WO2009092864A3 - Spectrofluorometer using monochromators - Google Patents

Spectrofluorometer using monochromators Download PDF

Info

Publication number
WO2009092864A3
WO2009092864A3 PCT/FI2009/050058 FI2009050058W WO2009092864A3 WO 2009092864 A3 WO2009092864 A3 WO 2009092864A3 FI 2009050058 W FI2009050058 W FI 2009050058W WO 2009092864 A3 WO2009092864 A3 WO 2009092864A3
Authority
WO
WIPO (PCT)
Prior art keywords
light beam
monochromator
sample
measurement
spectrally
Prior art date
Application number
PCT/FI2009/050058
Other languages
French (fr)
Other versions
WO2009092864A2 (en
Inventor
Raimo Harju
Petri Kivelä
Jyrki Laitinen
Pauli Salmelainen
Jarkko Sarmaala
Original Assignee
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wallac Oy filed Critical Wallac Oy
Priority to EP09704552A priority Critical patent/EP2235506A2/en
Priority to US12/739,146 priority patent/US20100308234A1/en
Publication of WO2009092864A2 publication Critical patent/WO2009092864A2/en
Publication of WO2009092864A3 publication Critical patent/WO2009092864A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Spectrometry And Color Measurement (AREA)

Abstract

The invention concerns a measurement system and method for optical spectroscopic measurement of samples, in particular fluorescence spectroscopic measurements. The system comprises an illumination source (101, 102, 103) for forming a primary light beam, a first tunable monochromator (106) for spectrally filtering the primary light beam, a sample-receiving zone (109, 114) to which the spectrally filtered primary beam is directed for producing a secondary light beam affected by a sample in the sample receiving zone, and a second tunable monochromator (124) for spectrally filtering the secondary light beam, and a detector (130) for measuring the intensity of the spectrally filtered secondary beam. In particular, the system is adapted to scan a predefined wavelength range using one of the monochromators and totune the other monochromator sequentially to one of at least two predefined separate wavelengths in order to eliminate the effect of undesired diffraction orders of the second monochromator on the measurement. The invention allows for eliminating the use of optical diffraction order filters on the emission side of a fluorescence measurement system.
PCT/FI2009/050058 2008-01-25 2009-01-23 Spectrofluorometer using monochromators WO2009092864A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09704552A EP2235506A2 (en) 2008-01-25 2009-01-23 Spectrofluorometer using monochromators
US12/739,146 US20100308234A1 (en) 2008-01-25 2009-01-23 Improved Measurement System and Method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US666008P 2008-01-25 2008-01-25
FI20085062 2008-01-25
FI20085062A FI20085062A0 (en) 2008-01-25 2008-01-25 Improved measurement system and method
US61/006,660 2008-01-25

Publications (2)

Publication Number Publication Date
WO2009092864A2 WO2009092864A2 (en) 2009-07-30
WO2009092864A3 true WO2009092864A3 (en) 2009-09-24

Family

ID=39004362

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI2009/050058 WO2009092864A2 (en) 2008-01-25 2009-01-23 Spectrofluorometer using monochromators

Country Status (4)

Country Link
US (1) US20100308234A1 (en)
EP (1) EP2235506A2 (en)
FI (1) FI20085062A0 (en)
WO (1) WO2009092864A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8901513B2 (en) * 2011-03-08 2014-12-02 Horiba Instruments, Incorporated System and method for fluorescence and absorbance analysis
US10184892B2 (en) 2014-10-29 2019-01-22 Horiba Instruments Incorporated Determination of water treatment parameters based on absorbance and fluorescence
US9670072B2 (en) 2014-10-29 2017-06-06 Horiba Instruments Incorporated Determination of water treatment parameters based on absorbance and fluorescence
EP3620838A1 (en) * 2015-06-02 2020-03-11 Life Technologies Corporation Systems and methods for calibrating a structured illumination imaging system and for capturing a structured illumination image
EP3299780A1 (en) * 2016-09-26 2018-03-28 Berthold Technologies GmbH & Co. KG Method and system for spectroscopic measurement of optical properties of samples

