WO2009083424A3 - Modified enzymes and their uses - Google Patents
Modified enzymes and their uses Download PDFInfo
- Publication number
- WO2009083424A3 WO2009083424A3 PCT/EP2008/067440 EP2008067440W WO2009083424A3 WO 2009083424 A3 WO2009083424 A3 WO 2009083424A3 EP 2008067440 W EP2008067440 W EP 2008067440W WO 2009083424 A3 WO2009083424 A3 WO 2009083424A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- enzymes
- coupled
- relates
- modified enzymes
- enzyme
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention relates to an active enzyme of bacterial, fungal, viral, or archae origin, wherein the enzyme is coupled, preferably covaiently coupled with at least one polymer having a molecular weight between about 500 to about 20,000 daltons selected from the group consisting of polyethylene glycol and polypropylene glycol. The invention relates in particular to uses thereof of such as in molecular biology as well as kits comprising such enzymes. In preferred embodiments the enzymes of the invention are nucleic acid modifying or replicating enzymes.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP08868070A EP2238245A2 (en) | 2007-12-28 | 2008-12-12 | Modified enzymes and their uses |
US12/810,714 US20110008846A1 (en) | 2007-12-28 | 2008-12-12 | Modified enzymes and their uses |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07150478.1 | 2007-12-28 | ||
EP07150478A EP2075331A1 (en) | 2007-12-28 | 2007-12-28 | Modified enzymes and their uses |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009083424A2 WO2009083424A2 (en) | 2009-07-09 |
WO2009083424A3 true WO2009083424A3 (en) | 2009-09-24 |
Family
ID=39327259
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2008/067440 WO2009083424A2 (en) | 2007-12-28 | 2008-12-12 | Modified enzymes and their uses |
Country Status (3)
Country | Link |
---|---|
US (1) | US20110008846A1 (en) |
EP (2) | EP2075331A1 (en) |
WO (1) | WO2009083424A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110934837B (en) * | 2019-12-16 | 2021-10-22 | 西藏天虹科技股份有限责任公司 | Polygonatum sibiricum polypeptide composite tablet and preparation method thereof |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110250598A1 (en) * | 2010-04-12 | 2011-10-13 | Ulrike Fischer | Detergent free polymerases |
EP3022301B1 (en) | 2013-07-18 | 2018-05-30 | Chr. Hansen A/S | Milk clotting aspartic protease enzyme composition |
CN109112180B (en) * | 2018-09-10 | 2022-07-15 | 山东职业学院 | Method for measuring freeze-thaw times in freezing storage process |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999067371A1 (en) * | 1998-06-24 | 1999-12-29 | Promega Corporation | Enzyme stabilization by cationic surfactants |
WO2008043765A1 (en) * | 2006-10-09 | 2008-04-17 | Qiagen Gmbh | Thermus eggertssonii dna polymerases |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4179337A (en) | 1973-07-20 | 1979-12-18 | Davis Frank F | Non-immunogenic polypeptides |
MX9704137A (en) * | 1994-12-07 | 1997-09-30 | Novo Nordisk As | Polypeptide with reduced allergenicity. |
CN1315997A (en) * | 1998-07-17 | 2001-10-03 | 诺沃奇梅兹有限公司 | Polypeptide-polymer conjugate with improved wash performance |
CA2346929A1 (en) * | 1998-10-13 | 2000-04-20 | Novozymes A/S | A modified polypeptide with reduced immune response |
-
2007
- 2007-12-28 EP EP07150478A patent/EP2075331A1/en not_active Withdrawn
-
2008
- 2008-12-12 US US12/810,714 patent/US20110008846A1/en not_active Abandoned
- 2008-12-12 WO PCT/EP2008/067440 patent/WO2009083424A2/en active Application Filing
- 2008-12-12 EP EP08868070A patent/EP2238245A2/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999067371A1 (en) * | 1998-06-24 | 1999-12-29 | Promega Corporation | Enzyme stabilization by cationic surfactants |
WO2008043765A1 (en) * | 2006-10-09 | 2008-04-17 | Qiagen Gmbh | Thermus eggertssonii dna polymerases |
Non-Patent Citations (8)
Title |
---|
CHRISTAKOPOULOS P ET AL: "Enhancement of pH-stability of a low molecular mass endoglucanase from Fusarium oxysporum by protein pegylation", CARBOHYDRATE RESEARCH, ELSEVIER SCIENTIFIC PUBLISHING COMPANY. AMSTERDAM, NL, vol. 314, no. 1-2, 21 December 1998 (1998-12-21), pages 95 - 99, XP004165165, ISSN: 0008-6215 * |
DOHERTY DANIEL H ET AL: "Site-specific PEGylation of engineered cysteine analogues of recombinant human granulocyte-macrophage colony-stimulating factor", BIOCONJUGATE CHEMISTRY, ACS, WASHINGTON, DC, US, vol. 16, no. 5, 1 September 2005 (2005-09-01), pages 1291 - 1298, XP002453456, ISSN: 1043-1802 * |
LI W ET AL: "Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 905, no. 1-2, 5 January 2001 (2001-01-05), pages 299 - 307, XP004226439, ISSN: 0021-9673 * |
LONG ET AL: "Design of homogeneous, monopegylated erythropoietin analogs with preserved in vitro bioactivity", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 34, no. 6, 1 June 2006 (2006-06-01), pages 697 - 704, XP005504760, ISSN: 0301-472X * |
LONGO M A ET AL: "THERMOSTABILITY OF MODIFIED ENZYMES: A DETAILED STUDY", JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, BLACKWELL SCIENTIFIC PUBLICATIONS. OXFORD, GB, vol. 74, no. 1, 1 January 1999 (1999-01-01), pages 25 - 32, XP000847494, ISSN: 0268-2575 * |
MATSUSHIMA A ET AL: "Bioconjugates of proteins and polyethylene glycol: potent tools in biotechnological processes", JOURNAL OF MOLECULAR CATALYSIS. B, ENZYMATIC, ELSEVIER, AMSTERDAM, NL, vol. 2, 1996, pages 1 - 17, XP002479823, ISSN: 1381-1177 * |
SASAKI YOSHIHARU ET AL: "Effect of molecular crowding on DNA polymerase activity", BIOTECHNOLOGY JOURNAL, vol. 1, no. 4, April 2006 (2006-04-01), pages 440 - 446, XP002490677, ISSN: 1860-6768 * |
XIA Y -M ET AL: "Minimizing the surface effect of PDMS-glass microchip on polymerase chain reaction by dynamic polymer passivation", JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 200701 GB, vol. 82, no. 1, January 2007 (2007-01-01), pages 33 - 38, XP002490676, ISSN: 0268-2575 1097-4660 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110934837B (en) * | 2019-12-16 | 2021-10-22 | 西藏天虹科技股份有限责任公司 | Polygonatum sibiricum polypeptide composite tablet and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
EP2075331A1 (en) | 2009-07-01 |
EP2238245A2 (en) | 2010-10-13 |
US20110008846A1 (en) | 2011-01-13 |
WO2009083424A2 (en) | 2009-07-09 |
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