)
Scheme 9
Figure imgf000031_0001
l-(4-Hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile (Example 46, 10 mg, 0.03 mmol) was dissolved in 1 ml of MeOH. H2O2 (1 ml, 35% by weight in H2O) and 2M KOH (0.5 ml) were added and the reaction mixture was heated to reflux over night. The mixture was acidified with IM HCl and EtOAc was added. The phases were separated and the organic solvents were evaporated. The crude product was purified by reversed phase preparative HPLC to give the title amide. ES/MS m/z: 329.1 (M+H), 327.13 (M-H); 1H NMR (acetone-d6, 500MHz): 8.34 (m, IH), 7.46 (m, 2H), 7.40-7.37 (m, 3H), 7.24-7.18 (m, 2H), 7.12 (m, 2H), 7.07 (m, IH) and 6.85 (m, 2H).
Example 11 (Z)-2-(3,5-dimethylisoxazol-4-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide
(Ell)
Scheme 10
Figure imgf000031_0002
Under nitrogen atmosphere, a solution of 2-(3,5-dimemyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH- indole-3-carbonitrile (Example 51), 12 eq hydroxylamine hydrochloride and 12 eq triethylamine in EtOH was heated at 100 0C for 24 hours. The reaction mixture was cooled to rt, diluted with methanol, precipitation filtered off and subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by 1H-NMR and LC/MS. Purity was determined by analytical HPLC. ES/MS nVz: 363.5 (M+H), 361.6 (M-H); 1H NMR (acetone-d6, 500MHz): 8.14-8.13 (s, OH), 8.08-8.04 (m, IH), 7.23-7.14 (m, 3H), 7.15-7.06 (m, 2H), 6.96-6.91 (m, 2H), 5.18-5.07 (m, 2H), 2.16 (s, 3H), 2.00 (s, 3H).
Examples 12 and 13
[2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indol-3-yl]-carbamic acid tert-butyl ester
(E12)
4-(3-Amino-2-(3,5-dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol (E13)
Scheme 11
Figure imgf000032_0001
(a) Diphenylphosphoryl azide, Et3N, tBuOH; (b) TFA, DCM
Step (a): A mixture of 2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-carboxylic acid(Example 8), diphenylphosphoryl azide (1.1 eq) and triethyl amine (1.1 eq) in tert-BuOH was heated in microwave reactor at 90 0C for 1 hour. After cooling, the mixture was subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, to provide [2-(3,5-dimethyl- isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indol-3-yl]-carbamic acid tert-buty] ester (E12). ES/MS m/z: 420.21 (M+H), 418.22 (M-H); 1H NMR (acetone-d6, 500MHz): 7.61 (m, IH), 7.26-7.13 (m, 5H), 6.93 (m, 2H), 2.22 (s, 3H), 1.89 (s, 3H) and 1.44 (s, 9H).
Step (b): At rt, tert-butyl 2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-ylcarbamate was dissolved in DCM and treated with catalytic amount of TFA until the reaction was complete. The mixture was concentrated in vacuo and co-evaporated with MeOH. 4-(3-Amino-2-(3,5-dimethylisoxazol- 4-yl)-lH-indol-l-yl)phenol (El 3) was identified with LC/MS and purity determined by analytical HPLC. ES/MS m/z: 320.2 (M+H) and 31 8.2 (M-H).
Example 14
(Z)-2-(3,5-Dimethylisoxazol-4-yl)-7-fluoro-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3- carboximidamide (E14) Scheme 12
Figure imgf000033_0001
(a) HCI (gas), Dioxane, MeOH; (b) Hydroxylamine hydrochloride, NaHCO3, EtOH
Step (a): HCl gas was bubbled into a cooled (O0C) solution of 2-(3,5-dimethylisoxazol-4-yl)-7-fluoro-l- (4-hydroxyphenyl)-lH-indole-3-carbonitrile (Example 230, 19 mg, 0.05 mmol) in dioxane (1.5ml) and MeOH (0.5 ml) for 10 min. The tube was sealed, the temperature was allowed to warm to RT and the mixture was stirred over night. The solvents were evaporated in vacuo.
Step (b): To a stirring solution of hydroxylamine hydrochloride (19 mg, 0.27 mmol) in water, was added solid NaHCO3 (23 mg, 0.27 mmol) at RT. The above amidate, dissolved in EtOH (1.5 ml), was added, and the solution was stirred at 9O0C in a sealed vial for Ih. The mixture was purified by preparative HPLC to provide the title compound (Z)-2-(3,5-dimethylisoxazol-4-yl)-7-fluoro-N'-hydroxy-l-(4- hydroxyphenyl)-lH-indole-3-carboximidamide in 10% yield. ES/MS m/z: 381.1 (M+H), 279.2 (M-H); 1H NMR (methanol-d4, 500MHz): 7.68 (dd, IH, J=8.1, 0.7Hz), 7.13 (m, IH), 7.08 (m, 2H), 6.93 (m, IH), 6.77 (m, 2H), 2.16 (s, 3H) and 2.00 (s, 3H).
Example 15 (Z)-2-(5-chlorothiophen-2-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indoIe-3-carboximidamide
(E15) Scheme 13
Figure imgf000033_0002
The compound 2-(5-chlorothiophen-2-yl)-l-(4-hydroxyphenyl)-lH-indole-3-carbonitrile (Example 86) was dissolved in DMSO (0.25 mL) and hydroxylamine (0.25 mL, 100 eq) was added from a 2M stock solution of hydroxylainine*hydrochloride neutralized with sodium hydroxide (pH = 7.01 as measured with pH-meter). The mixture was stirred at 60 0C for 18 hours. The cooled mixture was diluted with brine and extracted with EtOAc and subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by 1H-NMR and LC/MS. Purity was determined by analytical HPLC. ES/MS m/z: 386.3 (M+H), 384.2(M-H); 1H NMR (acetone-d6, 500MHz): 7.89 (m, IH), 7.21-7.13 (m, 4H), 7.04 (m, IH), 7.00-6.97 (m, 3H) and 6.91 (d, IH, J=4.0Hz).
Example 16 l-(2,3-Difluoro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-lII-indole-3-carbonitrile (E 16)
Scheme 14
Figure imgf000034_0001
To 2-bromo-l-(2,3-difluoro-4-hydroxyphenyl)-lH-indole-3-carbonitrile (Example 103) was added 1.5 eq 3,5-dimethylisooxazole-4-boronic acid, 4 eq potassium carbonate, 2 eq sodium iodide and 10 mol% tetrakis(triphenylphosphine)palladium. DME:H2O (1 :1) was added and the vial was flushed with nitrogen, sealed and stirred at 150 0C for 10 min. The reaction mixture was cooled to it, diluted with H2O and extracted with DCM. The organic phase was evaporated to dryness and purified on silica column using 1:1 n-heptane:EtOAc as mobile phase. ES/MS m/z: 366.20 (M+H), 364.20 (M-H); 1H NMR (CDCl3, 500MHz): 7.84 (d, IH), 7.39 (m, 2H), 7.19 (d, IH), 6.93 (broad m, 2H), 2.42 (s, 1.5H), 2.24 (s, 1.5 H), 2.19 (s, 1.5H), 2.01 (s, 1.5H)
Example 17 2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3 carbohydrazonamide (E 17)
Scheme 15
Figure imgf000035_0001
2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-carbimidate (Example 184) was dissolved in EtOH and 10 eq hydrazine was added. The resulting mixture was stirred at 90 0C for 10 hours. The reaction mixture was diluted with EtOAc and washed with brine. The organic phase was evaporated to dryness in vacuo and then purified using reversed phase preparative HPLC. Fractions were combined, concentrated and the final product was identified by 1H-NMR and LC/MS. Purity was determined by analytical HPLC. ES/MS m/z: 362.21 (M+H), 360.28 (M-H); 1H NMR (methanol-d3, 500MHz): 7.81 (m, IH), 7.36-7.32 (m, 3H), 7.08 (m, 2H), 6.90 (m, 2H), 2.16 (s, 3H) and 1.96 (s, 3H).
Example 18 4-(2-(3,5-Dimethylisoxazol-4-yl)-3-(l,2,4-oxadiazol-3-yl)-lH-indol-l-yl)phenol (E18)
Scheme 16
Figure imgf000035_0002
(Z)-2-(3,5-Dimethylisoxazol-4-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3 carboximidamide (Example 11) was dissolved in THF containing 4-A powdered molecular sieves. The mixture was stirred for 30 min under N2. NaH (60 % dispersion in mineral oil, 7.0 mg, 2.1 eq,) was added and the mixture was stirred at 60 0C for 20 min. After cooling to room temperature, ethyl formate (25.6 mg, 2.5 eq) in THF was added dropwise. The resulting mixture was heated at reflux for Ih and then cooled to room temperature. The mixture was filtered and concentrated. The residue was filtered through silica and then purified using reversed phase HPLC to give 4-(2-(3,5-dimethylisoxazol-4-yl)-3-(l,2,4-oxadiazol-3-yl)- lH-indol-l-yl)phenol as a white powder: ES/MS m/z: 395.17 (M+H), 393.19 (M-H); 1H NMR (acetone- d6, 500MHz): 9.21 (s, IH), 8.40 (m, IH), 7.37-7.31 (m, 2H), 7.26-7.22 (m, 3H), 6.98 (m, 2H), 2.17 (s, 3H) and 1.98 (s, 3H). Example 19
Methyl 2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-carboxylate (E 19)
Scheme 17
Figure imgf000036_0001
(a) Thionyl chloride, DMF; (b) Hydroxylamine, MeOH
Step (a): 2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-carboxylic acid (Example 8, 6.0 mg, 0.02 nimol) was dissolved in 0.5 ml of thionyl chloride and 2 drops of DMF were added. The mixture was heated at 700C for Ih and was then evaporated carefully in vacuo.
Step (b): 2-(3,5-Dimethylisoxazol-4-yl)-l -(4-hydroxyphenyl)-lH-indole-3-carbonyl chloride was dissolved in 0.5 ml of dry MeOH and the mixture was cooled on an ice-bath. Fresh hydroxylamine was prepared by pouring a MeOH solution (0.3 ml) of hydroxylamine hydrochloride (12 mg, 0.17 mmol) into a MeOH solution (0.3 ml) of KOH (19 mg, 0.33 mmol). The mixture was filtered through a syringe filter into the cooled acid chloride solution. After 5 min, the cooling bath was removed and the reaction was allowed to warm to RT and was stirred for 10 min. Water, IM HCl and EtOAc were added and the phases were separated. After evaporation of the solvents, the residue was purified by preparative HPLC to provide methyl 2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-carboxylate in 16% yield. ES/MS m/z: 363.11 (M+H), 361.13 (M-H); IH NMR (methanol-d4, 500MHz): 8.21 (m, IH), 7.32- 7.26 (m, 2H), 7.20 (m, IH), 7.13 (br s, IH), 7.01 (br s, IH), 6.88 (m, 2H), 3.83 (s, 3H), 2.12 (s, 3H) and 2.03 (s, 3H).
Example 20 2-(3,5-Dimethylisoxazol-4-yl)-N-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboxamide (E20)
Scheme 18
Figure imgf000037_0001
(a) Oxalyl dichloride, DMF, DCM; (b) Hydroxylamine, Et3N, NMP
Step (a): Oxalyl dichloride (75 μl, 0.86 mmol) and a drop of DMF were added to a cooled (00C) solution of 2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-carboxylic acid (Example 8, 30 mg, 0.09 mmol) in dry DCM. The temperature was allowed to warm to RT and stirred for 2h. The solvents were evaporated.
Step (b): A mixture of hydroxylamine hydrochloride (30 mg, 0.43 mmol) and triethylamine (60 μl, 0.43 mmol) in 3ml of NMP was added to the crude product from step (a). The mixture was stirred at RT for 15 min. Water and EtOAc were added and the phases were separated. After evaporation of the solvents, the residue was purified by preparative HPLC to provide the title compound 2-(3,5-dimethylisoxazol-4-yl)- N-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboxamide in 32% yield. ES/MS m/z: 364.15 (M+H), 362.19 (M-H); IH NMR (acetone-d6, 500MHz): 7.40 (m, IH), 6.80-6.70 (m, 3H), 6.62 (m, 2H), 6.39 (m, 2H), 1.65 (s, 3H) and 1.49 (s, 3H).
Example 21 4-[2-(3,5-Dimethyl-isoxazol-4-yl)-3-methanesulfonyl-indol-l-yl]-phenol (E21)
Scheme 19
Figure imgf000038_0001
(a) 2-(methylthio)isoindoline-1 ,3-dione, MgBr, DMA; (b) NBS, DMF;
(c) S.δ-dimethylisoxazole^-ylboronic acid, Pd(PPh3)4, NaI, NaCO3, DME, H2O
(d) Oxone, H2O, MeOH; (e) BBr3, DCM
Step (a): l-(4-methoxyphenyl)-lH-indole (synthesised from indole by an arylation process analogous to that described in step (a) of Example 1 [arylation process also described in J. Org.Chem. 2008, 73 (14), 5529-5535], 1.0 g, 4.48 mmol), 2-(methylthio)isoindoline-l,3-dione (0.95 g, 4.93 mmol) and magnesium bromide (8 mg, 0.045 mmol) were mixed in degassed DMA and stirred under an atmosphere of nitrogen at 900C for 90 min. IM NaOH and EtOAc were added. The phases were separated and the organic solvents were evaporated. The residue was purified by flash chromatography with heptane/EtAc 20: 1 to provide l-(4-methoxyphenyl)-3-(methylthio)-lH-indole in 80% yield. ES/MS m/z: 270.11 (M+H).
Step (b): NBS (529 mg, 2.97 mmol) was added to a cooled (00C) solution of l-(4-methoxyphenyl)-3- (methylthio)-lH-indole (800 mg, 2.97 mmol) in 10 ml of DMF. The temperature was allowed to warm to RT and the mixture was stirred at RT for 30 min. Water and DCM were added and the phases were separated. After evaporation of the solvents, the residue was purified by flash chromatography with heptane/EtAc 20:1 to provide 2-bromo-l-(4-methoxyphenyl)-3-(methylthio)-lH-indole in 66% yield. ES/MS m/z: 348.04, 350.01 (M+H).
Step (c): 4-(l-(4-methoxyphenyl)-3-(methylthio)-lH-indol-2-yl)-3,5-dimethylisoxazole was synthesized from the product of step (b) using a procedure analogous to that described in Example 16.
Step (d): Oxone (152 mg, 0.25 mmol) was mixed with water (1 ml) and added to a cooled (O0C) mixture of 4-(l-(4-methoxyphenyl)-3-(methylthio)-lH-indol-2-yl)-3,5-dimethylisoxazole (30 mg, 0.08 mmol) in 2 ml of MeOH. The temperature was allowed to warm to RT and the slurry was stirred at RT over night. IM HCl and EtOAc were added and the phases were separated. The organic phase was concentrated.
Step (e): 4-(l-(4-Methoxyphenyl)-3-(methylsulfonyl)-lH-indol-2-yl)-3,5-dimethylisoxazole was dissolved in 2 ml of dry DCM and cooled to -78°C under nitrogen. BBr3 (31 μl, 0.33 mmol) was added and the temperature was allowed to warm to RT for 2h. Water and EtOAc were added and the phases were separated. After evaporation of the solvents, the residue was purified by preparative HPLC to provide 4-[2-(3,5-dimethyl-isoxazol-4-yl)-3-methanesulfonyl-indol-l-yl]-phenol in 27% yield. ES/MS m/z: 383.11 (M+H), 381.13 (M-H); IH NMR (acetone-d6, 500MHz): 8.12 (m, IH), 7.39-7.34 (m, 2H), 7.25-7.19 (m, 3H), 6.98 (m, 2H), 3.06 (s, 3H), 2.30 (s, 3H) and 2.09 (s, 3H).
Example 22 l-(2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2,2,2-trifluoroethanone (E22)
Scheme 20
Figure imgf000039_0001
(a) 2-Mercaptobenzoic acid, TFA; (b) NBS, DMF; (c) n-BuLi, 2,2,2- trifluoroacetic anhydride, THF; (d) BBr3, DCM
Step (a): 4-(l-(4-Methoxyphenyl)-3-(methylthio)-lH-indol-2-yl)-3,5-dimethylisoxazole (the intermediate product of step (c) from the synthesis of Example 21, 140 mg, 0.38 mmol) and 2-mercaptobenzoic acid (118 mg, 0.77 mmol) were added to 5 ml of trifluoroacetic acid at RT. The mixture was stirred as a slurry at RT under an atmosphere of nitrogen over night. 2M NaOH and EtAc were added and the phases were separated. The solvents were evaporated and the residue was purified by flash chromatography with heptane/EtAc 4:1 as eluent to provide 4-(l-(4-methoxyphenyl)-lH-indol-2-yl)-3,5-dimethylisoxazole in 86% yield. ES/MS m/z: 319.1 (M+H)
Step (b): NBS (59 mg, 0.33 mmol) was added to a cooled (00C) solution of 4-(l-(4-methoxyphenyl)-lH- indol-2-yl)-3,5-dimethylisoxazole (105 mg, 0.33 mmol) in 5 ml of DMF. The temperature was allowed to warm to RT and the mixture was stirred at RT for 30 min. DMF was evaporated. DCM and water were added and the phases were separated. After evaporation of the solvents, the residue was purified by flash chromatography with heptane/EtOAc 9:1 to provide 4-(3-bromo-l -(4-methoxyphenyl)-lH-indol-2-yl)- 3,5-dimethylisoxazole in 98% yield. ES/MS m/z: 365.14 (M+H), 363.30 (M-H).
Step (c): n-BuLi (10 μl, 0.03 mmol) was added to a cooled (-780C) solution of 4-(3-bromo-l-(4- methoxyphenyl)-lH-indol-2-yl)-3,5-dimethylisoxazole (10 mg, 0.03 mmol) under an atmosphere of nitrogen. After 5 min, 2,2,2-trifluoroacetic anhydride (7 μl, 0.05 mmol) was added. The temperature was allowed to warm to RT and the mixture was stirred over night. IM NaHCO3 and DCM were added, the phases were separated and the solvents were evaporated.
Step (d): 1 -(2-(3,5-dimethylisoxazol-4-yl)-l -(4-methoxyphenyl)-lH-indol-3-yl)-2,2,2-trifluoroethanone was dissolved in dry DCM and the mixture was cooled on an ice-bath under an atmosphere of nitrogen. BBr3 (17 μl, 0.1 mmol) was added and the temperature was allowed to warm to RT and the mixture was stirred over night. Water, DCM and some dioxane were added, the phases were separated and the solvents were evaporated. The residue was passed through a short plug of silica with EtOAc as eluent. The residue was purified by preparative HPLC to provide the title compound l-(2-(3,5-dimethylisoxazol-4-yl)-l-(4- hydroxyphenyl)-lH-indol-3-yl)-2,2,2-trifluoroethanone in 46% yield. ES/MS m/z: 401.1 (M+H), 399.1 (M-H); lH NMR (methanol-d4, 500MHz): 8.26 (m, IH), 7.41-7.34 (m, 2H), 7.18 (m, IH), 7.11 (m, 2H), 6.89 (m, 2H), 2.19 (s, 3H) and 2.01 (s, 3H).
Example 23 4-[3-bromo-2-(3,5-dimethylisoxazol-4-yl)-lH-indole-l-yl]phenol (E23) Scheme 21
Figure imgf000040_0001
At 00C, 10 eq of boron trifluoride-dimethyl sulfide was added to 4-[3-bromo-l-(4-methoxyphenyl)-lH- indol-2-yl]-3,5-dimethylisoxazole (the intermediate product of step (b) from the synthesis of Example 22), dissolved in DCM and stirred at ambient temperature over night. The mixture was diluted with EtOAc and washed with brine, the organic phase was concentrated and subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by ES/MS m/z: 385.1 (M+H), 383.09 (M-H) and 1HNMR (acetone-d6, 500MHz): 7.60 (m, IH), 7.31-7.23 (m, 3H), 7.17 (m, 2H), 6.95 (m, 2H), 2.28 (s, 3H) and 1.99 (s, 3H).
Example 24 2-bromo-4-fluoro-l-(4-hydroxyphenyl)-lH-indole-3-carbonitrile (E24)
Scheme 22
Figure imgf000041_0001
(a) Chlorosulfonyl isocyanate, 1 ,2-dichloroethane; (b) Phosphorus oxychloride; (c) t-BuLi, 1 ,2-dibromotetrachloroethane,THF; (d) BF3SMe2, DCM.
Step (a): 4-Fluoro-l-(4-methoxyphenyl)-lH-indole (synthesised from 4-fluoro-indole by an arylation process analogous to that described in step (a) of Example 1 [arylation process also described in J.
Org.Chem. 2008, 73 (14), 5529-5535]) was dissolved in 1,2-dichloroethane and 1.2 eq of chlorosulfonyl isocyanate was added, and the mixture was stirred at room temperature for 2 hours. To the mixture was added water and the pH adjusted to neutral by addition of NaOH (aq, IM), then the mixture was extracted with EtOAc. The organic phase was washed with brine and dried over Na2SO4. The crude product was purified on a Silica column using 50 % EtOAc in ^-heptane as mobile phase. Step (b): To 4-fluoro-l-(4-methoxyphenyl)-lH-indole-3-carboxamide was added 50 eq of phosphorus oxychloride and the reaction run neat at 60 0C for 2 hours. The mixture was cooled to rt and co-evaporated with toluene. The residue was diluted with EtOAc and washed first with sat. NaHCO3, then brine, and the organic phase dried over Na2SO4. The crude product was purified on a Silica column using 10 % EtOAc in ^-heptane as mobile phase.
Step (c): The intermediate was synthesized from the product of step (b) using a procedure analogous to that described in step (b) of Example 1.
Step (d): The title compound 2-bromo-4-fluoro-l-(4-hydroxyphenyl)-lH-indole-3-carbonitrile was synthesized from the product of step (c) using a procedure analogous to that described in Example 23. ES/MS m/z: 333.04 (M+H), 331.04 (M-H); 1H NMR (acetone-d6, 500MHz): 7.44 (dd, IH, J=8.9, 2.6Hz), 7.39 (m, 2H), 7.18 (dd, IH, J=8.5, 4.2Hz) and 7.15-7.09 (m, 3H).
Example 25
(Z)-2-(4-Fluorophenoxy)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide (E25) Scheme 23
Figure imgf000042_0001
(a) 4-Fluorophenol, potassium carbonate, DMF; (b) BF3SMe2, DCM; (c) 1.HCI (gas), Dioxane, MeOH 2. Hydroxylamine hydrochloride, NaHCO3, EtOH
Step (a): A mixture of 2-bromo-l -(4-methoxyphenyl)-lH-indole-3-carbonitrile (the intermediate product of step (b) from the synthesis of Example 1), 5 eq 4-fluorophenol and 5 eq potassium carbonate was heated at 200 0C for 20 minutes in a microwave reactor under inert athmosphere. The cooled mixture was diluted with water and extracted with EtOAc, the combined organic phases were dried over Na2SC>4 and concentrated. The crude product was purified on a Silica column using 20 % EtOAc in n-heptane as mobile phase.
Step (b): The compound 2-bromo-4-fluoro-l-(4-hydroxyphenyl)-lH-indole-3-carbonitrile was synthesized from the product of step (a) using a procedure analogous to that described in Example 23.
Step (c): The title compound (Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-phenoxy-lH-indole-3- carboximidamide was synthesized from the product of step (b) using procedures analogous to those described in Example 14. ES/MS m/z: 360.18 (M+H), 358.22 (M-H); 1H NMR (acetone-d6, 500MHz): 8.24 (m, IH), 7.25-7.14 (m, 6H), 7.09 (m, IH), 7.00 (m, IH) and 6.90-6.85 (m, 4H).
Example 26 4-(2-(3,5-Dimethylisoxazol-4-yl)-3-nitro-lH-indol-l-yl)phenol (E26) Scheme 24
Figure imgf000043_0001
(a) Nitric acid, HOAc, DCM; (b) BF3SMe2, DCM
Step (a): To a solution of 4-(l-(4-methoxyphenyl)-lH-indol-2-yl)-3,5-dimethylisoxazole (the intermediate product of step (a) from the synthesis of Example 22) in DCM was slowly added a mixture of 2 eq nitric acid and 20 eq acetic acid. After 1 hour stirring at rt, the mixture was diluted with water and the phases partitioned. The organic phase was concentrated and purified on a Silica column using 30 % EtOAc in n-heptane as mobile phase.