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2024412A (en) * 1978-06-05 1980-01-09 Hitachi Ltd Fluorescence spectrophotometer
US4575243A (en) * 1982-07-01 1986-03-11 Bodenseewerk Perkin-Elmer & Co., Gmbh Monochromator
US4626684A (en) * 1983-07-13 1986-12-02 Landa Isaac J Rapid and automatic fluorescence immunoassay analyzer for multiple micro-samples
US5285254A (en) * 1991-03-25 1994-02-08 Richard J De Sa Rapid-scanning monochromator with moving intermediate slit
US20050030607A1 (en) * 2003-07-14 2005-02-10 Lutz Niggl Arrangement and use of a slit diaphragm
US20070065074A1 (en) * 2001-09-27 2007-03-22 Bio-Rad Laboratories, Inc. A Corporation Of The State Of Delaware Biochemical assay detection using a fiber optic exciter

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4037961A (en) * 1976-07-06 1977-07-26 Baxter Travenol Laboratories, Inc. System and apparatus for contour plotting the total luminescence spectrum of a sample
US4549807A (en) * 1983-10-07 1985-10-29 At&T Bell Laboratories Process for measuring fluorescence
US5475221A (en) * 1994-05-11 1995-12-12 Brimrose Corporation Of America Optical spectrometer using light emitting diode array
DE19748211A1 (en) * 1997-10-31 1999-05-06 Zeiss Carl Fa Optical array system and reader for microtiter plates

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2024412A (en) * 1978-06-05 1980-01-09 Hitachi Ltd Fluorescence spectrophotometer
US4575243A (en) * 1982-07-01 1986-03-11 Bodenseewerk Perkin-Elmer & Co., Gmbh Monochromator
US4626684A (en) * 1983-07-13 1986-12-02 Landa Isaac J Rapid and automatic fluorescence immunoassay analyzer for multiple micro-samples
US5285254A (en) * 1991-03-25 1994-02-08 Richard J De Sa Rapid-scanning monochromator with moving intermediate slit
US20070065074A1 (en) * 2001-09-27 2007-03-22 Bio-Rad Laboratories, Inc. A Corporation Of The State Of Delaware Biochemical assay detection using a fiber optic exciter
US20050030607A1 (en) * 2003-07-14 2005-02-10 Lutz Niggl Arrangement and use of a slit diaphragm

Also Published As

Publication number Publication date
EP2235506A2 (en) 2010-10-06
WO2009092864A2 (en) 2009-07-30
US20100308234A1 (en) 2010-12-09
FI20085062A0 (en) 2008-01-25

Similar Documents

Publication Publication Date Title
JP6075788B2 (en) Fluorescence and absorption analysis systems and methods
Bégin et al. Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system
ATE449317T1 (en) AUTONOMOUS CALIBRATION FOR OPTICAL ANALYSIS SYSTEMS
US20090152454A1 (en) Method and apparatus for automated spectral calibration
US20080174768A1 (en) Self referencing LED detection system for spectroscopy applications
CA2664133A1 (en) A method for correcting a spectral image for optical aberrations using software
DK2895844T3 (en) Apparatus with an arrangement of optical elements
WO2009092864A3 (en) Spectrofluorometer using monochromators
JP6888085B2 (en) Raman spectroscopy and equipment
WO2007047017A3 (en) Optical spectroscopy utilizing planar spectral filters
Biswas et al. Recent developments in smartphone spectrometer sample analysis
WO2016082804A1 (en) Device and method for obtaining raman scattering spectrum
US20110085164A1 (en) Method and apparatus for automated spectral calibration
Adami et al. Light-emitting diode based shifted-excitation Raman difference spectroscopy (LED-SERDS)
US20220236109A1 (en) System and method for hyperspectral imaging in highly scattering media by the spectral phasor approach using two filters
EP1975602B1 (en) Optical analyzer
Zwinkels et al. Spectral fluorescence measurements
KR101632672B1 (en) Confocal spectrogram microscope
Conmy et al. Calibration and performance of a new in situ multi-channel fluorometer for measurement of colored dissolved organic matter in the ocean
US20180195965A1 (en) Method of measuring raman scattering and related spectrometers and laser sources
Wang et al. Low resolution Raman: The impact of spectral resolution on limit of detection and imaging speed in hyperspectral imaging
JP2004219330A (en) Fluorescence measuring apparatus
RU2478192C2 (en) Method for optical remote detection of compounds in medium
MX2010003883A (en) Spectrometry device for fluid analysis.
Prakash et al. White light excitation fluorescence (WLEF) Part I. Exploring the use in analytical fluorimetry

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09704552

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 12739146

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2009704552

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE