WO2009063198A3 - Extraction and purification of biological cells using ultrasound - Google Patents

Extraction and purification of biological cells using ultrasound Download PDF

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Publication number
WO2009063198A3
WO2009063198A3 PCT/GB2008/003818 GB2008003818W WO2009063198A3 WO 2009063198 A3 WO2009063198 A3 WO 2009063198A3 GB 2008003818 W GB2008003818 W GB 2008003818W WO 2009063198 A3 WO2009063198 A3 WO 2009063198A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological cells
material sample
ultrasound
purification
extraction
Prior art date
Application number
PCT/GB2008/003818
Other languages
French (fr)
Other versions
WO2009063198A2 (en
Inventor
Damian Joseph Peter Bond
Larisa Alexandrovna Kuznetsova
William Henry Mullen
Carolyn Jennifer Rudell
Paul Birch
Original Assignee
Prokyma Technologies Ltd
Damian Joseph Peter Bond
Larisa Alexandrovna Kuznetsova
William Henry Mullen
Carolyn Jennifer Rudell
Paul Birch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0722300A external-priority patent/GB0722300D0/en
Priority claimed from GB0722776A external-priority patent/GB0722776D0/en
Application filed by Prokyma Technologies Ltd, Damian Joseph Peter Bond, Larisa Alexandrovna Kuznetsova, William Henry Mullen, Carolyn Jennifer Rudell, Paul Birch filed Critical Prokyma Technologies Ltd
Priority to US12/734,671 priority Critical patent/US20100255573A1/en
Priority to EP08850078A priority patent/EP2209545A2/en
Publication of WO2009063198A2 publication Critical patent/WO2009063198A2/en
Publication of WO2009063198A3 publication Critical patent/WO2009063198A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5002—Partitioning blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4094—Concentrating samples by other techniques involving separation of suspended solids using ultrasound

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A process is described in which biological cells are separated from a material sample, such as blood or soil, in which the material sample is formed as a suspension in a fluid and introduced into a chamber. One or more acoustic pressure nodes aggregate the material sample, and a flow of fluid removes soluble matter from the aggregate leaving the biological cells and other inert materials at the node(s). In one aspect of the invention the biological materials are separated from inhibitors that might render their subsequent analysis in, for example, a PCR system difficult. In another aspect of the invention a method is described to extract a clean DNA sample for such biological cells.
PCT/GB2008/003818 2007-11-14 2008-11-14 Extraction and purification of biological cells using ultrasound WO2009063198A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/734,671 US20100255573A1 (en) 2007-11-14 2008-11-14 Extraction and purification of biologigal cells using ultrasound
EP08850078A EP2209545A2 (en) 2007-11-14 2008-11-14 Extraction and purification of biological cells using ultrasound

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0722300.1 2007-11-14
GB0722300A GB0722300D0 (en) 2007-11-14 2007-11-14 Extraction of micro-organisms from a sample
GB0722776.2 2007-11-21
GB0722776A GB0722776D0 (en) 2007-11-21 2007-11-21 Extraction of micro-oganisms

Publications (2)

Publication Number Publication Date
WO2009063198A2 WO2009063198A2 (en) 2009-05-22
WO2009063198A3 true WO2009063198A3 (en) 2009-07-23

Family

ID=40279843

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/003818 WO2009063198A2 (en) 2007-11-14 2008-11-14 Extraction and purification of biological cells using ultrasound

Country Status (3)

Country Link
US (1) US20100255573A1 (en)
EP (1) EP2209545A2 (en)
WO (1) WO2009063198A2 (en)