Step (b): The title compound 4-(2-(3,5-dimethylisoxazol-4-yl)-3-nitro-lH-indol-l-yl)phenol was synthesized from the product of step (a) using a procedure analogous to that described in Example 23. ES/MS m/z: 350.18 (M+H), 348.22 (M-H); 1H NMR (acetone-d6, 500MHz): 8.34 (m, IH), 7.50 (m, IH), 7.44-7.40 (m, 2H), 7.29 (m, IH), 7.20 (m, IH), 7.01 (m, 2H), 2.22 (s, 3H) and 2.11 (s, 3H). Example 27 4-(3-(Dihydroxyamino)-2-(3,5-dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol (E27)
Scheme 25
Figure imgf000044_0001
(a) Pd/C (10%), EtOH; (b) BF3SMe2, DCM
Step (a): 4-(l-(4-Methoxyphenyl)-3-nitro-lH-indol-2-yl)-3,5-dimethylisoxazole (the intermediate product of step (a) from the synthesis of Example 26) was dissolved in EtOH (99 %), a catalytic amount of Pd/C (10 %) was added and the mixture was stirred under 4 psi H2 over night. The mixture was filtered through a plug of Celite, concentrated and purified on a Silica column using 45 % EtOAc in rø-heptane as mobile phase.
Step (b): The title compound 4-(3-(dihydroxyamino)-2-(3,5-dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol was synthesized from the product of step (a) using a procedure analogous to that described in Example 23. ES/MS m/z: 351.13 (M+H), 350.18 (M-H); 1H NMR (acetone-dβ, 500MHz): 8.31 (d, IH, J=8.2Hz), 7.44 (t, IH, J=7.4Hz), 7.36 (m, IH), 7.30 (m, 2H), 7.22 (d, IH, J=8.2Hz), 7.05 (m, 2H), 1.81 (s, 3H) and 1.80 (s, 3H).
Example 28 N-(2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)acetamide (E28)
Figure imgf000044_0002
(a) NH4CI, Zn, DMF/H2O; (b) Acetyl chloride, NaHCO3, Et2O; (c) BF3SMe2, DCM Step (a): To a solution of 4-[l-(4-methyoxyphenyl)-3-nitro-lH-indol-2-yl]-3,5-dimethylisoxazole (the intermediate product of step (a) from the synthesis of Example 26) in DMF / water (10:1) at 0 0C, was added ammonium chloride (5 eq) and zinc (5 eq). The mixture was stirred for 2 hours at that temperature and then diluted with EtOAc, the formed precipitate was filtered off and the organic phase washed with brine and dried over Na2SO4. The mixture was concentrated and used without further purification in the next step.
Step (b): 2-(3,5-Dimethylisoxazol-4-yl)-l-(4-methoxyphenyl)-lΗ-indol-3-amine was dissolved in diethyl ether and cooled to 0 0C, then was added NaHCO3 (2 eq) and acetyl chloride (2 eq) as a solution in diethyl ether, the mixture was kept at O 0C and stirred for 1 hour. The solvent was removed and the residue dissolved in EtOAc and washed with water, then subsequently with brine, then dried over Na2SO4. The mixture was concentrated and used without further purification in the next step.
Step (c): The title compound N-(2 -(3, 5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3- yl)acetamide was synthesized from the product of step (b) using a procedure anaologous to that described in Example 23. ES/MS m/z: 361.21 (M+H), 360.17 (M-H); 1HNMR (acetone-d6, 500MHz): 7.58 (m, IH), 7.21-7.11 (m, 5H), 6.93 (m, 2H), 2.20 (s, 3H), 2.07 (s, 3H) and 1.88 (s, 3H).
Example 29 N-(2-(3,5-dimethyIisoxazoI-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)methanesulfonamide (E29)
Scheme 27
Figure imgf000045_0001
(a) Methanesulphonyl chloride, NaHCO3, Et2O; (b) BF3SMe2
Step (a): 2-(3,5-Dimethylisoxazol-4-yl)-l-(4-methoxyphenyl)-lH-indol-3-amine (the intermediate product of step (a) from the synthesis of Example 28) was dissolved in diethyl ether and cooled to 0 0C, then was added NaHCO3 (2 eq) and methanesulfonyl chloride (2 eq) as a solution in diethyl ether, the mixture was kept at 0 0C and stirred for 1 hour, then at rt over weekend. The solvent was removed and the residue dissolved in EtOAc and washed with water, then subsequently with brine, then dried over Na2SO4. The mixture was concentrated and used without further purification in the next step. Step (b): The title compound N-(2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3- yl)methanesulfonamide was synthesized from the product of step (a) using a procedure analogous to that described in Example 23. ES/MS m/z: 398.17 (M+H), 396.15 (M-H); 1H NMR (acetone-d6, 500MHz): 7.82 (m, IH), 7.24-7.20 (m, 3H), 7.17 (m, 2H), 6.95 (m, 2H), 2.89 (s, 3H), 2.26 (s, 3H) and 1.96 (s, 3H).
Example 30 l-(2-(3,5-DimethyIisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)urea (E30)
Scheme 28
Figure imgf000046_0001
(a) lsocyanato trimethylsilane, DMF; (b) HI
Step (a): A degassed (N2) mixture of 2-(3,5-dimethylisoxazol-4-yl)-l-(4-methoxyphenyl)-lH-mdol-3- amine (the intermediate product of step (a) from the synthesis of Example 28) and isocyanatotrimethylsilane (6 eq) in DMF was heated in a microwave reactor at 200 0C for 2 x 20 min. The mixture was cooled and concentrated and used without further purification in the next step.
Step (b): l-(2-(3,5-Dimethylisoxazol-4-yl)-l-(4-methoxyphenyl)-lH-indol-3-yl)urea was heated neat in hydriodic acid (aq, 57 %) at 100 0C for 3 hours. The reaction mixture was cooled and concentrated, and co-evaporated with DCM and EtOAc. The residue was subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by 1H NMR. ES/MS m/z: 363.19 (M+H), 362.18 (M-H); 1H NMR (acetone-dό, 500MHz): 7.65 (m, IH), 7.22-7.09 (m, 5H), 6.93 (m, 2H), 2.25 (s, 3H) and 1.94 (s, 3H).
Examples 31 and 32
4-(2-(3,5-Dimethylisoxazol-4-yl)-3-thiocyanato-lH-indol-l-yl)phenol (E31)
2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl N'-hydroxycarbamimidothioate
(E32)
Scheme 29
Figure imgf000047_0001
(a) NH4SCN, Mn(OAc)3, HOAc; (b) BBr3, DCM; (c) Hydroxylamine, NaHCO3 (aq), DMSO
Step (a): 4-(l-(4-Methoxyphenyl)-lH-indol-2-yl)-3,5-dimethylisoxazole (the intermediate product of step (a) from the synthesis of Example 22) was dissolved in AcOH and 1.2 eq ammonium thiocyanate was added. 3 eq Manganese (III) acetate was then added and the reaction was stirred at room temperature for 45 minutes after which monitoring on TLC showed full consumption of the starting material. Reaction mixture was diluted with EtOAc and washed with Brine. The phases were partitioned and the organic phase was washed with NaHCO3 (aq). Organic phase was then evaporated to dryness in vacuo and the crude product was used without further purification. ES/MS m/z: 376.14 (M+H);
Step (b): The product of step (a) was subjected to demethylation using BBr3 according to the process described in step (c) of Example 9 to give 4-(2-(3,5-dimethylisoxazol-4-yl)-3-thiocyanato-lH-indol-l- yl)phenol (E31). ES/MS m/z: 362.14 (M+H), 360.18 (M-H); 1H NMR (acetone-d6, 500MHz): 7.86 (m, IH), 7.43-7.37 (m, 2H), 7.31 (m, IH), 7.26 (m, 2H), 6.98 (m, 2H), 2.34 (s, 3H) and 2.03 (s, 3H).
Step (c): 4-(2-(3,5-Dimethylisoxazol-4-yl)-3-thiocyanato-lH-indol-l-yl)phenol was dissolved in DMSO and 10 eq 2 M hydroxylamine/NaHCO3 (aq) stock solution was added. The reaction was stirred at 65 0C overnight and then diluted with H2O. A precipitation was formed after stirring for 5 min. The mixture was diluted with EtOAc and diethylether, washed with NH4Cl (aq) and phases were partitioned. Organic phase was evaporated to dryness in vacuo and subjected to purification on reverse phase preparative HPLC to provide the title compound 2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl N'- hydroxycarbamimidothioate (E32). ES/MS m/z: 395.17 (M+H), 393.19 (M-H); 1H NMR (acetone-d6, 500MHz): 8.88 (broad s, IH), 8.73 (broad s, IH), 7.80 - 7.78 (m, IH), 7.29 - 7.24 (m, 3H), 7.18 (d, 2H), 6.95 (d, 2H), 5.11 (broad s, 2H), 2.28 (s, 3H), 2.00 (s, 3H)
Example 33 4-(3-benzyl-2-phenyl-lH-indol-l-yI)phenol (E33)
Scheme 30
Figure imgf000048_0001
(a) Benzaldehyde, TFA, Et3Si, DCM; (b) BBr3, DCM
Step (a): A mixture of 19μl TFA and 80μl Et3SiH in 0.5 ml dry DCM was added drop wise over 5 min. to a mixture of 47 mg l-(4-methoxyphenyl)-2-phenyl-lH-indole (synthesised from 2-phenylindole by an arylation process as described in J. Org.Chem. 2001, 66 (23), 7729-7737) and 19μl benzaldehyde in 1 ml dry DCM at O0C under nitrogen. The mixture was allowed to reach room temp over night after which the mixture was cooled to 00C and 160 μl Et3SiH and 38 μl TFA dissolved in 1 ml CH2Cl2 was added. The mixture was stirred for 40 min and then basifϊed with NaOH 2M (~pH 13) followed by addition of brine, extraction with dichloromethane (x3) and evaporation. 0.2 g Solid supported TsNHNH2 was added to the crude product together with 3 ml dichloromethane. The mixture was stirred gently for Ih 20 min. The polymer was filtered off and the crude product was filtered through a silica gel plug. The product was obtained pure without need for further purification.
Step (b): The product of step (a) was subjected to demethylation using BBr3 according to the process described in step (c) of Example 9 to give 4-(3-benzyl-2-phenyl-lH-indol-l-yl)ρhenol. ES/MS m/z:
376.4 (M+H), 374.2 (M-H); 1H NMR (acetone-d6, 500MHz): 7.86 (m, IH), 7.43-7.37 (m, 2H), 7.31 (m, IH), 7.26 (m, 2H), 6.98 (m, 2H), 2.34 (s, 3H) and 2.03 (s, 3H).
Examples 34 and 35 2-(2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2-oxoacetamide(E34)
2-(2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2-(hydroxyiniino)acetamide
(E35)
Scheme 31
Figure imgf000049_0001
(a) BBr3, DCM; (b) Oxalyl chloride.DCM, NH3/Me0H, (c) NH2OH, EtOH
Step (a): 4-(2-(3,5-Dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol was synthesized from 4-(l -(4- methoxyphenyl)-lH-indol-2-yl)-3,5-dimethylisoxazole (the intermediate product of step (a) from the synthesis of Example 22) via an analogous process to that described in step (c) of Example 1.
Step (b): 4-(2-(3,5-Dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol was dissolved in 1 ml dry dichloromethane under nitrogen and cooled to O0C. 4 ml Oxalyl chloride was added and the mixture was stirred at 00C for 2 h 15 min. and then at room temp for Ih 15 min. The mixture was concentrated under vacuum without heating and ImI NIVMeOH (sat.) added. The mixture was stirred for Ih and then concentrated. The crude product was purified by flash chromatography (EtO Ac/heptane; 4:6 to 8:2) to provide 2-(2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2-oxoacetamide (E34). ES/MS m/z: 376.18 (M+H), 374.17 (M-H); 1H NMR (acetone-d6, 500MHz): 8.33 (m, IH), 7.37-7.15 (m, 5H), 6.98 (br s, 2H), 2.27 (s, 3H) and 2.00 (s, 3H).
Step (c): 50 μl Pyridine was added to 8.8 mg 2-(2-(3,5-dimethylisoxazol-4-yl)-l -(4-hydroxyphenyl)-lH- indol-3-yl)-2-oxoacetamide and 32.6 mg hydroxylamine hydrochloride in 1.5 ml EtOH in a microwave vial. The vial was sealed and flushed with nitrogen. The mixture was heated to 1500C for 7.5 min. and then to 1500C for 5 min. in a Biotage Initiator microwave oven. The mixture was concentrated in vacuo and purified by preparative HPLC (formic acid buffer/acetonitrile). 2-(2-(3,5-dimethylisoxazol-4-yl)-l -
(4-hydroxyphenyl)-lH-indol-3-yl)-2(hydroxyimino)acetamide (E35) was obtained as a mixture of syn and anti oxime isomers. ES/MS m/z: 391.4 (M+H), 389.5 (M-H); 1H NMR (DMSO-d6, 500MHz): 7.37 (m, IH), 7.20-7.03 (m, 5H), 6.84 (m, 2H), 2.15 (s, 3H) and 1.77 (s, 3H).
Examples 36 and 37
2-(2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2-hydroxyacetamide (E36) 2-(2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)acetamide (E37)
Scheme 32
Figure imgf000050_0001
(a) NaBH4, EtOH; (b) Et3Si, TFA, DCM
Step (a): 9 mg 2-(2-(3,5-Dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2-oxoacetamide (Example 34) was mixed with 1 ml EtOH, 1.8 mg OfNaBH4 was added and the mixture was stirred for 1.5 h. The mixture was evaporated, water was added, followed by extraction with EtOAc. The crude product was purified by reversed phase flash chromatography (acetonitrile/water) to provide 2-(2-(3,5- dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2-hydroxyacetamide (E36). ES/MS m/z: 376.3 (M-H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.13-6.93 (m, 5H), 6.79 (m, 2H), 2.23, 2.21 (two s, 3H) and 1.93, 1.90 (two s, 3H).
Step (b): 1 ml TFA and 19 μl Et3SiH were added to 2-(2-(3,5-dimethylisoxazol-4-yl)-l-(4- hydroxyphenyl)-lH-indol-3-yl)-2-hydroxyacetamide at 00C and the mixture was stirred for 60 min. The solvent was evaporated without heating followed by short silica gel purification (EtO Ac/heptane; 1 :9 to 8:2) to provide 2-(2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)acetamide (E37). ES/MS m/z: 362.3 (M+H); 1H NMR (methanol-d3, 500MHz): 8.98 m, IH), 8.46-8.35 (m, 5H), 8.16 (m, 2H), 4.78 (d, 2H, J=I .9Hz), 3.54 (s, 3H) and 3.19 (s, 3H).
Example 38 2-((Z)-But-l-enyl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile (E38)
Scheme 33
Figure imgf000051_0001
(a) BuLi, tributyltin chloride, THF; (b) (Z)-i-bromobutene, dipalladium Tri-(1 E,4E)-1 ,5-diphenylpenta-1 ,4-dien-3-one, tri o-tolylphosphine, DMF;
(C) BF3SMe2, DCM
Step (a): To a cooled solution of 2-bronio-l-(4-methoxyphenyl)-lH-indole-3-carbonitrile (the intermediate product of step (b) from the synthesis of Example 1) in dry THF at -78 0C, was slowly added «-BuLi (1.6 M in hexane, 1.2 eq) and the mixture stirred for 30 min. Tributyltin chloride (1.5 eq) was added and the mixture kept at -78 0C for 1 hour, then stirred at rt over night. The mixture was quenched by addition of sat. NH4Cl and then concentrated. The residue was taken up in EtOAc, washed with brine, dried over Na2SO4 and purified on a Silica column using 20 % EtOAc in rø-heptane as mobile phase.
Step (b): A mixture of l-(4-methoxyphenyl)-2-(tributylstannyl)-lH-indole-3-carbonitrile, (Z)-I- bromobut-1 -ene (1.2 eq), Pd2(dba)3 (3 %) and tri(o-tolyl)phosphine (17 %) in DMF was degassed with N2 and heated at 80 0C over night. The mixture was diluted with sat. NH4Cl and EtOAc and the phases partitioned. The organic phase was washed with brine, dried over Na2SO4 and subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by 1H NMR.
Step (c): The title compound 2-((Z)-But-l-enyl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile was synthesized from the product of step (b) using a procedure anaologous to that described in Example 23. ES/MS m/z: 289.11 (M+H), 287.15 (M-H).
Example 39
2-(Isobut-l-enyl)-l-(4-hydroxyphenyl)-lH-indole-3-carbonitrile (E61)
Scheme 34
Figure imgf000052_0001
(a) Hexabutylditin, palladium di(triphenylphousphor)-dichloride, dioxane;
(b) 1-Bromo-methylprop-1-ene, Pd(PPh3)4, NMP/dioxane
Step (a): 1 eq of 2-bromo-l-(4-hydroxyphenyl)-lH-indole-3-carbonitrile (Example 1) was dissolved in 1,4-dioxan and stirred at room temperature. 1.9 eq of hexabutylditin and 0.04 eq of palladium di(triphenylphousphor)-dichloride were added, degassed with nitrogen, and the mixture was heated to 800C for 4 hours. The mixture was filtered to remove catalyst, evaporated to remove most of solvent, and the residue purified on a silica column using 75:25 «-heptane:ethyl acetate as mobile phase (yield 65%).
Step (b): 1 eq of the stannyl reagent as prepared in step (a) was dissolved in a mixed solvent (l-methyl-2- pyrrolodinone and 1 ,4-dioxan, 2:1), stirred at room temperature, 2 eq of 1 -bromo-methylprop-1 -ene and 0.2% (w/w) of tetrakis(triphenylphosphine) palladium were added, degassed with nitrogen, the mixture was heated to 8O0C overnight. The mixture was filtered to remove catalyst, evaporated to remove most of the solvent, and the residue purified with preparative HPLC. ES/MS m/z: 289.11 (M+H), 287.15 (M-H); 1H NMR (chloroform-d, 500MHz): 7.76 (m, IH), 7.28 (m, IH), 7.23 (m, IH), 7.17 (m, 2H), 7.13 (m, IH), 6.98 (m, 2H), 5.86 (m, IH), 1.98 (d, 3H, J=1.3Hz) and 1.89 (d, 3H, J=1.2Hz).
Example 40 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(2-methyl-allyl)-lH-indole-3-carbonitrile (E40)
Scheme 35
Figure imgf000052_0002
A mixture of 2-bromo-l-(2,3-difluoro-4-hydroxyphenyl)-lH-indole-3-carbonitrile (Example 103), methallyltri-n-butyltin (2 eq), Pd2(dba)3 (0.05 %) and tri(o-tolyl)phosphine (20 %) was dissolved in DMF, degassed with N2 and heated at 80 0C over night. The reaction mixture was quenched by addition of sat. NH4Cl, diluted with EtOAc, and washed with brine and then dried over Na2SO4. The residue was subjected to reversed phase preparative HPLC. Appropriate fractions were combined and evaporated, and identified by 1H NMR. ES/MS m/z: 289.11 (M+H), 287.15 (M-H); 1H NMR (chloroform-d, 500MHz): 7.76 (m, IH), 7.28 (m, IH), 7.23 (m, IH), 7.17 (m, 2H), 7.13 (m, IH), 6.98 (m, 2H), 5.86 (m, IH), 1.98 (d, 3H, J=I.3Hz) and 1.89 (d, 3H, J=I .2Hz).
Example 41
(Z)-2-(5-Ethyl-3-methylisoxazol-4-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3- carboximidamide (E41)
Scheme 36
Figure imgf000053_0001
(a) n-BuLi, MeI, THF; (b) BBr3, DCM; (c) NH2OH, Et3N, MeOH
Step (a): 38 μl of n-butyllithium (1.6M in hexanes) was added drop wise to 2-(3,5-dimethylisoxazol-4- yl)-l -(4-methoxyphenyl)-lH-indole-3-carbonitrile (synthesised by a coupling reaction of the intermediate product from step (b) of Example 1 with 3,5-dimethylisooxazole-4-boronic acid, using a process as described in Example 16) in 1 ml dry THF under argon at -78°C. The mixture was stirred at -78°C for 45 min. 34 μl of methyl iodide was added and the mixture was allowed to reach room temp. After 4 h the mixture was concentrated in vacuo and purified by silica gel (EtO Ac/heptane; 1 :1).
Step (b): The intermediate was prepared from 4-(l-(4-methoxyphenyl)-3-phenyl-lH-indol-2-yl)-3,5- dimethylisoxazole via a process anaologous to that described in step (c) of Example 1. Step (c): The title compound was prepared from 2-(5-ethyl-3-methylisoxazol-4-yl)-l-(4-hydroxyphenyl)- lH-indole-3-carbonitrile via a process anaologous to that described in Example 11. ES/MS m/z: 377.3 (M+H), 375.4 (M-H); 1H NMR (acetone-d6, 500MHz): 8.06 (m, IH), 7.22-7.10 (m, 5H), 6.94 (m, 2H), 2.56 (m, 2H), 2.02 (s, 3H) and 1.03 (t, 3H, J=7.3Hz).
Example 42 4-(2-(3,5-dimethylisoxazol-4-yl)-3-phenyl-lH-indol-l-yl)phenol (E42)
Scheme 37
Figure imgf000054_0001
(a) Phenylboronic acid, Pd(PPh3)4, K2CO3, NaI, DME/Dioxane (b) BBr3, DCM
Step (a): A mixture of DME/dioxane (1 :1) was degassed by freeze-pump-thaw three times and then put under an argon atmosphere. 10 mg of 4-(3-bromo-l-(4-methoxyphenyl)-lH-indol-2-yl)-3,5- dimethylisoxazole (the intermediate product of step (b) from the synthesis of Example 22), 6.1 mg phenyl boronic acid, 2.9 mg Pd(PPh3)4, 21 mg potassium carbonate and 7.6 mg NaI were put in a microwave vial that was sealed and flushed with argon. 0.7 ml of the DME/water solution was canulated to the microwave vial.
The mixture was degassed by freeze-pump-thaw twice and then flushed with argon. The mixture was heated to 150°C/15 min. in a Biotage Initiator micro wave oven. The crude product was filtered followed by evaporation and filtration through a short silica gel plug (EtOAc). Purification by flash chromatography (EtOAc/heptane; 1 :9 to 2:8) gave 22.2 mg as a mixture of debrominated starting material and product plus a small amount of unreacted starting material according to LCMS. The compounds separated well on HPLC but only one spot on TLC. The crude material was used directly in the following demethylation step.
Step (b): The title compound was prepared from 4-(l -(4-methoxyphenyl)-3 -phenyl- lH-indol-2-yl)-3, 5- dimethylisoxazole via a process anaologous to that described in step (c) of Example 1. ES/MS m/z: 381.2 (M+H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.43-7.37 (m, 4H), 7.30-7.19 (m, 4H), 7.17 (m, 2H), 6.96 (m, 2H), 1.99 (s, 3H) and 1.77 (s, 3H). Example 43 4-(3-chloro-2-(3,5-dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol (E43)
Scheme 38
Figure imgf000055_0001
(a) SO2CI2, DCM; (b) BF3Me2S, DCM
Step (a): To a solution of 4-(l-(4-methoxyphenyl)-lH-indol-2-yl)-3,5-dimethylisoxazole (the intermediate product of step (a) from the synthesis of Example 22, 7.5 mg, 0.024mmol) in DCM was added 1.5 equiv SO2CI2 and the mixture was stirred at rt for 1.5 h. The solution was concentrated to give a crude product, which was used directly in the next step.
Step (b): The title compound was prepared from 4-(l-(4-methoxyphenyl)-3-phenyl-lH-indol-2-yl)-3,5- dimethylisoxazole via a process anaologous to that described in Example 23. ES/MS m/z: 339.1 /341.1 (M+H), 337.2 /339.2 (M-H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.43-7.37 (m, 4H), 7.30-7.19 (m, 4H), 7.17 (m, 2H), 6.96 (m, 2H), 1.99 (s, 3H) and 1.77 (s, 3H).
Example 44 2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-sulfonamide (E 44)
Scheme 39
Figure imgf000055_0002
(a) HSO3CI, DCM;(b) 1. SOCI2 2. NH3, MeOH; (c) BF3MeS2, DCM Step (a): To a solution of 4-(l-(4-methoxyphenyl)-lH-indol-2-yl)-3,5-dimethylisoxazole (the intermediate product of step (a) from the synthesis of Example 22, 7.5 mg, 0.024mmol) in DCM was added 1.0 equiv HSO3Cl at 00C and the mixture was stirred at rt for 1 h. The solution was concentrated to give a raw product, which was used in the following step.
