WO2009054863A2 - Human antibodies that bind cd19 and uses thereof - Google Patents

Human antibodies that bind cd19 and uses thereof Download PDF

Info

Publication number
WO2009054863A2
WO2009054863A2 PCT/US2007/087393 US2007087393W WO2009054863A2 WO 2009054863 A2 WO2009054863 A2 WO 2009054863A2 US 2007087393 W US2007087393 W US 2007087393W WO 2009054863 A2 WO2009054863 A2 WO 2009054863A2
Authority
WO
WIPO (PCT)
Prior art keywords
seq
variable region
chain variable
antibody
amino acid
Prior art date
Application number
PCT/US2007/087393
Other languages
French (fr)
Other versions
WO2009054863A3 (en
Inventor
David John King
Chetana Rao-Naik
Chin Pan
Josephine Cardarelli
Diann Blanset
Original Assignee
Medarex, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medarex, Inc. filed Critical Medarex, Inc.
Priority to BRPI0718349-6A2A priority Critical patent/BRPI0718349A2/en
Priority to EP07875235.9A priority patent/EP2101817A4/en
Priority to MX2009006275A priority patent/MX2009006275A/en
Priority to JP2009541583A priority patent/JP5517626B2/en
Priority to CN200780050552.1A priority patent/CN101636502B/en
Priority to CA002672800A priority patent/CA2672800A1/en
Priority to US12/519,149 priority patent/US20100104509A1/en
Priority to AU2007360636A priority patent/AU2007360636A1/en
Publication of WO2009054863A2 publication Critical patent/WO2009054863A2/en
Publication of WO2009054863A3 publication Critical patent/WO2009054863A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A61K47/6849—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a receptor, a cell surface antigen or a cell surface determinant
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/545—Medicinal preparations containing antigens or antibodies characterised by the dose, timing or administration schedule
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/77—Internalization into the cell
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Definitions

  • CD 19 is a 95 kDa membrane receptor that is expressed early in B cell differentiation and continues to be expressed until the B cells are triggered to terminally differentiate (Pezzutto et al., (1987) J Immunol. 138:2793; Tedder et al. (1994) Immunol Today iJ>:437).
  • the CD 19 extracellular domain contains two C2-type immunoglobulin (IG)-like domains separated by a smaller potentially disulfi de-linked domain.
  • the CD 19 cytoplasmic domain is structurally unique, but highly conserved between human, mouse, and guinea pig (Fujimoto et al., (1998) Semin Immunol. JjO:267).
  • CD 19 is part of a protein complex found on the cell surface of B lymphocytes.
  • the protein complex includes CD 19, CD21 (complement receptor, type 2), CD81 (TAPA-I), and CD225 (Leu- 13) (Fujimoto, supra).
  • CD 19 is an important regulator of transmembrane signals in B cells. An increase or decrease in the cell surface density of CD 19 affects B cell development and function, resulting in diseases such as autoimmunity or hypogammaglobulinemia (Fujimoto, supra).
  • the CD 19 complex potentiates the response of B cells to antigen in vivo through cross-linking of two separate signal transduction complexes found on B cell membranes.
  • CD 19 and B cell receptor cross-linking reduces the number of IgM molecules required to activate PLC (Fujimoto, supra; Ghetie, supra). Additionally, CD 19 functions as a specialized adapter protein for the amplification of Arc family kinases (Hasegawa et al. , (2001 ) J Immunol 167:3190). CD 19 binding has been shown to both enhance and inhibit B-cell activation and proliferation, depending on the amount of cross-linking that occurs (Tedder, supra).
  • CD 19 is expressed on greater than 90% of B-cell lymphomas and has been predicted to affect growth of lymphomas in vitro and in vivo (Ghetie, supra).
  • Antibodies generated to CD 19 have been murine antibodies.
  • a disadvantage of using a murine antibody in treatment of human subjects is the human anti-mouse (HAMA) response on administration to the patient. Accordingly, the need exists for improved therapeutic antibodies against CD 19 which are more effective for treating and/or preventing diseases mediated by CD 19.
  • the present disclosure provides isolated monoclonal antibodies, in particular human monoclonal antibodies, that specifically bind to CD 19 and that exhibit numerous desirable properties. These properties include high affinity binding to human CDl 9, internalization by cells expressing CD 19, and/or the ability to mediate antigen depu-uci > cellular cytotoxicity.
  • the antibodies of the invention can be used, for example, to detect CD 19 protein or to inhibit the growth of cells expressing CD 19, such as tumor cells that express CD 19. Also provided are methods for treating a variety CD 19 mediated diseases using the antibodies and compositions of this disclosure.
  • this disclosure pertains to an isolated monoclonal human or an antigen binding portion thereof, wherein the antibody binds human CD 19 and exhibits at least one of the following properties: (a) binds to human CD 19 with a K 0 of 1 x 10 "7 M or less;
  • ADCC antibody dependent cellular cytotoxicity
  • the antibody exhibits at least two of properties (a), (b), (c), (d), and (e). More preferably, the antibody exhibits at least three of properties (a), (b), (c), (d), and (e). More preferably, the antibody exhibits four of properties (a), (b), (c), (d), and (e). Even, more preferably, the antibody exhibits all five of properties (a), (b), (c), (d),
  • the antibody inhibits growth of CDl 9- expressing tumor cells in vivo when the antibody is conjugated to a cytotoxin.
  • the antibody binds to human CD 19 with a Kp of 5 x 10 ⁇ 8 M or less, binds to human CD 19 with a K D of 2 x 10 "8 M or less, binds to human CD 19 with a K D of 1 x 10 "8 M or less, binds to human CD 19 with a K D of 5x 10 ⁇ 9 M or less, binds to human CD 19 with a K D of 4x10 "9 M or less, binds to human CD 19 with a KQ of 3xlO "9 M or less, or binds to human CD 19 with a K 0 of 2 x 10 ⁇ 9 M or less.
  • the antibody is a human antibody, although in alternative embodiments the antibody can be a murine antibody, a chimeric antibody or humanized antibody.
  • the invention pertains to an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody cross-competes for binding to an epitope on human CD 19 which is recognized by a reference antibody, wherein the reference antibody comprises:
  • the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 ; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 9; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 2; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 3; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 1 1 ; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4; and (b) a light chain variable region compris
  • this disclosure pertains to an isolated monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a heavy chain variable region that is the product of or derived from a human V H 5-51 gene, wherein the antibody specifically binds CD 19.
  • This disclosure also provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a heavy chain variable region that is the product of or derived from a human V H 1-69 gene, wherein the antibody specifically binds CD 19.
  • This disclosure still further provides an isolated human monoclonal antibody, or antigen binding portion thereof comprising a light chain variable region that is the product of or derived from a human V ⁇ Ll 8 gene, wherein the antibody specifically binds CD 19.
  • This disclosure even further provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a light chain variable region that is the product of or derived from a human V K A27 gene, wherein the antibody specifically binds CD 19.
  • This disclosure even further provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a light chain variable region that is the product of or derived from a human V K L15 gene, wherein the antibody specifically binds CD19.
  • this disclosure provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises (a) a heavy chain variable region of a human V H 5-51 or 1-69 gene; and (b) a light chain variable region of a human V ⁇ Ll 8, A27 or V K L 15; wherein the antibody specifically binds to CD 19.
  • this disclosure provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a heavy chain variable region that comprises CDRl, CDR2, and CDR3 sequences; and a light chain variable region that comprises CDRl, CDR2, and CDR3 sequences, wherein: (a) the heavy chain variable region CDR3 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36, and conservative modifications thereof; (b) the light chain variable region CDR3 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequence of SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58, and conservative modifications thereof; (c) the antibody binds to human CD 19 with a Kp of IxI(T 7 M or less; and (d) binds to Raji and Daudi B-cell tumor cells.
  • the antibody comprises a heavy chain variable region that comprises CDRl, CDR2, and CDR3 sequences
  • the heavy chain variable region CDR2 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, and conservative modifications thereof; and the light chain variable region CDR2 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50, and conservative modifications thereof.
  • the heavy chain variable region CDRl sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22, and conservative modifications thereof; and the light chain variable region CDRl sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43, and conservative modifications thereof.
  • a preferred combination comprises: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 16;
  • Another preferred combination comprises:
  • a light chain variable region CDR3 comprising SEQ ID NO: 53.
  • Another preferred combination comprises: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 18;
  • Another preferred combination comprises:
  • Another preferred combination comprises:
  • a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6 and 7;
  • a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 9, 10, 1 1, 12, 13, 14 and 15; wherein the antibody specifically binds CD 19.
  • a preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8.
  • Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 ; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 9.
  • Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 2; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10.
  • Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 3; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 1 1.
  • Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12.
  • Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 5; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13.
  • Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14.
  • Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15.
  • antibodies, or antigen-binding portion or fragments thereof are provided that compete for binding to CD 19 with any of the aforementioned antibodies.
  • the antibodies of this disclosure can be, for example, full-length antibodies, for example of an IgGl or IgG4 isotype.
  • the antibodies can be antibody fragments, such as Fab, Fab' or Fab'2 fragments, or single chain antibodies.
  • This disclosure also provides an immunoconjugate comprising an antibody of this disclosure, or antigen-binding portion thereof, linked to a therapeutic agent, such as a cytotoxin or a radioactive isotope.
  • a therapeutic agent such as a cytotoxin or a radioactive isotope.
  • the invention provides an immunoconjugate comprising an antibody of this disclosure, or antigen-binding portion thereof, linked to a cytotoxin (for example, a cytotoxin described herein or in U.S. Pat. App. No. 60/882,461, filed on December 28, 2006 or U.S. Pat. App. No. 60/991,300, filed on November 30, 2007, which are hereby incorporated by reference in their entirety) (e.g., via a thiol linkage).
  • a cytotoxin for example, a cytotoxin described herein or in U.S. Pat. App. No. 60/882,461, filed on December 28, 2006 or U.S. Pat. App. No. 60/991,300, filed on November 30, 2007, which are hereby incorporated by reference in their entirety
  • the invention provides the following preferred immunoconjugates:
  • an immunoconjugate comprising an antibody, or antigen-binding portion thereof, comprising: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8.
  • an immunoconjugate comprising an antibody, or antigen-binding portion thereof, comprising:
  • a light chain variable region CDR3 comprising SEQ ID NO: 51; an antibody, or antigen-binding portion thereof, comprising:
  • a light chain variable region CDR3 comprising SEQ ID NO: 52; an antibody, or antigen-binding portion thereof, comprising:
  • a light chain variable region CDR3 comprising SEQ ID NO: 53; an antibody, or antigen-binding portion thereof, comprising:
  • a light chain variable region CDR3 comprising SEQ ID NO: 54; an antibody, or antigen-binding portion thereof, comprising: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 19;
  • a light chain variable region CDR2 comprising SEQ ID NO: 47
  • a light chain variable region CDR3 comprising SEQ ID NO: 55
  • an antibody, or antigen-binding portion thereof comprising: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 20;
  • a light chain variable region CDR3 comprising SEQ ID NO: 56; an antibody, or antigen-binding portion thereof, comprising:
  • a light chain variable region CDR3 comprising SEQ ID NO: 57; or an antibody, or antigen-binding portion thereof, comprising: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 22;
  • an immunoconjugate comprising an antibody, or antigen-binding portion thereof, that binds to the same epitope that is recognized by (e.g., cross-competes for binding to human CD 19 with) an antibody comprising: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:
  • a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15, linked to a cytotoxin.
  • This disclosure also provides a bispecific molecule comprising an antibody, or antigen-binding portion or fragment thereof, of this disclosure, linked to a second functional moiety having a different binding specificity than said antibody, or antigen binding portion thereof.
  • compositions comprising an antibody, or antigen-binding portion thereof, or immunoconjugate or bispecific molecule of this disclosure and a pharmaceutically acceptable carrier are also provided.
  • Nucleic acid molecules encoding the antibodies, or antigen-binding portions thereof, of this disclosure are also encompassed by this disclosure, as well as expression vectors comprising such nucleic acids and host cells comprising such expression vectors.
  • Methods for preparing anti-CD 19 antibodies using the host cells comprising such expression vectors are also provided and may include the steps of (i) expressing the antibody in the host cell and (ii) isolating the antibody from the host cell.
  • the invention pertains to a method for preparing an anti- CD 19 antibody.
  • the method comprises: (a) providing: (i) a heavy chain variable region antibody sequence comprising a
  • the present disclosure also provides isolated anti-CD 19 antibody-partner molecule conjugates that specifically bind to CD 19 with high affinity, particularly those comprising human monoclonal antibodies. Certain of such antibody-partner molecule conjugates are capable of being internalized into CD19-expressing cells and are capable of mediating antigen dependent cellular cytotoxicity. This disclosure also provides methods for treating cancers, such as treat B cell malignancies, including non-Hodgkin's lymphoma, chronic lymphocytic leukemias, follicular lymphomas, diffuse large cell lymphomas of B lineage, and multiple myelomas, using an anti-CD 19 antibody-partner molecule conjugate disclosed herein.
  • compositions comprising an antibody, or antigen-binding portion thereof, conjugated to a partner molecule of this disclosure are also provided.
  • Partner molecules that can be advantageously conjugated to an antibody in an antibody partner molecule conjugate as disclosed herein include, but are not limited to, molecules as drugs, cytotoxins, marker molecules (e.g., radioisotopes), proteins and therapeutic agents.
  • Compositions comprising antibody-partner molecule conjugates and pharmaceutically acceptable earners are also disclosed herein.
  • such antibody-partner molecule conjugates are conjugated via chemical linkers.
  • the linker is a peptidyl linker, and is depicted herein as (L 4 ) p — F — (L 1 ), thinking.
  • Other linkers include hydrazine and disulfide linkers, and is depicted herein as (L 4 ) p — H — (L') m or (L 4 ) p — J — (L') m , respectively.
  • the present invention also provides cleavable linker arms that are appropriate for attachment to essentially any molecular species.
  • the invention pertains to a method of inhibiting growth of a CD 19-expressing tumor cell.
  • the method comprises contacting the CD 19-expressing tumor cell with an antibody-partner molecule conjugate of the disclosure such that growth of the CDl 9-expressing tumor cell is inhibited.
  • the partner molecule is a therapeutic agent, such as a cytotoxin.
  • Particularly preferred CD 19- expressing tumor cells are B-cell tumor cells.
  • the invention pertains to a method of treating cancer in a subject. The method comprises administering to the subject an antibody-partner molecule conjugate of the disclosure such that the cancer is treated in the subject.
  • the partner molecule is a therapeutic agent, such as a cytotoxin.
  • Particularly preferred cancers for treatment are B cell malignancies, for example, non- Hodgkin's lymphoma, chronic lymphocytic leukemias, follicular lymphomas, diffuse large cell lymphomas of B lineage, and multiple myelomas.
  • B cell malignancies for example, non- Hodgkin's lymphoma, chronic lymphocytic leukemias, follicular lymphomas, diffuse large cell lymphomas of B lineage, and multiple myelomas.
  • Figure IA shows the nucleotide sequence (SEQ ID NO: 59) and amino acid sequence (SEQ ID NO: 1) of the heavy chain variable region of the 21D4 and 21 D4a human monoclonal antibodies.
  • the CDRl (SEQ ID NO: 16), CDR2 (SEQ ID NO: 23) and CDR3 (SEQ ID NO: 30) regions are delineated and the V, D and J germline derivations are indicated.
  • Figure IB shows the nucleotide sequence (SEQ ID NO: 66) and amino acid sequence (SEQ ID NO: 8) of the light chain variable region of the 21D4 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 37), CDR2 (SEQ ID NO: 44) and CDR3 (SEQ ID NO: 51) regions are delineated and the V and J germline derivations are indicated.
  • Figure 1 C shows the nucleotide sequence (SEQ ID NO: 67) and amino acid sequence (SEQ ID NO: 9) of the light chain variable region of the 21D4a human monoclonal antibody.
  • the CDRl (SEQ ID NO: 37), CDR2 (SEQ ID NO: 44) and CDR3 (SEQ ID NO: 52) regions are delineated and the V and J germline derivations are indicated.
  • Figure 2A shows the nucleotide sequence (SEQ ID NO: 60) and amino acid sequence (SEQ ID NO: 2) of the heavy chain variable region of the 47G4 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 17), CDR2 (SEQ ID NO: 24) and CDR3 (SEQ ID NO: 31) regions are delineated and the V, D and J germline derivations are indicated.
  • Figure 2B shows the nucleotide sequence (SEQ ID NO: 68) and amino acid sequence (SEQ ID NO: 10) of the light chain variable region of the 47G4 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 38), CDR2 (SEQ ID NO: 45) and CDR3 (SEQ ID NO: 53) regions are delineated and the V and J germline derivations are indicated.
  • Figure 3 A shows the nucleotide sequence (SEQ ID NO: 61) and amino acid sequence (SEQ ID NO: 3) of the heavy chain variable region of the 27F3 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 18), CDR2 (SEQ ID NO: 25) and CDR3 (SEQ ID NO: 32) regions are delineated and the V, D and J germline derivations are indicated.
  • Figure 3B shows the nucleotide sequence (SEQ ID NO: 69) and amino acid sequence (SEQ ID NO: 11) of the light chain variable region of the 27F3 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 39), CDR2 (SEQ ID NO: 46) and CDR3 (SEQ ID NO: 54) regions are delineated and the V and J germline derivations are indicated.
  • Figure 4A shows the nucleotide sequence (SEQ ID NO: 62) and amino acid sequence (SEQ ID NO: 4) of the heavy chain variable region of the 3C10 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 19), CDR2 (SEQ ID NO: 26) and CDR3 (SEQ ID NO: 33) regions are delineated and the V, D and J germline derivations are indicated.
  • Figure 4B shows the nucleotide sequence (SEQ ID NO: 70) and amino acid sequence (SEQ ID NO: 12) of the light chain variable region of the 3C10 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 40), CDR2 (SEQ ID NO: 47) and CDR3 (SEQ ID NO: 55) regions are delineated and the V and J germline derivations are indicated.
  • Figure 5A shows the nucleotide sequence (SEQ ID NO: 63) and amino acid sequence (SEQ ID NO: 5) of the heavy chain variable region of the 5G7 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 20), CDR2 (SEQ ID NO: 27) and CDR3 (SEQ ID NO: 34) regions are delineated and the V, D and J ge ⁇ nline derivations are indicated.
  • Figure 5B shows the nucleotide sequence (SEQ ID NO: 71) and amino acid sequence (SEQ ID NO: 13) of the light chain variable region of the 5G7 human monoclonal antibody.
  • the CDRl (SEQ ID NO: 41), CDR2 (SEQ ID NO: 48) and CDR3 (SEQ ID NO: 56) regions are delineated and the V and J ge ⁇ nline derivations are indicated.
  • Figure 6A shows the nucleotide sequence (SEQ ID NO: 64) and amino acid sequence (SEQ ID NO: 6) of the heavy chain variable region of the 13Fl human monoclonal antibody.
  • the CDRl (SEQ ID NO: 21), CDR2 (SEQ ID NO: 28) and CDR3 (SEQ ID NO: 35) regions are delineated and the V, D and J germline derivations are indicated.
  • Figure 6B shows the nucleotide sequence (SEQ ID NO: 72) and amino acid sequence (SEQ ID NO: 14) of the light chain variable region of the 13Fl human monoclonal antibody.
  • the CDRl (SEQ ID NO: 42), CDR2 (SEQ ID NO: 49) and CDR3 (SEQ ID NO: 57) regions are delineated and the V and J germline derivations are indicated.
  • Figure 7 A shows the nucleotide sequence (SEQ ID NO: 65) and amino acid sequence (SEQ ID NO: 7) of the heavy chain variable region of the 46E8 human monoclonal antibody.
  • the CDRl SEQ ID NO: 22
  • CDR2 SEQ ID NO: 29
  • CDR3 (SEQ ID NO: 36) regions are delineated and the V, D and J germiine derivations are indicated.
  • Figure 7B shows the nucleotide sequence (SEQ ID NO: 73) and amino acid sequence (SEQ ID NO: 15) of the light chain variable region of the 46E8 human monoclonal antibody.
  • the CDRl SEQ ID NO: 43
  • CDR2 SEQ ID NO: 50
  • CDR3 (SEQ ID NO: 58) regions are delineated and the V and J ge ⁇ nline derivations are indicated.
  • Figure 8 shows the alignment of the amino acid sequence of the heavy chain variable region of 21D4 (SEQ ID NO: 1) and 21D4a (SEQ ID NO: 1), with the human ge ⁇ nline V H 5-51 amino acid sequence (SEQ ID NO: 74).
  • the JH4b germline is disclosed as SEQ ID NO: 80.
  • Figure 9 shows the alignment of the amino acid sequence of the heavy chain variable region of 47G4 (SEQ ID NO: 2) with the human ge ⁇ nline Vn 1-69 amino acid sequences (SEQ ID NO: 75).
  • the JH5b germline is disclosed as SEQ ID NO: 81.
  • Figure 10 shows the alignment of the amino acid sequence of the heavy chain variable region of 27F3 (SEQ ID NO: 3), with the human germline Vn 5-51 amino acid sequence (SEQ ID NO: 74).
  • the JH6b ge ⁇ nline is disclosed as SEQ ID NO: 82.
  • Figure 11 shows the alignment of the amino acid sequence of the heavy chain variable region of 3C10 (SEQ ID NO: 4) with the human germline V H 1-69 amino acid sequences (SEQ ID NO: 75).
  • the JH6b germline is disclosed as SEQ ID NO: 82.
  • Figure 12 shows the alignment of the amino acid sequence of the heavy chain variable region of 5G7 (SEQ ID NO: 5), with the human ge ⁇ nline V H 5-51 amino acid sequence (SEQ ID NO: 74).
  • the JH6b ge ⁇ nline is disclosed as SEQ ID NO: 83.
  • Figure 13 shows the alignment of the amino acid sequence of the heavy chain variable region of 13Fl (SEQ ID NO: 6), with the human germline V H 5-51 amino acid sequence (SEQ ID NO: 74).
  • the JH6b ge ⁇ nline is disclosed as SEQ ID NO: 82.
  • Figure 14 shows the alignment of the amino acid sequence of the heavy chain variable region of 46E8 (SEQ ID NO: 7), with the human germline V H 5-51 amino acid sequence (SEQ ID NO: 74).
  • the JH6b germline is disclosed as SEQ ID NO: 82.
  • Figure 15 shows the alignment of the amino acid sequence of the light chain variable region of 21D4 (SEQ ID NO: 8) with the human germline V k L18 amino acid sequence (SEQ ID NO:76).
  • the JK2 ge ⁇ nline is disclosed as SEQ ID NO: 84.
  • Figure 16 shows the alignment of the amino acid sequence of the light chain variable region of 21D4a (SEQ ID NO: 9) with the human ge ⁇ nline Vt- LlS amino acid sequence (SEQ ID NO:76).
  • the JK3 germline is disclosed as SEQ ID NO: 85.
  • Figure 17 shows the alignment of the amino acid sequence of the light chain variable region of 47G4 (SEQ ID NO: 10) with the human germline V k A27 amino acid sequence (SEQ ID NO:77).
  • the JK3 ge ⁇ nline is disclosed as SEQ ID NO: 85.
  • Figure 18 shows the alignment of the amino acid sequence of the light chain variable region of 27F3 (SEQ ID NO: 1 1 ) with the human germline V k L18 amino acid sequence (SEQ ID NO:76).
  • the JK2 germline is disclosed as SEQ ID NO: 84.
  • Figure 19 shows the alignment of the amino acid sequence of the light chain variable region of 3C10 (SEQ ID NO: 12) with the human germline Vk L15 amino acid sequence (SEQ ID NO:78).
  • the JK2 germline is disclosed as SEQ ID NO: 84.
  • Figure 20 shows the alignment of the amino acid sequence of the light chain variable region of 5G7 (SEQ ID NO: 13) with the human germline V k Ll 8 amino acid sequence (SEQ ID NO:76).
  • the JKl ge ⁇ nline is disclosed as SEQ ID NO: 86.
  • Figure 21 shows the alignment of the amino acid sequence of the light chain variable region of 13Fl (SEQ ID NO: 14) with the human germline V k Ll 8 amino acid sequence (SEQ ID NO:76).
  • the JK2 ge ⁇ nline is disclosed as SEQ ID NO: 87.
  • Figure 22 shows the alignment of the amino acid sequence of the light chain variable region of 46E8 (SEQ ID NO: 15) with the human germline V k Ll 8 amino acid sequence (SEQ ID NO:76).
  • the JK2 germline is disclosed as SEQ ID NO: 87.
  • Figure 23 is a graph showing the results of experiments demonstrating that the human monoclonal antibody 47 G4, directed against human CD 19, specifically binds to human CD 19.
  • Figure 24 A and B are graphs showing the results of experiments demonstrating that the human monoclonal antibodies against CD 19 compete for binding on Raji cells.
  • Figure 25A-D shows the results of flow cytometry experiments demonstrating that the human monoclonal antibodies 21 D4, 21 D4a, 47G4, 3Cl 0, 5G7 and 13F 1 , directed against human CD 19, binds the cell surface of B-cell tumor cell lines.
  • A Flow cytometry of HuMAbs 21D4 and 47G4 on CHO cells transfected with human CD 19.
  • B Flow cytometry of HuMAb 47G4 on Daudi B tumor cells.
  • C Flow cytometry of HuMAbs 21 D4 and 47G4 on Raji B tumor cells.
  • D Flow cytometry of HuMAbs 21 D4, 21D4a, 3C10, 5G7 and 13Fl on Raji B tumor cells.
  • Figures 26A-B shows the results of internalization experiments demonstrating that the human monoclonal antibodies 21D4 and 47G4, directed against human CD 19, enters CHO-CD 19 and CD19-expressing Raji B tumor cells by a 3H-thymidine release assay.
  • HuMAb 47G4 internalization into CHO-CD 19 cells.
  • Figure 27A and B shows the results of a thymidine incorporation assay demonstrating that human monoclonal antibodies directed against human CD 19 kill Raji B cell tumor cells.
  • Figure 28 shows a Kaplan-Meier plot of mouse survival in a Ramos systemic model.
  • Figure 29A-B shows the body weight change in mice in a Ramos systemic model.
  • Figure 30A-B shows the results of an in vivo mouse tumor model study demonstrating that treatment with naked anti-CD 19 antibody 21D4 has a direct inhibitory effect on lymphoma tumors in vivo.
  • A ARH-77 tumors
  • B Raji tumors.
  • Figure 31 shows the results of an antibody dependent cellular cytotoxicity (ADCC) assay demonstrating that nonfucosylated human monoclonal anti-CD 19 antibodies have increased cell cytotoxicity on human leukemia cells in an ADCC dependent manner.
  • ADCC antibody dependent cellular cytotoxicity
  • Figure 32 shows the results of an in vivo mouse tumor model study demonstrating that cytotoxin-conjugated anti-CD19 antibodies reduce tumor volume.
  • Toxin 1 is cytotoxin Nl and toxin 2 is cytotoxin N2.
  • Figure 33 shows the body weight change in mice in a Raji tumor model study. Toxin 1 is cytotoxin Nl and toxin 2 is cytotoxin N2.
  • Figure 34 shows the results of a cynomolgus monkey study showing a decreased population of CD20+ cells following treatment of iucosylated or nonfucosylated anti- CD 19 HuMAbS.
  • Figure 35 shows the results of individual cynomolgus monkeys following treatment with fucosylated or nonfucosylated anti-CD 19 HuMAbs.
  • Figure 36A-C shows the results of a thymidine incoiporation assay demonstrating that human monoclonal antibodies directed against human CD 19 alone or cytotoxin- conjugated kill Raji and SU-DHL-6 B cell tumor cells.
  • Figure 37 shows the in vivo efficacy of immunoconjugate anti-CD 19-N2 against tumor formation in a subcutaneous xenograft SCID mouse model.
  • Figure 38 shows the in vivo efficacy of immunoconjugate anti-CD 19-N2 against tumor formation in a subcutaneous Burkitt's lymphoma SCID mouse model.
  • Figure 39 shows the in vivo efficacy of immunoconjugate anti-CD 19-N2 against tumor formation in a systemic SCID mouse model.
  • Figure 4OA shows that B cells (CD20 + ) were decreased in a dose-dependent manner after administration of 21D4 with minimal or no depletion at 0.01 mg/kg. B cells decreased to 16% to 32% of baseline after administration of 0.1 mg/kg.
  • Figure 4OB illustrates that the magnitude and length of B-cell depletion after administration of 21D4 was similar to that of a 0.1 mg/kg injection of rituximab.
  • Figure 41 shows the in vivo efficacy of a single dose of anti-CD 19-cytotoxin A against tumor formation in a Raji xenograft SCID mouse model.
  • Figure 42 shows the in vivo efficacy of a single dose of anti-CD 19-cytotoxin A against tumor formation in a Raji xenograft SClD mouse model, including an isotype control.
  • Figure 43 shows the in vivo efficacy of a single dose and repeat doses of anti- CD 19-cytotoxin A against tumor formation in a Ramos xenograft Es l e nude mouse model.
  • Figure 44 shows the in vivo efficacy of a single dose of anti-CD 19-cytotoxin A against tumor formation in a Daudi xenograft SCID mouse model.
  • Figure 45 shows the in vivo efficacy of a single dose of anti-CD 19-N2 against tumor formation in a SU-DHL6 xenograft SClD mouse model.
  • N2 cytotoxin B.
  • Figure 46 is the structure of cytotoxin A.
  • the present disclosure relates to isolated monoclonal antibodies, particularly human monoclonal antibodies which bind specifically to human' CD 19 with high affinity and that have desirable functional properties.
  • the antibodies of this disclosure are derived from particular heavy and light chain germline sequences and/or comprise particular structural features such as CDR regions comprising particular amino acid sequences.
  • This disclosure provides isolated antibodies, methods of making such antibodies, antibody-partner molecule conjugates, and bispecific molecules comprising such antibodies and pharmaceutical compositions containing the antibodies, antibody-partner molecule conjugates or bispecific molecules of this disclosure.
  • This disclosure also relates to methods of using the antibodies, such as to detect CD 19, as well as to treat diseases associated with expression of CD19, such as B cell malignancies that express CD 19.
  • this disclosure also provides methods of using the anti- CD 19 antibodies and antibody-partner molecule conjugates of this disclosure to treat B cell malignancies, for example, in the treatment of non-Hodgkin " s lymphoma, chronic lymphocytic leukemias, follicular lymphomas, diffuse large cell lymphomas of B lineage, and multiple myelomas.
  • CD 19 refers to, for example, variants, iso forms, homologs, orthologs and paralogs of human CD 19. Accordingly, human antibodies of this disclosure may, in certain cases, cross-react with CD 19 from species other than human. In certain embodiments, the antibodies may be completely specific for one or more human CD 19 proteins and may not exhibit species or other types of non-human cross-reactivity, or may cross-react with CD 19 from certain other species but not all other species (e.g., cross-react with a primate CD 19 but not mouse CD 19).
  • human CD 19 refers to human sequence CD 19, such as the complete amino acid sequence of human CD 19 having Genbank Accession Number NM_001770 (SEQ ID NO: 79).
  • mouse CD 19 refers to mouse sequence CD 19, such as the complete amino acid sequence of mouse CD 19 having Genbank Accession Number AAA37390.
  • the human CD 19 sequence may differ from human CD 19 of Genbank Accession Number NM_001770 by having, for example, conserved mutations or mutations in non- conserved regions and the CD 19 has substantially the same biological function as the human CD 19 of Genbank Accession Number NMJ301770.
  • a particular human CD 19 sequence will generally be at least 90% identical in amino acids sequence to human CD 19 of Genbank Accession Number NIvIJ)01770 and contains amino acid residues that identify the amino acid sequence as being human when compared to CD 19 amino acid sequences of other species (e.g., murine).
  • a human CD 19 may be at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to CD 19 of Genbank Accession Number NM_001770.
  • a human CD 19 sequence will display no more than 10 amino acid differences from the CD 19 sequence of Genbank Accession Number NM_001770.
  • the human CD 19 may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the CD 19 sequence of Genbank Accession Number NMJ301770. Percent identity can be determined as described herein.
  • immune response refers to the action of, for example, lymphocytes, antigen presenting cells, phagocytic cells, granulocytes, and soluble macromolecules produced by the above cells or the liver (including antibodies, cytokines, and complement) that results in selective damage to, destruction of, or elimination from the human body of invading pathogens, cells or tissues infected with pathogens, cancerous cells, or, in cases of autoimmunity or pathological inflammation, normal human cells or tissues.
  • a “signal transduction pathway” refers to the biochemical relationship between a variety of signal transduction molecules that play a role in the transmission of a signal from one portion of a cell to another portion of a cell.
  • the phrase “cell surface receptor” includes, for example, molecules and complexes of molecules capable of receiving a signal and the transmission of such a signal across the plasma membrane of
  • a cell An example of a "cell surface receptor" of the present disclosure is the CD 19 receptor.
  • antibody' * as referred to herein includes whole antibodies and any antigen binding fragment (i.e., "antigen-binding portion") or single chains thereof.
  • An “antibody” refers to a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds, or an antigen binding portion thereof.
  • Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as V H ) and a heavy chain constant region.
  • the heavy chain constant region is comprised of three domains, C H I , C H 2 and C H 3.
  • Each light chain is comprised of a light chain variable region (abbreviated herein as V L ) and a light chain constant region.
  • the light chain constant region is comprised of one domain, C L .
  • the V H and V L regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR).
  • CDR complementarity determining regions
  • FR framework regions
  • Each V H and V L is composed of three CDRs and four FRs, arranged from amino- terminus to carboxy-te ⁇ ninus in the following order: FRl, CDRl, FR2, CDR2, FR3,
  • variable regions of the heavy and light chains contain a binding domain that interacts with an antigen.
  • the constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (CIq) of the classical complement system.
  • antibody fragment ' ' and "antigen-binding portion" of an antibody refer to one or more fragments of an antibody that retain the ability to specifically bind to an antigen (eg., CD 19). It has been shown that the antigen-binding function of an antibody can be performed by fragments of a full- length antibody.
  • binding fragments encompassed within the term "antigen- binding portion" of an antibody include (i) a Fab fragment, a monovalent fragment consisting of the V L , VH, C 1 and C H I domains; (ii) a F(ab')2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fab 1 fragment, which is essentially an Fab with part of the hinge region (see, FUNDAMENTAL IMMUNOLOGY, Paul ed., 3rd ed. 1993); (iv) a Fd fragment consisting of the V H and Ci
  • -21- domains of a single arm of an antibody (vi) a dAb fragment (Ward et al., (1989) Nature 341:544-546), which consists of a V n domain; (vii) an isolated complementarity determining region (CDR); and (viii) a nanobody, a heavy chain variable region containing a single variable domain and two constant domains.
  • a dAb fragment Ward et al., (1989) Nature 341:544-546
  • CDR complementarity determining region
  • nanobody a heavy chain variable region containing a single variable domain and two constant domains.
  • the two domains of the Fv fragment, V 1 and Vn are coded for by separate genes, they can be joined, using recombinant methods, by a synthetic linker that enables them to be made as a single protein chain in which the V L and V H regions pair to form monovalent molecules (known as single chain Fv (scFv); see e.g., Bird et al. (1988) Science 242:423- 426; and Huston et al. (1988) Proc. Natl. Acad. ScL USA 85:5879-5883).
  • single chain Fv single chain Fv
  • Such single chain antibodies are also intended to be encompassed within the term "antigen-binding portion" of an antibody.
  • an "isolated antibody”, as used herein, is intended to refer to an antibody that is substantially free of other antibodies having different antigenic specificities (e.g., an isolated antibody that specifically binds CD 19 is substantially free of antibodies that specifically bind antigens other than CD 19).
  • An isolated antibody that specifically binds CD 19 may, however, have cross-reactivity to other antigens, such as CD 19 molecules from other species.
  • an isolated antibody may be substantially free of other cellular material and/or chemicals.
  • the terms “monoclonal antibody” or “monoclonal antibody composition” as used herein refer to a preparation of antibody molecules of single molecular composition.
  • a monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope.
  • the term "human antibody”, as used herein, is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from human germline immunoglobulin sequences. Furthermore, if the antibody contains a constant region, the constant region also is derived from human germline immunoglobulin sequences.
  • the human antibodies of this disclosure may include amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo).
  • the tenn "human antibody”, as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences.
  • the tenn "human monoclonal antibody” refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human germline immunoglobulin sequences.
  • the human monoclonal antibodies are produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell.
  • recombinant human antibody includes all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as (a) antibodies isolated from an animal ⁇ e.g., a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom (described further below), (b) antibodies isolated from a host cell transformed to express the human antibody, e.g., from a transfectoma, (c) antibodies isolated from a recombinant, combinatorial human antibody library, and (d) antibodies prepared, expressed, created or isolated by any other means that involve splicing of human immunoglobulin gene sequences to other DNA sequences.
  • recombinant means such as (a) antibodies isolated from an animal ⁇ e.g., a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom (described further below), (b) antibodies isolated from a host cell transformed to express the human antibody, e.
  • Such recombinant human antibodies have variable regions in which the framework and CDR regions are derived from human germline immunoglobulin sequences.
  • such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the V H and V ( regions of the recombinant antibodies are sequences that, while derived from and related to human germline V H and Vi sequences, may not naturally exist within the human antibody germline repertoire in vivo.
  • isotype refers to the antibody class (e.g., IgM or IgGl) that is encoded by the heavy chain constant region genes.
  • the phrases “an antibody recognizing an antigen” and “an antibody specific for an antigen” are used interchangeably herein with the term “an antibody which binds specifically to an antigen.”
  • human antibody derivatives refers to any modified form of the human antibody, e.g., a conjugate of the antibody and another agent or antibody.
  • humanized antibody is intended to refer to antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences. Additional framework region modifications may be made within the human framework sequences.
  • chimeric antibody is intended to refer to antibodies in which the variable region sequences are derived from one species and the constant region sequences are derived from another species, such as an antibody in which the variable region sequences are derived from a mouse antibody and the constant region sequences are derived from a human antibody.
  • antibody mimetic is intended to refer to molecules capable of mimicking an antibody's ability to bind an antigen, but which are not limited to native antibody structures.
  • antibody mimetics include, but are not limited to, Aff ⁇ bodies, DARPins, Anticalins, Avimers, and Versabodies, all of which employ binding structures that, while they mimic traditional antibody binding, are generated from and function via distinct mechanisms.
  • partner molecule refers to the entity which is conjugated to an antibody in an antibody-partner molecule conjugate.
  • partner molecules include drags, cytotoxins, marker molecules (including, but not limited to peptide and small molecule markers such as fluorochrome markers, as well as single atom markers such as radioisotopes), proteins and therapeutic agents.
  • an antibody that "specifically binds to human CD 19" is intended to refer to an antibody that binds to human CD 19 with a K D of I x 10 "7 M or less, more preferably 5 x 1O *8 M or less, more preferably 3 x 10 s M or less, more preferably 1 x 1(T 8 M or less, even more preferably 5 x 10 "9 M or less.
  • the term "does not substantially bind" to a protein or cells means does not bind or does not bind with a high affinity to the protein or cells, i.e.
  • K assoc or "K a ", as used herein, is intended to refer to the association rate of a particular antibody-antigen interaction
  • K dis or "'K d ,” as used herein, is intended to refer to the dissociation rate of a particular antibody- antigen interaction
  • 'K D is intended to refer to the dissociation constant, which is obtained from the ratio of K d to K a (i.e., K d /K a ) and is expressed as a molar concentration (M).
  • K D values for antibodies can be determined using methods well established in the art.
  • a preferred method for determining the KQ of an antibody is by using surface plasmon resonance, preferably using a biosensor system such as a Biacore" system.
  • the term ''high affinity" for an IgG antibody refers to an antibody having a Kp of 1 x 10 "7 M or less, more preferably 5 x 10 "8 M or less and even more preferably 1 x 10 "9 M or less and even more preferably 5 x 10 "9 M or less for a target antigen.
  • ''high affinity” binding can vary for other antibody isotypes.
  • "high affinity” binding for an IgM isotype refers to an antibody having a K D of 10 "6 M or less, more preferably 10 "7 M or less, even more preferably 10 s M or less.
  • the term “subject” includes any human or nonhuman animal.
  • nonhuman animal includes all vertebrates, e.g., mammals and non-mammals, such as nonhuman primates, sheep, dogs, cats, horses, cows, chickens, amphibians, reptiles, etc.
  • alkyl by itself or as part of another substituent, means, unless otherwise stated, a straight or branched chain, or cyclic hydrocarbon radical, or combination thereof, which may be fully saturated, mono- or polyunsaturated and can include di- and multivalent radicals, having the number of carbon atoms designated (i.e., C
  • saturated hydrocarbon radicals include, but are not limited to, groups such as methyl, ethyl, n-propyl, isopropyl.
  • An unsaturated alkyl group is one having one or more double bonds or triple bonds.
  • alkyl groups examples include, but are not limited to, vinyl, 2-propenyl, crotyl, 2-isopentenyl, 2-(butadienyl), 2,4-pentadienyl, 3-(l,4-pentadienyl). ethynyl, 1- and 3-propynyl, 3-butynyl, and the higher homologs and isomers.
  • alkyl unless otherwise noted, is also meant to include those derivatives of alkyl defined in more detail below, such as “heteroalkyl.”
  • Alkyl groups, which are limited to hydrocarbon groups are termed "homoalkyl".
  • alkylene by itself or as part of another substituent means a divalent radical derived from an alkane, as exemplified, but not limited, by -CHiCH 2 CH 2 CH?-, and further includes those groups described below as “heteroalkylene.”
  • an alkyl (or alkylene) group will have from 1 to 24 carbon atoms, with those groups ha ⁇ ing 10 or fewer carbon atoms being preferred in the present invention.
  • a '"lower alkyl” or “lower alkylene” is a shorter chain alkyl or alkylene group, generally having eight or fewer carbon atoms.
  • heteroalkyl by itself or in combination with another term, means, unless otherwise stated, a stable straight or branched chain, or cyclic hydrocarbon radical, or combinations thereof, consisting of the stated number of carbon atoms and at least one heteroatom selected from the group consisting of O, N, Si, and S, and wherein the nitrogen, carbon and sulfur atoms may optionally be oxidized and the nitrogen heteroatom may optionally be quaternized.
  • the heteroatom(s) O, N, S, and Si may be placed at any interior position of the heteroalkyl group or at the position at which the alkyl group is attached to the remainder of the molecule.
  • heteroalkylene by itself or as part of another substituent means a divalent radical derived from heteroalkyl, as exemplified, but not limited by, -CH2-CH2-S-CH2-CH2- and --CH 2 -S-CH 2 -CH 2 -NH-CH 2 -.
  • heteroatoms can also occupy either or both of the chain termini (e.g., alkyleneoxy, alkylenedioxy, alkyleneamino, alkylenediamino, and the like).
  • heteroalkyl and “heteroalkylene” encompass poly(ethylene glycol) and its derivatives (see, for example, Shearwater Polymers Catalog, 2001). Still further, for alkylene and heteroalkylene linking groups, no orientation of the linking group is implied by the direction in which the formula of the linking group is written. For example, the formula -C(O) 2 R * - represents both -C(O) 2 R'- and -R 1 C(O) 2 -.
  • lower in combination with the terms “alkyl” or “heteroalkyl " refers to a moiety having from 1 to 6 carbon atoms.
  • alkylamino alkylsulfonyl
  • alkylthio or thioalkoxy
  • arylsulfonyl refers to an aryl group attached to the remainder of the molecule via an SO 2 group
  • sulfhydryl refers to an SH group.
  • an "acyl substituent" is also selected from the group set forth above.
  • acyl substituent refers to groups attached to, and fulfilling the valence of a carbonyl carbon that is either directly or indirectly attached to the polycyclic nucleus of the compounds of the present invention.
  • cycloalkyl and “heterocycloalkyl”, by themselves or in combination with other terms, represent, unless otherwise stated, cyclic versions of substituted or unsubstituted “alkyl” and substituted or unsubstituted “heteroalkyl”, respectively. Additionally, for heterocycloalkyl, a heteroatom can occupy the position at which the heterocycle is attached to the remainder of the molecule. Examples of cycloalkyl include, but are not limited to, cyclopentyl, cyclohexyl, 1-cyclohexenyl, 3-cyclohexenyl, cycloheptyl, and the like.
  • heterocycloalkyl examples include, but are not limited to, 1 -(1,2,5,6-tetrahydropyridyl), 1-piperidinyl, 2-piperidinyl, 3-piperidinyl, 4-morpholinyl, 3-morpholinyl, tetrahydrofuran-2-yl, tetrahydrofuran-3-yl, tetrahydrothien-2-yl, tetrahydrothien-3-yl, 1 -piperazinyl, 2-piperazinyl, and the like.
  • the heteroatoms and carbon atoms of the cyclic structures are optionally oxidized.
  • halo or halogen
  • haloalkyl by themselves or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.
  • terms such as “haloalkyl,” are meant to include monohaloalkyl and polyhaloalkyl.
  • 'halo(Ci-C 4 )alkyr is meant to include, but not be limited to, trifluoromethyl, 2,2,2-trifluor ⁇ ethyl, 4-chlorobutyl, 3-bromopropyl, and the like.
  • aryl means, unless otherwise stated, a substituted or unsubstituted polyunsaturated, aromatic, hydrocarbon substituent which can be a single ring or multiple rings (preferably from 1 to 3 rings) which are fused together or linked covalently.
  • heteroaryl refers to aryl groups (or rings) that contain from one to four heteroatoms selected from N, O, and S, wherein the nitrogen, carbon and sulfur atoms are optionally oxidized, and the nitrogen atom(s) are optionally quatemized.
  • a heteroaryl group can be attached to the remainder of the molecule through a heteroatom.
  • Non- limiting examples of aryl and heteroaryl groups include phenyl, 1-naphthyl, 2-naphthyl, 4-biphenyl, 1-pyrrolyl, 2-pyrrolyl, 3-pyrrolyl, 3-pyrazolyl, 2-imidazolyl, 4-imidazolyl, pyrazinyl, 2-oxazolyl, 4-oxazolyl, 2-phenyl-4-oxazolyl, 5-oxazolyl, 3-isoxazolyl, A- isoxazolyl, 5-isoxazolyl, 2-thiazolyl, 4-thiazolyl, 5-thiazolyl, 2-furyl, 3-furyl, 2-thienyl, 3-thienyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, 2-pyrimidyl, 4-pyrimidyl, 5-benzothiazolyl, purinyl, 2-benzimidazolyl, 5-indolyl, 1-iso
  • aryl and heteroaryl ring systems are selected from the group of acceptable substituents described below.
  • Aryl and “heteroaryl” also encompass ring systems in which one or more non-aromatic ring systems are fused, or otherwise bound, to an aryl or heteroaryl system.
  • aryF when used in combination with other terms (e.g., aryloxy, arylthioxy, arylalkyl) includes both aryl and heteroaryl rings as defined above.
  • arylalkyP is meant to include those radicals in which an aryl group is attached to an alkyl group (e.g., benzyl, phenethyl, pyridylmethyl and the like) including those alkyl groups in which a carbon atom (e.g., a methylene group) has been replaced by, for example, an oxygen atom (e.g., phenoxymethyl, 2-pyridyloxymethyl, 3-(l- naphthyloxy)propyl, and the like).
  • alkyl group e.g., benzyl, phenethyl, pyridylmethyl and the like
  • oxygen atom e.g., phenoxymethyl, 2-pyridyloxymethyl, 3-(l- naphthyloxy)
  • R', R", R"' * and R" each preferably independently refer to hydrogen, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, e.g., aryl substituted with 1-3 halogens, substituted or unsubstituted alkyl, alkoxy or thioalkoxy groups, or arylalkyl groups.
  • each of the R groups is independently selected as are each R ⁇ R", R 1 " and R'"' groups when more than one of these groups is present.
  • R' and R" are attached to the same nitrogen atom, they can be combined with the nitrogen atom to form a 5, 6, or 7-membered ring.
  • -NR'R is meant to include, but not be limited to, l ⁇ pyrrolidinyl and 4- morpholinyl.
  • alkyl is meant to include groups including carbon atoms bound to groups other than hydrogen groups, such as haloalkyl (e.g., -CF 3 and -CH 2 CF 3 ) and acyl (e.g., -C(O)CH 3 , -C(O)CF 3 , -C(O)CH 2 OCH 3 , and the like).
  • -NR-C(NR " R") NR'",.-S(O)R ⁇ -S(O) 2 R 5 , -S(O) 2 NR 5 R", -NRSO 2 R', -CN and -NO 2 , - R', -Nj, -CH(Ph)O, fluoro(C r C 4 )alkoxy, and fluoro(C
  • R group for example
  • Two of the aryl substituents on adjacent atoms of the aryl or heteroaryl ring may optionally be replaced with a substituent of the formula -T-C(O)-(CRR' ) q - U-, wherein T and U are independently -NR-, -O-, -CRR'- or a single bond, and q is an integer of from 0 to 3.
  • two of the substituents on adjacent atoms of the aryl or heteroaryl ring may optionally be replaced with a substituent of the formula -A-(CHo) 1 -B-, wherein A and B are independently -CRR'-, -O-, -NR-, -S-, -S(O)-, -S(O) 2 -, -S(O) 2 NR 1 - or a single bond, and r is an integer of from 1 to 4.
  • One of the single bonds of the new ring so formed may optionally be replaced with a double bond.
  • two of the substituents on adjacent atoms of the aryl or heteroaryl ring may optionally be replaced with a substituent of the formula -(CRR') s -X-(CR"R “ ")d-, where s and d are independently integers of from 0 to 3, and X is -O-, -NR'-, -S-, -S(O)-, -S(O) 2 -, or -- S(O) 2 NR'-.
  • the substituents R, R', R" and R"" are preferably independently selected from hydrogen or substituted or unsubstituted (Ci-C 6 ) alkyl.
  • diphosphate includes but is not limited to an ester of phosphoric acid containing two phosphate groups.
  • triphosphate includes but is not limited to an ester of phosphoric acid containing three phosphate groups.
  • drugs having a diphosphate or a triphosphate include:
  • heteroatom includes oxygen (O), nitrogen (N), sulfur (S) and silicon (Si).
  • R is a general abbreviation that represents a substituent group that is selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocyclyl groups.
  • the antibodies of this disclosure are characterized by particular functional features or properties of the antibodies.
  • the antibodies specifically bind to human CD 19.
  • an antibody of this disclosure binds to CD 19 with high affinity, for example with a K D of 1 x 10 " M or less.
  • the anti-CD 19 antibodies of this disclosure preferably exhibit one or more of the following characteristics:
  • the antibody exhibits at least two of properties (a), (b), (c), (d), and (e). More preferably, the antibody exhibits at least three of properties (a), (b), (c), (d), and (e). More preferably, the antibody exhibits four of properties (a), (b), (c), (d), and (e). Even, more preferably, the antibody exhibits all five of properties (a), (b), (c), (d), and (e). In another preferred embodiment, the antibody inhibits growth of CD 19- expressing tumor cells in vivo when the antibody is conjugated to a cytotoxin.
  • the antibody binds to human CD 19 with a K D of 5 x 10 s M or less, binds to human CD 19 with a K 0 of 1 x 10 "8 M or less, binds to human CD 19 with a K D of 5 x 10 "9 M or less, binds to human CD 19 with a K D of 4 X 10 "9 M or less, binds to human CD 19 with a K D of 3 x 10 "9 M or less, or binds to human CD 19 with a Kp of 2 x 10 "9 M or less, or binds to human CD 19 with a K D of 1 x l ⁇ " ⁇ M or less.
  • an antibody of the invention can be assessed using one or more techniques well established in the art.
  • an antibody can be tested by a flow cytometry assay in which the antibody is reacted with a cell line that expresses human CD 19, such as CHO cells that have been transfected to express CD 19 on their cell surface or CD19-expressing cell lines such as OVCAR3, NCI- H226, CFPAC-I and/or KB (see, e.g., Example 3 A for a suitable assay and further description of cell lines).
  • the binding of the antibody can be tested in BIAcore binding assays (see , e.g., Example 3B for suitable assays).
  • BIAcore binding assays see , e.g., Example 3B for suitable assays.
  • Still other suitable binding assays include ELISA assays, for example using a recombinant CD 19 protein see, e.g., Example 1 for a suitable assay).
  • an antibody of this disclosure binds to a CD 19 protein with a KD of 5 x 10-8 M or less, binds to a CD 19 protein with a KD of 3 x 10-8 M or less, binds to a
  • CD 19 protein with a KD of 1 x 10-8 M or less binds to a CD 19 protein with a KD of 7 x 10-9 M or less, binds to a CD 19 protein with a KD of 6 x 10-9 M or less or binds to a CD 19 protein with a KD of 5 x 10-9 M or less.
  • the binding affinity of the antibody for CD 19 can be evaluated, for example, by standard BIACORE analysis, (see e.g., Example 3B).
  • Standard assays for evaluating internalization of anti-CD 19 antibodies by CD 19- expressing cells are known in the art (see e.g., the Hum-ZAP and immunofluorescence assays described in Example 5).
  • Standard assays for evaluating binding of CD 19 to CAl 25, and inhibition thereof by anti-CD 19 antibodies also are known in the art (see e.g., the OVCAR3 cell adhesion assay described in Example 6).
  • Standard assays for evaluating ADCC against CD19-expressing cells also are known in the art (see e.g., the ADCC assay described in Example 7).
  • Preferred antibodies of the invention are human monoclonal antibodies. Additionally or alternatively, the antibodies can be, for example, chimeric or humanized monoclonal antibodies.
  • Preferred antibodies of this disclosure are the human monoclonal antibodies 21D4, 2lD4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8, isolated and structurally characterized as described in Examples 16, 17, 18, 19, 20, 21 and 22.
  • the Vn amino acid sequences of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 1, 1, 2, 3, 4, 5, 6 and 7, respectively.
  • 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 8, 9, 10, 1 1, 12, 13, 14 and 15, respectively.
  • sequences can be "mixed and matched" to create other anti-CD 19 binding molecules of this disclosure.
  • CD 19 binding of such "mixed and matched” antibodies can be tested using the binding assays described above and in the Examples (e.g., ELISAs).
  • a V H sequence from a particular Vn/V ⁇ pairing is replaced with a structurally similar V H sequence.
  • a V 1 sequence from a particular V 5 /V L pairing is replaced with a structurally similar V L sequence.
  • this disclosure provides an isolated monoclonal antibody, or antigen binding portion thereof comprising:
  • a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6 and 7; and (b) a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 9, 10, 1 1, 12, 13, 14 and 15; wherein the antibody specifically binds CD 19, preferably human CD 19.
  • Preferred heavy and light chain combinations include:
  • this disclosure provides antibodies that comprise the heavy chain and light chain CDRIs, CDR2s and CDR3s of 21D4, 21D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8, or combinations thereof.
  • the amino acid sequences of the V H CDRIs of 21D4, 21D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22.
  • the amino acid sequences of the V H CDR2s of 21D4, 21D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29.
  • amino acid sequences of the Vn CDR3s of 21D4, 21D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36.
  • amino acid sequences of the V k CDRIs of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43.
  • the amino acid sequences of the V k CDR2s of 21 D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50.
  • the amino acid sequences of the V k CDR3s of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58.
  • the CDR regions are delineated using the Kabat system (Kabat, E. A., et ai (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242).
  • Vn CDRl, CDR2, and CDR3 sequences and V k CDRl, CDR2, and CDR3 sequences can be "mixed and matched" ⁇ i.e., CDRs from different antibodies can be mixed and match, although each antibody must contain a V H CDRl, CDR2, and CDR3 and a V k CDRl, CDR2, and CDR3) to create other anti-CD 19 binding molecules of this disclosure.
  • CD 19 binding of such "mixed and matched" antibodies can be tested using the binding assays described above and in the Examples ⁇ e.g., ELISAs, Biacore* analysis).
  • V H CDR sequences are mixed and matched
  • the CDR 1 , CDR2 and/or CDR3 sequence from a particular V H sequence is replaced with a structurally similar CDR sequence(s).
  • V k CDR sequences are mixed and matched
  • the CDRl, CDR2 and/or CDR3 sequence from a particular V ⁇ sequence preferably is replaced with a structurally similar CDR sequence(s).
  • this disclosure provides an isolated monoclonal antibody, or antigen binding portion thereof comprising:
  • a heavy chain variable region CDRl comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22;
  • a heavy chain variable region CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29
  • a heavy chain variable region CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36;
  • a light chain variable region CDRl comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43;
  • a light chain variable region CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50;
  • a light chain variable region CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58; wherein the antibody specifically binds CD 19, preferably human CD 19.
  • the antibody comprises:
  • the antibody comprises:
  • the antibody comprises: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 17;
  • the antibody comprises: (a) a heavy chain variable region CDR 1 comprising SEQ ID NO: 18;
  • the antibody comprises:
  • the antibody comprises: (a) a heavy chain variable region CDR 1 comprising SEQ ID NO: 20;
  • the antibody comprises:
  • the antibody comprises:
  • the CDR3 domain independently from the CDRl and/or CDR2 domain(s), alone can determine the binding specificity of an antibody for a cognate antigen and that multiple antibodies can predictably be generated having the same binding specificity based on a common CDR3 sequence. See, for example, Klimka et al., British J. of Cancer 83(2):252-260 (2000) (describing the production of a humanized anti-CD30 antibody using only the heavy chain variable domain CDR3 of murine anti-CD30 antibody Ki-4); Beiboer et al, J. MoL Biol.
  • Biochem (Tokyo) 117:452-7 (1995) (describing a 12 amino acid synthetic polypeptide corresponding to the CDR3 domain of an anti-phosphatidylserine antibody); Bourgeois et al, J. Virol 72:807- 10 (1998) (showing that a single peptide derived from the heavy chain CDR3 domain of an anti-respiratory syncytial virus (RSV) antibody was capable of neutralizing the virus in vitro); Levi et uL, Proc. Natl. Acad. Sci. U.S.A. 90:4374-8 (1993) (describing a peptide based on the heavy chain CDR3 domain of a murine anti-HIV antibody); Polymenis and Stoller, J.
  • RSV anti-respiratory syncytial virus
  • the present disclosure provides monoclonal antibodies comprising one or more heavy and/or light chain CDR3 domains from an antibody derived from a human or non-human animal, wherein the monoclonal antibody is capable of specifically binding to CD 19.
