WO2009087681A3 - Methods for characterisation of mammalian embryonic stem cells by multiplex pcr - Google Patents
Methods for characterisation of mammalian embryonic stem cells by multiplex pcr Download PDFInfo
- Publication number
- WO2009087681A3 WO2009087681A3 PCT/IN2008/000868 IN2008000868W WO2009087681A3 WO 2009087681 A3 WO2009087681 A3 WO 2009087681A3 IN 2008000868 W IN2008000868 W IN 2008000868W WO 2009087681 A3 WO2009087681 A3 WO 2009087681A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- stem cells
- embryonic stem
- characterisation
- methods
- multiplex pcr
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Abstract
The present disclosure relates to a rapid, cost effective, robust and sensitive method for routine testing of embryonic stem cells. The present disclosure in particular provides a simple inexpensive and definitive multitasked semi-quantitative multiplex RT-PCR system for human ES cell characterization.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN2594MU2007 | 2007-12-28 | ||
IN2594/MUM/2007 | 2007-12-28 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009087681A2 WO2009087681A2 (en) | 2009-07-16 |
WO2009087681A3 true WO2009087681A3 (en) | 2009-09-11 |
Family
ID=40786835
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IN2008/000868 WO2009087681A2 (en) | 2007-12-28 | 2008-12-26 | Methods for characterisation of mammalian embryonic stem cells by multiplex pcr |
Country Status (1)
Country | Link |
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WO (1) | WO2009087681A2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111575387A (en) * | 2020-05-29 | 2020-08-25 | 云南中科胚胎工程生物技术有限公司 | Method for identifying sex of early embryo of Dorper sheep |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060286544A1 (en) * | 2005-05-17 | 2006-12-21 | Arundhati Mandal | Establishment of a human embryonic stem cell line using mammalian cells |
US20070048757A1 (en) * | 2005-05-31 | 2007-03-01 | Applera Corporation | Methods for characterizing cells using amplified micro rnas |
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2008
- 2008-12-26 WO PCT/IN2008/000868 patent/WO2009087681A2/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060286544A1 (en) * | 2005-05-17 | 2006-12-21 | Arundhati Mandal | Establishment of a human embryonic stem cell line using mammalian cells |
US20070048757A1 (en) * | 2005-05-31 | 2007-03-01 | Applera Corporation | Methods for characterizing cells using amplified micro rnas |
Non-Patent Citations (6)
Title |
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CLAYTON HELEN ET AL: "Growth and differentiation of progenitor/stem cells derived from the human mammary gland", EXPERIMENTAL CELL RESEARCH, ACADEMIC PRESS, US, vol. 297, no. 2, 30 April 2004 (2004-04-30), pages 444 - 460, XP002372634, ISSN: 0014-4827 * |
HARTSHORN C ET AL: "Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos", BMC BIOTECHNOLOGY 20071207 BIOMED CENTRAL LTD.; CURRENT SCIENCE GROUP; MIDDLESEX HOUSE GB, vol. 7, 7 December 2007 (2007-12-07), XP002534226 * |
QUENARD A ET AL: "Deleterious mutations in exon 1 of MECP2 in Rett syndrome", EUROPEAN JOURNAL OF MEDICAL GENETICS, ELSEVIER, NL, vol. 49, no. 4, 1 July 2006 (2006-07-01), pages 313 - 322, XP024920057, ISSN: 1769-7212, [retrieved on 20060701] * |
SANZ EVA ET AL: "Human cord blood CD34+Pax-5+ B-cell progenitors: Single-cell analyses of their gene expression profiles.", BLOOD, vol. 101, no. 9, May 2003 (2003-05-01), pages 3424 - 3430, XP002534227, ISSN: 0006-4971 * |
TANG FUCHOU ET AL: "220-plex microRNA expression profile of a single cell.", NATURE PROTOCOLS 2006, vol. 1, no. 3, 2006, pages 1154 - 1159, XP001539381, ISSN: 1750-2799 * |
TANG FUCHOU ET AL: "MicroRNA expression profiling of single whole embryonic stem cells", NUCLEIC ACIDS RESEARCH, vol. 34, no. 2, 2006, XP002534225, ISSN: 0305-1048 * |
Also Published As
Publication number | Publication date |
---|---|
WO2009087681A2 (en) | 2009-07-16 |
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