Step (b): To a solution of the raw product from step (a) in DCM was added 0.5 mL SOCl2 and the mixture was stirred at 700C for 15 min. The solution was concentrated and 2 mL saturated NH3 in MeOH was added and the mixture was stirred overnight. The solution was concentrated to give a raw product, which was used directly in the next step.
Step (c): The title compound was prepared from the raw product of step (b) using the process as described in Example 23. ES/MS m/z: 384.2 (M+H), 382.2 (M-H); 1H NMR (acetone-d6, 500MHz): 8.13 (m, IH), 7.32-7.28 (m, 2H), 7.19-7.15 (m, 3H), 6.93 (m, 2H), 2.24 (s, 3H) and 2.05 (s, 3H).
Example 45
2-(3,5-Dimethylisoxazol-4-yl)-l-(2-fluoro-4-hydroxyphenyl)-lH-indole-3-carboximidamide (E45)
Figure imgf000056_0001
0.5 mmol (Z)-2-(3,5-dimethylisoxazol-4-yl)-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-lH-indole-3- carboximidamide (Example 235) was dissolved in 5 ml acetic acid. Acetic acid anhydride (4 eq) was slowly added and the mixture was stirred over night. A Pd-C catalyst (10% on carbon) was added and the mixture was placed in a hydrogenation apparatus under 50 psi H2 over night. The mixture was filtered through celite and concentrated. The crude product was purified on HPLC: (Column: Sunfire, 20 min acidic gradient: 5-50% MeCN. ES/MS m/z: 365.15 (M+H), 363.18 (M-H); 1H NMR (MeOD, 500MHz): 7.86 (m, IH), 7.41-7.31 (m, 2.5H), 7.25-7.13 (m, 1.5H), 6.76 (m, IH), 6.67 (m, IH), 2.21, 2.15 (two s, 3H) and 2.08, 2.06 (two s, 3H). Examples 46-95
The following compounds were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method are described in Examples 1-5, 16 and 38-40 above.
Figure imgf000057_0001
E 46 1 -(4-Hydroxy-phenyl)-2 -phenyl- 1 H-indole-3 -carbonitrile
R1 = phenyl
ES/MS m/z: 311.1 (pos. M + H), 309.11 (neg. M - H); 1HNMR (CDCl3, 500MHz): 7.74 (m, IH), 7.31-7.15 (m, 8H), 7.00 (m, 2H) and 6.80 (m, 2H).
E 47 l-(4-Hydroxy-phenyl)-2-methyl-lH-indole-3-carbonitrile
R 1 = methyl
ES/MS m/z: 249.1 (pos. M + H), 247.3 (neg. M - H); 1H NMR (acetone-d6 , 500MHz): 7.63 (m, IH), 7.33 (m, 2H), 7.27 (m, IH), 7.23 (m, IH), 7.10 (m, 2H), 7.08 (m, IH) and 2.43 (s, 3H).
E 48 2-(3 -Cyano-thiophen-2-yl)- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile
R = 3 -cyano-thiophen-2-yl
ES/MS m/z: 342.1 (pos. M + H), 340.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.95 (d, IH, J=5.4Hz), 7.84 (m, IH), 7.52 (d, IH, J=5.4Hz), 7.46-7.42 (m, 2H), 7.32-7.28 (m, 3H) and 7.00 (m, 2H).
E 49 l-(4-Hydroxy-phenyl)-2-((E)-propenyl)-lH-indole-3-carbonitrile
R = (E)-propenyl
ES/MS m/z: 275.3 (pos. M + H), 273.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.65 (m, IH), 7.32-7.23 (m, 4H), 7.10 (m, 2H), 7.06 (m, IH), 6.85 (m, IH), 6.26 (m, IH) and 1.91 (dd, 3H, J=1.8, 6.9Hz).
E 50 l-(4-Hydroxy-phenyl)-2-thiophen-2-yl-lH-indole-3-carbonitrile R1 = thiophen-2-yl
ES/MS m/z: 317.0 (pos. M + H), 315.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.73 (m, IH), 7.65 (dd, IH, J=Ll, 5.0Hz), 7.54 (dd, IH, J=Ll, 3.8Hz), 7.37-7.29 (m, 4H), 7.15 (dd, IH, J=3.8, 5.0Hz), 7.11 (m, IH) and 7.06 (m, 2H).
E 51 2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 3,5-dimethyl-isoxazol-4-yl
ES/MS m/z: 330.2 (pos. M + H), 328.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.42-7.32 (m, 3H), 7.28 (m, 2H), 7.00 (m, 2H), 2.36 (s, 3H) and 2.02 (s, 3H).
E 52 l-(4-Hydroxy-phenyl)-2-pyridin-4-yl-lH-indole-3-carbonitrile
R = pyridine-4-yl
ES/MS m/z: 312.2 (pos. M + H), 310.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.35 (m, 2H), 7.38 (m, IH), 7.20 (m, 2H), 6.98 (m, 2H), 6.82 (m, IH), 6.79 (m, 2H) and 6.44 (m, 2H).
E 53 1 -(4-Hydroxy-phenyl)-2-( 1 -methyl-1 H-pyrrol-2-yl)- 1 H-indole-3 -carbonitrile
R . r1 _= 1 -methyl-1 H-pyrrol-2-yl
ES/MS m/z: 314.3 (pos. M + H), 312.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.75 (m, IH), 7.38-7.32 (m, 2H), 7.29 (m, IH), 7.20 (m, 2H), 6.95 (m, 2H), 6.83 (dd, IH, J=I .9, 2.5Hz), 6.24 (dd, IH, J=I .9, 3.8Hz), 6.09 (dd, IH, J=2.5, 3.8Hz) and 3.51 (s, 3H).
E 54 l-(4-Hydroxy-phenyl)-2-(3 -methyl -thiophen-2-yl)-l H-indole-3 -carbonitrile
R = 3-methyl-thiophen-2-yl
ES/MS m/z: 331.4 (pos. M + H), 329.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.77 (m, IH), 7.56 (d, IH, J=5.1Hz), 7.40-7.34 (m, 2H), 7.27 (m, IH), 7.21 (m, 2H), 6.97 (d, IH, J=5.1Hz), 6.95 (m, 2H) and 2.21 (s, 3H).
E 55 1 -(4-Hydroxy-phenyl)-2-isopropylamino- 1 H-indole-3 -carbonitrile
R = isopropylamino
ES/MS m/z: 292.1 (pos. M + H), 290.2 (neg. M - H); 'H NMR (acetone-d6, 500MHz): 7.34 (m, IH), 7.25 (m, 2H), 7.09 (m, IH), 7.06 (m, 2H), 6.95 (m, IH), 6.70 (m, IH), 4.33 (m, IH) and 1.28 (d, 6H, J=6.5Hz). E 56 2-Ethylamino- 1 -(4-hydroxy-phenyl)- 1 H-indole-3-carbonitrile
R = ethylamino
ES/MS m/z: 278.3 (pos. M + H), 276.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.33 (m, IH), 7.26 (m, 2H), 7.09 (m, IH), 7.06 (m, 2H), 6.95 (m, IH), 6.70 (m, IH), 3.65 (q, 2H, J=7.4Hz) and 1.27 (t, 3H, J=7.4Hz).
E 57 2-Butylamino- 1 -(4-hydroxy-phenyl)-l H-indole-3 -carbonitrile
R = butylamino
ES/MS m/z: 306.2 (pos. M + H), 304.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.33 (m, IH), 7.26 (m, 2H), 7.10-7.05 (m, 3H), 6.94 (m, IH), 6.69 (m, IH), 3.61 (m, 2H), 1.68 (m, 2H), 1.41 (m, 2H) and 0.92 (t, 3H, J=7.4Hz).
E 58 l-(4-Hydroxy-phenyl)-2-piperidin-l-yl-lH-indole-3-carbonitrile
R = piperidin-1-yl
ES/MS m/z: 317.9 (pos. M + H), 316.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.45 (m, IH), 7.36 (m, 2H), 7.18 (m, IH), 7.12-7.07 (m, 3H), 7.01 (m, IH), 3.27 (m, 4H), 1.54 (m, 2H) and 1.49 (m, 4H).
E 59 l-(4-Hydroxy-phenyl)-2-pyrrolidin-l-yl-lH-indole-3-carbonitrile
R = pyrrolidin-1-yl
ES/MS m/z: 304.4 (pos. M + H), 302.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.33-7.29 (m, 3H)1 7.11 (m, IH), 7.04 (m, 2H), 6.96 (m, IH), 6.73 (m, IH), 3.40 (m, 4H) and 1.87 (m, 4H).
E 60 l-(4-Hydroxy-phenyl)-2-moφholin-4-yl-lH-indole-3-carbonitrile
R = morpholin-4-yl
ES/MS m/z: 320.3 (pos. M + H), 317.8 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.49 (m, IH), 7.40 (m, 2H), 7.21 (m, IH), 7.14 (m, IH), 7.10 (m, 2H), 7.05 (m, IH), 3.59 (m, 4H) and 3.28 (m, 4H).
E 61 2-Diethylamino- 1 -(4-hydroxy-phenyl)- 1 H-indole-3-carbonitrile
R = diethylamino ES/MS m/z: 306.2 (pos. M + H), 304.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.47 (m, IH), 7.31 (m, 2H), 7.19 (m, IH), 7.10 (m, IH), 7.08 (m, 2H), 6.96 (m, IH), 3.28 (q, 4H, J=6.9Hz) and 1.05 (t, 6H, J=6.9Hz).
E 62 2-Ethynyl- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile
R1 = ethynyl
ES/MS m/z: 259.1 (pos. M + H), 257.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.74 (m, IH), 7.44-7.37 (m, 4H), 7.26 (m, IH), 7.10 (m, 2H) and 4.45 (s, IH).
E 63 1 -(4-Hydroxy-phenyl)-2 -vinyl- 1 H-indole-3 -carbonitrile
R1 = vinyl
ES/MS m/z: 261.1 (pos. M + H), 259.0 (neg. M - H); Η NMR (acetone-d6, 500MHz): 7.70 (m, IH), 7.34-7.27 (m, 4H), 7.13-7.09 (m, 3H), 6.56 (dd, IH, J=17.6, 11.6Hz), 6.24 (dd, IH, J=17.6, 0.6Hz) and 5.69 (dd, IH, J=I 1.6, 0.6Hz).
E 64 l-(4-Hydroxy-phenyl)-lH-indole-2,3-dicarbonitrile
R = cyano
ES/MS m/z: 258.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.8 8 (m, IH), 7.59 (m, IH), 7.56 (m, 2H) , 7.52 (m, IH), 7.42 (m, IH) and 7.15 (m, 2H).
Figure imgf000060_0001
E 66 1 -(4-Hydroxy-phenyl)-2-pyridin-2-yl- 1 H-indole-3 -carbonitrile
R = pyridine-2-yl
ES/MS m/z: 312.1 (pos. M + H), 310.2 (neg. M - H) T;T 1Hj NMR (acetone-d6, 500MHz): 8.63 (m, IH), 7.84-7.78 (m, 2H), 7.49 (m, IH), 7.41-7.36 (m, 3H), 7.26 (m, IH), 7.22 (m, 2H) and 6.96 (m, 2H).
E 67 l-(4-Hydroxy-phenyl)-2-(2-methyl-allyl)-l H-indole-3 -carbonitrile
R = 2-methyl-allyl ES/MS m/z: 289.1 (pos. M + H), 287.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.67 (m, IH), 7.31-7.24 (m, 4H), 7.10-7.05 (m, 3H), 4.76 (s, IH), 4.41 (s, IH), 3.55 (s, 2H) and 1.66 (s, 3H).
E 68 l-(4-Hydroxy-phenyl)-2-((Z)-propenyl)-lH-indole-3-carbonitrile
R 1 = (Z)-propenyl
ES/MS m/z: 275.1 (pos. M + H), 273.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz) : 7.71 (m, IH), 7.33-7.27 (m, 4H), 7.17 (m, IH), 7.06 (m, 2H), 6.20-6.12 (m, 2H) and 1.94 (d, 3H, J=5.3Hz)
E 69 2-(Butyl-methyl-amino)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R1 = Butyl-methyl-amino
ES/MS m/z: 320.3 (pos. M + H), 318.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.43 (m, IH), 7.31 (m, 2H), 7.17 (m, IH), 7.09-7.06 (m, 3H), 6.93 (m, IH), 3.15 (m, 2H), 3.00 (s, 3H), 1.45 (m, 2H), 1.20 (m, 2H) and 0.83 (t, 3H, J=7.4Hz).
E 70 l-(4-Hydroxy-phenyl)-2-((Z)-l-methyl-propenyl)-lH-indole-3-carbonitrile
R = (Z)-I -methyl -propenyl
ES/MS m/z: 289.2 (pos. M + H), 287.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.70 (m, IH), 7.33-7.27 (m, 4H), 7.22 (m, IH), 7.05 (m, 2H), 5.90 (m, IH), 1.78 (m, 3H) and 1.66 (m, 3H).
E 71 1 -(4-Hydroxy-phenyl)-2-imidazol-l -yl-lH-indole-3-carbonitrile
R = imidazol-1-yl
ES/MS m/z: 301.1 (pos. M + H), 299.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.85 (t, IH, J=0.9Hz), 7.79 (m, IH), 7.47-7.40 (m, 2H), 7.34-7.31 (m, 3H), 7.26 (m, IH), 7.06 (dd, IH, J=0.9, 1.6Hz) and 6.99 (m, 2H).
E 72 l-(4-Hydroxy-phenyl)-2-[l ,2,4]triazol-l-yl-lH-indole-3-carbonitrile
R = [l,2,4]triazol-l-yl
ES/MS m/z: 302.3 (pos. M + H), 300.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.73 (s, IH), 8.15 (s, IH), 7.84 (m, IH), 7.49-7.46 (m, 2H), 7.33-7.28 (m, 3H) and 6.99 (m, 2H).
E 73 2-(3,5-Dimethyl-pyrazol-l-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 3,5-dimethyl-pyrrazol-l-yl ES/MS m/z: 329.3(pos. M + H), 327.4 (neg. M - H); Η NMR (acetone-d6, 500MHz): 7.81 (m, IH), 7.46-7.42 (m, 2H), 7.35 (m, IH), 7.21 (m, 2H), 6.93 (m, 2H), 5.98 (s, IH), 2.15 (s, 3H) and 2.12 (s, 3H).
E 74 1 -(4-Hydroxy-phenyl)-2-pyrazol-l -yl- 1 H-indole-3 -carbonitrile
R = pyrrazol-1-yl
ES/MS m/z: 301.4 (pos. M + H), 299.5 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.89 (d, IH, J=2.6Hz), 7.79 (m, IH), 7.72 (d, IH, J=I .8Hz), 7.45-7.39 (m, 2H), 7.29-7.23 (m, 3H), 6.97 (m, 2H) and 6.47 (dd, IH, J=I.8, 2.6Hz).
E 75 l-(4-Hydroxy-phenyl)-2-(5-methyl-imidazol-l-yl)-lH-indole-3-carbonitrile
R = 5-methyl-imidazol-l-yl
ES/MS m/z: 315.2 (pos. M + H), 313.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.82 (m, IH), 7.76 (d, IH, J=0.9Hz), 7.47-7.43 (m, 2H), 7.33-7.27 (m, 3H), 6.97 (m, 2H), 6.76 (m, IH) and 2.13 (d, 3H, J=LlHz).
E 76 1 -(4-Hydroxy-phenyl)-2-(5 -methyl-pyrazol- 1 -yl)- 1 H-indole-3 -carbonitrile
R = 5 -methyl -pyrrazol-1-yl
ES/MS m/z: 315.2 (pos. M + H), 313.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.83 (m, IH), 7.55 (d, IH, J=I .3Hz), 7.47-7.44 (m, 2H), 7.37 (m, IH), 7.20 (m, 2H), 6.93 (m, 2H), 6.19 (m, IH) and 2.22 (s, 3H).
E 77 l-(4-Hydroxy-phenyl)-2-(3-methyl-pyrazol-l-yl)-lH-indole-3-carbonitrile
R = 3-methyl-pyrrazol-l-yl
ES/MS m/z: 315.2 (pos. M + H), 313.3 (pos. M + H); 1H NMR (acetone-d6, 500MHz): 7.76 (m, IH), 7.69 (d, IH, J=2.5Hz), 7.42-7.36 (m, 2H), 7.27-7.23 (m, 3H), 6.98 (m, 2H), 6.25 (d, IH, J=2.5Hz) and 2.21 (s, 3H).
E 78 l-(4-Hydroxy-phenyl)-2-thiazol-2-yl-lH-indole-3-carbonitrile
R = thiazol-2-yl
ES/MS m/z: 318.1 (pos. M + H), 316.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.99 (d, IH, J=3.2Hz), 7.81 (m, IH), 7.76 (d, IH, J=3.2Hz), 7.42-7.37 (m, 4H) and 7.15-7.11 (m, 3H). E 79 l-(4-Hydroxy-phenyl)-2-(2-methoxy-thiazol-4-yl)-lH-indole-3-carbonitrile
R1 = 2-methoxy-thiazol-4-yl
ES/MS m/z: 348.1 (pos. M + H), 346.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.75 (m, IH), 7.37-7.31 (m, 4H), 7.11 (m, IH), 7.07 (m, 2H), 6.68 (s, IH) and 4.04 (s, 3H).
E 80 l-(4-Hydroxy-phenyl)-2-thiazol-4-yl-lH-indole-3-carbonitrile
R = thiazol-4-yl
ES/MS m/z: 318.1 (pos. M + H), 316.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 9.08 (d, IH, J=I .9Hz), 7.78 (m, IH), 7.41 (d, IH, J=I.9Hz), 7.39-7.33 (m, 2H), 7.29 (m, 2H), 7.17 (m, IH) and 7.04 (m, 2H).
E 81 1 -(4-Hydroxy-phenyl)-2-(3 -methyl -but-2-enyl)- 1 H-indole-3 -carbonitrile
R' = 3-methyl-but-2-enyl
ES/MS m/z: 303.1 (pos. M + H), 301.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.64 (m, IH), 7.30 (m, 2H), 7.29-7.21 (m, 2H), 7.09 (m, 2H), 7.03 (m, IH), 5.11 (m, IH), 3.54 (m, 2H), 1.62 (m, 3H) and 1.48 (s, 3H).
Figure imgf000063_0001
E 83 l-(4-Hydroxy-phenyl)-2-(5-methyl-thiophen-2-yl)-lH-indole-3-carbonitrile
R = 5-methyl-thiophen-2-yl
ES/MS m/z: 331.1 (pos. M + H), 329.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.70 (m, IH), 7.35-7.28 (m, 5H), 7.09-7.05 (m, 3H), 6.82 (m, IH) and 2.45 (s, 3H).
E 84 2-(5-Acetyl-thiophen-2-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 5-acetyl-thiophen-2-yl
ES/MS nVz: 359.3 (pos. M + H), 357.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.85 (d, IH, J=4.1Hz), 7.77 (m, IH), 7.57 (d, IH, J=4.1Hz), 7.41-7.34 (m, 4H), 7.15 (m, IH), 7.08 (m, 2H) and 2.54
Figure imgf000064_0001
E 85 1 -(4-Hydroxy-phenyl)-2-( 1 -methyl- 1 H-pyrrazol-4-yl)- 1 H-indole-3 -carbonitrile
R = I -methyl- 1 H-pyrrazol-4-yl
ES/MS m/z: 315.2 (pos. M + H), 313.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.73 (s, IH), 7.67 (m, IH), 7.35 (s, IH), 7.32-7.28 (m, 3H), 7.26 (m, IH), 7.09 (m, 2H), 7.07 (m, IH) and 3.90 (s, 3H).
E 86 2-(5-Chloro-thiophen-2-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 5-chloro-thiophen-2-yl
ES/MS m/z: 351.2 (pos. M + H), 349.3 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.74 (m, IH), 7.45 (d, IH, J=4.1Hz), 7.39-7.32 (m, 4H) and 7.13-7.08 (m, 4H).
E 87 l-(4-Hydroxy-phenyl)-2-(4-methyl-thiophen-3-yl)-lH-indole-3-carbonitrile
R1 = 4-methyl-thiophen-3 -yl
ES/MS m/z: 331.4 (pos. M + H), 329.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.76 (m, IH), 7.59 (d, IH, J=3.2Hz), 7.39-7.33 (m, 2H), 7.29 (m, IH), 7.21 (m, 2H), 7.18 (m, IH), 6.93 (m, 2H) and 2.08 (d, 3H, J=0.9Hz).
E 88 l-(4-Hydroxy-phenyl)-2-(4-methyl-thiophen-2-yl)-l H-indole-3 -carbonitrile
R = 4-methyl-thiophen-2-yl
ES/MS m/z: 331.4 (pos. M + H), 329.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.71 (m, IH), 7.39 (d, IH, J=I .3Hz), 7.36-7.29 (m, 2H), 7.27 (m, 2H), 7.22 (m, IH), 7.08 (m, IH), 7.04 (m, 2H) and 2.23 (d, 3H, J=0.9Hz).
E 89 2-(4-Cyano-thiophen-3 -yl)- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile
R = 4-cyano-thiophen-3-yl
ES/MS m/z: 342.2 (pos. M + H), 340.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.49 (d, IH, J=3.1Hz), 7.98 (d, IH, J=3.1Hz), 7.81 (m, IH), 7.44-7.39 (m, 2H), 7.31 (m, IH), 7.26 (m, 2H) and 6.97 (m, 2H).
E 90 l-(4-Hydroxy-phenyl)-2-(2-methyl-2H-pyrazol-3-yl)-lH-indole-3-carbonitrile R = 2-methyl-2H-pyrrazol-3-yl
ES/MS m/z: 315.2 (pos. M + H), 313.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.80 (m, IH), 7.45 (d, IH, J=2.0Hz), 7.43-7.39 (m, 2H), 7.32 (m, IH), 7.27 (m, 2H), 6.97 (m, 2H), 6.42 (d, IH, J=2.0Hz) and 3.77 (s, 3H).
E 91 l-(4-Hydroxy-phenyl)-2-(l,3,5-trimethyl-lH-pyrrazol-4-yl)-lH-indole-3-carbonitrile
R1 = l,3,5-trimethyl-lH-pyrrazol-4-yl
ES/MS m/z: 343.1 (pos. M + H), 341.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.73 (m, IH), 7.36-7.30 (m, 3H), 7.17 (m, 2H), 6.94 (m, 2H), 3.69 (s, 3H), 2.11 (s, 3H) and 1.96 (s, 3H).
E 92 2-(2-Acetyl-pyrrol- 1 -yl)-l -(4-hydroxy -phenyl)- 1 H-indole-3 -carbonitrile
R = 2-acetyl-pyrrol-l -yl
ES/MS m/z: 342.2 (pos. M + H), 340.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.76 (m, IH), 7.44 (dd, IH, J=1.6, 2.8Hz), 7.42-7.37 (m, 2H), 7.23 (m, IH), 7.13 (dd, IH, J=I .6, 3.9Hz), 7.09 (m, 2H), 6.88 (m, 2H), 6.41 (dd, IH, J=2.8, 3.9Hz) and 2.23 (s, 3H).
E 93 2-(2 -Ethyl-pyrrol- 1 -yl)- 1 -(4-hydroxy-phenyl)-l H-indole-3 -carbonitrile
R = 2-ethyl-pyrrol-l-yl
ES/MS m/z: 328.4 (pos. M + H), 326.2 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.78 (m, IH), 7.44-7.39 (m> 2H), 7.30 (m, IH), 7.22 (m, 2H), 6.93 (m, 2H), 6.88 (dd, IH, J=2.9, 1.6Hz), 6.1 1 (t, IH, J=3.2Hz), 5.95 (m, IH), 2.49 (m, IH), 2.37 (m, IH) and 1.09 (t, 3H, J=7.5Hz).
E 94 2-(2-Cyano-pyrrol-l-yl)-l-(4-hydroxy-phenyl)-lH-mdole-3-carbonitrile
R = 2-cyano-pyrrol-l-yl
ES/MS m/z: 325.4 (pos. M + H), 323.5 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.85 (m, IH), 7.49-7.46 (m, 3H), 7.32 (m, IH), 7.28 (m, 2H), 7.11 (dd, IH, J=4.1, 1.6Hz), 6.99 (m, 2H) and 6.45 (dd, IH, J=4.1, 2.9Hz).