  • the present disclosure provides monoclonal antibodies comprising one or more heavy and/or light chain CDR3 domain from a non- human antibody, such as a mouse or rat antibody, wherein the monoclonal antibody is capable of specifically binding to CD 19.
  • inventive antibodies comprising one or more heavy and/or light chain CDR3 domain from a non- human antibody (a) are capable of competing for binding with; (b) retain the functional characteristics; (c) bind to the same epitope; and/or (d) have a similar binding affinity as the corresponding parental non-human antibody.
  • the present disclosure provides monoclonal antibodies comprising one or more heavy and/or light chain CDR3 domain from a human antibody, such as, for example, a human antibody obtained from a non-human animal, wherein the human antibody is capable of specifically binding to CD 19.
  • a human antibody such as, for example, a human antibody obtained from a non-human animal
  • the present disclosure provides monoclonal antibodies comprising one or more heavy and/or light chain CDR3 domain from a first human antibody, such as, for example, a human antibody obtained from a non-human animal, wherein the first human antibody is capable of specifically binding to CD 19 and wherein the CDR3 domain from the first human antibody replaces a CDR3 domain in a human antibody that is lacking binding specificity for CD 19 to generate a second human antibody that is capable of specifically binding to CD 19.
  • inventive antibodies comprising one or more heavy and/or light chain CDR3 domain from the first human antibody (a) are capable of competing for binding with; (b) retain the functional characteristics; (c) bind to the same epitope: and/or (d) have a similar binding affinity as the corresponding parental first human antibody.
  • an antibody of this disclosure comprises a heavy chain variable region from a particular germline heavy chain immunoglobulin gene and/or a light chain variable region from a particular germline light chain immunoglobulin gene.
  • this disclosure provides an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a heavy chain variable region that is the product of or derived from a human V H 5-51 gene, wherein the antibody specifically binds CD 19.
  • this disclosure provides an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a heavy chain variable region that is the product of or derived from a human V H 1-69 gene, wherein the antibody specifically binds CD 19.
  • this disclosure provides an isolated monoclonal antibody, or an antigen- binding portion thereof, comprising a light chain variable region that is the product of or derived from a human V K Ll 8 gene, wherein the antibody specifically binds CD 19.
  • this disclosure provides an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a light chain variable region that is the product of or derived from a human V K A27 gene, wherein the antibody specifically binds CD 19.
  • this disclosure provides an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a light chain variable region that is the product of or derived from a human V K Ll 5 gene, wherein the antibody specifically binds CD 19.
  • this disclosure provides an isolated monoclonal antibody, or antigen-binding portion thereof, wherein the antibody: (a) comprises a heavy chain variable region that is the product of or derived from a human Vn 5-51 or 1-69 gene (which genes encode the amino acid sequences set forth in SEQ ID NOs: 74 and 75, respectively);
  • (b) comprises a light chain variable region that is the product of or derived from a human V K L 18, V ⁇ A27 or V ⁇ Ll 5 gene (which genes encode the amino acid sequences set forth in SEQ ID NOs: 76, 77 and 78, respectively); and
  • (c) specifically binds to CD 19, preferably human CD 19.
  • Such antibodies also may possess one or more of the functional characteristics described in detail above, such as high affinity binding to human CD 19, internalization by CD 19-expressing cells, the ability to mediate ADCC against CD 19-expressing cells and/or the ability to inhibit tumor growth of CDl 9-expressing tumor cells in vivo when conjugated to a cytotoxin.
  • Examples of antibodies having V H and V ⁇ of V H 5-51 and V K L18, respectively. are 21D4, 21D4a, 27F3, 5G7, 13Fl and 46E8.
  • An example of an antibody having V H and V ⁇ of V H 1-69 and V K A27, respectively, is 47G4.
  • An example of an antibody having Vn and V ⁇ of Vn 1-69 and V ⁇ L15, respectively, is 3C10.
  • a human antibody comprises heavy or light chain variable regions that is "the product of or '"derived from” a particular germline sequence if the variable regions of the antibody are obtained from a system that uses human germline immunoglobulin genes.
  • Such systems include immunizing a transgenic mouse carrying human immunoglobulin genes with the antigen of interest or screening a human immunoglobulin gene library displayed on phage with the antigen of interest.
  • a human antibody that is "the product of” or “derived from” a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequences of human germline immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence (i.e., greatest % identity) to the sequence of the human antibody.
  • a human antibody that is "the product of or "derived from” a particular human germline immunoglobulin sequence may contain amino acid differences as compared to the gennline sequence, due to, for example, naturally-occurring somatic mutations or intentional introduction of site- directed mutation.
  • a selected human antibody typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline immunoglobulin gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g., murine germline sequences).
  • a human antibody may be at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene.
  • a human antibody derived from a particular human germline sequence will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene.
  • the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid sequence encoded by the germline immunoglobulin gene.
  • an antibody of this disclosure comprises heavy and light chain variable regions comprising amino acid sequences that are homologous to the amino acid sequences of the preferred antibodies described herein, and wherein the antibodies retain the desired functional properties of the anti-CD 19 antibodies of this disclosure.
  • this disclosure provides an isolated monoclonal antibody, or antigen binding portion thereof, comprising a heavy chain variable region and a light chain variable region, wherein:
  • the heavy chain variable region comprises an amino acid sequence that is at least 80% homologous to an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6 and 7;
  • the light chain variable region comprises an amino acid sequence that is at least 80% homologous to an amino acid sequence selected from the group consisting of
  • the antibody may possess one or more of the following functional properties discussed above, such as high affinity binding to human CD 19, internalization by CD19-expressing cells, the ability to mediate ADCC against CD 19-expressing cells and/or the ability to inhibit tumor growth of CD 19-expressing tumor cells in vivo when conjugated to a cytotoxin.
  • the antibody can be, for example, a human antibody, a humanized antibody or a chimeric antibody.
  • the V H and/or V L amino acid sequences may be 85%, 90%,
  • An antibody having V H and V L regions having high (i.e., 80% or greater) homology to the Vn and V L regions of the sequences set forth above can be obtained by mutagenesis (e.g., site- directed or PCR-mediated mutagenesis) of nucleic acid molecules encoding SEQ ID NOs: 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72 or 73, followed by testing of the encoded altered antibody for retained function (i.e., the functions set forth in (c) through (d) above) using the functional assays described herein.
  • mutagenesis e.g., site- directed or PCR-mediated mutagenesis
  • the percent homology between two amino acid sequences is equivalent to the percent identity between the two sequences.
  • the comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm, as described in the non-limiting examples below.
  • the percent identity between two amino acid sequences can be determined using the algorithm of E. Meyers and W. Miller (Comput. Appl. Biosci., 4: 1 1-17 (1988)) which has been incorporated into the ALIGN program (version 2.0), using a PAM 120 weight residue table, a gap length penalty of 12 and a gap penalty of 4.
  • the percent identity between two amino acid sequences can be determined using the Needleman and Wunsch (J. MoI. Biol.
  • the protein sequences of the present disclosure can further be used as a "query sequence" to perform a search against public databases to, for example, identify related sequences. Such searches can be performed using the XBLAST program (version 2.0) of Altschul, et al. (1990) J. MoI. Biol. 215:403-10.
  • Gapped BLAST can be utilized as described in Altschul et al., (1997) Nucleic Acids Res. 25(17):3389-3402.
  • the default parameters of the respective programs e.g., XBLAST and NBLAST are useful. See www.ncbi.nlm.nih.gov.
  • an antibody of this disclosure comprises a heavy chain variable region comprising CDRl, CDR2 and CDR3 sequences and a light chain variable region comprising CDRl , CDR2 and CDR3 sequences, wherein one or more of these CDR sequences comprise specified amino acid sequences based on known anti-CD 19 antibodies, or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the anti-CD 19 antibodies of this disclosure. It is understood in the art that certain conservative sequence modification can be made which do not remove antigen binding. See, for example, Brummell et al. (1993) Biochem 32:1180-8 (describing mutational analysis in the CDR3 heavy chain domain of antibodies specific for Salmonella); de Wildt et al.
  • this disclosure provides an isolated monoclonal antibody, or antigen binding portion thereof, comprising a heavy chain variable region comprising CDRl, CDR2, and CDR3 sequences and a light chain variable region comprising CDRl, CDR2, and CDR3 sequences, wherein: (a) the heavy chain variable region CDR3 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36, and conservative modifications thereof;
  • the light chain variable region CDR3 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequence of SEQ ID NOs: 51 , 52, 53, 54, 55, 56, 57 and 58, and conservative modifications thereof;
  • the antibody may possess one or more of the following functional properties described above, such as high affinity binding to human CD 19, internalization by CD19-expressing cells, the ability to mediate ADCC against CD19-expressing cells and/or the ability to inhibit tumor growth of CD19-expressing tumor cells in vivo when conjugated to a cytotoxin.
  • the heavy chain variable region CDR2 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, and conservative modifications thereof; and the light chain variable region CDR2 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50, and conservative modifications thereof.
  • the heavy chain variable region CDRl sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22, and conservative modifications thereof; and the light chain variable region CDRl sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43, and conservative modifications thereof.
  • the antibody can be, for example, human antibodies, humanized antibodies or chimeric antibodies.
  • conservative sequence modifications are intended to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence. Such conservative modifications include amino acid substitutions, additions and deletions. Modifications can be introduced into an antibody of this disclosure by standard techniques known in the art, such as site-directed mutagenesis and PCR-mediated mutagenesis. Conservative amino acid substitutions are ones in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art.
  • amino acids with basic side chains e.g., lysine, arginine, histidine
  • acidic side chains e.g., aspartic acid, glutamic acid
  • uncharged polar side chains e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine, tryptophan
  • nonpolar side chains e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine
  • beta-branched side chains e.g., threonine, valine, isoleucine
  • aromatic side chains e.g., tyrosine, phenylalanine, tryptophan, histidine
  • one or more amino acid residues within the CDR regions of an antibody of this disclosure can be replaced with other amino acid residues from the same side chain family and the altered antibody can be tested for retained function (/ e , the functions set forth in (c) through (d) above) using the functional assays described herein.
  • this disclosure provides antibodies that bind an epitope on human CD 19 recognized by any of the CD 19 monoclonal antibodies of this disclosure (i.e., antibodies that have the ability to cross-compete for binding to CD 19 with any of the monoclonal antibodies of this disclosure).
  • the reference antibody for cross-competition studies can be the monoclonal antibody 21D4 (having V H and V L sequences as shown in SEQ ID NOs: 1 and 8, respectively), or the monoclonal antibody 21D4a (having V H and V L sequences as shown in SEQ ID NOs: 1 and 9, respectively), or the monoclonal antibody 47G4 (having V H and V L sequences as shown in SEQ ID NOs: 2 and 10, respectively), or the monoclonal antibody 27F3 (having Vn and V L sequences as shown in SEQ ID NOs: 3 and 1 1, respectively), or the monoclonal antibody 3C10 (having V H and V L sequences as shown in SEQ ID NOs: 4 and 12, respectively), or the monoclonal antibody 5G7 (having V H and V L sequences as shown in SEQ ID NOs: 5 and 13, respectively), or the monoclonal antibody 13Fl (having V H and V L sequences as shown in SEQ ID NOs: 6 and 14, respectively
  • cross-competing antibodies can be identified based on their ability to cross-compete with 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 in standard CD 19 binding assays.
  • Standard ELISA assays can be used in which a recombinant human CD 19 protein is immobilized on the plate, one of the antibodies is fluorescently labeled and the ability of non-labeled antibodies to compete off the binding of the labeled antibody is evaluated.
  • BIAcore analysis can be used to assess the ability of the antibodies to cross-compete.
  • test antibody to inhibit the binding of, for example, 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8, to human CD 19 demonstrates that the test antibody can compete with 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 for binding to human CD19 and thus binds to the same epitope on human CD 19 as 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8.
  • the antibody that binds to the same epitope on human CD 19 as 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 is a human monoclonal antibody.
  • human monoclonal antibodies can be prepared and isolated as described in the Examples.
  • An antibody of the invention can further be prepared using an antibody having one or more known CDl 9 antibody V H and/or V 1 sequences can be used as starting material to engineer a modified antibody, which modified antibody may have altered properties as compared to the starting antibody.
  • An antibody can be engineered by modifying one or more amino acids within one or both variable regions (i.e., V H and/or V L ), for example, within one or more CDR regions and/or within one or more framework regions. Additionally or alternatively, an antibody can be engineered by modifying residues within the constant region(s), for example, to alter the effector function(s) of the antibody.
  • CDR grafting can be used to engineer variable regions of antibodies.
  • Antibodies interact with target antigens predominantly through amino acid residues that are located in the six heavy and light chain complementarity determining regions (CDRs). For this reason, the amino acid sequences within CDRs are more diverse between individual antibodies than sequences outside of CDRs. Because CDR sequences are responsible for most antibody-antigen interactions, it is possible to express recombinant antibodies that mimic the properties of specific naturally occurring antibodies by constructing expression vectors that include CDR sequences from the specific naturally occurring antibody grafted onto framework sequences from a different antibody with different properties (see, e.g., Riechmann, L. et al. (1998) Nature 332:323- 327; Jones, P.
  • an isolated monoclonal antibody, or antigen binding portion thereof comprising a heavy chain variable region comprising CDRl, CDR2, and CDR3 sequences comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22, SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, and SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36, respectively, and a light chain variable region comprising CDRl, CDR2, and CDR3 sequences comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43, SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50, and SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58, respectively.
  • such antibodies contain the V H and V L CDR
  • Such framework sequences can be obtained from public DNA databases or published references that include germline antibody gene sequences.
  • germline DNA sequences for human heavy and light chain variable region genes can be found in the "VBase" human germline sequence database (available on the Internet at www.mrc-cpe.cam.ac.uk/vbase), as well as in Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242; Tomlinson, I. M., et al.
  • NG_0010109, NTJ)24637 and BC070333 3-33 (NG_0010109 and NTJ)24637) and 3- 7 (NGJ3O1O1O9 and NT_024637).
  • the following heavy chain germline sequences found in the HCo 12 HuMAb mouse are available in the accompanying Genbank accession numbers: 1-69 (NGJ)OlO 109, NT_024637 and BC070333), 5-51 (NGJ)OlO 109 and NTJ)24637), 4-34 (NGJ)010109 and NT_024637), 3-30.3 (CAJ556644) and 3-23 (A.T406678).
  • Yet another source of human heavy and light chain germline sequences is the database of human immunoglobulin genes available from IMGT (http://imgt.cines.fr).
  • Antibody protein sequences are compared against a compiled protein sequence database using one of the sequence similarity searching methods called the Gapped BLAST (Altschul et al. (1997) Nucleic Acids Research 25:3389-3402), which is well known to those skilled in the art.
  • BLAST is a heuristic algorithm in that a statistically significant alignment between the antibody sequence and the database sequence is likely to contain high-scoring segment pairs (HSP) of aligned words. Segment pairs whose scores cannot be improved by extension or trimming is called a hit.
  • HSP high-scoring segment pairs
  • nucleotide sequences of VBASE origin ⁇ http://vbase.mrc-cpe.cam.ac.uk/vbase 1 /Iist2.php
  • the database sequences have an average length of 98 residues.
  • Duplicate sequences which are exact matches over the entire length of the protein are removed.
  • the nucleotide sequences are translated in all six frames and the frame with no stop codons in the matching segment of the database sequence is considered the potential hit.
  • BLAST program tblastx which translates the antibody sequence in all six frames and compares those translations to the VBASE nucleotide sequences dynamically translated in all six frames.
  • Other human germline sequence databases such as that available from IMGT (http://imgt.cines.fr), can be searched similarly to VBASE as described above.
  • the identities are exact amino acid matches between the antibody sequence and the protein database over the entire length of the sequence.
  • the positives are not identical but amino acid substitutions guided by the BLOSUM62 substitution matrix. If the antibody sequence matches two of the database sequences with same identity, the hit with most positives would be decided to be the matching sequence hit.
  • Preferred framework sequences for use in the antibodies of this disclosure are those that are structurally similar to the framework sequences used by selected antibodies of this disclosure, e.g., similar to the Vn 5-51 framework sequences (SEQ ID NO: 74) and/or the V H 1-69 framework sequences (SEQ ID NO: 75) and/or the V K L 18 framework sequences (SEQ ID NO: 76) and/or the V ⁇ A27 framework sequence (SEQ ID NO: 77) and/or the V ⁇ Ll 5 framework sequence (SEQ ID NO: 78) used by preferred monoclonal antibodies of this disclosure.
  • V H CDRl, CDR2, and CDR3 sequences, and the V K CDRl, CDR2, and CDR3 sequences can be grafted onto framework regions that have the identical sequence as that found in the germline immunoglobulin gene from which the framework sequence derive, or the CDR sequences can be grafted onto framework regions that contain one or more mutations as compared to the germline sequences.
  • variable region modification is to mutate amino acid residues within the V H and/or V K CDRl, CDR2 and/or CDR3 regions to thereby improve one or more binding properties ⁇ e.g., affinity) of the antibody of interest.
  • Site-directed mutagenesis or PCR-mediated mutagenesis can be performed to introduce the mutation(s) and the effect on antibody binding, or other functional property of interest, can be evaluated in in vitro or in vivo assays as described herein and provided in the Examples.
  • Preferably conservative modifications are introduced.
  • the mutations may be amino acid substitutions, additions or deletions, but are preferably substitutions.
  • typically no more than one, two, three, four or five residues within a CDR region are altered.
  • the instant disclosure provides isolated anti- CD 19 monoclonal antibodies, or antigen binding portions thereof, comprising a heavy chain variable region comprising: (a) a Vu CDRl region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22; (b) a Vn CDR2 region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29; (c) a V H CDR3 region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36, or an amino acid sequence having one, two
  • Engineered antibodies of this disclosure include those in which modifications have been made to framework residues within V H and/or V «, e.g. to improve the properties of the antibody. Typically such framework modifications are made to decrease the immunogenicity of the antibody. For example, one approach is to "backmutate' ' one or more framework residues to the corresponding germline sequence. More specifically, an antibody that has undergone somatic mutation may contain framework residues that differ from the germline sequence from which the antibody is derived. Such residues can be identified by comparing the antibody framework sequences to the germline sequences from which the antibody is derived.
  • Table 1 shows a number of amino acid changes in the framework regions of the anti-PD-1 antibodies 17D8, 2D3, 4Hl, 5C4, 4Al 1, 7D3 and 5F4 that differ from the heavy chain parent germline sequence.
  • somatic mutations can be "backmutated" to the germline sequence by, for example, site-directed mutagenesis or PCR-mediated mutagenesis.
  • Ant ⁇ -CD19 Ab Amino acid position Amino acid of Original amino acid of germline antibody configuration
  • Another type of framework modification involves mutating one or more residues within the framework region, or even within one or more CDR regions, to remove T cell epitopes to thereby reduce the potential immunogenicity of the antibody. This approach is also referred to as "deimmunization" and is described in further detail in U.S. Patent Publication No. 20030153043 by Can- et al.
  • antibodies of this disclosure may be engineered to include modifications within the Fc region, typically to alter one or more functional properties of the antibody, such as serum half-life, complement fixation, Fc receptor binding, and/or antigen-dependent cellular cytotoxicity.
  • an antibody of this disclosure may be chemically modified (e.g., one or more chemical moieties can be attached to the antibody) or be modified to alter its glycosylation, again to alter one or more functional properties of the antibody.
  • chemically modified e.g., one or more chemical moieties can be attached to the antibody
  • modify its glycosylation again to alter one or more functional properties of the antibody.
  • the hinge region of CHl is modified such that the number of cysteine residues in the hinge region is altered, e.g., increased or decreased.
  • This approach is described further in U.S. Patent No. 5,677,425 by Bodmer et al.
  • the number of cysteine residues in the hinge region of CHl is altered to, for example, facilitate assembly of the light and heavy chains or to increase or decrease the stability of the antibody.
  • the Fc hinge region of an antibody is mutated to decrease the biological half life of the antibody. More specifically, one or more amino acid mutations are introduced into the CH2-CH3 domain interface region of the Fc -hinge fragment such that the antibody has impaired Staphylococcyl protein A (SpA) binding relative to native Fc-hinge domain SpA binding.
  • SpA Staphylococcyl protein A
  • the antibody is modified to increase its biological half life.
  • the antibody is modified to increase its biological half life.
  • Various approaches are possible. For example, one or more of the following mutations can be introduced: T252L, T254S, T256F, as described in U.S. Patent No.
  • the antibody can be altered within the CHl or C t . region to contain a salvage receptor binding epitope taken from two loops of a CH2 domain of an Fc region of an IgG, as described in U.S. Patent Nos. 5,869,046 and 6, 121,022 by Presta et al.
  • the Fc region is altered by replacing at least one amino acid residue with a different amino acid residue to alter the effector function(s) of the antibody.
  • one or more amino acids selected from amino acid residues 234, 235, 236, 237, 297, 318, 320 and 322 can be replaced with a different amino acid residue such that the antibody has an altered affinity for an effector ligand but retains the antigen- binding ability of the parent antibody.
  • the effector ligand to which affinity is altered can be, for example, an Fc receptor or the Cl component of complement. This approach is described in further detail in U.S. Patent Nos. 5,624,821 and 5,648,260, both by Winter et al.
  • one or more amino acids selected from amino acid residues 329, 331 and 322 can be replaced with a different amino acid residue such that the antibody has altered CIq binding and/or reduced or abolished complement dependent cytotoxicity (CDC).
  • CDC complement dependent cytotoxicity
  • one or more amino acid residues within amino acid positions 2316, 17, 18, 19, 20, 21 and 2239 are altered to thereby alter the ability of the antibody to fix complement. This approach is described further in PCT Publication WO 94/29351 by Bodmer et al.
  • the Fc region is modified to increase the ability of the antibody to mediate antibody dependent cellular cytotoxicity (ADCC) and/or to increase the affinity of the antibody for an Fc ⁇ receptor by modifying one or more amino acids at the following positions: 238, 239. 248, 249, 252, 254, 255, 256, 258, 265, 267, 268, 269, 270, 272, 276, 278, 280, 283, 285, 286, 289, 290, 292, 293, 294, 295, 296, 298, 301, 303, 305, 307, 309, 312, 315, 320, 322, 324, 326, 327, 329, 330.
  • ADCC antibody dependent cellular cytotoxicity
  • the C-terminal end of an antibody of the present invention is modified by the introduction of a cysteine residue as is described in U.S. Provisional Application Serial No. 60/957,271, which is hereby incorporated by reference in its entirety.
  • Such modifications include, but are not limited to, the replacement of an existing amino acid residue at or near the C-terminus of a full-length heavy chain sequence, as well as the introduction of a cysteine-containing extension to the c-terminus of a full-length heavy chain sequence.
  • the cysteine-containing extension comprises the sequence alanine-alanine-cysteine (from N-terminal to C- terminal).
  • the presence of such C-te ⁇ ninal cysteine modifications provide a location for conjugation of a partner molecule, such as a therapeutic agent or a marker molecule.
  • a partner molecule such as a therapeutic agent or a marker molecule.
  • the presence of a reactive thiol group, due to the C- terminal cysteine modification can be used to conjugate a partner molecule employing the disulfide linkers described in detail below. Conjugation of the antibody to a partner molecule in this manner allows for increased control over the specific site of attachment.
  • conjugation can be optimized such that it reduces or eliminates interference with the antibody's functional properties, and allows for simplified analysis and quality control of conjugate preparations.
  • the glycosylation of an antibody is modified.
  • an aglycoslated antibody can be made (i.e., the antibody lacks glycosylation).
  • Glycosylation can be altered to, for example, increase the affinity of the antibody for antigen.
  • carbohydrate modifications can be accomplished by, for example, altering one or more sites of glycosylation within the antibody sequence.
  • one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to thereby eliminate glycosylation at that site.
  • Such aglycosylation may increase the affinity of the antibody for antigen.
  • an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GlcNac structures.
  • altered glycosylation patterns have been demonstrated to increase the ADCC ability of antibodies.
  • carbohydrate modifications can be accomplished by, for example, expressing the antibody in a host cell with altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which to express recombinant antibodies of this disclosure to thereby produce an antibody with altered glycosylation.
  • the cell lines Ms704, Ms705, and Ms709 lack the fucosyltransferase gene, FUT8 (alpha ( 1 ,6) fucosyltransferase), such that antibodies expressed in the Ms704, Ms705, and Ms709 cell lines lack fucose on their carbohydrates.
  • the Ms704, Ms705, and Ms709 FUT8 ⁇ ; ⁇ cell lines were created by the targeted disruption of the FUT8 gene in CHO/DG44 cells using two replacement vectors (see U.S. Patent Publication No. 20040110704 by Yamane et al. and Yamane-Ohnuki et al. (2004) Biotechnol Bioeng 87:614-22).
  • EP 1 , 176, 195 by Hanai et al. describes a cell line with a functionally disrupted FUT8 gene, which encodes a fuc ⁇ sv i transferase, such that antibodies expressed in such a cell line exhibit hypo fixcosylat ion by reducing or eliminating the alpha 1,6 bond-related enzyme.
  • Hanai et al. also describe cell lines which have a low enzyme activity for adding fucose to the N-acetylglucosamine that binds to the Fc region of the antibody or does not have the enzyme activity, for example the rat myeloma cell line YB2/0 (ATCC CRL 1662).
  • PCT Publication WO 03/035835 by Presta describes a variant CHO cell line, Lee 13 cells, with reduced ability to attach fucose to Asn(297)-linked carbohydrates, also resulting in hypofucosylation of antibodies expressed in that host cell (see also Shields, R.L. et al. (2002) J. Biol. Client. 277:26733-26740).
  • PCT Publication WO 99/54342 by Umana et al.
  • glycoprotein-modifying glycosyl transferases ⁇ e.g., beta( l,4)-N- acetylglucosaminyltransferase III (GnTIII)
  • GnTIII glycoprotein-modifying glycosyl transferases
  • the fucose residues of the antibody may be cleaved off using a fucosidase enzyme.
  • the fucosidase alpha-L-fucosidase removes fucosyl residues from antibodies (Tarentino, A.
  • an antibody can be made that has an altered type of glycosylation, wherein that alteration relates to the level of sialyation of the antibody.
  • Such alterations are described in PCT Publication No. WO/2007/084926 to Dickey et al , and PCT Publication No. WO/2007/055916 to Ravetch et al., both of which are incorporated by reference in their entirety.
  • sialidase such as, for example, Arthrobacter ureafacens sialidase. The conditions of such a reaction are generally described in the U.S. Patent No.
  • Suitable enzymes are neuraminidase and N-GIycosidase F, as described in Schloemer et al . , ]. Virology, 15(4), 882-893 (1975) and in Leibiger et al . , Biochem J., 338, 529-538 (1999), respectively. Desialylated antibodies may be further purified by using affinity chromatography. Alternatively, one may employ methods to increase the level of sialyation, such as by employing sialytransferase enzymes. Conditions of such a reaction are generally described in Basset et al., Scandinavian Journal of Immunology, 51(3), 307-31 1 (2000).
  • An antibody can be pegylated to, for example, increase the biological (e.g., serum) half life of the antibody.
  • the antibody, or fragment thereof typically is reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions in which one or more PEG groups become attached to the antibody or antibody fragment.
  • PEG polyethylene glycol
  • the pegylation is earned out via an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous reactive water-soluble polymer).
  • polyethylene glycol is intended to encompass any of the forms of PEG that have been used to derivatize other proteins, such as mono (Cl-Cl 0) alkoxy- or aryloxy- polyethylene glycol or polyethylene glycol-maleirnide.
  • the antibody to be pegylated is an aglycosylated antibody. Methods for pegylating proteins are known in the art and can be applied to the antibodies of this disclosure. See for example, EP 0 154 316 by Nishimura et al. and EP 0401 384 by Ishikawa et al.
  • Antibody Fragments and Antibody Mimetics The instant invention is not limited to traditional antibodies and may be practiced through the use of antibody fragments and antibody mimetics.
  • antibody fragment and antibody mimetic technologies have now been developed and are widely known in the art. While a number of these technologies, such as domain antibodies, Nanobodies, and UniBodies make use of fragments of, or other modifications to, traditional antibody structures, there are also alternative technologies, such as Affibodies, DARPins, Anticalins, Avimers, and Versabodies that employ binding structures that, while they mimic traditional antibody binding, are generated from and function via distinct mechanisms.
  • Domain Antibodies are the smallest functional binding units of antibodies, corresponding to the variable regions of either the heavy (VH) or light (VL) chains of human antibodies. Domain Antibodies have a molecular weight of approximately 13 kDa. Domantis has developed a series of large and highly functional libraries of fully human VH and VL dAbs (more than ten billion different sequences in each library), and uses these libraries to select dAbs that are specific to therapeutic targets. In contrast to many conventional antibodies, Domain Antibodies are well expressed in bacterial, yeast, and mammalian cell systems. Further details of domain antibodies and methods of production thereof may be obtained by reference to U.S.
  • Nanobodies are antibody-derived therapeutic proteins that contain the unique structural and functional properties of naturally-occurring heavy-chain antibodies. These heavy-chain antibodies contain a single variable domain (VHH) and two constant domains (CH2 and CH3). Importantly, the cloned and isolated VHH domain is a perfectly stable polypeptide harboring the full antigen-binding capacity of the original heavy-chain antibody. Nanobodies have a high homology with the VH domains of human antibodies and can be further humanized without any loss of activity. Importantly, Nanobodies have a low immunogenic potential, which has been confirmed in primate studies with Nanobody lead compounds. Nanobodies combine the advantages of conventional antibodies with important features of small molecule drags.
  • Nanobodies Like conventional antibodies, Nanobodies show high target specificity, high affinity for their target and low inherent toxicity. However, like small molecule drugs they can inhibit enzymes and readily access receptor clefts. Furthermore, Nanobodies are extremely stable, can be administered by means other than injection (see, e.g., WO 04/041867, which is herein incoiporated by reference in its entirety) and are easy to manufacture. Other advantages of Nanobodies include recognizing uncommon or hidden epitopes as a result of their small size, binding into cavities or active sites of protein targets with high affinity and selectivity due to their unique 3 -dimensional, drug format flexibility, tailoring of half-life and ease and speed of drug discovery.
  • Nanobodies are encoded by single genes and are efficiently produced in almost all prokaryotic and eukaryotic hosts, e.g., E. coli (see, e.g., U.S. 6,765,087, which is herein incorporated by reference in its entirety), molds (for example Aspergillus or Trichoderma) and yeast (for example Saccharomyces, Kluyveromyces, Hansenula or Pichia) (see, e.g., U.S. 6,838,254, which is herein incoiporated by reference in its entirety).
  • the production process is scalable and multi-kilogram quantities of
  • Nanobodies have been produced. Because Nanobodies exhibit a superior stability compared with conventional antibodies, they can be formulated as a long shelf-life, ready-to-use solution.
  • the Nanoclone method (see, e.g., WO 06/079372, which is herein incoiporated by reference in its entirety) is a proprietary method for generating Nanobodies against a desired target, based on automated high-throughout selection of B-cells and could be used in the context of the instant invention.
  • UniBodies are another antibody fragment technology, however this one is based upon the removal of the hinge region of IgG4 antibodies. The deletion of the hinge region results in a molecule that is essentially half the size of traditional IgG4 antibodies and has a univalent binding region rather than the bivalent binding region of IgG4 antibodies. It is also well known that IgG4 antibodies are inert and thus do not interact with the immune system, which may be advantageous for the treatment of diseases where an immune response is not desired, and this advantage is passed onto UniBodies. For example, UniBodies may function to inhibit or silence, but not kill, the cells to which they are bound. Additionally, UniBody binding to cancer cells do not stimulate them to proliferate.
  • UniBodies are about half the size of traditional IgG4 antibodies, they may show better distribution over larger solid tumors with potentially advantageous efficacy. UniBodies are cleared from the body at a similar rate to whole IgG4 antibodies and are able to bind with a similar affinity for their antigens as whole antibodies. Further details of UniBodies may be obtained by reference to patent application WO2007/059782, which is herein incorporated by reference in its entirety.
  • Affibody molecules represent a new class of affinity proteins based on a 58-amino acid residue protein domain, derived from one of the igG-binding domains of staphylococcal protein A. This three helix bundle domain has been used as a scaffold for the construction of combinatorial phagemid libraries, from which Affibody valiants that target the desired molecules can be selected using phage display technology (Nord K, Gunneriusson E, Ringdahl J, Stahl S, Uhlen M, Nygren PA, Binding proteins selected from combinatorial libraries of an ⁇ -helical bacterial receptor domain, Nat Biotechnol 1997; 15:772-7.
  • Affibody molecules in combination with their low molecular weight (6 kDa), make them suitable for a wide variety of applications, for instance, as detection reagents (Ronmark J, Hansson M, Nguyen T, et al, Construction and characterization of affibody-Fc chimeras produced in Escherichia coli, J Immunol Methods 2002;261 : 199-21 1 ) and to inhibit receptor interactions (Sandstorai K, Xu Z, Forsberg G, Nygren PA, Inhibition of the CD28-CD80 co-stimulation signal by a CD28-binding Affibody ligand developed by combinatorial protein engineering, Protein Eng 2003;16:691-7). Further details of Affi
  • Labeled Affibodies may also be useful in imaging applications for determining abundance of Isoforms.
  • DARPins Designed Ankyrin Repeat Proteins
  • Repeat proteins such as ankyrin or leucine-rich repeat proteins, are ubiquitous binding molecules, which occur, unlike antibodies, intra- and extracellularly.
  • Their unique modular architecture features repeating structural units (repeats), which stack together to form elongated repeat domains displaying variable and modular target-binding surfaces. Based on this modularity, combinatorial libraries of polypeptides with highly diversified binding specificities can be generated. This strategy includes the consensus design of self- compatible repeats displaying variable surface residues and their random assembly into repeat domains.
  • DARPins can be produced in bacterial expression systems at very high yields and they belong to the most stable proteins known. Highly specific, high-affinity DARPins to a broad range of target proteins, including human receptors, cytokines, kinases, human proteases, viruses and membrane proteins, have been selected. DARPins having affinities in the single-digit nanomolar to picomolar range can be obtained.
  • DARPins have been used in a wide range of applications, including ELISA, sandwich ELISA, flow cytometric analysis (FACS), immunohistochemistry (IHC), chip applications, affinity purification or Western blotting. DARPins also proved to be highly active in the intracellular compartment for example as intracellular marker proteins fused to green fluorescent protein (GFP). DARPins were further used to inhibit viral entry with IC50 in the pM range. DARPins are not only ideal to block protein-protein interactions, but also to inhibit enzymes. Proteases, kinases and transporters have been successfully inhibited, most often an allosteric inhibition mode. Very fast and specific enrichments on the tumor and very favorable tumor to blood ratios make DARPins well suited for in vivo diagnostics or therapeutic approaches.
  • Anticalins are an additional antibody mimetic technology, however in this case the binding specificity is derived from lipocalins, a family of low molecular weight proteins that are naturally and abundantly expressed in human tissues and body fluids. Lipocalins have evolved to perform a range of functions in vivo associated with the physiological transport and storage of chemically sensitive or insoluble compounds. Lipocalins have a robust intrinsic structure comprising a highly conserved ⁇ -barrel which supports four loops at one terminus of the protein. These loops form the entrance to a binding pocket and conformational differences in this part of the molecule account for the variation in binding specificity between individual lipocalins.
  • lipocalins differ considerably from antibodies in terms of size, being composed of a single polypeptide chain of 160-180 amino acids which is marginally larger than a single immunoglobulin domain.
  • Lipocalins are cloned and their loops are subjected to engineering in order to create Anticalins. Libraries of structurally diverse Anticalins have been generated and Anticalin display allows the selection and screening of binding function, followed by the expression and production of soluble protein for further analysis in prokaryotic or eukaryotic systems. Studies have successfully demonstrated that Anticalins can be developed that are specific for virtually any human target protein can be isolated and binding affinities in the nanomolar or higher range can be obtained.
  • Anticalins can also be formatted as dual targeting proteins, so-called Duocalins.
  • a Duocalin binds two separate therapeutic targets in one easily produced monomeric protein using standard manufacturing processes while retaining target specificity and affinity regardless of the structural orientation of its two binding domains. Modulation of multiple targets through a single molecule is particularly advantageous in diseases known to involve more than a single causative factor.
  • bi- or multivalent binding formats such as Duocalins have significant potential in targeting cell surface molecules in disease, mediating agonistic effects on signal transduction pathways or inducing enhanced internalization effects via binding and clustering of cell surface receptors.
  • the high intrinsic stability of Duocalins is comparable to monomeric Anticalins, offering flexible formulation and delivery potential for Duocalins.
  • Anticalins can be found in U.S. Patent No. 7,250,297 and International Patent Application Publication No. WO 99/16873, both of which are hereby incorporated by reference in their entirety.
  • Another antibody mimetic technology useful in the context of the instant invention are Avimers.
  • Avimers are evolved from a large family of human extracellular receptor domains by in vitro exon shuffling and phage display, generating multidomain proteins with binding and inhibitory properties. Linking multiple independent binding domains has been shown to create avidity and results in improved affinity and specificity compared with conventional single-epitope binding proteins.
  • Other potential advantages include simple and efficient production of multitarget-specific molecules in Escherichia coli, improved thermostability and resistance to proteases.
  • Avimers with sub-nanomolar affinities have been obtained against a variety of targets. Additional information regarding Avimers can be found in U.S. Patent
  • Versabodies are another antibody mimetic technology that could be used in the context of the instant invention.
  • Versabodies are small proteins of 3-5 kDa with >15% cysteines, which form a high disulfide density scaffold, replacing the hydrophobic core that typical proteins have.
  • antibody fragment and antibody mimetic technologies are not intended to be a comprehensive list of all technologies that could be used in the context of the instant specification.
  • additional technologies including alternative polypeptide-based technologies, such as fusions of complimentary determining regions as outlined in Qui et al., Nature Biotechnology, 25(8) 921-929 (2007), which is hereby incorporated by reference in its entirety, as well as nucleic acid-based technologies, such as the RNA aptamer technologies described in U.S. Patent Nos.
  • the antibodies of the present disclosure may be further characterized by the various physical properties of the anti-CD 19 antibodies.
  • Various assays may be used to detect and/or differentiate different classes of antibodies based on these physical properties.
  • antibodies of the present disclosure may contain one or more glycosylation sites in either the light or heavy chain variable region.
  • the presence of one or more glycosylation sites in the variable region may result in increased immunogenicity of the antibody or an alteration of the pK of the antibody due to altered antigen binding (Marshall et al (1972) Annu Rev Biochem 41:673-702; Gala FA and Morrison SL (2004) J Immunol 172:5489-94; Wallick et al (1988) J Exp Med 168: 1099- 109; Spiro RG (2002) Glycobiology I2:43R-56R; Parekh et al (1985) Nature 316:452-7; Mimura et al.
  • variable region glycosylation may be tested using a Glycoblot assay, which cleaves the antibody to produce a Fab, and then tests for glycosylation using an assay that measures periodate oxidation and Schiff base formation.
  • variable region glycosylation may be tested using Dionex light chromatography (Dionex-LC), which cleaves saccharides from a Fab into monosaccharides and analyzes the individual saccharide content.
  • Dionex-LC Dionex light chromatography
  • the antibodies of the present disclosure do not contain asparagine isomerism sites.
  • a deamidation or isoaspartic acid effect may occur on N-G or D-G sequences, respectively.
  • the deamidation or isoaspartic acid effect results in the creation of isoaspartic acid which decreases the stability of an antibody by creating a kinked structure off a side chain carboxy terminus rather than the main chain.
  • the creation of isoaspartic acid can be measured using an iso-quant assay, which uses a reverse-phase HPLC to test for isoaspartic acid.
  • Each antibody will have a unique isoelectric point (pi), but generally antibodies will fall in the pH range of between 6 and 9.5.
  • the pi for an IgGl antibody typically falls within the pH range of 7-9.5 and the pi for an IgG4 antibody typically falls within the pH range of 6-8.
  • Antibodies may have a pi that is outside this range. Although the effects are generally unknown, there is speculation that antibodies with a pi outside the normal range may have some unfolding and instability under in vivo conditions.
  • the isoelectric point may be tested using a capillary isoelectric focusing assay, which creates a pH gradient and may utilize laser focusing for increased accuracy (Janini et al (2002) Electrophoresis 23: 1605-1 1 ; Ma et al.
  • an anti- CD 19 antibody that contains a pi value that falls in the normal range. This can be achieved either by selecting antibodies with a pi in the normal range, or by mutating charged surface residues using standard techniques well known in the art.
  • each antibody will have a melting temperature that is indicative of thermal stability (Krishnamurthy R and Manning MC (2002) Curr Pharm Biotechnol 3:361-71). A higher thermal stability indicates greater overall antibody stability in vivo.
  • the melting point of an antibody may be measured using techniques such as differential scanning calorimetry (Chen et al (2003) Pharm Res 20: 1952-60; Ghirlando et al (1999) Immunol Lett 68:47-52).
  • T M I indicates the temperature of the initial unfolding of the antibody.
  • T M2 indicates the temperature of complete unfolding of the antibody.
  • the T M i of an antibody of the present disclosure is greater than 60 0 C, preferably greater than 65°C, even more preferably greater than 7O 0 C.
  • the thermal stability of an antibody may be measured using circular dichroism (Murray et al. (2002) J. Chromatogr Sci 40:343-9).
  • antibodies are selected that do not rapidly degrade. Fragmentation of an anti-CD 19 antibody may be measured using capillary electrophoresis (CE) and MALDI-MS, as is well understood in the art (Alexander AJ and Hughes DE (1995) Anal Chem 67:3626-32).
  • CE capillary electrophoresis
  • MALDI-MS MALDI-MS
  • antibodies are selected that have minimal aggregation effects. Aggregation may lead to triggering of an unwanted immune response and/or altered or unfavorable pharmacokinetic properties. Generally, antibodies are acceptable with aggregation of 25% or less, preferably 20% or less, even more preferably 15% or less, even more preferably 10% or less and even more preferably 5% or less. Aggregation may be measured by several techniques well known in the art, including size-exclusion column (SEC) high performance liquid chromatography (HPLC), and light scattering to identify monomers, dinners, trimers or multimers.
  • SEC size-exclusion column
  • HPLC high performance liquid chromatography
  • the anti-CD 19 antibodies having V H and V K sequences disclosed herein can be used to create new anti-CD 19 antibodies by modifying the V H and/or V K sequences, or the constant region(s) attached thereto.
  • the staictural features of an anti-CD 19 antibody of this disclosure e.g. 21 D4, 21 D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8, are used to create structurally related anti-CD 19 antibodies that retain at least one functional property of the antibodies of this disclosure, such as binding to human CD 19.
  • one or more CDR regions of 21 D4, 21 D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl or 46E8, or mutations thereof can be combined recombinantly with known framework regions and/or other CDRs to create additional, recombinantly-engineered, anti-CD 19 antibodies of this disclosure, as discussed above.
  • Other types of modifications include those described in the previous section.
  • the starting material for the engineering method is one or more of the V H and'or V K sequences provided herein, or one or more CDR regions thereof.
  • this disclosure provides a method for preparing an anti-CD 19 antibody comprising:
  • the altered antibody sequence as a protein.
  • Standard molecular biology techniques can be used to prepare and express the altered antibody sequence.
  • the antibody encoded by the altered antibody sequence(s) is one that retains one, some or all of the functional properties of the anti-CD 19 antibodies described herein, which functional properties include, but are not limited to:
  • the functional properties of the altered antibodies can be assessed using standard assays available in the art and/or described herein, such as those set forth in the Examples (e.g.. flow cytometry, binding assays).
  • mutations can be introduced randomly or selectively along all or part of an anti-CD 19 antibody coding sequence and the resulting modified anti-CD 19 antibodies can be screened for binding activity and/or other functional properties as described herein.
  • Mutational methods have been described in the art.
  • PCT Publication WO 02/092780 by Short describes methods for creating and screening antibody mutations using saturation mutagenesis, synthetic ligation assembly, or a combination thereof.
  • PCT Publication WO 03/074679 by Lazar et al. describes methods of using computational screening methods to optimize physiochemical properties of antibodies.
  • nucleic Acid Molecules Encoding Antibodies of this Disclosure Another aspect of this disclosure pertains to nucleic acid molecules that encode the antibodies of this disclosure.
  • the nucleic acids may be present in whole cells, in a cell lysate, or in a partially purified or substantially pure form.
  • a nucleic acid is "isolated” or “rendered substantially pure” when purified away from other cellular components or other contaminants, e.g., other cellular nucleic acids or proteins, by standard techniques, including alkaline/SDS treatment, CsCl banding, column chromatography, agarose gel electrophoresis and others well known in the art. See, F. Ausubel, et ai, ed.
  • a nucleic acid of this disclosure can be, for example, DNA or RNA and may or may not contain intronic sequences.
  • the nucleic acid is a cDNA molecule.
  • Nucleic acids of this disclosure can be obtained using standard molecular biology techniques. For antibodies expressed by hybridomas (e.g., hybridomas prepared from transgenic mice carrying human immunoglobulin genes as described further below), cDNAs encoding the light and heavy chains of the antibody made by the hybridoma can be obtained by standard PCR amplification or cDNA cloning techniques. For antibodies obtained from an immunoglobulin gene library (e.g., using phage display techniques), one or more nucleic acids encoding the antibody can be recovered from the library.
  • hybridomas e.g., hybridomas prepared from transgenic mice carrying human immunoglobulin genes as described further below
  • cDNAs encoding the light and heavy chains of the antibody made by the hybridoma can be obtained by standard PCR amplification or cDNA clon
  • Preferred nucleic acids molecules of this disclosure are those encoding the Vn and V L sequences of the 21 D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 monoclonal antibodies.
  • DNA sequences encoding the Vn sequences of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 59, 60, 61, 62, 63, 64 and 65, respectively.
  • DNA sequences encoding the V L sequences of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 66, 67, 68, 69, 70, 71, 72 and 73, respectively.
  • Vn and V L segments are obtained, these DNA fragments can be further manipulated by standard recombinant DNA techniques, for example to convert the variable region genes to full-length antibody chain genes, to Fab fragment genes or to a scFv gene.
  • a V L - or Vn-encoding DNA fragment is operatively linked to another DNA fragment encoding another protein, such as an antibody constant region or a flexible linker.
  • the term "operatively linked”, as used in this context, is intended to mean that the two DNA fragments are joined such that the amino acid sequences encoded by the two DNA fragments remain in-frame.
  • the isolated DNA encoding the V H region can be converted to a full-length heavy chain gene by operatively linking the VH-encoding DNA to another DNA molecule encoding heavy chain constant regions (CHl, CH2 and CH3).
  • CHl heavy chain constant regions
  • the sequences of human heavy chain constant region genes are known in the art (see e.g., Kabat, E. A., el al.
  • the heavy chain constant region can be an IgGl, IgG2, IgG3, IgG4, IgA, IgE, IgM or IgD constant region, but most preferably is an IgG 1 or IgG4 constant region.
  • the V ⁇ -encoding DNA can be operatively linked to another DNA molecule encoding only the heavy chain CH 1 constant region.
  • the isolated DNA encoding the V L region can be converted to a full-length light chain gene (as well as a Fab light chain gene) by operatively linking the V L -encoding DNA to another DNA molecule encoding the light chain constant region, CL.
  • the sequences of human light chain constant region genes are known in the art (see e.g., Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242) and DNA fragments encompassing these regions can be obtained by standard PCR amplification.
  • the light chain constant region can be a kappa or lambda constant region.
  • V H - and V t -encoding DNA fragments are operatively linked to another fragment encoding a flexible linker, e.g., encoding the amino acid sequence (GIy 4 -Ser) 3 , such that the V H and V] sequences can be expressed as a contiguous single-chain protein, with the V 1 and V H regions joined by the flexible linker (see e.g., Bird et al. (1988) Science 242:423-426; Huston et al. (1988) Proc. Natl. Acad. ScL USA 85:5879-5883; McCafferty et al., (1990) Nature 348:552-554).
  • a flexible linker e.g., encoding the amino acid sequence (GIy 4 -Ser) 3 , such that the V H and V] sequences can be expressed as a contiguous single-chain protein, with the V 1 and V H regions joined by the flexible linker (see e.g., Bird
  • Monoclonal antibodies (mAbs) of the present disclosure can be produced by a variety of techniques, including conventional monoclonal antibody methodology e.g., the standard somatic cell hybridization technique of Kohler and Milstein (1975) Nature 256:
  • hybridomas The preferred animal system for preparing hybridomas is the murine system.
  • Hybridoma production in the mouse is a very well-established procedure. Immunization protocols and techniques for isolation of immunized splenocytes for fusion are known in
  • Fusion partners e.g., murine myeloma cells
  • fusion procedures are also known.
  • Chimeric or humanized antibodies of the present disclosure can be prepared based on the sequence of a non-human monoclonal antibody prepared as described above.
  • DNA encoding the heavy and light chain immunoglobulins can be obtained from the non- human hybridoma of interest and engineered to contain non-murine (e.g., human) immunoglobulin sequences using standard molecular biology techniques.
  • murine variable regions can be linked to human constant regions using methods known in the art (see e.g., U.S. Patent No. 4,816,567 to Cabilly et a L).
  • murine CDR regions can be inserted into a human framework using methods known in the art (see e.g., U.S. Patent No. 5,225,539 to Winter, and U.S. Patent Nos. 5,530,101 ; 5,585,089; 5,693,762 and 6, 180,370 to Queen et al.).
  • the antibodies of this disclosure are human monoclonal antibodies.
  • Such human monoclonal antibodies directed against CD 19 can be generated using transgenic or transchromosomic mice carrying parts of the human immune system rather than the mouse system.
  • These transgenic and transchromosomic mice include mice referred to herein as the HuMAb Mouse* and KM Mouse* ' , respectively, and are collectively referred to herein as '"human Ig mice.”
  • the HuMAb Mouse* (Medarex ® , Inc.) contains human immunoglobulin gene miniloci that encode unrearranged human heavy ( ⁇ and ⁇ ) and K light chain immunoglobulin sequences, together with targeted mutations that inactivate the endogenous ⁇ and K chain loci (see e.g., Lonberg, et al.
  • mice exhibit reduced expression of mouse IgM or K, and in response to immunization, the introduced human heavy and light chain transgenes undergo class switching and somatic mutation to generate high affinity human IgG ⁇ monoclonal antibodies (Lonberg, N. et al. (1994), supra; reviewed in Lonberg, N. (1994) Handbook of Experimental Pharmacology 113 :49- 101 ; Lonberg, N. and Huszar, D. (1995) Intern. Rev. Immunol. L3: 65-93, and Harding, F. and Lonberg, N. (1995) Ann. N. Y. Acad. Sci. 764:536-546).
  • a mouse carrying a human lambda light chain transgene can be crossbred with a mouse carrying a human heavy chain transgene (e.g., HCo7), and optionally also carrying a human kappa light chain transgene (e.g., KCo5) to create a mouse carrying both human heavy and light chain transgenes (see e.g.. Example 1).
  • a human heavy chain transgene e.g., HCo7
  • a human kappa light chain transgene e.g., KCo5
  • human antibodies of this disclosure can be raised using a mouse that carries human immunoglobulin sequences on transgenes and transchomosomes, such as a mouse that carries a human heavy chain transgene and a human light chain transchromosome.
  • This mouse is referred to herein as a '"KM mouse ® ", and is described in detail in PCT Publication WO 02/43478 to Ishida et al.
  • transgenic animal systems expressing human immunoglobulin genes are available in the art and can be used to raise anti-CD 19 antibodies of this disclosure.
  • an alternative transgenic system referred to as the Xenomouse (Abgenix, Inc.) can be used; such mice are described in, for example, U.S. Patent Nos. 5,939,598; 6,075, 181; 6,114,598; 6,150,584 and 6, 162,963 to Kucherlapati et al.
  • mice carrying both a human heavy chain transchromosome and a human light chain tranchromosome referred to as "TC mice” can be used; such mice are described in Tomizuka et al. (2000) Proc. Natl. Acad. Sci. USA 92:122-121.
  • cows carrying human heavy and light chain transchromosomes have been described in the art ⁇ e.g., Kuroiwa et al. (2002) Nature Biotechnology 20:889-894 and PCT application No. WO 2002/092812) and can be used to raise anti-CD 19 antibodies of this disclosure.
  • Human monoclonal antibodies of this disclosure can also be prepared using phage display methods for screening libraries of human immunoglobulin genes.
  • phage display methods for isolating human antibodies are established in the art. See for example: U.S. Patent Nos. 5,223,409; 5,403,484; and 5,571,698 to Ladner et al; U.S. Patent Nos. 5,427,908 and 5,580,717 to Dower et al.; U.S. Patent Nos. 5,969,108 and 6,172,197 to McCafferty et al.; and U.S. Patent Nos. 5,885,793; 6,521,404; 6,544,731; 6,555,313; 6,582,9130, 6,582,915 and 6,593,081 to Griffiths et al.
  • Human monoclonal antibodies of this disclosure can also be prepared using SCID mice into which human immune cells have been reconstituted such that a human antibody response can be generated upon immunization.
  • SCID mice into which human immune cells have been reconstituted such that a human antibody response can be generated upon immunization.
  • Such mice are described in, for example, U.S. Patent Nos. 5,476,996 and 5,698,767 to Wilson et al.
  • human anti-CD 19 antibodies are prepared using a combination of human Ig mouse and phage display techniques, as described in U.S. Patent No. 6,794,132 by Buechler et al. More specifically, the method first involves raising an anti-CD 19 antibody response in a human Ig mouse (such as a HuMab mouse or KM mouse as described above) by immunizing the mouse with one or more CD 19 antigens, followed by isolating nucleic acids encoding human antibody chains from lymphatic cells of the mouse and introducing these nucleic acids into a display vector ⁇ e.g., phage) to provide a library of display packages.
  • a human Ig mouse such as a HuMab mouse or KM mouse as described above
  • each library member comprises a nucleic acid encoding a human antibody chain and each antibody chain is displayed from the display package.
  • the library then is screened with CD 19 protein to isolate library members that specifically bind to CD 19.
  • Nucleic acid inserts of the selected library members then are isolated and sequenced by standard methods to determine the light and heavy chain variable sequences of the selected CD 19 binders.
  • the variable regions can be converted to full-length antibody chains by standard recombinant DNA techniques, such as cloning of the variable regions into an expression vector that carries the human heavy and light chain constant regions such that the Vn region is operatively linked to the Cn region and the V 1 region is operatively linked to the C L region.
  • mice When human Ig mice are used to raise human antibodies of this disclosure, such mice can be immunized with a purified or enriched preparation of CD 19 antigen and/or recombinant CD 19, or cells expressing a CD 19 protein, or a CD 19 fusion protein, as described by Lonberg, N. et al. (1994) Nature 368(6474): 856-859; Fishwild, D. et al. (1996) Nature Biotechnology W: 845-851 ; and PCT Publication WO 98/24884 and WO 01/14424.
  • the mice will be 6-16 weeks of age upon the first infusion.
  • a purified or recombinant preparation (5-50 ⁇ g) of CD 19 antigen can be used to immunize the human Ig mice intraperitoneally and/or subcutaneously.
  • the immunogen used to raise the antibodies of this disclosure is a CD 19 fusion protein comprising the extracellular domain of a CD 19 protein, fused at its N-terminus to a non- CD ⁇ polypeptide (e.g., a His tag) (described further in Example 1).
  • Example I Detailed procedures to generate fully human monoclonal antibodies that bind to human CD 19 are described in Example I below. Cumulative experience with various antigens has shown that the transgenic mice respond when initially immunized intraperitoneally (IP) with antigen in complete Freund's adjuvant, followed by every other week IP immunizations (up to a total of 6) with antigen in incomplete Freund's adjuvant. However, adjuvants other than Freund's are also found to be effective (e.g., RIBI adjuvant). In addition, whole cells in the absence of adjuvant are found to be highly immunogenic. The immune response can be monitored over the course of the immunization protocol with plasma samples being obtained by retroorbital bleeds.
  • mice with sufficient titers of anti-CD 19 human immunoglobulin can be used for fusions.
  • Mice can be boosted intravenously with antigen, for example 3 days before sacrifice and removal of the spleen. It is expected that 2-3 fusions for each immunization may need to be performed. Between 6 and 24 mice are typically immunized for each antigen.
  • HCo7 and HCo 12 strains are used.
  • both HCo7 and HCo 12 transgene can be bred together into a single mouse having two different human heavy chain transgenes (HCo7/HCol2).
  • the KM Mouse 1 " strain can be used..
  • splenocytes and/or lymph node cells from immunized mice can be isolated and fused to an appropriate immortalized cell line, such as a mouse myeloma cell line.
  • an appropriate immortalized cell line such as a mouse myeloma cell line.
  • the resulting hybridomas can be screened for the production of antigen-specific antibodies.
  • single cell suspensions of splenic lymphocytes from immunized mice can be fused to one-sixth the number of P3X63-Ag8.653 nonsecreting mouse myeloma cells (ATCC, CRL 1580) with 50% PEG.
  • the single cell suspension of splenic lymphocytes from immunized mice can be fused using an electric field based electrofusion method, using a CytoPulse large chamber cell fusion electroporator (CytoPulse Sciences, Inc., Glen Burnie Maryland).
  • Cells are plated at approximately 2 x 10 in flat bottom microti ter plate, followed by a two week incubation in selective medium containing 20% fetal Clone Serum, 18% "653" conditioned media, 5% origen (IGEN), 4 mM L-glutamine, 1 mM sodium pyruvate, 5mM HEPES, 0.055 mM 2-mercaptoethanol, 50 units/ml penicillin, 50 mg/ml streptomycin, 50 mg/ml gentamycin and IX HAT (Sigma; the HAT is added 24 hours after the fusion). After approximately two weeks, cells can be cultured in medium in which the HAT is replaced with HT.
  • selective medium containing 20% fetal Clone Serum, 18% "653" conditioned media, 5% origen (IGEN), 4 mM L-glutamine, 1 mM sodium pyruvate, 5mM HEPES, 0.055 mM 2-mercaptoethanol, 50 units/ml penicillin,
  • selected hybridomas can be grown in two-liter spinner-flasks for monoclonal antibody purification.
  • Supernatants can be filtered and concentrated before affinity chromatography with protein A-sepharose (Pharmacia, Piscataway, NJ.).