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US8691145B2 (en) 2009-11-16 2014-04-08 Flodesign Sonics, Inc. Ultrasound and acoustophoresis for water purification
US8956538B2 (en) 2010-06-16 2015-02-17 Flodesign Sonics, Inc. Phononic crystal desalination system and methods of use
US9421553B2 (en) 2010-08-23 2016-08-23 Flodesign Sonics, Inc. High-volume fast separation of multi-phase components in fluid suspensions
US8679338B2 (en) 2010-08-23 2014-03-25 Flodesign Sonics, Inc. Combined acoustic micro filtration and phononic crystal membrane particle separation
WO2012162133A1 (en) 2011-05-20 2012-11-29 Advandx, Inc. Selective ultrasonic lysis of blood and other biological fluids and tissues
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9272234B2 (en) 2012-03-15 2016-03-01 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US9422328B2 (en) 2012-03-15 2016-08-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US10040011B2 (en) 2012-03-15 2018-08-07 Flodesign Sonics, Inc. Acoustophoretic multi-component separation technology platform
US9457302B2 (en) 2014-05-08 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic device with piezoelectric transducer array
US10322949B2 (en) 2012-03-15 2019-06-18 Flodesign Sonics, Inc. Transducer and reflector configurations for an acoustophoretic device
US9752113B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc. Acoustic perfusion devices
US9752114B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc Bioreactor using acoustic standing waves
US9796956B2 (en) 2013-11-06 2017-10-24 Flodesign Sonics, Inc. Multi-stage acoustophoresis device
US10370635B2 (en) 2012-03-15 2019-08-06 Flodesign Sonics, Inc. Acoustic separation of T cells
US9416344B2 (en) 2012-03-15 2016-08-16 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9567559B2 (en) 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9745548B2 (en) 2012-03-15 2017-08-29 Flodesign Sonics, Inc. Acoustic perfusion devices
US10953436B2 (en) 2012-03-15 2021-03-23 Flodesign Sonics, Inc. Acoustophoretic device with piezoelectric transducer array
US9623348B2 (en) 2012-03-15 2017-04-18 Flodesign Sonics, Inc. Reflector for an acoustophoretic device
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US10689609B2 (en) 2012-03-15 2020-06-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US9340435B2 (en) 2012-03-15 2016-05-17 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US9822333B2 (en) 2012-03-15 2017-11-21 Flodesign Sonics, Inc. Acoustic perfusion devices
US9783775B2 (en) 2012-03-15 2017-10-10 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9688958B2 (en) 2012-03-15 2017-06-27 Flodesign Sonics, Inc. Acoustic bioreactor processes
US10737953B2 (en) 2012-04-20 2020-08-11 Flodesign Sonics, Inc. Acoustophoretic method for use in bioreactors
US11324873B2 (en) 2012-04-20 2022-05-10 Flodesign Sonics, Inc. Acoustic blood separation processes and devices
CN106964010A (en) * 2012-04-20 2017-07-21 弗洛设计声能学公司 The sound electrophoretic separation of lipid granule and red blood cell
US9725690B2 (en) 2013-06-24 2017-08-08 Flodesign Sonics, Inc. Fluid dynamic sonic separator
US9745569B2 (en) 2013-09-13 2017-08-29 Flodesign Sonics, Inc. System for generating high concentration factors for low cell density suspensions
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
US9744483B2 (en) 2014-07-02 2017-08-29 Flodesign Sonics, Inc. Large scale acoustic separation device
CN106536732B (en) 2014-07-02 2019-12-31 弗洛设计声能学公司 Acoustophoresis device with uniform fluid flow
WO2016054192A1 (en) 2014-09-30 2016-04-07 Flodesign Sonics, Inc. Acoustophoretic clarification of particle-laden non-flowing fluids
CN106999796B (en) 2014-10-24 2020-09-25 生命科技股份有限公司 Liquid-liquid sample purification system with acoustic precipitation
US10106770B2 (en) 2015-03-24 2018-10-23 Flodesign Sonics, Inc. Methods and apparatus for particle aggregation using acoustic standing waves
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
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EP3297740A1 (en) 2015-05-20 2018-03-28 Flodesign Sonics Inc. Acoustic manipulation of particles in standing wave fields
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US9663756B1 (en) 2016-02-25 2017-05-30 Flodesign Sonics, Inc. Acoustic separation of cellular supporting materials from cultured cells
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JP6861803B2 (en) 2016-09-15 2021-04-21 ラブサイト インコーポレイテッド High-efficiency transfection of biological cells using sonoporation
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Citations (1)

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Patent Citations (1)

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Title
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Publication number Publication date
WO2009063198A2 (en) 2009-05-22
EP2209545A2 (en) 2010-07-28
US20100255573A1 (en) 2010-10-07

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