WO2009088216A1 - L-threonine producing escherichia coli and process for producing l-threonine using same - Google Patents
L-threonine producing escherichia coli and process for producing l-threonine using same Download PDFInfo
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- WO2009088216A1 WO2009088216A1 PCT/KR2009/000066 KR2009000066W WO2009088216A1 WO 2009088216 A1 WO2009088216 A1 WO 2009088216A1 KR 2009000066 W KR2009000066 W KR 2009000066W WO 2009088216 A1 WO2009088216 A1 WO 2009088216A1
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- threonine
- ppc
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- escherichia coli
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
- C12N15/04—Fungi
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Definitions
- ⁇ ⁇ ⁇ ppc ⁇ ⁇ ⁇ ⁇ DNA ⁇ ⁇ ⁇ , ⁇ pJW168 ⁇ (BMG Biothechnol. 2001;1:7. Epub 2001 Sep. 26) ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ppc ⁇ ⁇ ⁇ cysK ⁇ ⁇ ⁇ ⁇ KCCM 10541-PcyK-ppc ⁇ ⁇ .
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- General Chemical & Material Sciences (AREA)
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- Biophysics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
Description
조성물 | 농도 ( 리터당) |
포도당 | 70 g |
KH2PO4 | 2 g |
(NH4)2SO4 | 25 g |
MgSO4 · 7H2O | 1 g |
FeSO4 · 7H2O | 5 mg |
MnSO4 · 4H2O | 5 mg |
DL- 메티오닌 | 0.15 g |
효모 추출물 | 2 g |
탄산칼슘 | 30 g |
pH | 6.8 |
균주 | L- 쓰레오닌 (g/L) |
KCCM10541 ( 모균주) | 29.8 |
KCCM10541-PcysK-ppc | 34.7 |
Claims (4)
- 염색체상의 포스포에놀 파이루베이트 카르복실라제 유전자(ppc)의 프로모터가 시스테인 신타아제 유전자(cysK)의 프로모터로 교체된, L- 쓰레오닌 생산능을 갖는 대장균(Escherichia coli ).
- 제1항에 있어서, 상기 대장균은 메티오닌 요구성, 쓰레오닌 유사체에 대한 내성, 라이신 유사체에 대한 내성, 이소루이신 유사체에 대한 내성 및 메티오닌 유사체에 대한 내성을 갖는 것을 특징으로 하는 것인 대장균.
- 제1항에 있어서, 상기 대장균은 대장균 CA030031( 수탁번호 KCCM 10910) 인 것인 대장균.
- 제1항 내지 제3항 중 어느 한 항에 따른 대장균을 배양하는 단계 및 상기 대장균의 배양액으로부터 L- 쓰레오닌을 분리하는 단계를 포함하는 것을 특징으로 하는 L- 쓰레오닌을 생산하는 방법.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/812,203 US8455237B2 (en) | 2008-01-08 | 2009-01-07 | Escherichia coli strain with phosphoenolpyruvate carboxylase promoter replaced with cysteine synthase promoter and method of enhanced production of L-threonine using the same |
BRPI0925344A BRPI0925344B1 (en) | 2008-01-08 | 2009-01-07 | l-threonine producing escherichia coli strain and l-threonine production method |
ES09700769.4T ES2535732T3 (en) | 2008-01-08 | 2009-01-07 | Escherichia coli producing L-threonine and process to produce L-threonine using the same |
PL09700769T PL2233570T3 (en) | 2008-01-08 | 2009-01-07 | L-threonine producing escherichia coli and process for producing l-threonine using same |
CN2009801055973A CN101946002B (en) | 2008-01-08 | 2009-01-07 | L-threonine producing escherichia coli and process for producing l-threonine using same |
EP09700769.4A EP2233570B1 (en) | 2008-01-08 | 2009-01-07 | L-threonine producing escherichia coli and process for producing l-threonine using same |
US13/658,369 US8486670B2 (en) | 2008-01-08 | 2012-10-23 | Method of producing L-threonine using Escherichia coli strain with phosphoenolpyruvate carboxylase promoter replaced with cysteine synthase promoter |
Applications Claiming Priority (2)
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KR10-2008-0002310 | 2008-01-08 | ||
KR1020080002310A KR100966324B1 (en) | 2008-01-08 | 2008-01-08 | Escherichia coli strain with enhanced L-threonine productivity and method of producing L-threonine using the same |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/812,203 A-371-Of-International US8455237B2 (en) | 2008-01-08 | 2009-01-07 | Escherichia coli strain with phosphoenolpyruvate carboxylase promoter replaced with cysteine synthase promoter and method of enhanced production of L-threonine using the same |
US13/658,369 Division US8486670B2 (en) | 2008-01-08 | 2012-10-23 | Method of producing L-threonine using Escherichia coli strain with phosphoenolpyruvate carboxylase promoter replaced with cysteine synthase promoter |
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WO2009088216A1 true WO2009088216A1 (en) | 2009-07-16 |