  • Eluted IgG can be checked by gel electrophoresis and high performance liquid chromatography to ensure purity.
  • the buffer solution can be exchanged into PBS, and the concentration can be determined by OD280 using 1.43 extinction coefficient.
  • the monoclonal antibodies can be aliquoted and stored at -80° C.
  • Antibodies of this disclosure also can be produced in a host cell transfectoma using, for example, a combination of recombinant DNA techniques and gene transfection methods as is well known in the art (e.g., Morrison, S. (1985) Science 229: 1202).
  • DNAs encoding partial or full-length light and heavy chains can be obtained by standard molecular biology techniques (e.g., PCR amplification or cDNA cloning using a hybridoma that expresses the antibody of interest) and the DNAs can be inserted into expression vectors such that the genes are operatively linked to transcriptional and translational control sequences.
  • operatively linked is intended to mean that an antibody gene is ligated into a vector such that transcriptional and translational control sequences within the vector serve their intended function of regulating the transcription and translation of the antibody gene.
  • the expression vector and expression control sequences are chosen to be compatible with the expression host cell used.
  • the antibody light chain gene and the antibody heavy chain gene can be inserted into separate vector or, more typically, both genes are inserted into the same expression vector.
  • the antibody genes are inserted into the expression vector by standard methods (e.g., ligation of complementary restriction sites on the antibody gene fragment and vector, or blunt end ligation if no restriction sites are present).
  • the light and heavy chain variable regions of the antibodies described herein can be used to create full-length antibody genes of any antibody isotype by inserting them into expression vectors already encoding heavy chain constant and light chain constant regions of the desired isotype such that the V H segment is operatively linked to the C M segment(s) within the vector and the V K segment is operatively linked to the C 1 . segment within the vector.
  • the recombinant expression vector can encode a signal peptide that facilitates secretion of the antibody chain from a host cell.
  • the antibody chain gene can be cloned into the vector such that the signal peptide is linked in-frame to the amino terminus of the antibody chain gene.
  • the signal peptide can be an immunoglobulin signal peptide or a heterologous signal peptide (i.e., a signal peptide from a non- immunoglobulin protein).
  • the recombinant expression vectors of this disclosure carry regulatory sequences that control the expression of the antibody chain genes in a host cell.
  • the term "regulatory sequence” is intended to include promoters, enhancers and other expression control elements (e.g., polyadenylation signals) that control the transcription or translation of the antibody chain genes.
  • Such regulatory sequences are described, for example, in Goeddel (Gene Expression Technology. Methods in Enzymology 185, Academic Press, San Diego, CA (1990)). It will be appreciated by those skilled in the art that the design of the expression vector, including the selection of regulatory sequences, may depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc.
  • Preferred regulatory sequences for mammalian host cell expression include viral elements that direct high levels of protein expression in mammalian cells, such as promoters and/or enhancers derived from cytomegalovirus (CMV), Simian Virus 40 (SV40), adenovirus, (e.g., the adenovirus major late promoter (AdMLP)) and polyoma.
  • CMV cytomegalovirus
  • SV40 Simian Virus 40
  • AdMLP adenovirus major late promoter
  • nonviral regulatory sequences may be used, such as the ubiquitin promoter or ⁇ -globin promoter.
  • regulatory elements composed of sequences from different sources such as the SRa promoter system, " which contains sequences from the SV40 early promoter and the long terminal repeat of human T cell leukemia virus type 1 (Takebe, Y. et al. (1988) MoL Cell. Biol. 8:466-472).
  • the recombinant expression vectors of this disclosure may carry additional sequences, such as sequences that regulate replication of the vector in host cells (e.g., origins of replication) and selectable marker genes.
  • the selectable marker gene facilitates selection of host cells into which the vector has been introduced (see, e.g., U.S. Pat. Nos. 4,399,216, 4,634,665 and 5,179,017, all by Axel et al ).
  • the selectable marker gene confers resistance to drugs, such as G418, hygromycin or methotrexate, on a host cell into which the vector has been introduced.
  • Preferred selectable marker genes include the dihydrofolate reductase (DHFR) gene (for use in dhfr- host cells with methotrexate selection/amplification) and the neo gene (for G418 selection).
  • DHFR dihydrofolate reductase
  • neo gene for G418 selection.
  • the expression vector(s) encoding the heavy and light chains is transfected into a host cell by standard techniques.
  • the various forms of the term "transfection" are intended to encompass a wide variety of techniques commonly used for the introduction of exogenous DNA into a prokaryotic or eukaryotic host cell, e.g., electroporation, calcium-phosphate precipitation, DEAE- dextran transfection and the like.
  • Preferred mammalian host cells for expressing the recombinant antibodies of this disclosure include Chinese Hamster Ovary (CHO cells) (including dhfr ' CHO cells, described in Urlaub and Chasin, (1980) Proc. Natl. Acad. Sci. USA 77:4216-4220, used with a DHFR selectable marker, e.g., as described in R. J. Kaufman and P. A. Sharp (1982) J. MoL Biol. 159:601-621), NSO myeloma cells, COS cells and SP2 cells.
  • Chinese Hamster Ovary CHO cells
  • dhfr ' CHO cells described in Urlaub and Chasin, (1980) Proc. Natl. Acad. Sci. USA 77:4216-4220, used with a DHFR selectable marker, e.g., as described in R. J. Kaufman and P. A. Sharp (1982) J. MoL Biol. 159:601-621
  • another preferred expression system is the GS gene expression system disclosed in WO 87/04462 (to Wilson), WO 89/01036 (to Bebbington) and EP 338,841 (to Bebbington).
  • the antibodies are produced by culturing the host cells for a period of time sufficient to allow for expression of the antibody in the host cells or, more preferably, secretion of the antibody into the culture medium in which the host cells are grown.
  • Antibodies can be recovered from the culture medium using standard protein purification methods.
  • Antibodies of this disclosure can be tested for binding to human CD 19 by, for example, standard ELISA. Briefly, microtiter plates are coated with purified at 0.25 ⁇ g/ml in PBS, and then blocked with 5% bovine serum albumin in PBS. Dilutions of antibody (e.g., dilutions of plasma from CD19-immunized mice) are added to each well and incubated for 1-2 hours at 37 0 C. The plates are washed with PBS/Tween and then incubated with secondary reagent (e.g., for human antibodies, a goat-anti-human IgG Fc- specific polyclonal reagent) conjugated to alkaline phosphatase for 1 hour at 37°C. After washing, the plates are developed with pNPP substrate (1 mg/ml), and analyzed at OD of 405-650. Preferably, mice which develop the highest titers will be used for fusions.
  • secondary reagent e.g., for human antibodies
  • An ELISA assay as described above can also be used to screen for hybridomas that show positive reactivity with CD 19 immunogen.
  • Hybridomas that bind with high avidity and/or affinity to a CD 19 protein are subcloned and further characterized.
  • One clone from each hybridoma, which retains the reactivity of the parent cells (by ELISA) can be chosen for making a 5-10 vial cell bank stored at -140 0 C, and for antibody purification.
  • selected hybridomas can be grown in two-liter spinner-flasks for monoclonal antibody purification.
  • Supernatants can be filtered and concentrated before affinity chromatography with protein A-sepharose (Pharmacia, Piscataway, NJ).
  • Eluted IgG can be checked by gel electrophoresis and high performance liquid chromatography to ensure purity.
  • the buffer solution can be exchanged into PBS, and the concentration can be determined by OD280 using 1.43 extinction coefficient.
  • the monoclonal antibodies can be aliquoted and stored at -80 0 C. To determine if the selected anti-CD 19 monoclonal antibodies bind to unique epitopes, each antibody can be biotinylated using commercially available reagents
  • isotype ELISAs can be performed using reagents specific for antibodies of a particular isotype. For example, to determine the isotype of a human monoclonal antibody, wells of microtiter plates can be coated with 1 ⁇ g/ml of anti-human immunoglobulin overnight at 4°C.
  • the plates After blocking with 1% BSA, the plates are reacted with 1 ⁇ g /ml or less of test monoclonal antibodies or purified isotype controls, at ambient temperature for one to two hours. The wells can then be reacted with either human IgGl or human IgM-specific alkaline phosphatase-conjugated probes. Plates are developed and analyzed as described above.
  • Anti-CD 19 human IgGs can be further tested for reactivity with CD 19 antigen by Western blotting. Briefly, CD 19 can be prepared and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. After electrophoresis, the separated antigens are transferred to nitrocellulose membranes, blocked with 10% fetal calf serum, and probed with the monoclonal antibodies to be tested. Human IgG binding can be detected using anti-human IgG alkaline phosphatase and developed with BCIP/NBT substrate tablets (Sigma Chem. Co., St. Louis, Mo.).
  • the binding specificity of an antibody of this disclosure may also be determined by monitoring binding of the antibody to cells expressing a CD 19 protein, for example by flow cytometry.
  • Cells or cell lines that naturally expresses CD 19 protein such OVCAR3, NCI-H226, CFPAC-I or KB cells (described further in Example 3) may be used or a cell line, such as a CHO cell line, may be transfected with an expression vector encoding CD 19 such that CD 19 is expressed on the surface of the cells.
  • the transfected protein may comprise a tag, such as a myc-tag or a his-tag, preferably at the N-terminus, for detection using an antibody to the tag.
  • Binding of an antibody of this disclosure to a CD 19 protein may be determined by incubating the transfected cells with the antibody, and detecting bound antibody. Binding of an antibody to the tag on the transfected protein may be used as a positive control.
  • the present disclosure features bispecific molecules comprising an anti-CD 19 antibody, or a fragment thereof, of this disclosure.
  • An antibody of this disclosure, or antigen-binding portions thereof can be derivatized or linked to another functional molecule, e.g., another peptide or protein (e.g., another antibody or ligand for a receptor) to generate a bispecific molecule that binds to at least two different binding sites or target molecules.
  • the antibody of this disclosure may in fact be derivatized or linked to more than one other functional molecule to generate multispecific molecules that bind to more than two different binding sites and/or target molecules; such multispecific molecules are also intended to be encompassed by the term "bispecific molecule" as used herein.
  • an antibody of this disclosure can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovalent association or otherwise) to one or more other binding molecules, such as another antibody, antibody fragment, peptide or binding mimetic, such that a bispecific molecule results.
  • the present disclosure includes bispecific molecules comprising at least one first binding specificity for CD 19 and a second binding specificity for a second target epitope.
  • the second target epitope is an Fc receptor, e.g., human Fc ⁇ RI (CD64) or a human Fc ⁇ receptor (CD89). Therefore, this disclosure includes bispecific molecules capable of binding both to Fc ⁇ R or Fc ⁇ R expressing effector cells (e.g., monocytes, macrophages or polymorphonuclear cells
  • the molecule can further include a third binding specificity, in addition to an anti-Fc binding specificity and an anti-CD 19 binding specificity.
  • the third binding specificity is an anti-enhancement factor (EF) portion, e.g..
  • the "anti-enhancement factor portion” can be an antibody, functional antibody fragment or a ligand that binds to a given molecule, e.g., an antigen or a receptor, and thereby results in an enhancement of the effect of the binding determinants for the Fc receptor or target cell antigen.
  • the "anti-enhancement factor portion” can bind an Fc receptor or a target cell antigen.
  • the anti- enhancement factor portion can bind to an entity that is different from the entity to which the first and second binding specificities bind.
  • the anti-enhancement factor portion can bind a cytotoxic T-cell (e.g., via CD2, CD3, CD8, CD28, CD4, CD40, ICAM-I or other immune cell that results in an increased immune response against the target cell).
  • the bispecific molecules of this disclosure comprise as a binding specificity at least one antibody, or an antibody fragment thereof, including, e.g., an Fab, Fab', F(ab') 2 , Fv, Fd, dAb or a single chain Fv.
  • the antibody may also be a light chain or heavy chain dimer, or any minimal fragment thereof such as a Fv or a single chain construct as described in U.S. Patent No.
  • the binding specificity for an Fc ⁇ receptor is provided by a monoclonal antibody, the binding of which is not blocked by human immunoglobulin G (IgG).
  • IgG receptor refers to any of the eight ⁇ -chain genes located on chromosome 1. These genes encode a total of twelve transmembrane or soluble receptor isoforms which are grouped into three Fc ⁇ receptor classes: Fc ⁇ RI (CD64), Fc ⁇ RII(CD32), and Fc ⁇ RIII (CD 16).
  • the Fc ⁇ receptor a human high affinity Fc ⁇ RI.
  • the human Fc ⁇ RI is a 72 kDa molecule, which shows high affinity for monomeric IgG (10 b - 10 ⁇ M "1 ).
  • the hybridoma producing mAb 32 is available from the American Type Culture Collection, ATCC Accession No. HB9469.
  • the anti-Fc ⁇ receptor antibody is a humanized form of monoclonal antibody 22 (H22).
  • H22 monoclonal antibody 22
  • the production and characterization of the H22 antibody is described in Graziano, R. F. et al. (1995) J. Immunol 155 (10): 4996-5002 and PCT Publication WO 94/10332 to Tempest et al..
  • the H22 antibody producing cell line was deposited at the American Type Culture Collection under the designation HA022CL1 and has the accession no. CRL 1 1 177.
  • the binding specificity for an Fc receptor is provided by an antibody that binds to a human IgA receptor, e.g., an Fc-alpha receptor (Fc ⁇ RI (CD89)), the binding of which is preferably not blocked by human immunoglobulin A (IgA).
  • IgA receptor is intended to include the gene product of one ⁇ -gene (Fc ⁇ RI) located on chromosome 19. This gene is known to encode several alternatively spliced transmembrane isoforms of 55 to 1 10 kDa.
  • Fc ⁇ RI (CD89) is constitutively expressed on monocytes/macrophages, eosinophilic and neutrophilic granulocytes, but not on non-effector cell populations.
  • Fc ⁇ RI has medium affinity (« 5 ⁇ 10 7 M "1 ) for both IgAl and IgA2, which is increased upon exposure to cytokines such as G-CSF or GM-CSF (Morton, H.C. et al. ( 1996) Critical Reviews in Immunology J_6:423-440).
  • cytokines such as G-CSF or GM-CSF
  • Fc ⁇ RI and Fc ⁇ RI are preferred trigger receptors for use in the bispecific molecules of this disclosure because they are ( 1 ) expressed primarily on immune effector cells, e.g., monocytes, PMNs, macrophages and dendritic cells; (2) expressed at high levels (e.g., 5,000-100,000 per cell); (3) mediators of cytotoxic activities (e.g., ADCC, phagocytosis); and (4) mediate enhanced antigen presentation of antigens, including self- antigens, targeted to them. While human monoclonal antibodies are preferred, other antibodies which can be employed in the bispecific molecules of this disclosure are murine, chimeric and humanized monoclonal antibodies.
  • the bispecific molecules of the present disclosure can be prepared by conjugating the constituent binding specificities, e.g., the anti-FcR and anti-CD 19 binding specificities, using methods known in the art. For example, each binding specificity of the bispecific molecule can be generated separately and then conjugated to one another. When the binding specificities are proteins or peptides, a variety of coupling or cross- linking agents can be used for covalent conjugation.
  • cross-linking agents examples include protein A, carbodiimide, N-succinimidyl-S-acetyl-thioacetate (SATA), 5,5'- dithiobis(2-nitrobenzoic acid) (DTNB), o-phenylenedimaleimide (oPDM), N- succinimidyl-3-(2-pyridyldithio)propionate (SPDP), and sulfosuccinimidyl 4-(N- maleimidomethyl) cyclohaxane-1-carboxylate (sulfo-SMCC) (see e.g., Karpovsky et al. (1984) J. Exp. Med.
  • Preferred conjugating agents are SATA and sulfo-SMCC, both available from Pierce Chemical Co. (Rockford, IL).
  • the binding specificities are antibodies, they can be conjugated via sulfhydryl bonding of the C-terminus hinge regions of the two heavy chains.
  • the hinge region is modified to contain an odd number of sulfhydryl residues, preferably one, prior to conjugation.
  • both binding specificities can be encoded in the same vector and expressed and assembled in the same host cell.
  • This method is particularly useful where the bispecific molecule is a mAb x mAb, mAb x Fab, Fab x F(ab') 2 or ligand x Fab fusion protein.
  • a bispecific molecule of this disclosure can be a single chain molecule comprising one single chain antibody and a binding determinant, or a single chain bispecific molecule comprising two binding determinants. Bispecific molecules may comprise at least two single chain molecules. Methods for preparing bispecific molecules are described for example in U.S.
  • Binding of the bispecific molecules to their specific targets can be confirmed by, for example, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), FACS analysis, bioassay (e.g., growth inhibition), or Western Blot assay.
  • ELISA enzyme-linked immunosorbent assay
  • RIA radioimmunoassay
  • FACS fluorescence-activated cell sorting
  • bioassay e.g., growth inhibition
  • Western Blot assay Western Blot assay.
  • Each of these assays generally detects the presence of protein-antibody complexes of particular interest by employing a labeled reagent (e.g., an antibody) specific for the complex of interest.
  • a labeled reagent e.g., an antibody
  • the FcR-antibody complexes can be detected using e.g., an enzyme-linked antibody or antibody fragment which recognizes and specifically binds to the antibody- FcR complexes.
  • the antibody can be radioactively labeled and used in a radioimmunoassay (RIA) (see, for example, Weintraub, B., Principles of Radioimmunoassays, Seventh Training Course on Radioligand Assay Techniques, The Endocrine Society, March, 1986, which is incorporated by reference herein).
  • RIA radioimmunoassay
  • the radioactive isotope can be detected by such means as the use of aycounter or a scintillation counter or by autoradiography.
  • the present invention provides for antibody-partner conjugates where the antibody is linked to the partner through a chemical linker.
  • the linker is a peptidyl linker, and is depicted herein as (L 4 ) p — F — (L 1 ) m .
  • Other linkers include hydrazine and disulfide linkers, and is depicted herein as (L 4 ) p — H — (L 1 ) m or (L 4 ) p — J— (L 1 ) m , respectively.
  • the present invention also provides cleavable linker arms that are appropriate for attachment to essentially any molecular species.
  • linker arm aspect of the invention is exemplified herein by reference to their attachment to a therapeutic moiety. It will, however, be readily apparent to those of skill in the art that the linkers can be attached to diverse species including, but not limited to, diagnostic agents, analytical agents, biomolecules, targeting agents, detectable labels and the like.
  • the present invention relates to linkers that are useful to attach targeting groups to therapeutic agents and markers.
  • the invention provides linkers that impart stability to compounds, reduce their in vivo toxicity, or otherwise favorably affect their pharmacokinetics, bioavailability and/or pharmacodynamics. It is generally preferred that in such embodiments, the linker is cleaved, releasing the active drug, once the drug is delivered to its site of action.
  • the linkers of the invention are traceless, such that once removed from the therapeutic agent or marker (such as during activation), no trace of the linker's presence remains.
  • the linkers are characterized by their ability to be cleaved at a site in or near the target cell such as at the site of therapeutic action or marker activity. Such cleavage can be enzymatic in nature. This feature aids in reducing systemic activation of the therapeutic agent or marker, reducing toxicity and systemic side effects. Preferred cleavable groups for enzymatic cleavage include peptide bonds, ester linkages, and disulfide linkages.
  • the linkers are sensitive to pH and are cleaved through changes in pH. An important aspect of the current invention is the ability to control the speed with which the linkers cleave. Often a linker that cleaves quickly is desired.
  • a linker that cleaves more slowly may be preferred.
  • WO 02/096910 provides several specific ligand-drug complexes having a hydrazine linker.
  • the linker composition dependent upon the rate of cyclization required, and the particular compounds described cleave the ligand from the drug at a slower rate than is preferred for many drug-linker conjugates.
  • the hydrazine linkers of the current invention provide for a range of cyclization rates, from very fast to very slow, thereby allowing for the selection of a particular hydrazine linker based on the desired rate of cyclization.
  • very fast cyclization can be achieved with hydrazine linkers that produce a single 5-membered ring upon cleavage.
  • Preferred cyclization rates for targeted delivery of a cytotoxic agent to cells are achieved using hydrazine linkers that produce, upon cleavage, either two 5-membered rings or a single 6-membered ring resulting from a linker having two methyls at the geminal position.
  • the gx-w-dimethyl effect has been shown to accelerate the rate of the cyclization reaction as compared to a single 6- membered ring without the two methyls at the geminal position. This results from the strain being relieved in the ring.
  • substitutents may slow down the reaction instead of making it faster. Often the reasons for the retardation can be traced to steric hindrance.
  • the gem dimethyl substitution allows for a much faster cyclization reaction to occur compared to when the geminal carbon is a CHi.
  • a linker that cleaves more slowly may be preferred.
  • a linker which cleaves more slowly may be useful.
  • a slow rate of cyclization is achieved using a hydrazine linker that produces, upon cleavage, either a single 6-membered ring, without the gem-dimethyl substitution, or a single 7-membered ring.
  • the linkers also serve to stabilize the therapeutic agent or marker against degradation while in circulation. This feature provides a significant benefit since such stabilization results in prolonging the circulation half-life of the attached agent or marker.
  • the linker also serves to attenuate the activity of the attached agent or marker so that the conjugate is relatively benign while in circulation and has the desired effect, for example is toxic, after activation at the desired site of action.
  • this feature of the linker serves to improve the therapeutic index of the agent.
  • the stabilizing groups are preferably selected to limit clearance and metabolism of the therapeutic agent or marker by enzymes that may be present in blood or non-target tissue and are further selected to limit transport of the agent or marker into the cells.
  • the stabilizing groups serve to block degradation of the agent or marker and may also act in providing other physical characteristics of the agent or marker.
  • the stabilizing group may also improve the agent or marker's stability during storage in either a formulated or non-formulated form.
  • the stabilizing group is useful to stabilize a therapeutic agent or marker if it serves to protect the agent or marker from degradation when tested by storage of the agent or marker in human blood at 37°C for 2 hours and results in less than 20%, preferably less than 10%, more preferably less than 5% and even more preferably less than 2%, cleavage of the agent or marker by the enzymes present in the human blood under the given assay conditions.
  • the present invention also relates to conjugates containing these linkers. More particularly, the invention relates to prodrugs that may be used for the treatment of disease, especially for cancer chemotherapy. Specifically, use of the linkers described herein provide for prodrugs that display a high specificity of action, a reduced toxicity, and an improved stability in blood relative to prodrugs of similar structure.
  • linkers of the present invention as described herein may be present at a variety of positions within the partner molecule.
  • linker that may contain any of a variety of groups as part of its chain that will cleave in vivo, e.g., in the blood stream, at a rate which is enhanced relative to that of constructs that lack such groups.
  • conjugates of the linker amis with therapeutic and diagnostic agents are useful to form prodrug analogs of therapeutic agents and to reversibly link a therapeutic or diagnostic agent to a targeting agent, a detectable label, or a solid support.
  • the linkers may be incorporated into complexes that include a cytotoxin.
  • Activation of a prodrug may be achieved by an esterase, both within tumor cells and in several normal tissues, including plasma.
  • the level of relevant esterase activity in humans has been shown to be very similar to that observed in rats and non-human primates, although less than that observed in mice.
  • Activation of a prodrug may also be achieved by cleavage by glucuronidase.
  • one or more self-immolative linker groups L 1 are optionally introduced between the cytotoxin and the targeting agent.
  • linker groups may also be described as spacer groups and contain at least two reactive functional groups.
  • one chemical functionality of the spacer group bonds to a chemical functionality of the therapeutic agent, e.g., cytotoxin, while the other chemical functionality of the spacer group is used to bond to a chemical functionality of the targeting agent or the cleavable linker.
  • Examples of chemical functionalities of spacer groups include hydroxy, mercapto, carbonyl, carboxy, amino, ketone, and mercapto groups.
  • the self-immolative linkers represented by L 1 , are generally a substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl or substituted or unsubstituted heteroalkyl group.
  • the alkyl or aryl groups may comprise between 1 and 20 carbon atoms. They may also comprise a polyethylene glycol moiety.
  • Exemplary spacer groups include, for example, 6-aminohexanol, 6- mercaptohexanol, 10-hydroxydecanoic acid, glycine and other amino acids, 1 ,6- hexanediol, ⁇ -alanine, 2-aminoethanol, cysteamine (2-aminoethanethiol), 5- aminopentanoic acid, 6-aminohexanoic acid, 3-maleimidobenzoic acid, phthalide, ⁇ - substituted phthalides, the carbonyl group, aminal esters, nucleic acids, peptides and the like.
  • the spacer can serve to introduce additional molecular mass and chemical functionality into the cytotoxin-targeting agent complex. Generally, the additional mass and functionality will affect the serum half-life and other properties of the complex. Thus, through careful selection of spacer groups, cytotoxin complexes with a range of serum half-lives can be produced.
  • the spacer(s) located directly adjacent to the drug moiety is also denoted as (L 1 ),,,, wherein m is an integer selected from 0, 1, 2, 3, 4, 5, and 6.
  • L 1 may be any self- immolative group.
  • L 4 is a linker moiety that preferably imparts increased solubility or decreased aggregation properties to conjugates utilizing a linker that contains the moiety or modifies the hydrolysis rate of the conjugate.
  • the L 4 linker does not have to be self immolative.
  • the L 4 moiety is substituted alkyl, unsubstituted alkyl, substituted aryl, unsubstituted aryl, substituted heteroalkyl, or unsubstituted heteroalkyl, any of which may be straight, branched, or cyclic.
  • the substitutions may be, for example, a lower (C '-C 6 ) alkyl, alkoxy, aklylthio, alkylamino, or dialkylamino.
  • L comprises a non-cyclic moiety.
  • L comprises any positively or negatively charged amino acid polymer, such as polylysine or polyargenine.
  • L 4 can comprise a polymer such as a polyethylene glycol moiety.
  • the L 4 linker can comprise, for example, both a polymer component and a small chemical moiety.
  • L 4 comprises a polyethylene glycol (PEG) moiety.
  • the PEG portion of L 4 may be between 1 and 50 units long.
  • the PEG will have 1-12 repeat units, more preferably 3-12 repeat units, more preferably 2-6 repeat units, or even more preferably 3-5 repeat units and most preferably 4 repeat units.
  • L 4 may consist solely of the PEG moiety, or it may also contain an additional substituted or unsubstituted alkyl or heteroalkyl. It is useful to combine PEG as part of the L 4 moiety to enhance the water solubility of the complex. Additionally, the PEG moiety reduces the degree of aggregation that may occur during the conjugation of the drug to the antibody.
  • L comprises
  • R 20 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl.
  • Each R 25 , R 25 , R 26 , and R 26 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl; and s and t are independently integers from 1 to 6.
  • R " °.
  • R 2 ⁇ R 2 * , R 26 and R 26 are hydrophobic.
  • R 20 is H or alkyl (preferably, unsubstituted lower alkyl).
  • R 25 , R 25 , R 26 and R 26 are independently H or alkyl (preferably, unsubstituted C 1 to C 4 alkyl).
  • R 23 , R 23 , R 26 and R 26 are all H.
  • t is 1 and s is 1 or 2.
  • the peptidyl linkers of the invention can be represented by the general formula: (L 4 ) p — F — (L 1 ) m , wherein F represents the linker portion comprising the peptidyl moiety.
  • the F portion comprises an optional additional self-immolative linker(s), L 2 , and a carbonyl group.
  • the F portion comprises an amino group and an optional spacer group(s), L 3 .
  • the conjugate comprising the peptidyl linker comprises a structure of the following formula (a):
  • L 1 is a self-immolative linker, as described above, and L 4 is a moiety that preferably imparts increased solubility, or decreased aggregation properties, or modifies the hydrolysis rate, as described above.
  • L 2 represents a self-immolative linker(s).
  • m is 0, 1, 2, 3, 4, 5, or 6; and o and p are independently 0 or 1.
  • AA 1 represents one or more natural amino acids, and/or unnatural ⁇ -amino acids; c is an integer from 1 and 20. In some embodiments, c is in the range of 2 to 5 or c is 2 or 3.
  • AA 1 is linked, at its amino terminus, either directly to L 4 or, when L 4 is absent, directly to the X 4 group (i.e., the targeting agent, detectable label, protected reactive functional group or unprotected reactive functional group).
  • L 4 when L is present, L 4 does not comprise a carboxylic acyl group directly attached to the N-terminus of (AA ') c .
  • there it is not necessary in these embodiments for there to be a carboxylic acyl unit directly between either L 4 or X 4 and AA 1 , as is necessary in the peptidic linkers of U.S. Patent No. 6,214,345.
  • the conjugate comprising the peptidyl linker comprises a structure of the following formula (b):
  • L 4 is a moiety that preferably imparts increased solubility, or decreased aggregation properties, or modifies the hydrolysis rate, as described above;
  • L 3 is a spacer group comprising a primary or secondary amine or a carboxyl functional group, and either the amine of L 3 forms an amide bond with a pendant carboxyl functional group of D or the carboxyl of L 3 forms an amide bond with a pendant amine functional group of D; and o and p are independently 0 or 1.
  • AA 1 represents one or more natural amino acids, and/or unnatural ⁇ -amino acids;
  • c is an integer from 1 and 20.
  • L is absent (i.e., m is 0 in the general formula).
  • AA is linked, at its amino terminus, either directly to L 4 or, when L 4 is absent, directly to the X 4 group (i.e., the targeting agent, detectable label, protected reactive functional group or unprotected reactive functional group).
  • L 4 when L 4 is present, L 4 does not comprise a carboxylic acyl group directly attached to the N-terminus of (AA ') c .
  • carboxylic acyl unit directly between either L 4 or X 4 and AA 1 , as is necessary in the peptidic linkers of U.S. Patent No. 6,214,345.
  • the self-immolative linker L 2 is a bifunctional chemical moiety which is capable of covalently linking together two spaced chemical moieties into a normally stable tripartate molecule, releasing one of said spaced chemical moieties from the tripartate molecule by means of enzymatic cleavage; and following said enzymatic cleavage, spontaneously cleaving from the remainder of the molecule to release the other of said spaced chemical moieties.
  • the self-immolative spacer is covalently linked at one of its ends to the peptide moiety and covalently linked at its other end to the chemically reactive site of the drug moiety whose derivatization inhibits pharmacological activity, so as to space and covalently link together the peptide moiety and the drug moiety into a tripartate molecule which is stable and pharmacologically inactive in the absence of the target enzyme, but which is enzymatically cleavable by such target enzyme at the bond covalently linking the spacer moiety and the peptide moiety to thereby affect release of the peptide moiety from the tripartate molecule.
  • Such enzymatic cleavage will activate the self-immolating character of the spacer moiety and initiate spontaneous cleavage of the bond covalently linking the spacer moiety to the drug moiety, to thereby affect release of the drug in pharmacologically active form.
  • the self-immolative linker L 2 may be any self-immolative group.
  • L 2 is a substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, unsubstituted heterocycloalkyl, substituted heterocycloalkyl, substituted and unsubstituted aryl, and substituted and unsubstituted heteroaryl.
  • One particularly preferred seif-immolative spacer L 2 may be represented by the formula (c):
  • the aromatic ring of the aminobenzyl group may be substituted with one or more "K" groups.
  • a “K” group is a substituent on the aromatic ring that replaces a hydrogen otherwise attached to one of the four non-substituted carbons that are part of the ring structure.
  • the "K” group may be a single atom, such as a halogen, or may be a multi- atom group, such as alkyl, heteroalkyl, amino, nitro, hydroxy, alkoxy, haloalkyl, and cyano.
  • Each K is independently selected from the group consisting of substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO 2 , NR 21 R 22 , NR 21 COR 22 , OCONR 21 R 22 , OCOR 21 , and OR 21 , wherein R 21 and R 22 are independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl and unsubstituted heterocycloalkyl.
  • K substituents include, but are not limited to, F, Cl, Br, I, NO 2 , OH, OCH 3 , NHCOCH 3 , N(CHj) 2 , NHCOCF 3 and methyl.
  • K 1 is an integer of O, 1 , 2, 3, or 4. In one preferred embodiment, / is O.
  • the ether oxygen atom of the structure shown above is connected to a carbonyl group.
  • the line from the NR 24 functionality into the aromatic ring indicates that the amine functionality may be bonded to any of the five carbons that both form the ring and are not substituted by the -CH 2 -O- group.
  • the NR 24 functionality of X is covalently bound to the aromatic ring at the para position relative to the -CH 2 -O- group.
  • R 24 is a member selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl. In a specific embodiment, R 24 is hydrogen.
  • the invention provides a peptide linker of formula (a) above, wherein F comprises the structure:
  • R 24 is selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl.
  • Each K is a member independently selected from the group consisting of substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO 2 , NR 21 R 22 , NR 21 COR 22 , OCONR 21 R 22 , OCOR 21 , and OR 21 where R 21 and R 22 are independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted
  • the peptide linker of formula (a) above comprises a -F- (L 1 ),,,- that comprises the structure:
  • each R » 2"4 i • s a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl.
  • the self-immolative spacer L 1 or L " includes
  • each R 17 , R 18 , and R 19 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryl, and w is an integer from 0 to 4.
  • R 17 and R 18 are independently H or alkyl (preferably, unsubstituted C 1-4 alkyl).
  • R 17 and R are Cl -4 alkyl, such as methyl or ethyl.
  • w is 0. While not wishing to be bound to any particular theory, it has been found experimentally that this particular self-immolative spacer cyclizes relatively quickly.
  • L 1 or L 2 includes
  • the spacer group L J is characterized in that it comprises a primary or secondary amine or a carboxyl functional group, and either the amine of the L group forms an amide bond with a pendant carboxyl functional group of D or the carboxyl of L J forms an amide bond with a pendant amine functional group of D.
  • L 3 can be selected from the group consisting of substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted hteroaryl, or substituted or unsubstituted heterocycloalkyl.
  • L 3 comprises an aromatic group. More preferably, L 3 comprises a benzoic acid group, an aniline group or indole group.
  • Non-limiting examples of structures that can serve as an -L 3 -NH- spacer include the following structures:
  • Z is a member selected from O, S and NR 23
  • R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl.
  • the IS moiety Upon cleavage of the linker of the invention containing L 3 , the IS moiety remains attached to the drug, D. Accordingly, the L/ moiety is chosen such that its presence attached to D does not significantly alter the activity of D.
  • a portion of the drug D itself functions as the L 3 spacer.
  • the drug, D is a duocarmycin derivative in which a portion of the drug functions as the L spacer
  • Non-hmiting examples of such embodiments include those in which NHo- (L 3 )-D has a structure selected fiom the group consisting of
  • Z is a member selected fiom O, S and NR 2 ⁇ where R 2 ⁇ is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl; and where the NH 2 group on each structure reacts with (AA ') c to form -(AA') C -NH-.
  • the Peptide Sequence AA represents a single amino acid or a plurality of ammo acids that are joined together by amide bonds.
  • the amino acids may be natural amino acids and/or unnatui al ⁇ -amino acids
  • the peptide of the current invention is selected for directing enzyme-catalyzed cleavage of the peptide by an enzyme in a location of interest in a biological system.
  • a peptide is chosen that is cleaved by one or more proteases that may exist in the extracellular matrix, e.g., due to release of the cellular contents of nearby dying cells, such that the peptide is cleaved extracellularly.
  • the number of amino acids within the peptide can range from 1 to 20; but more preferably there will be 1-8 amino acids, 1-6 amino acids or 1, 2, 3 or 4 amino acids comprising (AA ') c .
  • Peptide sequences that are susceptible to cleavage by specific enzymes or classes of enzymes are well known in the art. Many peptide sequences that are cleaved by enzymes in the serum, liver, gut, etc. are known in the art.
  • An exemplary peptide sequence of the invention includes a peptide sequence that is cleaved by a protease. The focus of the discussion that follows on the use of a protease-sensitive sequence is for clarity of illustration and does not serve to limit the scope of the present invention.
  • the linker When the enzyme that cleaves the peptide is a protease, the linker generally includes a peptide containing a cleavage recognition sequence for the protease.
  • a cleavage recognition sequence for a protease is a specific amino acid sequence recognized by the protease during proteolytic cleavage.
  • Many protease cleavage sites are known in the art, and these and other cleavage sites can be included in the linker moiety. See, e.g., Matayoshi et al. Science 2Al ⁇ 954 ( 1990); Dunn et al. Meth. Enzymol. 241 : 254 (1994); Seidah et al. Meth. Enzymol.
  • the amino acids of the peptide sequence (AA') C are chosen based on their suitability for selective enzymatic cleavage by particular molecules such as tumor- associated protease.
  • the amino acids used may be natural or unnatural amino acids. They may be in the L or the D configuration. In one embodiment, at least three different amino acids are used. In another embodiment, only two amino acids are used.
  • the peptide sequence (AA') C is chosen based on its ability to be cleaved by a lysosomal proteases, non-limiting examples of which include cathepsins B, C, D, H, L and S.
  • the peptide sequence (AA 1 ) c is capable of being cleaved by cathepsin B in vitro, which can be tested using in vitro protease cleavage assays known in the art.
  • the peptide sequence (AA ') c is chosen based on its ability to be cleaved by a tumor-associated protease, such as a protease that is found extracellularly in the vicinity of tumor cells, non-limiting examples of which include thimet oligopeptidase (TOP) and CD 10.
  • TOP thimet oligopeptidase
  • the ability of a peptide to be cleaved by TOP or CD 10 can be tested using in vitro protease cleavage assays known in the art.
  • Suitable, but non-limiting, examples of peptide sequences suitable for use in the conjugates of the invention include Val-Cit, Cit-Cit, Val-Lys, Phe-Lys, Lys-Lys, AIa- Lys, Phe-Cit, Leu-Cit, Ile-Cit, Tip, Gt, Phe-Ala, Phe-N 9 -tosyl-Arg, Phe-N 9 -nitro-Arg, Phe-Phe-Lys, D-Phe-Phe-Lys, Gly-Phe-Lys, Leu-Ala-Leu, Ile-Ala-Leu, Val-Ala-Val, Ala-Leu- Ala-Leu (SEQ ID NO:88), ⁇ -Ala-Leu- Ala-Leu (SEQ ID NO:89), Gly-Phe-Leu- GIy (SEQ ID NO:90), Val-Ala, Leu-Leu-Gly-Leu (SEQ
  • the amino acid located the closest to the drug moiety is selected from the group consisting of: Ala, Asn, Asp, Cit, Cys, GIn, GIu, GIy, He, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and VaL
  • the amino acid located the closest to the drug moiety is selected from the group consisting of: Ala, Asn, Asp, Cys, GIn, GIu, GIy, He, Leu, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and VaL
  • Proteases have been implicated in cancer metastasis. Increased synthesis of the protease urokinase was correlated with an increased ability to metastasize in many cancers.
  • Urokinase activates plasmin from plasminogen, which is ubiquitously located in the extracellular space and its activation can cause the degradation of the proteins in the extracellular matrix through which the metastasizing tumor cells invade. Plasmin can also activate the collagenases thus promoting the degradation of the collagen in the basement membrane surrounding the capillaries and lymph system thereby allowing tumor cells to invade into the target tissues (Dano, et al. Adv. Cancer. Res., 44: 139 (1985)).
  • the invention also provides the use of peptide sequences that are sensitive to cleavage by tryptases.
  • Human mast cells express at least four distinct tryptases. designated ⁇ ⁇ l, ⁇ lf, and ⁇ lll. These enzymes are not controlled by blood plasma proteinase inhibitors and only cleave a few physiological substrates in vitro.
  • the tryptase family of serine proteases has been implicated in a variety of allergic and inflammatory diseases involving mast cells because of elevated tryptase levels found in biological fluids from patients with these disorders. However, the exact role of tryptase in the pathophysiology of disease remains to be delineated. The scope of biological functions and coi ⁇ esponding physiological consequences of tryptase are substantially defined by their substrate specificity.
  • Tryptase is a potent activator of pro-urokinase plasminogen activator (uPA) the zymogen form of a protease associated with tumor metastasis and invasion. Activation of the plasminogen cascade, resulting in the destruction of extracellular matrix for cellular extravasation and migration, may be a function of tryptase activation of pro-urokinase plasminogen activator at the P4-P1 sequence of Pro-Arg-Phe-Lys (SEQ ID NO:91) (Stack, et al., Journal of Biological Chemistry 269 (13): 9416-9419 (1994)).
  • uPA pro-urokinase plasminogen activator
  • Vasoactive intestinal peptide a neuropeptide that is implicated in the regulation of vascular permeability, is also cleaved by tryptase, primarily at the Thr-Arg-Leu-Arg (SEQ ID NO:92) sequence (Tarn, et al., Am. J. Respir. Cell MoL Biol. 3: 27-32 (1990)).
  • the G- protein coupled receptor PAR-2 can be cleaved and activated by tryptase at the Ser-Lys- GIy- Arg (SEQ ID NO:93) sequence to drive fibroblast proliferation, whereas the thrombin activated receptor PAR-I is inactivated by tryptase at the Pro-Asn-Asp-Lys (SEQ ID NO:94) sequence (Molino et al., Journal of Biological Chemistry 272(7): 4043- 4049 (1997)).
  • SEQ ID NO:94 Pro-Asn-Asp-Lys
  • the antibody-partner conjugate of the current invention may optionally contain two or more linkers. These linkers may be the same or different. For example, a peptidyl linker may be used to connect the drug to the ligand and a second peptidyl linker may attach a diagnostic agent to the complex. Other uses for additional linkers include linking analytical agents, biomolecules, targeting agents, and detectable labels to the antibody- partner complex.
  • compounds of the invention that are poly- or multi-valent species, including, for example, species such as dimers, trimers, tetramers and higher homologs of the compounds of the invention or reactive analogues thereof.
  • the poly- and multi-valent species can be assembled from a single species or more than one species of the invention.
  • a dimeric construct can be "homo-dimeric" or 'lieterodimeric.' ' '
  • poly- and multi-valent constructs in which a compound of the invention or a reactive analogue thereof, is attached to an oligomeric or polymeric framework are within the scope of the present invention.
  • the framework is preferably polyfunctional (i.e. having an array of reactive sites for attaching compounds of the invention).
  • the framework can be derivatized with a single species of the invention or more than one species of the invention.
  • the present invention includes compounds that are functionalized to afford compounds having water-solubility that is enhanced relative to analogous compounds that are not similarly functionalized.
  • any of the substituents set forth herein can be replaced with analogous radicals that have enhanced water solubility.
  • additional water solubility is imparted by substitution at a site not essential for the activity towards the ion channel of the compounds set forth herein with a moiety that enhances the water solubility of the parent compounds.
  • Such methods include, but are not limited to, functionalizing an organic nucleus with a permanently charged moiety, e.g., quaternary ammonium, or a group that is charged at a physiologically relevant pH, e.g. carboxylic acid, amine.
  • Other methods include, appending to the organic nucleus hydroxyl- or amine-containing groups, e.g. alcohols, polyols, polyethers, and the like.
  • Representative examples include, but are not limited to, polylysine, polyethyleneimine, poly(ethyleneglycol) and poly(propyleneglycol). Suitable functionalization chemistries and strategies for these compounds are known in the art.
  • the conjugate of the invention comprises a hydrazine self-immolative linker, wherein the conjugate has the structure:
  • nj is an integer from 1 - 10; n 2 is 0, 1, or 2; each R 24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl; and I is either a bond (i.e., the bond between the carbon of the backbone and the adjacent nitrogen) or:
  • n 3 is 0 or 1 , with the proviso that when n ⁇ is 0, n 2 is not 0; and 11 4 is I, 2, or 3, wherein when I is a bond, ni is 3 and n 2 is 1 , D can not be
  • R is Me or CH 2 - CH 2 -NMe 2 .
  • the substitution on the phenyl ring is a para substitution.
  • n> is 2, 3, or 4 or ni is 3.
  • n 2 is 1.
  • I is a bond (i.e., the bond between the carbon of the backbone and the adjacent nitrogen).
  • the hydrazine linker, H can form a 6- membered self immolative linker upon cleavage, for example, when n 3 is 0 and 11 4 is 2.
  • the hydrazine linker, H can form two 5-membered self immolative linkers upon cleavage.
  • H forms a 5-membered self immolative linker
  • H forms a 7-membered self immolative linker
  • H forms a 5-membered self immolative linker and a 6-membered self immolative linker, upon cleavage.
  • the rate of cleavage is affected by the size of the ring formed upon cleavage. Thus, depending upon the rate of cleavage desired, an appropriate size ring to be formed upon cleavage can be selected.
  • the hydrazine linker comprises a 5-membered hydrazine linker, wherein H comprises the structure:
  • ni is 2, 3, or 4. In another preferred embodiment, ni is 3.
  • each R 24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl.
  • each R 24 is independently H or a Ci - C 6 alkyl.
  • each R 24 is independently H or a Ci - C 3 alkyl, more preferably H or CH 3 .
  • at least one R 24 is a methyl group.
  • each R 24 is H.
  • Each R 24 is selected to tailor the compounds steric effects and for altering solubility.
  • the 5-membered hydrazine linkers can undergo one or more cyclization reactions that separate the drug from the linker, and can be described, for example, by:
  • An exemplary synthetic route for preparing a five membered linker of the invention is:
  • the Cbz-protected DMDA b is reacted with 2,2-Dimethyl-makmic acid a in solution vvitli thionyl chloride to form a Cbz-DMDA-2,2-dimethylmalonic acid c.
  • Compound c is reacted with Boc-N-methyl hydrazine d in the presence of EDC to form DMDA-2,2- dimetylmalonic-Boc-N-methylhydrazine e.
  • the hydrazine linker comprises a 6-membered hydrazine linker, wherein H comprises the structure:
  • each R 24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl.
  • each R 24 is independently H or a Ci - Ce alkyl.
  • each R 24 is independently H or a Ct - C 3 alkyl, more preferably H or CH 3 .
  • at least one R " is a methyl group.
  • each R24 is H.
  • Each R "4 is selected to tailor the compounds steric effects and for altering solubility.
  • H comprises the structure:
  • H comprises a geminal dimethyl substitution.
  • each R 24 is independently an H or a substituted or unsubstituted alkyl.
  • the 6-membered hydrazine linkers will undergo a cyclization reaction that separates the drug from the linker, and can be described as:
  • An exemplary synthetic route for preparing a six membered linker of the invention is:
  • the invention comprises a linker having seven members. This linker would likely not cyclize as quickly as the five or six membered linkers, but this may be preferred for some antibody-partner conjugates.
  • the hydrazine linker may comprise two six membered rings or a hydrazine linker having one six and one five membered cyclization products. A five and seven membered linker as well as a six and seven membered linker are also contemplated.
  • Another hydrazine structure, H has the formula:
  • each R 24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl.
  • This hydrazine structure can also form five-, six-, or seven-membered rings and additional components can be added to form multiple rings.
  • the linker comprises an enzymatically cleavable disulfide group.
  • the invention provides a cytotoxic antibody-partner compound having a structure according to Formula (d): wherein D, L 1 , L 4 , and X 4 are as defined above and described further herein, and J is a disulfide linker comprising a group having the structure:
  • the aromatic ring of the disulfides linker may be substituted with one or more "K" groups.
  • a “K” group is a substituent on the aromatic ring that replaces a hydrogen otherwise attached to one of the four non-substituted carbons that are part of the ring structure.
  • the "K” group may be a single atom, such as a halogen, or may be a multi- atom group, such as alkyl, heteroalkyl, amino, nitro, hydroxy, alkoxy, haloalkyl, and cyano.
  • K substituents independently include, but are not limited to, F, Cl, Br, I, NO 2 , OH, OCH 3 , NHCOCH 3 , N(CHj) 2 , NHCOCF . , and methyl.
  • K 1 is an integer of O, 1 , 2, 3, or 4. In a specific embodiment, / is O.
  • the linker comprises an enzymatically cleavable disulfide group of the following formula:
  • L 4 , X 4 , p, and R 24 are as described above, and d is O, 1, 2, 3, 4, 5, or 6. In a particular embodiment, d is 1 or 2.
  • d is 1 or 2.
  • d is 1 or 2.
  • the disulfides are ortho to the amine.
  • a is 0.
  • R 24 is independently selected from H and CH 3 .
  • An exemplary synthetic route for preparing a disulfide linker of the invention is as follows:
  • a solution of 3-mercaptopropionic acid a is reacted with aldrithiol-2 to form 3- methyl benzothiazolium iodide b.
  • 3-methylbenzothiazolium iodide c is reacted with sodium hydroxide to form compound d.
  • a solution of compound d with methanol is further reacted with compound b to form compound e.
  • the present invention features an antibody conjugated to a partner molecule, such as a cytotoxin, a drug (e.g., an immunosuppressant) or a radiotoxin.
  • a partner molecule such as a cytotoxin, a drug (e.g., an immunosuppressant) or a radiotoxin.
  • cytotoxin e.g., an immunosuppressant
  • radiotoxin e.g., an immunosuppressant
  • immunocytotoxins e.g., an immunosuppressant
  • a cytotoxin or cytotoxic agent includes any agent that is detrimental to (e.g., kills) cells.
  • partner molecules of the present invention include taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1 -dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof.
  • partner molecules also include, for example, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5- fluorouracil decarbazine), alkylating agents (e.g., mechlorethamine, thioepa chlorambucil, melphalan, carmustine (BSNU) and lomustine (CCNU), cyclothosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cis- dichlorodiamine platinum (II) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin), bleomycin, mithramycin, and anthramycin (AMC)), and anti-mitotic agents (e.g., vin,
  • An example of a calicheamicin antibody conjugate is commercially available (Mylotarg ⁇ ; American Home Products).
  • Preferred examples of partner molecule are CC- 1065 and the duocarmycins. CC-
  • the Upjohn Company (Pharmacia Upjohn) has also been active in preparing derivatives of CC- 1065. See, for example, U.S. Patent No. 5,739,350; 4,978,757, 5,332, 837 and 4,912,227.
  • a particularly preferred aspect of the current invention provides a cytotoxic compound having a structure according to the following formula (e):
  • ring system A is a member selected from substituted or unsubstituted aryl substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups.
  • exemplary ring systems include phenyl and pyrrole.
  • E and G are independently selected from H, substituted or unsubstituted alkyl, substituted or unsubslituted heteroalkyl, a heteroatom, a single bond or E and G are optionally joined to form a ring system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl.
  • R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl.
  • R 12 , R 13 , and R 14 independently represent H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryl, where R 12 and R 13 together with the nitrogen or carbon atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms.
  • R 12 , R l ⁇ or R 14 can include a cleavable group within its structure.
  • R 4 , R 4 ⁇ R 5 and R 5 ' are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, halogen, NO 2 , NR 15 R 16 , NC(O)R 15 , OC(O)NR 15 R 16 , OC(O)OR 15 , C(O)R 15 , SR 15 , OR 15 , CR 15 ⁇ NR 16 , and
  • n is an integer from 1 to 20, or any adjacent pair of R 4 , R 4 ', R 3 and R 5 ⁇ together with the carbon atoms to which they are attached, are joined to form a substituted or unsubstituted cycloalkyl or heterocycloalkyl ring system having from 4 to 6 members.
  • R 15 and R 16 independently represent H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl and substituted or unsubstituted peptidyl, where R 15 and R 16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms.
  • One exemplary structure is aniline.
  • R 4 , R 4 ⁇ R 5 , R 5 ⁇ R 1 ', R 12 , R 13 , R 15 and R 16 optionally contain one or more cleavable groups within their structure, such as a cleavable linker or cleavable substrate.
  • cleavable groups include, but are not limited to peptides, amino acids, hydrazines, disulfides, and cephalosporin derivatives.
  • At least one of R 4 , R 4 ', R ⁇ R 5 ⁇ R 1 1 , R 12 , R 13 , R 15 and R 16 is used to join the drug to a linker or enzyme cleavable substrate of the present invention, as described herein, for example to L 1 , if present or to F, H, J, or X", or J.
  • R 4 , R 4 ⁇ R 5 , R 5' , R 11 , R 12 , R 13 , R 15 and R 16 bears a reactive group appropriate for conjugating the compound.
  • R 4 , R 4 ', R 5 , R 5 ', R 1 ', R 12 , R 13 , R 15 and R 16 are independently selected from H, substituted alkyl and substituted heteroalkyl and have a reactive functional group at the free terminus of the alkyl or heteroalkyl moiety.
  • R 4 , R 4 ', R 5 , R 5 ', R 1 ', R 12 , R 13 , R 15 and R 16 may be conjugated to another species, e.g, targeting agent, detectable label, solid support, etc.
  • R 6 is a single bond which is either present or absent. When R 6 is present, R 6 and
  • R 7 are joined to form a cyclopropyl ring.
  • R 7 is CH 2 -X 1 or -CH 2 -.
  • X 1 represents a leaving group such as a halogen, for example Cl, Br or F.
  • the combinations of R 6 and R 7 are interpreted in a manner that does not violate the principles of chemical valence.
  • X 1 may be any leaving group.
  • Useful leaving groups include, but are not limited to, halogens, azides, sulfonic esters (e.g., alkylsulfonyl, arylsulfonyl), oxonium ions, alkyl perchlorates, ammonioalkanesulfonate esters, alkylfluorosulfonates and fluorinated compounds (e.g., triflates, nonaflates, tresylates) and the like.
  • Particular halogens useful as leaving groups are F, Cl and Br.
  • At least one of R , R '. R 3 , and R 5 ' links said drug to L , if present, or to F, H, J, or X 2 , and includes
  • v is an integer from 1 to 6; and each R 27 , R 27 , R 28 , and R 28 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl.
  • R 27 , R 27 , R 2S , and R *" are all H.
  • v is an integer from 1 to 3 (preferably, 1 ). This unit can be used to separate aryl substituents from the drug and thereby resist or avoid generating compounds that are substrates for multi-drug resistance.
  • R 1 ' includes a moiety, X 5 , that does not self-cyclize and links the drug to L 1 , if present, or to F, H, J, or X 2 .
  • the moiety, X ⁇ is preferably cleavable using an enzyme and, when cleaved, provides the active drug.
  • R can have the following structure (with the right side coupling to the remainder of the drug):
  • ring system A of fonnula (e) is a substituted or unsubstituted phenyl ring.
  • Ring system A may be substituted with one or more aryl group substituents as set forth in the definitions section herein.
  • the phenyl ring is substituted with a CN or methoxy moiety.
  • R 4 , R 4 ', R 5 , and R 3 ' links said drug to L 1 , if present, or to F, H, J, or X 2 , and R 3 is selected from SR 1 1 , NHR 1 1 and OR".
  • R 1 1 is selected from -SO(OH) 2 , -PO(OH) 2 , -AA n , -Si(CH 3 ) 2 C(CH 3 ) 3 , -C(O)OPhNH(AA) 1n ,
  • n is any integer in the range of 1 to 10
  • m is any integer in the range of 1 to 4
  • p is any integer in the range of 1 to 6
  • AA is any natural or non-natural amino acid.
  • AA n or AA 1n is selected from the same amino acid sequences described above for the peptide linkers (F) and optionally is the same as the amino acid sequence used in the linker portion of R 4 , R 4 ', R , or R '.
  • R" is cleavable in vivo to provide an active drug compound.
  • R 3 increases in vivo solublility of the compound.
  • the rate of decrease of the concentration of the active drug in the blood is substantially faster than the rate of cleavage of R ' to provide the active drug. This may be particularly useful where the toxicity of the active drag is substantially higher than that of the prodrug fo ⁇ n. In other embodiments, the rate of cleavage of R " to provide the active drug is faster than the rate of decrease of concentration of the active drug in the blood.
  • the invention provides a compound having a structure according to Formula (g):
  • the identities of the substituents R 3 , R 4 , R 4 ', R 5 , R 5 ⁇ R 6 , R 7 and X are substantially as described above for Formula (a), as well as preferences for particular embodiments.
  • the symbol Z is a member independently selected from O, S and NR" J .
  • the symbol R 23 represents a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl. Each R 23 is independently selected.
  • the symbol R 1 represents H, substituted or unsubstituted lower alkyl, or C(O)R 8 or CO 2 R 8 .
  • R 8 is a member selected from substituted alkyl, unsubstituted alkyl, NR 9 R 10 , NR 9 NHR 10 and OR 9 .
  • R 9 and R 10 are independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl.
  • R 2 is H, or substituted or unsubstituted lower alkyl. It is generally preferred that when R 2 is substituted alkyl, it is other than a perfluoroalkyl, e.g., CF 3 .
  • R 2 is a substituted alkyl wherein the substitution is not a halogen.
  • R 2 is an unsubstituted alkyl.
  • R 1 is an ester moiety, such as CO2CH3.
  • R 2 is a lower alkyl group, which may be substituted or unsubstituted. A presently preferred lower alkyl group is CH 3 .
  • R 1 is CO 2 CH 3 and R 2 is CH 3 .
  • R 4 , R 4 ', R 5 , and R 5 ' are members independently selected from H, halogen, NH 2 , OMe, O(CH 2 ) 2 N(R 29 ) 2 and NO 2 .
  • Each R 2q is independently H or lower alkyl (e.g., methyl).
  • the drug is selected such that the leaving group X 1 is a member selected from the group consisting of halogen, alkylsulfonyl, arylsulfonyl, and azide. In some embodiments, X 1 is F, Cl, or Br.
  • Z is O or NH.
  • X is O.
  • the invention provides compounds having a structure according to Formula (h) or (i):
  • duocarmycin analog of Formula (e) is a structure in which the ring system A is an unsubstituted or substituted phenyl ring.
  • the preferred substituents on the drug molecule described hereinabove for the structure of Formula 7 when the ring system A is a pyrrole are also preferred substituents when the ring system A is an unsubstituted or substituted phenyl ring.
  • the drug (D) comprises a structure (j):
  • R 3 , R 6 , R 7 , X are as described above for Formula (e).
  • Z is a member selected from O, S and NR 23 , wherein R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl;
  • R 1 is H, substituted or unsubstituted lower alkyl, C(O)R 8 , or CO 2 R 8 , wherein R 8 is a member selected from NR 9 R 10 and OR 9 , in which R 9 and R 10 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl;
  • R 1 is H, substituted or unsubstituted lower alkyl, or C(O)R 8 , wherein R 8 is a member selected from NR R 1 and OR , in which R y and R 10 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl;
  • R " is H, or substituted or unsubstituted lower alkyl or unsubstituted heteroalkyl or cyano or alkoxy; and R 2 is H, or substituted or unsubstituted lower alkyl or unsubstituted heteroalkyl.