E 95 l-(4-Hydroxy-phenyl)-2-(2-methyl-pyrrol-l-yl)-lH-indole-3-carbonitrile
R = 2 -methyl -pyrrol- 1 -yl
ES/MS m/z: 314.3 (pos. M + H), 312.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.44.7.39 (m, 2H), 7.31 (m, IH), 7.22 (m, 2H), 6.94 (m, 2H), 6.85 (dd, IH, J=3.0, 1.6Hz), 6.07 (t, IH, J=3.3Hz), 5.92 (m, IH) and 2.12 (d, 3H, J=0.7Hz).
Examples 96-159
The following compounds were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method are described in Examples 1-5, 16 and 38-40 above.
Figure imgf000066_0001
E 96 1 -(3 -Chloro-5 -fluoro-4-hydroxy-phenyl)-2-phenyl-l H-indole-3 -carbonitrile
R' = phenyl R = 3-chloro-5-fiuoro-4-hydroxy-phenyl
ES/MS m/z: 363.1 (pos. M + H), 361.1 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.77 (m, IH), 7.56-7.53 (m, 2H), 7.50-7.45 (m, 3H), 7.41-7.34 (m, 4H) and 7.32 (dd, IH, J=10.8, 2.5Hz).
E 97 l-(3-Chloro-5-fluoro-4-hydroxy-ρhenyl)-2-(3-cyano-thiophen-2-yl)-lH-indole-3-carbonitrile
R = 3-cyano-thiophen-2-yl R = 3-chloro-5-fluoro-4-hydroxy-phenyl
ES/MS m/z: 392.0, 394.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.00 (d, IH, J=5.4Hz), 7.84 (m, IH), 7.56 (d, IH, J=5.4Hz) and 7.49-7.41 (m, 5H).
E 98 1 -(3 -Chloro-5 -fluoro-4-hydroxy-phenyl)-2-(3 -cyano-furan-2-yl)- 1 H-indole-3 -carbonitrile
R = 3-cyano-furan-2-yl R = 3 -chloro-5 -fluoro-4-hydroxy-phenyl
ES/MS m/z: 376.0, 378.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.97 (d, IH, J=2.0Hz), 7.86 (m, IH), 7.52-7.44 (m, 5H) and 7.07 (d, IH, J=2.0Hz).
E 99 2-Bromo-l-(3-chloro-5-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = bromo R = 3-chloro-5-fluoro-4-hydroxy-phenyl
ES/MS m/z: 362.0, 364.0, 366.0 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.70 (m, IH), 7.54 (t, IH, J=2.5Hz), 7.50 (dd, IH, J=2.5, 10.7Hz), 7.39-7.34 (m, 2H) and 7.27 (m, IH). E lOO 2-Bromo-l-(2-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
= bromo R = 2-fluoro-4-hydroxy-phenyl
ES/MS m/z: 331.1, 333.2 (pos. M + H), 328.9, 331.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.71 (m, IH), 7.48 (m, IH), 7.38-7.33 (m, 2H), 7.14 (m, IH) and 6.98-6.93 (m, 2H).
E lOl l-(2-Fluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile
R1 = phenyl R = 2-fluoro-4-hydroxy-phenyl
ES/MS m/z: 329.3 (pos. M + H), 327.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.52-7.49 (m, 2H), 7.47-7.44 (m, 3H), 7.42-7.36 (m, 3H), 7.19 (m, IH), 6.84 (m, IH) and 6.77 (dd, IH, J=2.7, 11.7Hz).
E 102 2-Bromo-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = bromo R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 331.1, 333.2 (pos. M + H), 329.2, 331.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.70 (m, IH), 7.46 (dd, IH, J=2.5, 11.4Hz), 7.38-7.32 (m, 2H), 7.31-7.25 (m, 2H) and 7.21 (m, IH).
E 103 2-Bromo- 1 -(2,3 -difluoro-4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile
R = bromo R = 2,3 -difluoro-4-hydroxy-phenyl
ES/MS m/z: 349.1, 351.2 (pos. M + H), 346.9, 349.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.72 (m, IH), 7.40-7.34 (m, 3H), 7.22 (m, IH) and 7.14 (m, IH).
E 104 2-Bromo-l-(2,5-difluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = bromo R = 2,5-difluoro-4-hydroxy-phenyl
ES/MS m/z: 349.1, 351.2 (pos. M + H), 346.9, 349.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.73 (m, IH), 7.61 (dd, IH, J=7.2, 10.6Hz), 7.41-7.35 (m, 2H), 7.23 (m, IH) and 7.16 (dd, IH, J=7.6, 10.7Hz).
E 105 2-Bromo-l-(3-chloro-4-hydroxy-ρhenyl)-lH-indole-3-carbonitrile
R = bromo R = 3-chloro-4-hydroxy-phenyl
ES/MS m/z: 347.0, 349.1, (pos. M + H), 344.8, 347.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.70 (m, IH), 7.65 (d, IH, J=2.5Hz), 7.39 (dd, IH, J=2.5, 8.5Hz), 7.38-7.32 (m, 2H), 7.30 (d, IH, J=8.5Hz) and 7.20 (m, IH). E 106 2-Bromo- 1 -(3 ,5 -difluoro-4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile
R = bromo R = 3,5-difluoro-4-hydroxy-phenyl
ES/MS m/z: 349.1, 351.2 (pos. M + H), 347.2, 349.3 (neg. M - H)J 1H NMR (acetone-d6, 500MHz): 7.70 (m, IH), 7.40-7.34 (m, 4H) and 7.27 (m, IH).
E 107 l-(3-Fluoro-4-hydroxy-phenyl)-2-ρhenyl-lH-indole-3-carbonitrile
R = phenyl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 329.3 (pos. M + H), 327.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.77 (m, IH), 7.51 (m, 2H), 7.46-7.43 (m 3H), 7.40-7.35 (m, 2H), 7.31-7.26 (m, 2H) and 7.14-7.07 (m, 2H).
E 108 1 -(3 ,5 -Difluoro-4-hydroxy-phenyl)-2 -phenyl- 1 H-indole-3 -carbonitrile
R = phenyl R = 3,5-difluoro-4-hydroxy-phenyl
ES/MS m/z: 347.2 (pos. M + H), 344.8 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.77 (m, IH), 7.53 (m, 2H), 7.49-7.46 (m, 3H), 7.42-7.35 (m, 3H) and 7.19 (m, 2H).
E 109 1 -(3 -Chloro-4-hydroxy-phenyl)-2 -phenyl- 1 H-indole-3 -carbonitrile
R1 = phenyl R = 3-chloro-4-hydroxy-phenyl
ES/MS m/z: 344.9, 347.3 (pos. M + H), 343.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.77 (m, IH), 7.52 (m, 2H), 7.48 (d, IH, J=2.5Hz), 7.47-7.44 (m, 3H), 7.41-7.35 (m, 2H), 7.29 (m, IH), 7.22 (dd, IH, J=2.5, 8.8Hz) and 7.13 (d, IH, J=8.8Hz).
E IlO l-(2,3-Difluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile
R = phenyl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 347.2 (pos. M + H), 344.8 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.80 (m, IH), 7.52 (m, 2H), 7.49-7.46 (m, 3H), 7.43-7.38 (m, 2H), 7.29-7.25 (m, 2H) and 7.00 (m, IH).
E lIl l-(2,5-Difluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile
R = phenyl R = 2,5-difluoro-4-hydroxy-phenyl
ES/MS m/z: 347.3 (pos. M + H), 344.8 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.79 (m, IH), 7.54-7.51 (m, 3H), 7.50-7.46 (m, 3H), 7.43-7.37 (m, 2H), 7.27 (m, IH) and 6.96 (dd, IH, J=I.6, 10.7Hz). E 112 1 -(3 ,5 -Difluoro-4-hydroxy-phenyl)-2-thiophen-3 -yl- 1 H-indole-3 -carbonitrile
RJ = thiophen-3-yl R = 3,5-difluoro-4-hydroxy-phenyl
ES/MS m/z: 353.0 (pos. M + H), 351.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.74 (m, IH), 7.71 (dd, IH, J=I .3, 2.9Hz), 7.59 (dd, IH, J=2.9, 5.1Hz), 7.40-7.34 (m, 2H), 7.30 (m, IH) and 7.24-7.20 (m, 3H).
E 113 1 -(3,5 -Difluoro-4-hydroxy-phenyl)-2-thiophen-2~yl- 1 H-indole-3 -carbonitrile
R1 = thiophen-2-yl R = 3,5-difluoro-4-hydroxy-phenyl
ES/MS m/z: 353.0 (pos. M + H), 351.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.74 (m, IH), 7.71 (dd, IH, J=I.2, 5.0Hz), 7.54 (dd, IH, J=1.2, 3.8Hz), 7.40-7.34 (m, 2H), 7.30 (m, 2H), 7.25 (m, IH) and 7.19 (dd, IH, J=3.8, 5.0Hz).
E 114 l-(3,5-Difluoro-4-hydroxy-phenyl)-2-(3,5-dimethyl-isoxazol-4-yl)-lH-indole-3-carbonitrile
R = 3,5-dimethyl-isoxazol-4-yl R = 3,5-difluoro-4-hydroxy-phenyl
ES/MS m/z: 366.2 (pos. M + H), 364.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.45 (m, IH), 7.44-7.40 (m, 2H), 7.22 (m, 2H), 2.41 (s, 3H) and 2.09 (s, 3H).
E 115 1 -(3 ,5 -Difluoro-4-hydroxy-phenyl)-2-( 1 -methyl- 1 H-pyrrol-2-yl)- 1 H-indole-3 -carbonitrile
R = 1 -methyl- 1 H-pyrrol-2-yl R = 3, 5 -difluoro-4 -hydroxy-phenyl
ES/MS m/z: 350.3 (pos. M + H), 348.1 (neg. M - H); 1H NMR (acetone-dβ, 500MHz): 7.76 (m, IH), 7.41-7.36 (m, 3H), 7.15 (m, 2H), 6.90 (dd, IH, J=I .8, 2.6Hz), 6.27 (dd, IH, J=1.8, 3.8Hz), 6.12 (dd, IH, J=2.6, 3.8Hz) and 3.61 (s, 3H).
E 116 l-(3,5-Difluoro-4-hydroxy-phenyl)-2-(3-methyl-thiophen-2-yl)-lH-indole-3-carbonitrile
R = 3-methyl-thiophen-2-yl R = 3,5-difluoro-4-hydroxy-phenyl
ES/MS m/z: 367.1 (pos. M + H), 365.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.77 (m, IH), 7.61 (d, IH, J=5.1Hz), 7.42-7.36 (m, 3H), 7.16 (m, 2H), 7.02 (d, IH, J=5.1Hz) and 2.26 (s, 3H).
E 117 l-(3,5-Difluoro-4-hydroxy-phenyl)-2-(l-methyl-lH-pyrazol-4-yl)-lH-indole-3-carbonitrile
R = 1 -methyl- lH-pyrrazol-4-yl R = 3,5-difluoro-4-hydroxy-phenyl ES/MS m/z: 351.5 (pos. M + H), 349.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.79 (s, IH), 7.68 (m, IH), 7.50 (s, IH), 7.35-7.28 (m, 2H), 7.25 (m, 2H), 7.18 (m, IH) and 3.91 (s, 3H).
E 118 l-(3,5-Difluoro-4-hydroxy-phenyl)-2-pyridm-4-yl-lH-indole-3-carbonitrile
R = pyridine-4-yl R = 3,5-difluoro4-hydroxy-phenyl
ES/MS m/z: 348.2 (pos. M + H); 1H NMR (acetone-d6, 500MHz): 8.70 (m, 2H), 7.81 (m, IH), 7.49 (m, 2H), 7.45-7.39 (m, 3H) and 7.24 (m, 2H).
E 119 1 -(3 -Chloro-4-hydroxy-phenyl)-2-thiophen-3 -yl-1 H-indole-3 -carbonitrile
R = thiophen-3-yl R = 3-chloro-4-hydroxy-phenyl
ES/MS m/z: 351.2 (pos. M + H), 349.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.74 (m, IH), 7.67 (dd, IH, J=I.3, 2.8Hz), 7.57 (dd, IH, J=2.8, 5.1Hz), 7.52 (d, IH, J=2.5Hz), 7.39-7.32 (m, 2H), 7.27 (dd, IH, J=2.5, 8.6Hz), 7.23 (m, IH), 7.20 (d, IH, J=8.6Hz) and 7.18 (dd, IH, J=1.3, 5.1Hz).
E 120 l-(3-Chloro-4-hydroxy-phenyl)-2-thiophen-2-yl-lH-indole-3-carbonitrile
R = thiophen-2-yl R = 3-chloro-4-hydroxy-phenyl
ES/MS m/z: 351.2 (pos. M + H), 349.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.74 (m, IH), 7.68 (dd, IH, J=1.3, 5.0Hz), 7.58 (d, IH, J=2.5Hz), 7.56 (dd, IH, J=1.3, 3.8Hz), 7.39-7.31 (m, 3H), 7.24 (d, IH, J=8.5Hz) and 7.18-7.15 (m, 2H).
E 121 l-(3-Chloro-4-hydroxy-phenyl)-2-(3,5-dimethyl-isoxazol-4-yl)-lH-indole-3-carbonitrile
R = 3,5-dimethyl-isoxazol-4-yl R = 3-chloro-4-hydroxy-phenyl
ES/MS m/z: 364.4 (pos. M + H), 362.5 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.53 (br s, IH), 7.43-7.36 (m, 3H), 7.27 (br d, IH, J=7.7Hz), 7.19 (d, IH, J=8.9Hz), 2.39 (s, 3H) and 2.06 (s, 3H).
E 122 1 -(3 -Chloro-4-hydroxy-phenyl)-2-( 1 -methyl- 1 H-pyrrol-2-yl)- 1 H-indole-3 -carbonitrile
R = 1 -methyl- 1 H-pyrrol-2-yl R = 3-chloro-4-hydroxy-phenyl
ES/MS m/z: 348.2 (pos. M + H), 346.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.75 (m, IH), 7.43 (d, IH, J=2.5Hz), 7.40-7.35 (m, 2H), 7.32 (m, IH), 7.22 (dd, IH, J=2.5, 8.7Hz), 7.14 (d, IH, J=8.7Hz), 6.87 (dd, IH, J=I .9, 2.8Hz), 6.25 (dd, IH, J=1.9, 3.8Hz), 6.10 (dd, IH, J=2.8, 3.8Hz) and 3.58 (s, 3H). E 123 1 -(3 -Chloro-4-hydroxy-phenyl)-2-(3 -methyl -thiophen-2-yl)- 1 H-indole-3 -carbonitrile
R1 = 3-methyl-thiophen-2-yl R = 3-chloro-4-hydroxy-phenyl
ES/MS m/z: 365.0 (pos. M + H), 363.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.77 (m, IH), 7.59 (d, IH, J=5.1Hz), 7.46 (d, IH, J=2.6Hz), 7.41-7.36 (m, 2H), 7.30 (m, IH), 7.22 (dd, IH, J=2.6, 8.5Hz), 7.14 (d, IH, J=8.5Hz), 7.00 (d, IH, J=5.1Hz) and 2.24 (s, 3H).
E 124 l-(3-Chloro-4-hydroxy-phenyl)-2-pyridin-4-yl-lH-indole-3-carbonitrile
R = pyridine-4-yl R = 3-chloro-4-hydroxy-phenyl
ES/MS m/z: 346.1 (pos. M + H), 343.9 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.68 (m, 2H), 7.81 (m, IH), 7.55 (d, IH, J=2.5Hz), 7.47 (m, 2H), 7.44-7.41 (m, 2H), 7.34 (m, IH), 7.27 (dd, IH, J=2.5, 8.6Hz) and 7.16 (d, IH, J=8.6Hz).
E 125 l-(3-Fluoro-4-hydroxy-phenyl)-2-thiophen-3-yl-lH-indole-3-carbonitrile
R = thiophen-3-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 335.6 (pos. M + H), 333.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.74 (m, IH), 7.67 (dd, IH, J=I .3, 2.8Hz), 7.56 (dd, IH, J=2.8, 5.0Hz), 7.38-7.30 (m, 3H), 7.24 (m, IH), 7.21-7.17 (m, 2H) and 7.13 (m, IH).
E 126 l-(3-Fluoro-4-hydroxy-phenyl)-2-thiophen-2-yl-lH-indole-3-carbonitrile
R = thiophen-2-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 335.6 (pos. M + H), 333.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.73 (m, IH), 7.68 (dd, IH, J=1.3, 5.0Hz), 7.54 (dd, IH, J=1.3, 3.8Hz), 7.39-7.32 (m, 3H) and 7.25-7.16 (m, 4H).
E 127 2-(3,5-Dimethyl-isoxazol-4-yl)-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 3,5-Dimethyl-isoxazol-4-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 348.2 (pos. M + H), 346.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.79 (m, IH), 7.43-7.39 (m, 3H), 7.32 (m, IH), 7.20-7.13 (m, 2H), 2.39 (s, 3H) and 2.05 (s, 3H).
E 128 1 -(3 -Fluoro-4-hydroxy-phenyl)-2-( 1 -methyl- 1 H-pyrrol-2-yl)- 1 H-indole-3 -carbonitrile
R = l-methyl-lH-pyrrol-2-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 332.3 (pos. M + H), 330.1 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.75 (m, IH), 7.40-7.32 (m, 3H), 7.21 (dd, IH, J=2.1, 11.3Hz), 7.15-7.08 (m, 2H), 6.87 (dd, IH, J=1.8, 2.5Hz), 6.25
Figure imgf000072_0001
E 129 1 -(3 -Fluoro-4-hydroxy-phenyl)-2-(3 -methyl -thiophen-2-yl)- 1 H-indole-3 -carbonitrile
R1 = 3-methyl-thiophen-2-yl R = 3-fluoro-4-hydroxy-plienyl
ES/MS m/z: 349.4 (pos. M + H), 347.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.77 (m, IH), 7.59 (d, IH, J=5.1Hz), 7.41-7.36 (m, 2H), 7.32 (m, IH), 7.25 (dd, IH, J=2.4, 11.4Hz), 7.15-7.08 (m, 2H), 7.00 (d, IH, J=5.1Hz) and 2.24 (s, 3H).
E 130 l-(3-Fluoro-4-hydroxy-phenyl)-2-(l-methyl-lH-pyrrazol-4-yl)-lH-indole-3-carbonitrile
R = 1 -methyl- lH-pyrrazol-4-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 333.2 (pos. M + H), 331.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.76 (br s, IH), 7.68 (m, IH), 7.41 (d, IH, J=0.6Hz), 7.36-7.23 (m, 4H), 7.16 (m, IH), 7.12 (m, IH) and 3.91 (s, 3H).
E 131 l-(3-Fluoro-4-hydroxy-phenyl)-2-pyridin-4-yl-lH-indole-3-carbonitrile
R = pyridine-4-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 330.2 (pos. M + H), 328.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.68 (m, 2H), 7.81 (m, IH), 7.47 (m, 2H), 7.44-7.40 (m, 2H), 7.37-7.33 (m, 2H) and 7.17-7.12 (m, 2H).
E 132 2-Dimethylamino-l-(2-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = dimethylamino R = 2-fluoro-4-hydroxy-phenyl
ES/MS m/z: 296.3 (pos. M + H), 294.1 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.45-7.41 (m, 2H), 7.19 (m, IH), 7.10 (m, IH), 6.69-6.85 (m, 3H) and 2.96 (s, 6H).
E 133 2-(3,5-Dimethyl-isoxazol-4-yl)-l -(2-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 3,5-dimethyl-isoxazol-4-yl R = 2-fTuoro-4-hydroxy-phenyl
ES/MS m/z: 348.2 (pos. M + H), 346.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.80 (m, IH), 7.47 (m, IH), 7.42 (m, IH), 7.27 (m, IH), 6.88 (m, IH), 6.83 (m, IH), 2.34, 2.33 (2 s, 3H) and 2.10, 2.09 (2 s, 3H).
E 134 l-(3-Fluoro-4-hydroxy-phenyl)-2-((E)-propenyl)-lH-indole-3-carbonitrile
R = (E)-proρenyl R = 3-fluoro-4-hydroxy-phenyl ES/MS m/z: 293.1(pos. M + H), 291.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.66 (m, IH), 7.35-7.25 (m, 4H), 7.16 (m, IH), 7.11 (m, IH), 6.86 (m, IH), 6.29 (m, IH) and 1.92 (dd, 3H, J=I.9, 6.9 Hz).
E 135 l-(3-Fluoro-4-hydroxy-phenyl)-2-((Z)-propenyl)-lH-indole-3-carbonitrile
R = (Z)-propenyl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 293.1(pos. M + H), 291.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.71 (m, IH), 7.35-7.29 (m, 3H), 7.25-7.21 (m, 2H), 7.16 (m, IH), 6.23-6.16 (m, 2H) and 1.94 (m, 3H).
E 136 1 -(2,3 -Difluoro-4-hydroxy-phenyl)-2-((Z)-propenyl)- 1 H-indole-3 -carbonitrile
R = (Z)-propenyl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 311.0 (pos. M + H), 309.1 (neg. M - H); 1H NMR (CDCl3, 500MHz): 7.79 (m, IH), 7.35- 7.28 (m, 2H), 7.08 (m, IH), 7.05-6.96 (m, 2H), 6.17 (m, IH), 6.10 (m, IH) and 1.98 (dd, 3H, J=1.5, 6.9Hz).
E 137 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-vinyl-lH-indole-3-carbonitrile
R = vinyl R = 2,3-difhioro-4-hydroxy-phenyl
ES/MS m/z: 297.0 (pos. M + H), 295.1 (neg. M - H); 1H NMR (CDCl3, 500MHz): 7.78 (m, IH), 7.34- 7.27 (m, 2H), 7.08-6.99 (m, 3H), 6.46 (dd, IH, J=I 1.7, 17.8Hz), 6.31 (d, IH, J=17.8Hz) and 5.67 (d, IH, J=I 1.7Hz).
E 138 1 -(2,3 -Difluoro-4-hydroxy-phenyl)-2-thiophen-3 -yl- 1 H-indole-3 -carbonitrile
R = thiophen-3-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 353.3 (pos. M + H), 351.4 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.77 (m, IH), 7.70 (dd, IH, J=I .3, 2.8Hz), 7.61 (dd, IH, J=2.8, 5.0Hz), 7.41-7.35 (m, 2H), 7.31 (m, IH), 7.25 (dd, IH, J=I.3, 5.0Hz), 7.23 (m, IH) and 7.06 (m, IH).
E 139 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-thiophen-2-yl-lH-indole-3-carbonitrile
R = thiophen-2-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 353.3 (pos. M + H), 351.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.76 (m, IH), 7.71 (m, IH), 7.60 (m IH), 7.42-7.32 (m, 3H), 7.21-7.19 (m, 2H) and 7.10 (m, IH). E 140 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(3-methyl-thiophen-2-yl)-lH-indole-3-carbonitrile
R1 = 3-methyl-thiophen-2-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 367.1 (pos. M + H), 365.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.80 (m, IH), 7.60 (d, IH, J=5.1Hz), 7.44-7.39 (m, 2H9, 7.27 (m, IH), 7.22 (m, IH), 7.02 (d, IH, J=5.1Hz), 6.99 (m, IH) and 2.28 (s, 3H).
E 141 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(l-methyl-lH-pyrrol-2-yl)-lH-indole-3-carbonitrile
R1 = l-methyl-lH-pyrrol-2-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 350.3 (pos. M + H), 348.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.78 (m, IH), 7.42-7.37 (m, 2H), 7.26-7.22 (m, 2H), 6.99 (m, IH), 6.93 (dd, IH, J=I.5, 2.9Hz)5 6.14 (dd, IH, J=I .5, 3.8Hz), 6.08 (dd, IH, J=2.9, 3.8Hz) and 3.70 (s, 3H).
E 142 2-(2-Acetyl-pyrrol-l-yl)-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R' = 2-acetyl-ρyrrol-l-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 360.2 (pos. M + H), 358.2 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 8.31 (m, IH), 7.41 (d, IH, J=5.2Hz), 7.32-7.26 (m, 2H), 7.18 (m, IH), 7.16-6.96 (m, 3H), 6.85 (d, IH, J=5.2Hz) and 2.09 (s, 3H).
E 143 1 -(3 -Fluoro-4-hydroxy-phenyl)-2-pyrτol- 1 -yl- 1 H-indole-3 -carbonitrile
R = pyrrol- 1-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 317.9 (pos. M + H), 316.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.74 (m, IH), 7.43-7.37 (m, 2H), 7.31-7.29 (m, 2H), 7.16-7.14 (m, 2H), 6.97 (t, 2H, J=2.2Hz) and 6.26 (t, 2H, J=2.2Hz).
E 144 1 -(2,3 -Difluoro-4-hydroxy-phenyl)-2 -pyrrol- 1 -yl- 1 H-indole-3 -carbonitrile
R = pyrrol- 1-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 336.5 (pos. M + H), 334.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.77 (m, IH), 7.46-7.40 (m, 2H), 7.31 (m, IH), 7.27 (m, IH), 7.02 (m, IH), 6.99 (t, 2H, J=2.2Hz) and 6.28 (t, 2H, J=2.2Hz).
E 145 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-prop-l-ynyl-lH-indole-3-carbonitrile
R = prop-1 -ynyl R = 2,3-difluoro-4-hydroxy-phenyl ES/MS m/z: 309.1 (pos. M + H), 307.2 (neg. M - H); 1H NMR (CDCl3, 500MHz): 7.74 (m, IH), 7.34- 7.30 (m, 2H), 7.12 (m, IH), 7.05 (m, IH), 6.98 (m, IH) and 2.08 (s, 3H).
E 146 l-(3-Fluoro-4-hydroxy-phenyl)-2-(2-methyl-prop-l-enyl)-lH-indole-3-carbonitrile
R = 2-methyl-prop-l-enyl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 307.1 (pos. M + H),305.2 (neg. M - H); 1H NMR (CDCl3, 500MHz): 7.76 (m, IH), 7.40- 7.36 (m, 2H), 7.18-7.13 (m, 2H), 7.07 (m, IH), 7.02 (m, IH), 5.86 (m, IH), 1.98 (d, 3H, J=I .3Hz) and 1.91 (d, 3H, J=I .4Hz).
E 147 1 -(2,3 -Difluoro-4-hydroxy-phenyl)-2-(2 -methyl-prop- 1 -enyl)-l H-indole-3 -carbonitrile
R = 2-methyl-prop-l-enyl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 325.1 (pos. M + H), 323.2 (neg. M - H); 1H NMR (CDCl3, 500MHz): 7.77 (m, IH), 7.33- 7.25 (m, 2H), 7.05-7.01 (m, 2H), 6.96 (m, IH), 5.85 (m, IH), 1.96 (d, 3H, J=I .2Hz) and 1.91 (d, 3H, J=I .3Hz).
E 148 2-(2-Acetyl-pyrrol-l-yl)-l-(2,3-difluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 2-acetyl-pyrrol-l-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 378.2 (pos. M + H), 376.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): two conformations in ratio 2:1, 7.82-7.77 (m, 3H), 7.52 (dd, IH, J=2.9, 1.6Hz), 7.47-7.42 (m, 6H), 7.35-7.34 (m, 2H), 7.31 (m, IH), 7.27-7.25 (m, 2H), 7.20 (dd, 2H, J=4.1, 1.6Hz), 7.16 (dd, IH, J=4.0, 1.6Hz), 7.03-6.99 (m, 2H), 6.96-6.88 (m, 3H), 6.81 (m, IH), 6.47 (dd, IH, J=4.0, 2.8Hz), 6.44 (dd, 2H, J=3.8, 2.8Hz), 2.29 (s, 6H) and 2.21 (s, 3H).
E 149 l-(3-Fluoro-4-hydroxy-phenyl)-2-pyrazol-l-yl-lH-indole-3-carbonitrile
R = pyrrazol-1-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 319.1 (pos. M + H), 317.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.98 (dd, IH, 3=2.6, 0.6Hz), 7.79 (m, IH), 7.73 (d, IH, J=I .8Hz), 7.46-7.41 (m, 2H), 7.33 (m, IH), 7.28 (m, IH), 7.15-7.09 (m, 2H) and 6.51 (dd, IH, J=2.5, 1.7Hz).
E 150 1 -(2,3 -Difluoro-4-hydroxy-phenyl)-2-pyrazol- 1 -yl- 1 H-indole-3 -carbonitrile
R = pyrrazol-1-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 337.4 (pos. M + H), 335.5 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.14 (d, IH, J=2.6Hz), 7.82 (m, IH), 7.72 (d, IH, J=I .6Hz), 7.48-7.43 (m, 2H), 7.31 (m, IH), 7.20 (m, IH), 6.96 (m, IH) and 6.56 (dd, IH, J=2.6, 1.6Hz).
E 151 2-(2,5-Dimethyl-pyrrol- 1 -yl)-l -(3 -fluoro-4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile
R = 2,5-dimethyl-pyrrol-l-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 346.1 (pos. M + H), 344.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.82 (m, IH), 7.46-7.43 (m, 3H), 7.15-7.10 (m, 2H), 7.04 (m, IH), 5.83 (s, 2H) and 2.06 (s, 6H).
E 152 2-(2-Ethyl-pyrrol-l -yl)-l -(3 -fluoro-4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile
R = 2-ethyl-pyrrol-l-yl R = 3 -fluoro-4-hydroxy-phenyl
ES/MS m/z: 346.1 (pos. M + H), 344.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.79 (m, IH), 7.45-7.41 (m, 2H), 7.37 (m, IH), 7.22 (m, IH), 7.13-7.09 (m, 2H), 6.92 (dd, IH, J=2.9, 1.6Hz), 6.14 (t, IH, J=3.3Hz), 5.98 (m, IH), 2.50 (m, IH), 2.40 (m, IH) and 1.10 (t, 3H, J=7.6Hz).
E 153 2-(2-Cyano-pyrrol-l-yl)-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 2-cyano-pyrrol-l-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 343.1 (pos. M + H), 341.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.86 (m, IH), 7.52-7.48 (m, 3H), 7.39 (m, IH), 7.32 (m, IH), 7.19-7.12 (m, 3H) and 6.48 (dd, IH, J=3.9, 2.8Hz).
E 154 l-(3-Fluoro-4-hydroxy-phenyl)-2-(2-methyl-pyrrol-l-yl)-lH-mdole-3-carbonitrile
R = 2-methyl-pyrrol-l-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 332.3 (pos. M + H), 330.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.79 (m, IH), 7.45-7.41 (m, 2H), 7.37 (m, IH), 7.23 (m, IH), 7.14-7.09 (m, 2H), 6.88 (dd, IH, J=3.1, 1.7Hz), 6.09 (t, IH, J=3.1Hz), 5.95 (m, IH) and 2.14 (d, IH, J=0.9Hz).
E 155 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(2-ethyl-pyrrol-l-yl)-lH-indole-3-carbonitrile
R = 2-ethyl-pyrrol-l-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 364.4 (pos. M + H), 362.5 (neg. M - H); 1H NMR (acetone-d6, 500MHz): two confoπnations in ratio 1.5:1, 7.83-7.80 (m, 2.5H), 7.48-7.43 (m, 5H), 7.33-7.28 (m, 4H), 7.10 (m, IH), 6.99-6.90 (m, 4H), 6.73 (m, IH), 6.14-6.11 (m, 2.5H), 6.00-5.99 (m, 2.5H), 2.59-2.46 (m, 3.5H), 2.36 (m, 1.5H) and 1.17-1.13 (m, 7.5H). E 156 2-(2-Cyano-pyrrol-l-yl)-l-(2,3-difluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile
R = 2-cyano-pyrrol-l-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 361.4 (pos. M + H), 359.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.88 (m, IH), 7.55-7.50 (m, 2H), 7.46-7.33(m, 3H), 7.17 (dd, IH, J=4.0, 1.5Hz), 7.01 (m, IH), and 6.49 (dd, IH, J=3.8, 2.9Hz).
E 157 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(2-methyl-pyrrol-l-yl)-lH-mdole-3-carbonitrile
R = 2-methyl-pyrrol-l-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 350.3 (pos. M + H), 348.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.82 (m, IH), 7.48-7.44 (m, 2H), 7.37 (m, IH), 7.30 (m, IH), 7.09-6.85 (m, 2H), 6.08 (m, IH), 5.97 (m, IH) and 1.20 (s, 3H).
E 158 l-(2-Fluoro-4-hydroxy-phenyl)-2-pyrrol-l-yl-lH-indole-3-carbonitrile
R = pyrrol- 1-yl R = 2-fluoro-4-hydroxy-phenyl
ES/MS m/z: 317.9 (pos. M + H), 316.3 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.76 (m, IH), 7.47 (t, IH, J=8.8Hz), 7.44-7.38 (m, 2H), 7.19 (m, IH), 6.96 (m, 2H), 6.86 (m, IH), 6.81 (dd, IH, J=I 1.5, 2.5Hz) and 6.27 (m, 2H).
E 159 l-(2,3-difluoro-4-hydroxyphenyl)-2-(3-methylbut-2-enyl)-lH-indole-3-carbonitrile
R = 3-methylbut-2-enyl R = 2,3-difluoro-4-hydroxyphenyl
ES/MS m/z: 317.9 (pos. M + H), 316.3 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 7.76 (m, IH), 7.47 (t, IH, J=8.8Hz), 7.44-7.38 (m, 2H), 7.19 (m, IH), 6.96 (m, 2H), 6.86 (m, IH), 6.81 (dd, IH, J=I 1.5, 2.5Hz) and 6.27 (m, 2H).
Examples 160-195
Examples 160-162 below were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 160-162 are described in Examples 1-4, 8, 16 and 38-40 above.
Examples 163-168 and 170-172 below were prepared according to General Method 4 above. Full experimental details of the individual steps of that general method are described in Examples 2-4, 9, and 20-21 above. Examples 173-195 below were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 173-195 are described in Examples 1-4, 10-11, 14-16, 38-40 and 45 above.
Figure imgf000078_0001
E 160 [l-(4-Hydroxy-phenyl)-2-phenyl-lH-indol-3-yl]-acetonitrile
R1 = phenyl R = cyanomethyl
ES/MS m/z: 325.1 (pos. M + H), 323.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.81 (m, IH), 7.41-7.32 (m, 5H), 7.25-7.22 (m, 2H), 7.19 (m, IH), 7.11 (m, 2H), 6.88 (m, 2H) and 3.97 (s, 2H).
E 161 [ 1 -(4-Hydroxy-phenyl)-2 -phenyl- 1 H-indol-3-yl] -acetic acid
R = phenyl R2 = carboxymethyl
ES/MS m/z: 344.1 (pos. M + H), 342.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.71 (m, IH), 7.39-7.27 (m, 5H), 7.17.7.12 (m, 3H), 7.07 (m, 2H), 6.86 (m, 2H) and 3.74 (s, 2H).
E 162 2-[l-(4-Hydroxy-phenyl)-2-phenyl-lH-indol-3-yl]-acetamide
R1 = phenyl R = carbamoyl
ES/MS m/z: 313.1 (pos. M + H), 311.2 (neg. M - H); 1H NMR (CDCl3, 500MHz): 7.68 (m, IH), 7.32- 7-23 (m, 6H), 7.20-7.18 (m, 2H), 7.06 (m, 2H), 6.81 (m, 2H) and 3.77 (s, 2H).
E 163 4-(3-Isopropenyl-2-phenyl-indol-l-yl)-phenol
R' = phenyl R = isopropenyl
ES/MS m/z: 326.2 (pos. M + H), 324.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.72 (m, IH), 7.31-7.25 (m, 5H), 7.16-7.11 (m, 3H), 7.07 (m, 2H), 6.86 (m, 2H), 5.27 (m, IH), 5.15 (m, IH) and 1.83 (m, 3H).
E 164 4-[3-(2-Methyl-2H-pyrazol-3-yl)-2-phenyl-indol-l-yl]-phenol
R = phenyl R2 = 2-Methyl-2H-pyrrazol-3-yl ES/MS m/z: 366.2 (pos. M + H), 364.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.47-7.45 (m, 2H), 7.24-7.13 (m, 10H), 6.92 (m, 2H), 6.32 (d, IH, J= 1.9Hz) and 3.33 (s, 3H).
E 165 4-(2-Phenyl-3-thiazol-4-yl-indol-l-yl)-phenol
R1 = phenyl R2 = thiazol-4-yl
ES/MS m/z: 369.1 (pos. M + H), 367.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 9.06 (d, IH, J=2.0Hz), 8.30 (m, IH), 7.35-7.31 (m, 5H), 7.22-7.18 (m, 2H), 7.16-7.12 (m, 3H), 6.87 (m, 2H) and 6.81 (d, IH, J=2.2Hz).
E 166 4-(2-Phenyl-3-prop-l -ynyl-indol-1 -yl)-phenol
R1 = phenyl R = prop-l-ynyl
ES/MS m/z: 324.2 (pos. M + H), 322.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.70 (m, IH), 7.48-7.46 (m, 2H), 7.34-7.26 (m, 3H), 7.21-7.18 (m, 2H), 7.16 (m, IH), 7.11 (m, 2H), 6.91 (m, 2H) and 2.07 (s, 3H).
E 167 1 -(4-Hydroxy-phenyl)-2-((E)-propenyl)-lH-indole-3-carboxylic acid amide
R1 = (E)-propenyl R = carbamoyl
ES/MS m/z: 293.1 (pos. M + H), 291.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.99 (m, IH), 7.19 (m, 2H), 7.16-7.10 (m, 2H), 7.05 (m, 2H), 6.94 (m, IH), 6.73 (m, IH), 5.91 (m, IH) and 1.73 (dd, 3H, J=I.3, 6.6Hz).
E 168 1 -(4-Hydroxy-phenyl)-2-(2-methyl-prop-l -enyl)-lH-indole-3-carboxylic acid amide
R = 2-methyl-prop-l-enyl R = carbamoyl
ES/MS m/z: 307.2 (pos. M + H), 305.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.36 (m, IH), 7.20 (m, 2H), 7.17-7.12 (m, 2H), 7.05 (m, IH), 7.01 (m, 2H), 6.17 (m, IH), 1.83 (d, 3H, J=I .4Hz) and 1.65 (d, 3H, J=I .2Hz).
E 169 1 -(4-Hydroxy-phenyl)-2-((Z)-l -methyl-propenyl)-lH-indole-3-carboxylicacid amide
R = (Z)-I -methyl-propenyl R = carbamoyl
ES/MS m/z: 307.1 (pos. M + H), 305.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.41 (m, IH), 7.23 (br s, 2H), 7.19-7.13 (m, 2H), 7.03-7.00 (m, 3H), 5.91 (m, IH), 1.89 (m, 3H) and 1.59 (m, 3H). E 170 4-(2-Phenyl-3-pyrrazol-l -yl-indol-1 -yl)-phenol
R = phenyl R2 = pyrrazol-1-yl
ES/MS m/z: 352.1 (pos. M + H), 350.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.68 (d, IH, J=I .3Hz), 7.57 (m, IH), 7.51 (dd, IH, J=0.6, 2.5Hz), 7.27-7.17 (m, 10H), 6.92 (m, 2H) and 6.34 (t, IH, J=2.1Hz).
E 171 4-(3-Imidazol-l -yl-2-phenyl-indol-l -yl)-phenol
R1 = phenyl R2 = imidazol-1-yl
ES/MS m/z: 352.2 (pos. M + H), 350.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.75 (br s , 1H), 7.53 (m, IH), 7.38-7.29 (m, 7H), 7.25-7.19 (m, 5H) and 6.91 (m, 2H).
E 172 4-[3-(5-Methyl-pyrazol-l-yl)-2-phenyl-indol-l-yl]-phenol
R = phenyl R = 5-Methyl-pyrrazol-l-yl
ES/MS m/z: 366.1 (pos. M + H), 364.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.61 (m, IH), 7.34 (d, IH, J=2.1Hz), 7.25-7.15 (m, 10H), 6.91 (m, 2H), 6.11 (d, IH, J=2.1Hz) and 2.29 (s, 3H).
E 173 2-Bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid amide
R = bromo R2 = carbamoyl
ES/MS m/z: 331.0, 333.0 (pos. M + H), 329.0, 331.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.22 (m, IH), 7.28 (m, 2H), 7.22-7.17 (m, 2H), 7.08 (m, 2H) and 7.00 (m, IH).
E 174 l-(4-Hydroxy-phenyl)-2-((Z)-3,3,3-trifluoro-propenyl)-lH-indole-3-carbonitrile
R = (Z)-3,3,3-trifluoro-propenyl R2 = CN
ES/MS m/z: 329.1 (pos. M + H), 327.13 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.75 (m, IH), 7.39-7.34 (m, 2H), 7.31 (m, 2H), 7.23 (m, IH), 7.08 (m, 2H), 7.03 (d, IH, J=12.2Hz) and 6.43 (m, IH).
E 175 (Z)-2-bromo-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide
R = bromo R = N-Hydroxycarbamimidoyl
ES/MS m/z: 346.1, 347.9 (pos. M + H), 343.9, 346.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.91-8.84 (s, OH), 7.98-7.95 (m, IH), 7.27-7.23 (m, 2H), 7.17-7.10 (m, 2H), 7.09-7.05 (m, 2H), 7.00- 6.97 (m, IH), 5.49-5.38 (m, 2H). E 176 (Z)-N'-hydroxy- 1 -(4-hydroxyphenyl)-2-(l H-pyrrol- 1 -yl)-l H-indole-3 -carboximidamide
R = pyrrol- 1-yl R = N-Hydroxycarbamimidoyl
ES/MS m/z: 333.2 (pos. M + H), 331.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.25-8.22 (m, IH), 8.14-8.12 (s, OH), 7.25-7.16 (m, 4H), 7.10-7.06 (m, IH), 6.92-6.90 (t, 2H), 6.90-6.86 (m, 2H), 6.16-6.14 (t, 2H), 4.71-4.53 (m, 2H).
E 177 2-(3,5-Dimethyl-isoxazol-4-yl)-l -(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid amide
R = 3,5-Dimethyl-isoxazol-4-yl R = carbamoyl
ES/MS m/z: 348.2 (pos. M + H); 1H NMR (acetone-d6, 500MHz): 8.26 (m, IH), 7.27-7.23 (m, 2H), 7.21-7.08 (m, 3H), 6.95 (m, 2H), 2.20 (s, 3H) and 2.06 (s, 3H).
E 178 (Z)-N'-hydroxy- 1 -(4-hydroxyphenyl)-2-(2-methylprop- 1 -enyl)- 1 H-indole-3 -carboximidamide
R1 = 2-methyl-prop-l-enyl R = N-Hydroxycarbamimidoyl
ES/MS m/z: 322.4 (pos. M + H), 320.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.14 (s, IH), 7.99 (m, IH), 7.29 (m, 2H), 7.13-7.05 (m, 5H) and 1.35 (s, 6H).
E 179 1 -(4-Hydroxy-phenyl)-2 -phenyl- 1 H-indole-3 -carboxylic acid hydroxyamide
R1 = phenyl R = hydroxycarbamoyl
ES/MS m/z: 345.13 (pos. M + H), 343.13 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.04 (m, IH), 7.38 (m, 2H), 7.34-7.31 (m, 3H), 7.23-7.20 (m, 2H), 7.13-7.08 (m, 3H) and 6.87 (m, 2H).
E 180 (Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-phenyl-lH-indole-3-carboximidamide
R = phenyl R = N-Hydroxycarbamimidoyl
ES/MS m/z: 344 (pos. M + H), 342.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.03 (m, IH), 7.39 (m, 2H), 7.31-7.28 (m, 3H), 7.18-7.13 (m, 2H), 7.09-7.06 (m, 3H) and 6.85 (m, 2H).
E 181 1 -(4-Hydroxy-phenyl)-2-pyrrol-l -yl-1 H-indole-3 -carboxylic acid amide
R = pyrrol- 1-yl R = carbamoyl
ES/MS m/z: 318.15 (pos. M + H), 316.19 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.44 (m, IH), 7.29-7.23 (m, 4H), 7.10 (m, IH), 7.00 (t, 2H, J=2.1Hz), 6.90 (m, 2H) and 6.24 (t, 2H, J=2.1Hz). E 182 [l-(4-Hydroxy-phenyl)-2-pyrrol-l-yl-lH-indol-3-yl]-carbamic acid tert-butyl ester
R = pyrrol- 1-yl R = tør£-butoxycarbonylamino
ES/MS m/z: 390.16 (pos. M + H), 388.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 7.59 (m, IH), 7.22-7.15 (m, 3H), 7.11 (m, 2H), 6.89 (m, 2H), 6.77 (m, 2H), 6.09 (m, 2H) and 1.45 (s, 9H).
E 183 2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-N-methyl-lH-indole-3-carboxamidine
R = 3,5-Dimethyl-isoxazol-4-yl R = N-methylcarbamimidoyl
ES/MS m/z: 361.2 (pos. M + H), 359.2 (neg. M - H); 1H NMR (methanol-d4, 500MHz): 7.81 (m, IH), 7.38-7.34 (m, 3H), 7.07 (br s, 2H), 6.91 (m, 2H), 3.11 (s, 3H), 2.14 (s, 3H) and 1.96 (s, 3H).
E 184 methyl 2-(3 ,5 -dimethylisoxazol-4-yl)- 1 -(4-hydroxyphenyl)- lH-indole-3 -carbimidate
R = 3,5-Dimethyl-isoxazol-4-yl R2 = imino(methoxy)methyl
ES/MS m/z: 362.3 (pos. M + H), 360.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz):8.26 (m, IH), 7.27-7.04 (m, 5H), 6.96 (m, 2H), 3.04 (s, 3H), 2.21 (s, 3H) and 2.07 (s, 3H).
E 185 N-((2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3- yl)(imino)methyl)acetamide
R1 = 3,5-Dimethyl-isoxazol-4-yl R2 = (imino)methylacetamide
ES/MS m/z: 1H NMR (acetone-d6, 500MHz): 8.38 (m, IH), 7.34-7.29 (m, 2H), 7.25-7.20 (m, 3H), 6.97 (m, 2H), 2.59 (s, 3H), 2.17 (s, 3H) and 1.97 (s, 3H).
E 186 2-(5-ethyl-3-methylisoxazol-4-yl)-l -(4-hydroxyρhenyl)-lH-indole-3-carboxamide
R = 5-ethyl-3-methylisoxazol-4-yl R2 = carbamoyl
ES/MS m/z: 362.3 (pos. M + H), 360.4 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.27 (m, IH), 7.27-7.07 (m, 5H), 6.96 (m, 2H), 2.58 (m, 2H), 2.09 (s, 3H) and 1.04 (t, 3H, J=7.6Hz).
E 187 (Z)-2-(2-ethyl-lH-pyrrol-l-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3- carboximidamide
R1 = 2-ethyl-lH-pyrrol-l-yl R = N-Hydroxycarbamimidoyl
ES/MS m/z: 361.23 (pos. M + H), 359.35 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.34 (d, IH, J=7.9Hz), 7.24 (m, IH), 7.20-7.17 (m, 3H), 7.11 (m, IH), 6.91-6.87 (m, 3H), 6.13 (t, IH, J=3.2Hz), 5.91 (m, IH), 2.35 (m, IH), 2.25 (m, IH) and 1.06 (t, 3H, J=7.6Hz). E 188 (Z)-Nl-hydroxy-l-(4-hydroxyphenyl)-2-(2-methyl-lH-pyrrol-l-yl)-lH-indole-3- carboximidamide
R = 2-methyl-lH-pyrrol-l-yl R = N-Hydroxycarbamimidoyl
ES/MS m/z: 347.18 (pos. M + H), 345.25 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.33 (d, IH, J=7.8Hz), 7.24 (m, IH), 7.20-7.17 (m, 3H), 7.12 (m, IH), 6.91-6.87 (m, 3H), 6.08 (t, IH, J=3.0Hz), 5.88 (m, IH) and 1.99 (s, 3H).
E 189 l-(4-hydroxyphenyl)-2-(2-metliyl-lH-pyrrol-l-yl)-lH-indole-3-carboxamide
R = 2-methyl-lH-pyrrol-l-yl R = carbamoyl
ES/MS m/z: 332.16 (pos. M + H), 330.21 (neg. M - H); 1H NMR (acetone-dβ, 500MHz): 8.50 (m, IH), 7.31-7.27 (m, 2H), 7.25 (m, 2H), 7.14 (m, IH), 6.98 (m, IH), 6.93 (m, 2H), 6.16 (t, IH, J=3.4Hz), 5.96 (m, IH) and 2.00 (s, 3H).
E 190 4-(3-chloro-2-(3,5-dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol
R = 3,5-dimethylisoxazol-4-yl R' = C1
ES/MS m/z: 339.1 /341.1 (pos. M + H), 337.2 /339.2 (neg. M - H); 1H NMR (CDCl3, 500MHz): 7.72 (m, IH), 7.32-7.27 (m, 3H), 7.02 (m, 2H9, 6.86 (m, 2H), 2.24 (s, 3H) and 1.99 (s, 3H).