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PCT/KR2009/000066 WO2009088216A1 (en) | 2008-01-08 | 2009-01-07 | L-threonine producing escherichia coli and process for producing l-threonine using same |
Country Status (8)
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US (2) | US8455237B2 (en) |
EP (1) | EP2233570B1 (en) |
KR (1) | KR100966324B1 (en) |
CN (1) | CN101946002B (en) |
BR (1) | BRPI0925344B1 (en) |
ES (1) | ES2535732T3 (en) |
PL (1) | PL2233570T3 (en) |
WO (1) | WO2009088216A1 (en) |
Families Citing this family (13)
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CN102304553B (en) * | 2011-09-20 | 2013-09-04 | 中国科学院微生物研究所 | Method for producing L-threonine |
KR101327093B1 (en) | 2012-01-06 | 2013-11-07 | 씨제이제일제당 (주) | Microorganisms producing l-amino acids and process for producing l-amino acids using thereof |
KR101555749B1 (en) | 2013-10-23 | 2015-09-25 | 씨제이제일제당 (주) | Microorganisms for production of o-succinyl homoserine and method for production of o-succinyl homoserine using the same |
KR101555750B1 (en) | 2013-10-23 | 2015-09-25 | 씨제이제일제당 (주) | Microorganisms for production of o-succinyl homoserine and method for production of o-succinyl homoserine using the same |
KR101599800B1 (en) | 2014-03-21 | 2016-03-04 | 씨제이제일제당 주식회사 | Microorganisms having Enhanced L-Amino acids Productivity and Process for Producing L-Amino acids Using the Same |
WO2016036209A1 (en) * | 2014-09-05 | 2016-03-10 | 씨제이제일제당 주식회사 | Microorganism with improved l-threonine productivity, and method for producing l-threonine by using same |
KR101804017B1 (en) * | 2015-10-23 | 2017-12-04 | 씨제이제일제당 (주) | Microorganisms having L-threonine productivity and a process for producing L-threonine using the same |
KR101996767B1 (en) | 2018-11-29 | 2019-07-04 | 씨제이제일제당 (주) | cAMP receptor protein variant and method for producing L-amino acid using the same |
KR101991206B1 (en) | 2018-11-29 | 2019-06-19 | 씨제이제일제당 (주) | cAMP receptor protein variant and method for producing L-amino acid using the same |
KR101991207B1 (en) | 2018-11-29 | 2019-06-19 | 씨제이제일제당 (주) | cAMP receptor protein variant and method for producing L-amino acid using the same |
PE20220018A1 (en) * | 2019-06-14 | 2022-01-11 | Cj Cheiljedang Corp | COMPOSITION TO PREVENT, TREAT OR IMPROVE GASTROINTESTINAL DISEASES THAT COMPRISES A STRAIN OF THE GENUS CORYNEBACTERIUM AND CULTIVATION OF THE SAME |
CN114317389B (en) * | 2021-12-22 | 2023-06-13 | 南京工业大学 | Method for producing L-threonine by co-culture fermentation of recombinant escherichia coli |
CN115197887B (en) * | 2022-03-15 | 2023-08-25 | 江南大学 | Full-biological synthesis method for producing pimelic acid by utilizing Kleisen condensation reaction |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5610037B1 (en) | 1969-08-04 | 1981-03-05 | ||
KR920008395Y1 (en) | 1990-10-13 | 1992-11-21 | 주식회사서흥캅셀 | Capsules |
JPH05227977A (en) | 1991-09-20 | 1993-09-07 | Ajinomoto Co Inc | Creation of new l-threonine-producing bacterium from esherrichia coli and production of l-threonine by the same bacterium |
US5538873A (en) | 1988-10-25 | 1996-07-23 | Ajinomoto Co., Inc. | Bacterial strain of escherichia coli BKIIM B-3996 as the producer of l-threonine |
US5939307A (en) | 1996-07-30 | 1999-08-17 | The Archer-Daniels-Midland Company | Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production |
WO2002031172A2 (en) * | 2000-10-12 | 2002-04-18 | Basf Aktiengesellschaft | L-threonine producing e. coli strain |
KR100478468B1 (en) * | 2003-09-06 | 2005-03-23 | 씨제이 주식회사 | A method for producing L-threonine |
US20060205044A1 (en) * | 2005-03-11 | 2006-09-14 | D Elia John N | Escherichia coli strains that over-produce L-threonine and processes for their production |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR920009598B1 (en) | 1990-10-11 | 1992-10-21 | 주식회사삼성전자 | Clamping device |
KR920008365B1 (en) * | 1990-12-31 | 1992-09-26 | 제일제당 주식회사 | Method for producing l-threonine |
EP1686184B1 (en) | 2001-07-11 | 2008-08-27 | Evonik Degussa GmbH | Process for the preparation of L-threonine using strains of the enterobacteriaceae family |