  • At least one of R 4 , R »4- , R", R , R" , R . 1 1 2', R 1'3 J , R , 1"5 or R l b links the drug to L 1 , if present, or to F, H, J, or X 2 .
  • Another embodiment of the drug (D) comprises a structure (k) where R 4 and R 4 have been joined to from a heterocycloalkyl:
  • R 3 , R 5 , R 5 , R 6 , R 7 , X are as described above for Formula (e).
  • Z is a member selected from O, S and NR 23 , wherein R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl;
  • R 15 and R 16 independently represent H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl and substituted or unsubstituted peptidyl, where R 15 and R 16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms.
  • R 32 optionally contains one or more cleavable groups within its structure, such as a cleavable linker or cleavable substrate.
  • cleavable groups include, but are not limited to, peptides, amino acids, hydrazines, disulfides, and cephalosporin derivatives. Moreover, any selection of substituents described herein for R 4 , R 4 , R 5 , R 5 ', R' ⁇ and R 16 is also applicable to R ".
  • At least one of R 5 , R 5 ', R 1 ' 'R 12 , R 13 , R 15 , R 16 , or R 32 links the drug to L 1 , if present, or to F, H, J, or X 2 .
  • R 32 links the drug to L 1 , if present, or to F, H, J, or X 2 .
  • R is H, substituted or unsubstituted lower alkyl, C(O)R 8 , or CO 2 R , wherein R 8 is a member selected from NR 0 R 10 and OR 9 , in which R 9 and R 10 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl;
  • R 1 is H, substituted or unsubstituted lower alkyl, or C(O)R 8 , wherein R 8 is a member selected from NR 9 R 10 and OR 9 , in which R 9 and R 10 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl;
  • R" is H, or substituted or unsubstituted lower alkyl or unsubstituted heteroalkyl or cyano or alkoxy; and R ⁇ is H, or substituted or unsubstituted lower alkyl or unsubstituted heteroalkyl.
  • A, R 6 , R 7 , X, R 4 , R 4 , R 5 , and R 5 are as described above for Formula (e).
  • Z is a member selected from O, S and NR 23 , where R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl;
  • R is selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, halogen, NO 2 , NR 15 R 16 , NC(O)R 15 , OC(O)NR 15 R 16 , OC(O)OR 15 ,
  • R 13 and R 16 independently represent H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl and substituted or unsubstituted peptidyl, where R 15 and R 16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms.
  • R 33 links the drug to L 1 , if present, or to F, H, J, or X 2 .
  • A is substituted or unsubstituted phenyl or substituted or unsubstituted pyrrole.
  • any selection of substituents described herein for R is also applicable to R 33 .
  • X 4 represents a ligand selected from the group consisting of protected reactive functional groups, unprotected reactive functional groups, detectable labels, and targeting agents.
  • Preferred ligands are targeting agents, such as antibodies and fragments thereof.
  • the group X can be described as a member selected from R 29 , COOR 29 , C(O)NR 29 , and C(O)NNR 29 wherein R 29 is a member selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted heteroaryl.
  • R 29 is a thiol reactive member.
  • R 29 is a thiol reactive member selected from haloacetyl and alkyl halide derivatives, maleimides, aziridines, and acryloyl derivatives.
  • the above thiol reactive members can act as reactive protective groups that can be reacted with, for example, a side chain of an amino acid of a targeting agent, such as an antibody, to thereby link the targeting agent to the linker-drug moiety.
  • Detectable Labels The particular label or detectable group used in conjunction with the compounds and methods of the invention is generally not a critical aspect of the invention, as long as it does not significantly interfere with the activity or utility of the compound of the invention.
  • the detectable group can be any material having a detectable physical or chemical property.
  • a label is any composition detectable by spectroscopic, photochemical, biochemical, immunochemical, electrical, optical or chemical means.
  • Useful labels in the present invention include magnetic beads (e.g., DYNABEADS ⁇ M ), fluorescent dyes (e.g., fluorescein isothiocyanate, Texas red, rhodamine, and the like), radiolabels (e.g., 3 H, 125 1, 35 S, 14 C, or 32 P), enzymes (e.g., horse radish peroxidase, alkaline phosphatase and others commonly used in an ELISA), and colorimetric labels such as colloidal gold or colored glass or plastic beads (e.g., polystyrene, polypropylene, latex, etc.).
  • the label may be coupled directly or indirectly to a compound of the invention according to methods well known in the art.
  • the label is preferably a member selected from the group consisting of radioactive isotopes, fluorescent agents, fluorescent agent precursors, chromophores, enzymes and combinations thereof.
  • a detectable label that is frequently conjugated to an antibody is an enzyme, such as horseradish peroxidase, alkaline phosphatase, ⁇ - galactosidase, and glucose oxidase.
  • Non-radioactive labels are often attached by indirect means.
  • a ligand molecule e.g., biotin
  • the ligand then binds to another molecules (e.g., streptavidin) molecule, which is either inherently detectable or covalently bound to a signal system, such as a detectable enzyme, a fluorescent compound, or a chemiluminescent compound.
  • a signal system such as a detectable enzyme, a fluorescent compound, or a chemiluminescent compound.
  • Components of the conjugates of the invention can also be conjugated directly to signal generating compounds, e.g., by conjugation with an enzyme or fluorophore.
  • Enzymes of interest as labels will primarily be hydrolases, particularly phosphatases, esterases and glycosidases, or oxidotases, particularly peroxidases.
  • Fluorescent compounds include fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbel liferone, etc.
  • Chemiluminescent compounds include luciferin, and 2,3- dihydrophthalazinediones, e.g., luminol.
  • Means of detecting labels are well known to those of skill in the art.
  • means for detection include a scintillation counter or photographic film as in autoradiography.
  • the label is a fluorescent label, it may be detected by exciting the fluorochrome with the appropriate wavelength of light and detecting the resulting fluorescence. The fluorescence may be detected visually, by means of photographic film, by the use of electronic detectors such as charge coupled devices (CCDs) or photomultipliers and the like.
  • CCDs charge coupled devices
  • enzymatic labels may be detected by providing the appropriate substrates for the enzyme and detecting the resulting reaction product.
  • simple colorimetric labels may be detected simply by observing the color associated with the label. Thus, in various dipstick assays, conjugated gold often appears pink, while various conjugated beads appear the color of the bead.
  • Fluorescent labels are presently preferred as they have the advantage of requiring few precautions in handling, and being amenable to high-throughput visualization techniques (optical analysis including digitization of the image for analysis in an integrated system comprising a computer).
  • Preferred labels are typically characterized by one or more of the following: high sensitivity, high stability, low background, low environmental sensitivity and high specificity in labeling.
  • Many fluorescent labels are commercially available from the SIGMA chemical company (Saint Louis, MO), Molecular Probes (Eugene, OR), R&D systems (Minneapolis, MN), Pharmacia LKB Biotechnology (Piscataway, NJ), CLONTECH Laboratories, Inc.
  • fluorescent proteins include, for example, green fluorescent proteins of cnidarians (Ward et ai, Photochem. Photobiol. 35:803-808 (1982); Levine et al, Comp. Biochem. Physiol,
  • the chemical functionalities Prior to forming the linkage between the cytotoxin and the targeting (or other) agent, and optionally, the spacer group, at least one of the chemical functionalities will be activated.
  • the chemical functionalities including hydroxy, amino, and carboxy groups, can be activated using a variety of standard methods and conditions.
  • a hydroxyl group of the cytotoxin or targeting agent can be activated through treatment with phosgene to form the corresponding chloroformate, or p-nitrophenylchloroformate to form the corresponding carbonate.
  • the invention makes use of a targeting agent that includes a carboxyl functionality.
  • Carboxyl groups may be activated by, for example, conversion to the corresponding acyl halide or active ester. This reaction may be performed under a variety of conditions as illustrated in March, supra pp. 388-89.
  • the acyl halide is prepared through the reaction of the carboxyl- containing group with oxalyl chloride. The activated agent is reacted with a cytotoxin or cytotoxin-linker arm combination to form a conjugate of the invention.
  • carboxyl-containing targeting agents is merely illustrative, and that agents having many other functional groups can be conjugated to the linkers of the invention.
  • Exemplary compounds of the invention bear a reactive functional group, which is generally located on a substituted or unsubstituted alkyj.or heteroalkyl chain, allowing their facile attachment to another species.
  • a convenient location for the reactive group is the terminal position of the chain.
  • Reactive groups and classes of reactions useful in practicing the present invention are generally those that are well known in the art of bioconjugate chemistry.
  • the reactive functional group may be protected or unprotected, and the protected nature of the group may be changed by methods known in the art of organic synthesis.
  • Preferred classes of reactions available with reactive cytotoxin analogues are those which proceed under relatively mild conditions. These include, but are not limited to nucleophilic substitutions (e.g., reactions of amines and alcohols with acyl halides, active esters), electrophilic substitutions (e.g., enamine reactions) and additions to carbon-carbon and carbon- heteroatom multiple bonds (e.g., Michael reaction, Diels-Alder addition).
  • Exemplary reaction types include the reaction of carboxyl groups and various derivatives thereof including, but not limited to, N-hydroxysuccinimide esters, N- hydroxybenztriazole esters, acid halides, acyl imidazoles, thioesters, p-nitrophenyl esters, alkyl, alkenyl, alkynyl and aromatic esters.
  • Hydroxyl groups can be converted to esters, ethers, aldehydes, etc.
  • Haloalkyl groups are converted to new species by reaction with, for example, an amine, a carboxylate anion, thiol anion, carbanion, or an alkoxide ion.
  • Dienophile (e.g., maleimide) groups participate in Diels-Alder.
  • Aldehyde or ketone groups can be converted to imines, hydrazones, semicarbazones or oximes, or via such mechanisms as Grignard addition or alkyllithium addition.
  • Sulfonyl halides react readily with amines, for example, to form sulfonamides.
  • Amine or sulfhydryl groups are, for example, acylated, alkylated or oxidized.
  • Alkenes can be converted to an array of new species using cycloadditions, acylation, Michael addition, etc. Epoxides react readily with amines and hydroxyl compounds.
  • the reactive functional groups can be unprotected and chosen such that they do not participate in, or interfere with, the reactions. Alternatively, a reactive functional group can be protected from participating in the reaction by the presence of a protecting group. Those of skill in the art will understand how to protect a particular functional group from interfering with a chosen set of reaction conditions. For examples of useful protecting groups, See Greene et al., Protective Groups in Organic Synthesis, John Wiley & Sons, New York, 1991.
  • the targeting agent is linked covalently to a cytotoxin using standard chemical techniques through their respective chemical functionalities.
  • the linker or agent is coupled to the agent through one or more spacer groups.
  • the spacer groups can be equivalent or different when used in combination.
  • the invention comprises a carboxyl functionality as a reactive functional group.
  • Carboxyl groups may be activated as described hereinabove.
  • the cleavable substrates of the current invention are depicted as "X 2 ".
  • the cleavable substrate is a cleavable enzyme substrate that can be cleaved by an enzyme.
  • the enzyme is preferentially associated, directly or indirectly, with the tumor or other target cells to be treated.
  • the enzyme may be generated by the tumor or other target cells to be treated.
  • the cleavable substrate can be a peptide that is preferentially cleavable by an enzyme found around or in a tumor or other target cell.
  • the enzyme can be attached to a targeting agent that binds specifically to tumor cells, such as an antibody specific for a tumor antigen.
  • the peptide is cleavable by an enzyme, such as a trouase (such as thimet oligopeptidase), CDlO (neprilysin), a matrix metal lopro tease (such as MMP2 or MMP9), a type II transmembrane serine protease (such as Hepsin, testisin, TMPRSS4, or matriptase/MT- SPl), or a cathepsin, associated with a tumor.
  • a prodrug includes the drug as described above, a peptide, a stabilizing group, and optionally a linking group between the drug and the peptide.
  • the stabilizing group is attached to the end of the peptide to protect the prodrug from degradation before arriving at the tumor or other target cell.
  • suitable stabilizing groups include non-amino acids, such as succinic acid, diglycolic acid, maleic acid, polyethylene glycol, pyroglutamic acid, acetic acid, naphthylcarboxylic acid, terephthalic acid, and glutaric acid derivatives; as well as non-genetically-coded amino acids or aspartic acid or glutamic acid attached to the N- terminus of the peptide at the ⁇ -carboxy group of aspartic acid or the ⁇ -carboxyl group of glutamic acid.
  • the peptide typically includes 3-12 (or more) amino acids.
  • amino acids will depend, at least in part, on the enzyme to be used for cleaving the peptide, as well as, the stability of the peptide in vivo.
  • a suitable cleavable peptide is ⁇ -AlaLeuAlaLeu (SEQ ID NO: 102). This can be combined with a stabilizing group to form succinyl- ⁇ -AlaLeuAlaLeu (SEQ ID NO: 102).
  • SEQ ID NO: 102 ⁇ -AlaLeuAlaLeu
  • CDlO also known as neprilysin, neutral endopeptidase (NEP), and common acute lymphoblastic leukemia antigen (CALLA)
  • NEP neutral endopeptidase
  • CALLA common acute lymphoblastic leukemia antigen
  • Cleavable substrates suitable for use with CDlO include LeuAlaLeu and IleAlaLeu.
  • Other known substrates for CDlO include peptides of up to 50 amino acids in length, although catalytic efficiency often declines as the substrate gets larger.
  • MMP matrix metalloproteases
  • Suitable sequences for use with MMPs include, but are not limited to, ProValGlyLeuIleGly (SEQ ID NO: 95), GlyProLeuGlyVal (SEQ ID NO:96), GlyProLeuGlylleAlaGlyGln (SEQ ID NO: 97), ProLeuGlyLeu (SEQ ID NO: 98), GlyProLeuGlyMetLeuSerGln (SEQ ID NO: 99), and GlyProLeuGlyLeuTrpAlaGln (SEQ ID NO: 100).
  • ProValGlyLeuIleGly SEQ ID NO: 95
  • GlyProLeuGlyVal SEQ ID NO:96
  • GlyProLeuGlylleAlaGlyGln SEQ ID NO: 97
  • ProLeuGlyLeu SEQ ID NO: 98
  • GlyProLeuGlyMetLeuSerGln SEQ ID NO: 99
  • type II transmembrane serine proteases This group of enzymes includes, for example, hepsin, testisin, and TMPRSS4.
  • GlnAlaArg is one substrate sequence that is useful with matriptase/MT-SPl (which is over-expressed in breast and ovarian cancers) and LeuSerArg is useful with hepsin (over-expressed in prostate and some other tumor types).
  • Other cleavable substrates can also be used.
  • Another type of cleavable substrate arrangement includes preparing a separate enzyme capable of cleaving the cleavable substrate that becomes associated with the tumor or cells.
  • an enzyme can be coupled to a tumor-specific antibody (or other entity that is preferentially attracted to the tumor or other target cell such as a receptor ligand) and then the enzyme-antibody conjugate can be provided to the patient.
  • the enzyme-antibody conjugate is directed to, and binds to, antigen associated with the tumor.
  • the drug-cleavable substrate conjugate is provided to the patient as a prodrug.
  • the drug is only released in the vicinity of the tumor when the drug-cleavable substrate conjugate interacts with the enzyme that has become associated with the tumor so that the cleavable substrate is cleaved and the drug is freed.
  • suitable enzymes and substrates include, but are not limited to, ⁇ -lactamase and cephalosporin derivatives, carboxypeptidase G2 and glutamic and aspartic folate derivatives.
  • the enzyme-antibody conjugate includes an antibody, or antibody fragment, that is selected based on its specificity for an antigen expressed on a target cell, or at a target site, of interest.
  • an antibody or antibody fragment, that is selected based on its specificity for an antigen expressed on a target cell, or at a target site, of interest.
  • a discussion of antibodies is provided hereinabove.
  • One example of a suitable cephalosporin-cleavable substrate is
  • linkers and cleavable substrates of the invention can be used in conjugates containing a variety of partner molecules. Examples of conjugates of the invention are described in further detail below. Unless otherwise indicated, substituents are defined as set forth above in the sections regarding cytotoxins, linkers, and cleavable substrates.
  • Linker Conjugates One example of a suitable conjugate is a compound of the formula:
  • L 1 is a self-immolative linker
  • m is an integer 0, 1, 2, 3, 4, 5, or 6
  • F is a linker comprising the structure:
  • AA 1 is one or more members independently selected from the group consisting of natural amino acids and unnatural ⁇ - amino acids; c is an integer from 1 to 20; L 2 is a self-immolative linker and comprises
  • each R . 17 , R , 18 , and R , 19 i •s independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryi, and w is an integer from 0 to 4; o is 1; L 4 is a linker member; p is 0 or 1; X 4 is a member selected from the group consisting of protected reactive functional groups, unprotected reactive functional groups, detectable labels, and targeting agents; and D comprises a structure:
  • ring system A is a member selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups
  • E and G are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, a heteroatom, a single bond, or E and G are joined to form a ring system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl
  • X is a member selected from O, S and NR 23
  • R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl
  • R J is OR 1 ', wherein R 1 1 is a member selected from the group consisting of H, substituted alkyl, unsubsti
  • the drug has structure (c) or (f) above.
  • One specific example of a compound suitable for use as a conjugate is
  • L 1 is a self-immolative linker
  • m is an integer 0, 1, 2, 3, 4, 5, or 6
  • F is a linker comprising the structure: wherein AA is one or more members independently selected from the group consisting of natural amino acids and unnatural ⁇ -amino acids; c is an integer from 1 to 20; L 2 is a self-immolative linker; o is 0 or 1 ; L 4 is a linker member; p is 0 or 1 ; X 4 is a member selected from the group consisting of protected reactive functional groups, unprotected reactive functional groups, detectable labels, and targeting agents; and D comprises a structure:
  • ring system A is a member selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups
  • E and G are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, a heteroatom, a single bond, or E and G are joined to form a ring system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl
  • X is a member selected from O, S and NR 23
  • R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl
  • R 27 , R 27 , R 2S , and R 28 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl;
  • R 6 is a single bond which is either present or absent and when present R and R are joined to form a cyclopropyl ring; and R is CH 2 -X 1 or -CH 2 - joined in said cyclopropyl ring with R 6 , wherein X 1 is a leaving group.
  • the drug has structure (c) or (f) above.
  • r is an integer in the range from 0 to 24.
  • Another example of a suitable conjugate is a compound of the formula
  • L 4 is a linker member, wherein L 4 comprises
  • R >20 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl
  • each R 25 , R 25 , R 26 , and R 26 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl
  • s and t are independently integers from 1 to 6
  • p is 1
  • X 4 is a member selected from the group consisting of protected reactive functional groups, unprotected reactive functional groups, detectable labels, and targeting agents
  • D comprises a structure:
  • ring system A is a member selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups
  • E and G are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, a heteroatom, a single bond, or E and G are joined to form a ring system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl
  • X is a member selected from O, S and NR 23
  • R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl
  • the drug has structure (c) or (f) above.
  • One specific example of a compound suitable for use as conjugate is
  • r is an integer in the range from 0 to 24.
  • Suitable compounds for use as conjugates include:
  • R is oorr and r is an integer in the range from 0 to 24.
  • Conjugates can also be formed using the drugs having structure (g), such as the following compounds:
  • Conjugates can also be fo ⁇ ned using the drugs having the following structures:
  • the anti-CD 19 is conjugated to the linker and therapeutic agent of structure N 1 :
  • the anti-CD 19 is conjugated to the linker and therapeutic agent of structure N2: O
  • a suitable conjugate is a compound having the following structure: wherein L 1 is a self-immolative spacer; m is an integer of 0, 1, 2, 3, 4, 5, or 6; X 2 is a cleavable substrate; and D comprises a structure:
  • ring system A is a member selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups
  • E and G are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, a heteroatom, a single bond, or E and G are joined to form a ⁇ ng system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl
  • X is a member selected from O, S and NR 2 '
  • R 2 ' is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl
  • R 1 1 is a member selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, diphosphates, triphosphates, acyl, C(O)R 12 R 13 , C(O)OR 12 , C(O)NR 12 R 13 .
  • R 12 , R 13 , and R 14 are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryl, wherein R 12 and R 13 together with the nitrogen or carbon atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms; R 6 is a single bond which is either present or absent and when present R 6 and R 7 are joined to fo ⁇ n a cyclopropyl ring; and R 7 is CH 2 -X 1 or -CH 2 - joined in said cyclopropyl ring with R 6 , wherein X 1 is a leaving group, R 4 , R 4 ', R 5
  • R 15 and R 16 are independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, and substituted or unsubstituted peptidyl, wherein R 15 and R 16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or
  • cleavable linkers examples include ⁇ -AlaLeuAlaLeu (SEQ ID NO: 102) and
  • the present disclosure provides a composition, e.g., a pharmaceutical composition, containing one or a combination of monoclonal antibodies, or antigen-binding portion(s) thereof, of the present disclosure, formulated together with a pharmaceutically acceptable carrier.
  • Such compositions may include one or a combination of ⁇ e.g., two or more different) antibodies, or immunoconjugates or bispecific molecules of this disclosure.
  • a pharmaceutical composition of this disclosure can comprise a combination of antibodies (or immunoconjugates or bispecifics) that bind to different epitopes on the target antigen or that have complementary activities.
  • Pharmaceutical compositions of this disclosure also can be administered in combination therapy, i.e., combined with other agents.
  • the combination therapy can include an anti-CD 19 antibody of the present disclosure combined with at least one other anti-cancer agent. Examples of therapeutic agents that can be used in combination therapy are described in greater detail below in the section on uses of the antibodies of this disclosure.
  • pharmaceutically acceptable earner includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible.
  • the carrier is suitable for intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion).
  • the active compound i.e., antibody, immunoconjugate, or bispecific molecule
  • the pharmaceutical compounds of this disclosure may include one or more pharmaceutically acceptable salts.
  • a “pharmaceutically acceptable salt” refers to a salt that retains the desired biological activity of the parent compound and does not impart any undesired toxicological effects (see e.g.. Berge, S. M., et al. (1977) J. Pharm. Sci. 66: 1-19). Examples of such salts include acid addition salts and base addition salts.
  • Acid addition salts include those derived from nontoxic inorganic acids, such as hydrochloric, nitric, phosphoric, sulfuric, hydrobromic, hydroiodic, phosphorous and the like, as well as from nontoxic organic acids such as aliphatic mono- and dicarboxylic acids, phenyl- substituted alkanoic acids, hydroxy alkanoic acids, aromatic acids, aliphatic and aromatic sulfonic acids and the like.
  • nontoxic inorganic acids such as hydrochloric, nitric, phosphoric, sulfuric, hydrobromic, hydroiodic, phosphorous and the like
  • nontoxic organic acids such as aliphatic mono- and dicarboxylic acids, phenyl- substituted alkanoic acids, hydroxy alkanoic acids, aromatic acids, aliphatic and aromatic sulfonic acids and the like.
  • Base addition salts include those derived from alkaline earth metals, such as sodium, potassium, magnesium, calcium and the like, as well as from nontoxic organic amines, such as N.N'-dibenzylethylenediamine, N-methylglucamine, chloroprocaine, choline, diethanolamine, ethylenediamine, procaine and the like.
  • a pharmaceutical composition of this disclosure also may include a pharmaceutically acceptable anti-oxidant.
  • pharmaceutically acceptable antioxidants include: (1) water soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfite, sodium sulfite and the like; (2) oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA) 5 butylated hydroxytoluene (BHT), lecithin, propyl gallate, alpha-tocopherol, and the like; and (3) metal chelating agents, such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like.
  • water soluble antioxidants such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfite, sodium sulfite and the like
  • oil-soluble antioxidants such as ascorbyl palmitate, butylated
  • aqueous and nonaqueous carriers examples include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), and suitable mixtures thereof, vegetable oils, such as olive oil, and injectable organic esters, such as ethyl oleate.
  • polyols such as glycerol, propylene glycol, polyethylene glycol, and the like
  • vegetable oils such as olive oil
  • injectable organic esters such as ethyl oleate.
  • Proper fluidity can be maintained, for example, by the use of coating materials, such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.
  • compositions may also contain adjuvants such as preservatives, wetting agents, emulsifying agents and dispersing agents. Prevention of presence of microorganisms may be ensured both by sterilization procedures, supra, and by the inclusion of various antibacterial and antifungal agents, for example, paraben, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents which delay absorption such as aluminum monostearate and gelatin.
  • Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion.
  • sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion.
  • the use of such media and agents for pharmaceutically active substances is known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the pharmaceutical compositions of this disclosure is contemplated. Supplementary active compounds can also be incorporated into the compositions.
  • compositions typically must be sterile and stable under the conditions of manufacture and storage.
  • the composition can be formulated as a solution, microemulsion, liposome, or other ordered structure suitable to high drug concentration.
  • the carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof.
  • the proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.
  • isotonic agents for example, sugars, polyalcohols such as mannitol, sorbitol, or sodium chloride in the composition.
  • Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent that delays absorption, for example, monostearate salts and gelatin.
  • Sterile injectable solutions can be prepared by incoiporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by sterilization microfiltration.
  • dispersions are prepared by incorporating the active compound into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above.
  • the preferred methods of preparation are vacuum drying and freeze-drying (lyophilization) that yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
  • the amount of active ingredient which can be combined with a carrier material to produce a single dosage form will vary depending upon the subject being treated, and the particular mode of administration.
  • the amount of active ingredient which can be combined with a earner material to produce a single dosage form will generally be that amount of the composition which produces a therapeutic effect. Generally, out of one hundred per cent, this amount will range from about 0.01 per cent to about ninety-nine percent of active ingredient, preferably from about 0.1 per cent to about 70 per cent, most preferably from about 1 per cent to about 30 per cent of active ingredient in combination with a pharmaceutically acceptable carrier.
  • Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response). For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage.
  • Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
  • the dosage unit forms of this disclosure are dictated by and directly dependent on (a) the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and (b) the limitations inherent in the art of compounding such an active compound for the treatment of sensitivity in individuals.
  • the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to 5 mg/kg, of the host body weight.
  • dosages can be 0.3 mg/kg body weight, 1 mg/kg body weight, 3 mg/kg body weight, 5 mg/kg body weight or 10 mg/kg body weight or within the range of 1-10 mg/kg.
  • An exemplary treatment regime entails administration once per week, once every two weeks, once every three weeks, once every four weeks, once a month, once every 3 months or once every three to 6 months.
  • Preferred dosage regimens for an anti-CD 19 antibody of this disclosure include 1 mg/kg body weight or 3 mg/kg body weight via intravenous administration, with the antibody being given using one of the following dosing schedules: (i) every four weeks for six dosages, then every three months; (ii) every three weeks; (iii) 3 mg/kg body weight once followed by 1 mg/kg body weight every three weeks.
  • two or more monoclonal antibodies with different binding specificities are administered simultaneously, in which case the dosage of each antibody administered falls within the ranges indicated.
  • Antibody is usually administered on multiple occasions. Intervals between single dosages can be, for example, weekly, monthly, every three months or yearly. Intervals can also be irregular as indicated by measuring blood levels of antibody to the target antigen in the patient.
  • dosage is adjusted to achieve a plasma antibody concentration of about 1-1000 ⁇ g /ml and in some methods about 25-300 ⁇ g /ml.
  • antibody can be administered as a sustained release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the antibody in the patient. In general, human antibodies show the longest half life, followed by humanized antibodies, chimeric antibodies, and nonhuman antibodies. The dosage and frequency of administration can vary depending on whether the treatment is prophylactic or therapeutic. In prophylactic applications, a relatively low dosage is administered at relatively infrequent intervals over a long period of time. Some patients continue to receive treatment for the rest of their lives. In therapeutic applications, a relatively high dosage at relatively short intervals is sometimes required until progression of the disease is reduced or terminated, and preferably until the patient shows partial or complete amelioration of symptoms of disease. Thereafter, the patient can be administered a prophylactic regime. For use in the prophylaxis and/or treatment of diseases related to abnormal cellular proliferation, a circulating concentration of administered compound of about 0.001 ⁇ M to 20 ⁇ M is preferred, with about 0.01 ⁇ M to 5 ⁇ M being preferred.
  • Patient doses for oral administration of the compounds described herein typically range from about 1 mg/day to about 10,000 mg/day, more typically from about 10 mg/day to about 1,000 mg/day, and most typically from about 50 mg/day to about 500 mg/day. Stated in terms of patient body weight, typical dosages range from about 0.01 to about 150 mg/kg/day, more typically from about 0.1 to about 15 mg/kg/day, and most typically from about 1 to about 10 mg/kg/day, for example 5 mg/kg/day or 3 mg/kg/day. In at least some embodiments, patient doses that retard or inhibit tumor growth can be 1 ⁇ mol/kg/day or less.
  • the patient doses can be 0.9, 0.8, 0.7, 0.6, 0.5, 0.45, 0.3, 0.2, 0.15, 0.1, 0.09, 0.08, 0.07, 0.06, 0.05, 0.04, 0.03, 0.02, 0.01, or 0.005 ⁇ mol/kg or less (referring to moles of the drag).
  • the antibody-drug conjugate retards growth of the tumor when administered in the daily dosage amount over a period of at least five days.
  • the tumor is a human-type tumor in a SCID mouse.
  • the SCID mouse can be a CB 17.SCID mouse (available from Taconic, Ge ⁇ nantown, NY).
  • Actual dosage levels of the active ingredients in the pharmaceutical compositions of the present disclosure may be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient.
  • the selected dosage level will depend upon a variety of pharmacokinetic factors including the activity of the particular compositions of the present disclosure employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the medical arts.
  • a "therapeutically effective dosage" of an anti-CD 19 antibody of this disclosure preferably results in a decrease in severity of disease symptoms, an increase in frequency and duration of disease symptom-free periods, or a prevention of impairment or disability due to the disease affliction.
  • a "therapeutically effective dosage" of an anti-CD 19 antibody of this disclosure preferably results in a decrease in severity of disease symptoms, an increase in frequency and duration of disease symptom-free periods, or a prevention of impairment or disability due to the disease affliction.
  • a "therapeutically effective dosage" of an anti-CD 19 antibody of this disclosure preferably results in a decrease in severity of disease symptoms, an increase in frequency and duration of disease symptom-free periods, or a prevention of impairment or disability due to the disease affliction.
  • therapeutically effective dosage preferably inhibits cell growth or tumor growth by at least about 20%, more preferably by at least about 40%, even more preferably by at least about 60%, and still more preferably by at least about 80% relative to untreated subjects.
  • the ability of a compound to inhibit tumor growth can be evaluated in an animal model system predictive of efficacy in human tumors. Alternatively, this property of a composition can be evaluated by examining the ability of the compound to inhibit cell growth, such inhibition can be measured in vitro by assays known to the skilled practitioner.
  • a therapeutically effective amount of a therapeutic compound can decrease tumor size, or otherwise ameliorate symptoms in a subject.
  • One of ordinary skill in the art would be able to determine such amounts based on such factors as the subject's size, the severity of the subject's symptoms, and the particular composition or route of administration selected.
  • a composition of the present disclosure can be administered via one or more routes of administration using one or more of a variety of methods known in the art.
  • routes and/or mode of administration will vary depending upon the desired results.
  • Preferred routes of administration for antibodies of this disclosure include intravenous, intramuscular, intradermal, intraperitoneal, subcutaneous, spinal or other parenteral routes of administration, for example by injection or infusion.
  • parenteral administration means modes of administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, epidural and intrasternal injection and infusion.
  • an antibody of this disclosure can be administered via a non- parenteral route, such as a topical, epidermal or mucosal route of administration, for example, intranasally, orally, vaginally, rectally, sublingually or topically.
  • a non- parenteral route such as a topical, epidermal or mucosal route of administration, for example, intranasally, orally, vaginally, rectally, sublingually or topically.
  • the active compounds can be prepared with carriers that will protect the compound against rapid release, such as a controlled release formulation, including implants, transde ⁇ nal patches, and microencapsulated delivery systems.
  • a controlled release formulation including implants, transde ⁇ nal patches, and microencapsulated delivery systems.
  • Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods for the preparation of such formulations are patented or generally known to those skilled in the art. See, e.g., Sustained and Controlled Release Drug Delivery Systems, J. R. Robinson, edL, Marcel Dekker, Inc., New York, 1978.
  • Therapeutic compositions can be administered with medical devices known in the art.
  • a therapeutic composition of this disclosure can be administered with a needleless hypodermic injection device, such as the devices disclosed in U.S. Patent Nos. 5,399,163; 5,383,851; 5,312,335; 5,064,413; 4,941,880; 4,790,824; or 4,596,556.
  • a needleless hypodermic injection device such as the devices disclosed in U.S. Patent Nos. 5,399,163; 5,383,851; 5,312,335; 5,064,413; 4,941,880; 4,790,824; or 4,596,556.
  • Examples of well-known implants and modules useful in the present disclosure include: U.S. Patent No. 4,487,603, which discloses an implantable micro-infusion pump for dispensing medication at a controlled rate; U.S. Patent No. 4,486, 194, which discloses a therapeutic device for administering medicants through the skin; U.S. Patent No.
  • the blood-brain barrier excludes many highly hydrophilic compounds.
  • the therapeutic compounds of this disclosure cross the BBB (if desired)
  • they can be formulated, for example, in liposomes.
  • liposomes For methods of manufacturing liposomes, see, e.g., U.S. Patents 4,522,81 1 ; 5,374,548; and 5,399,331.
  • the liposomes may comprise one or more moieties which are selectively transported into specific cells or organs, thus enhance targeted drug delivery (see, e.g., V. V. Ranade (1989) J. Clin. Pharmacol. 29:685).
  • Exemplary targeting moieties include folate or biotin (see, e.g., U.S.
  • Patent 5,416,016 to Low et al. marmosides (Umezawa et ai, (1988) Biochem. Biophys. Res. Comrnun. 153:1038); antibodies (P.G. Bloeman et al. (1995) FEBS Lett. 357: 140; M. Owais et al (1995) Antimicrob. Agents Chemother. 39: 180); surfactant protein A receptor (Briscoe et al.
  • the antibodies particularly the human antibodies, antibody compositions antibody-partner molecule conjugate compositions and methods of the present disclosure have numerous in vitro and in vivo diagnostic and therapeutic utilities involving the diagnosis and treatment of CD 19 mediated disorders.
  • these molecules can be administered to cells in culture, in vitro or ex vivo, or to human subjects, e.g., in vivo, to treat, prevent and to diagnose a variety of disorders.
  • the term "subject" is intended to include human and non-human animals.
  • Non-human animals include all vertebrates, e.g., mammals and non-mammals, such as non-human primates, sheep, dogs, cats, cows, horses, chickens, amphibians, and reptiles.
  • Preferred subjects include human patients having disorders mediated by CD 19 activity.
  • the methods are particularly suitable for treating human patients having a disorder associated with aberrant CD 19 expression.
  • antibody-partner molecule conjugates to CD 19 are administered together with another agent, the two can be administered in either order or simultaneously.
  • the antibodies of this disclosure can be used to specifically detect CD 19 expression on the surface of cells and, moreover, can be used to purify CD 19 via immunoaff ⁇ nity purification.
  • the human antibody-partner molecule conjugate compositions and methods of the present disclosure can be used to treat a subject with a tumorigenic disorder, e.g., a disorder characterized by the presence of tumor cells expressing CD19 including, for example, non-Hodgkin's lymphoma (NHL), acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL), Burkitt's lymphoma, anaplastic large-cell lymphomas (ALCL), multiple myeloma, cutaneous T-cell lymphomas, nodular small cleaved-cell lymphomas, lymphocytic lymphomas, peripheral T-cell lymphomas, Lennert's lymphomas, immunoblastic lymphomas, T-cell leukemia/lymphomas (ATLL), adult T-cell leukemia (T-ALL), entroblastic/centrocytic (cb/cc) follicular lymphomas cancers
  • a tumorigenic disorder e.g
  • CD 19 may lead to loss of B-cell tolerance and generation of autoimmune disorders (Tedder et al. (2005) Curr Dir Autoimmun 8:55). This autoimmune effect has been seen by the accumulation of CD 19+ B-cells in the inflamed joints of rheumatoid arthritis patients (He et al. (2001 ) J Rheumatol 28:2168).
  • the human antibodies, antibody compositions and methods of the present disclosure can be used to treat a subject with an autoimmune disorder, e.g., a disorder characterized by the presence of B-cells expressing CD 19 including, for example, rheumatoid arthritis.
  • the antibodies e.g., human monoclonal antibodies, multispecific and bispecific molecules and compositions
  • the antibodies can be used to detect levels of CD 19, or levels of cells which contain CD 19 on their membrane surface, which levels can then be linked to certain disease symptoms.
  • the antibodies can be used to inhibit or block CD 19 function which, in turn, can be linked to the prevention or amelioration of certain disease symptoms, thereby implicating CD 19 as .a mediator of the disease. This can be achieved by contacting a sample and a control sample with the anti-CD 19 antibody under conditions that allow for the formation of a complex between the antibody and CD 19. Any complexes formed between the antibody and CD 19 are detected and compared in the sample and the control.
  • the antibodies (e.g., human antibodies, multispecif ⁇ c and bispecific molecules and compositions) of this disclosure can be initially tested for binding activity associated with therapeutic or diagnostic use in vitro.
  • compositions of this disclosure can be tested using the flow cytometric assays described in the Examples below.
  • the antibodies (e.g., human antibodies, multispecific and bispecific molecules, immunoconjugates and compositions) of this disclosure have additional utility in therapy and diagnosis of CD19-related diseases.
  • the human monoclonal antibodies, the multispecific or bispecific molecules and the immunoconjugates can be used to elicit in vivo or in vitro one or more of the following biological activities: to inhibit the growth of and/or kill a cell expressing CDl 9; to mediate phagocytosis or ADCC of a cell expressing CD 19 in the presence of human effector cells, or to block CD 19 ligand binding to CD 19.
  • the antibodies are used in vivo to treat, prevent or diagnose a variety of CD 19-related diseases.
  • CD 19-related diseases include, among others, autoimmune disorders, rheumatoid arthritis, cancer, non-Hodgkin's lymphoma, acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL), Burkitt's lymphoma, anaplastic large-cell lymphomas (ALCL), multiple myeloma, cutaneous T- cell lymphomas, nodular small cleaved-cell lymphomas, lymphocytic lymphomas, peripheral T-cell lymphomas, Lennert's lymphomas, immunoblastic lymphomas, T-cell leukemia/lymphomas (ATLL), adult T-cell leukemia (T-ALL), entroblastic/centrocytic (cb/cc) follicular lymphomas
  • ALL acute lymphocytic leukemia
  • CLL chronic lymphocytic leuk
  • Suitable routes of administering the antibody compositions of this disclosure e.g., human monoclonal antibodies, multispecific and bispecific molecules and immunoconjugates
  • the antibody compositions can be administered by injection (e.g., intravenous or subcutaneous).
  • Suitable dosages of the molecules used will depend on the age and weight of the subject and the concentration and/or formulation of the antibody composition.
  • human anti-CD 19 antibodies of this disclosure can be co-administered with one or other more therapeutic agents, e.g., a cytotoxic agent, a radiotoxic agent or an immunosuppressive agent.
  • the antibody can be linked to the agent (as an immunocomplex) or can be administered separate from the agent. In the latter case (separate administration), the antibody can be administered before, after or concurrently with the agent or can be co-administered with other known therapies, e.g., an anti-cancer therapy, e.g., radiation.
  • Such therapeutic agents include, among others, anti-neoplastic agents such as doxorubicin (adriamycin), cisplatin bleomycin sulfate, carmustine, chlorambucil, and cyclophosphamide hydroxyurea which, by themselves, are only effective at levels which are toxic or subtoxic to a patient.
  • anti-neoplastic agents such as doxorubicin (adriamycin), cisplatin bleomycin sulfate, carmustine, chlorambucil, and cyclophosphamide hydroxyurea which, by themselves, are only effective at levels which are toxic or subtoxic to a patient.
  • Cisplatin is intravenously administered as a 100 mg/ dose once every four weeks and adriamycin is intravenously administered as a 60-75 mg/ml dose once every 21 days.
  • Co-administration of the human anti-CD 19 antibodies, or antigen binding fragments thereof, of the present disclosure with chemotherapeutic agents provides two anti-cancer agents which operate via different mechanisms which yield a cytotoxic effect to human tumor cells.
  • Such co-administration can solve problems due to development of resistance to drugs or a change in the antigenicity of the tumor cells which would render them unreactive with the antibody.
  • Target-specific effector cells e.g., effector cells linked to compositions (e.g., human antibodies, multispecific and bispecific molecules) of this disclosure can also be used as therapeutic agents.
  • Effector cells for targeting can be human leukocytes such as macrophages, neutrophils or monocytes.
  • effector cells can be obtained from the subject to be treated.
  • the target-specific effector cells can be administered as a suspension of cells in a physiologically acceptable solution.
  • the number of cells administered can be in the order of 10 s - 10 9 but will vary depending on the therapeutic purpose. In general, the amount will be sufficient to obtain localization at the target cell, e.g., a tumor cell expressing CD 19, and to effect cell killing by, e.g., phagocytosis. Routes of administration can also vary. Therapy with target-specific effector cells can be performed in conjunction with other techniques for removal of targeted cells.
  • anti-tumor therapy using the compositions (e.g., human antibodies, multispecif ⁇ c and bispecific molecules) of this disclosure and/or effector cells armed with these compositions can be used in conjunction with chemotherapy.
  • combination immunotherapy may be used to direct two distinct cytotoxic effector populations toward tumor cell rejection.
  • anti-CD 19 antibodies linked to anti-Fc-gamma RI or anti-CD3 may be used in conjunction with IgG- or IgA-receptor specific binding agents.
  • Bispecific and multispecific molecules of this disclosure can also be used to modulate Fc ⁇ R or Fc ⁇ R levels on effector cells, such as by capping and elimination of receptors on the cell surface. Mixtures of anti-Fc receptors can also be used for this purpose.
  • compositions ⁇ e.g., human antibodies, multispecific and bispecific molecules and immunoconjugates) of this disclosure which have complement binding sites, such as portions from IgGl, -2, or -3 or IgM which bind complement, can also be used in the presence of complement.
  • ex vivo treatment of a population of cells comprising target cells with a binding agent of this disclosure and appropriate effector cells can be supplemented by the addition of complement or serum containing complement.
  • Phagocytosis of target cells coated with a binding agent of this disclosure can be improved by binding of complement proteins.
  • target cells coated with the compositions (e.g., human antibodies, multispecific and bispecific molecules) of this disclosure can also be lysed by complement.
  • the compositions of this disclosure do not activate complement.
  • compositions of this disclosure can also be administered together with complement.
  • the instant disclosure provides compositions comprising human antibodies, multispecific or bispecific molecules and serum or complement. These compositions can be advantageous when the complement is located in close proximity to the human antibodies, multispecific or bispecific molecules.
  • the human antibodies, multispecific or bispecific molecules of this disclosure and the complement or serum can be administered separately.
  • kits which comprise the antibody compositions of this disclosure (e.g., human antibodies, bispecific or multispecific molecules, or immunoconjugates), and instructions for its use.
  • the kit can further contain one or more additional reagents, such as an immunosuppressive reagent, a cytotoxic agent or a radiotoxic agent, or one or more additional human antibodies of this disclosure (e.g., a human antibody having a complementary activity which binds to an epitope in the CD 19 antigen distinct from the first human antibody).
  • additional reagents such as an immunosuppressive reagent, a cytotoxic agent or a radiotoxic agent, or one or more additional human antibodies of this disclosure (e.g., a human antibody having a complementary activity which binds to an epitope in the CD 19 antigen distinct from the first human antibody).
  • patients treated with antibody compositions of this disclosure can be additionally administered (prior to, simultaneously with, or following administration of a human antibody of this disclosure) with another therapeutic agent, such as a cytotoxic or radiotoxic agent, which enhances or augments the therapeutic effect of the human antibodies.
  • another therapeutic agent such as a cytotoxic or radiotoxic agent, which enhances or augments the therapeutic effect of the human antibodies.
  • the subject can be additionally treated with an agent that modulates, e.g., enhances or inhibits, the expression or activity of Fc ⁇ or Fc ⁇ receptors by, for example, treating the subject with a cytokine.
  • cytokines for administration during treatment with the multispecific molecule include of granulocyte colony-stimulating factor (G-CSF), granulocyte- macrophage colony-stimulating factor (GM-CSF), interferon- ⁇ (IFN- ⁇ ), and tumor necrosis factor (TNF).
  • G-CSF granulocyte colony-stimulating factor
  • GM-CSF granulocyte- macrophage colony-stimulating factor
  • IFN- ⁇ interferon- ⁇
  • TNF tumor necrosis factor
  • compositions e.g., human antibodies, multispecific and bispecific molecules
  • the compositions can also be used to target cells expressing Fc ⁇ R or CD 19, for example for labeling such cells.
  • the binding agent can be linked to a molecule that can be detected.
  • this disclosure provides methods for localizing ex vivo or in vitro cells expressing Fc receptors, such as Fc ⁇ R, or CD 19.
  • the detectable label can be, e.g., a radioisotope, a fluorescent compound, an enzyme, or an enzyme co-factor.
  • this disclosure provides methods for detecting the presence of CD 19 antigen in a sample, or measuring the amount of CD 19 antigen, comprising contacting the sample, and a control sample, with a human monoclonal antibody, or an antigen binding portion thereof, which specifically binds to CD 19, under conditions that allow for formation of a complex between the antibody or portion thereof and CD 19. The formation of a complex is then detected, wherein a difference complex formation between the sample compared to the control sample is indicative the presence of CD 19 antigen in the sample.
  • this disclosure provides methods for treating an CD 19 mediated disorder in a subject, e.g., autoimmune disorder, rheumatoid arthritis, cancer, non-Hodgkin's lymphoma, acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL), Burkitt's lymphoma, anaplastic large-cell lymphomas (ALCL), cutaneous T-cell lymphomas, nodular small cleaved-cell lymphomas, lymphocytic lymphomas, peripheral T-cell lymphomas, Lennert's lymphomas, immunoblastic lymphomas, T-cell leukemia/lymphomas (ATLL), adult T-cell leukemia (T-ALL), entroblastic/centrocytic (cb/cc) follicular lymphomas cancers, diffuse large cell lymphomas of B lineage, angioimmunoblastic lymphadenopathy (AILD)-like T cell lymphoma, HIV associated
  • ALL acute
  • Such antibodies and derivatives thereof are used to inhibit CD 19 induced activities associated with certain disorders, e.g., proliferation and differentiation.
  • CD 19 By contacting the antibody with CD 19 (e.g., by administering the antibody to a subject), the ability of CD 19 to induce such activities is inhibited and, thus, the associated disorder is treated.
  • the antibody composition can be administered alone or along with another therapeutic agent, such as a cytotoxic or a radiotoxic agent which acts in conjunction with or synergistically with the antibody composition to treat or prevent the CD 19 mediated disease.
  • immunoconjugates of this disclosure can be used to target compounds (e.g., therapeutic agents, labels, cytotoxins, radiotoxins immunosuppressants, etc.) to cells which have CD 19 cell surface receptors by linking such compounds to the antibody.
  • compounds e.g., therapeutic agents, labels, cytotoxins, radiotoxins immunosuppressants, etc.
  • an anti-CD 19 antibody can be conjugated to any of the toxin compounds described in US Patent Nos. 6, 281, 354 and 6,548,530, US patent publication Nos. 20030050331, 20030064984, 20030073852, and 20040087497, or published in WO 03/022806.
  • this disclosure also provides methods for localizing ex vivo or in vivo cells expressing CD 19 (e.g., with a detectable label, such as a radioisotope, a fluorescent compound, an enzyme, or an enzyme co- factor).
  • a detectable label such as a radioisotope, a fluorescent compound, an enzyme, or an enzyme co- factor.
  • the immunoconjugates can be used to kill cells which have CD 19 cell surface receptors by targeting cytotoxins or radiotoxins to CD 19.
  • the B cell tumor cell lines Raji (ATCC Accession #CCL-86) and Daudi (ATCC Accession #CCL-213) were used as antigen for immunization.
  • Fully human monoclonal antibodies to CD 19 were prepared using the KM strain of transgenic transchromosomic mice, which expresses human antibody genes.
  • the endogenous mouse kappa light chain gene has been homozygously disrupted as described in Chen et al. ( 1993) EMBO J. 12:81 1 -820 and the endogenous mouse heavy chain gene has been homozygously disrupted as described in Example 1 of PCT Publication WO 01/09187 for HuMab mice.
  • the mouse carries a human kappa light chain transgene, KCo5, as described in Fishwild et al. (1996) Nature Biotechnology 14:845-851.
  • the mouse also carries a human heavy chain transchromosome, SC20, as described in PCT Publication WO 02/43478.
  • mice were 6-16 weeks of age upon the first infusion of antigen.
  • a cell preparation was used to immunize the mice (KM-MOUSE ® ) intraperitonealy (IP).
  • mice were immunized twice with antigen in complete Freund's adjuvant or Ribi adjuvant IP, followed by 3-21 days IP (up to a total of 1 1 immunizations) with the antigen in incomplete Freund's or Ribi adjuvant.
  • the immune response was monitored by retroorbital bleeds.
  • the plasma was screened by ELISA (as described below), and mice with sufficient titers of anti-CD 19 human immunogolobulin were used for fusions. Mice were boosted intravenously with antigen 3 days before sacrifice and removal of the spleen. Selection of a KM-MOUSE ® Producing Anti-CD 19 Antibodies:
  • the plates were washed with PBS/Tween and then incubated with a goat- anti-human kappa light chain polyclonal antibody conjugated with alkaline phophatase for 1 hour at room temperature. After washing, the plates were developed with pNPP substrate and analyzed by spectrophotometer at OD 415-650. Mice that developed the highest titers of anti-CD 19 antibodies were used for fusions. Fusions were performed as described below and hybridoma supernatants were tested for anti-CD 19 activity by ELISA. Generation of Hybridomas Producing Human Monoclonal Antibodies to CD 19:
  • mice splenocytes isolated from a KM-MOUSE* " , were fused with PEG to a mouse myeloma cell line either using PEG based upon standard protocols or electric field based electrofusion using a Cyto Pulse large chamber cull fusion electroporator (Cyto Pulse Sciences, Inc., Glen Burnie, MD). The resulting hybridomas were then screened for the production of antigen-specific antibodies. Single cell suspensions of splenic lymphocytes from immunized mice were fused to one-fourth the number of SP2/0 nonsecreting mouse myeloma cells (ATCC, CRL 1581) with 50% PEG (Sigma).
  • Cells were plated at approximately lxlO 5 /well in flat bottom microtiter plate, followed by about two week incubation in selective medium containing 10% fetal bovine serum, 10% P388D1 (ATCC, CRL TIB-63) conditioned medium, 3-5% origen (IGEN) in DMEM (Mediatech, CRL 10013, with high glucose, L-glutamine and sodium pyruvate) plus 5 mM HEPES, 0.055 mM 2-mercaptoethanol, 50 mg/ml gentamycin and Ix HAT (Sigma, CRL P-7185). After 1-2 weeks, cells were cultured in medium in which the HAT was replaced with HT.
  • selective medium containing 10% fetal bovine serum, 10% P388D1 (ATCC, CRL TIB-63) conditioned medium, 3-5% origen (IGEN) in DMEM (Mediatech, CRL 10013, with high glucose, L-glutamine and sodium pyruvate) plus 5 mM HEPES,
  • Example 2 Structural Characterization of Human Monoclonal Antibodies 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8
  • the cDNA sequences encoding the heavy and light chain variable regions of the 21D4 and 21D4a monoclonal antibodies were obtained from the 21D4 hybridoma using standard PCR techniques and were sequenced using standard DNA sequencing techniques. It is noted that the 21D4 hybridoma produces antibodies having a heavy chain that pairs with one of two light chains (SEQ ID NOs: 8 and 9). Both antibodies (i.e., 21D4 with V H and V L sequences of SEQ ID NOs: 1 and 8, respectively, and 21D4a with V H and V 1 sequences of SEQ ID NOs: 1 and 9, respectively) bind to CD 19.
  • the cDNA sequences encoding the heavy and light chain variable regions of the 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 monoclonal antibodies were obtained from the 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 hybridomas, respectively, using standard PCR techniques and were sequenced using standard DNA sequencing techniques.
  • 21D4 are shown in Figure IA and in SEQ ID NO: 59 and 1, respectively.
  • nucleotide and amino acid sequences of the light chain variable region of 21D4 are shown in Figure IB and in SEQ ID NO: 66 and 8, respectively.
  • 21D4 heavy chain immunoglobulin sequence Comparison of the 21D4 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 21 D4 heavy chain utilizes a V H segment from human germline V H 5-51, a D segment from the human germline 3-10, and a J H segment from human germline JH 4b.
  • the alignment of the 21D4 V H sequence to the germline V H 5-51 sequence is shown in Figure 8.
  • Further analysis of the 21D4 Vn sequence using the Kabat system of CDR region determination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures IA and 8, and in SEQ ID NOs: 16, 23 and 30, respectively.
  • nucleotide and amino acid sequences of the heavy chain variable region of 21D4a are shown in Figure IA and in SEQ ID NO: 59 and 1, respectively.
  • the nucleotide and amino acid sequences of the light chain variable region of 21D4a are shown in Figure 1C and in SEQ ID NO: 67 and 9, respectively.
  • Comparison of the 21D4a heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 21D4a heavy chain utilizes a Vn segment from human germline V H 5-51, a D segment from the human germline 3-10, and a J H segment from human germline JH 4b.
  • the alignment of the 21D4a V H sequence to the germline V ( 1 5-51 sequence is shown in Figure 8.
  • the nucleotide and amino acid sequences of the light chain variable region of 47G4 are shown in Figure 2B and in SEQ ID NO: 68 and 10, respectively. Comparison of the 47G4 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 47G4 heavy chain utilizes a V H segment from human germline V H 1-69, a D segment from the human germline 6-19, and a J H segment from human germline JH 5b. The alignment of the 47G4 V H sequence to the germline V H 1 -69 sequence is shown in Figure 9.
  • nucleotide and amino acid sequences of the heavy chain variable region of 27F3 are shown in Figure 3A and in SEQ ID NO: 61 and 3, respectively.
  • the nucleotide and amino acid sequences of the light chain variable region of 27F3 are shown in Figure 3B and in SEQ ID NO: 69 and 1 1, respectively.
  • Comparison of the 27F3 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 27F3 heavy chain utilizes a V H segment from human germline V H 5-51, a D segment from the human germline 6-19, and a J H segment from human germline JH 6b.
  • the alignment of the 27F3 V H sequence to the germline V H 5-51 sequence is shown in Figure 10.
  • nucleotide and amino acid sequences of the heavy chain variable region of 3C10 are shown in Figure 4A and in SEQ ID NO: 62 and 4, respectively.
  • the nucleotide and amino acid sequences of the light chain variable region of 3ClO are shown in Figure 4B and in SEQ ID NO: 70 and 12, respectively.
  • Comparison of the 3C10 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 3C10 heavy chain utilizes a V H segment from human germline VH 1-69, a D segment from the human germline 1-26, and a J H segment from human germline JH 6b.
  • the alignment of the 3C10 V H sequence to the ge ⁇ nline V H 1-69 sequence is shown in Figure 1 1.
  • the nucleotide and amino acid sequences of the light chain variable region of 5G7 are shown in Figure 5B and in SEQ ID NO: 71 and 13, respectively. Comparison of the 5G7 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 5G7 heavy chain utilizes a V H segment from human germline V H 5-51, a D segment from the human germline 3-10, and a J H segment from human germline JH 6b. The alignment of the 5G7 V H sequence to the germline V H 5-51 sequence is shown in Figure 12.
  • nucleotide and amino acid sequences of the heavy chain variable region of 13Fl are shown in Figure 6A and in SEQ ID NO: 64 and 6, respectively.
  • the nucleotide and amino acid sequences of the light chain variable region of 13Fl are shown in Figure 6B and in SEQ ID NO: 72 and 14, respectively. Comparison of the 13Fl heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 13Fl heavy chain utilizes a V H segment from human germline V H 5-51, a D segment from the human germline 6-19, and a Jn segment from human germline JH 6b. The alignment of the 13Fl V H sequence to the germline Vn 5-51 sequence is shown in Figure 13.
  • nucleotide and amino acid sequences of the heavy chain variable region of 46E8 are shown in Figure 7A and in SEQ ID NO: 65 and 7, respectively.
  • the nucleotide and amino acid sequences of the light chain variable region of 46E8 are shown in Figure 7B and in SEQ ID NO: 73 and 15, respectively. Comparison of the 46E8 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 46E8 heavy chain utilizes a V H segment from human germline V H 5-51 , a D segment from the human germline 6-19, and a J H segment from human germline JH 6b. The alignment of the 46E8 Vn sequence to the germline V H 5-51 sequence is shown in Figure 14.
  • binding affinity of the anti-CD 19 antibodies 21D4 and 47G4 were examined by ELISA analysis. Binding specificity by ELISA
  • Microtiter plates were coated with 50 ⁇ l purified full-length CD 19-Fc fusion protein at 1.0 ⁇ g/ml in PBS, and then blocked with 150 ⁇ l of 1% bovine serum albumin in PBS. The plates were allowed to incubate for 30 minutes to 1 hour and washed three times. Dilutions of the HuMAb anti-CD 19 antibody 47G4 was added to each well and incubated for 1 hour at 37 0 C. A known murine anti-CD 19 antibody was used as a positive control. The plates were washed with PBS/Tween and then incubated with a goat anti-human IgG Kappa-specific secondary reagent conjugated to horseradish peroxidase for 1 hour at 37 0 C.
  • Flow cytometry was used to determine epitope grouping of anti-CD 19 HuMAbs.
  • Epitope binding of the anti-CD 19 human monoclonal antibodies 21D4, 21D4a, 3C10, 5G7, 5G7-N19K, 5G7-N19Q and 13Fl was assessed by incubating Raji B tumor cells with 0.3 ⁇ g/ml of either biotinylated 21 D4 or 21 D4a anti-CD 19 human monoclonal antibody, washed, and followed by the addition of a cold anti-CD 19 human monoclonal antibody.
  • An isotype control antibody was used as a negative control. Binding was detected with a FITC-labeled anti-human IgG Ab.
  • CD 19 HuMAbs Binding of the CD 19 HuMAbs by flow cytometry to the B cell tumor lines Raji and Daudi, or to a CHO-CD 19 transfected cell line was assessed.