E 191 (Z)-2-((Z)-but-2-en-2-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide
R = (Z)-but-2-en-2-yl R2 = N-Hydroxycarbamimidoyl
ES/MS m/z: 322.19 (pos. M + H), 320.27 (neg. M - H); 1H NMR (methanol-d3, 500MHz): 7.69 (m, IH), 7.30-7.24 (m, 2H), 7.19-7.10 (m, 3H), 6.94 (m, 2H), 5.85 (m, IH), 1.79 (t, 3H, J=1.3Hz) and 1.57 (m, 3H).
E 192 (Z)-N'-hydroxy- 1 -(4-hydroxyphenyl)-2-(5 -methyl- 1 H-pyrazol- 1 -yl)-l H-indole-3 - carboximidamide
R = 5-methyl-lH-pyrazol-l-yl R2 = N-Hydroxycarbamimidoyl
ES/MS m/z: 348.22 (pos. M + H), 346.23 (neg. M - H); 1H NMR (methanol-d3, 500MHz): 8.10 (d, IH, J=8.1Hz), 7.59 (d, IH, J=I .9Hz), 7.32-7.21 (m, 3H), 7.08 (m, 2H), 6.78 (m, 2H), 6.14 (m, IH) and 2.02 (s, 3H).
E 193 (Z)-N'-hydroxy-l -(4-hydroxyphenyl)-2-(4-methyltliioplien-3-yl)-lH-indole-3- carboximidamide R = 4-methylthiophen-3-yl R = N-Hydroxycarbamimidoyl
ES/MS m/z: 364.17 (pos. M + H), 362.2 (neg. M - H); 1H NMR (methanol-d3, 500MHz): 7.88 (m, IH), 7.33 (d, IH, J=3.2Hz), 7.23-7.20 (m, 2H), 7.17 (m, IH), 7.02-6.99 (m, 3H), 6.78 (m, 2H) and 1.99 (s, 3H).
E 194 (Z)-2-(2,5-dimethyl-lH-pyrrol-l-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3- carboximidamide
R = 2,5-dimethyl-lH-pyrrol-l-yl R = N-Hydroxycarbamimidoyl
ES/MS m/z: 361.4 (pos. M + H), 359.5 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.38 (m, IH), 7.25 (m, IH), 7.21-7.18 (m, 2H), 7.12 (m, 2H), 6.91 (m, 2H), 5.80 (s, 2H) and 2.00 (s, 6H).
E 195 (Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-phenoxy-lH-indole-3-carboximidamide
R = phenoxy R = N-Hydroxycarbamimidoyl
ES/MS m/z: 360.18 (pos. M + H), 358.22 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.24 (m, IH), 7.25-7.14 (m, 6H), 7.09 (m, IH), 7.00 (m, IH) and 6.90-6.85 (m, 4H).
Examples 196-210
Examples 196-210 below were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 196-210 are described in Examples 1-4, 8, 16 and 38-40 above.
Figure imgf000084_0001
E 196 1 -(4-Hydroxy-phenyl)-2-phenyl-lH-indole-3-carboxylic acid
R = phenyl R = carboxy
ES/MS m/z: 330.1 (pos. M + H), 328.1 (neg. M - H); 'H NMR (acetone-d6, 500MHz): 8.31 (m, IH), 7.38-7.36 (m, 2H), 7.29-7.24 (m, 4H), 7.22 (m, IH), 7.11-7.08 (m, 3H) and 6.88 (m, 2H).
E 197 2-(3,5-Dimethyl-isoxazol-4-yl)-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carboxylic acid R m = 3,5-Dimethyl-isoxazol-4-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 367.1 (pos. M + H), 365.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.31 (m, IH), 7.33-7.28 (m, 2H), 7.23 (m, IH), 7.21-6.95 (m, 3H), 2.19 (s, 3H) and 2.07 (s, 3H).
E 198 2-(3,5-Dimethyl-isoxazol-4-yl)-l-(2-fluoro-4-hydroxy-phenyl)-lH-indole-3-carboxylic acid
R = 3,5-Dimethyl-isoxazol-4-yl R = 2-fluoro-4-hydroxy-phenyl
ES/MS m/z: 367.1 (pos. M + H), 365.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.32 (d, IH, J=7.3Hz), 7.46 (t, IH, J=8.8Hz), 7.34-7.25 (m, 2H), 7.13 (m, IH), 6.89-6.77 (m, 2H), 2.16, 2.15 (2s, 3H) and 2.07, 2.06 (2s, 3H).
E 199 l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(3,5-dimethyl-isoxazol-4-yl)-lH-indole-3-carboxylic acid
R = 3,5-Dimethyl-isoxazol-4-yl R = 2,3-difluoro-4-hydroxy-phenyl
ES/MS m/z: 385.1 (pos. M + H), 383.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.32 (d, IH, J=7.6Hz), 7.36-7.28 (m, 3H), 7.17 (m, IH), 7.03 (m, IH), 2.20, 2.17 (2s, 3H) and 2.08, 2.05 (2s, 3H).
E 200 l-(4-Hydroxy-phenyl)-2-((Z)-propenyl)-lH-indole-3-carboxylic acid
R = (Z)-propenyl R= 4-hydroxy-phenyl
ES/MS m/z: 294.1 (pos. M + H), 292.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.24 (m, IH), 7.25 (m, 2H), 7.23 (m, IH), 7.19 (m, IH), 7.14 (m, IH), 7.03 (m, 2H), 6.42 (m, IH), 5.86 (m, IH) and 1.46 (dd, 3H, J=1.9, 7.1Hz).
E 201 1 -(4-Hydroxy-phenyl)-2-((E)-propenyl)- 1 H-indole-3 -carboxylic acid
R = (E)-propenyl R= 4-hydroxy-phenyl
ES/MS m/z: 294.1 (pos. M + H), 292.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.20 (m, IH), 7.24 (m, 2H), 7.22-7.18 (m, 2H), 7.15 (m, IH), 7.08 (m, 2H), 6.94 (m, IH), 5.72 (m, IH) and 1.72 (dd, 3H, J=I.8, 6.7Hz).
E 202 l-(4-Hydroxy-phenyl)-2-(2 -methyl-prop- l-enyl)-l H-indole-3 -carboxylic acid
R = 2-methyl-prop-l -enyl R = 4-hydroxy-phenyl
ES/MS m/z: 308.1 (pos. M + H), 306.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.23 (m, IH), 7.24-7.20 (m, 3H), 7.17 (m, IH), 7.12 (m, IH), 7.02 (m, 2H), 6.17 (m, IH), 1.75 (d, 3H, J=1.3Hz) and
Figure imgf000086_0001
E 203 l-(4-Hydroxy-phenyl)-2-(2-methyl-allyl)4H-indole-3-carboxylic acid
R1 = 2-methyl-allyl R = 4-hydroxy-phenyl
ES/MS m/z: 308.1 (pos. M + H), 306.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.21 (m, IH), 7.24 (m, 2H), 7.21 (m, IH), 7.15 (m, IH), 7.05 (m, 2H), 6.97 (m, IH), 4.65 (m, IH), 4.24 (m, IH), 3.83 (s, 2H) and 1.62 (s, 3H).
E 204 1 -(4-Hydroxy-phenyl)-2-((Z)-l -methyl-propenyl)-lH-indole-3-carboxylic acid
R = (Z)-I -methyl -propenyl R = 4-hydroxy-phenyl
ES/MS m/z: 308.1 (pos. M + H), 306.2 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.24 (m, IH), 7.26-7.21 (m, 3H), 7.17 (m, IH), 7.05 (m, IH), 7.02 (m, 2H), 5.59 (m, IH), 1.92 (m, 3H) and 1.39 (m, 3H).
E 205 1 -(3 -Fluoro-4-hydroxy-phenyl)-2-thiophen-3 -yl-1 H-indole-3 -carboxylic acid
R = thiophen-3-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 354.2 (pos. M + H), 352.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.29 (m, IH), 7.48 (dd, IH, J=1.3, 2.9Hz), 7.36 (dd, IH, J=3.2, 5.1Hz), 7.28 (m, IH), 7.24 (m, IH), 7.17-7.12 (m, 3H), 7.08 (dd, IH, J=8.4, 9.3Hz) and 7.00 (m, IH).
E 206 1 -(3 -Fluoro-4-hydroxy-phenyl)-2-thiophen-2-yl- 1 H-indole-3 -carboxylic acid
R = thiophen-2-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 354.2 (pos. M + H), 352.3 (neg. M - H); 1H NMR (acetone-dό, 500MHz): 8.29 (m, IH), 7.54 (dd, IH, J=I .3, 5.0Hz), 7.31-7.25 (m, 2H), 7.22-7.19 (m, 2H), 7.13 (m, IH), 7.09 (m, IH), 7.04 (m, IH) and 7.00 (dd, IH, J=3.5, 5.0Hz).
E 207 1 -(3-Fluoro-4-hydroxy-phenyl)-2-(l -methyl-lH-pyrrol-2-yl)-lH-indole-3-carboxylic acid
R1 = l-methyl-lH-pyrrol-2-yl R = 3 -fluoro-4-hydroxy -phenyl
ES/MS m/z: 351.5 (pos. M + H), 349.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.30 (m, IH), 7.31-7.25 (m, 2H), 7.17 (m, IH), 7.16-6.93 (m, 3H), 6.75 (m, IH) and 5.96-5.93 (m, 2H).
E 208 l-(3-Fluoro-4-hydroxy-phenyl)-2-(3-methyl-thiophen-2-yl)-lH-indole-3-carboxylic acid R = 3-methyl-thiophen-2-yl R = 3-fluoro-4-hydroxy-phenyl
ES/MS m/z: 368.0 (pos. M + H), 366.1 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.31 (m, IH), 7.41 (d, IH, J=5.1Hz), 7.32-7.26 (m, 2H), 7.18 (m, IH), 7.16-6.97 (m, 3H), 6.85 (d, IH, J=5.1Hz) and 2.09 (s, 3H).
E 209 2-Bromo-l -(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid
R = bromo R = 4-hydroxy-phenyl
ES/MS m/z: 332.0, 334.0 (pos. M + H), 330.0, 332.0 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.22 (m, IH), 7.32-7.20 (m, 4H) and 7.12-7.01 (m, 3H).
E 210 1 -(4-Hydroxy-phenyl)-2-pyrrol-l -yl-lH-indole-3-carboxylic acid
R = pyrrol- 1-yl R = carboxy
ES/MS m/z: 319.15 (pos. M + H), 317.16 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 8.03 (m, IH), 7.39 (m, 2H), 7.31-7.28 (m, 3H), 7.18-7.13 (m, 2H), 7.09-7.06 (m, 3H) and 6.85 (m, 2H).
Example 211
Example 211 below was prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Example 211 are described in Examples 1-5, 16 and 38-40 above.
Figure imgf000087_0001
Figure imgf000087_0002
Examples 212-228
Examples 212-228 below were prepared according to General Method 2 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 212-228 are described in Examples 2-4, 8, 16, 24 and 38-40 above.
Figure imgf000088_0001
Figure imgf000088_0002
Figure imgf000088_0003
Figure imgf000088_0004
Figure imgf000088_0005
Figure imgf000089_0001
Figure imgf000089_0002
Figure imgf000089_0003
Figure imgf000089_0004
Figure imgf000089_0005
J=9.0, 2.5Hz), 7.27 (m, 2H), 7.15 (dd, IH, J=9.0, 4.3Hz), 7.09-7.04 (m, 3H), 5.97 (m, IH), 1.93 (d, 3H, J=I.2Hz) and 1.90 (d, 3H, J=I .6Hz).
Figure imgf000090_0001
Figure imgf000090_0002
Figure imgf000090_0003
Figure imgf000090_0004
E 225 2-(3,5-dimethylisoxazol-4-yl)-4,5-difluoro-l-(4-hydroxyphenyl)-lH-indole-3-carbonitrile
R = 3,5-dimethyl-isoxazol-4-yl RZ = CN
Figure imgf000091_0001
Figure imgf000091_0003
Figure imgf000091_0004
Figure imgf000091_0005
Examples 229-232
Examples 229-232 below were prepared according to General Method 2 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 229-232 are described in Examples 2-4, 8, 16, 24 and 38-40 above.
Figure imgf000091_0002
Figure imgf000092_0001
Figure imgf000092_0002
Figure imgf000092_0003
Figure imgf000092_0004
Examples 233-270
Examples 233-270 below were prepared according to General Method 1 above. Full experimental details of the individual steps of that general method applicable for the synthesis of Examples 233-270 are described in Examples 1-4, 10-11, 14-16, 38-40 and 45 above.
Figure imgf000093_0001
Figure imgf000093_0002
Figure imgf000093_0003
Figure imgf000093_0004
Figure imgf000093_0005
Figure imgf000094_0001
Figure imgf000094_0002
Figure imgf000094_0003
Figure imgf000094_0004
Figure imgf000094_0005
E 241 (Z)-l-(2,3-difluoro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-N'-hydroxy-lH-indole-3-
Figure imgf000095_0001
Figure imgf000095_0002
Figure imgf000095_0003
Figure imgf000095_0004
Figure imgf000095_0005
ES/MS nVz: 333.2 (pos. M + H), 331.3 (neg. M - H); 1H NMR (acetone-d6, 500MHz): 1H NMR (acetone-d6, 500MHz): 7.87 (m, IH), 7.48 (d, IH, J=2.2Hz), 7.42-7.37 (m, 2H), 7.20 (m, IH), 7.15 (t, IH, J=8.6Hz), 6.69 (m, IH), 6.60 (dd, IH, J=2.5, 11. OHz), 6.22 (d, IH, J=2.2Hz) and 3.87 (s, 3H).
Figure imgf000096_0001
Figure imgf000096_0002
Figure imgf000096_0003
Figure imgf000096_0004
Figure imgf000097_0001
Figure imgf000097_0002
Figure imgf000097_0003
Figure imgf000097_0004
E 254 (Z)-4-fluoro-l-(2-fluoiO-4-hydroxyphenyl)-N'-hydroxy-2-(3-methylthiophen-2-yl)-lH-indole-
Figure imgf000098_0001
Figure imgf000098_0002
Figure imgf000098_0003
Figure imgf000098_0004
E 258 (Z)-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(l,3,5-trimethyl-lH-pyrazol-4-yl)-lH-indole- 3-carboximidamide
Figure imgf000099_0001
Figure imgf000099_0002
Figure imgf000099_0003
Figure imgf000099_0004
Figure imgf000099_0005
E 263 2-(3, 5 -dimethylisoxazol-4-yl)- 1 -(3-fluoro-4-hydroxyphenyl)-lH-indole-3-carboximidamide
Figure imgf000100_0001
Figure imgf000100_0002
Figure imgf000100_0003
Figure imgf000100_0004
Figure imgf000100_0005
Figure imgf000101_0002
Figure imgf000101_0003
Figure imgf000101_0004
Example 271
Example 271 below was prepared according to General Method 3 above. Full experimental details of the individual steps of that general method are described in Examples 2-4, 8, 16, 24 and 38-40 above.
Figure imgf000101_0001
E 271 (Z)-2-(3,5-dimethylisoxazol-4-yl)-6-fluoro-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3- carboximidamide
Figure imgf000102_0001
Binding Assay 1: Estrogen Receptor Binding Assay
The estrogen receptor ligand binding assays are designed as scintillation proximity assays (SPA), employing the use of tritiated estradiol (3H-E2) and recombinant expressed biotinylated estrogen receptor binding domains. The binding domains of human ERa (ERa-LBD, pET-N-AT #1, aa 301-595) and ERβ (ERβ-LBD, pET-N-AT #1, aa 255-530) proteins are produced in E.coli ((BL21, (DE3), pBirA)) at 22 C in 2xLB medium supplemented with 50 uM biotin. After 3 h of IPTG induction (0.55 mM), cells are harvested by centrifugation at 7300xg for 15 min and cell pellets stored frozen in -20C. Extraction of ERa and ERβ are performed using 5 g of cells suspended in 50 mL of extraction buffer (50 mM Tris, pH 8.0, 100 mM KCl, 4 mM EDTA, 4 mM DDT and 0.1 mM PMSF). The cell suspension is run twice through a Microfluidizer M-11OL (Microfluidics) and centrifuged at 15,000xg for 60 min. The supernatant is aliquoted and stored in -70C.
Dilute ERα-LBD or ERβ-LBD extracts in assay buffer (18 mM K2HPO4, 2 mM KH2PO4, 20 mM Na5MoO4, 1 mM EDTA, ImM TCEP) 1 :676 and 1 :517 for alpha and beta respectively. The diluted receptor concentrations should be 900 fmol/L. Preincubate the extracts with streptavidin coated polyvinyltoluene SPA beads (RPNQ0007, GE Healthcare) at a concentration of 0.43 mg/mL for lhr at room temperature.
Test compounds are evaluated over a range of concentrations from 157 μM to 37.5 pM. The test compound stock solutions should be made in 100% DMSO at 5x of the final concentration desired for testing in the assay. The amount of DMSO in the test wells of the 384 well plate will be 20%. Add 18μl aliquots of test compounds to the assay plates followed by 35μl of the preincubated receptor/SPA bead mix and finally add 35μl of 3nM 3H-E2. Cover the plates with a plastic sealer, centrifuge for 1 minute at 1000 rpm and equilibrate over night on a shaker at room temperature. The following morning, centrifuge the plates 5 minutes at 2000 rpm and measure on a plate scintillation counter e.g. a PerkinElmer Microbeta 1450 Trilux.
For compounds able to displace 3[H]-E2 from the receptor an IC50-value (the concentration required to inhibit 50% of the binding of 3[H]-E2) is determined by a non-linear four parameter logistic model; b = ((bmax-bmin)/(l+(I/IC5o)S))+bmin I is added concentration of binding inhibitor, IC50 is the concentration of inhibitor at half maximal binding and S is a slope factor. The Microbeta-instrument generates the mean cpm (counts per minute) value / minute and corrects for individual variations between the detectors thus generating corrected cpm values.
Binding Assay 2: Estrogen Receptor Filter Binding Assay
The ligand binding domain of the human estrogen receptor beta (hERβ-LBD) is used in a competition binding assay with filter separation of bound and free ligand. The assay utilizes tritiated estradiol (3H-E2) as beta particle emitting tracer and recombinant expressed human estrogen beta receptor binding domain. The binding domain of human ERβ (hERβ-LBD, pET-N-AT #1, aa 255-530) protein is produced in Escherichia coli ((BL21, (DE3), pBirA)) at 22°C in 2xLB medium supplemented with 50 μM biotin. After 3 h of isopropyl β-D-1-thiogalactopyranoside induction (0.55 mM), cells are harvested by centrifugation at 7300xg for 15 min and cell pellets stored frozen in -20°C. Extraction of hERβ-LBD is performed using 5 g of cells suspended in 50 mL of extraction buffer (50 mM Tris, pH 8.0, 100 mM KCl, 4 mM ethylenediaminetetraacetic acid (EDTA), 4 mM dithiothreitol and 0.1 mM phenylmethanesulfonyl fluoride (TCEP). The cell suspension is run twice through a Microfluidizer M-11OL (Microfluidics) and centrifuged at 15,000xg for 60 min. The supernatant is aliquoted and stored in -700C. Estrogen receptor extract is diluted 1 :400 in assay buffer (18 mM K2HPO4, 2 mM KH2PO4, 20 mM Na2MoO4, 1 mM EDTA, ImM TCEP, pH 8.0). Test compounds are evaluated over a range of concentrations from 2 μM to 10 pM. The test compound stock solutions should be made in 100% dimethyl sulfoxide (DMSO) at 51x of the final concentration desired for testing in the assay. The final fraction of DMSO in the wells of the 96 well assay plate will be 2%. lOOμl 3H-E2 is added to the assay plates followed by 4μl aliquots of test compounds and lOOμl of the diluted receptor extract. The assay plates are stored over night at +4°C. Receptor bound and free tracer are separated over a glass fiber filter (FILTERMAT B, PerkinElmer)) on a cell harvester (TOMTECMACH3, Tomtec) with wash buffer (18mM K2HPO4, 2mM KH2PO4, 0.5mM EDTA). The filters are dried at 600C for 1 hour and then merged by heat with a scintillating wax (MELTILEX, PerkinElmer) before measuring on a plate beta counter (Wallac Microbeta Trilux 1450- 028, PerkinElmer). The Trilux-instrument generates mean counts per minute (cpm) and corrects for individual variations between the detectors, thus generating corrected cpm values (ccpm). The IC50 values, defined as the midpoint between maximum binding and minimum binding on the sigmoid binding curve, are calculated with XLfit software version 2.0 or later (IDBS) with a four parameter logistic model; b = ((bmax-bmin)/(l +(1/IC50) S))+bmin where I is added concentration of binding inhibitor, IC50 is the concentration of inhibitor at half maximal binding and S is a slope factor, Transactivation Assay 1 : Transactivation assay in human embryonic kidney 293 cells stably transfected with pERE-ALP and human estrogen receptor alpha
The expression vector pMThERα contains an insert of wild type human estrogen receptor alpha with deleted leader. The pERE-ALP reporter construct contains the gene for the secreted form of placental alkaline phosphatase (ALP) and the vitellogenin estrogen response element (ERE). The human embryonic kidney 293 cells are transfected in two steps. Firstly, a stable clone mix transfected with the pERE-ALP reporter gene construct and pSV2-Neo for selection is developed. Secondly, the stable clone mix is transfected with pMThERα and a pKSV-Hyg resistance vector for selection. All transfections are performed using Lipofectamine (Invitrogen) according to supplier's recommendations. A selected clone with both pERE-ALP and pMThERα is used for the transactivation assay.
The cells are seeded in 384-well plates at 12 500 cells per well in Ham's F12 Coon's modification (without phenol red) with 10 % dextran-coated charcoal treated (DCC) fetal bovine serum (FBS), 2 niM L-glutamine and 50 μg/ml gentamicin. After 24 h incubation (37°C, 5 % CO2) the seeding medium is discarded and replaced with 20 μl Ham's F12 Coon's modification (without phenol red) with 1.5 % DCC- FCS, 2 mM L-glutamine and supplemented with 100 U/ml penicillin and 100 μg/ml streptomycin. The selected compounds are added to the wells in 12 concentrations ranging from 3.3 pM to 33 μM. The compounds are dissolved in 100 % dimethylsulphoxide (DMSO) and the final concentration of DMSO in the assay is 0.1 %. After 72 h incubation (37°C, 5 % CO2) the medium is assayed for ALP activity by a chemiluminescence assay; a 10 μl aliquot of the cell culture medium is mixed with 100 μl assay buffer (0.1 M diethanolamine, 1 mM MgCl2) and 0.5 mM disodium 3-(4-methoxyspiro l,2-dioxetane-3,2'-(5'- chloro)-tricyclo[3.3.1.13,7]decan-4-yl)phenyl phosphate (CSPD) (Tropix, Applied Biosystems) and incubated for 20 min at 37°C and 15 min at room temperature before measurement chemiluminescent light signal (one second per well) in a Wallac Microbeta Trilux 1450-028 (PerkinElmer). The half maximal effective concentrations (EC50) are calculated from the curves fitted to the concentration- response data with a four parameter logistic model in XLfit software version 2.0 (IDBS) or later.
Transactivation Assay 2: Transactivation assay in human embryonic kidney 293 cells stably transfected with pERE2-ALP and human estrogen receptor beta
Generation of stable HEK293 cell lines (CRL-1573; American Type Culture Collection) expressing the reporter vector pERE2-ALP and human estrogen receptor beta (hERβ 530) have been described (MoI Pharmacol 1998, 54,105-112; Endocrinology 2002, 143, 1558-1561).