DE10314618A1 (en) * | 2003-04-01 | 2004-10-14 | Degussa Ag | Process for the preparation of L-amino acids using strains of the family Enterobacteriaceae |
RU2275425C2 (en) * | 2003-11-03 | 2006-04-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Bacterium belonging to genus escherichia as producer of l-cysteine and method for preparing l-cysteine |
KR100576342B1 (en) * | 2004-02-05 | 2006-05-03 | 씨제이 주식회사 | A microorganism producing L-threonine having an inactivated galR gene, method for producing the same and method for producing L-threonine using the microorganism |
KR100596372B1 (en) * | 2004-12-06 | 2006-07-04 | 씨제이 주식회사 | - - A microorganism producing L-threonine having an inactivated lysR gene method for producing the same and method for producing L-threonine using the microorganism |
KR100620092B1 (en) * | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | Novel promoter nucleic acid derived from corynebacterium genus bacteria expression cassette comprising the promoter and vector comprising the cassette host cell comprising the vector and method for expressing a gene using the cell |
KR100768750B1 (en) | 2006-10-18 | 2007-10-19 | 씨제이 주식회사 | 5'- 5'- A microorganism whose biotin carboxylase activity is enhanced for producing 5'-inosinic acid and method for producing 5'-inonsinic acid using the same microorganism |
ES2534375T3 (en) | 2007-04-11 | 2015-04-22 | Cj Cheiljedang Corporation | Methionine production compositions and procedures |
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- 2009-01-07 PL PL09700769T patent/PL2233570T3/en unknown
- 2009-01-07 US US12/812,203 patent/US8455237B2/en active Active
- 2009-01-07 ES ES09700769.4T patent/ES2535732T3/en active Active
- 2009-01-07 WO PCT/KR2009/000066 patent/WO2009088216A1/en active Application Filing
- 2009-01-07 EP EP09700769.4A patent/EP2233570B1/en active Active
- 2009-01-07 BR BRPI0925344A patent/BRPI0925344B1/en active IP Right Grant
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Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5610037B1 (en) | 1969-08-04 | 1981-03-05 | ||
US5538873A (en) | 1988-10-25 | 1996-07-23 | Ajinomoto Co., Inc. | Bacterial strain of escherichia coli BKIIM B-3996 as the producer of l-threonine |
KR920008395Y1 (en) | 1990-10-13 | 1992-11-21 | 주식회사서흥캅셀 | Capsules |
JPH05227977A (en) | 1991-09-20 | 1993-09-07 | Ajinomoto Co Inc | Creation of new l-threonine-producing bacterium from esherrichia coli and production of l-threonine by the same bacterium |
US5939307A (en) | 1996-07-30 | 1999-08-17 | The Archer-Daniels-Midland Company | Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production |
WO2002031172A2 (en) * | 2000-10-12 | 2002-04-18 | Basf Aktiengesellschaft | L-threonine producing e. coli strain |
KR100478468B1 (en) * | 2003-09-06 | 2005-03-23 | 씨제이 주식회사 | A method for producing L-threonine |
US20060205044A1 (en) * | 2005-03-11 | 2006-09-14 | D Elia John N | Escherichia coli strains that over-produce L-threonine and processes for their production |
Non-Patent Citations (7)
Title |
---|
BMG BIOTHECHNOL., vol. 1, no. 7, 2001 |
NAGY A., GENESIS, vol. 26, 2000, pages 99 |
PROC. NATL. ACAD. SCI. U.S.A., vol. 97, no. 12, 2000, pages 6640 - 6645 |
See also references of EP2233570A4 * |
SUZUKI, APPL. ENVIRON. MICROBIOL., vol. 71, 2005, pages 8472 |
TURBA E ET AL., AGRIC. BIOL. CHEM., vol. 53, 1989, pages 2269 - 2271 |
WARNER ET AL., PNAS, 2000, pages 6640 |
Also Published As
Publication number | Publication date |
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EP2233570A4 (en) | 2011-03-02 |
PL2233570T3 (en) | 2015-11-30 |
US20130040347A1 (en) | 2013-02-14 |
US8486670B2 (en) | 2013-07-16 |
BRPI0925344B1 (en) | 2019-01-22 |
US20110189739A1 (en) | 2011-08-04 |
ES2535732T3 (en) | 2015-05-14 |
CN101946002B (en) | 2013-05-08 |
KR20090076389A (en) | 2009-07-13 |
CN101946002A (en) | 2011-01-12 |
EP2233570B1 (en) | 2015-03-18 |
EP2233570A1 (en) | 2010-09-29 |
KR100966324B1 (en) | 2010-06-28 |
US8455237B2 (en) | 2013-06-04 |
BRPI0925344A2 (en) | 2016-07-05 |
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