  • CHO cells were transfected with an expression plasmid containing the full length cDNA encoding the transmembrane form of CD 19.
  • the Raji, Daudi, and CD19-CHO cell lines were incubated with one of the following CD 19 HuMAbs: 21D4, 21D4a, 47G4, 5G7, 5G7- N 19K, 5G7-N19Q, 3C10 or 13Fl .
  • a known murine anti-CD 19 antibody was used as a positive control.
  • the cells were washed and detected by either a phycoerythrin-labeled anti-human or anti-mouse secondary antibody and analyzed by flow cytometry.
  • the results for binding to the CHO-CD 19 cell line, Daudi B cell line, Raji B cell line and an expanded binding set against the Raji B cell line are shown in Figures 25 A, 25B, 25C and 25D, respectively.
  • the human anti-CD 19 monoclonal antibodies, 21D4 and 47G4 bound to the CHO-CD 19 cell line.
  • the anti-CD19 HuMAb antibodies 21 D4, 21D4a, 3C10, 5G7, 5G7-N19K, 5G7- N19Q, and 13Fl had calculated EC 50 values of 0.1413, 0.1293, 0.2399, 0.1878. 0.2240, 0.2167 and 0.2659, respectively. 47G4 was also shown to bind the Daudi B tumor cell line. AU results are shown as measured by the geometric mean fluorescent intensity (GMFI) of staining.
  • GMFI geometric mean fluorescent intensity
  • CD 19 protein is expressed on the surface of tumor cell lines of B cell origin and that the anti-CD 19 HuMAb antibodies 21D4, 21D4a, 47G4, 5G7, 5G7-N19K, 5G7-N19Q, 3C10 and 13Fl bind to CD19 expressed on the cell surface.
  • Raji cells were obtained from ATCC (Accession #CCL-86) and grown in RPMI containing 10% fetal bovine serum (FBS). The cells were washed twice with RPMI containing 10% FBS at 4 0 C and the cells were adjusted to 4x10 7 cells/ml in RPMI media containing 10% fetal bovine serum (binding buffer containing 24mM Tris pH 7.2, 137mM NaCl, 2.7mN KCl, 2mM glucose, ImM CaCl 2 , ImM MgCl 2 , 0.1% BSA). Millipore plates (MAFB NOB) were coated with 1% nonfat dry milk in water and stored a 4 0 C overnight.
  • FBS fetal bovine serum
  • the plates were washed with binding buffer and 25 ⁇ l of unlabeled antibody (1000-fold excess) in binding buffer was added to control wells in a Millipore 96 well glass fiber filter plate (non-specific binding NSB). Twenty-five microliters of buffer alone was added to the maximum binding control well (total binding). Twenty- five microliters of varying concentrations of l2:> I-anti-CD19 antibody 21D4 or 47G4 and 25 ⁇ l of Raji cells (4 X 10 7 cells/ml) in binding buffer were added. The plates were incubated for 2 hours at 200 RPM on a shaker at 4 0 C.
  • the Millipore plates were washed three times with 0.2 ml of cold wash buffer (24mM Tris pH 7.2, 50OmM NaCl, 2.7mN KCl, 2mM glucose, ImM CaCl 2 , ImM MgCl 2 , 0.1% BSA). The filters were removed and counted in a gamma counter. Evaluation of equilibrium binding was performed using single site binding parameters with the Prism software (San Diego, CA). Using the above scatchard binding assay, the K D of the antibody for Raji cells was approximately 2.14 nM for 21D4 and 12.02 nM for 47G4.
  • Anti-CD 19 HuMAbs were tested for the ability to internalize into CD 19- expressing Raji B tumor cells or human CHO cells transfected with CD 19 using a Hum- Zap internalization assay.
  • Hum-Zap tests for internalization of a primary human antibody through binding of a secondary antibody with affinity for human IgG conjugated to the toxin saporin.
  • the CHO-CD 19 or Raji B tumor cell line was seeded at 1.0x10 4 cells/well in 100 ⁇ l wells either overnight or the following day for a two hour period. Either the anti- CD 19 antibody 21 D4 or 47G4 were added to the wells at a starting concentration of 30 nM and titrated down at 1:3 serial dilutions. A human isotype control antibody that is non-specific for CD 19 was used as a negative control.
  • the Hum-Zap Advanced Targeting Systems, IT-22-25
  • anti-CD 19 monoclonal antibodies conjugated to a cytotoxin were tested for the ability to kill CD 19+ cell lines in a thymidine incorporation assay. Cytotoxin N 1 was used in this experiment. An anti-CD 19 monoclonal antibody was conjugated to a cytotoxin via a linker, such as a peptidyl, hydrazone or disulfide linker. The CD 19+ expressing Raji cell line was seeded at 2.5x10 4 cells/wells for 3 hours. An anti-CD 19 antibody-cytotoxin conjugate was added to the wells at a starting concentration of 30 nM and titrated down at 1 :3 serial dilutions.
  • An isotype control antibody that is non-specific for CDl 9 was used as a negative control. Ten-fold excess cold antibody, either 21 D4a or an isotype control antibody is used to compete binding. Plates were allowed to incubate for 69 hours. The plates were then pulsed with 1.0 ⁇ Ci of 3 H-thymidine for 24 hours, harvested and read in a Top Count Scintillation Counter (Packard Instruments, Meriden, CT). The results are shown in Figure 27A and B along with the EC50 values. This data demonstrates that the anti-CD 19 antibody 21 D4 kills Raji B-cell tumor cells.
  • Example 8 Treatment of in vivo B cell Tumors Using Anti-CD19 Antibodies
  • SCID mice implanted with cancerous B cell tumors were treated in vivo with either naked anti-CD19 21D4 antibodies or cytotox in-conjugated anti-CD19 antibody 21D4 to examine the in vivo effect of the antibodies on tumor growth.
  • Cytotoxin N 1 was used in this experiment.
  • Cytotoxin-conjugated anti-CD 19 antibodies were prepared as described above. Severe combined immune deficient (SCID) mice, which lack functional B and T lymphocytes were used to study B-cell malignancies. Cells from the Ramos B tumor cell line were injected intravenously. The mice were treated either with 19.6 mg/kg of cytotox in-conjugate anti-CD 19 antibody or 30 mg/kg naked anti-CD 19 antibody. An isotype control antibody or formulation buffer was used as a negative control. The isotype control was conjugated to the free toxin released by cleavage of the linker in Nl. The animals were dosed by intraperitoneal injection with approximately 200 ⁇ l of PBS containing antibody or vehicle.
  • SCID Severe combined immune deficient mice, which lack functional B and T lymphocytes were used to study B-cell malignancies. Cells from the Ramos B tumor cell line were injected intravenously. The mice were treated either with 19.6 mg/kg of cytotox in-conjugate anti
  • the antibody-cytotoxin conjugate was injected as a single dose on day 7, while the naked antibody was either injected as a single dose prophylactic model on day 1 or as a treatment model on days 7, 14 and 21.
  • the mice were monitored daily for hind leg paralysis for approximately 6 weeks. Using an electronic caliper, the tumors were measured three dimensionally (height x width x length) and tumor volume was calculated. Mice were euthanized when there was hindleg paralysis.
  • the change in body weight was also measured and calculated as percent change in weight.
  • the data is shown in Figures 29A and B. Over a 30 day period, there was a net increase change in body weight with one cytotoxin-conjugate antibody and a net decrease change in body weight with antibody and cytotoxin (not conjugate). There was a net increase change in body weight with either the prophylactic naked anti-CD 19 antibody or the anti-CD 19 antibody treatment regimen.
  • mice implanted with a lymphoma tumor were treated in vivo with naked anti- CD ⁇ antibodies to examine the in vivo effect of the antibodies on tumor growth.
  • ARH-77 human B lymphoblast leukemia; ATCC Accession No. CRL- 1621
  • Raji human B lymphocyte Burkitt's lymphoma; ATCC Accession No. CCL-86 cells were expanded in vitro using standard laboratory procedures.
  • SCID mice Teconic, Hudson, NY
  • Mice were weighed and measured for tumors three dimensionally using an electronic caliper twice weekly after implantation. Tumor volumes were calculated as height x width x length/2.
  • mice with ARH-77 tumors averaging 80 mm 3 or Raji tumors averaging 170 mm 3 were randomized into treatment groups.
  • the mice were dosed intraperitoneal Iy with PBS vehicle, isotype control antibody or naked anti-CD 19 HuMAb 2H5 on Day 0. Mice were euthanized when the tumors reached tumor end point (2000 mm 3 ).
  • the results are shown in Figure 30A (ARH-77 tumors) and 30B (Raji tumors).
  • the naked anti-CD 19 antibody 21 D4 extended the mean time to reaching the tumor end point volume (2000 mm 3 ) and slowed tumor growth progression.
  • treatment with an anti-CD 19 antibody alone has a direct in vivo inhibitory effect on tumor growth.
  • Antibodies with reduced amounts of fucosyl residues have been demonstrated to increase the ADCC ability of the antibody.
  • the anti-CD 19 HuMAb 21D4 has been produced that is lacking in fucosyl residues.
  • the CHO cell line Ms704-PF which lacks the fucosyltransferase gene, FUT 8 (Biowa, Inc., Princeton, NJ) was electroporated with a vector which expresses the heavy and light chains of antibody 21 D4. Drug-resistant clones were selected by growth in Ex- Cell 325-PF CHO media (JRH Biosciences, Lenexa, KS) with 6 mM L-glutamine and
  • the N-linked glycans from the Fc portion of HuMAb 21 D4 expressed in CHO fucosylating and non-fucosylating cells were released.
  • the supernatant containing the glycans was dried by vacuum centrifugation and resuspended in 19mM 8-aminopyrene- 1,3,6- trisulfonate (APTS) (Beckman) under mild reductive amination conditions in which desialylation and loss of fucose residues was minimized (15% acetic acid and 1 M sodium cyanoborohydride in THF (Sigma)).
  • APTS 19mM 8-aminopyrene- 1,3,6- trisulfonate
  • the glycan labeling reaction was allowed to continue overnight at 40°C followed by 25-fold dilution of sample in water.
  • APTS- labeled glycans were applied to capillary electrophoresis with laser induced fluorescence on a P/ACE MDQ CE system (Beckman) with reverse polarity, using a 50 ⁇ m internal diameter N-CHO coated capillary (Beckman) with 50 cm effective length. Samples were pressure (8 sec.) injected and separation was carried out at 20°C using Carbohydrate Separation Gel Buffer (Beckman) at 25 kV for 20 min.
  • IgG samples (200 ⁇ g) were subjected to acid hydrolysis using either 2 N TFA (for estimating neutral sugars) or 6 N HCl (for estimating amino sugars) at 100°C for 4 h. Samples were dried by vacuum centrifugation at ambient temperature and were reconstituted in 200 ⁇ l water prior to analysis by HPAE-PAD (Dionex). Monosaccharides were separated using a CarboPac PAlO 4 x 250 mm column with pre- column Amino Trap and Borate Trap (Dionex). Procedures were followed according to Dionex Technical Note 53. Monosaccharide peak identity and relative abundance were determined using monosaccharide standards (Dionex).
  • the nonfucosylated anti-CD 19 21 D4 antibody was also tested using a standard capillary isoelectric focusing kit assay (Beckman Coulter). The assay returned observed pi values of pH 8.45 for fucosylated 21D4, 8.44 and 8.21 for fucosylated 21D4a, and 8.52 and 8.30 for the nonfucosylated 21D4 antibodies.
  • ADCC antibody dependent cellular cytotoxicity
  • Nonfucosylated human Anti-CD 19 monoclonal antibody 21 D4 was prepared as described above.
  • Human effector cells were prepared from whole blood as follows. Human peripheral blood mononuclear cells were purified from heparinized whole blood by standard Ficoll-paque separation. The cells were resuspended in RPMI 1640 media containing 10% FBS (culture media) and 200 U/ml of human IL-2 and incubated overnight at 37°C. The following day, the cells were collected and washed once in culture media and resuspended at 2 x 10 7 cells/ml.
  • Target CD 19+ cells were incubated with BATDA reagent (Perkin Elmer, Wellesley, MA) at 2.5 ⁇ l BATDA per 1 x 10 6 target cells/mL in culture media supplemented with 2.5mM probenecid (assay media) for 20 minutes at 37° C.
  • BATDA reagent Perkin Elmer, Wellesley, MA
  • the target cells were washed four times in PBS with 2OmM HEPES and 2.5mM probenecid, spun down and brought to a final volume of 1x10 " cells/ml in assay media.
  • the CD 19+ cell line ARH-77 human B lymphoblast leukemia; ATCC Accession

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Organic Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Human monoclonal antibodies that specifically bind to CD19 with high affinity are disclosed The antibodies are capable of internalizing into CD19-expressιng cells or are capable of mediating antigen dependent cellular cytotoxicity Nucleic acid molecules encoding the antibodies, expression vectors, host cells and methods for expressing the antibodies are also provided Antibody-partner molecule conjugates, bispecific molecules and pharmaceutical compositions compnsing the antibodies are also provided Also provided are methods for detecting CD19, as well as methods for treating cancers, such as B cell malignancies, for example, non-Hodgkin's lymphoma, chronic lymphocytic leukemias, follicular lymphomas, diffuse large cell lymphomas ofB lineage, and multiple myelomas using an anti-CD 19 antibody

Description

HUMAN ANTIBODIES THAT BIND CD19 AND USES THEREOF
Related Applications
This application claims priority to U.S. Provisional Application Serial No. 60/869,904, filed on December 13, 2006, and U.S. Provisional Application Serial No. 60/991,700, filed on November 30, 2007, the contents of which are hereby incorporated herein by reference.
Background CD 19 is a 95 kDa membrane receptor that is expressed early in B cell differentiation and continues to be expressed until the B cells are triggered to terminally differentiate (Pezzutto et al., (1987) J Immunol. 138:2793; Tedder et al. (1994) Immunol Today iJ>:437). The CD 19 extracellular domain contains two C2-type immunoglobulin (IG)-like domains separated by a smaller potentially disulfi de-linked domain. The CD 19 cytoplasmic domain is structurally unique, but highly conserved between human, mouse, and guinea pig (Fujimoto et al., (1998) Semin Immunol. JjO:267). CD 19 is part of a protein complex found on the cell surface of B lymphocytes. The protein complex includes CD 19, CD21 (complement receptor, type 2), CD81 (TAPA-I), and CD225 (Leu- 13) (Fujimoto, supra). CD 19 is an important regulator of transmembrane signals in B cells. An increase or decrease in the cell surface density of CD 19 affects B cell development and function, resulting in diseases such as autoimmunity or hypogammaglobulinemia (Fujimoto, supra). The CD 19 complex potentiates the response of B cells to antigen in vivo through cross-linking of two separate signal transduction complexes found on B cell membranes. The two signal transduction complexes, associated with membrane IgM and CD 19, activate phospholipase C (PLC) by different mechanisms. CD 19 and B cell receptor cross-linking reduces the number of IgM molecules required to activate PLC (Fujimoto, supra; Ghetie, supra). Additionally, CD 19 functions as a specialized adapter protein for the amplification of Arc family kinases (Hasegawa et al. , (2001 ) J Immunol 167:3190). CD 19 binding has been shown to both enhance and inhibit B-cell activation and proliferation, depending on the amount of cross-linking that occurs (Tedder, supra). CD 19 is expressed on greater than 90% of B-cell lymphomas and has been predicted to affect growth of lymphomas in vitro and in vivo (Ghetie, supra). Antibodies generated to CD 19 have been murine antibodies. A disadvantage of using a murine antibody in treatment of human subjects is the human anti-mouse (HAMA) response on administration to the patient. Accordingly, the need exists for improved therapeutic antibodies against CD 19 which are more effective for treating and/or preventing diseases mediated by CD 19.
Summary
The present disclosure provides isolated monoclonal antibodies, in particular human monoclonal antibodies, that specifically bind to CD 19 and that exhibit numerous desirable properties. These properties include high affinity binding to human CDl 9, internalization by cells expressing CD 19, and/or the ability to mediate antigen depu-uci > cellular cytotoxicity. The antibodies of the invention can be used, for example, to detect CD 19 protein or to inhibit the growth of cells expressing CD 19, such as tumor cells that express CD 19. Also provided are methods for treating a variety CD 19 mediated diseases using the antibodies and compositions of this disclosure.
In one aspect, this disclosure pertains to an isolated monoclonal human
Figure imgf000004_0001
or an antigen binding portion thereof, wherein the antibody binds human CD 19 and exhibits at least one of the following properties: (a) binds to human CD 19 with a K0 of 1 x 10"7 M or less;
(b) binds to Raji and Daudi B-cell tumor cells.
(c) is internalized by CD19-expressing cells;
(d) exhibits antibody dependent cellular cytotoxicity (ADCC) against CD 19 expressing cells; and (e) inhibits growth of CD19-expressing cells in vivo when conjugated to a cytotoxin.
Preferably, the antibody exhibits at least two of properties (a), (b), (c), (d), and (e). More preferably, the antibody exhibits at least three of properties (a), (b), (c), (d), and (e). More preferably, the antibody exhibits four of properties (a), (b), (c), (d), and (e). Even, more preferably, the antibody exhibits all five of properties (a), (b), (c), (d),
.?- and (e). In another preferred embodiment, the antibody inhibits growth of CDl 9- expressing tumor cells in vivo when the antibody is conjugated to a cytotoxin.
In one embodiment, the antibody binds to human CD 19 with a Kp of 5 x 10~8 M or less, binds to human CD 19 with a KD of 2 x 10"8 M or less, binds to human CD 19 with a KD of 1 x 10"8 M or less, binds to human CD 19 with a KD of 5x 10~9 M or less, binds to human CD 19 with a KD of 4x10"9 M or less, binds to human CD 19 with a KQ of 3xlO"9 M or less, or binds to human CD 19 with a K0 of 2 x 10~9 M or less.
Preferably the antibody is a human antibody, although in alternative embodiments the antibody can be a murine antibody, a chimeric antibody or humanized antibody. In another aspect, the invention pertains to an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody cross-competes for binding to an epitope on human CD 19 which is recognized by a reference antibody, wherein the reference antibody comprises:
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 ; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 9; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 2; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 3; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 1 1 ; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 5; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14; or the reference antibody comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15.
In another aspect, this disclosure pertains to an isolated monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a heavy chain variable region that is the product of or derived from a human VH 5-51 gene, wherein the antibody specifically binds CD 19. This disclosure also provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a heavy chain variable region that is the product of or derived from a human VH 1-69 gene, wherein the antibody specifically binds CD 19. This disclosure still further provides an isolated human monoclonal antibody, or antigen binding portion thereof comprising a light chain variable region that is the product of or derived from a human Vκ Ll 8 gene, wherein the antibody specifically binds CD 19. This disclosure even further provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a light chain variable region that is the product of or derived from a human VK A27 gene, wherein the antibody specifically binds CD 19. This disclosure even further provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a light chain variable region that is the product of or derived from a human VK L15 gene, wherein the antibody specifically binds CD19.
In a preferred embodiment, this disclosure provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises (a) a heavy chain variable region of a human VH 5-51 or 1-69 gene; and (b) a light chain variable region of a human Vκ Ll 8, A27 or VK L 15; wherein the antibody specifically binds to CD 19.
In another aspect, this disclosure provides an isolated human monoclonal antibody, or antigen binding portion thereof, wherein the antibody comprises a heavy chain variable region that comprises CDRl, CDR2, and CDR3 sequences; and a light chain variable region that comprises CDRl, CDR2, and CDR3 sequences, wherein: (a) the heavy chain variable region CDR3 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36, and conservative modifications thereof; (b) the light chain variable region CDR3 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequence of SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58, and conservative modifications thereof; (c) the antibody binds to human CD 19 with a Kp of IxI(T7 M or less; and (d) binds to Raji and Daudi B-cell tumor cells.
Preferably, the heavy chain variable region CDR2 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, and conservative modifications thereof; and the light chain variable region CDR2 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50, and conservative modifications thereof. Preferably, the heavy chain variable region CDRl sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22, and conservative modifications thereof; and the light chain variable region CDRl sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43, and conservative modifications thereof. A preferred combination comprises: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 16;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 23;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 30;
(d) a light chain variable region CDRl comprising SEQ ID NO: 37;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 44; and (f) a light chain variable region CDR3 comprising SEQ ID NO: 51.
Another preferred combination comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 16;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 23;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 30; (d) a light chain variable region CDRl comprising SEQ ID NO: 37;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 44; and (f) a light chain variable region CDR.3 comprising SEQ ID NO: 52. Another preferred combination comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 17;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 24; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 31;
(d) a light chain variable region CDR l comprising SEQ ID NO: 38;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 45; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 53. Another preferred combination comprises: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 18;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 25;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 32;
(d) a light chain variable region CDRl comprising SEQ ID NO: 39;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 46; and (f) a light chain variable region CDR3 comprising SEQ ID NO: 54.
Another preferred combination comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 19;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 26;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 33; (d) a light chain variable region CDRl comprising SEQ ID NO: 40;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 47; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 55. Another preferred combination comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 20; (b) a heavy chain variable region CDR2 comprising SEQ ID NO: 27;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 34;
(d) a light chain variable region CDRl comprising SEQ ID NO: 41;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 48; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 56. Another preferred combination comprises:
(a) a heavy chain variable region CDR 1 comprising SEQ ID NO: 21 ; (b) a heavy chain variable region CDR2 comprising SEQ ID NO: 28;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 35;
(d) a light chain variable region CDRl comprising SEQ ID NO: 42;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 49; and (f) a light chain variable region CDR3 comprising SEQ ID NO: 57.
Another preferred combination comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 22;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 29;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 36; (d) a light chain variable region CDRl comprising SEQ ID NO: 43;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 50; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 58. Other preferred antibodies, or antigen binding portions thereof comprise
(a) a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6 and 7; and
(b) a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 9, 10, 1 1, 12, 13, 14 and 15; wherein the antibody specifically binds CD 19.
A preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8.
Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 ; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 9. Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 2; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10.
Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 3; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 1 1. Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12.
Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 5; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13.
Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14. Another preferred combination comprises: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15.
In another aspect of this disclosure, antibodies, or antigen-binding portion or fragments thereof, are provided that compete for binding to CD 19 with any of the aforementioned antibodies.
The antibodies of this disclosure can be, for example, full-length antibodies, for example of an IgGl or IgG4 isotype. Alternatively, the antibodies can be antibody fragments, such as Fab, Fab' or Fab'2 fragments, or single chain antibodies.
This disclosure also provides an immunoconjugate comprising an antibody of this disclosure, or antigen-binding portion thereof, linked to a therapeutic agent, such as a cytotoxin or a radioactive isotope.
In a particularly preferred embodiment, the invention provides an immunoconjugate comprising an antibody of this disclosure, or antigen-binding portion thereof, linked to a cytotoxin (for example, a cytotoxin described herein or in U.S. Pat. App. No. 60/882,461, filed on December 28, 2006 or U.S. Pat. App. No. 60/991,300, filed on November 30, 2007, which are hereby incorporated by reference in their entirety) (e.g., via a thiol linkage). For example, in various embodiments, the invention provides the following preferred immunoconjugates:
(i) an immunoconjugate comprising an antibody, or antigen-binding portion thereof, comprising: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8.
(b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID
NO: 9.
(c) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 2 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10; (d) a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 3 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 1 1;
(e) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12;
(f) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 5 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13;
(g) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6 and a light chain variable region comprising the amino acid sequence of SEQ ID
NO: 14; or
(h) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15, where the antibody or antigen binding portion thereof is linked to a cytotoxin;
(ii) an immunoconjugate comprising an antibody, or antigen-binding portion thereof, comprising:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 16;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 23; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 30;
(d) a light chain variable region CDRl comprising SEQ ID NO: 37; (e) a light chain variable region CDR2 comprising SEQ ID NO: 44; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 51; an antibody, or antigen-binding portion thereof, comprising:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 16; (b) a heavy chain variable region CDR2 comprising SEQ ID NO: 23;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 30;
(d) a light chain variable region CDRl comprising SEQ ID NO: 37;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 44; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 52; an antibody, or antigen-binding portion thereof, comprising:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 17;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 24;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 31 ;
(d) a light chain variable region CDRl comprising SEQ ID NO: 38; (e) a light chain variable region CDR2 comprising SEQ ID NO: 45; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 53; an antibody, or antigen-binding portion thereof, comprising:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 18;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 25; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 32;
(d) a light chain variable region CDRl comprising SEQ ID NO: 39;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 46; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 54; an antibody, or antigen-binding portion thereof, comprising: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 19;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 26;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 33;
(d) a light chain variable region CDRl comprising SEQ ID NO: 40;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 47; and (f) a light chain variable region CDR3 comprising SEQ ID NO: 55; an antibody, or antigen-binding portion thereof, comprising: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 20;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 27;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 34;
(d) a light chain variable region CDRl comprising SEQ ID NO: 41; (e) a light chain variable region CDR2 comprising SEQ ID NO: 48; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 56; an antibody, or antigen-binding portion thereof, comprising:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 21;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 28; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 35;
(d) a light chain variable region CDRl comprising SEQ ID NO: 42;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 49; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 57; or an antibody, or antigen-binding portion thereof, comprising: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 22;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 29;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 36;
(d) a light chain variable region CDRl comprising SEQ ID NO: 43;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 50; and (f) a light chain variable region CDR3 comprising SEQ ID NO: 58, linked to a cytotoxin; and
(iii) an immunoconjugate comprising an antibody, or antigen-binding portion thereof, that binds to the same epitope that is recognized by (e.g., cross-competes for binding to human CD 19 with) an antibody comprising: (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID
NO: 8.
(b) a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 9. (c) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 2 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10;
(d) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 3 and a light chain variable region comprising the amino acid sequence of SEQ ID
NO: 11 ;
(e) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12; (t) a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 5 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13;
(g) a heavy chain variable region comprising the amino acid sequence of SF Q ID NO: 6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14; or
(h) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15, linked to a cytotoxin. This disclosure also provides a bispecific molecule comprising an antibody, or antigen-binding portion or fragment thereof, of this disclosure, linked to a second functional moiety having a different binding specificity than said antibody, or antigen binding portion thereof.
Compositions comprising an antibody, or antigen-binding portion thereof, or immunoconjugate or bispecific molecule of this disclosure and a pharmaceutically acceptable carrier are also provided.
Nucleic acid molecules encoding the antibodies, or antigen-binding portions thereof, of this disclosure are also encompassed by this disclosure, as well as expression vectors comprising such nucleic acids and host cells comprising such expression vectors. Methods for preparing anti-CD 19 antibodies using the host cells comprising such expression vectors are also provided and may include the steps of (i) expressing the antibody in the host cell and (ii) isolating the antibody from the host cell.
In yet another aspect, the invention pertains to a method for preparing an anti- CD 19 antibody. The method comprises: (a) providing: (i) a heavy chain variable region antibody sequence comprising a
CDRl sequence selected from the group consisting of SEQ ID NOs: 16-22, a CDR2 sequence selected from the group consisting of SEQ ID NOs:23-29, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs:30-36; and/or (ii) a light chain variable region antibody sequence comprising a CDRl sequence selected from the group consisting of SEQ ID NOs:37-43, a CDR2 sequence selected from the group consisting of SEQ ID NOs:44-50, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs:51-58;
(b) altering at least one amino acid residue within the heavy chain variable region antibody sequence and/or the light chain variable region antibody sequence to create at least one altered antibody sequence; and
(c) expressing the altered antibody sequence as a protein.
The present disclosure also provides isolated anti-CD 19 antibody-partner molecule conjugates that specifically bind to CD 19 with high affinity, particularly those comprising human monoclonal antibodies. Certain of such antibody-partner molecule conjugates are capable of being internalized into CD19-expressing cells and are capable of mediating antigen dependent cellular cytotoxicity. This disclosure also provides methods for treating cancers, such as treat B cell malignancies, including non-Hodgkin's lymphoma, chronic lymphocytic leukemias, follicular lymphomas, diffuse large cell lymphomas of B lineage, and multiple myelomas, using an anti-CD 19 antibody-partner molecule conjugate disclosed herein.
Compositions comprising an antibody, or antigen-binding portion thereof, conjugated to a partner molecule of this disclosure are also provided. Partner molecules that can be advantageously conjugated to an antibody in an antibody partner molecule conjugate as disclosed herein include, but are not limited to, molecules as drugs, cytotoxins, marker molecules (e.g., radioisotopes), proteins and therapeutic agents. Compositions comprising antibody-partner molecule conjugates and pharmaceutically acceptable earners are also disclosed herein.
In one aspect, such antibody-partner molecule conjugates are conjugated via chemical linkers. In some embodiments, the linker is a peptidyl linker, and is depicted herein as (L4)p — F — (L1 ),„. Other linkers include hydrazine and disulfide linkers, and is depicted herein as (L4)p — H — (L')m or (L4)p — J — (L')m , respectively. In addition to the linkers as being attached to the partner, the present invention also provides cleavable linker arms that are appropriate for attachment to essentially any molecular species.
In another aspect, the invention pertains to a method of inhibiting growth of a CD 19-expressing tumor cell. The method comprises contacting the CD 19-expressing tumor cell with an antibody-partner molecule conjugate of the disclosure such that growth of the CDl 9-expressing tumor cell is inhibited. In a preferred embodiment, the partner molecule is a therapeutic agent, such as a cytotoxin. Particularly preferred CD 19- expressing tumor cells are B-cell tumor cells. In another aspect, the invention pertains to a method of treating cancer in a subject. The method comprises administering to the subject an antibody-partner molecule conjugate of the disclosure such that the cancer is treated in the subject. In a preferred embodiment, the partner molecule is a therapeutic agent, such as a cytotoxin. Particularly preferred cancers for treatment are B cell malignancies, for example, non- Hodgkin's lymphoma, chronic lymphocytic leukemias, follicular lymphomas, diffuse large cell lymphomas of B lineage, and multiple myelomas.
Other features and advantages of the instant disclosure will be apparent from the following detailed description and examples which should not be construed as limiting. The contents of all references, Genbank entries, patents and published patent applications cited throughout this application are expressly incorporated herein by reference.
Brief Description of the Drawings
Figure IA shows the nucleotide sequence (SEQ ID NO: 59) and amino acid sequence (SEQ ID NO: 1) of the heavy chain variable region of the 21D4 and 21 D4a human monoclonal antibodies. The CDRl (SEQ ID NO: 16), CDR2 (SEQ ID NO: 23) and CDR3 (SEQ ID NO: 30) regions are delineated and the V, D and J germline derivations are indicated.
Figure IB shows the nucleotide sequence (SEQ ID NO: 66) and amino acid sequence (SEQ ID NO: 8) of the light chain variable region of the 21D4 human monoclonal antibody. The CDRl (SEQ ID NO: 37), CDR2 (SEQ ID NO: 44) and CDR3 (SEQ ID NO: 51) regions are delineated and the V and J germline derivations are indicated.
Figure 1 C shows the nucleotide sequence (SEQ ID NO: 67) and amino acid sequence (SEQ ID NO: 9) of the light chain variable region of the 21D4a human monoclonal antibody. The CDRl (SEQ ID NO: 37), CDR2 (SEQ ID NO: 44) and CDR3 (SEQ ID NO: 52) regions are delineated and the V and J germline derivations are indicated.
Figure 2A shows the nucleotide sequence (SEQ ID NO: 60) and amino acid sequence (SEQ ID NO: 2) of the heavy chain variable region of the 47G4 human monoclonal antibody. The CDRl (SEQ ID NO: 17), CDR2 (SEQ ID NO: 24) and CDR3 (SEQ ID NO: 31) regions are delineated and the V, D and J germline derivations are indicated.
Figure 2B shows the nucleotide sequence (SEQ ID NO: 68) and amino acid sequence (SEQ ID NO: 10) of the light chain variable region of the 47G4 human monoclonal antibody. The CDRl (SEQ ID NO: 38), CDR2 (SEQ ID NO: 45) and CDR3 (SEQ ID NO: 53) regions are delineated and the V and J germline derivations are indicated.
Figure 3 A shows the nucleotide sequence (SEQ ID NO: 61) and amino acid sequence (SEQ ID NO: 3) of the heavy chain variable region of the 27F3 human monoclonal antibody. The CDRl (SEQ ID NO: 18), CDR2 (SEQ ID NO: 25) and CDR3 (SEQ ID NO: 32) regions are delineated and the V, D and J germline derivations are indicated.
Figure 3B shows the nucleotide sequence (SEQ ID NO: 69) and amino acid sequence (SEQ ID NO: 11) of the light chain variable region of the 27F3 human monoclonal antibody. The CDRl (SEQ ID NO: 39), CDR2 (SEQ ID NO: 46) and CDR3 (SEQ ID NO: 54) regions are delineated and the V and J germline derivations are indicated.
Figure 4A shows the nucleotide sequence (SEQ ID NO: 62) and amino acid sequence (SEQ ID NO: 4) of the heavy chain variable region of the 3C10 human monoclonal antibody. The CDRl (SEQ ID NO: 19), CDR2 (SEQ ID NO: 26) and CDR3 (SEQ ID NO: 33) regions are delineated and the V, D and J germline derivations are indicated.
Figure 4B shows the nucleotide sequence (SEQ ID NO: 70) and amino acid sequence (SEQ ID NO: 12) of the light chain variable region of the 3C10 human monoclonal antibody. The CDRl (SEQ ID NO: 40), CDR2 (SEQ ID NO: 47) and CDR3 (SEQ ID NO: 55) regions are delineated and the V and J germline derivations are indicated.
Figure 5A shows the nucleotide sequence (SEQ ID NO: 63) and amino acid sequence (SEQ ID NO: 5) of the heavy chain variable region of the 5G7 human monoclonal antibody. The CDRl (SEQ ID NO: 20), CDR2 (SEQ ID NO: 27) and CDR3 (SEQ ID NO: 34) regions are delineated and the V, D and J geπnline derivations are indicated.
Figure 5B shows the nucleotide sequence (SEQ ID NO: 71) and amino acid sequence (SEQ ID NO: 13) of the light chain variable region of the 5G7 human monoclonal antibody. The CDRl (SEQ ID NO: 41), CDR2 (SEQ ID NO: 48) and CDR3 (SEQ ID NO: 56) regions are delineated and the V and J geπnline derivations are indicated.
Figure 6A shows the nucleotide sequence (SEQ ID NO: 64) and amino acid sequence (SEQ ID NO: 6) of the heavy chain variable region of the 13Fl human monoclonal antibody. The CDRl (SEQ ID NO: 21), CDR2 (SEQ ID NO: 28) and CDR3 (SEQ ID NO: 35) regions are delineated and the V, D and J germline derivations are indicated.
Figure 6B shows the nucleotide sequence (SEQ ID NO: 72) and amino acid sequence (SEQ ID NO: 14) of the light chain variable region of the 13Fl human monoclonal antibody. The CDRl (SEQ ID NO: 42), CDR2 (SEQ ID NO: 49) and CDR3 (SEQ ID NO: 57) regions are delineated and the V and J germline derivations are indicated.
Figure 7 A shows the nucleotide sequence (SEQ ID NO: 65) and amino acid sequence (SEQ ID NO: 7) of the heavy chain variable region of the 46E8 human monoclonal antibody. The CDRl (SEQ ID NO: 22), CDR2 (SEQ ID NO: 29) and
CDR3 (SEQ ID NO: 36) regions are delineated and the V, D and J germiine derivations are indicated.
Figure 7B shows the nucleotide sequence (SEQ ID NO: 73) and amino acid sequence (SEQ ID NO: 15) of the light chain variable region of the 46E8 human monoclonal antibody. The CDRl (SEQ ID NO: 43), CDR2 (SEQ ID NO: 50) and
CDR3 (SEQ ID NO: 58) regions are delineated and the V and J geπnline derivations are indicated.
Figure 8 shows the alignment of the amino acid sequence of the heavy chain variable region of 21D4 (SEQ ID NO: 1) and 21D4a (SEQ ID NO: 1), with the human geπnline VH 5-51 amino acid sequence (SEQ ID NO: 74). The JH4b germline is disclosed as SEQ ID NO: 80.
Figure 9 shows the alignment of the amino acid sequence of the heavy chain variable region of 47G4 (SEQ ID NO: 2) with the human geπnline Vn 1-69 amino acid sequences (SEQ ID NO: 75). The JH5b germline is disclosed as SEQ ID NO: 81. Figure 10 shows the alignment of the amino acid sequence of the heavy chain variable region of 27F3 (SEQ ID NO: 3), with the human germline Vn 5-51 amino acid sequence (SEQ ID NO: 74). The JH6b geπnline is disclosed as SEQ ID NO: 82.
Figure 11 shows the alignment of the amino acid sequence of the heavy chain variable region of 3C10 (SEQ ID NO: 4) with the human germline VH 1-69 amino acid sequences (SEQ ID NO: 75). The JH6b germline is disclosed as SEQ ID NO: 82.
Figure 12 shows the alignment of the amino acid sequence of the heavy chain variable region of 5G7 (SEQ ID NO: 5), with the human geπnline VH 5-51 amino acid sequence (SEQ ID NO: 74). The JH6b geπnline is disclosed as SEQ ID NO: 83.
Figure 13 shows the alignment of the amino acid sequence of the heavy chain variable region of 13Fl (SEQ ID NO: 6), with the human germline VH 5-51 amino acid sequence (SEQ ID NO: 74). The JH6b geπnline is disclosed as SEQ ID NO: 82. Figure 14 shows the alignment of the amino acid sequence of the heavy chain variable region of 46E8 (SEQ ID NO: 7), with the human germline VH 5-51 amino acid sequence (SEQ ID NO: 74). The JH6b germline is disclosed as SEQ ID NO: 82.
Figure 15 shows the alignment of the amino acid sequence of the light chain variable region of 21D4 (SEQ ID NO: 8) with the human germline Vk L18 amino acid sequence (SEQ ID NO:76). The JK2 geπnline is disclosed as SEQ ID NO: 84.
Figure 16 shows the alignment of the amino acid sequence of the light chain variable region of 21D4a (SEQ ID NO: 9) with the human geπnline Vt- LlS amino acid sequence (SEQ ID NO:76). The JK3 germline is disclosed as SEQ ID NO: 85. Figure 17 shows the alignment of the amino acid sequence of the light chain variable region of 47G4 (SEQ ID NO: 10) with the human germline Vk A27 amino acid sequence (SEQ ID NO:77). The JK3 geπnline is disclosed as SEQ ID NO: 85.
Figure 18 shows the alignment of the amino acid sequence of the light chain variable region of 27F3 (SEQ ID NO: 1 1 ) with the human germline Vk L18 amino acid sequence (SEQ ID NO:76). The JK2 germline is disclosed as SEQ ID NO: 84.
Figure 19 shows the alignment of the amino acid sequence of the light chain variable region of 3C10 (SEQ ID NO: 12) with the human germline Vk L15 amino acid sequence (SEQ ID NO:78). The JK2 germline is disclosed as SEQ ID NO: 84.
Figure 20 shows the alignment of the amino acid sequence of the light chain variable region of 5G7 (SEQ ID NO: 13) with the human germline Vk Ll 8 amino acid sequence (SEQ ID NO:76). The JKl geπnline is disclosed as SEQ ID NO: 86.
Figure 21 shows the alignment of the amino acid sequence of the light chain variable region of 13Fl (SEQ ID NO: 14) with the human germline Vk Ll 8 amino acid sequence (SEQ ID NO:76). The JK2 geπnline is disclosed as SEQ ID NO: 87. Figure 22 shows the alignment of the amino acid sequence of the light chain variable region of 46E8 (SEQ ID NO: 15) with the human germline Vk Ll 8 amino acid sequence (SEQ ID NO:76). The JK2 germline is disclosed as SEQ ID NO: 87.
Figure 23 is a graph showing the results of experiments demonstrating that the human monoclonal antibody 47 G4, directed against human CD 19, specifically binds to human CD 19. Figure 24 A and B are graphs showing the results of experiments demonstrating that the human monoclonal antibodies against CD 19 compete for binding on Raji cells.
Figure 25A-D shows the results of flow cytometry experiments demonstrating that the human monoclonal antibodies 21 D4, 21 D4a, 47G4, 3Cl 0, 5G7 and 13F 1 , directed against human CD 19, binds the cell surface of B-cell tumor cell lines. (A) Flow cytometry of HuMAbs 21D4 and 47G4 on CHO cells transfected with human CD 19. (B) Flow cytometry of HuMAb 47G4 on Daudi B tumor cells. (C) Flow cytometry of HuMAbs 21 D4 and 47G4 on Raji B tumor cells. (D) Flow cytometry of HuMAbs 21 D4, 21D4a, 3C10, 5G7 and 13Fl on Raji B tumor cells. Figures 26A-B shows the results of internalization experiments demonstrating that the human monoclonal antibodies 21D4 and 47G4, directed against human CD 19, enters CHO-CD 19 and CD19-expressing Raji B tumor cells by a 3H-thymidine release assay. (A) HuMAb 47G4 internalization into CHO-CD 19 cells. (B) HuMAbs 21 D4 and 47G4 internalization into Raji B tumor cells. Figure 27A and B shows the results of a thymidine incorporation assay demonstrating that human monoclonal antibodies directed against human CD 19 kill Raji B cell tumor cells.
Figure 28 shows a Kaplan-Meier plot of mouse survival in a Ramos systemic model. Figure 29A-B shows the body weight change in mice in a Ramos systemic model.
Figure 30A-B shows the results of an in vivo mouse tumor model study demonstrating that treatment with naked anti-CD 19 antibody 21D4 has a direct inhibitory effect on lymphoma tumors in vivo. (A) ARH-77 tumors (B) Raji tumors.
Figure 31 shows the results of an antibody dependent cellular cytotoxicity (ADCC) assay demonstrating that nonfucosylated human monoclonal anti-CD 19 antibodies have increased cell cytotoxicity on human leukemia cells in an ADCC dependent manner.
Figure 32 shows the results of an in vivo mouse tumor model study demonstrating that cytotoxin-conjugated anti-CD19 antibodies reduce tumor volume. Toxin 1 is cytotoxin Nl and toxin 2 is cytotoxin N2. Figure 33 shows the body weight change in mice in a Raji tumor model study. Toxin 1 is cytotoxin Nl and toxin 2 is cytotoxin N2.
Figure 34 shows the results of a cynomolgus monkey study showing a decreased population of CD20+ cells following treatment of iucosylated or nonfucosylated anti- CD 19 HuMAbS.
Figure 35 shows the results of individual cynomolgus monkeys following treatment with fucosylated or nonfucosylated anti-CD 19 HuMAbs.
Figure 36A-C shows the results of a thymidine incoiporation assay demonstrating that human monoclonal antibodies directed against human CD 19 alone or cytotoxin- conjugated kill Raji and SU-DHL-6 B cell tumor cells.
Figure 37 shows the in vivo efficacy of immunoconjugate anti-CD 19-N2 against tumor formation in a subcutaneous xenograft SCID mouse model.
Figure 38 shows the in vivo efficacy of immunoconjugate anti-CD 19-N2 against tumor formation in a subcutaneous Burkitt's lymphoma SCID mouse model. Figure 39 shows the in vivo efficacy of immunoconjugate anti-CD 19-N2 against tumor formation in a systemic SCID mouse model.
Figure 4OA shows that B cells (CD20+) were decreased in a dose-dependent manner after administration of 21D4 with minimal or no depletion at 0.01 mg/kg. B cells decreased to 16% to 32% of baseline after administration of 0.1 mg/kg. Figure 4OB illustrates that the magnitude and length of B-cell depletion after administration of 21D4 was similar to that of a 0.1 mg/kg injection of rituximab.
Figure 41 shows the in vivo efficacy of a single dose of anti-CD 19-cytotoxin A against tumor formation in a Raji xenograft SCID mouse model.
Figure 42 shows the in vivo efficacy of a single dose of anti-CD 19-cytotoxin A against tumor formation in a Raji xenograft SClD mouse model, including an isotype control.
Figure 43 shows the in vivo efficacy of a single dose and repeat doses of anti- CD 19-cytotoxin A against tumor formation in a Ramos xenograft Es le nude mouse model. Figure 44 shows the in vivo efficacy of a single dose of anti-CD 19-cytotoxin A against tumor formation in a Daudi xenograft SCID mouse model. Figure 45 shows the in vivo efficacy of a single dose of anti-CD 19-N2 against tumor formation in a SU-DHL6 xenograft SClD mouse model. N2 = cytotoxin B. Figure 46 is the structure of cytotoxin A.
Detailed Description The present disclosure relates to isolated monoclonal antibodies, particularly human monoclonal antibodies which bind specifically to human' CD 19 with high affinity and that have desirable functional properties. In certain embodiments, the antibodies of this disclosure are derived from particular heavy and light chain germline sequences and/or comprise particular structural features such as CDR regions comprising particular amino acid sequences. This disclosure provides isolated antibodies, methods of making such antibodies, antibody-partner molecule conjugates, and bispecific molecules comprising such antibodies and pharmaceutical compositions containing the antibodies, antibody-partner molecule conjugates or bispecific molecules of this disclosure. This disclosure also relates to methods of using the antibodies, such as to detect CD 19, as well as to treat diseases associated with expression of CD19, such as B cell malignancies that express CD 19. Accordingly, this disclosure also provides methods of using the anti- CD 19 antibodies and antibody-partner molecule conjugates of this disclosure to treat B cell malignancies, for example, in the treatment of non-Hodgkin"s lymphoma, chronic lymphocytic leukemias, follicular lymphomas, diffuse large cell lymphomas of B lineage, and multiple myelomas.
In order that the present disclosure may be more readily understood, certain terms are first defined. Additional definitions are set forth throughout the detailed description.
As used herein, the term "CD 19" refers to, for example, variants, iso forms, homologs, orthologs and paralogs of human CD 19. Accordingly, human antibodies of this disclosure may, in certain cases, cross-react with CD 19 from species other than human. In certain embodiments, the antibodies may be completely specific for one or more human CD 19 proteins and may not exhibit species or other types of non-human cross-reactivity, or may cross-react with CD 19 from certain other species but not all other species (e.g., cross-react with a primate CD 19 but not mouse CD 19). The term "human CD 19"' refers to human sequence CD 19, such as the complete amino acid sequence of human CD 19 having Genbank Accession Number NM_001770 (SEQ ID NO: 79). The term "mouse CD 19" refers to mouse sequence CD 19, such as the complete amino acid sequence of mouse CD 19 having Genbank Accession Number AAA37390. The human CD 19 sequence may differ from human CD 19 of Genbank Accession Number NM_001770 by having, for example, conserved mutations or mutations in non- conserved regions and the CD 19 has substantially the same biological function as the human CD 19 of Genbank Accession Number NMJ301770.
A particular human CD 19 sequence will generally be at least 90% identical in amino acids sequence to human CD 19 of Genbank Accession Number NIvIJ)01770 and contains amino acid residues that identify the amino acid sequence as being human when compared to CD 19 amino acid sequences of other species (e.g., murine). In certain cases, a human CD 19 may be at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to CD 19 of Genbank Accession Number NM_001770. In certain embodiments, a human CD 19 sequence will display no more than 10 amino acid differences from the CD 19 sequence of Genbank Accession Number NM_001770. In certain embodiments, the human CD 19 may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the CD 19 sequence of Genbank Accession Number NMJ301770. Percent identity can be determined as described herein.
The term "immune response" refers to the action of, for example, lymphocytes, antigen presenting cells, phagocytic cells, granulocytes, and soluble macromolecules produced by the above cells or the liver (including antibodies, cytokines, and complement) that results in selective damage to, destruction of, or elimination from the human body of invading pathogens, cells or tissues infected with pathogens, cancerous cells, or, in cases of autoimmunity or pathological inflammation, normal human cells or tissues.
A "signal transduction pathway" refers to the biochemical relationship between a variety of signal transduction molecules that play a role in the transmission of a signal from one portion of a cell to another portion of a cell. As used herein, the phrase "cell surface receptor" includes, for example, molecules and complexes of molecules capable of receiving a signal and the transmission of such a signal across the plasma membrane of
-?">. a cell. An example of a "cell surface receptor" of the present disclosure is the CD 19 receptor.
The term "antibody'* as referred to herein includes whole antibodies and any antigen binding fragment (i.e., "antigen-binding portion") or single chains thereof. An "antibody" refers to a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds, or an antigen binding portion thereof. Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as VH) and a heavy chain constant region. The heavy chain constant region is comprised of three domains, CH I , CH2 and CH3. Each light chain is comprised of a light chain variable region (abbreviated herein as VL) and a light chain constant region. The light chain constant region is comprised of one domain, CL. The VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR). Each VH and VL is composed of three CDRs and four FRs, arranged from amino- terminus to carboxy-teπninus in the following order: FRl, CDRl, FR2, CDR2, FR3,
CDR3, FR4. The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen. The constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (CIq) of the classical complement system.
The term "antibody fragment'' and "antigen-binding portion" of an antibody (or simply "antibody portion"), as used herein, refer to one or more fragments of an antibody that retain the ability to specifically bind to an antigen (eg., CD 19). It has been shown that the antigen-binding function of an antibody can be performed by fragments of a full- length antibody. Examples of binding fragments encompassed within the term "antigen- binding portion" of an antibody include (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, C1 and CH I domains; (ii) a F(ab')2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fab1 fragment, which is essentially an Fab with part of the hinge region (see, FUNDAMENTAL IMMUNOLOGY, Paul ed., 3rd ed. 1993); (iv) a Fd fragment consisting of the VH and Ci | 1 domains; (v) a Fv fragment consisting of the VL and Vn
-21- domains of a single arm of an antibody, (vi) a dAb fragment (Ward et al., (1989) Nature 341:544-546), which consists of a Vn domain; (vii) an isolated complementarity determining region (CDR); and (viii) a nanobody, a heavy chain variable region containing a single variable domain and two constant domains. Furthermore, although the two domains of the Fv fragment, V1 and Vn, are coded for by separate genes, they can be joined, using recombinant methods, by a synthetic linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see e.g., Bird et al. (1988) Science 242:423- 426; and Huston et al. (1988) Proc. Natl. Acad. ScL USA 85:5879-5883). Such single chain antibodies are also intended to be encompassed within the term "antigen-binding portion" of an antibody. These antibody fragments are obtained using conventional techniques known to those with skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies.
An "isolated antibody", as used herein, is intended to refer to an antibody that is substantially free of other antibodies having different antigenic specificities (e.g., an isolated antibody that specifically binds CD 19 is substantially free of antibodies that specifically bind antigens other than CD 19). An isolated antibody that specifically binds CD 19 may, however, have cross-reactivity to other antigens, such as CD 19 molecules from other species. Moreover, an isolated antibody may be substantially free of other cellular material and/or chemicals.
The terms "monoclonal antibody" or "monoclonal antibody composition" as used herein refer to a preparation of antibody molecules of single molecular composition. A monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope. The term "human antibody", as used herein, is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from human germline immunoglobulin sequences. Furthermore, if the antibody contains a constant region, the constant region also is derived from human germline immunoglobulin sequences. The human antibodies of this disclosure may include amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo). However, the tenn "human antibody", as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences. The tenn "human monoclonal antibody" refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human germline immunoglobulin sequences. In one embodiment, the human monoclonal antibodies are produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell.
The term "recombinant human antibody", as used herein, includes all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as (a) antibodies isolated from an animal {e.g., a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom (described further below), (b) antibodies isolated from a host cell transformed to express the human antibody, e.g., from a transfectoma, (c) antibodies isolated from a recombinant, combinatorial human antibody library, and (d) antibodies prepared, expressed, created or isolated by any other means that involve splicing of human immunoglobulin gene sequences to other DNA sequences. Such recombinant human antibodies have variable regions in which the framework and CDR regions are derived from human germline immunoglobulin sequences. In certain embodiments, however, such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the VH and V( regions of the recombinant antibodies are sequences that, while derived from and related to human germline VH and Vi sequences, may not naturally exist within the human antibody germline repertoire in vivo.
As used herein, "isotype" refers to the antibody class (e.g., IgM or IgGl) that is encoded by the heavy chain constant region genes. The phrases "an antibody recognizing an antigen" and "an antibody specific for an antigen" are used interchangeably herein with the term "an antibody which binds specifically to an antigen."'
The term "human antibody derivatives" refers to any modified form of the human antibody, e.g., a conjugate of the antibody and another agent or antibody.
The term "humanized antibody" is intended to refer to antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences. Additional framework region modifications may be made within the human framework sequences. The term "chimeric antibody" is intended to refer to antibodies in which the variable region sequences are derived from one species and the constant region sequences are derived from another species, such as an antibody in which the variable region sequences are derived from a mouse antibody and the constant region sequences are derived from a human antibody. The teπn "antibody mimetic" is intended to refer to molecules capable of mimicking an antibody's ability to bind an antigen, but which are not limited to native antibody structures. Examples of such antibody mimetics include, but are not limited to, Affϊbodies, DARPins, Anticalins, Avimers, and Versabodies, all of which employ binding structures that, while they mimic traditional antibody binding, are generated from and function via distinct mechanisms.
As used herein, the term "partner molecule" refers to the entity which is conjugated to an antibody in an antibody-partner molecule conjugate. Examples of partner molecules include drags, cytotoxins, marker molecules (including, but not limited to peptide and small molecule markers such as fluorochrome markers, as well as single atom markers such as radioisotopes), proteins and therapeutic agents.
As used herein, an antibody that "specifically binds to human CD 19" is intended to refer to an antibody that binds to human CD 19 with a KD of I x 10"7 M or less, more preferably 5 x 1O*8 M or less, more preferably 3 x 10 s M or less, more preferably 1 x 1(T8 M or less, even more preferably 5 x 10"9 M or less. The term "does not substantially bind" to a protein or cells, as used herein, means does not bind or does not bind with a high affinity to the protein or cells, i.e. binds to the protein or cells with a Kp of 1 x 10"6 M or more, more preferably 1 x 10"5 M or more, more preferably 1 x 10" M or more, more preferably 1 x 10° M or more, even more preferably 1 x 1O"2 M or more.
The term "Kassoc" or "Ka", as used herein, is intended to refer to the association rate of a particular antibody-antigen interaction, whereas the term "Kdis" or "'Kd," as used herein, is intended to refer to the dissociation rate of a particular antibody- antigen interaction. The term "'KD", as used herein, is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e., Kd/Ka) and is expressed as a molar concentration (M). KD values for antibodies can be determined using methods well established in the art. A preferred method for determining the KQ of an antibody is by using surface plasmon resonance, preferably using a biosensor system such as a Biacore" system.
As used herein, the term ''high affinity" for an IgG antibody refers to an antibody having a Kp of 1 x 10"7 M or less, more preferably 5 x 10"8 M or less and even more preferably 1 x 10"9 M or less and even more preferably 5 x 10"9 M or less for a target antigen. However, ''high affinity" binding can vary for other antibody isotypes. For example, "high affinity" binding for an IgM isotype refers to an antibody having a KD of 10"6 M or less, more preferably 10"7 M or less, even more preferably 10 s M or less.
As used herein, the term "subject" includes any human or nonhuman animal. The term "nonhuman animal" includes all vertebrates, e.g., mammals and non-mammals, such as nonhuman primates, sheep, dogs, cats, horses, cows, chickens, amphibians, reptiles, etc.
The symbol *'-", whether utilized as a bond or displayed perpendicular to a bond, indicates the point at which the displayed moiety is attached to the remainder of the molecule, solid support, etc.
The term "alkyl," by itself or as part of another substituent, means, unless otherwise stated, a straight or branched chain, or cyclic hydrocarbon radical, or combination thereof, which may be fully saturated, mono- or polyunsaturated and can include di- and multivalent radicals, having the number of carbon atoms designated (i.e., C|-Cιo means one to ten carbons). Examples of saturated hydrocarbon radicals include, but are not limited to, groups such as methyl, ethyl, n-propyl, isopropyl. n-butyl, t-butyl, isobutyl, sec-butyl, cyclohexyl, (cyclohexyl)methyl, cyclopropylmethyl, homologs and isomers of, for example, n-pentyl, n-hexyl, n-heptyl, n-octyl, and the like. An unsaturated alkyl group is one having one or more double bonds or triple bonds. Examples of unsaturated alkyl groups include, but are not limited to, vinyl, 2-propenyl, crotyl, 2-isopentenyl, 2-(butadienyl), 2,4-pentadienyl, 3-(l,4-pentadienyl). ethynyl, 1- and 3-propynyl, 3-butynyl, and the higher homologs and isomers. The term "alkyl," unless otherwise noted, is also meant to include those derivatives of alkyl defined in more detail below, such as "heteroalkyl." Alkyl groups, which are limited to hydrocarbon groups are termed "homoalkyl". The term "alkylene" by itself or as part of another substituent means a divalent radical derived from an alkane, as exemplified, but not limited, by -CHiCH2CH2CH?-, and further includes those groups described below as "heteroalkylene." Typically, an alkyl (or alkylene) group will have from 1 to 24 carbon atoms, with those groups ha\ ing 10 or fewer carbon atoms being preferred in the present invention. A '"lower alkyl" or "lower alkylene" is a shorter chain alkyl or alkylene group, generally having eight or fewer carbon atoms.
The term "heteroalkyl," by itself or in combination with another term, means, unless otherwise stated, a stable straight or branched chain, or cyclic hydrocarbon radical, or combinations thereof, consisting of the stated number of carbon atoms and at least one heteroatom selected from the group consisting of O, N, Si, and S, and wherein the nitrogen, carbon and sulfur atoms may optionally be oxidized and the nitrogen heteroatom may optionally be quaternized. The heteroatom(s) O, N, S, and Si may be placed at any interior position of the heteroalkyl group or at the position at which the alkyl group is attached to the remainder of the molecule. Examples include, but are not limited to, -CH2-CH2-O-CH,, -CH2-CH2-NH-CH3, -CH2-CH2-N(CH3)~CH3, -CH2-S-CH2- CH3, -CH2-CH25-S(O)-CH5, -CH2-CH2-S(O)2-CH3,
Figure imgf000030_0001
-Si(CH3),, -CH2- CH=N-OCH3, and -CH=CH-N(CH3)-CH3. Up to two heteroatoms may be consecutive, such as, for example, -CH2-NH-OCH3 and -CH2-O-Si(CH3)3. Similarly, the term "heteroalkylene" by itself or as part of another substituent means a divalent radical derived from heteroalkyl, as exemplified, but not limited by, -CH2-CH2-S-CH2-CH2- and --CH2-S-CH2-CH2-NH-CH2-. For heteroalkylene groups, heteroatoms can also occupy either or both of the chain termini (e.g., alkyleneoxy, alkylenedioxy, alkyleneamino, alkylenediamino, and the like). The terms "heteroalkyl" and "heteroalkylene" encompass poly(ethylene glycol) and its derivatives (see, for example, Shearwater Polymers Catalog, 2001). Still further, for alkylene and heteroalkylene linking groups, no orientation of the linking group is implied by the direction in which the formula of the linking group is written. For example, the formula -C(O)2R*- represents both -C(O)2R'- and -R1C(O)2-. The term "lower" in combination with the terms "alkyl" or "heteroalkyl" refers to a moiety having from 1 to 6 carbon atoms.
The terms "aikoxy," "alkylamino," "alkylsulfonyl," and "alkylthio" (or thioalkoxy) are used in their conventional sense, and refer to those alkyl groups attached to the remainder of the molecule via an oxygen atom, an amino group, an SO2 group or a sulfur atom, respectively. The term "arylsulfonyl" refers to an aryl group attached to the remainder of the molecule via an SO2 group, and the term "sulfhydryl" refers to an SH group. In general, an "acyl substituent" is also selected from the group set forth above.
As used herein, the term "acyl substituent" refers to groups attached to, and fulfilling the valence of a carbonyl carbon that is either directly or indirectly attached to the polycyclic nucleus of the compounds of the present invention.
The terms "cycloalkyl" and "heterocycloalkyl", by themselves or in combination with other terms, represent, unless otherwise stated, cyclic versions of substituted or unsubstituted "alkyl" and substituted or unsubstituted "heteroalkyl", respectively. Additionally, for heterocycloalkyl, a heteroatom can occupy the position at which the heterocycle is attached to the remainder of the molecule. Examples of cycloalkyl include, but are not limited to, cyclopentyl, cyclohexyl, 1-cyclohexenyl, 3-cyclohexenyl, cycloheptyl, and the like. Examples of heterocycloalkyl include, but are not limited to, 1 -(1,2,5,6-tetrahydropyridyl), 1-piperidinyl, 2-piperidinyl, 3-piperidinyl, 4-morpholinyl, 3-morpholinyl, tetrahydrofuran-2-yl, tetrahydrofuran-3-yl, tetrahydrothien-2-yl, tetrahydrothien-3-yl, 1 -piperazinyl, 2-piperazinyl, and the like. The heteroatoms and carbon atoms of the cyclic structures are optionally oxidized. The terms "halo" or "halogen," by themselves or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom. Additionally, terms such as "haloalkyl," are meant to include monohaloalkyl and polyhaloalkyl. For example, the term "'halo(Ci-C4)alkyr is meant to include, but not be limited to, trifluoromethyl, 2,2,2-trifluorσethyl, 4-chlorobutyl, 3-bromopropyl, and the like. The teπn "aryl" means, unless otherwise stated, a substituted or unsubstituted polyunsaturated, aromatic, hydrocarbon substituent which can be a single ring or multiple rings (preferably from 1 to 3 rings) which are fused together or linked covalently. The term "heteroaryl" refers to aryl groups (or rings) that contain from one to four heteroatoms selected from N, O, and S, wherein the nitrogen, carbon and sulfur atoms are optionally oxidized, and the nitrogen atom(s) are optionally quatemized. A heteroaryl group can be attached to the remainder of the molecule through a heteroatom. Non- limiting examples of aryl and heteroaryl groups include phenyl, 1-naphthyl, 2-naphthyl, 4-biphenyl, 1-pyrrolyl, 2-pyrrolyl, 3-pyrrolyl, 3-pyrazolyl, 2-imidazolyl, 4-imidazolyl, pyrazinyl, 2-oxazolyl, 4-oxazolyl, 2-phenyl-4-oxazolyl, 5-oxazolyl, 3-isoxazolyl, A- isoxazolyl, 5-isoxazolyl, 2-thiazolyl, 4-thiazolyl, 5-thiazolyl, 2-furyl, 3-furyl, 2-thienyl, 3-thienyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, 2-pyrimidyl, 4-pyrimidyl, 5-benzothiazolyl, purinyl, 2-benzimidazolyl, 5-indolyl, 1-isoquinolyl, 5-isoquinolyl, 2-quinoxalinyl, 5- quinoxalinyl, 3-quinolyl, and 6-quinolyl. Substituents for each of the above noted aryl and heteroaryl ring systems are selected from the group of acceptable substituents described below. "Aryl" and "heteroaryl" also encompass ring systems in which one or more non-aromatic ring systems are fused, or otherwise bound, to an aryl or heteroaryl system.
For brevity, the term "aryF" when used in combination with other terms (e.g., aryloxy, arylthioxy, arylalkyl) includes both aryl and heteroaryl rings as defined above. Thus, the term ''arylalkyP is meant to include those radicals in which an aryl group is attached to an alkyl group (e.g., benzyl, phenethyl, pyridylmethyl and the like) including those alkyl groups in which a carbon atom (e.g., a methylene group) has been replaced by, for example, an oxygen atom (e.g., phenoxymethyl, 2-pyridyloxymethyl, 3-(l- naphthyloxy)propyl, and the like). Each of the above terms (e.g., "alkyl," "heteroalkyl," "aryl" and "hetetOaryl") include both substituted and unsubstituted forms of the indicated radical. Preferred substituents for each type of radical are provided below.
Substituents for the alkyl, and heteroalkyl radicals (including those groups often referred to as alkylene, alkenyl, heteroalkyl ene, heteroalkenyl, alkynyl, cycloalkyl, heterocycloalkyl, cycloalkenyl, and heterocycloalkenyl) are generally referred to as "alkyl substituents" and "heteroalkyl substituents,'' respectively, and they can be one or more of a variety of groups selected from, but not limited to: -OR', =O, =NR", =N-OR", - NR'R", -SR', -halogen, -SiR'R"R"\ -OC(O)R', -C(O)R', -CO2R', -CONR'R", - OC(O)NR5R", -NR"C(0)R', -NR'-C(0)NR"R"\ -NR55C(O)2R5, -NR-
C(NR5R"R'")=NR"", -NR-C(NR5Rl-NR'", -S(O)R*, -S(O)2R', -S(O)2NR5R", -NRSO2R', -CN and -NO2 in a number ranging from zero to (2m"+l), where m' is the total number of carbon atoms in such radical. R', R", R"'* and R"" each preferably independently refer to hydrogen, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, e.g., aryl substituted with 1-3 halogens, substituted or unsubstituted alkyl, alkoxy or thioalkoxy groups, or arylalkyl groups. When a compound of the invention includes more than one R group, for example, each of the R groups is independently selected as are each R\ R", R1" and R'"' groups when more than one of these groups is present. When R' and R" are attached to the same nitrogen atom, they can be combined with the nitrogen atom to form a 5, 6, or 7-membered ring. For example, -NR'R" is meant to include, but not be limited to, l~pyrrolidinyl and 4- morpholinyl. From the above discussion of substituents, one of skill in the art will understand that the term "alkyl" is meant to include groups including carbon atoms bound to groups other than hydrogen groups, such as haloalkyl (e.g., -CF3 and -CH2CF3) and acyl (e.g., -C(O)CH3, -C(O)CF3, -C(O)CH2OCH3, and the like).
Similar to the substituents described for the alkyl radical, the aryl substituents and heteroaryl substituents are generally referred to as "aryl substituents" and "heteroaryl substituents," respectively and are varied and selected from, for example: halogen, -OR', =O, -NR', =N-0R\ -NR'R", -SR', -halogen, -SiR5R5-R"5, -OC(O)R', -C(O)R', -CO2R', -CONR'R", -0C(0)NR'R", -NR"C(O)R\ -NR"-C(0)NR"R"\ -NR55C(O)2R'.
-NR-C(NR"R")=NR'",.-S(O)R\ -S(O)2R5, -S(O)2NR5R", -NRSO2R', -CN and -NO2, - R', -Nj, -CH(Ph)O, fluoro(CrC4)alkoxy, and fluoro(C|-C4)alkyl, in a number ranging from zero to the total number of open valences on the aromatic ring system; and where R', R", R"' and R"" are preferably independently selected from hydrogen, (CrC8)alkyl and heteroalkyl, unsubstituted aryl and heteroaryl, (unsubstituted aryl)-(C|-C4)alkyl, and (unsubstituted aryl)oxy-(Cj-C4)alkyl. When a compound of the invention includes more than one R group, for example, each of the R groups is independently selected as are each R', R'', R'" and R"" groups when more than one of these groups is present.
Two of the aryl substituents on adjacent atoms of the aryl or heteroaryl ring may optionally be replaced with a substituent of the formula -T-C(O)-(CRR' )q- U-, wherein T and U are independently -NR-, -O-, -CRR'- or a single bond, and q is an integer of from 0 to 3. Alternatively, two of the substituents on adjacent atoms of the aryl or heteroaryl ring may optionally be replaced with a substituent of the formula -A-(CHo)1-B-, wherein A and B are independently -CRR'-, -O-, -NR-, -S-, -S(O)-, -S(O)2-, -S(O)2NR1- or a single bond, and r is an integer of from 1 to 4. One of the single bonds of the new ring so formed may optionally be replaced with a double bond. Alternatively, two of the substituents on adjacent atoms of the aryl or heteroaryl ring may optionally be replaced with a substituent of the formula -(CRR')s-X-(CR"R"")d-, where s and d are independently integers of from 0 to 3, and X is -O-, -NR'-, -S-, -S(O)-, -S(O)2-, or -- S(O)2NR'-. The substituents R, R', R" and R"" are preferably independently selected from hydrogen or substituted or unsubstituted (Ci-C6) alkyl.
As used herein, the term "diphosphate" includes but is not limited to an ester of phosphoric acid containing two phosphate groups. The term "triphosphate" includes but is not limited to an ester of phosphoric acid containing three phosphate groups. For example, particular drugs having a diphosphate or a triphosphate include:
Figure imgf000034_0001
As used herein, the term "heteroatom" includes oxygen (O), nitrogen (N), sulfur (S) and silicon (Si).
The symbol "R" is a general abbreviation that represents a substituent group that is selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocyclyl groups.
Various aspects of the invention are described in further detail in the following subsections.
Anti-CD 19 Antibodies Having Particular Functional Properties
The antibodies of this disclosure are characterized by particular functional features or properties of the antibodies. For example, the antibodies specifically bind to human CD 19. Preferably, an antibody of this disclosure binds to CD 19 with high affinity, for example with a KD of 1 x 10" M or less. The anti-CD 19 antibodies of this disclosure preferably exhibit one or more of the following characteristics:
(a) binds to human CD 19 with a KD of 1x107 M or less;
(b) binds to Raji and Daudi B-cell tumor cells.
(c) is internalized by CD 19-expressing cells;
(d) exhibits antibody dependent cellular cytotoxicity (ADCC) against CD 19 expressing cells; and
(e) inhibits growth of CD 19-expressing cells in vivo when conjugated to a cytotoxin.
Preferably, the antibody exhibits at least two of properties (a), (b), (c), (d), and (e). More preferably, the antibody exhibits at least three of properties (a), (b), (c), (d), and (e). More preferably, the antibody exhibits four of properties (a), (b), (c), (d), and (e). Even, more preferably, the antibody exhibits all five of properties (a), (b), (c), (d), and (e). In another preferred embodiment, the antibody inhibits growth of CD 19- expressing tumor cells in vivo when the antibody is conjugated to a cytotoxin.
Preferably, the antibody binds to human CD 19 with a KD of 5 x 10 s M or less, binds to human CD 19 with a K0 of 1 x 10"8 M or less, binds to human CD 19 with a KD of 5 x 10"9 M or less, binds to human CD 19 with a KD of 4 X 10"9 M or less, binds to human CD 19 with a KD of 3 x 10"9 M or less, or binds to human CD 19 with a Kp of 2 x 10"9 M or less, or binds to human CD 19 with a KD of 1 x lθ"ή M or less.
The binding of an antibody of the invention to CD 19 can be assessed using one or more techniques well established in the art. For example, in a preferred embodiment, an antibody can be tested by a flow cytometry assay in which the antibody is reacted with a cell line that expresses human CD 19, such as CHO cells that have been transfected to express CD 19 on their cell surface or CD19-expressing cell lines such as OVCAR3, NCI- H226, CFPAC-I and/or KB (see, e.g., Example 3 A for a suitable assay and further description of cell lines).. Additionally or alternatively, the binding of the antibody, including the binding kinetics (e.g., KD value) can be tested in BIAcore binding assays (see , e.g., Example 3B for suitable assays). Still other suitable binding assays include ELISA assays, for example using a recombinant CD 19 protein see, e.g., Example 1 for a suitable assay).
Preferably, an antibody of this disclosure binds to a CD 19 protein with a KD of 5 x 10-8 M or less, binds to a CD 19 protein with a KD of 3 x 10-8 M or less, binds to a
CD 19 protein with a KD of 1 x 10-8 M or less, binds to a CD 19 protein with a KD of 7 x 10-9 M or less, binds to a CD 19 protein with a KD of 6 x 10-9 M or less or binds to a CD 19 protein with a KD of 5 x 10-9 M or less. The binding affinity of the antibody for CD 19 can be evaluated, for example, by standard BIACORE analysis, (see e.g., Example 3B).
Standard assays for evaluating internalization of anti-CD 19 antibodies by CD 19- expressing cells are known in the art (see e.g., the Hum-ZAP and immunofluorescence assays described in Example 5). Standard assays for evaluating binding of CD 19 to CAl 25, and inhibition thereof by anti-CD 19 antibodies, also are known in the art (see e.g., the OVCAR3 cell adhesion assay described in Example 6). Standard assays for evaluating ADCC against CD19-expressing cells also are known in the art (see e.g., the ADCC assay described in Example 7). Standard assays for evaluating inhibition of tumor cell growth in vivo by anti-CD 19 antibodies, and cytotoxin conjugates thereof, also are known in the art (see e.g., the tumor xenograft mouse models described in Example 8). Preferred antibodies of the invention are human monoclonal antibodies. Additionally or alternatively, the antibodies can be, for example, chimeric or humanized monoclonal antibodies.
Monoclonal Antibodies 21 D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8
Preferred antibodies of this disclosure are the human monoclonal antibodies 21D4, 2lD4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8, isolated and structurally characterized as described in Examples 16, 17, 18, 19, 20, 21 and 22. The Vn amino acid sequences of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 1, 1, 2, 3, 4, 5, 6 and 7, respectively. The VL amino acid sequences of 21D4,
21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 8, 9, 10, 1 1, 12, 13, 14 and 15, respectively.
Given that each of these antibodies can bind to CD 19, the VH and V|, sequences can be "mixed and matched" to create other anti-CD 19 binding molecules of this disclosure. CD 19 binding of such "mixed and matched" antibodies can be tested using the binding assays described above and in the Examples (e.g., ELISAs). Preferably, when VH and VL chains are mixed and matched, a VH sequence from a particular Vn/Vι pairing is replaced with a structurally similar VH sequence. Likewise, preferably a V1 sequence from a particular V5 /VL pairing is replaced with a structurally similar VL sequence.
Accordingly, in one aspect, this disclosure provides an isolated monoclonal antibody, or antigen binding portion thereof comprising:
(a) a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6 and 7; and (b) a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 9, 10, 1 1, 12, 13, 14 and 15; wherein the antibody specifically binds CD 19, preferably human CD 19. Preferred heavy and light chain combinations include:
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 ; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8; or (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 ; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 9; or
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 2; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10; or
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 3; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 1 1; or (a) a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 4; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12; or
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 5; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13; or
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14; or
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7; and (b) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15.
In another aspect, this disclosure provides antibodies that comprise the heavy chain and light chain CDRIs, CDR2s and CDR3s of 21D4, 21D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8, or combinations thereof. The amino acid sequences of the VH CDRIs of 21D4, 21D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22. The amino acid sequences of the VH CDR2s of 21D4, 21D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29. The amino acid sequences of the Vn CDR3s of 21D4, 21D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36. The amino acid sequences of the Vk CDRIs of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43. The amino acid sequences of the Vk CDR2s of 21 D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50. The amino acid sequences of the Vk CDR3s of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58. The CDR regions are delineated using the Kabat system (Kabat, E. A., et ai (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242).
Given that each of these antibodies can bind to CD 19 and that antigen-binding specificity is provided primarily by the CDRl, CDR2, and CDR3 regions, the Vn CDRl, CDR2, and CDR3 sequences and Vk CDRl, CDR2, and CDR3 sequences can be "mixed and matched" {i.e., CDRs from different antibodies can be mixed and match, although each antibody must contain a VH CDRl, CDR2, and CDR3 and a Vk CDRl, CDR2, and CDR3) to create other anti-CD 19 binding molecules of this disclosure. CD 19 binding of such "mixed and matched" antibodies can be tested using the binding assays described above and in the Examples {e.g., ELISAs, Biacore* analysis). Preferably, when VH CDR sequences are mixed and matched, the CDR 1 , CDR2 and/or CDR3 sequence from a particular VH sequence is replaced with a structurally similar CDR sequence(s). Likewise, when Vk CDR sequences are mixed and matched, the CDRl, CDR2 and/or CDR3 sequence from a particular V^ sequence preferably is replaced with a structurally similar CDR sequence(s). It will be readily apparent to the ordinarily skilled artisan that novel VH and VL sequences can be created by substituting one or more VH and/or V|, CDR region sequences with structurally similar sequences from the CDR sequences disclosed herein for monoclonal antibodies 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8. Accordingly, in another aspect, this disclosure provides an isolated monoclonal antibody, or antigen binding portion thereof comprising:
(a) a heavy chain variable region CDRl comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22;
(b) a heavy chain variable region CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29; (c) a heavy chain variable region CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36;
(d) a light chain variable region CDRl comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43; (e) a light chain variable region CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50; and
(f) a light chain variable region CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58; wherein the antibody specifically binds CD 19, preferably human CD 19. In a preferred embodiment, the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 16;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 23;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 30;
(d) a light chain variable region CDRI comprising SEQ ID NO: 37; (e) a light chain variable region CDR2 comprising SEQ ID NO: 44; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 51. In another preferred embodiment, the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 16;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 23; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 30;
(d) a light chain variable region CDRl comprising SEQ ID NO: 37;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 44; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 52. In another preferred embodiment, the antibody comprises: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 17;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 24;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 31 ;
(d) a light chain variable region CDRl comprising SEQ ID NO: 38;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 45; and (f) a light chain variable region CDR3 comprising SEQ ID NO: 53.
In another preferred embodiment, the antibody comprises: (a) a heavy chain variable region CDR 1 comprising SEQ ID NO: 18;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 25;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 32;
(d) a light chain variable region CDRl comprising SEQ ID NO: 39; (e) a light chain variable region CDR2 comprising SEQ ID NO: 46; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 54. In another preferred embodiment, the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 19;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 26; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 33;
(d) a light chain variable region CDRl comprising SEQ ID NO: 40;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 47; and (1) a light chain variable region CDR3 comprising SEQ ID NO: 55.
In another preferred embodiment, the antibody comprises: (a) a heavy chain variable region CDR 1 comprising SEQ ID NO: 20;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 27;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 34;
(d) a light chain variable region CDRl comprising SEQ ID NO: 41 ;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 48; and (t) a light chain variable region CDR3 comprising SEQ ID NO: 56.
In another preferred embodiment, the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 21;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 28;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 35; (d) a light chain variable region CDRl comprising SEQ ID NO: 42;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 49; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 57. In another preferred embodiment, the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 22; (b) a heavy chain variable region CDR2 comprising SEQ ID NO: 29;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 36; (d) a light chain variable region CDRl comprising SEQ ID NO: 43;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 50; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 58.
It is well known in the art that the CDR3 domain, independently from the CDRl and/or CDR2 domain(s), alone can determine the binding specificity of an antibody for a cognate antigen and that multiple antibodies can predictably be generated having the same binding specificity based on a common CDR3 sequence. See, for example, Klimka et al., British J. of Cancer 83(2):252-260 (2000) (describing the production of a humanized anti-CD30 antibody using only the heavy chain variable domain CDR3 of murine anti-CD30 antibody Ki-4); Beiboer et al, J. MoL Biol. 296:833-849 (2000) (describing recombinant epithelial glycoprotein-2 (EGP-2) antibodies using only the heavy chain CDR3 sequence of the parental murine MOC-31 anti-EGP-2 antibody); Rader et al, Proc. Natl. Acad. ScL U.S.A. 95:8910-8915 (1998) (describing a panel of humanized anti-integrin αvβ} antibodies using a heavy and light chain variable CDR3 domain of a murine anti-integrin αvp3 antibody LM609 wherein each member antibody comprises a distinct sequence outside the CDR3 domain and capable of binding the same epitope as the parent murine antibody with affinities as high or higher than the parent murine antibody); Barbas et al, J. Am. Chem. Soc. 116:2161-2162 (1994) (disclosing that the CDR3 domain provides the most significant contribution to antigen binding); Barbas et al., Proc. Natl. Acad. ScL U.S.A. 92:2529-2533 (1995) (describing the grafting of heavy chain CDR3 sequences of three Fabs (SI-I, SI-40, and SI-32) against human placental DNA onto the heavy chain of an anti-tetanus toxoid Fab thereby replacing the existing heavy chain CDR3 and demonstrating that the CDR3 domain alone conferred binding specificity); and Ditzel et al, J. Immunol. 157:739-749 (1996) (describing grafting studies wherein transfer of only the heavy chain CDR3 of a parent polyspecific Fab LNA3 to a heavy chain of a monospecific IgG tetanus toxoid-binding Fab p313 antibody was sufficient to retain binding specificity of the parent Fab); Berezov et al, BIAjournal 8:Scientific Review 8 (2001) (describing peptide mimetics based on the CDR3 of an anti-HER2 monoclonal antibody); Igarashi et al, J. Biochem (Tokyo) 117:452-7 (1995) (describing a 12 amino acid synthetic polypeptide corresponding to the CDR3 domain of an anti-phosphatidylserine antibody); Bourgeois et al, J. Virol 72:807- 10 (1998) (showing that a single peptide derived from the heavy chain CDR3 domain of an anti-respiratory syncytial virus (RSV) antibody was capable of neutralizing the virus in vitro); Levi et uL, Proc. Natl. Acad. Sci. U.S.A. 90:4374-8 (1993) (describing a peptide based on the heavy chain CDR3 domain of a murine anti-HIV antibody); Polymenis and Stoller, J. Immunol. 152:5218-5329 ( 1994) (describing enabling binding of an scFv by grafting the heavy chain CDR3 region of a Z-DNA-binding antibody); and Xu and Davis, Immunity 13:37-45 (2000) (describing that diversity at the heavy chain CDR3 is sufficient to permit otherwise identical IgM molecules to distinguish between a variety of hapten and protein antigens). See also, U.S. Patents Nos. 6,951,646; 6,914,128; 6,090,382; 6,818,216; 6,156,313; 6,827,925; 5,833,943; 5,762,905 and 5,760,185, describing patented antibodies defined by a single CDR domain. Each of these references is hereby incorporated by reference in its entirety.
Accordingly, the present disclosure provides monoclonal antibodies comprising one or more heavy and/or light chain CDR3 domains from an antibody derived from a human or non-human animal, wherein the monoclonal antibody is capable of specifically binding to CD 19. Within certain aspects, the present disclosure provides monoclonal antibodies comprising one or more heavy and/or light chain CDR3 domain from a non- human antibody, such as a mouse or rat antibody, wherein the monoclonal antibody is capable of specifically binding to CD 19. Within some embodiments, such inventive antibodies comprising one or more heavy and/or light chain CDR3 domain from a non- human antibody (a) are capable of competing for binding with; (b) retain the functional characteristics; (c) bind to the same epitope; and/or (d) have a similar binding affinity as the corresponding parental non-human antibody.
Within other aspects, the present disclosure provides monoclonal antibodies comprising one or more heavy and/or light chain CDR3 domain from a human antibody, such as, for example, a human antibody obtained from a non-human animal, wherein the human antibody is capable of specifically binding to CD 19. Within other aspects, the present disclosure provides monoclonal antibodies comprising one or more heavy and/or light chain CDR3 domain from a first human antibody, such as, for example, a human antibody obtained from a non-human animal, wherein the first human antibody is capable of specifically binding to CD 19 and wherein the CDR3 domain from the first human antibody replaces a CDR3 domain in a human antibody that is lacking binding specificity for CD 19 to generate a second human antibody that is capable of specifically binding to CD 19. Within some embodiments, such inventive antibodies comprising one or more heavy and/or light chain CDR3 domain from the first human antibody (a) are capable of competing for binding with; (b) retain the functional characteristics; (c) bind to the same epitope: and/or (d) have a similar binding affinity as the corresponding parental first human antibody.
Antibodies Having Particular Germline Sequences
In certain embodiments, an antibody of this disclosure comprises a heavy chain variable region from a particular germline heavy chain immunoglobulin gene and/or a light chain variable region from a particular germline light chain immunoglobulin gene.
For example, in a preferred embodiment, this disclosure provides an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a heavy chain variable region that is the product of or derived from a human VH 5-51 gene, wherein the antibody specifically binds CD 19. In another preferred embodiment, this disclosure provides an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a heavy chain variable region that is the product of or derived from a human VH 1-69 gene, wherein the antibody specifically binds CD 19. In yet another preferred embodiment, this disclosure provides an isolated monoclonal antibody, or an antigen- binding portion thereof, comprising a light chain variable region that is the product of or derived from a human VK Ll 8 gene, wherein the antibody specifically binds CD 19. In yet another preferred embodiment, this disclosure provides an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a light chain variable region that is the product of or derived from a human VK A27 gene, wherein the antibody specifically binds CD 19. In yet another preferred embodiment, this disclosure provides an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a light chain variable region that is the product of or derived from a human VK Ll 5 gene, wherein the antibody specifically binds CD 19. In yet another preferred embodiment, this disclosure provides an isolated monoclonal antibody, or antigen-binding portion thereof, wherein the antibody: (a) comprises a heavy chain variable region that is the product of or derived from a human Vn 5-51 or 1-69 gene (which genes encode the amino acid sequences set forth in SEQ ID NOs: 74 and 75, respectively);
(b) comprises a light chain variable region that is the product of or derived from a human VK L 18, Vκ A27 or Vκ Ll 5 gene (which genes encode the amino acid sequences set forth in SEQ ID NOs: 76, 77 and 78, respectively); and
(c) specifically binds to CD 19, preferably human CD 19.
Such antibodies also may possess one or more of the functional characteristics described in detail above, such as high affinity binding to human CD 19, internalization by CD 19-expressing cells, the ability to mediate ADCC against CD 19-expressing cells and/or the ability to inhibit tumor growth of CDl 9-expressing tumor cells in vivo when conjugated to a cytotoxin.
Examples of antibodies having VH and Vκ of VH 5-51 and VK L18, respectively. are 21D4, 21D4a, 27F3, 5G7, 13Fl and 46E8. An example of an antibody having VH and Vκ of VH 1-69 and VK A27, respectively, is 47G4. An example of an antibody having Vn and Vκ of Vn 1-69 and Vκ L15, respectively, is 3C10.
As used herein, a human antibody comprises heavy or light chain variable regions that is "the product of or '"derived from" a particular germline sequence if the variable regions of the antibody are obtained from a system that uses human germline immunoglobulin genes. Such systems include immunizing a transgenic mouse carrying human immunoglobulin genes with the antigen of interest or screening a human immunoglobulin gene library displayed on phage with the antigen of interest. A human antibody that is "the product of" or "derived from" a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequences of human germline immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence (i.e., greatest % identity) to the sequence of the human antibody. A human antibody that is "the product of or "derived from" a particular human germline immunoglobulin sequence may contain amino acid differences as compared to the gennline sequence, due to, for example, naturally-occurring somatic mutations or intentional introduction of site- directed mutation. However, a selected human antibody typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline immunoglobulin gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g., murine germline sequences). In certain cases, a human antibody may be at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene. Typically, a human antibody derived from a particular human germline sequence will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene. In certain cases, the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid sequence encoded by the germline immunoglobulin gene.
Homologous Antibodies
In yet another embodiment, an antibody of this disclosure comprises heavy and light chain variable regions comprising amino acid sequences that are homologous to the amino acid sequences of the preferred antibodies described herein, and wherein the antibodies retain the desired functional properties of the anti-CD 19 antibodies of this disclosure.
For example, this disclosure provides an isolated monoclonal antibody, or antigen binding portion thereof, comprising a heavy chain variable region and a light chain variable region, wherein:
(a) the heavy chain variable region comprises an amino acid sequence that is at least 80% homologous to an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6 and 7;
(b) the light chain variable region comprises an amino acid sequence that is at least 80% homologous to an amino acid sequence selected from the group consisting of
SEQ ID NOs: 8, 9, 10, 1 1, 12, 13, 14 and 15;
(c) the antibody binds to human CD 19 with a K0 of IxIO"7 M or less;
(d) binds to Raji and Daudi B-cell tumor cells.
Additionally or alternatively, the antibody may possess one or more of the following functional properties discussed above, such as high affinity binding to human CD 19, internalization by CD19-expressing cells, the ability to mediate ADCC against CD 19-expressing cells and/or the ability to inhibit tumor growth of CD 19-expressing tumor cells in vivo when conjugated to a cytotoxin.
In various embodiments, the antibody can be, for example, a human antibody, a humanized antibody or a chimeric antibody. In other embodiments, the VH and/or VL amino acid sequences may be 85%, 90%,
95%, 96%, 97%, 98% or 99% homologous to the sequences set forth above. An antibody having VH and VL regions having high (i.e., 80% or greater) homology to the Vn and VL regions of the sequences set forth above, can be obtained by mutagenesis (e.g., site- directed or PCR-mediated mutagenesis) of nucleic acid molecules encoding SEQ ID NOs: 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72 or 73, followed by testing of the encoded altered antibody for retained function (i.e., the functions set forth in (c) through (d) above) using the functional assays described herein.
As used herein, the percent homology between two amino acid sequences is equivalent to the percent identity between the two sequences. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % homology = # of identical positions/total # of positions x 100), taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences. The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm, as described in the non-limiting examples below.
The percent identity between two amino acid sequences can be determined using the algorithm of E. Meyers and W. Miller (Comput. Appl. Biosci., 4: 1 1-17 (1988)) which has been incorporated into the ALIGN program (version 2.0), using a PAM 120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. In addition, the percent identity between two amino acid sequences can be determined using the Needleman and Wunsch (J. MoI. Biol. 48:444-453 (1970)) algorithm which has been incorporated into the GAP program in the GCG software package (available at www.gcg.com), using either a Blossum 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6. Additionally or alternatively, the protein sequences of the present disclosure can further be used as a "query sequence" to perform a search against public databases to, for example, identify related sequences. Such searches can be performed using the XBLAST program (version 2.0) of Altschul, et al. (1990) J. MoI. Biol. 215:403-10. BLAST protein searches can be performed with the XBLAST program, score = 50, wordlength = 3 to obtain amino acid sequences homologous to the antibody molecules of tliis disclosure. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., (1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs (e.g., XBLAST and NBLAST) are useful. See www.ncbi.nlm.nih.gov.
Antibodies with Conservative Modifications In certain embodiments, an antibody of this disclosure comprises a heavy chain variable region comprising CDRl, CDR2 and CDR3 sequences and a light chain variable region comprising CDRl , CDR2 and CDR3 sequences, wherein one or more of these CDR sequences comprise specified amino acid sequences based on known anti-CD 19 antibodies, or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the anti-CD 19 antibodies of this disclosure. It is understood in the art that certain conservative sequence modification can be made which do not remove antigen binding. See, for example, Brummell et al. (1993) Biochem 32:1180-8 (describing mutational analysis in the CDR3 heavy chain domain of antibodies specific for Salmonella); de Wildt et al. (1997) Prot. Eng. 10:835-41 (describing mutation studies in anti-UAl antibodies); Komissarov et al. (1997) J. Biol. Chem. 272:26864-26870 (showing that mutations in the middle of HCDR3 led to either abolished or diminished affinity); Hall et al. (1992) J. Immunol. 149: 1605-12 (describing that a single amino acid change in the CDR3 region abolished binding activity); Kelley and O'Connell (1993) Biochem. 32:6862-35 (describing the contribution of Tyr residues in antigen binding); Adib-Conquy et al. (1998) Int. Immunol. 10:341-6 (describing the effect of hydrophobicity in binding) and Beers et al. (2000) Clin. Can. Res. 6:2835-43 (describing HCDR3 amino acid mutants). Accordingly, this disclosure provides an isolated monoclonal antibody, or antigen binding portion thereof, comprising a heavy chain variable region comprising CDRl, CDR2, and CDR3 sequences and a light chain variable region comprising CDRl, CDR2, and CDR3 sequences, wherein: (a) the heavy chain variable region CDR3 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36, and conservative modifications thereof;
(b) the light chain variable region CDR3 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequence of SEQ ID NOs: 51 , 52, 53, 54, 55, 56, 57 and 58, and conservative modifications thereof;
(c) the antibody binds to human CD 19 with a Kp of IxIO"7 M or less;
(d) binds to Raji and Daudi B-cell tumor cells.
Additionally or alternatively, the antibody may possess one or more of the following functional properties described above, such as high affinity binding to human CD 19, internalization by CD19-expressing cells, the ability to mediate ADCC against CD19-expressing cells and/or the ability to inhibit tumor growth of CD19-expressing tumor cells in vivo when conjugated to a cytotoxin.
In a preferred embodiment, the heavy chain variable region CDR2 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, and conservative modifications thereof; and the light chain variable region CDR2 sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50, and conservative modifications thereof. In another preferred embodiment, the heavy chain variable region CDRl sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22, and conservative modifications thereof; and the light chain variable region CDRl sequence comprises an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43, and conservative modifications thereof.
In various embodiments, the antibody can be, for example, human antibodies, humanized antibodies or chimeric antibodies.
As used herein, the term "conservative sequence modifications"' is intended to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence. Such conservative modifications include amino acid substitutions, additions and deletions. Modifications can be introduced into an antibody of this disclosure by standard techniques known in the art, such as site-directed mutagenesis and PCR-mediated mutagenesis. Conservative amino acid substitutions are ones in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine, tryptophan), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus, one or more amino acid residues within the CDR regions of an antibody of this disclosure can be replaced with other amino acid residues from the same side chain family and the altered antibody can be tested for retained function (/ e , the functions set forth in (c) through (d) above) using the functional assays described herein.
Antibodies that Bind to the Same Epitope as Anti-CD 19 Antibodies of this Disclosure In another embodiment, this disclosure provides antibodies that bind an epitope on human CD 19 recognized by any of the CD 19 monoclonal antibodies of this disclosure (i.e., antibodies that have the ability to cross-compete for binding to CD 19 with any of the monoclonal antibodies of this disclosure). In preferred embodiments, the reference antibody for cross-competition studies can be the monoclonal antibody 21D4 (having VH and VL sequences as shown in SEQ ID NOs: 1 and 8, respectively), or the monoclonal antibody 21D4a (having VH and VL sequences as shown in SEQ ID NOs: 1 and 9, respectively), or the monoclonal antibody 47G4 (having VH and VL sequences as shown in SEQ ID NOs: 2 and 10, respectively), or the monoclonal antibody 27F3 (having Vn and VL sequences as shown in SEQ ID NOs: 3 and 1 1, respectively), or the monoclonal antibody 3C10 (having VH and VL sequences as shown in SEQ ID NOs: 4 and 12, respectively), or the monoclonal antibody 5G7 (having VH and VL sequences as shown in SEQ ID NOs: 5 and 13, respectively), or the monoclonal antibody 13Fl (having VH and VL sequences as shown in SEQ ID NOs: 6 and 14, respectively), or the monoclonal antibody 46E8 (having Vn and Vi sequences as shown in SEQ ID NOs: 7 and 15, respectively. Such cross-competing antibodies can be identified based on their ability to cross-compete with 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 in standard CD 19 binding assays. Standard ELISA assays can be used in which a recombinant human CD 19 protein is immobilized on the plate, one of the antibodies is fluorescently labeled and the ability of non-labeled antibodies to compete off the binding of the labeled antibody is evaluated. Additionally or alternatively, BIAcore analysis can be used to assess the ability of the antibodies to cross-compete. For example, as described further in Example 3, epitope binning experiments using BIAcore demonstrated that the 3C10, 6A4 and 7Bl antibodies recognize and bind to distinct epitopes on CD 19. The ability of a test antibody to inhibit the binding of, for example, 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8, to human CD 19 demonstrates that the test antibody can compete with 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 for binding to human CD19 and thus binds to the same epitope on human CD 19 as 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8. In a preferred embodiment, the antibody that binds to the same epitope on human CD 19 as 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 is a human monoclonal antibody. Such human monoclonal antibodies can be prepared and isolated as described in the Examples.
Engineered and Modified Antibodies
An antibody of the invention can further be prepared using an antibody having one or more known CDl 9 antibody VH and/or V1 sequences can be used as starting material to engineer a modified antibody, which modified antibody may have altered properties as compared to the starting antibody. An antibody can be engineered by modifying one or more amino acids within one or both variable regions (i.e., VH and/or VL), for example, within one or more CDR regions and/or within one or more framework regions. Additionally or alternatively, an antibody can be engineered by modifying residues within the constant region(s), for example, to alter the effector function(s) of the antibody.
In certain embodiments, CDR grafting can be used to engineer variable regions of antibodies. Antibodies interact with target antigens predominantly through amino acid residues that are located in the six heavy and light chain complementarity determining regions (CDRs). For this reason, the amino acid sequences within CDRs are more diverse between individual antibodies than sequences outside of CDRs. Because CDR sequences are responsible for most antibody-antigen interactions, it is possible to express recombinant antibodies that mimic the properties of specific naturally occurring antibodies by constructing expression vectors that include CDR sequences from the specific naturally occurring antibody grafted onto framework sequences from a different antibody with different properties (see, e.g., Riechmann, L. et al. (1998) Nature 332:323- 327; Jones, P. et al. (1986) Nature 321:522-525; Queen, C. et al. (1989) Proe. Natl. Acad. See. U.S.A. 86: 10029-10033; U.S. Patent No. 5,225,539 to Winter, and U.S. Patent Nos. 5,530,101; 5,585,089; 5,693,762 and 6,180,370 to Queen et al.) Accordingly, another embodiment of this disclosure pertains to an isolated monoclonal antibody, or antigen binding portion thereof, comprising a heavy chain variable region comprising CDRl, CDR2, and CDR3 sequences comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22, SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, and SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36, respectively, and a light chain variable region comprising CDRl, CDR2, and CDR3 sequences comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43, SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50, and SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58, respectively. Thus, such antibodies contain the VH and VL CDR sequences of monoclonal antibodies 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 yet may contain different framework sequences from these antibodies.
Such framework sequences can be obtained from public DNA databases or published references that include germline antibody gene sequences. For example, germline DNA sequences for human heavy and light chain variable region genes can be found in the "VBase" human germline sequence database (available on the Internet at www.mrc-cpe.cam.ac.uk/vbase), as well as in Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242; Tomlinson, I. M., et al. (1992) "The Repertoire of Human Germline VH Sequences Reveals about Fifty Groups of VH Segments with Different Hypervariable Loops" J. MoL Biol. 227:776-798; and Cox, J. P. L. et al. (1994) "A Directory of Human Germ-line VH Segments Reveals a Strong Bias in their Usage" Eur. J. Immunol . 24:827-836; the contents of each of which are expressly incorporated herein by reference. As another example, the germline DNA sequences for human heavy and light chain variable region genes can be found in the Genbank database. For example, the following heavy chain germline sequences found in the HCo7 HuMAb mouse are available in the accompanying Genbank accession numbers: 1-69
(NG_0010109, NTJ)24637 and BC070333), 3-33 (NG_0010109 and NTJ)24637) and 3- 7 (NGJ3O1O1O9 and NT_024637). As another example, the following heavy chain germline sequences found in the HCo 12 HuMAb mouse are available in the accompanying Genbank accession numbers: 1-69 (NGJ)OlO 109, NT_024637 and BC070333), 5-51 (NGJ)OlO 109 and NTJ)24637), 4-34 (NGJ)010109 and NT_024637), 3-30.3 (CAJ556644) and 3-23 (A.T406678). Yet another source of human heavy and light chain germline sequences is the database of human immunoglobulin genes available from IMGT (http://imgt.cines.fr).
Antibody protein sequences are compared against a compiled protein sequence database using one of the sequence similarity searching methods called the Gapped BLAST (Altschul et al. (1997) Nucleic Acids Research 25:3389-3402), which is well known to those skilled in the art. BLAST is a heuristic algorithm in that a statistically significant alignment between the antibody sequence and the database sequence is likely to contain high-scoring segment pairs (HSP) of aligned words. Segment pairs whose scores cannot be improved by extension or trimming is called a hit. Briefly, the nucleotide sequences of VBASE origin {http://vbase.mrc-cpe.cam.ac.uk/vbase 1 /Iist2.php) are translated and the region between and including FRl through FR3 framework region is retained. The database sequences have an average length of 98 residues. Duplicate sequences which are exact matches over the entire length of the protein are removed. A BLAST search for proteins using the program blastp with default, standard parameters except the low complexity filter, which is turned off, and the substitution matrix of BLOSUM62, filters for top 5 hits yielding sequence matches. The nucleotide sequences are translated in all six frames and the frame with no stop codons in the matching segment of the database sequence is considered the potential hit. This is in turn confirmed using the BLAST program tblastx, which translates the antibody sequence in all six frames and compares those translations to the VBASE nucleotide sequences dynamically translated in all six frames. Other human germline sequence databases, such as that available from IMGT (http://imgt.cines.fr), can be searched similarly to VBASE as described above.
The identities are exact amino acid matches between the antibody sequence and the protein database over the entire length of the sequence. The positives (identities + substitution match) are not identical but amino acid substitutions guided by the BLOSUM62 substitution matrix. If the antibody sequence matches two of the database sequences with same identity, the hit with most positives would be decided to be the matching sequence hit. Preferred framework sequences for use in the antibodies of this disclosure are those that are structurally similar to the framework sequences used by selected antibodies of this disclosure, e.g., similar to the Vn 5-51 framework sequences (SEQ ID NO: 74) and/or the VH 1-69 framework sequences (SEQ ID NO: 75) and/or the VK L 18 framework sequences (SEQ ID NO: 76) and/or the Vκ A27 framework sequence (SEQ ID NO: 77) and/or the Vκ Ll 5 framework sequence (SEQ ID NO: 78) used by preferred monoclonal antibodies of this disclosure. The VH CDRl, CDR2, and CDR3 sequences, and the VK CDRl, CDR2, and CDR3 sequences, can be grafted onto framework regions that have the identical sequence as that found in the germline immunoglobulin gene from which the framework sequence derive, or the CDR sequences can be grafted onto framework regions that contain one or more mutations as compared to the germline sequences. For example, it has been found that in certain instances it is beneficial to mutate residues within the framework regions to maintain or enhance the antigen binding ability of the antibody (see e.g., U.S. Patent Nos. 5,530,101; 5,585,089; 5,693,762 and 6,180,370 to Queen et ai). Another type of variable region modification is to mutate amino acid residues within the VH and/or VK CDRl, CDR2 and/or CDR3 regions to thereby improve one or more binding properties {e.g., affinity) of the antibody of interest. Site-directed mutagenesis or PCR-mediated mutagenesis can be performed to introduce the mutation(s) and the effect on antibody binding, or other functional property of interest, can be evaluated in in vitro or in vivo assays as described herein and provided in the Examples. Preferably conservative modifications (as discussed above) are introduced. The mutations may be amino acid substitutions, additions or deletions, but are preferably substitutions. Moreover, typically no more than one, two, three, four or five residues within a CDR region are altered.
Accordingly, in another embodiment, the instant disclosure provides isolated anti- CD 19 monoclonal antibodies, or antigen binding portions thereof, comprising a heavy chain variable region comprising: (a) a Vu CDRl region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 16, 17, 18, 19, 20, 21 and 22; (b) a Vn CDR2 region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29; (c) a VH CDR3 region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 30, 31, 32, 33, 34, 35 and 36; (d) a VK CDRl region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43; (e) a VK CDR2 region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50; and (f) a Vκ CDR3 region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 51, 52, 53, 54,
55, 56, 57 and 58, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 51, 52, 53, 54, 55,
56, 57 and 58.
Engineered antibodies of this disclosure include those in which modifications have been made to framework residues within VH and/or V«, e.g. to improve the properties of the antibody. Typically such framework modifications are made to decrease the immunogenicity of the antibody. For example, one approach is to "backmutate'' one or more framework residues to the corresponding germline sequence. More specifically, an antibody that has undergone somatic mutation may contain framework residues that differ from the germline sequence from which the antibody is derived. Such residues can be identified by comparing the antibody framework sequences to the germline sequences from which the antibody is derived.
For example, Table 1 below shows a number of amino acid changes in the framework regions of the anti-PD-1 antibodies 17D8, 2D3, 4Hl, 5C4, 4Al 1, 7D3 and 5F4 that differ from the heavy chain parent germline sequence. To return one or more of the amino acid residues in the framework region sequences to their germline configuration, the somatic mutations can be "backmutated" to the germline sequence by, for example, site-directed mutagenesis or PCR-mediated mutagenesis.
Table 1. Modifications to antibodies 17D8, 2D3, 4Hl, . 5C4, 4Al l, 7D3 and 5F4 from the heavy chain germline : configuration.
Antι-CD19 Ab Amino acid position Amino acid of Original amino acid of germline antibody configuration
21D4 30 S T
77 R S
21D4a 30 S T
77 R S
47G4 24 D A
3C10 77 N S
88 A S
5G7 19 N K
77 N S
13Fl 19 Q K
28 T S
85 G S
46E8 19 Q K
28 T S
85 G S Another type of framework modification involves mutating one or more residues within the framework region, or even within one or more CDR regions, to remove T cell epitopes to thereby reduce the potential immunogenicity of the antibody. This approach is also referred to as "deimmunization" and is described in further detail in U.S. Patent Publication No. 20030153043 by Can- et al. In addition or alternative to modifications made within the framework or CDR regions, antibodies of this disclosure may be engineered to include modifications within the Fc region, typically to alter one or more functional properties of the antibody, such as serum half-life, complement fixation, Fc receptor binding, and/or antigen-dependent cellular cytotoxicity. Furthermore, an antibody of this disclosure may be chemically modified (e.g., one or more chemical moieties can be attached to the antibody) or be modified to alter its glycosylation, again to alter one or more functional properties of the antibody. Each of these embodiments is described in further detail below. The numbering of residues in the Fc region is that of the EU index of Kabat.
In one embodiment, the hinge region of CHl is modified such that the number of cysteine residues in the hinge region is altered, e.g., increased or decreased. This approach is described further in U.S. Patent No. 5,677,425 by Bodmer et al. The number of cysteine residues in the hinge region of CHl is altered to, for example, facilitate assembly of the light and heavy chains or to increase or decrease the stability of the antibody.
In another embodiment, the Fc hinge region of an antibody is mutated to decrease the biological half life of the antibody. More specifically, one or more amino acid mutations are introduced into the CH2-CH3 domain interface region of the Fc -hinge fragment such that the antibody has impaired Staphylococcyl protein A (SpA) binding relative to native Fc-hinge domain SpA binding. This approach is described in further detail in U.S. Patent No. 6, 165,745 by Ward et al.
In another embodiment, the antibody is modified to increase its biological half life. Various approaches are possible. For example, one or more of the following mutations can be introduced: T252L, T254S, T256F, as described in U.S. Patent No.
6,277,375 to Ward. Alternatively, to increase the biological half life, the antibody can be altered within the CHl or Ct. region to contain a salvage receptor binding epitope taken from two loops of a CH2 domain of an Fc region of an IgG, as described in U.S. Patent Nos. 5,869,046 and 6, 121,022 by Presta et al. In yet other embodiments, the Fc region is altered by replacing at least one amino acid residue with a different amino acid residue to alter the effector function(s) of the antibody. For example, one or more amino acids selected from amino acid residues 234, 235, 236, 237, 297, 318, 320 and 322 can be replaced with a different amino acid residue such that the antibody has an altered affinity for an effector ligand but retains the antigen- binding ability of the parent antibody. The effector ligand to which affinity is altered can be, for example, an Fc receptor or the Cl component of complement. This approach is described in further detail in U.S. Patent Nos. 5,624,821 and 5,648,260, both by Winter et al.
In another example, one or more amino acids selected from amino acid residues 329, 331 and 322 can be replaced with a different amino acid residue such that the antibody has altered CIq binding and/or reduced or abolished complement dependent cytotoxicity (CDC). This approach is described in further detail in U.S. Patent Nos. 6,194,551 by Idusogie et al.
In another example, one or more amino acid residues within amino acid positions 2316, 17, 18, 19, 20, 21 and 2239 are altered to thereby alter the ability of the antibody to fix complement. This approach is described further in PCT Publication WO 94/29351 by Bodmer et al.
In yet another example, the Fc region is modified to increase the ability of the antibody to mediate antibody dependent cellular cytotoxicity (ADCC) and/or to increase the affinity of the antibody for an Fcγ receptor by modifying one or more amino acids at the following positions: 238, 239. 248, 249, 252, 254, 255, 256, 258, 265, 267, 268, 269, 270, 272, 276, 278, 280, 283, 285, 286, 289, 290, 292, 293, 294, 295, 296, 298, 301, 303, 305, 307, 309, 312, 315, 320, 322, 324, 326, 327, 329, 330. 331, 333, 334, 335, 337, 338, 340, 360, 373. 376, 378, 382, 388, 389, 398, 414, 416, 419, 430, 434, 435, 437, 438 or 439. This approach is described further in PCT Publication WO 00/42072 by Presta. Moreover, the binding sites on human IgGl for FcγRl , FcγRII, FcγRIII and FcRn have been mapped and variants with improved binding have been described (see Shields, R. L. et al. (2001) J. Biol. Chem. 276:6591-6604). Specific mutations at positions 256, 290, 298, 333, 334 and 339 were shown to improve binding to FcγRIII. Additionally, the following combination mutants were shown to improve FcγRIII binding: T256A/S298A, S298A/E333A, S298A/K224A and S298A/E333A/K334A.
In still another embodiment, the C-terminal end of an antibody of the present invention is modified by the introduction of a cysteine residue as is described in U.S. Provisional Application Serial No. 60/957,271, which is hereby incorporated by reference in its entirety. Such modifications include, but are not limited to, the replacement of an existing amino acid residue at or near the C-terminus of a full-length heavy chain sequence, as well as the introduction of a cysteine-containing extension to the c-terminus of a full-length heavy chain sequence. In preferred embodiments, the cysteine-containing extension comprises the sequence alanine-alanine-cysteine (from N-terminal to C- terminal).
In preferred embodiments the presence of such C-teπninal cysteine modifications provide a location for conjugation of a partner molecule, such as a therapeutic agent or a marker molecule. In particular, the presence of a reactive thiol group, due to the C- terminal cysteine modification, can be used to conjugate a partner molecule employing the disulfide linkers described in detail below. Conjugation of the antibody to a partner molecule in this manner allows for increased control over the specific site of attachment. Furthermore, by introducing the site of attachment at or near the C-terminus, conjugation can be optimized such that it reduces or eliminates interference with the antibody's functional properties, and allows for simplified analysis and quality control of conjugate preparations.
In still another embodiment, the glycosylation of an antibody is modified. For example, an aglycoslated antibody can be made (i.e., the antibody lacks glycosylation). Glycosylation can be altered to, for example, increase the affinity of the antibody for antigen. Such carbohydrate modifications can be accomplished by, for example, altering one or more sites of glycosylation within the antibody sequence. For example, one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to thereby eliminate glycosylation at that site. Such aglycosylation may increase the affinity of the antibody for antigen. Such an approach is described in further detail in U.S. Patent Nos. 5.714,350 and 6,350,861 to Co et al. Additional approaches for altering glycosylation are described in further detail in U.S. Patent 7,214,775 to Hanai et al, U.S. Patent No. 6,737,056 to Presta, U.S. Pub No. 20070020260 to Presta, PCT Publication No. WO/2007/084926 to Dickey et al. , PCT Publication No. WO/2006/089294 to Zhu et al., and PCT Publication No. WO/2007/055916 to Ravetch et al., each of which is hereby incorporated by reference in its entirety.
Additionally or alternatively, an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GlcNac structures. Such altered glycosylation patterns have been demonstrated to increase the ADCC ability of antibodies. Such carbohydrate modifications can be accomplished by, for example, expressing the antibody in a host cell with altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which to express recombinant antibodies of this disclosure to thereby produce an antibody with altered glycosylation. For example, the cell lines Ms704, Ms705, and Ms709 lack the fucosyltransferase gene, FUT8 (alpha ( 1 ,6) fucosyltransferase), such that antibodies expressed in the Ms704, Ms705, and Ms709 cell lines lack fucose on their carbohydrates. The Ms704, Ms705, and Ms709 FUT8~;~ cell lines were created by the targeted disruption of the FUT8 gene in CHO/DG44 cells using two replacement vectors (see U.S. Patent Publication No. 20040110704 by Yamane et al. and Yamane-Ohnuki et al. (2004) Biotechnol Bioeng 87:614-22). As another example, EP 1 , 176, 195 by Hanai et al. describes a cell line with a functionally disrupted FUT8 gene, which encodes a fucυsv i transferase, such that antibodies expressed in such a cell line exhibit hypo fixcosylat ion by reducing or eliminating the alpha 1,6 bond-related enzyme. Hanai et al. also describe cell lines which have a low enzyme activity for adding fucose to the N-acetylglucosamine that binds to the Fc region of the antibody or does not have the enzyme activity, for example the rat myeloma cell line YB2/0 (ATCC CRL 1662). PCT Publication WO 03/035835 by Presta describes a variant CHO cell line, Lee 13 cells, with reduced ability to attach fucose to Asn(297)-linked carbohydrates, also resulting in hypofucosylation of antibodies expressed in that host cell (see also Shields, R.L. et al. (2002) J. Biol. Client. 277:26733-26740). PCT Publication WO 99/54342 by Umana et al. describes cell lines engineered to express glycoprotein-modifying glycosyl transferases {e.g., beta( l,4)-N- acetylglucosaminyltransferase III (GnTIII)) such that antibodies expressed in the engineered cell lines exhibit increased bisecting GlcNac structures which results in increased ADCC activity of the antibodies (see also Umana et al. (1999) Nat. Biotech. j_7: 176-180). Alternatively, the fucose residues of the antibody may be cleaved off using a fucosidase enzyme. For example, the fucosidase alpha-L-fucosidase removes fucosyl residues from antibodies (Tarentino, A. L. et al. (1975) Biochem. 14:5516-23). Additionally or alternatively, an antibody can be made that has an altered type of glycosylation, wherein that alteration relates to the level of sialyation of the antibody. Such alterations are described in PCT Publication No. WO/2007/084926 to Dickey et al , and PCT Publication No. WO/2007/055916 to Ravetch et al., both of which are incorporated by reference in their entirety. For example, one may employ an enzymatic reaction with sialidase, such as, for example, Arthrobacter ureafacens sialidase. The conditions of such a reaction are generally described in the U.S. Patent No. 5,831 ,077, which is hereby incorporated by reference in its entirety. Other non-limiting examples of suitable enzymes are neuraminidase and N-GIycosidase F, as described in Schloemer et al . , ]. Virology, 15(4), 882-893 (1975) and in Leibiger et al . , Biochem J., 338, 529-538 (1999), respectively. Desialylated antibodies may be further purified by using affinity chromatography. Alternatively, one may employ methods to increase the level of sialyation, such as by employing sialytransferase enzymes. Conditions of such a reaction are generally described in Basset et al., Scandinavian Journal of Immunology, 51(3), 307-31 1 (2000).
Another modification of the antibodies herein that is contemplated by this disclosure is pegylation. An antibody can be pegylated to, for example, increase the biological (e.g., serum) half life of the antibody. To pegylate an antibody, the antibody, or fragment thereof, typically is reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions in which one or more PEG groups become attached to the antibody or antibody fragment. Preferably, the pegylation is earned out via an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous reactive water-soluble polymer). As used herein, the term "polyethylene glycol" is intended to encompass any of the forms of PEG that have been used to derivatize other proteins, such as mono (Cl-Cl 0) alkoxy- or aryloxy- polyethylene glycol or polyethylene glycol-maleirnide. In certain embodiments, the antibody to be pegylated is an aglycosylated antibody. Methods for pegylating proteins are known in the art and can be applied to the antibodies of this disclosure. See for example, EP 0 154 316 by Nishimura et al. and EP 0401 384 by Ishikawa et al.
Antibody Fragments and Antibody Mimetics The instant invention is not limited to traditional antibodies and may be practiced through the use of antibody fragments and antibody mimetics. As detailed below, a wide variety of antibody fragment and antibody mimetic technologies have now been developed and are widely known in the art. While a number of these technologies, such as domain antibodies, Nanobodies, and UniBodies make use of fragments of, or other modifications to, traditional antibody structures, there are also alternative technologies, such as Affibodies, DARPins, Anticalins, Avimers, and Versabodies that employ binding structures that, while they mimic traditional antibody binding, are generated from and function via distinct mechanisms.
Domain Antibodies (dAbs) are the smallest functional binding units of antibodies, corresponding to the variable regions of either the heavy (VH) or light (VL) chains of human antibodies. Domain Antibodies have a molecular weight of approximately 13 kDa. Domantis has developed a series of large and highly functional libraries of fully human VH and VL dAbs (more than ten billion different sequences in each library), and uses these libraries to select dAbs that are specific to therapeutic targets. In contrast to many conventional antibodies, Domain Antibodies are well expressed in bacterial, yeast, and mammalian cell systems. Further details of domain antibodies and methods of production thereof may be obtained by reference to U.S. Patent 6,291, 158; 6,582,915; 6,593,081 ; 6,172,197; 6,696,245; U.S. Serial No. 2004/01 10941; European patent application No. 1433846 and European Patents 0368684 & 0616640; WO05/035572, WO04/101790, WO04/081026, WO04/058821, WO04/003019 and WO03/002609, each of which is herein incorporated by reference in its entirety.
Nanobodies are antibody-derived therapeutic proteins that contain the unique structural and functional properties of naturally-occurring heavy-chain antibodies. These heavy-chain antibodies contain a single variable domain (VHH) and two constant domains (CH2 and CH3). Importantly, the cloned and isolated VHH domain is a perfectly stable polypeptide harboring the full antigen-binding capacity of the original heavy-chain antibody. Nanobodies have a high homology with the VH domains of human antibodies and can be further humanized without any loss of activity. Importantly, Nanobodies have a low immunogenic potential, which has been confirmed in primate studies with Nanobody lead compounds. Nanobodies combine the advantages of conventional antibodies with important features of small molecule drags. Like conventional antibodies, Nanobodies show high target specificity, high affinity for their target and low inherent toxicity. However, like small molecule drugs they can inhibit enzymes and readily access receptor clefts. Furthermore, Nanobodies are extremely stable, can be administered by means other than injection (see, e.g., WO 04/041867, which is herein incoiporated by reference in its entirety) and are easy to manufacture. Other advantages of Nanobodies include recognizing uncommon or hidden epitopes as a result of their small size, binding into cavities or active sites of protein targets with high affinity and selectivity due to their unique 3 -dimensional, drug format flexibility, tailoring of half-life and ease and speed of drug discovery. Nanobodies are encoded by single genes and are efficiently produced in almost all prokaryotic and eukaryotic hosts, e.g., E. coli (see, e.g., U.S. 6,765,087, which is herein incorporated by reference in its entirety), molds (for example Aspergillus or Trichoderma) and yeast (for example Saccharomyces, Kluyveromyces, Hansenula or Pichia) (see, e.g., U.S. 6,838,254, which is herein incoiporated by reference in its entirety). The production process is scalable and multi-kilogram quantities of
Nanobodies have been produced. Because Nanobodies exhibit a superior stability compared with conventional antibodies, they can be formulated as a long shelf-life, ready-to-use solution.
The Nanoclone method (see, e.g., WO 06/079372, which is herein incoiporated by reference in its entirety) is a proprietary method for generating Nanobodies against a desired target, based on automated high-throughout selection of B-cells and could be used in the context of the instant invention.
UniBodies are another antibody fragment technology, however this one is based upon the removal of the hinge region of IgG4 antibodies. The deletion of the hinge region results in a molecule that is essentially half the size of traditional IgG4 antibodies and has a univalent binding region rather than the bivalent binding region of IgG4 antibodies. It is also well known that IgG4 antibodies are inert and thus do not interact with the immune system, which may be advantageous for the treatment of diseases where an immune response is not desired, and this advantage is passed onto UniBodies. For example, UniBodies may function to inhibit or silence, but not kill, the cells to which they are bound. Additionally, UniBody binding to cancer cells do not stimulate them to proliferate. Furthermore, because UniBodies are about half the size of traditional IgG4 antibodies, they may show better distribution over larger solid tumors with potentially advantageous efficacy. UniBodies are cleared from the body at a similar rate to whole IgG4 antibodies and are able to bind with a similar affinity for their antigens as whole antibodies. Further details of UniBodies may be obtained by reference to patent application WO2007/059782, which is herein incorporated by reference in its entirety.
Affibody molecules represent a new class of affinity proteins based on a 58-amino acid residue protein domain, derived from one of the igG-binding domains of staphylococcal protein A. This three helix bundle domain has been used as a scaffold for the construction of combinatorial phagemid libraries, from which Affibody valiants that target the desired molecules can be selected using phage display technology (Nord K, Gunneriusson E, Ringdahl J, Stahl S, Uhlen M, Nygren PA, Binding proteins selected from combinatorial libraries of an α-helical bacterial receptor domain, Nat Biotechnol 1997; 15:772-7. Ronmark J, Gronlund H, Uhlen M, Nygren PA, Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A, Eur J Biochem 2002;269:2647-55). The simple, robust structure of Affibody molecules in combination with their low molecular weight (6 kDa), make them suitable for a wide variety of applications, for instance, as detection reagents (Ronmark J, Hansson M, Nguyen T, et al, Construction and characterization of affibody-Fc chimeras produced in Escherichia coli, J Immunol Methods 2002;261 : 199-21 1 ) and to inhibit receptor interactions (Sandstorai K, Xu Z, Forsberg G, Nygren PA, Inhibition of the CD28-CD80 co-stimulation signal by a CD28-binding Affibody ligand developed by combinatorial protein engineering, Protein Eng 2003;16:691-7). Further details of Affibodies and methods of production thereof may be obtained by reference to U.S. Patent No. 5,831,012 which is herein incorporated by reference in its entirety.
Labeled Affibodies may also be useful in imaging applications for determining abundance of Isoforms.
DARPins (Designed Ankyrin Repeat Proteins) are one example of an antibody mimetic DRP (Designed Repeat Protein) technology that has been developed to exploit the binding abilities of non-antibody polypeptides. Repeat proteins such as ankyrin or leucine-rich repeat proteins, are ubiquitous binding molecules, which occur, unlike antibodies, intra- and extracellularly. Their unique modular architecture features repeating structural units (repeats), which stack together to form elongated repeat domains displaying variable and modular target-binding surfaces. Based on this modularity, combinatorial libraries of polypeptides with highly diversified binding specificities can be generated. This strategy includes the consensus design of self- compatible repeats displaying variable surface residues and their random assembly into repeat domains.
DARPins can be produced in bacterial expression systems at very high yields and they belong to the most stable proteins known. Highly specific, high-affinity DARPins to a broad range of target proteins, including human receptors, cytokines, kinases, human proteases, viruses and membrane proteins, have been selected. DARPins having affinities in the single-digit nanomolar to picomolar range can be obtained.
DARPins have been used in a wide range of applications, including ELISA, sandwich ELISA, flow cytometric analysis (FACS), immunohistochemistry (IHC), chip applications, affinity purification or Western blotting. DARPins also proved to be highly active in the intracellular compartment for example as intracellular marker proteins fused to green fluorescent protein (GFP). DARPins were further used to inhibit viral entry with IC50 in the pM range. DARPins are not only ideal to block protein-protein interactions, but also to inhibit enzymes. Proteases, kinases and transporters have been successfully inhibited, most often an allosteric inhibition mode. Very fast and specific enrichments on the tumor and very favorable tumor to blood ratios make DARPins well suited for in vivo diagnostics or therapeutic approaches.
Additional information regarding DARPins and other DRP technologies can be found in U.S. Patent Application Publication No. 2004/0132028 and International Patent Application Publication No. WO 02/20565, both of which are hereby incorporated by reference in their entirety.
Anticalins are an additional antibody mimetic technology, however in this case the binding specificity is derived from lipocalins, a family of low molecular weight proteins that are naturally and abundantly expressed in human tissues and body fluids. Lipocalins have evolved to perform a range of functions in vivo associated with the physiological transport and storage of chemically sensitive or insoluble compounds. Lipocalins have a robust intrinsic structure comprising a highly conserved β-barrel which supports four loops at one terminus of the protein. These loops form the entrance to a binding pocket and conformational differences in this part of the molecule account for the variation in binding specificity between individual lipocalins. While the overall structure of hypervariable loops supported by a conserved β- sheet framework is reminiscent of immunoglobulins, lipocalins differ considerably from antibodies in terms of size, being composed of a single polypeptide chain of 160-180 amino acids which is marginally larger than a single immunoglobulin domain.
Lipocalins are cloned and their loops are subjected to engineering in order to create Anticalins. Libraries of structurally diverse Anticalins have been generated and Anticalin display allows the selection and screening of binding function, followed by the expression and production of soluble protein for further analysis in prokaryotic or eukaryotic systems. Studies have successfully demonstrated that Anticalins can be developed that are specific for virtually any human target protein can be isolated and binding affinities in the nanomolar or higher range can be obtained.
Anticalins can also be formatted as dual targeting proteins, so-called Duocalins. A Duocalin binds two separate therapeutic targets in one easily produced monomeric protein using standard manufacturing processes while retaining target specificity and affinity regardless of the structural orientation of its two binding domains. Modulation of multiple targets through a single molecule is particularly advantageous in diseases known to involve more than a single causative factor. Moreover, bi- or multivalent binding formats such as Duocalins have significant potential in targeting cell surface molecules in disease, mediating agonistic effects on signal transduction pathways or inducing enhanced internalization effects via binding and clustering of cell surface receptors. Furthermore, the high intrinsic stability of Duocalins is comparable to monomeric Anticalins, offering flexible formulation and delivery potential for Duocalins.
Additional information regarding Anticalins can be found in U.S. Patent No. 7,250,297 and International Patent Application Publication No. WO 99/16873, both of which are hereby incorporated by reference in their entirety. Another antibody mimetic technology useful in the context of the instant invention are Avimers. Avimers are evolved from a large family of human extracellular receptor domains by in vitro exon shuffling and phage display, generating multidomain proteins with binding and inhibitory properties. Linking multiple independent binding domains has been shown to create avidity and results in improved affinity and specificity compared with conventional single-epitope binding proteins. Other potential advantages include simple and efficient production of multitarget-specific molecules in Escherichia coli, improved thermostability and resistance to proteases. Avimers with sub-nanomolar affinities have been obtained against a variety of targets. Additional information regarding Avimers can be found in U.S. Patent
Application Publication Nos. 2006/0286603, 2006/0234299, 2006/02231 14, 2006/0177831, 2006/0008844, 2005/0221384, 2005/0164301, 2005/0089932, 2005/0053973, 2005/0048512, 2004/0175756, all of which are hereby incorporated by reference in their entirety. Versabodies are another antibody mimetic technology that could be used in the context of the instant invention. Versabodies are small proteins of 3-5 kDa with >15% cysteines, which form a high disulfide density scaffold, replacing the hydrophobic core that typical proteins have. The replacement of a large number of hydrophobic amino acids, comprising the hydrophobic core, with a small number of disulfides results in a protein that is smaller, more hydrophilic (less aggregation and non-specific binding), more resistant to proteases and heat, and has a lower density of T-cell epitopes, because the residues that contribute most to MHC presentation are hydrophobic. All four of these properties are well-known to affect immunogenicity, and together they are expected to cause a large decrease in immunogenicity. The inspiration for Versabodies comes from the natural injectable biopharmaceuticals produced by leeches, snakes, spiders, scorpions, snails, and anemones, which are known to exhibit unexpectedly low immunogenicity. Starting with selected natural protein families, by design and by screening the size, hydrophobicity, proteolytic antigen processing, and epitope density are minimized to levels far below the average for natural injectable proteins. Given the structure of Versabodies, these antibody mimetics offer a versatile format that includes multi-valency, multi-specificity, a diversity of half-life mechanisms, tissue targeting modules and the absence of the antibody Fc region. Furthermore, Versabodies are manufactured in E. coli at high yields, and because of their hydrophilicity and small size, Versabodies are highly soluble and can be formulated to high concentrations. Versabodies are exceptionally heat stable (they can be boiled) and offer extended shelf-life.
Additional information regarding Versabodies can be found in U.S. Patent Application Publication No. 2007/0191272 which is hereby incorporated by reference in its entirety.
The detailed description of antibody fragment and antibody mimetic technologies provided above is not intended to be a comprehensive list of all technologies that could be used in the context of the instant specification. For example, and also not by way of limitation, a variety of additional technologies including alternative polypeptide-based technologies, such as fusions of complimentary determining regions as outlined in Qui et al., Nature Biotechnology, 25(8) 921-929 (2007), which is hereby incorporated by reference in its entirety, as well as nucleic acid-based technologies, such as the RNA aptamer technologies described in U.S. Patent Nos. 5,789, 157, 5,864,026, 5,712,375, 5,763,566, 6,013,443, 6,376,474, 6,613,526, 6,1 14,120, 6,261,774, and 6,387,620, all of which are hereby incorporated by reference, could be used in the context of the instant invention.
Antibody Physical Properties
The antibodies of the present disclosure may be further characterized by the various physical properties of the anti-CD 19 antibodies. Various assays may be used to detect and/or differentiate different classes of antibodies based on these physical properties.
In some embodiments, antibodies of the present disclosure may contain one or more glycosylation sites in either the light or heavy chain variable region. The presence of one or more glycosylation sites in the variable region may result in increased immunogenicity of the antibody or an alteration of the pK of the antibody due to altered antigen binding (Marshall et al (1972) Annu Rev Biochem 41:673-702; Gala FA and Morrison SL (2004) J Immunol 172:5489-94; Wallick et al (1988) J Exp Med 168: 1099- 109; Spiro RG (2002) Glycobiology I2:43R-56R; Parekh et al (1985) Nature 316:452-7; Mimura et al. (2000) MoI Immunol 3J7:697-706). Glycosylation has been known to occur at motifs containing an N-X-S/T sequence. Variable region glycosylation may be tested using a Glycoblot assay, which cleaves the antibody to produce a Fab, and then tests for glycosylation using an assay that measures periodate oxidation and Schiff base formation. Alternatively, variable region glycosylation may be tested using Dionex light chromatography (Dionex-LC), which cleaves saccharides from a Fab into monosaccharides and analyzes the individual saccharide content. In some instances, it is preferred to have an anti-CD 19 antibody that does not contain variable region glycosylation. This can be achieved either by selecting antibodies that do not contain the glycosylation motif in the variable region or by mutating residues within the glycosylation motif using standard techniques well known in the art.
In a preferred embodiment, the antibodies of the present disclosure do not contain asparagine isomerism sites. A deamidation or isoaspartic acid effect may occur on N-G or D-G sequences, respectively. The deamidation or isoaspartic acid effect results in the creation of isoaspartic acid which decreases the stability of an antibody by creating a kinked structure off a side chain carboxy terminus rather than the main chain. The creation of isoaspartic acid can be measured using an iso-quant assay, which uses a reverse-phase HPLC to test for isoaspartic acid.
Each antibody will have a unique isoelectric point (pi), but generally antibodies will fall in the pH range of between 6 and 9.5. The pi for an IgGl antibody typically falls within the pH range of 7-9.5 and the pi for an IgG4 antibody typically falls within the pH range of 6-8. Antibodies may have a pi that is outside this range. Although the effects are generally unknown, there is speculation that antibodies with a pi outside the normal range may have some unfolding and instability under in vivo conditions. The isoelectric point may be tested using a capillary isoelectric focusing assay, which creates a pH gradient and may utilize laser focusing for increased accuracy (Janini et al (2002) Electrophoresis 23: 1605-1 1 ; Ma et al. (2001 ) Chromatographic! 53:S75-89; Hunt et al ( 1998) J Chromatogr A 800:355-67). In some instances, it is preferred to have an anti- CD 19 antibody that contains a pi value that falls in the normal range. This can be achieved either by selecting antibodies with a pi in the normal range, or by mutating charged surface residues using standard techniques well known in the art.
Each antibody will have a melting temperature that is indicative of thermal stability (Krishnamurthy R and Manning MC (2002) Curr Pharm Biotechnol 3:361-71). A higher thermal stability indicates greater overall antibody stability in vivo. The melting point of an antibody may be measured using techniques such as differential scanning calorimetry (Chen et al (2003) Pharm Res 20: 1952-60; Ghirlando et al (1999) Immunol Lett 68:47-52). TM I indicates the temperature of the initial unfolding of the antibody. TM2 indicates the temperature of complete unfolding of the antibody. Generally, it is preferred that the TM i of an antibody of the present disclosure is greater than 600C, preferably greater than 65°C, even more preferably greater than 7O0C. Alternatively, the thermal stability of an antibody may be measured using circular dichroism (Murray et al. (2002) J. Chromatogr Sci 40:343-9).
In a preferred embodiment, antibodies are selected that do not rapidly degrade. Fragmentation of an anti-CD 19 antibody may be measured using capillary electrophoresis (CE) and MALDI-MS, as is well understood in the art (Alexander AJ and Hughes DE (1995) Anal Chem 67:3626-32).
In another preferred embodiment, antibodies are selected that have minimal aggregation effects. Aggregation may lead to triggering of an unwanted immune response and/or altered or unfavorable pharmacokinetic properties. Generally, antibodies are acceptable with aggregation of 25% or less, preferably 20% or less, even more preferably 15% or less, even more preferably 10% or less and even more preferably 5% or less. Aggregation may be measured by several techniques well known in the art, including size-exclusion column (SEC) high performance liquid chromatography (HPLC), and light scattering to identify monomers, dinners, trimers or multimers.
Methods of Engineering Antibodies
As discussed above, the anti-CD 19 antibodies having VH and VK sequences disclosed herein can be used to create new anti-CD 19 antibodies by modifying the VH and/or VK sequences, or the constant region(s) attached thereto. Thus, in another aspect of this disclosure, the staictural features of an anti-CD 19 antibody of this disclosure, e.g. 21 D4, 21 D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8, are used to create structurally related anti-CD 19 antibodies that retain at least one functional property of the antibodies of this disclosure, such as binding to human CD 19. For example, one or more CDR regions of 21 D4, 21 D4a, 47G4, 27F3, 3ClO, 5G7, 13Fl or 46E8, or mutations thereof, can be combined recombinantly with known framework regions and/or other CDRs to create additional, recombinantly-engineered, anti-CD 19 antibodies of this disclosure, as discussed above. Other types of modifications include those described in the previous section. The starting material for the engineering method is one or more of the VH and'or VK sequences provided herein, or one or more CDR regions thereof. To create the engineered antibody, it is not necessary to actually prepare (i.e., express as a protein) an antibody having one or more of the VH and/or VK sequences provided herein, or one or more CDR regions thereof. Rather, the information contained in the sequence(s) is used as the starting material to create a "second generation" sequence(s) derived from the original sequence(s) and then the "second generation" sequence(s) is prepared and expressed as a protein. Accordingly, in another embodiment, this disclosure provides a method for preparing an anti-CD 19 antibody comprising:
(a) providing: (i) a heavy chain variable region antibody sequence comprising a CDRl sequence selected from the group consisting of SEQ ID NOs: 16, 17, 18, 19, 20,
21 and 22, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 23, 24, 25, 26, 27, 28 and 29, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 30, 31 , 32, 33, 34, 35 and 36; and/or (ii) a light chain variable region antibody sequence comprising a CDR I sequence selected from the group consisting of SEQ ID NOs: 37, 38, 39, 40, 41, 42 and 43, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47, 48, 49 and 50, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 51, 52, 53, 54, 55, 56, 57 and 58;
(b) altering at least one amino acid residue within the heavy chain variable region antibody sequence and/or the light chain variable region antibody sequence to create at least one altered antibody sequence; and
(c) expressing the altered antibody sequence as a protein. Standard molecular biology techniques can be used to prepare and express the altered antibody sequence. Preferably, the antibody encoded by the altered antibody sequence(s) is one that retains one, some or all of the functional properties of the anti-CD 19 antibodies described herein, which functional properties include, but are not limited to:
(a) binds to human CD 19 with a KD of IxIO"7 M or less; (b) binds to Raji and Daudi B-cell tumor cells.
(c) is internalized by CD 19-expressing cells;
(d) exhibits antibody dependent cellular cytotoxicity (ADCC) against CD 19 expressing cells; and
(e) inhibits growth of CD 19-expressing cells in vivo when conjugated to a cytotoxin.
The functional properties of the altered antibodies can be assessed using standard assays available in the art and/or described herein, such as those set forth in the Examples (e.g.. flow cytometry, binding assays).
In certain embodiments of the methods of engineering antibodies of this disclosure, mutations can be introduced randomly or selectively along all or part of an anti-CD 19 antibody coding sequence and the resulting modified anti-CD 19 antibodies can be screened for binding activity and/or other functional properties as described herein. Mutational methods have been described in the art. For example, PCT Publication WO 02/092780 by Short describes methods for creating and screening antibody mutations using saturation mutagenesis, synthetic ligation assembly, or a combination thereof. Alternatively. PCT Publication WO 03/074679 by Lazar et al. describes methods of using computational screening methods to optimize physiochemical properties of antibodies.
Nucleic Acid Molecules Encoding Antibodies of this Disclosure Another aspect of this disclosure pertains to nucleic acid molecules that encode the antibodies of this disclosure. The nucleic acids may be present in whole cells, in a cell lysate, or in a partially purified or substantially pure form. A nucleic acid is "isolated" or "rendered substantially pure" when purified away from other cellular components or other contaminants, e.g., other cellular nucleic acids or proteins, by standard techniques, including alkaline/SDS treatment, CsCl banding, column chromatography, agarose gel electrophoresis and others well known in the art. See, F. Ausubel, et ai, ed. (1987) Current Protocols in Molecular Biology, Greene Publishing and Wiley Interscience, New York. A nucleic acid of this disclosure can be, for example, DNA or RNA and may or may not contain intronic sequences. In a preferred embodiment, the nucleic acid is a cDNA molecule. Nucleic acids of this disclosure can be obtained using standard molecular biology techniques. For antibodies expressed by hybridomas (e.g., hybridomas prepared from transgenic mice carrying human immunoglobulin genes as described further below), cDNAs encoding the light and heavy chains of the antibody made by the hybridoma can be obtained by standard PCR amplification or cDNA cloning techniques. For antibodies obtained from an immunoglobulin gene library (e.g., using phage display techniques), one or more nucleic acids encoding the antibody can be recovered from the library.
Preferred nucleic acids molecules of this disclosure are those encoding the Vn and VL sequences of the 21 D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl or 46E8 monoclonal antibodies. DNA sequences encoding the Vn sequences of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 59, 60, 61, 62, 63, 64 and 65, respectively. DNA sequences encoding the VL sequences of 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 are shown in SEQ ID NOs: 66, 67, 68, 69, 70, 71, 72 and 73, respectively.
Once DNA fragments encoding Vn and VL segments are obtained, these DNA fragments can be further manipulated by standard recombinant DNA techniques, for example to convert the variable region genes to full-length antibody chain genes, to Fab fragment genes or to a scFv gene. In these manipulations, a VL- or Vn-encoding DNA fragment is operatively linked to another DNA fragment encoding another protein, such as an antibody constant region or a flexible linker. The term "operatively linked", as used in this context, is intended to mean that the two DNA fragments are joined such that the amino acid sequences encoded by the two DNA fragments remain in-frame.
The isolated DNA encoding the VH region can be converted to a full-length heavy chain gene by operatively linking the VH-encoding DNA to another DNA molecule encoding heavy chain constant regions (CHl, CH2 and CH3). The sequences of human heavy chain constant region genes are known in the art (see e.g., Kabat, E. A., el al.
(1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242) and DNA fragments encompassing these regions can be obtained by standard PCR amplification. The heavy chain constant region can be an IgGl, IgG2, IgG3, IgG4, IgA, IgE, IgM or IgD constant region, but most preferably is an IgG 1 or IgG4 constant region. For a Fab fragment heavy chain gene, the Vπ-encoding DNA can be operatively linked to another DNA molecule encoding only the heavy chain CH 1 constant region.
The isolated DNA encoding the VL region can be converted to a full-length light chain gene (as well as a Fab light chain gene) by operatively linking the VL-encoding DNA to another DNA molecule encoding the light chain constant region, CL. The sequences of human light chain constant region genes are known in the art (see e.g., Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242) and DNA fragments encompassing these regions can be obtained by standard PCR amplification. In preferred embodiments, the light chain constant region can be a kappa or lambda constant region.
To create a scFv gene, the VH- and Vt-encoding DNA fragments are operatively linked to another fragment encoding a flexible linker, e.g., encoding the amino acid sequence (GIy4 -Ser)3, such that the VH and V] sequences can be expressed as a contiguous single-chain protein, with the V1 and VH regions joined by the flexible linker (see e.g., Bird et al. (1988) Science 242:423-426; Huston et al. (1988) Proc. Natl. Acad. ScL USA 85:5879-5883; McCafferty et al., (1990) Nature 348:552-554).
Production of Monoclonal Antibodies of this Disclosure
Monoclonal antibodies (mAbs) of the present disclosure can be produced by a variety of techniques, including conventional monoclonal antibody methodology e.g., the standard somatic cell hybridization technique of Kohler and Milstein (1975) Nature 256:
495. Although somatic cell hybridization procedures are preferred, in principle, other techniques for producing monoclonal antibody can be employed e.g., viral or oncogenic transformation of B lymphocytes.
The preferred animal system for preparing hybridomas is the murine system. Hybridoma production in the mouse is a very well-established procedure. Immunization protocols and techniques for isolation of immunized splenocytes for fusion are known in
7? the art. Fusion partners (e.g., murine myeloma cells) and fusion procedures are also known.
Chimeric or humanized antibodies of the present disclosure can be prepared based on the sequence of a non-human monoclonal antibody prepared as described above. DNA encoding the heavy and light chain immunoglobulins can be obtained from the non- human hybridoma of interest and engineered to contain non-murine (e.g., human) immunoglobulin sequences using standard molecular biology techniques. For example, to create a chimeric antibody, murine variable regions can be linked to human constant regions using methods known in the art (see e.g., U.S. Patent No. 4,816,567 to Cabilly et a L). To create a humanized antibody, murine CDR regions can be inserted into a human framework using methods known in the art (see e.g., U.S. Patent No. 5,225,539 to Winter, and U.S. Patent Nos. 5,530,101 ; 5,585,089; 5,693,762 and 6, 180,370 to Queen et al.).
In a preferred embodiment, the antibodies of this disclosure are human monoclonal antibodies. Such human monoclonal antibodies directed against CD 19 can be generated using transgenic or transchromosomic mice carrying parts of the human immune system rather than the mouse system. These transgenic and transchromosomic mice include mice referred to herein as the HuMAb Mouse* and KM Mouse*', respectively, and are collectively referred to herein as '"human Ig mice." The HuMAb Mouse* (Medarex®, Inc.) contains human immunoglobulin gene miniloci that encode unrearranged human heavy (μ and γ) and K light chain immunoglobulin sequences, together with targeted mutations that inactivate the endogenous μ and K chain loci (see e.g., Lonberg, et al. (1994) Nature 368(6474): 856- 859). Accordingly, the mice exhibit reduced expression of mouse IgM or K, and in response to immunization, the introduced human heavy and light chain transgenes undergo class switching and somatic mutation to generate high affinity human IgGκ monoclonal antibodies (Lonberg, N. et al. (1994), supra; reviewed in Lonberg, N. (1994) Handbook of Experimental Pharmacology 113 :49- 101 ; Lonberg, N. and Huszar, D. (1995) Intern. Rev. Immunol. L3: 65-93, and Harding, F. and Lonberg, N. (1995) Ann. N. Y. Acad. Sci. 764:536-546). Preparation and use of the HuMAb Mouse*, and the genomic modifications carried by such mice, is further described in Taylor, L. et al. (1992) Nucleic Acids Research 20:6287-6295; Chen, J. et al. (1993) International Immunology 5: 647-656; Tuaillon et al. (1993) Proc. Natl. Acad. ScL USA 90:3720-3724; Choi et al. (1993) Nature Genetics 4: 1 17-123; Chen, J. et al. (1993) EMBO J. U: 821- 830; Tuaillon et al. (1994) J. Immunol. 152:2912-2920; Taylor, L. et al. (1994) International Immunology 6: 579-591; and Fishwild, D. et al. (1996) Nature Biotechnology M: 845-851, the contents of all of which are hereby specifically incoiporated by reference in their entirety. See further, U.S. Patent Nos. 5,545,806; 5,569,825; 5,625,126; 5,633,425; 5,789,650; 5,877,397; 5,661,016; 5,814,318; 5,874,299; and 5,770,429; all to Lonberg and Kay; U.S. Patent No. 5,545,807 to Surani et al.; PCT Publication Nos. WO 92/03918, WO 93/12227, WO 94/25585, WO 97/13852, WO 98/24884 and WO 99/45962, all to Lonberg and Kay; and PCT Publication No. WO 01/14424 to Korman et al. Transgenic mice carrying human lambda light chain genes also can be used, such as those described in PCT Publication No. WO 00/26373 by Bruggemann. For example, a mouse carrying a human lambda light chain transgene can be crossbred with a mouse carrying a human heavy chain transgene (e.g., HCo7), and optionally also carrying a human kappa light chain transgene (e.g., KCo5) to create a mouse carrying both human heavy and light chain transgenes (see e.g.. Example 1).
In another embodiment, human antibodies of this disclosure can be raised using a mouse that carries human immunoglobulin sequences on transgenes and transchomosomes, such as a mouse that carries a human heavy chain transgene and a human light chain transchromosome. This mouse is referred to herein as a '"KM mouse®", and is described in detail in PCT Publication WO 02/43478 to Ishida et al.
Still further, alternative transgenic animal systems expressing human immunoglobulin genes are available in the art and can be used to raise anti-CD 19 antibodies of this disclosure. For example, an alternative transgenic system referred to as the Xenomouse (Abgenix, Inc.) can be used; such mice are described in, for example, U.S. Patent Nos. 5,939,598; 6,075, 181; 6,114,598; 6,150,584 and 6, 162,963 to Kucherlapati et al.
Moreover, alternative transchromosomic animal systems expressing human immunoglobulin genes are available in the art and can be used to raise anti-CD 19 antibodies of this disclosure. For example, mice carrying both a human heavy chain transchromosome and a human light chain tranchromosome, referred to as "TC mice" can be used; such mice are described in Tomizuka et al. (2000) Proc. Natl. Acad. Sci. USA 92:122-121. Furthermore, cows carrying human heavy and light chain transchromosomes have been described in the art {e.g., Kuroiwa et al. (2002) Nature Biotechnology 20:889-894 and PCT application No. WO 2002/092812) and can be used to raise anti-CD 19 antibodies of this disclosure.
Human monoclonal antibodies of this disclosure can also be prepared using phage display methods for screening libraries of human immunoglobulin genes. Such phage display methods for isolating human antibodies are established in the art. See for example: U.S. Patent Nos. 5,223,409; 5,403,484; and 5,571,698 to Ladner et al; U.S. Patent Nos. 5,427,908 and 5,580,717 to Dower et al.; U.S. Patent Nos. 5,969,108 and 6,172,197 to McCafferty et al.; and U.S. Patent Nos. 5,885,793; 6,521,404; 6,544,731; 6,555,313; 6,582,9130, 6,582,915 and 6,593,081 to Griffiths et al.
Human monoclonal antibodies of this disclosure can also be prepared using SCID mice into which human immune cells have been reconstituted such that a human antibody response can be generated upon immunization. Such mice are described in, for example, U.S. Patent Nos. 5,476,996 and 5,698,767 to Wilson et al.
In another embodiment, human anti-CD 19 antibodies are prepared using a combination of human Ig mouse and phage display techniques, as described in U.S. Patent No. 6,794,132 by Buechler et al. More specifically, the method first involves raising an anti-CD 19 antibody response in a human Ig mouse (such as a HuMab mouse or KM mouse as described above) by immunizing the mouse with one or more CD 19 antigens, followed by isolating nucleic acids encoding human antibody chains from lymphatic cells of the mouse and introducing these nucleic acids into a display vector {e.g., phage) to provide a library of display packages. Thus, each library member comprises a nucleic acid encoding a human antibody chain and each antibody chain is displayed from the display package. The library then is screened with CD 19 protein to isolate library members that specifically bind to CD 19. Nucleic acid inserts of the selected library members then are isolated and sequenced by standard methods to determine the light and heavy chain variable sequences of the selected CD 19 binders. The variable regions can be converted to full-length antibody chains by standard recombinant DNA techniques, such as cloning of the variable regions into an expression vector that carries the human heavy and light chain constant regions such that the Vn region is operatively linked to the Cn region and the V1 region is operatively linked to the CL region.
Immunization of Human Ig Mice
When human Ig mice are used to raise human antibodies of this disclosure, such mice can be immunized with a purified or enriched preparation of CD 19 antigen and/or recombinant CD 19, or cells expressing a CD 19 protein, or a CD 19 fusion protein, as described by Lonberg, N. et al. (1994) Nature 368(6474): 856-859; Fishwild, D. et al. (1996) Nature Biotechnology W: 845-851 ; and PCT Publication WO 98/24884 and WO 01/14424. Preferably, the mice will be 6-16 weeks of age upon the first infusion. For example, a purified or recombinant preparation (5-50 μg) of CD 19 antigen can be used to immunize the human Ig mice intraperitoneally and/or subcutaneously. Most preferably, the immunogen used to raise the antibodies of this disclosure is a CD 19 fusion protein comprising the extracellular domain of a CD 19 protein, fused at its N-terminus to a non- CD^ polypeptide (e.g., a His tag) (described further in Example 1).
Detailed procedures to generate fully human monoclonal antibodies that bind to human CD 19 are described in Example I below. Cumulative experience with various antigens has shown that the transgenic mice respond when initially immunized intraperitoneally (IP) with antigen in complete Freund's adjuvant, followed by every other week IP immunizations (up to a total of 6) with antigen in incomplete Freund's adjuvant. However, adjuvants other than Freund's are also found to be effective (e.g., RIBI adjuvant). In addition, whole cells in the absence of adjuvant are found to be highly immunogenic. The immune response can be monitored over the course of the immunization protocol with plasma samples being obtained by retroorbital bleeds. The plasma can be screened by ELISA (as described below), and mice with sufficient titers of anti-CD 19 human immunoglobulin can be used for fusions. Mice can be boosted intravenously with antigen, for example 3 days before sacrifice and removal of the spleen. It is expected that 2-3 fusions for each immunization may need to be performed. Between 6 and 24 mice are typically immunized for each antigen. Usually both HCo7 and HCo 12 strains are used. In addition, both HCo7 and HCo 12 transgene can be bred together into a single mouse having two different human heavy chain transgenes (HCo7/HCol2). Alternatively or additionally, the KM Mouse1" strain can be used..
Generation of Hybridomas Producing Human Monoclonal Antibodies of the Invention
To generate hybridomas producing human monoclonal antibodies of this disclosure, splenocytes and/or lymph node cells from immunized mice can be isolated and fused to an appropriate immortalized cell line, such as a mouse myeloma cell line. The resulting hybridomas can be screened for the production of antigen-specific antibodies. For example, single cell suspensions of splenic lymphocytes from immunized mice can be fused to one-sixth the number of P3X63-Ag8.653 nonsecreting mouse myeloma cells (ATCC, CRL 1580) with 50% PEG. Alternatively, the single cell suspension of splenic lymphocytes from immunized mice can be fused using an electric field based electrofusion method, using a CytoPulse large chamber cell fusion electroporator (CytoPulse Sciences, Inc., Glen Burnie Maryland). Cells are plated at approximately 2 x 10 in flat bottom microti ter plate, followed by a two week incubation in selective medium containing 20% fetal Clone Serum, 18% "653" conditioned media, 5% origen (IGEN), 4 mM L-glutamine, 1 mM sodium pyruvate, 5mM HEPES, 0.055 mM 2-mercaptoethanol, 50 units/ml penicillin, 50 mg/ml streptomycin, 50 mg/ml gentamycin and IX HAT (Sigma; the HAT is added 24 hours after the fusion). After approximately two weeks, cells can be cultured in medium in which the HAT is replaced with HT. Individual wells can then be screened by ELISA for human monoclonal IgM and IgG antibodies. Once extensive hybridoma growth occurs, medium can be observed usually after 10-14 days. The antibody secreting hybridomas can be replated, screened again, and if still positive for human IgG, the monoclonal antibodies can be subcloned at least twice by limiting dilution. The stable subclones can then be cultured in vitro to generate small amounts of antibody in tissue culture medium for characterization.
To purify human monoclonal antibodies, selected hybridomas can be grown in two-liter spinner-flasks for monoclonal antibody purification. Supernatants can be filtered and concentrated before affinity chromatography with protein A-sepharose (Pharmacia, Piscataway, NJ.). Eluted IgG can be checked by gel electrophoresis and high performance liquid chromatography to ensure purity. The buffer solution can be exchanged into PBS, and the concentration can be determined by OD280 using 1.43 extinction coefficient. The monoclonal antibodies can be aliquoted and stored at -80° C.
Generation of Transfectomas Producing Monoclonal Antibodies of the Invention
Antibodies of this disclosure also can be produced in a host cell transfectoma using, for example, a combination of recombinant DNA techniques and gene transfection methods as is well known in the art (e.g., Morrison, S. (1985) Science 229: 1202).
For example, to express the antibodies, or antibody fragments thereof, DNAs encoding partial or full-length light and heavy chains, can be obtained by standard molecular biology techniques (e.g., PCR amplification or cDNA cloning using a hybridoma that expresses the antibody of interest) and the DNAs can be inserted into expression vectors such that the genes are operatively linked to transcriptional and translational control sequences. In this context, the term "operatively linked" is intended to mean that an antibody gene is ligated into a vector such that transcriptional and translational control sequences within the vector serve their intended function of regulating the transcription and translation of the antibody gene. The expression vector and expression control sequences are chosen to be compatible with the expression host cell used. The antibody light chain gene and the antibody heavy chain gene can be inserted into separate vector or, more typically, both genes are inserted into the same expression vector. The antibody genes are inserted into the expression vector by standard methods (e.g., ligation of complementary restriction sites on the antibody gene fragment and vector, or blunt end ligation if no restriction sites are present). The light and heavy chain variable regions of the antibodies described herein can be used to create full-length antibody genes of any antibody isotype by inserting them into expression vectors already encoding heavy chain constant and light chain constant regions of the desired isotype such that the VH segment is operatively linked to the CM segment(s) within the vector and the VK segment is operatively linked to the C1. segment within the vector. Additionally or alternatively, the recombinant expression vector can encode a signal peptide that facilitates secretion of the antibody chain from a host cell. The antibody chain gene can be cloned into the vector such that the signal peptide is linked in-frame to the amino terminus of the antibody chain gene. The signal peptide can be an immunoglobulin signal peptide or a heterologous signal peptide (i.e., a signal peptide from a non- immunoglobulin protein).
In addition to the antibody chain genes, the recombinant expression vectors of this disclosure carry regulatory sequences that control the expression of the antibody chain genes in a host cell. The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements (e.g., polyadenylation signals) that control the transcription or translation of the antibody chain genes. Such regulatory sequences are described, for example, in Goeddel (Gene Expression Technology. Methods in Enzymology 185, Academic Press, San Diego, CA (1990)). It will be appreciated by those skilled in the art that the design of the expression vector, including the selection of regulatory sequences, may depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. Preferred regulatory sequences for mammalian host cell expression include viral elements that direct high levels of protein expression in mammalian cells, such as promoters and/or enhancers derived from cytomegalovirus (CMV), Simian Virus 40 (SV40), adenovirus, (e.g., the adenovirus major late promoter (AdMLP)) and polyoma. Alternatively, nonviral regulatory sequences may be used, such as the ubiquitin promoter or β-globin promoter. Still further, regulatory elements composed of sequences from different sources, such as the SRa promoter system, "which contains sequences from the SV40 early promoter and the long terminal repeat of human T cell leukemia virus type 1 (Takebe, Y. et al. (1988) MoL Cell. Biol. 8:466-472).
In addition to the antibody chain genes and regulatory sequences, the recombinant expression vectors of this disclosure may carry additional sequences, such as sequences that regulate replication of the vector in host cells (e.g., origins of replication) and selectable marker genes. The selectable marker gene facilitates selection of host cells into which the vector has been introduced (see, e.g., U.S. Pat. Nos. 4,399,216, 4,634,665 and 5,179,017, all by Axel et al ). For example, typically the selectable marker gene confers resistance to drugs, such as G418, hygromycin or methotrexate, on a host cell into which the vector has been introduced. Preferred selectable marker genes include the dihydrofolate reductase (DHFR) gene (for use in dhfr- host cells with methotrexate selection/amplification) and the neo gene (for G418 selection). For expression of the light and heavy chains, the expression vector(s) encoding the heavy and light chains is transfected into a host cell by standard techniques. The various forms of the term "transfection" are intended to encompass a wide variety of techniques commonly used for the introduction of exogenous DNA into a prokaryotic or eukaryotic host cell, e.g., electroporation, calcium-phosphate precipitation, DEAE- dextran transfection and the like. Although it is theoretically possible to express the antibodies of this disclosure in either prokaryotic or eukaryotic host cells, expression of antibodies in eukaryotic cells, and most preferably mammalian host cells, is the most preferred because such eukaryotic cells, and in particular mammalian cells, are more likely than prokaryotic cells to assemble and secrete a properly folded and immunologically active antibody. Prokaryotic expression of antibody genes has been reported to be ineffective for production of high yields of active antibody (Boss, M. A. and Wood, C. R. (1985) Immunology Today 6: 12-13).
Preferred mammalian host cells for expressing the recombinant antibodies of this disclosure include Chinese Hamster Ovary (CHO cells) (including dhfr' CHO cells, described in Urlaub and Chasin, (1980) Proc. Natl. Acad. Sci. USA 77:4216-4220, used with a DHFR selectable marker, e.g., as described in R. J. Kaufman and P. A. Sharp (1982) J. MoL Biol. 159:601-621), NSO myeloma cells, COS cells and SP2 cells. In particular, for use with NSO myeloma cells, another preferred expression system is the GS gene expression system disclosed in WO 87/04462 (to Wilson), WO 89/01036 (to Bebbington) and EP 338,841 (to Bebbington). When recombinant expression vectors encoding antibody genes are introduced into mammalian host cells, the antibodies are produced by culturing the host cells for a period of time sufficient to allow for expression of the antibody in the host cells or, more preferably, secretion of the antibody into the culture medium in which the host cells are grown. Antibodies can be recovered from the culture medium using standard protein purification methods.
Characterization of Antibody Binding to Antigen
Antibodies of this disclosure can be tested for binding to human CD 19 by, for example, standard ELISA. Briefly, microtiter plates are coated with purified at 0.25μg/ml in PBS, and then blocked with 5% bovine serum albumin in PBS. Dilutions of antibody (e.g., dilutions of plasma from CD19-immunized mice) are added to each well and incubated for 1-2 hours at 370C. The plates are washed with PBS/Tween and then incubated with secondary reagent (e.g., for human antibodies, a goat-anti-human IgG Fc- specific polyclonal reagent) conjugated to alkaline phosphatase for 1 hour at 37°C. After washing, the plates are developed with pNPP substrate (1 mg/ml), and analyzed at OD of 405-650. Preferably, mice which develop the highest titers will be used for fusions.
An ELISA assay as described above can also be used to screen for hybridomas that show positive reactivity with CD 19 immunogen. Hybridomas that bind with high avidity and/or affinity to a CD 19 protein are subcloned and further characterized. One clone from each hybridoma, which retains the reactivity of the parent cells (by ELISA), can be chosen for making a 5-10 vial cell bank stored at -1400C, and for antibody purification.
To purify anti-CD 19 antibodies, selected hybridomas can be grown in two-liter spinner-flasks for monoclonal antibody purification. Supernatants can be filtered and concentrated before affinity chromatography with protein A-sepharose (Pharmacia, Piscataway, NJ). Eluted IgG can be checked by gel electrophoresis and high performance liquid chromatography to ensure purity. The buffer solution can be exchanged into PBS, and the concentration can be determined by OD280 using 1.43 extinction coefficient. The monoclonal antibodies can be aliquoted and stored at -800C. To determine if the selected anti-CD 19 monoclonal antibodies bind to unique epitopes, each antibody can be biotinylated using commercially available reagents
(Pierce, Rockford, IL). Competition studies using unlabeled monoclonal antibodies and biotinylated monoclonal antibodies can be performed using CD 19 coated-ELISA plates as described above. Biotinylated inAb binding can be detected with a strep-avidin- alkaline phosphatase probe. To determine the isotype of purified antibodies, isotype ELISAs can be performed using reagents specific for antibodies of a particular isotype. For example, to determine the isotype of a human monoclonal antibody, wells of microtiter plates can be coated with 1 μg/ml of anti-human immunoglobulin overnight at 4°C. After blocking with 1% BSA, the plates are reacted with 1 μg /ml or less of test monoclonal antibodies or purified isotype controls, at ambient temperature for one to two hours. The wells can then be reacted with either human IgGl or human IgM-specific alkaline phosphatase-conjugated probes. Plates are developed and analyzed as described above.
Anti-CD 19 human IgGs can be further tested for reactivity with CD 19 antigen by Western blotting. Briefly, CD 19 can be prepared and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. After electrophoresis, the separated antigens are transferred to nitrocellulose membranes, blocked with 10% fetal calf serum, and probed with the monoclonal antibodies to be tested. Human IgG binding can be detected using anti-human IgG alkaline phosphatase and developed with BCIP/NBT substrate tablets (Sigma Chem. Co., St. Louis, Mo.). The binding specificity of an antibody of this disclosure may also be determined by monitoring binding of the antibody to cells expressing a CD 19 protein, for example by flow cytometry. Cells or cell lines that naturally expresses CD 19 protein, such OVCAR3, NCI-H226, CFPAC-I or KB cells (described further in Example 3), may be used or a cell line, such as a CHO cell line, may be transfected with an expression vector encoding CD 19 such that CD 19 is expressed on the surface of the cells. The transfected protein may comprise a tag, such as a myc-tag or a his-tag, preferably at the N-terminus, for detection using an antibody to the tag. Binding of an antibody of this disclosure to a CD 19 protein may be determined by incubating the transfected cells with the antibody, and detecting bound antibody. Binding of an antibody to the tag on the transfected protein may be used as a positive control.
Bispecific Molecules
In another aspect, the present disclosure features bispecific molecules comprising an anti-CD 19 antibody, or a fragment thereof, of this disclosure. An antibody of this disclosure, or antigen-binding portions thereof, can be derivatized or linked to another functional molecule, e.g., another peptide or protein (e.g., another antibody or ligand for a receptor) to generate a bispecific molecule that binds to at least two different binding sites or target molecules. The antibody of this disclosure may in fact be derivatized or linked to more than one other functional molecule to generate multispecific molecules that bind to more than two different binding sites and/or target molecules; such multispecific molecules are also intended to be encompassed by the term "bispecific molecule" as used herein. To create a bispecific molecule of this disclosure, an antibody of this disclosure can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovalent association or otherwise) to one or more other binding molecules, such as another antibody, antibody fragment, peptide or binding mimetic, such that a bispecific molecule results. Accordingly, the present disclosure includes bispecific molecules comprising at least one first binding specificity for CD 19 and a second binding specificity for a second target epitope. In a particular embodiment of this disclosure, the second target epitope is an Fc receptor, e.g., human FcγRI (CD64) or a human Fcα receptor (CD89). Therefore, this disclosure includes bispecific molecules capable of binding both to FcγR or FcαR expressing effector cells (e.g., monocytes, macrophages or polymorphonuclear cells
(PMNs)), and to target cells expressing CD 19. These bispecific molecules target CD 19 expressing cells to effector cell and trigger Fc receptor-mediated effector cell activities, such as phagocytosis of an CD 19 expressing cells, antibody dependent cell-mediated cytotoxicity (ADCC), cytokine release, or generation of superoxide anion. In an embodiment of this disclosure in which the bispecific molecule is multispecific, the molecule can further include a third binding specificity, in addition to an anti-Fc binding specificity and an anti-CD 19 binding specificity. In one embodiment, the third binding specificity is an anti-enhancement factor (EF) portion, e.g.. a molecule which binds to a surface protein involved in cytotoxic activity and thereby increases the immune response against the target cell. The "anti-enhancement factor portion" can be an antibody, functional antibody fragment or a ligand that binds to a given molecule, e.g., an antigen or a receptor, and thereby results in an enhancement of the effect of the binding determinants for the Fc receptor or target cell antigen. The "anti-enhancement factor portion" can bind an Fc receptor or a target cell antigen. Alternatively, the anti- enhancement factor portion can bind to an entity that is different from the entity to which the first and second binding specificities bind. For example, the anti-enhancement factor portion can bind a cytotoxic T-cell (e.g., via CD2, CD3, CD8, CD28, CD4, CD40, ICAM-I or other immune cell that results in an increased immune response against the target cell). In one embodiment, the bispecific molecules of this disclosure comprise as a binding specificity at least one antibody, or an antibody fragment thereof, including, e.g., an Fab, Fab', F(ab')2, Fv, Fd, dAb or a single chain Fv. The antibody may also be a light chain or heavy chain dimer, or any minimal fragment thereof such as a Fv or a single chain construct as described in U.S. Patent No. 4,946,778 to Ladner et al., the contents of which is expressly incorporated by reference. In one embodiment, the binding specificity for an Fcγ receptor is provided by a monoclonal antibody, the binding of which is not blocked by human immunoglobulin G (IgG). As used herein, the term "IgG receptor" refers to any of the eight γ-chain genes located on chromosome 1. These genes encode a total of twelve transmembrane or soluble receptor isoforms which are grouped into three Fcγ receptor classes: FcγRI (CD64), FcγRII(CD32), and FcγRIII (CD 16). In one preferred embodiment, the Fcγ receptor a human high affinity FcγRI. The human FcγRI is a 72 kDa molecule, which shows high affinity for monomeric IgG (10b - 10ή M"1).
The production and characterization of certain preferred anti-Fcγ monoclonal antibodies are described in PCT Publication WO 88/00052 and in U.S. Patent No. 4,954,617 to Fanger et al., the teachings of which are fully incorporated by reference herein. These antibodies bind to an epitope of FcγRI, FcγRII or FcγRIII at a site which is distinct from the Fcγ binding site of the receptor and, thus, their binding is not blocked substantially by physiological levels of IgG. Specific anti-FcγRI antibodies useful in this disclosure are mAb 22, mAb 32, mAb 44, mAb 62 and mAb 197. The hybridoma producing mAb 32 is available from the American Type Culture Collection, ATCC Accession No. HB9469. In other embodiments, the anti-Fcγ receptor antibody is a humanized form of monoclonal antibody 22 (H22). The production and characterization of the H22 antibody is described in Graziano, R. F. et al. (1995) J. Immunol 155 (10): 4996-5002 and PCT Publication WO 94/10332 to Tempest et al.. The H22 antibody producing cell line was deposited at the American Type Culture Collection under the designation HA022CL1 and has the accession no. CRL 1 1 177.
In still other preferred embodiments, the binding specificity for an Fc receptor is provided by an antibody that binds to a human IgA receptor, e.g., an Fc-alpha receptor (FcαRI (CD89)), the binding of which is preferably not blocked by human immunoglobulin A (IgA). The term "IgA receptor" is intended to include the gene product of one α-gene (FcαRI) located on chromosome 19. This gene is known to encode several alternatively spliced transmembrane isoforms of 55 to 1 10 kDa. FcαRI (CD89) is constitutively expressed on monocytes/macrophages, eosinophilic and neutrophilic granulocytes, but not on non-effector cell populations. FcαRI has medium affinity (« 5 χ 107 M"1) for both IgAl and IgA2, which is increased upon exposure to cytokines such as G-CSF or GM-CSF (Morton, H.C. et al. ( 1996) Critical Reviews in Immunology J_6:423-440). Four FcαRI-specific monoclonal antibodies, identified as A3, A59, A62 and A77, which bind FcαRI outside the IgA ligand binding domain, have been described (Monteiro, R.C. et al. (1992) J. Immunol. 148: 1764).
FcαRI and FcγRI are preferred trigger receptors for use in the bispecific molecules of this disclosure because they are ( 1 ) expressed primarily on immune effector cells, e.g., monocytes, PMNs, macrophages and dendritic cells; (2) expressed at high levels (e.g., 5,000-100,000 per cell); (3) mediators of cytotoxic activities (e.g., ADCC, phagocytosis); and (4) mediate enhanced antigen presentation of antigens, including self- antigens, targeted to them. While human monoclonal antibodies are preferred, other antibodies which can be employed in the bispecific molecules of this disclosure are murine, chimeric and humanized monoclonal antibodies.
The bispecific molecules of the present disclosure can be prepared by conjugating the constituent binding specificities, e.g., the anti-FcR and anti-CD 19 binding specificities, using methods known in the art. For example, each binding specificity of the bispecific molecule can be generated separately and then conjugated to one another. When the binding specificities are proteins or peptides, a variety of coupling or cross- linking agents can be used for covalent conjugation. Examples of cross-linking agents include protein A, carbodiimide, N-succinimidyl-S-acetyl-thioacetate (SATA), 5,5'- dithiobis(2-nitrobenzoic acid) (DTNB), o-phenylenedimaleimide (oPDM), N- succinimidyl-3-(2-pyridyldithio)propionate (SPDP), and sulfosuccinimidyl 4-(N- maleimidomethyl) cyclohaxane-1-carboxylate (sulfo-SMCC) (see e.g., Karpovsky et al. (1984) J. Exp. Med. 160: 1686; Liu, MA et al (1985) Proc. Natl. Acad. ScL USA 82:8648). Other methods include those described in Paulus (1985) Behring Ins. Mitt No. 78, 1 18-132; Brennan et al. ( 1985) Science 229:81 -83, and Glennie et al. ( 1987) J. Immunol. 139: 2367-2375). Preferred conjugating agents are SATA and sulfo-SMCC, both available from Pierce Chemical Co. (Rockford, IL).
When the binding specificities are antibodies, they can be conjugated via sulfhydryl bonding of the C-terminus hinge regions of the two heavy chains. In a particularly preferred embodiment, the hinge region is modified to contain an odd number of sulfhydryl residues, preferably one, prior to conjugation.
Alternatively, both binding specificities can be encoded in the same vector and expressed and assembled in the same host cell. This method is particularly useful where the bispecific molecule is a mAb x mAb, mAb x Fab, Fab x F(ab')2 or ligand x Fab fusion protein. A bispecific molecule of this disclosure can be a single chain molecule comprising one single chain antibody and a binding determinant, or a single chain bispecific molecule comprising two binding determinants. Bispecific molecules may comprise at least two single chain molecules. Methods for preparing bispecific molecules are described for example in U.S. Patent Numbers 5,260,203; 5,455,030; 4,881,175; 5,132,405; 5,091,513; 5,476,786; 5,013,653; 5,258,498; and 5,482,858, all of which are expressly incorporated herein by reference.
Binding of the bispecific molecules to their specific targets can be confirmed by, for example, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), FACS analysis, bioassay (e.g., growth inhibition), or Western Blot assay. Each of these assays generally detects the presence of protein-antibody complexes of particular interest by employing a labeled reagent (e.g., an antibody) specific for the complex of interest. For example, the FcR-antibody complexes can be detected using e.g., an enzyme-linked antibody or antibody fragment which recognizes and specifically binds to the antibody- FcR complexes. Alternatively, the complexes can be detected using any of a variety of other immunoassays. For example, the antibody can be radioactively labeled and used in a radioimmunoassay (RIA) (see, for example, Weintraub, B., Principles of Radioimmunoassays, Seventh Training Course on Radioligand Assay Techniques, The Endocrine Society, March, 1986, which is incorporated by reference herein). The radioactive isotope can be detected by such means as the use of aycounter or a scintillation counter or by autoradiography. Linkers
The present invention provides for antibody-partner conjugates where the antibody is linked to the partner through a chemical linker. In some embodiments, the linker is a peptidyl linker, and is depicted herein as (L4)p — F — (L1 )m. Other linkers include hydrazine and disulfide linkers, and is depicted herein as (L4)p — H — (L1 )m or (L4)p — J— (L1 )m , respectively. In addition to the linkers as being attached to the partner, the present invention also provides cleavable linker arms that are appropriate for attachment to essentially any molecular species. The linker arm aspect of the invention is exemplified herein by reference to their attachment to a therapeutic moiety. It will, however, be readily apparent to those of skill in the art that the linkers can be attached to diverse species including, but not limited to, diagnostic agents, analytical agents, biomolecules, targeting agents, detectable labels and the like.
The use of peptidyl and other linkers in antibody-partner conjugates is described in U.S. Provisional Patent Applications Serial Nos. 60/295,196; 60/295,259; 60/295342; 60/304,908; 60/572,667; 60/661 , 174; 60/669,871 ; 60/720,499; 60/730,804; and 60/735,657 and U.S. Patent Applications Serial Nos. 10/160,972; 10/161,234; 11/134,685; 1 1/134,826; and 1 1/398,854 and U.S. Patent No. 6,989,452 and PCT Patent Application No. PCT/US2006/37793, all of which are incoiporated herein by reference. Additional linkers are described in U.S. Patent No. 6,214,345 (Bristol-Myers Squibb), U.S. Pat. Appl. 2003/0096743 and U.S. Pat. Appl. 2003/0130189 (both to
Seattle Genetics), de Groot et al., J. Med. Chetn. 42, 5277 (1999); de Groot et al. J. Org. Chem. 43, 3093 (2000); de Groot et al., J. Med. Chem. 66, 8815, (2001); WO 02/083180 (Syntarga); Carl et al., J. Med. Chem. Lett. 24, 479, (1981); Dubowchik et al., Bioorg & Med. Chem. Lett. 8, 3347 (1998); and 60/891,028 (filed on February 21, 2007). In one aspect, the present invention relates to linkers that are useful to attach targeting groups to therapeutic agents and markers. In another aspect, the invention provides linkers that impart stability to compounds, reduce their in vivo toxicity, or otherwise favorably affect their pharmacokinetics, bioavailability and/or pharmacodynamics. It is generally preferred that in such embodiments, the linker is cleaved, releasing the active drug, once the drug is delivered to its site of action. Thus, in one embodiment of the invention, the linkers of the invention are traceless, such that once removed from the therapeutic agent or marker (such as during activation), no trace of the linker's presence remains.
In another embodiment of the invention, the linkers are characterized by their ability to be cleaved at a site in or near the target cell such as at the site of therapeutic action or marker activity. Such cleavage can be enzymatic in nature. This feature aids in reducing systemic activation of the therapeutic agent or marker, reducing toxicity and systemic side effects. Preferred cleavable groups for enzymatic cleavage include peptide bonds, ester linkages, and disulfide linkages. In other embodiments, the linkers are sensitive to pH and are cleaved through changes in pH. An important aspect of the current invention is the ability to control the speed with which the linkers cleave. Often a linker that cleaves quickly is desired. In some embodiments, however, a linker that cleaves more slowly may be preferred. For example, in a sustained release formulation or in a formulation with both a quick release and a slow release component, it may be useful to provide a linker which cleaves more slowly. WO 02/096910 provides several specific ligand-drug complexes having a hydrazine linker. However, there is no way to "tune" the linker composition dependent upon the rate of cyclization required, and the particular compounds described cleave the ligand from the drug at a slower rate than is preferred for many drug-linker conjugates. In contrast, the hydrazine linkers of the current invention provide for a range of cyclization rates, from very fast to very slow, thereby allowing for the selection of a particular hydrazine linker based on the desired rate of cyclization.