The cells were seeded in 384-well plates at 12 500 cells per well in Ham's F12 Coon's modification
(without phenol red) with 10 % dextran-coated charcoal treated (DCC) fetal bovine serum (FBS), 2 mM L-glutamine and 50 μg/ml gentamicin. After 24 h incubation (37°C, 5 % CO2) the seeding medium was discarded and replaced with 20 μl Ham's Fl 2 Coon's modification (without phenol red) with 1.5 % DCC- FCS, 2 mM L-glutamine and supplemented with 100 U/ml penicillin and 100 μg/ml streptomycin. The selected compounds were added to the wells in 12 concentrations ranging from 3.3 pM to 33 μM. The compounds were dissolved in 100 % dimethylsulfoxide (DMSO) and the final concentration of DMSO in the assay was 0.1 %. After 72 h incubation (370C, 5 % CO2) the medium was assayed for ALP activity by a chemiluminescence assay; a 10 μl aliquot of the conditioned medium was mixed with 100 μl assay buffer (0.1 M diethanolamine, 1 mM MgC12) and 0.5 mM disodium 3-(4-methoxyspiro 1,2-dioxetane- 3,2'-(5'-chloro)-tricyclo[3.3.1.13,7]decan-4-yl)phenyl phosphate (CSPD) (Tropix, Applied Biosystems) and incubated for 20 min at 37°C and 15 min at room temperature before measurement of the chemiluminescent signal (one second per well) in a Wallac Microbeta Trilux 1450-028 (PerkinElmer). The ALP activity expressed in LCPS is directly proportional to the level of ALP expressed by the cells. The half maximal effective concentrations of the test compounds (EC50) were calculated from the curves fitted to the concentration-response data with a four parameter logistic model in XLfit software version 2.0 (IDBS) or later.
The compounds of Examples 1-271 exhibit one or more of the following:
(i) a binding affinity to the estrogen receptor α-subtype in the range of IC50 1 to 10,000 nM in binding assay 1; (ii) a binding affinity to the estrogen receptor β-subtype in the range of IC50 1 to 10,000 nM in binding assay 1;
(iii) a binding affinity to the estrogen receptor α-subtype in the range Of IC50 1 to 10,000 nM in binding assay 2;
(iv) a binding affinity to the estrogen receptor β-subtype in the range of IC50 1 to 10,000 nM in binding assay 2;
(v) a potency in the range of EC50 1 to 10,000 nM at the estrogen receptor α-subtype in transactivation assay 1 ;
(vi) a potency in the range of EC50 1 to 10,000 nM at the estrogen receptor β-subtype in transactivation assay 2.
Preferred Example compounds of the invention are those which exhibit a binding affinity to the estrogen receptor β-subtype at lower concentrations within the IC5O range shown above. For example, the compounds of Examples 1, 2, 4-6, 11, 23, 39, 42, 43, 46, 49-51 , 53, 54, 63, 64, 68, 70, 83, 86, 87, 95,
100, 101, 107, 110, 125, 126, 128, 129, 132-141, 143, 144, 146, 147, 158, 163, 188, 191 , 194 , 212-217, 219-221, 223, 224, 235, 243, exhibit a binding affinity to the estrogen receptor β-subtype in the range of
IC50 1 to 200 nM in binding assay 1. Preferred Example compounds of the invention are those which are selective for the estrogen receptor β- subtype over the estrogen receptor α-subtype in binding assay 1. For example, the compounds of Examples 2, 4, 5, 11, 23, 43, 51, 53, 68, 70, 39, 95, 133, 134, 143, 146,188, 191, 213, 215, 221, 224, 235, display selectivity for the estrogen receptor β-subtype of 50 or greater in the binding assay.
Preferred Example compounds of the invention are those which display a potency at the estrogen receptor β-subtype at lower concentrations within the EC50 range shown above. For example, the compounds of Examples 11, 14, 39, 41, 42, 47, 68, 163, 176,187,188, 191, 194, 220, 221, 223, 235-238, 255, 266, 268, 269, 271 exhibit a potency in the range OfEC50 1 to 50 nM at the estrogen receptor β-subtype in transactivation assay 2.
Preferred Example compounds of the invention are those which are selective for the estrogen receptor β- subtype over the estrogen receptor α-subtype in the transactivation assays 1 and 2. For example, the compounds of Examples 11, 14, 41, 42, 44, 221, 235-238, 241, 255, 260, 268, 271 display selectivity for the estrogen receptor β-subtype of 50 or greater in the transactivation assays.
Particularly preferred Example compounds of the invention are those which exhibit both a binding affinity to the estrogen receptor β-subtype at lower concentrations within the IC50 range shown above and a potency at the estrogen receptor β-subtype at lower concentrations within the EC50 range shown above. For example, the compounds of Examples 11, 39, 42, 68, 163, 188, 191 , 194, 220, 221, 223, 235 exhibit a binding affinity to the estrogen receptor β-subtype in the range of IC50 1 to 200 nM in binding assay 1 and a potency in the range of EC50 1 to 50 nM at the estrogen receptor β-subtype in transactivation assay 2.

Claims

Claims
1. A compound of formula (I) or a pharmaceutically acceptable ester, amide, carbamate, solvate or salt thereof, including a salt of such an ester, amide or carbamate, and a solvate of such an ester, amide, carbamate or salt,
Figure imgf000107_0001
(I)
wherein R1 is selected from the group consisting of halogen, cyano, nitro, ORA, N(RB)2, -C(O)C1^aIkVl, -SO2Ci_4alkyl, Ci_6alkyl, C2-6alkenyl, C2.6alkynyl, haloC^alkyl, dihaloC,_6alkyl, trihaloCi_6alkyl, haloC2_ 6alkenyl, dihaloC2_6alkenyl, trihaloC2_6alkenyl, cyanoC^alkyl, Ci_4alkoxyC[_6 alkyl, C3.8cycloalkyl, C3_8cycloalkylCi_6 alkyl, phenyl, benzyl, and 5-10 membered heterocyclyl, wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents, each substituent being selected from the group consisting of ORA, halogen, cyano, nitro, -C(O)Ci_4alkyl, Cj. βalkyl, C2.6alkenyl, C2.6alkynyl, haloCi.6 alkyl, dihaloC|_6alkyl and trihaloCi.6alkyl;
R2 is selected from the group consisting of halogen, cyano, nitro, ORA, N(RB)2, N(OH)2, -C(O)C i_4alkyl optionally substituted with from 1 to 3 halogens, -SO2C|.4alkyl, -C(O)NH-OH, -C(NH2)=N-0H, -C(CO2H)=N-OH, -C(NH2)=NH, -C(NH Ci.4alkyl)=NH, -C(O-C,_4alkyl)=NH, -C(NH2)=N-NH2, -NH-C(NH2)=NH, -NH-C(O)NH2, -N=C(-NH-CH2CH2-NH-), -S-CN, -S-C(NH2)=NH, -S-C(NH2)=N- OH, -CO2H, -CH2-CO2H, -CH(OH)CO2H, -C(O)CO2H, SO3H, CH2SO3H, C,.6alkyl, haloC,.6alkyl, dihaloCi.6alkyl, trihaloCi.6alkyl, cyanoC|.6alkyl, Ci_4alkoxyCi.6 alkyl, C2_6alkenyl, C2.6alkynyl, C3. gcycloalkyl, C3_8cycloalkylC)_6 alkyl, phenyl, benzyl and 5-10 membered heterocyclyl wherein said phenyl, benzyl or heterocyclyl group can be either unsubstituted or substituted with from 1 to 3 substituents each substituent being selected from the group consisting of 0RΛ, halogen, cyano, nitro,
Ci_6alkyl, C2.6alkenyl, C2_6alkynyl, haloCi.6alkyl, dihaloC|_6alkyl and trihaloCi_6alkyl; provided that if one of R1 and R2 represents halogen', the other must represent a group other than halogen;
each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen, 0RΛ, halogen, cyano, nitro, C^alkyl, C2.6alkenyl, C2_6alkynyl, haloCi.calkyl, dih'aloCi_6alkyl and trihaloCi_6alkyl; each RΛ is independently selected from the group consisting of hydrogen, C^alkyl, C2_6alkenyl, C2. 6alkynyl, C3_8cycloalkyl, C3_8cycloalkylCi_6alkyl, C6-ioaryl and C6-I0 arylCi-6alkyl, each optionally substituted by from 1 to 3 halogen atoms; and
each RB is independently selected from the group consisting of hydrogen, C^alkyl, C2_6alkenyl, C2. βalkynyl, C3-8cycloalkyl,
Figure imgf000108_0001
Cβ-ioaryl and C6_io
Figure imgf000108_0002
each optionally substituted by from 1 to 3 halogen atoms;
with the proviso that the compound of formula (I) is not 4-[3-(4,5-Dihydro-lH-imidazol-2-yl)-2-(3,5-dimethyl-isoxazol-4-yl)-indol-l -yl]-phenol; l-(4-Hydroxy-phenyl)-2-(4-methyl-imidazol-l-yl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-(lH-pyrazol-3-yl)-lH-indole-3-carbonitrile;
1 -(3 -Chloro-4-hydroxy-phenyl)-2-( 1 -methyl- 1 H-pyrazol-4-yl)- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-prop-l-ynyl-lH-indole-3-carboxylic acid amide; or l-(4-Hydroxy-phenyl)-2-thiazol-2-yl-lH-indole-3-carboxylic acid.
2. A compoound as claimed in claim 1, in which each RΛ is independently selected from the group consisting of hydrogen, Ci.4alkyl, C2-4alkenyl, C2_4alkynyl, C3_6cycloalkyl, phenyl and benzyl.
3. A compound as claimed in claim 2, in which each RΛ independently represents hydrogen or C|.4alkyl.
4. A compound as claimed in any one of the preceding claims, in which each RB is independently selected from the group consisting of hydrogen and Cμ4alkyl.
5. A compound as claimed in any one of the preceding claims, in which R1 is selected from the group consisting of ORA, N(RB)2, -C(O)C Malkyl, C,.6alkyl, C2.f,alkenyl, C2.5alkynyl, haloCMalkyl, dihaloC,. 4alkyl, trihaloC^alkyl, haloC2.4alkenyl, dihaloC2.4alkenyl, trihaloC2-4alkenyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted by 1 to 3 substituents selected from the group consisting of ORA, halogen, cyano, -C(O)C i_4alkyl, C|.4alkyl, C2. 4alkenyl, C2_4alkynyl, haloCi_4alkyl, dihaloCi_4alkyl and trihaloCi_4alkyl.
6. A compound as claimed in claim 5, in which R1 is selected from the group consisting of ORΛ, N(RB)2, -C(O)C i_4alkyl, C^alkyl, C2_4alkenyl, C2_4alkynyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted by 1 to 3 substituents selected from halogen, cyano, CMalkyl, -C(O)C ι_4alkyl, and ORA in which RA represent hydrogen or C|.4alkyl.
7. A compound as claimed in any one of the preceding claims, in which R2 is selected from the group consisting of halogen, ORA, N(RB)2, -C(O)Ci_4alkyl optionally substituted with from 1 to 3 halogens, -C(NH2)=N-0H, -CO2H, -CH2-CO2H, C^alkyl, C2-6alkenyl, C2.6alkynyl, haloC1.4alkyl, dihaloC,.4alkyl, trihaloCMalkyl, haloC2_4alkenyl, dihaloC2.4alkenyl, trihaloC2.4alkenyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted by 1 to 3 substituents selected from the group consisting of 0RΛ, halogen, cyano, -C(O)Ci_4alkyl, Ci_4alkyl, C2_ 4alkenyl, C2.4alkynyl, haloC^alkyl, dihaloCi.4alkyl and trihaloC].4alkyl.
8. A compound as claimed in claim 7, in which R2 is selected from the group consisting of -C(O)Ci_ 4alkyl, -C(NH2)=N-0H, -CO2H, -CH2-CO2H, CMalkyl, C2.4alkenyl, C2.4alkynyl, and 5-6 membered heterocyclyl wherein said heterocyclyl group can either be unsubstituted or substituted by 1 to 3 substituents selected from halogen, cyano, Ci_4alkyl, -C(O)Ci_4alkyl, and 0RA.
9. A compound as claimed in any one of the preceding claims, in which each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen, 0RA, halogen, cyano, Ci_4alkyl, haloCi.4alkyl, dihaloCi_4alkyl, and trihaloC).4alkyl.
10. A compound as claimed in claim 9, in which each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen, OH, halogen, cyano, methyl, or trifluoromethyl.
11. A compound as claimed in claim 1, in which R1 is selected from the group consisting of 0RA, N(R3)- 2, -C(O)CMalkyl, Ci_6alkyl, C2.6alkenyl, C2.6alkynyl, haloCi.4alkyl, dihaloCi_4alkyl, trihaloC|.4alkyl, haloC2.4alkenyl, dihaloC2.4alkenyl, trihaloC2-4alkenyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group may be either unsubstituted or substituted as above by 1 to 3 substituents selected from the group consisting of 0RA, halogen, cyano, -C(O)Ci_4alkyl, Ci_4alkyl, C2.
4alkenyl, C2.4alkynyl, haloCi.4alkyl, dihaloC|.4alkyl and trihaloC).4alkyl;
R2 is selected from the group consisting of halogen, 0RA, N(RB)2, -C(O)Ci_4alkyl optionally substituted with from 1 to 3 halogens, -C(NH2)=N-0H, -CO2H, -CH2-CO2H, C,.6alkyl, C^alkenyl, C2_6alkynyl, haloCi-4alkyl, dihaloCMalkyl, trihaloCi_4alkyl, haloC|_4alkenyl, dihaloCi.4alkenyl, trihaloC|.4alkenyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted by 1 to 3 substituents selected from the group consisting of 0RA, halogen, cyano, -C(O)C1 _4alkyl, Ci_4alkyl, C2-4alkenyl, C2.4alkynyl, haloCi.4alkyl, dihaloCi_4alkyl and trihaloC|.
4alkyl; each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen, 0RA, halogen, cyano, C|.4alkyl, haloC,.4alkyl, dihaloC1.4alkyl, and trihaloC1.4alkyl; each RA is independently selected from the group consisting of hydrogen, Ci_4alkyl, C2.4alkenyl, C2.
4alkynyl, C3_6cycloalkyl, phenyl and benzyl; and each RB is independently selected from the group consisting of hydrogen and
Figure imgf000110_0001
12. A compound as claimed in claim 11 , in which R1 is selected from the group consisting of ORA, N(RB)2, -C(O)Ci_4alkyl,
Figure imgf000110_0002
C2-4alkenyl, C2-4alkynyl, phenyl, and 5-6 membered heterocyclyl, wherein said phenyl or heterocyclyl group can either be unsubstituted or substituted by 1 to 3 substituents selected from halogen, cyano, Ci_4alkyl, -C(O)CMalkyl, and ORA; each RA independently represents hydrogen or CMalkyl; and each RB is independently selected from the group consisting of hydrogen and d^alkyl.
13. A compound as claimed in either claim 11 or claim 12, in which R2 is selected from the group consisting of -C(O)C)-4alkyl optionally substituted with from 1 to 3 halogens, -C(NH2)=N-OH, -CO2H, -CH2-CO2H,
Figure imgf000110_0003
C2_4alkenyl, C2_4alkynyl, and 5-6 membered heterocyclyl wherein said heterocyclyl group can be either unsubstituted or substituted by 1 to 3 substituents selected from the group consisting of halogen, cyano, -C(O)Ci.4alkyl, Ci.4alkyl, C2_4alkenyl, C2.4alkynyl, haloCi_4alkyl,
Figure imgf000110_0004
and trihaloCi_4alkyl, and ORA, in which RA represents hydrogen or Chalky!.
14. A compound as claimed in any one of claims 11 to 13, in which R2 is selected from the group consisting of -C(O)CH3, -C(NH2)=N-0H, -CO2H1 -CH2-CO2H, CMalkyl, C2.4alkenyl, C2.4alkynyl, and 5- 6 membered heterocyclyl wherein said heterocyclyl group can be either unsubstituted or substituted 1 to 3 substituents selected from halogen, cyano, Ci_4alkyl, -C(O)C |.4alkyl, and 0RA in which RA represents hydrogen or Ci.4alkyl.
15. A compound as claimed in any one of claims 11 to 14, in which each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen, OH, halogen, cyano, methyl, or trifluoromethyl.
16. A compound as claimed in claim 1, in which R1 is a 5-6 membered heterocyclyl group, wherein said heterocyclyl group is substituted with from 1 to 3 substituents selected from halogen, cyano and C|.4alkyl; R2 is selected from the group consisting of -C(O)CH3, -C(NH2)=N-0H, -CO2H, and -CH2-CO2H; and each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen and halogen.
17. A compound as claimed in claim 16, in which R1 is a 5-membered heterocyclyl group, wherein said heterocyclyl group is substituted with two substituents independently selected from methyl and ethyl; R2 is -C(NH2)=N-0H; and each of R3, R4, R5 and R6 is independently selected from the group consisting of hydrogen and halogen.
18. A compound as claimed in claim 1, which is any one of the following compounds: 2-Bromo- 1 -(4-hydroxy-phenyl)- 1 H-indole-3-carbonitrile;
1 -(4-Hydroxy-phenyl)-2-thiophen-3 -yl- 1 H-indole-3 -carbonitrile;
2-(3 -Cy ano-fur an-2-yl)- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
1 -(4-Hydroxy-phenyl)-2 -pyrrol- 1 -yl-1 H-indole-3 -carbonitrile; 2-Dimethylamino-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
1 -(4-Hydroxy-phenyl)-2-isoρropyl- 1 H-indole-3 -carbonitrile;
2-Acetyl-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid;
1 -[I -(4-Hydroxy-phenyl)-2-phenyl-lH-indol-3-yl]-ethanone; 1 -(4-Hydroxy-phenyl)-2 -phenyl- 1 H-indole-3 -carboxylic acid, amide;
(Z)-2-(3,5-dimethylisoxazol-4-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide;
[2-(3 ,5 -Dimethyl-isoxazol-4-yl)-l -(4-hydroxy-phenyl)- 1 H-indol-3 -yl] -carbamic acid tert-butyl ester;
4-[3-Amino-2-(3,5-dimethyl-isoxazol-4-yl)-indol-l-yl]-phenol;
(Z)-2-(3 ,5-dimethylisoxazol-4-yl)-7-fluoro-N'-hydroxy- 1 -(4-hydroxyphenyl)- 1 H-indole-3 - carboximidamide;
(Z)-2-(5-chlorothiophen-2-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide;
1 -(2,3 -Difluoro-4-hydroxy-phenyl)-2-(3 ,5 -dimethyl-isoxazol-4-yl)- 1 H-indole-3 -carbonitrile;
2-(3 ,5 -dimethylisoxazol-4-yl)- 1 -(4-hydroxyphenyl)- 1 H-indole-3 -carbohydrazonamide;
4-(2-(3,5-dimethylisoxazol-4-yl)-3-(l,2,4-oxadiazol-3-yl)-lH-indol-l-yl)phenol; 2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid, methyl ester;
2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid, hydroxyamide;
4-[2-(3,5-Dimethyl-isoxazol-4-yl)-3-methanesulfonyl-indol-l-yl]-phenol; l-[2-(3,5-Dimethyl-isoxazo]-4-yl)-l-(4-hydroxy-phenyl)-lH-indol-3-yl]-2,2,2-trifluoro-ethanone;
4-(3-bromo-2-(3,5-dimethylisoxazol-4-yl)-lH-indol-l-yl)phenol; 2-Bromo-5 -fluoro- 1 -(4-hydroxy-phenyl)- 1 H-indole-3-carbonitrile;
(Z)-2-(4-fluorophenoxy)-N'-hydroxy-l-(4-hydiOxyphenyl)-lH-indole-3-carboximidamide; 4-(2-(3,5-dimethylisoxazol-4-yl)-3-nitro-lH-indol-l-yl)phenol; 4-(3-(dihydroxyamino)-2-(3 ,5-dimethylisoxazol-4-yl)- 1 H-indol- 1 -yl)phenol; N-(2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)acetamide N-(2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)methanesulfonamide; 1 -(2-(3 ,5 -dimethylisoxazol-4-yl)- 1 -(4-hydroxyphenyl)- 1 H-indol-3 -yl)urea; 4-(2-(3 ,5 -dimethylisoxazol-4-yl)-3 -thiocyanato- 1 H-indol- 1 -yl)phenol;
(E)-2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl N'-hydroxycarbamimidothioate; 4-(3 -benzyl -2 -phenyl- 1 H-indol- 1 -yl)phenol; 2-(2-(3 ,5 -dimethylisoxazol-4-yl)- 1 -(4-hydroxyphenyl)- 1 H-indol-3 -yl)-2-oxoacetamide;
(Z)-2-(2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2-(hydroxyimino)acetamide; 2-(2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indol-3-yl)-2-hydroxyacetamide; 2-(2-(3 ,5 -dimethylisoxazol-4-yl) - 1 -(4-hydroxyphenyl)- 1 H-indol-3 -yl)acetamide;
2-((Z)-But- 1 -enyl)- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-(2-methyl-prop-l-enyl)-lH-indole-3-carbonitrile;
1 -(2,3 -Difluoro-4-hydroxy-phenyl)-2-(2-methyl-allyl)- 1 H-indole-3 -carbonitrile; (Z)-2-(5-ethyl-3-methylisoxazol-4-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboxiniidamide;
4-(2-(3 ,5 -dimethylisoxazol-4-yl) -3 -phenyl-1 H-indol- 1 -yl)phenol;
4-(3 -chloro-2-(3 ,5 -dimethylisoxazol-4-yl)- 1 H-indol- 1 -yl)phenol;
2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-sulfonamide;
2-(3,5-dimethylisoxazol-4-yl)-l-(2-fluoro-4-hydroxyphenyl)-lH-indole-3-carboximidamide; 1 -(4-Hydroxy-phenyl)-2 -phenyl- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-methyl-lH-indole-3-carbonitrile;
2-(3-Cyano-thiophen-2-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
1 -(4-Hydroxy-phenyl)-2-((E)-propenyl)- 1 H-indole-3 -carbonitrile;
1 -(4-Hydroxy-phenyl)-2-thioρhen-2-yl- 1 H-indole-3 -carbonitrile; 2-(3,5-Dimethyl-isoxazol-4-yl)-l -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-pyridin-4-yl-lH-indole-3-carbonitrile;
1 -(4-Hydroxy-phenyl)-2-( 1 -methyl- lH-pyrrol-2-yl)- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-(3-methyl-thiophen-2-yl)-lH-indole-3-carbonitrile;
1 -(4-Hydroxy-phenyl)-2-isopropylamino- 1 H-indole-3 -carbonitrile; 2-Ethylamino-l -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
2-Butylamino- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
1 -(4-Hydroxy-phenyl)-2-piperidin- 1 -yl- 1 H-indole-3 -carbonitrile;
1 -(4-Hydroxy-phenyl)-2-pyrrolidin- 1 -yl- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-morpholin-4-yl-lH-indole-3-carbonitrile; 2-Diethylamino-l-(4-hydroxy-phenyl)-lH-indole-3-carbom'trile;
2-Ethynyl- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-vinyl-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-lH-indole-2,3-dicarbonitrile;
1 -(4-Hydroxy-phenyl)-2 -prop- 1 -ynyl-1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-pyridin-2-yl-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-(2-methyl-allyl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-((Z)-propenyl)-lH-indole-3-carbonitrile;
2-(Butyl-methyl-amino)-l -(4-hydroxy-phenyl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-((Z)-l-methyl-propenyl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-imidazol-l -yl-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-[l,2,4]triazol-l-yl-lH-indole-3-carbonitrile;
2-(3,5-Dimethyl-pyrazol-l -yl)-l-(4-hydroxy-phenyl)-.lH-indole-3-carbonitrile; 1 -(4-Hydroxy-phenyl)-2-pyrazol-l -yl-1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-(5-methyl-imidazol-l-yl)-lH-indole-3-carbonitrile;
1 -(4-Hydroxy-phenyl)-2-(5 -methyl-pyrazol- 1 -yl)- 1 H-indole-3 -carbonitrile ;
1 -(4-Hydroxy-phenyl)-2-(3 -methyl-pyrazol- 1 -yl)- 1 H-indole-3 -carbonitrile; 1 -(4-Hydroxy-phenyl)-2-thiazol-2-yl- 1 H-indole-3 -carbonitrile;
1 -(4-Hydroxy-phenyl)-2-(2-methoxy-thiazol-4-yl)- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-thiazol-4-yl-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-(3-methyl-but-2-enyl)-lH-indole-3-carbonitrile;
2-((E)-But- 1 -enyl)- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-(5-methyl-thiophen-2-yl)-lH-mdole-3-carbonitrile;
2-(5-Acetyl-thiophen-2-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-(l-methyl-lH-pyrazol-4-yl)-lH-indole-3-carbonitrile;
2-(5 -Chloro-thiophen-2-yl)- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile; l-(4-Hydroxy-phenyl)-2-(4-methyl-thiophen-3-yl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-(4-methyl-thiophen-2-yl)-lH-indole-3-carbonitrile;
2-(4-Cyano-thiophen-3-yl)-l-(4-hydroxy-ρhenyl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-(2-methyl-2H-pyrazol-3-yl)-lH-indole-3-carbonitrile;
1 -(4-Hydroxy-phenyl)-2-(l ,3 ,5 -trimethyl- 1 H-pyrazol-4-yl)- 1 H-indole-3 -carbonitrile;
2-(2-Acetyl-pyrrol- 1 -yl)- 1 -(4-hydroxy-phenyl)-l H-indole-3 -carbonitrile; 2-(2-Ethyl-pyrrol-l -yl)-l -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
2-(2-Cyano-pyrrol-l-yl)-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile; l-(4-Hydroxy-phenyl)-2-(2-methyl-pyrrol-l-yl)-lH-indole-3-carbonitrile; l-(3-Chloro-5-lluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile;
1 -(3 -Chloro-5 -fluoro-4-hydroxy-phenyl)-2-(3-cyano-thioρhen-2-yl)- 1 H-indole-3 -carbonitrile; 1 -(3 -Chloro-5 -fluoro-4-hydroxy-phenyl)-2-(3 -cyano-furan-2-yl)- 1 H-indole-3 -carbonitrile;
2-Bromo-l-(3-chloro-5-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
2-Bromo-l-(2-fluoro-4-hydroxy-phenyl)-l H-indole-3 -carbonitrile; l-(2-Fluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile;
2-Bromo-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile; 2-Bromo-l-(2,3-difluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
2-Bromo-l-(2,5-difluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
2-Bromo-l-(3-chloro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
2-Bromo-l-(3,5-difluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile; l-(3-Fluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile; l-(3,5-Difluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile; l-(3-Chloro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile; l-(2,5-Difluoro-4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile; l-(3,5-Difluoro-4-hydroxy-phenyl)-2-thiophen-3-yl-lH-indole-3-carbonitrile;
1 -(3 ,5 -Difluoro-4-hydroxy-phenyl)-2-thiophen-2-yl- 1 H-indole-3 -carbonitrile; l-(3,5-Difluoro-4-hydroxy-phenyl)-2-(3,5-dimethyl-isoxazol-4-yl)-lH-indole-3-carbonitrile; 1 -(3,5-Difluoro-4-hydroxy-phenyl)-2-(l -methyl- lH-pyrrol-2-yl)-l H-indole-3 -carbonitrile;
1 -(3 ,5 -Difluoro-4-hydroxy-phenyl)-2-(3 -methyl-thiophen-2-yl)- 1 H-indole-3 -carbonitrile;
1 -(3 ,5-Difluoro-4-hydroxy-phenyl)-2-( 1 -methyl-1 H-pyrazol-4-yl)- 1 H-indole-3 -carbonitrile; l-(3,5-Difluoro-4-hydroxy-phenyl)-2-pyridm-4-yl-lH-indole-3-carbonitrile;
1 -(3 -Chloro-4-hydroxy-phenyl)-2-thiophen-3 -yl- 1 H-indole-3 -carbonitrile; 1 -(3 -Chloro-4-hydroxy-phenyl)-2-thiophen-2-yl- 1 H-indole-3 -carbonitrile;
1 -(3 -Chloro-4-hydroxy-phenyl)-2-(3 , 5 -dimethyl-isoxazol-4-yl)- 1 H-indole-3 -carbonitrile; l-(3-Chloro-4-hydroxy-phenyl)-2-(l-methyl-lH-pyrrol-2-yl)-lH-indole-3-carbonitrile; l-(3-Chloro-4-hydroxy-phenyl)-2-(3-methyl-thiophen-2-yl)-lH-indole-3-carbonitrile; l-(3-Chloro-4-hydroxy-phenyl)-2-pyridin-4-yl-lH-indole-3-carbonitrile; l-(3-Fluoro-4-hydroxy-phenyl)-2-thiophen-3-yl-lH-indole-3-carbonitrile; l-(3-Fluoro-4-hydroxy-phenyl)-2-thiophen-2-yl-lH-indole-3-carbonitrile;
2-(3,5-Dimethyl-isoxazol-4-yl)-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
1 -(3 -Fluoro-4-hydroxy-phenyl)-2-( 1 -methyl-1 H-pyrrol-2-yl)- 1 H-indole-3-carbonitrile;
1 -(3 -Fluoro-4-hydroxy-phenyl)-2-(3 -methyl -thiophen-2-yl)-l H-indole-3 -carbonitrile; 1 -(3 -Fluoro-4-hydroxy-phenyl)-2-( 1 -methyl- 1 H-pyrazol-4-yl)-l H-indole-3 -carbonitrile;
1 -(3 -Fluoro-4-hydroxy-phenyl)-2-pyridin-4-yl- 1 H-indole-3-carbonitrile;
2-Dimethylamino- 1 -(2-fluoro-4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
2-(3,5-Dimethyl-isoxazol-4-yl)-l-(2-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile; l-(3-Fluoro-4-hydroxy-phenyl)-2-((E)-propenyl)-lH-indole-3-carbonitrile; 1 -(3 -Fluoro-4-hydroxy-phenyl)-2-((Z)-propenyl)-l H-indole-3 -carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-((Z)-propenyl)-lH-indole-3-carbonitrile; l-(2,3-Diflυoro-4-hydroxy-phenyl)-2-vinyl-lH-indole-3-carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-thiophen-3-yl-l H-indole-3-carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-thiophen-2-yl-lH-indole-3-carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(3-methyl-thiophen-2-yl)-lH-indole-3-carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(l-methyl-lH-pyrrol-2-yl)-lH-indole-3-carbonitrile;
2-(2-Acetyl-pyrrol-l-yl)-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
1 -(3 -Fluoro-4-hydroxy-phenyl)-2 -pyrrol- 1 -yl- 1 H-indole-3-carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-pyrrol-l -yl-l H-indole-3-carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-prop-l -ynyl-lH-indole-3-carbonitrile;
1 -(3 -Fluoro-4-hydroxy-phenyl)-2-(2-methyl-prop- 1 -enyl)- 1 H-indole-3 -carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(2-methyl-prop-l-enyl)-lH-indole-3-carbonitrile; 2-(2-Acetyl-pyrrol- 1 -yl)- 1 -(2,3 -difluoro-4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
1 -(3-Fluoro-4-hydroxy-phenyl)-2-pyrazol- 1 -yl- 1 H-indole-3 -carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-pyrazol-l-yl-lH-indole-3-carbonitrile;
2-(2,5 -Dimethyl-pyrrol- 1 -yl)- 1 -(3 -fluoro-4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile; 2-(2-Ethyl -pyrrol- 1 -yl)-l -(3 -fluoro-4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
2-(2-Cyano-pyrrol-l-yl)-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
1 -(3 -Fluoro-4-hydroxy-phenyl)-2-(2 -methyl-pyrrol- 1 -yl)- 1 H-indole-3 -carbonitrile; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(2-ethyl-pyrrol-l-yl)-lH-indole-3-carbonitrile;
2-(2-Cyano-pyrrol-l-yl)-l-(2,3-difluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile; 1 -(2,3-Difluoro-4-hydroxy-phenyl)-2-(2-methyl-pyrrol-l -yl)-lH-indole-3-carbonitrile;
1 -(2-Fluoro-4-hydroxy-phenyl)-2-pyrrol- 1 -yl- 1 H-indole-3 -carbonitrile; l-(2,3-difluoro-4-hydroxyphenyl)-2-(3-methylbut-2-enyl)-lH-indole-3-carbonitrile;
[ 1 -(4-Hydroxy-phenyl)-2-phenyl- 1 H-indol-3 -yl] -acetonitrile;
[l-(4-Hydroxy-phenyl)-2-phenyl-lH-indol-3-yl]-acetic acid; 2-[ 1 -(4-Hydroxy-phenyl)-2-phenyl-l H-indol-3 -yl]-acetamide;
4-(3-Isopropenyl-2-phenyl-indol-l-yl)-phenol;
4-[3-(2-Methyl-2H-pyrazol-3-yl)-2-phenyl-indol-l-yl]-phenol;
4-(2-Phenyl-3-thiazol-4-yl-indol-l-yl)-phenol;
4-(2-Phenyl-3 -prop- 1 -ynyl-indol- 1 -yl)-phenol; l-(4-Hydroxy-phenyl)-2-((E)-propenyl)-l H-indole-3 -carboxylic acid, amide;
1 -(4-Hydroxy-phenyl)-2-(2-rnethyl-prop-l -enyl)-l H-indole-3 -carboxylic acid, amide;
1 -(4-Hydroxy-phenyl)-2-((Z)- 1 -methyl -propenyl)- 1 H-indole-3 -carboxylicacid, amide;
4-(2-Phenyl-3-pyrazol-l-yl-indol-l-yl)-phenol;
4-(3-Imidazol-l -yl-2-phenyl-indol-l -yl)-phenol; 4-[3 -(5 -Methyl-pyrazol- 1 -yl)-2-phenyl-indol- 1 -yl] -phenol;
2-Bromo-l-(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid, amide; l-(4-Hydroxy-phenyl)-2-((Z)-3,3,3-trifluoro-propenyl)-lH-indole-3-carbonitrile;
(Z)-2-bromo-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide;
(Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-(lH-pyrrol-l-yl)-lH-indole-3-carboximidamide; 2-(3,5-Dimethyl-isoxazol-4-yl)-l -(4-hydroxy-phenyl)-lH-indole-3-carboxylic acid, amide;
(Z)-N'-hydroxy- 1 -(4-hydroxyphenyl)-2-(2-methylprop- 1 -enyl)- 1 H-indole-3 -carboximidamide;
1 -(4-Hydroxy-phenyl)-2-phenyl-lH-indole-3-carboxylic acid; hydroxyamide;
(Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-phenyl-lH-indole-3-carboximidamide; l-(4-Hydroxy-phenyl)-2-pyrrol-l-yl-lH-indole-3-carboxylic acid, amide; [1 -(4-Hydroxy-phenyl)-2-pyrrol-l -yl-1 H-indol-3 -yl]-carbamic acid tert-butyl ester;
2-(3,5-Dimethyl-isoxazol-4-yl)-l-(4-hydroxy-phenyl)-N-methyl-lH-indole-3-carboxamidine;
Methyl 2-(3,5-dimethylisoxazol-4-yl)-l-(4-hydroxyphenyl)-lH-indole-3-carbimidate; N-((2-(3 ,5-dimethylisoxazol-4-yl)- 1 -(4-hydroxyphenyl)- 1 H-indol-3 -yl)(imino)methyl)acetamide;
2-(5 -ethyl-3 -methylisoxazol-4-yl)- 1 -(4-hydroxyphenyl)- 1 H-indole-3 -carboxamide;
(Z)-2-(2-ethyl-lH-pyrrol-l-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide;
(Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-(2-methyl-lH-pyrrol-l-yl)-lH-indole-3-carboximidamide; l-(4-hydroxyphenyl)-2-(2-methyl-lH-pyrrol-l-yl)-lH-mdole-3-carboxamide;
4-(3 -chloro-2-(3 ,5 -dimethylisoxazol-4-yl)- 1 H-indol- 1 -yl)phenol;
(Z)-2-((Z)-but-2-en-2-yl)-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide;
(Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-(5-methyl-lH-pyrazol-l-yl)-lH-indole-3-carboximidaniide;
(Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-(4-methylthiophen-3-yl)-lH-indole-3-carboximidamide; (Z)-2-(2,5-diniethyl-lH-pyrrol-l -yl)-N'-hydroxy-l -(4-hydroxyphenyl)-lH-indole-3-carboximidamide;
(Z)-N'-hydroxy-l-(4-hydroxyphenyl)-2-phenoxy-lH-indole-3-carboximidamide;
1 -(4-Hydroxy-phenyl)-2-phenyl- 1 H-indole-3 -carboxylic acid;
2-(3,5-Dimethyl-isoxazol-4-yl)-l-(3-fluoro-4-hydroxy-phenyl)-lH-indole-3-carboxylic acid;
2-(3,5-Dimethyl-isoxazol-4-yl)-l-(2-fluoro-4-hydroxy-phenyl)-lH-indole-3-carboxylic acid; l-(2,3-Difluoro-4-hydroxy-phenyl)-2-(3,5-dimethyl-isoxazol-4-yl)-lH-indole-3-carboxylic acid; l-(4-Hydroxy-phenyl)-2-((Z)-propenyl)-lH-indole-3-carboxylic acid;
1 -(4-Hydroxy-phenyl)-2-((E)-propenyl)- 1 H-indole-3 -carboxylic acid; l-(4-Hydroxy-phenyl)-2-(2-methyl-prop-l-enyl)-lH-indole-3-carboxylic acid;
1 -(4-Hydroxy-phenyl)-2-(2-methyl-allyl)- 1 H-indole-3 -carboxylic acid; 1 -(4-Hydroxy-phenyl)-2-((Z)-l -methyl-propenyl)-lH-indole-3-carboxylicacid; l-(3-Fluoro-4-hydroxy-phenyl)-2-thiophen-3-yl-lH-indole-3-carboxylic acid;
1 -(3 -Fluoro-4-hydroxy-phenyl)-2-thiophen-2-yl- 1 H-indole-3 -carboxylic acid;
1 -(3-Fluoro-4-hydroxy-phenyl)-2-(l -methyl-1 H-pyrrol-2-yl)-l H-indole-3 -carboxylic acid; l-(3-Fluoro-4-hydroxy-phenyl)-2-(3-methyl-thiophen-2-yl)-lH-indole-3-carboxylic acid; 2-Bromo-l-(4-hydroxy-phenyl)-l H-indole-3 -carboxylic acid; l-(4-Hydroxy-phenyl)-2-pyrrol-l-yl-lH-indole-3-carboxylic acid;
2,7-Dibromo-l-(2,5-difluoro-4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
2-Bromo-4-fluoro-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
4-Fluoro-l-(4-hydroxy-phenyl)-2-pyrrol-l-yl-lH-indole-3-carbonitrile; 4-Fluoro- 1 -(4-hydroxy-phenyl)-2-phenyl- 1 H-indole-3 -carbonitrile;
2-(3,5-Dimethyl-isoxazol-4-yl)-4-fluoro-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
4-Fluoro-l-(4-hydroxy-phenyl)-2-(2-methyl-prop-l-enyl)-lH-indole-3-carbonitrile;
5-Fluoro-l-(4-hydroxy-phenyl)-2-phenyl-lH-indole-3-carbonitrile;
2-(3 ,5 -Dimethyl-isoxazol-4-yl)-5 -fluoro- 1 -(4-hydroxy -phenyl)- 1 H-indole-3 -carbonitrile; 5-Fluoro-l -(4-hydroxy-phenyl)-2-pyrrol-l -yl-1 H-indole-3 -carbonitrile;
5-Fluoro-l-(4-hydroxy-phenyl)-2-(2-methyl-prop-l-enyl)-lH-indole-3-carbonitrile; (Z)-2-(3,5-dimethylisoxazol-4-yl)-5-fluoro-N'-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3- carboximidamide;
(Z)-2-(3,5-dimethylisoxazol-4-yl)-4-fluoro-Nl-hydroxy-l-(4-hydroxyphenyl)-lH-indole-3- carboximidamide; (Z)-5 -fluoro-N' -hydroxy- 1 -(4-hydroxyphenyl)-2-(2-methylprop- 1 -enyl)-l H-indole-3 -carboximidamide;
4-Chloro-2-(3 ,5 -dimethyl-isoxazol-4-yl)-l -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
2-(3,5-dimethylisoxazol-4-yl)-4,5-difluoro-l-(4-hydroxyphenyl)-lH-indole-3-carbonitrile;
2-(4-cyano- 1 -methyl- 1 H-pyrazol-5 -yl)-4-fluoro- 1 -(4-hydroxyphenyl)- 1 H-indole-3 -carbonitrile;
2-(3,5-dimethylisoxazol-4-yl)-5-fluoro-l-(4-hydroxyphenyl)-lH-indole-3-carboximidamide; 2-(3,5-dimethylisoxazol-4-yl)-5-fluoro-l -(4-hydroxyphenyl)-lH-indole-3-carboxamide;
2-Bromo-7 -fluoro- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
2-(3,5-Dimethyl-isoxazol-4-yl)-7-fluoro-l-(4-hydroxy-phenyl)-lH-indole-3-carbonitrile;
2-(3 ,5 -Dimethyl-isoxazol-4-yl)-4,7-difluoro- 1 -(4-hydroxy-phenyl)- 1 H-indole-3 -carbonitrile;
(Z)-2-(3 ,5 -dimethylisoxazol-4-yl)-4,7-difluoro-N'-hydroxy- 1 -(4-hydroxyphenyl)- 1 H-indole-3 - carboximidamide;
1 -(2,5 -Difluoro-4-hydroxy-phenyl)-2-(3 ,5 -dimethyl -isoxazol-4-yl)-l H-indole-3 -carbonitrile;
1 -(3-bromo-4-hydroxyphenyl)-2-(2-methylprop- 1 -enyl)- 1 H-indole-3 -carboxamide;
(Z)-2-(3,5-dimethylisoxazol-4-yl)-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-lH-indole-3- carboximidamide; (Z)-I -(2,5-difluoro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-N'-hydroxy-l H-indole-3- carboximidamide;
(Z)-l-(3,5-difluoro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-N'-hydroxy-lH-indole-3- carboximidamide;
(Z)-2-(3,5-dimethylisoxazol-4-yl)-l-(3-fluoro-4-hydroxyphenyl)-N'-hydroxy-lH-indole-3- carboximidamide;
(Z)-I -(3-chloro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-N'-hydroxy-l H-indole-3- carboximidamide;
2-(3 ,5 -dimethylisoxazol-4-yl)- 1 -(2-fluoro-4-hydroxyphenyl)- 1 H-indole-3 -carboxamide;
(Z)-l-(2,3-difluoro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-N'-hydroxy-lH-indole-3- carboximidamide; l-(2,3-difluoro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-lH-indole-3-carboxamide; l-(2-fluoro-4-hydroxyphenyl)-2-(3-methylthiophen-2-yl)-lH-indole-3-carbonitrile;
2-(3,5-dimethyl-lH-pyrazol-4-yl)-l-(2-fluoro-4-hydroxyphenyl)-lH-indole-3-carbonitrile;
1 -(2-fluoro-4-hydroxyphenyl)-2-( 1 -methyl- 1 H-pyrazol-5 -yl)- 1 H-indole-3 -carbonitrile; l-(2-fluoro-4-hydroxyphenyl)-2-(l,3I5-trimethyl-lH-pyrazol-4-yl)-lH-indole-3-carbonitrile; l-(2-fluoro-4-hydroxyphenyl)-2-(3-(trifluoromethyl)-lH-pyrazol-4-yl)-lH-indole-3-carbonitrile;
(Z)-I -(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(3-methylthiophen-2-yl)-lH-indole-3-carboximidamide; (Z)-I -(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-( 1 -methyl- 1 H-pyrazol-5 -yl)-l H-indole-3 - carboximidamide;
(Z)-I -(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(l,3,5-trimethyl-lH-pyrazol-4-yl)-lH-indole-3- carboximidamide; (Z)-4-fluoro-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(l-methyl-lH-pyrazol-5-yl)-lH-indole-3- carboximidamide;
(Z)-4-fluoro-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(l,3,5-trimethyl-lH-pyrazol-4-yl)-lH-indole-3- carboximidamide;
(Z)-2-(3,5-dimethyl-lH-pyrazol-4-yl)-4-fluoro-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-lH-indole-3- carboximidamide;
(Z)-4-fluoro-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(3-methylthiophen-2-yl)-lH-indole-3- carboximidamide;
(Z)-2-(3 ,5 -dimethylisoxazol-4-yl)-4-fluoro- 1 -(2-fluoro-4-hydroxyphenyl)-N'-hydroxy- 1 H-indole-3 - carboximidamide; (Z)-I -(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(3-methylthiophen-2-yl)-lH-indole-3-carboximidamide;
(Z)-I -(2-fluoro-4-hydroxyphenyl)-N' -hydroxy-2 -(I -methyl-lH-pyrazol-5-yl)-lH-indole-3- carboximidamide;
(Z)-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(l,3,5-trimethyl-lH-pyrazol-4-yl)-lH-indole-3- carboximidamide; (Z)-2-(3,5-dimethyl-lH-pyrazol-4-yl)-l-(2-fluoro-4-hydroxyphenyl)-N'-hydroxy-lH-indole-3- carboximidamide; methyl 2-(3 ,5 -dimethylisoxazol-4-yl)- 1 -(2-fluoro-4-hydroxyphenyl)- 1 H-indole-3 -carbimidate;
2-(3 ,5 -dimethylisoxazol-4-yl)- 1 -(3 -fluoro-4-hydroxyphenyl)- 1 H-indole-3 -carboxamide; l-(2,5-difluoro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-lH-indole-3-carboximidamide; 2-(3 ,5 -dimethylisoxazol-4-yl)- 1 -(3 -fluoro-4-hydroxyphenyl)- 1 H-indole-3 -carboximidamide;
(Z)-I -(3 -fluoro-4-hydroxyphenyl)-N'-hydroxy-2-( 1 H-pyrrol- 1 -yl)- 1 H-indole-3 -carboximidamide; l-(3-fluoro-4-hydroxyphenyl)-2-(lH-pyrrol-l-yl)-lH-indole-3-carboxamide;
(Z)-l-(2,3-difluoro-4-hydroxyphenyl)-N'-hydroxy-2-(lH-pyrrol-l-yl)-lH-mdole-3-carboximidamide; l-(2,3-difluoro-4-hydroxyphenyl)-2-(lH-pyrrol-l-yl)-lH-indole-3-carboxamide; (Z)-2-(2,5-dimethyl-lH-pyrrol-l-yl)-l-(3-fluoro-4-hydroxyphenyl)-N'-hydroxy-lH-indole-3- carboximidamide;
(Z)-I -(3 -fluoro-4-hydroxyphenyl)-N'-hydroxy-2-(2-methyl- 1 H-pyrrol- 1 -yl)- 1 H-indole-3 - carboximidamide; l-(3,5-difluoro-4-hydroxyphenyl)-2-(3,5-dimethylisoxazol-4-yl)-lH-indole-3-carboxamide; and (Z)-2-(3,5-dimethylisoxazol-4-yl)-6-fluoro-N'-hydroxy-l-(4-hydroxyphenyl)-lΗ-indole-3- carboximidamide; or a pharmaceutically acceptable ester, amide, carbamate, solvate or salt thereof, including a salt of such an ester, amide or carbamate, and a solvate of such an ester, amide, carbamate or salt thereof.
19. A pharmaceutical composition which comprises a compound as claimed in any one of claims 1 to 18, together with a pharmaceutically acceptable carrier.
20. A compound as claimed in any one of claims 1 to 18, for use as a medicament.
21. A compound as claimed in claim 20, for use in the treatment or prophylaxis of a condition associated with a disease or disorder associated with estrogen receptor activity.
22. Use of a compound as claimed in any one of claims 1 to 18, for the manufacture of a medicament for the treatment or prophylaxis of a condition associated with a disease or disorder associated with estrogen receptor activity.
23. A method for the treatment or prophylaxis of a disease or disorder associated with estrogen receptor activity in a mammal, which comprises administering to the mammal a therapeutically effective amount of a compound as claimed in any one of claims 1 to 18 or a composition as claimed in claim 19.
24. Use of a compound as claimed in any one of claims 1 to 18 in labelled form as a diagnostic agent for the diagnosis of conditions associated with a disease or disorder associated with estrogen receptor activity, or use of a compound as claimed in any one of claims 1 to 18 or a labelled form of such a compound as a reference compound in a method of identifying ligands for the estrogen receptor.
25. A compound as claimed in claim 21 , a method as claimed in claim 23, or a use as claimed in either claim 22 or claim 24, wherein the condition associated with a disease or disorder associated with estrogen receptor activity is selected from bone loss, bone fractures, osteoporosis, cartilage degeneration, endometriosis, uterine fibroid disease, hot flashes, increased levels of LDL cholesterol, cardiovascular disease, impairment of cognitive functioning, age-related mild cognitive impairment, cerebral degenerative disorders, restenosis, gynecomastia, vascular smooth muscle cell proliferation, obesity, incontinence, anxiety, depression, perimenopausal depression, post-partum depression, premenstrual syndrome, manic depression, dementia, obsessive compulsive behavior, attention deficit disorder, attention deficit hyperactivity disorder, sleep disorders, irritability, impulsivity, anger management, hearing disorders, multiple sclerosis, Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, spinal cord injury, stroke, autoimmune disease, inflammation, IBD, IBS, sexual dysfunction, hypertension, retinal degeneration, lung cancer, colon cancer, breast cancer, uterus cancer, prostate cancer and cholangiocarcinoma.
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