For example, very fast cyclization can be achieved with hydrazine linkers that produce a single 5-membered ring upon cleavage. Preferred cyclization rates for targeted delivery of a cytotoxic agent to cells are achieved using hydrazine linkers that produce, upon cleavage, either two 5-membered rings or a single 6-membered ring resulting from a linker having two methyls at the geminal position. The gx-w-dimethyl effect has been shown to accelerate the rate of the cyclization reaction as compared to a single 6- membered ring without the two methyls at the geminal position. This results from the strain being relieved in the ring. Sometimes, however, substitutents may slow down the reaction instead of making it faster. Often the reasons for the retardation can be traced to steric hindrance. For example, the gem dimethyl substitution allows for a much faster cyclization reaction to occur compared to when the geminal carbon is a CHi.
It is important to note, however, that in some embodiments, a linker that cleaves more slowly may be preferred. For example, in a sustained release formulation or in a formulation with both a quick release and a slow release component, it may be useful to provide a linker which cleaves more slowly. In certain embodiments, a slow rate of cyclization is achieved using a hydrazine linker that produces, upon cleavage, either a single 6-membered ring, without the gem-dimethyl substitution, or a single 7-membered ring. The linkers also serve to stabilize the therapeutic agent or marker against degradation while in circulation. This feature provides a significant benefit since such stabilization results in prolonging the circulation half-life of the attached agent or marker. The linker also serves to attenuate the activity of the attached agent or marker so that the conjugate is relatively benign while in circulation and has the desired effect, for example is toxic, after activation at the desired site of action. For therapeutic agent conjugates, this feature of the linker serves to improve the therapeutic index of the agent.
The stabilizing groups are preferably selected to limit clearance and metabolism of the therapeutic agent or marker by enzymes that may be present in blood or non-target tissue and are further selected to limit transport of the agent or marker into the cells. The stabilizing groups serve to block degradation of the agent or marker and may also act in providing other physical characteristics of the agent or marker. The stabilizing group may also improve the agent or marker's stability during storage in either a formulated or non-formulated form.
Ideally, the stabilizing group is useful to stabilize a therapeutic agent or marker if it serves to protect the agent or marker from degradation when tested by storage of the agent or marker in human blood at 37°C for 2 hours and results in less than 20%, preferably less than 10%, more preferably less than 5% and even more preferably less than 2%, cleavage of the agent or marker by the enzymes present in the human blood under the given assay conditions. The present invention also relates to conjugates containing these linkers. More particularly, the invention relates to prodrugs that may be used for the treatment of disease, especially for cancer chemotherapy. Specifically, use of the linkers described herein provide for prodrugs that display a high specificity of action, a reduced toxicity, and an improved stability in blood relative to prodrugs of similar structure.
The linkers of the present invention as described herein may be present at a variety of positions within the partner molecule.
Thus, there is provided a linker that may contain any of a variety of groups as part of its chain that will cleave in vivo, e.g., in the blood stream, at a rate which is enhanced relative to that of constructs that lack such groups. Also provided are conjugates of the linker amis with therapeutic and diagnostic agents. The linkers are useful to form prodrug analogs of therapeutic agents and to reversibly link a therapeutic or diagnostic agent to a targeting agent, a detectable label, or a solid support. The linkers may be incorporated into complexes that include a cytotoxin.
The attachment of a prodrug to an antibody may give additional safety advantages over conventional antibody conjugates of cytotoxic drugs. Activation of a prodrug may be achieved by an esterase, both within tumor cells and in several normal tissues, including plasma. The level of relevant esterase activity in humans has been shown to be very similar to that observed in rats and non-human primates, although less than that observed in mice. Activation of a prodrug may also be achieved by cleavage by glucuronidase. In addition to the cleavable peptide, hydrazine, or disulfide group, one or more self-immolative linker groups L1 are optionally introduced between the cytotoxin and the targeting agent. These linker groups may also be described as spacer groups and contain at least two reactive functional groups. Typically, one chemical functionality of the spacer group bonds to a chemical functionality of the therapeutic agent, e.g., cytotoxin, while the other chemical functionality of the spacer group is used to bond to a chemical functionality of the targeting agent or the cleavable linker. Examples of chemical functionalities of spacer groups include hydroxy, mercapto, carbonyl, carboxy, amino, ketone, and mercapto groups.
The self-immolative linkers, represented by L1, are generally a substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl or substituted or unsubstituted heteroalkyl group. In one embodiment, the alkyl or aryl groups may comprise between 1 and 20 carbon atoms. They may also comprise a polyethylene glycol moiety.
Exemplary spacer groups include, for example, 6-aminohexanol, 6- mercaptohexanol, 10-hydroxydecanoic acid, glycine and other amino acids, 1 ,6- hexanediol, β-alanine, 2-aminoethanol, cysteamine (2-aminoethanethiol), 5- aminopentanoic acid, 6-aminohexanoic acid, 3-maleimidobenzoic acid, phthalide, α- substituted phthalides, the carbonyl group, aminal esters, nucleic acids, peptides and the like.
The spacer can serve to introduce additional molecular mass and chemical functionality into the cytotoxin-targeting agent complex. Generally, the additional mass and functionality will affect the serum half-life and other properties of the complex. Thus, through careful selection of spacer groups, cytotoxin complexes with a range of serum half-lives can be produced.
The spacer(s) located directly adjacent to the drug moiety is also denoted as (L1),,,, wherein m is an integer selected from 0, 1, 2, 3, 4, 5, and 6. When multiple L1 spacers are present, either identical or different spacers may be used. L1 may be any self- immolative group.
L4 is a linker moiety that preferably imparts increased solubility or decreased aggregation properties to conjugates utilizing a linker that contains the moiety or modifies the hydrolysis rate of the conjugate. The L4 linker does not have to be self immolative. In one embodiment, the L4 moiety is substituted alkyl, unsubstituted alkyl, substituted aryl, unsubstituted aryl, substituted heteroalkyl, or unsubstituted heteroalkyl, any of which may be straight, branched, or cyclic. The substitutions may be, for example, a lower (C '-C6) alkyl, alkoxy, aklylthio, alkylamino, or dialkylamino. In certain embodiments, L comprises a non-cyclic moiety. In another embodiment, L comprises any positively or negatively charged amino acid polymer, such as polylysine or polyargenine. L4 can comprise a polymer such as a polyethylene glycol moiety. Additionally the L4 linker can comprise, for example, both a polymer component and a small chemical moiety. In a preferred embodiment, L4 comprises a polyethylene glycol (PEG) moiety.
The PEG portion of L4 may be between 1 and 50 units long. Preferably, the PEG will have 1-12 repeat units, more preferably 3-12 repeat units, more preferably 2-6 repeat units, or even more preferably 3-5 repeat units and most preferably 4 repeat units. L4 may consist solely of the PEG moiety, or it may also contain an additional substituted or unsubstituted alkyl or heteroalkyl. It is useful to combine PEG as part of the L4 moiety to enhance the water solubility of the complex. Additionally, the PEG moiety reduces the degree of aggregation that may occur during the conjugation of the drug to the antibody. In some embodiments, L comprises
Figure imgf000094_0001
directly attached to the N-terminus of (AA1 )c. R20 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl. Each R25, R25 , R26, and R26 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl; and s and t are independently integers from 1 to 6. Preferably. R"°. R 2\ R2* , R26 and R26 are hydrophobic. In some embodiments, R 20 is H or alkyl (preferably, unsubstituted lower alkyl). In some embodiments, R25, R25 , R26 and R26 are independently H or alkyl (preferably, unsubstituted C1 to C4 alkyl). In some embodiments, R23, R23 , R26 and R26 are all H. In some embodiments, t is 1 and s is 1 or 2. Peptide Linkers (F)
As discussed above, the peptidyl linkers of the invention can be represented by the general formula: (L4)p — F — (L1 )m , wherein F represents the linker portion comprising the peptidyl moiety. In one embodiment, the F portion comprises an optional additional self-immolative linker(s), L2, and a carbonyl group. In another embodiment, the F portion comprises an amino group and an optional spacer group(s), L3.
Accordingly, in one embodiment, the conjugate comprising the peptidyl linker comprises a structure of the following formula (a):
Figure imgf000095_0001
In this embodiment, L1 is a self-immolative linker, as described above, and L4 is a moiety that preferably imparts increased solubility, or decreased aggregation properties, or modifies the hydrolysis rate, as described above. L2 represents a self-immolative linker(s). In addition, m is 0, 1, 2, 3, 4, 5, or 6; and o and p are independently 0 or 1. AA1 represents one or more natural amino acids, and/or unnatural α-amino acids; c is an integer from 1 and 20. In some embodiments, c is in the range of 2 to 5 or c is 2 or 3.
In the peptide linkers of the invention of the above formula (a), AA1 is linked, at its amino terminus, either directly to L4 or, when L4 is absent, directly to the X4 group (i.e., the targeting agent, detectable label, protected reactive functional group or unprotected reactive functional group). In some embodiments, when L is present, L4 does not comprise a carboxylic acyl group directly attached to the N-terminus of (AA ')c. Thus, it is not necessary in these embodiments for there to be a carboxylic acyl unit directly between either L4 or X4 and AA1, as is necessary in the peptidic linkers of U.S. Patent No. 6,214,345.
In another embodiment, the conjugate comprising the peptidyl linker comprises a structure of the following formula (b):
Figure imgf000095_0002
In this embodiment, L4 is a moiety that preferably imparts increased solubility, or decreased aggregation properties, or modifies the hydrolysis rate, as described above; L3 is a spacer group comprising a primary or secondary amine or a carboxyl functional group, and either the amine of L3 forms an amide bond with a pendant carboxyl functional group of D or the carboxyl of L3 forms an amide bond with a pendant amine functional group of D; and o and p are independently 0 or 1. AA1 represents one or more natural amino acids, and/or unnatural α-amino acids; c is an integer from 1 and 20. In this embodiment, L is absent (i.e., m is 0 in the general formula).
In the peptide linkers of the invention of the above formula (b), AA is linked, at its amino terminus, either directly to L4 or, when L4 is absent, directly to the X4 group (i.e., the targeting agent, detectable label, protected reactive functional group or unprotected reactive functional group). In some embodiments, when L4 is present, L4 does not comprise a carboxylic acyl group directly attached to the N-terminus of (AA ')c. Thus, it is not necessary in these embodiments for there to be a carboxylic acyl unit directly between either L4 or X4 and AA1, as is necessary in the peptidic linkers of U.S. Patent No. 6,214,345.
The Self-Immolative Linker L~
The self-immolative linker L2 is a bifunctional chemical moiety which is capable of covalently linking together two spaced chemical moieties into a normally stable tripartate molecule, releasing one of said spaced chemical moieties from the tripartate molecule by means of enzymatic cleavage; and following said enzymatic cleavage, spontaneously cleaving from the remainder of the molecule to release the other of said spaced chemical moieties. In accordance with the present invention, the self-immolative spacer is covalently linked at one of its ends to the peptide moiety and covalently linked at its other end to the chemically reactive site of the drug moiety whose derivatization inhibits pharmacological activity, so as to space and covalently link together the peptide moiety and the drug moiety into a tripartate molecule which is stable and pharmacologically inactive in the absence of the target enzyme, but which is enzymatically cleavable by such target enzyme at the bond covalently linking the spacer moiety and the peptide moiety to thereby affect release of the peptide moiety from the tripartate molecule. Such enzymatic cleavage, in turn, will activate the self-immolating character of the spacer moiety and initiate spontaneous cleavage of the bond covalently linking the spacer moiety to the drug moiety, to thereby affect release of the drug in pharmacologically active form.
The self-immolative linker L2 may be any self-immolative group. Preferably L2 is a substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, unsubstituted heterocycloalkyl, substituted heterocycloalkyl, substituted and unsubstituted aryl, and substituted and unsubstituted heteroaryl.
One particularly preferred seif-immolative spacer L2 may be represented by the formula (c):
Figure imgf000097_0001
The aromatic ring of the aminobenzyl group may be substituted with one or more "K" groups. A "K" group is a substituent on the aromatic ring that replaces a hydrogen otherwise attached to one of the four non-substituted carbons that are part of the ring structure. The "K" group may be a single atom, such as a halogen, or may be a multi- atom group, such as alkyl, heteroalkyl, amino, nitro, hydroxy, alkoxy, haloalkyl, and cyano. Each K is independently selected from the group consisting of substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO2, NR21R22, NR21COR22, OCONR21R22, OCOR21, and OR21, wherein R21 and R22 are independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl and unsubstituted heterocycloalkyl. Exemplary K substituents include, but are not limited to, F, Cl, Br, I, NO2, OH, OCH3, NHCOCH3, N(CHj)2, NHCOCF3 and methyl. For "K1", / is an integer of O, 1 , 2, 3, or 4. In one preferred embodiment, / is O.
The ether oxygen atom of the structure shown above is connected to a carbonyl group. The line from the NR24 functionality into the aromatic ring indicates that the amine functionality may be bonded to any of the five carbons that both form the ring and are not substituted by the -CH2-O- group. Preferably, the NR24 functionality of X is covalently bound to the aromatic ring at the para position relative to the -CH2-O- group. R24 is a member selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl. In a specific embodiment, R24 is hydrogen.
In one embodiment, the invention provides a peptide linker of formula (a) above, wherein F comprises the structure:
Figure imgf000098_0001
where R24 is selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl. Each K is a member independently selected from the group consisting of substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO2, NR21R22, NR21COR22, OCONR21R22, OCOR21, and OR21 where R21 and R22 are independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl; and / is an integer of 0,1, 2, 3, or 4.
In another embodiment, the peptide linker of formula (a) above comprises a -F- (L1),,,- that comprises the structure:
Figure imgf000098_0002
where each R » 2"4 i •s a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl.
In some embodiments, the self-immolative spacer L1 or L" includes
Figure imgf000099_0001
where each R17, R18, and R19 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryl, and w is an integer from 0 to 4. In some embodiments, R17 and R18 are independently H or alkyl (preferably, unsubstituted C 1-4 alkyl). Preferably, R17 and R are Cl -4 alkyl, such as methyl or ethyl. In some embodiments, w is 0. While not wishing to be bound to any particular theory, it has been found experimentally that this particular self-immolative spacer cyclizes relatively quickly. In some embodiments, L1 or L2 includes
Figure imgf000099_0002
The Spacer Group V The spacer group LJ is characterized in that it comprises a primary or secondary amine or a carboxyl functional group, and either the amine of the L group forms an amide bond with a pendant carboxyl functional group of D or the carboxyl of LJ forms an amide bond with a pendant amine functional group of D. L3 can be selected from the group consisting of substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted hteroaryl, or substituted or unsubstituted heterocycloalkyl. In a preferred embodiment, L3 comprises an aromatic group. More preferably, L3 comprises a benzoic acid group, an aniline group or indole group. Non-limiting examples of structures that can serve as an -L3 -NH- spacer include the following structures:
Figure imgf000100_0001
where Z is a member selected from O, S and NR23, and where R23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl.
Upon cleavage of the linker of the invention containing L3, the IS moiety remains attached to the drug, D. Accordingly, the L/ moiety is chosen such that its presence attached to D does not significantly alter the activity of D. In another embodiment, a portion of the drug D itself functions as the L3 spacer. For example, in one embodiment, the drug, D, is a duocarmycin derivative in which a portion of the drug functions as the L spacer Non-hmiting examples of such embodiments include those in which NHo- (L3)-D has a structure selected fiom the group consisting of
Figure imgf000101_0001
where Z is a member selected fiom O, S and NR2\ where R2^ is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl; and where the NH2 group on each structure reacts with (AA ')c to form -(AA')C-NH-.
The Peptide Sequence AA The group AA1 represents a single amino acid or a plurality of ammo acids that are joined together by amide bonds. The amino acids may be natural amino acids and/or unnatui al α-amino acids
The peptide sequence (AA')cis functionally the amidifϊcation residue of a single ammo acid (when c=l) oi a plurality of ammo acids joined together by amide bonds The peptide of the current invention is selected for directing enzyme-catalyzed cleavage of the peptide by an enzyme in a location of interest in a biological system. For example, for conjugates that are targeted to a cell using a targeting agent, but not internalized by that cell, a peptide is chosen that is cleaved by one or more proteases that may exist in the extracellular matrix, e.g., due to release of the cellular contents of nearby dying cells, such that the peptide is cleaved extracellularly. The number of amino acids within the peptide can range from 1 to 20; but more preferably there will be 1-8 amino acids, 1-6 amino acids or 1, 2, 3 or 4 amino acids comprising (AA ')c. Peptide sequences that are susceptible to cleavage by specific enzymes or classes of enzymes are well known in the art. Many peptide sequences that are cleaved by enzymes in the serum, liver, gut, etc. are known in the art. An exemplary peptide sequence of the invention includes a peptide sequence that is cleaved by a protease. The focus of the discussion that follows on the use of a protease-sensitive sequence is for clarity of illustration and does not serve to limit the scope of the present invention. When the enzyme that cleaves the peptide is a protease, the linker generally includes a peptide containing a cleavage recognition sequence for the protease. A cleavage recognition sequence for a protease is a specific amino acid sequence recognized by the protease during proteolytic cleavage. Many protease cleavage sites are known in the art, and these and other cleavage sites can be included in the linker moiety. See, e.g., Matayoshi et al. Science 2Al \ 954 ( 1990); Dunn et al. Meth. Enzymol. 241 : 254 (1994); Seidah et al. Meth. Enzymol. 244: 175 (1994); Thomberry, Meth. Enzymol. 244: 615 (1994); Weber et al. Meth. Enzymol. 244: 595 (1994); Smith et al. Meth. Enzymol. 244: 412 (1994); Bouvier et al. Meth. Enzymol. 248: 614 (1995), Hardy et al, in Amyloid Protein Precursor in Development, Aging, and Alzheimer's Disease, ed. Masters et al. pp. 190-198 (1994).
The amino acids of the peptide sequence (AA')C are chosen based on their suitability for selective enzymatic cleavage by particular molecules such as tumor- associated protease. The amino acids used may be natural or unnatural amino acids. They may be in the L or the D configuration. In one embodiment, at least three different amino acids are used. In another embodiment, only two amino acids are used. In a preferred embodiment, the peptide sequence (AA')C is chosen based on its ability to be cleaved by a lysosomal proteases, non-limiting examples of which include cathepsins B, C, D, H, L and S. Preferably, the peptide sequence (AA1 )c is capable of being cleaved by cathepsin B in vitro, which can be tested using in vitro protease cleavage assays known in the art.
In another embodiment, the peptide sequence (AA ')c is chosen based on its ability to be cleaved by a tumor-associated protease, such as a protease that is found extracellularly in the vicinity of tumor cells, non-limiting examples of which include thimet oligopeptidase (TOP) and CD 10. The ability of a peptide to be cleaved by TOP or CD 10 can be tested using in vitro protease cleavage assays known in the art.
Suitable, but non-limiting, examples of peptide sequences suitable for use in the conjugates of the invention include Val-Cit, Cit-Cit, Val-Lys, Phe-Lys, Lys-Lys, AIa- Lys, Phe-Cit, Leu-Cit, Ile-Cit, Tip, Gt, Phe-Ala, Phe-N9-tosyl-Arg, Phe-N9-nitro-Arg, Phe-Phe-Lys, D-Phe-Phe-Lys, Gly-Phe-Lys, Leu-Ala-Leu, Ile-Ala-Leu, Val-Ala-Val, Ala-Leu- Ala-Leu (SEQ ID NO:88), β-Ala-Leu- Ala-Leu (SEQ ID NO:89), Gly-Phe-Leu- GIy (SEQ ID NO:90), Val-Ala, Leu-Leu-Gly-Leu (SEQ ID NO: 101), Leu-Asn-Ala, and Lys-Leu-Val. Preferred peptides sequences are Val-Cit and Val-Lys.
In another embodiment, the amino acid located the closest to the drug moiety is selected from the group consisting of: Ala, Asn, Asp, Cit, Cys, GIn, GIu, GIy, He, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and VaL In yet another embodiment, the amino acid located the closest to the drug moiety is selected from the group consisting of: Ala, Asn, Asp, Cys, GIn, GIu, GIy, He, Leu, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and VaL
Proteases have been implicated in cancer metastasis. Increased synthesis of the protease urokinase was correlated with an increased ability to metastasize in many cancers. Urokinase activates plasmin from plasminogen, which is ubiquitously located in the extracellular space and its activation can cause the degradation of the proteins in the extracellular matrix through which the metastasizing tumor cells invade. Plasmin can also activate the collagenases thus promoting the degradation of the collagen in the basement membrane surrounding the capillaries and lymph system thereby allowing tumor cells to invade into the target tissues (Dano, et al. Adv. Cancer. Res., 44: 139 (1985)). Thus, it is within the scope of the present invention to utilize as a linker a peptide sequence that is cleaved by urokinase.
The invention also provides the use of peptide sequences that are sensitive to cleavage by tryptases. Human mast cells express at least four distinct tryptases. designated α βl, βlf, and βlll. These enzymes are not controlled by blood plasma proteinase inhibitors and only cleave a few physiological substrates in vitro. The tryptase family of serine proteases has been implicated in a variety of allergic and inflammatory diseases involving mast cells because of elevated tryptase levels found in biological fluids from patients with these disorders. However, the exact role of tryptase in the pathophysiology of disease remains to be delineated. The scope of biological functions and coiτesponding physiological consequences of tryptase are substantially defined by their substrate specificity.
Tryptase is a potent activator of pro-urokinase plasminogen activator (uPA) the zymogen form of a protease associated with tumor metastasis and invasion. Activation of the plasminogen cascade, resulting in the destruction of extracellular matrix for cellular extravasation and migration, may be a function of tryptase activation of pro-urokinase plasminogen activator at the P4-P1 sequence of Pro-Arg-Phe-Lys (SEQ ID NO:91) (Stack, et al., Journal of Biological Chemistry 269 (13): 9416-9419 (1994)). Vasoactive intestinal peptide, a neuropeptide that is implicated in the regulation of vascular permeability, is also cleaved by tryptase, primarily at the Thr-Arg-Leu-Arg (SEQ ID NO:92) sequence (Tarn, et al., Am. J. Respir. Cell MoL Biol. 3: 27-32 (1990)). The G- protein coupled receptor PAR-2 can be cleaved and activated by tryptase at the Ser-Lys- GIy- Arg (SEQ ID NO:93) sequence to drive fibroblast proliferation, whereas the thrombin activated receptor PAR-I is inactivated by tryptase at the Pro-Asn-Asp-Lys (SEQ ID NO:94) sequence (Molino et al., Journal of Biological Chemistry 272(7): 4043- 4049 (1997)). Taken together, this evidence suggests a central role for tryptase in tissue remodeling as a consequence of disease. This is consistent with the profound changes observed in several mast cell-mediated disorders. One hallmark of chronic asthma and other long-term respiratory diseases is fibrosis and thickening of the underlying tissues that could be the result of tryptase activation of its physiological targets. Similarly, a series of reports have shown angiogenesis to be associated with mast cell density, tryptase activity and poor prognosis in a variety of cancers (Coussens et al., Genes and Development 13(1 1): 1382-97 (1999)); Takanami et al., Cancer 88(12): 2686-92 (2000); Toth-Jakatics et al, Human Pathology 31(8): 955-960 (2000); Ribatti et al, International Journal of Cancer 85(2): 171-5 (2000)). Methods are known in the art for evaluating whether a particular protease cleaves a selected peptide sequence. For example, the use of 7-amino-4-methyl coumarin (AMC) fluorogenic peptide substrates is a well-established method for the determination of protease specificity (Zimmerman, M., et al., (1977) Analytical Biochemistry 78:47-51). Specific cleavage of the anilide bond liberates the fluorogenic AMC leaving group allowing for the simple determination of cleavage rates for individual substrates. More recently, arrays (Lee, D., et al., (1999) Bioorganic and Medicinal Chemistry Letters 9: 1667-72) and positional-scanning libraries (Rano, T.A., et al., (1997) Chemistry and Biology 4: 149-55) of AMC peptide substrate libraries have been employed to rapidly profile the N-terminal specificity of proteases by sampling a wide range of substrates in a single experiment. Thus, one of skill in the art may readily evaluate an array of peptide sequences to determine their utility in the present invention without resort to undue experimentation.
The antibody-partner conjugate of the current invention may optionally contain two or more linkers. These linkers may be the same or different. For example, a peptidyl linker may be used to connect the drug to the ligand and a second peptidyl linker may attach a diagnostic agent to the complex. Other uses for additional linkers include linking analytical agents, biomolecules, targeting agents, and detectable labels to the antibody- partner complex.
Also within the scope of the present invention are compounds of the invention that are poly- or multi-valent species, including, for example, species such as dimers, trimers, tetramers and higher homologs of the compounds of the invention or reactive analogues thereof. The poly- and multi-valent species can be assembled from a single species or more than one species of the invention. For example, a dimeric construct can be "homo-dimeric" or 'lieterodimeric.''' Moreover, poly- and multi-valent constructs in which a compound of the invention or a reactive analogue thereof, is attached to an oligomeric or polymeric framework (e.g., polylysine, dextran, hydroxyethyl starch and the like) are within the scope of the present invention. The framework is preferably polyfunctional (i.e. having an array of reactive sites for attaching compounds of the invention). Moreover, the framework can be derivatized with a single species of the invention or more than one species of the invention. Moreover, the present invention includes compounds that are functionalized to afford compounds having water-solubility that is enhanced relative to analogous compounds that are not similarly functionalized. Thus, any of the substituents set forth herein can be replaced with analogous radicals that have enhanced water solubility. For example, it is within the scope of the invention to, for example, replace a hydroxyl group with a diol, or an amine with a quaternary amine, hydroxy amine or similar more water- soluble moiety. In a preferred embodiment, additional water solubility is imparted by substitution at a site not essential for the activity towards the ion channel of the compounds set forth herein with a moiety that enhances the water solubility of the parent compounds. Methods of enhancing the water-solubility of organic compounds are known in the art. Such methods include, but are not limited to, functionalizing an organic nucleus with a permanently charged moiety, e.g., quaternary ammonium, or a group that is charged at a physiologically relevant pH, e.g. carboxylic acid, amine. Other methods include, appending to the organic nucleus hydroxyl- or amine-containing groups, e.g. alcohols, polyols, polyethers, and the like. Representative examples include, but are not limited to, polylysine, polyethyleneimine, poly(ethyleneglycol) and poly(propyleneglycol). Suitable functionalization chemistries and strategies for these compounds are known in the art. See, for example, Dunn, R.L., et al., Eds. POLYMERIC DRUGS AND DRUG DELIVERY SYSTEMS, ACS Symposium Series Vol. 469, American Chemical Society, Washington, D.C. 1991. Hydrazine Linkers (H)
In a second embodiment, the conjugate of the invention comprises a hydrazine self-immolative linker, wherein the conjugate has the structure:
X4 (L4)p-H-(L1)m D wherein D, L I , r L 4 , and X are as defined above and described further herein, and H is a linker comprising the structure:
Figure imgf000107_0001
wherein nj is an integer from 1 - 10; n2 is 0, 1, or 2; each R24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl; and I is either a bond (i.e., the bond between the carbon of the backbone and the adjacent nitrogen) or:
Figure imgf000107_0002
wherein n3 is 0 or 1 , with the proviso that when n^ is 0, n2 is not 0; and 114 is I, 2, or 3, wherein when I is a bond, ni is 3 and n2 is 1 , D can not be
Figure imgf000107_0003
where R is Me or CH2- CH2-NMe2.
In one embodiment, the substitution on the phenyl ring is a para substitution. In preferred embodiments, n> is 2, 3, or 4 or ni is 3. In preferred embodiments, n2 is 1. In preferred embodiments, I is a bond (i.e., the bond between the carbon of the backbone and the adjacent nitrogen). In one aspect, the hydrazine linker, H, can form a 6- membered self immolative linker upon cleavage, for example, when n3 is 0 and 114 is 2. In another aspect, the hydrazine linker, H, can form two 5-membered self immolative linkers upon cleavage. In yet other aspects, H forms a 5-membered self immolative linker, H forms a 7-membered self immolative linker, or H forms a 5-membered self immolative linker and a 6-membered self immolative linker, upon cleavage. The rate of cleavage is affected by the size of the ring formed upon cleavage. Thus, depending upon the rate of cleavage desired, an appropriate size ring to be formed upon cleavage can be selected.
Five Membered Hydrazine Linkers
In one embodiment, the hydrazine linker comprises a 5-membered hydrazine linker, wherein H comprises the structure:
Figure imgf000108_0001
In a preferred embodiment, ni is 2, 3, or 4. In another preferred embodiment, ni is 3.
In the above structure, each R24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl. In one embodiment, each R24 is independently H or a Ci - C6 alkyl. In another embodiment, each R24 is independently H or a Ci - C3 alkyl, more preferably H or CH3. In another embodiment, at least one R24 is a methyl group. In another embodiment, each R24 is H. Each R24 is selected to tailor the compounds steric effects and for altering solubility. The 5-membered hydrazine linkers can undergo one or more cyclization reactions that separate the drug from the linker, and can be described, for example, by:
Figure imgf000108_0002
An exemplary synthetic route for preparing a five membered linker of the invention is:
Figure imgf000109_0001
a b C
H
Boc^
EDC N H
Figure imgf000109_0002
The Cbz-protected DMDA b is reacted with 2,2-Dimethyl-makmic acid a in solution vvitli thionyl chloride to form a Cbz-DMDA-2,2-dimethylmalonic acid c. Compound c is reacted with Boc-N-methyl hydrazine d in the presence of EDC to form DMDA-2,2- dimetylmalonic-Boc-N-methylhydrazine e.
Six Membered Hydrazine Linkers
In another embodiment, the hydrazine linker comprises a 6-membered hydrazine linker, wherein H comprises the structure:
Figure imgf000109_0003
In a pre ferred embodiment, 1I1 is 3. In the above structure, each R24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl. In one embodiment, each R24 is independently H or a Ci - Ce alkyl. In another embodiment, each R24 is independently H or a Ct - C3 alkyl, more preferably H or CH3. In another embodiment, at least one R" is a methyl group. In another embodiment, each R24 is H. Each R"4 is selected to tailor the compounds steric effects and for altering solubility. In a preferred embodiment, H comprises the structure:
Figure imgf000110_0001
In one embodiment, H comprises a geminal dimethyl substitution. In one embodiment of the above structure, each R24 is independently an H or a substituted or unsubstituted alkyl.
The 6-membered hydrazine linkers will undergo a cyclization reaction that separates the drug from the linker, and can be described as:
Figure imgf000110_0002
An exemplary synthetic route for preparing a six membered linker of the invention is:
Figure imgf000110_0003
The Cbz-protected dimethyl alanine a in solution with dichlormethane, was reacted with HOAt, and CPI to form a Cbz-protected dimethylalanine hydrazine b. The hydrazine b is deprotected by the action of methanol, forming compound c.
Other Hydrazine Linkers
It is contemplated that the invention comprises a linker having seven members. This linker would likely not cyclize as quickly as the five or six membered linkers, but this may be preferred for some antibody-partner conjugates. Similarly, the hydrazine linker may comprise two six membered rings or a hydrazine linker having one six and one five membered cyclization products. A five and seven membered linker as well as a six and seven membered linker are also contemplated. Another hydrazine structure, H, has the formula:
Figure imgf000111_0001
where q is 0, 1,2, 3, 4, 5, or 6; and each R24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl. This hydrazine structure can also form five-, six-, or seven-membered rings and additional components can be added to form multiple rings. Disulfide Linkers (J)
In yet another embodiment, the linker comprises an enzymatically cleavable disulfide group. In one embodiment, the invention provides a cytotoxic antibody-partner compound having a structure according to Formula (d):
Figure imgf000111_0002
wherein D, L1, L4, and X4 are as defined above and described further herein, and J is a disulfide linker comprising a group having the structure:
wherein each R24 is a member independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, and unsubstituted heteroalkyl; each K is a member independently selected from the group consisting of substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO2, NR21R22, NR21COR22, OCONR21R22, OCOR21, and OR21 wherein R21 and R22 are independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl and unsubstituted heterocycloalkyl; / is an integer of 0,1, 2, 3, or 4; and d is an integer of O, 1, 2, 3, 4, 5, or 6.
The aromatic ring of the disulfides linker may be substituted with one or more "K" groups. A "K" group is a substituent on the aromatic ring that replaces a hydrogen otherwise attached to one of the four non-substituted carbons that are part of the ring structure. The "K" group may be a single atom, such as a halogen, or may be a multi- atom group, such as alkyl, heteroalkyl, amino, nitro, hydroxy, alkoxy, haloalkyl, and cyano. Exemplary K substituents independently include, but are not limited to, F, Cl, Br, I, NO2, OH, OCH3, NHCOCH3, N(CHj)2, NHCOCF., and methyl. For "K1", / is an integer of O, 1 , 2, 3, or 4. In a specific embodiment, / is O.
In a preferred embodiment, the linker comprises an enzymatically cleavable disulfide group of the following formula:
Figure imgf000112_0001
In this embodiment, the identities of L4, X4, p, and R24 are as described above, and d is O, 1, 2, 3, 4, 5, or 6. In a particular embodiment, d is 1 or 2.
A more specific disulfide linker is shown in the formula below:
Figure imgf000113_0001
A specific example of this embodiment is as follows:
Figure imgf000113_0002
Preferably, d is 1 or 2.
Another disulfide linker is shown in the formula below:
Figure imgf000113_0003
A specific example of this embodiment is as follows:
Figure imgf000113_0004
-1 Preferably, d is 1 or 2.
In various embodiments, the disulfides are ortho to the amine. In another specific embodiment, a is 0. In preferred embodiments, R24 is independently selected from H and CH3.
An exemplary synthetic route for preparing a disulfide linker of the invention is as follows:
Figure imgf000114_0001
A solution of 3-mercaptopropionic acid a is reacted with aldrithiol-2 to form 3- methyl benzothiazolium iodide b. 3-methylbenzothiazolium iodide c is reacted with sodium hydroxide to form compound d. A solution of compound d with methanol is further reacted with compound b to form compound e. Compound e deprotected by the action of acetyl chloride and methanol forming compound f.
For further discussion of types of cytotoxins, linkers and other methods for conjugating therapeutic agents to antibodies, see also PCT Publication WO 2007/059404 to Gangwar et al. and entitled "Cytotoxic Compounds And Conjugates," Saito, G. et al.
(2003) Adv. Drug Deliv. Rev. 55: 199-215; Trail, P.A. et al. (2003) Cancer Immunol.
Immunother. 52:328-337; Payne, G. (2003) Cancer Cell 3:207-212; Allen, T.M. (2002)
Nat. Rev. Cancer 2:750-763; Pastan, I. and Kreitman, R. J. (2002) Curr. Opin. Investig. Drugs 3: 1089-1091 ; Senter, P.D. and Springer, CJ. (2001 ) Adv. Drug Deliv. Rev.
53:247-264, each of which is hereby incorporated by reference in their entirety. Partner Molecules
In one aspect, the present invention features an antibody conjugated to a partner molecule, such as a cytotoxin, a drug (e.g., an immunosuppressant) or a radiotoxin. Such conjugates are also referred to herein as "immunoconjugates." Immunoconjugates that include one or more cytotoxins are referred to as "immunocytotoxins." A cytotoxin or cytotoxic agent includes any agent that is detrimental to (e.g., kills) cells.
Examples of partner molecules of the present invention include taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1 -dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof. Examples of partner molecules also include, for example, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5- fluorouracil decarbazine), alkylating agents (e.g., mechlorethamine, thioepa chlorambucil, melphalan, carmustine (BSNU) and lomustine (CCNU), cyclothosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cis- dichlorodiamine platinum (II) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin), bleomycin, mithramycin, and anthramycin (AMC)), and anti-mitotic agents (e.g., vincristine and vinblastine).
Other preferred examples of partner molecules that can be conjugated to an antibody of the invention include duocarmycins, calicheamicins, maytansines and auristatins, and derivatives thereof. An example of a calicheamicin antibody conjugate is commercially available (Mylotarg©; American Home Products). Preferred examples of partner molecule are CC- 1065 and the duocarmycins. CC-
1065 was first isolated from Streptomyces zelensis in 1981 by the Upjohn Company (Hanka et al, J. Antibiot. 31 : 121 1 (1978); Martin et al, J. Antibiot. 33: 902 ( 1980); Martin et al., J. Antibiot. 34: 1 1 19 ( 1981 )) and was found to have potent antitumor and antimicrobial activity both in vitro and in experimental animals (Li et al., Cancer Res. 42: 999 (1982)). CC- 1065 binds to double-stranded B-DNA within the minor groove (Swenson et al, Cancer Res. 42: 2821 (1982)) with the sequence preference of 5'- d(A/GNTTA)-3' and 5'-d(AAAAA)-3' and alkylates the N3 position of the 3'-adenine by its CPI left-hand unit present in the molecule (Hurley et al., Science 226: 843 (1984)). Despite its potent and broad antitumor activity, CC- 1065 cannot be used in humans because it causes delayed death in experimental animals.
Many analogues and derivatives of CC- 1065 and the duocarmycins are known in the art. The research into the structure, synthesis and properties of many of the compounds has been reviewed. See, for example, Boger et al., Angew. Chem. Int. Ed. Engl. 35: 1438 (1996); and Boger et al., Chem. Rev. 97: 787 (1997). A group at Kyowa Hakko Kogya Co., Ltd. has prepared a number of CC- 1065 derivatives. See, for example, U.S. Pat. No. 5, 101, 038; 5,641,780; 5,187,186; 5,070,092; 5,703,080; 5,070,092; 5,641,780; 5, 101,038; and 5,084,468; and published PCT application, WO 96/10405 and published European application 0 537 575 Al .
The Upjohn Company (Pharmacia Upjohn) has also been active in preparing derivatives of CC- 1065. See, for example, U.S. Patent No. 5,739,350; 4,978,757, 5,332, 837 and 4,912,227.
A particularly preferred aspect of the current invention provides a cytotoxic compound having a structure according to the following formula (e):
Figure imgf000116_0001
in which ring system A is a member selected from substituted or unsubstituted aryl substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups. Exemplary ring systems include phenyl and pyrrole.
The symbols E and G are independently selected from H, substituted or unsubstituted alkyl, substituted or unsubslituted heteroalkyl, a heteroatom, a single bond or E and G are optionally joined to form a ring system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl.
The symbol X represents a member selected from O, S and NR23. R23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl.
The symbol R3 represents a member selected from (=0), SR1 ', NHR1 ' and OR1 ', in which R1 ' is H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, monophosphates, diphosphates, triphosphates, sulfonates, acyl, C(O)R12 R13, C(O)OR12, C(O)NR12R13, P(O)(ORI2)2, C(O)CHR12R13, SR12 or SiR12R13R14. The symbols R12, R13, and R14 independently represent H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryl, where R12 and R13 together with the nitrogen or carbon atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms. One or more of R12, Rl\ or R14 can include a cleavable group within its structure.
R4, R4\ R5 and R5' are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, halogen, NO2, NR15R16, NC(O)R15, OC(O)NR15R16, OC(O)OR15, C(O)R15, SR15, OR15, CR15^NR16, and
O(CH2)nN(CH3)2, where n is an integer from 1 to 20, or any adjacent pair of R4, R4', R3 and R5\ together with the carbon atoms to which they are attached, are joined to form a substituted or unsubstituted cycloalkyl or heterocycloalkyl ring system having from 4 to 6 members. R15 and R16 independently represent H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl and substituted or unsubstituted peptidyl, where R15 and R16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms. One exemplary structure is aniline. R4, R4\ R5, R5\ R1 ', R12, R13, R15 and R16 optionally contain one or more cleavable groups within their structure, such as a cleavable linker or cleavable substrate. Exemplary cleavable groups include, but are not limited to peptides, amino acids, hydrazines, disulfides, and cephalosporin derivatives. In some embodiments, at least one of R4, R4', R\ R5\ R1 1, R12, R13, R15 and R16 is used to join the drug to a linker or enzyme cleavable substrate of the present invention, as described herein, for example to L1, if present or to F, H, J, or X", or J.
In a still further exemplary embodiment, at least one of R4, R4\ R5, R5', R11, R12, R13, R15 and R16 bears a reactive group appropriate for conjugating the compound. In a further exemplary embodiment, R4, R4', R5, R5', R1 ', R12, R13, R15 and R16 are independently selected from H, substituted alkyl and substituted heteroalkyl and have a reactive functional group at the free terminus of the alkyl or heteroalkyl moiety. One or more of R4, R4', R5, R5', R1 ', R12, R13, R15 and R16 may be conjugated to another species, e.g, targeting agent, detectable label, solid support, etc. R6 is a single bond which is either present or absent. When R6 is present, R6 and
R7 are joined to form a cyclopropyl ring. R7 is CH2-X1 or -CH2-. When R7 is -CH2- it is a component of the cyclopropane ring. The symbol X1 represents a leaving group such as a halogen, for example Cl, Br or F. The combinations of R6 and R7 are interpreted in a manner that does not violate the principles of chemical valence. X1 may be any leaving group. Useful leaving groups include, but are not limited to, halogens, azides, sulfonic esters (e.g., alkylsulfonyl, arylsulfonyl), oxonium ions, alkyl perchlorates, ammonioalkanesulfonate esters, alkylfluorosulfonates and fluorinated compounds (e.g., triflates, nonaflates, tresylates) and the like. Particular halogens useful as leaving groups are F, Cl and Br. The choice of these and other leaving groups appropriate for a particular set of reaction conditions is within the abilities of those of skill in the art (see, for example, March J, Advanced Organic Chemistry, 2nd Edition, John Wiley and Sons, 1992; Sandler SR, Karo W, Organic Functional Group Preparations, 2nd Edition, Academic Press, Inc., 1983; and Wade LG, Compendium of Organic Synthetic Methods, John Wiley and Sons, 1980). The curved line within the six-membered ring indicates that the ring may have one or more degrees of unsaturation, and it may be aromatic. Thus, ring structures such as those set forth below, and related structures, are within the scope of Formula (f):
Figure imgf000119_0001
In some embodiments, at least one of R , R '. R3, and R5' links said drug to L , if present, or to F, H, J, or X2, and includes
Figure imgf000119_0002
where v is an integer from 1 to 6; and each R27, R27 , R28, and R28 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl. In some embodiments, R27, R27 , R2S, and R*" are all H. In some embodiments, v is an integer from 1 to 3 (preferably, 1 ). This unit can be used to separate aryl substituents from the drug and thereby resist or avoid generating compounds that are substrates for multi-drug resistance.
In one embodiment, R1 ' includes a moiety, X5, that does not self-cyclize and links the drug to L1, if present, or to F, H, J, or X2. The moiety, X\ is preferably cleavable using an enzyme and, when cleaved, provides the active drug. As an example, R can have the following structure (with the right side coupling to the remainder of the drug):
Figure imgf000119_0003
In an exemplary embodiment, ring system A of fonnula (e) is a substituted or unsubstituted phenyl ring. Ring system A may be substituted with one or more aryl group substituents as set forth in the definitions section herein. In some embodiments, the phenyl ring is substituted with a CN or methoxy moiety.
In some embodiments, at least one of R4, R4', R5, and R3' links said drug to L1, if present, or to F, H, J, or X2, and R3 is selected from SR1 1, NHR1 1 and OR". R1 1 is selected from -SO(OH)2, -PO(OH)2, -AAn, -Si(CH3)2C(CH3)3, -C(O)OPhNH(AA)1n,
Figure imgf000120_0001
Figure imgf000121_0001
other sugar or combination of sugars,
Figure imgf000121_0002
and pharmaceutically acceptable salts thereof, where n is any integer in the range of 1 to 10, m is any integer in the range of 1 to 4, p is any integer in the range of 1 to 6, and AA is any natural or non-natural amino acid. In some embodiments, AAn or AA1n is selected from the same amino acid sequences described above for the peptide linkers (F) and optionally is the same as the amino acid sequence used in the linker portion of R4, R4', R , or R '. In at least some embodiments, R" is cleavable in vivo to provide an active drug compound. In at least some embodiments, R3 increases in vivo solublility of the compound. In some embodiments, the rate of decrease of the concentration of the active drug in the blood is substantially faster than the rate of cleavage of R' to provide the active drug. This may be particularly useful where the toxicity of the active drag is substantially higher than that of the prodrug foπn. In other embodiments, the rate of cleavage of R" to provide the active drug is faster than the rate of decrease of concentration of the active drug in the blood.
In another exemplary embodiment, the invention provides a compound having a structure according to Formula (g):
Figure imgf000122_0001
In this embodiment, the identities of the substituents R3, R4, R4', R5, R5\ R6, R7 and X are substantially as described above for Formula (a), as well as preferences for particular embodiments. The symbol Z is a member independently selected from O, S and NR"J. The symbol R23 represents a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl. Each R23 is independently selected. The symbol R1 represents H, substituted or unsubstituted lower alkyl, or C(O)R8 or CO2R8. R8 is a member selected from substituted alkyl, unsubstituted alkyl, NR9R10, NR9NHR10 and OR9. R9 and R10 are independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl. R2 is H, or substituted or unsubstituted lower alkyl. It is generally preferred that when R2 is substituted alkyl, it is other than a perfluoroalkyl, e.g., CF3. In one embodiment, R2 is a substituted alkyl wherein the substitution is not a halogen. In another embodiment, R2 is an unsubstituted alkyl.
In some embodiments R1 is an ester moiety, such as CO2CH3. In some embodiments, R2 is a lower alkyl group, which may be substituted or unsubstituted. A presently preferred lower alkyl group is CH3. In some preferred embodiments, R1 is CO2CH3 and R2 is CH3.
In some embodiments, R4, R4', R5, and R5' are members independently selected from H, halogen, NH2, OMe, O(CH2)2N(R29)2 and NO2. Each R2q is independently H or lower alkyl (e.g., methyl).
In some embodiments, the drug is selected such that the leaving group X1 is a member selected from the group consisting of halogen, alkylsulfonyl, arylsulfonyl, and azide. In some embodiments, X1 is F, Cl, or Br.
In some embodiments, Z is O or NH. In some embodiments, X is O.
In yet another exemplary embodiment, the invention provides compounds having a structure according to Formula (h) or (i):
(h) (i)
Another preferred structure of the duocarmycin analog of Formula (e) is a structure in which the ring system A is an unsubstituted or substituted phenyl ring. The preferred substituents on the drug molecule described hereinabove for the structure of Formula 7 when the ring system A is a pyrrole are also preferred substituents when the ring system A is an unsubstituted or substituted phenyl ring.
For example, in a preferred embodiment, the drug (D) comprises a structure (j):
Figure imgf000124_0001
In this structure, R3, R6, R7, X are as described above for Formula (e). Furthermore, Z is a member selected from O, S and NR23, wherein R23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl;
R1 is H, substituted or unsubstituted lower alkyl, C(O)R8, or CO2R8, wherein R8 is a member selected from NR9R10 and OR9, in which R9 and R10 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl;
R1 is H, substituted or unsubstituted lower alkyl, or C(O)R8, wherein R8 is a member selected from NR R1 and OR , in which Ry and R10 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl;
R" is H, or substituted or unsubstituted lower alkyl or unsubstituted heteroalkyl or cyano or alkoxy; and R2 is H, or substituted or unsubstituted lower alkyl or unsubstituted heteroalkyl.
At least one of R4, R »4- , R", R , R" , R . 112', R 1'3J, R , 1"5 or Rl b links the drug to L1, if present, or to F, H, J, or X2.
Another embodiment of the drug (D) comprises a structure (k) where R4 and R4 have been joined to from a heterocycloalkyl:
Figure imgf000125_0001
In this structure, R3, R5, R5 , R6, R7, X are as described above for Formula (e). Furthermore, Z is a member selected from O, S and NR23, wherein R23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl;
R32 is selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, halogen, NO2, NR15R16, NC(O)R15, OC(O)NR15R16, OC(O)OR15, C(O)R15, SR15, OR15, CRI5=NR16, and O(CH2)nN(CH3)2, where n is an integer from 1 to 20. R15 and R16 independently represent H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl and substituted or unsubstituted peptidyl, where R15 and R16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms. R32 optionally contains one or more cleavable groups within its structure, such as a cleavable linker or cleavable substrate. Exemplary cleavable groups include, but are not limited to, peptides, amino acids, hydrazines, disulfides, and cephalosporin derivatives. Moreover, any selection of substituents described herein for R4, R4 , R5, R5', R'\ and R16 is also applicable to R ".
At least one of R5, R5', R1 ' 'R12, R13, R15, R16, or R32 links the drug to L1, if present, or to F, H, J, or X2. In at least some embodiments, R32 links the drug to L1, if present, or to F, H, J, or X2.
One preferred embodiment of this compound is:
Figure imgf000126_0001
R is H, substituted or unsubstituted lower alkyl, C(O)R8, or CO2R , wherein R8 is a member selected from NR0R10 and OR9, in which R9 and R10 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl;
R1 is H, substituted or unsubstituted lower alkyl, or C(O)R8, wherein R8 is a member selected from NR9R10 and OR9, in which R9 and R10 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl;
R" is H, or substituted or unsubstituted lower alkyl or unsubstituted heteroalkyl or cyano or alkoxy; and R~ is H, or substituted or unsubstituted lower alkyl or unsubstituted heteroalkyl.
A further embodiment has the formula:
Figure imgf000126_0002
In this structure, A, R6, R7, X, R4, R4 , R5, and R5 are as described above for Formula (e). Furthermore, Z is a member selected from O, S and NR23, where R23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl;
R is selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, halogen, NO2, NR15R16, NC(O)R15, OC(O)NR15R16, OC(O)OR15,
C(O)R15, SR15, OR'5, CRI 5=NR16, and O(CH2)nN(CH3)2, where n is an integer from 1 to 20. R13 and R16 independently represent H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl and substituted or unsubstituted peptidyl, where R15 and R16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms. R33 links the drug to L1, if present, or to F, H, J, or X2.
Preferably, A is substituted or unsubstituted phenyl or substituted or unsubstituted pyrrole. Moreover, any selection of substituents described herein for R is also applicable to R33.
Ligands
X4 represents a ligand selected from the group consisting of protected reactive functional groups, unprotected reactive functional groups, detectable labels, and targeting agents. Preferred ligands are targeting agents, such as antibodies and fragments thereof. In some embodiments, the group X can be described as a member selected from R29, COOR29, C(O)NR29, and C(O)NNR29 wherein R29 is a member selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted heteroaryl. In yet another exemplary embodiment, R29 is a thiol reactive member. In a further exemplary embodiment, R29 is a thiol reactive member selected from haloacetyl and alkyl halide derivatives, maleimides, aziridines, and acryloyl derivatives. The above thiol reactive members can act as reactive protective groups that can be reacted with, for example, a side chain of an amino acid of a targeting agent, such as an antibody, to thereby link the targeting agent to the linker-drug moiety. Detectable Labels The particular label or detectable group used in conjunction with the compounds and methods of the invention is generally not a critical aspect of the invention, as long as it does not significantly interfere with the activity or utility of the compound of the invention. The detectable group can be any material having a detectable physical or chemical property. Such detectable labels have been well developed in the field of immunoassays and, in general, most any label useful in such methods can be applied to the present invention. Thus, a label is any composition detectable by spectroscopic, photochemical, biochemical, immunochemical, electrical, optical or chemical means. Useful labels in the present invention include magnetic beads (e.g., DYNABEADS ΓM), fluorescent dyes (e.g., fluorescein isothiocyanate, Texas red, rhodamine, and the like), radiolabels (e.g., 3H, 1251, 35S, 14C, or 32P), enzymes (e.g., horse radish peroxidase, alkaline phosphatase and others commonly used in an ELISA), and colorimetric labels such as colloidal gold or colored glass or plastic beads (e.g., polystyrene, polypropylene, latex, etc.). The label may be coupled directly or indirectly to a compound of the invention according to methods well known in the art. As indicated above, a wide variety of labels may be used, with the choice of label depending on sensitivity required, ease of conjugation with the compound, stability requirements, available instrumentation, and disposal provisions. When the compound of the invention is conjugated to a detectable label, the label is preferably a member selected from the group consisting of radioactive isotopes, fluorescent agents, fluorescent agent precursors, chromophores, enzymes and combinations thereof. Methods for conjugating various groups to antibodies are well known in the art. For example, a detectable label that is frequently conjugated to an antibody is an enzyme, such as horseradish peroxidase, alkaline phosphatase, β- galactosidase, and glucose oxidase.
Non-radioactive labels are often attached by indirect means. Generally, a ligand molecule (e.g., biotin) is covalently bound to a component of the conjugate. The ligand then binds to another molecules (e.g., streptavidin) molecule, which is either inherently detectable or covalently bound to a signal system, such as a detectable enzyme, a fluorescent compound, or a chemiluminescent compound. Components of the conjugates of the invention can also be conjugated directly to signal generating compounds, e.g., by conjugation with an enzyme or fluorophore. Enzymes of interest as labels will primarily be hydrolases, particularly phosphatases, esterases and glycosidases, or oxidotases, particularly peroxidases. Fluorescent compounds include fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbel liferone, etc. Chemiluminescent compounds include luciferin, and 2,3- dihydrophthalazinediones, e.g., luminol. For a review of various labeling or signal producing systems that may be used, see, U.S. Patent No. 4,391,904.
Means of detecting labels are well known to those of skill in the art. Thus, for example, where the label is a radioactive label, means for detection include a scintillation counter or photographic film as in autoradiography. Where the label is a fluorescent label, it may be detected by exciting the fluorochrome with the appropriate wavelength of light and detecting the resulting fluorescence. The fluorescence may be detected visually, by means of photographic film, by the use of electronic detectors such as charge coupled devices (CCDs) or photomultipliers and the like. Similarly, enzymatic labels may be detected by providing the appropriate substrates for the enzyme and detecting the resulting reaction product. Finally simple colorimetric labels may be detected simply by observing the color associated with the label. Thus, in various dipstick assays, conjugated gold often appears pink, while various conjugated beads appear the color of the bead.
Fluorescent labels are presently preferred as they have the advantage of requiring few precautions in handling, and being amenable to high-throughput visualization techniques (optical analysis including digitization of the image for analysis in an integrated system comprising a computer). Preferred labels are typically characterized by one or more of the following: high sensitivity, high stability, low background, low environmental sensitivity and high specificity in labeling. Many fluorescent labels are commercially available from the SIGMA chemical company (Saint Louis, MO), Molecular Probes (Eugene, OR), R&D systems (Minneapolis, MN), Pharmacia LKB Biotechnology (Piscataway, NJ), CLONTECH Laboratories, Inc. (Palo Alto, CA), Chem Genes Corp., Aldrich Chemical Company (Milwaukee, Wl), Glen Research, Inc., GIBCO BRL Life Technologies, Inc. (Gaithersburg, MD), Fluka Chemica- Biochemika Analytika (Fluka Chemie AG, Buchs, Switzerland), and Applied Biosystems (Foster City, CA), as well as many other commercial sources known to one of skill. Furthermore, those of skill in the art will recognize how to select an appropriate fluorophore for a particular application and, if it is not readily available commercially, will be able to synthesize the necessary fluorophore de novo or synthetically modify commercially available fluorescent compounds to arrive at the desired fluorescent label.
In addition to small molecule fluorophores, naturally occurring fluorescent proteins and engineered analogues of such proteins are useful in the present invention. Such proteins include, for example, green fluorescent proteins of cnidarians (Ward et ai, Photochem. Photobiol. 35:803-808 (1982); Levine et al, Comp. Biochem. Physiol,
72B:77-85 (1982)), yellow fluorescent protein from Vibrio fischeri strain (Baldwin et ai, Biochemistry 29:5509-15 (1990)), Peridinin-chlorophyll from the dinoflagellate Symbiodinium sp. (Morris et ai, Plant Molecular Biology 24:673:77 (1994)), phycobiliproteins from marine cyanobacteria, such as Synechococcus, e.g., phycoerythrin and phycocyanin (Wilbanks et al, J. Biol. Chem. 268: 1226-35 ( 1993)), and the like.
Generally, prior to forming the linkage between the cytotoxin and the targeting (or other) agent, and optionally, the spacer group, at least one of the chemical functionalities will be activated. One skilled in the art will appreciate that a variety of chemical functionalities, including hydroxy, amino, and carboxy groups, can be activated using a variety of standard methods and conditions. For example, a hydroxyl group of the cytotoxin or targeting agent can be activated through treatment with phosgene to form the corresponding chloroformate, or p-nitrophenylchloroformate to form the corresponding carbonate.
In an exemplary embodiment, the invention makes use of a targeting agent that includes a carboxyl functionality. Carboxyl groups may be activated by, for example, conversion to the corresponding acyl halide or active ester. This reaction may be performed under a variety of conditions as illustrated in March, supra pp. 388-89. In an exemplary embodiment, the acyl halide is prepared through the reaction of the carboxyl- containing group with oxalyl chloride. The activated agent is reacted with a cytotoxin or cytotoxin-linker arm combination to form a conjugate of the invention. Those of skill in the art will appreciate that the use of carboxyl-containing targeting agents is merely illustrative, and that agents having many other functional groups can be conjugated to the linkers of the invention.
Reactive Functional Groups
For clarity of illustration the succeeding discussion focuses on the conjugation of a cytotoxin to a targeting agent. The focus exemplifies one embodiment of the invention from which, others are readily inferred by one of skill in the art. No limitation of the invention is implied, by focusing the discussion on a single embodiment.
Exemplary compounds of the invention bear a reactive functional group, which is generally located on a substituted or unsubstituted alkyj.or heteroalkyl chain, allowing their facile attachment to another species. A convenient location for the reactive group is the terminal position of the chain.
Reactive groups and classes of reactions useful in practicing the present invention are generally those that are well known in the art of bioconjugate chemistry. The reactive functional group may be protected or unprotected, and the protected nature of the group may be changed by methods known in the art of organic synthesis. Preferred classes of reactions available with reactive cytotoxin analogues are those which proceed under relatively mild conditions. These include, but are not limited to nucleophilic substitutions (e.g., reactions of amines and alcohols with acyl halides, active esters), electrophilic substitutions (e.g., enamine reactions) and additions to carbon-carbon and carbon- heteroatom multiple bonds (e.g., Michael reaction, Diels-Alder addition). These and other useful reactions are discussed in, for example, March, Advanced Organic Chemistry, 3rd Ed., John Wiley & Sons, New York, 1985; Hermanson, Bioconjugate Techniques, Academic Press, San Diego, 1996; and Feeney et ai, Modification of Proteins; Advances in Chemistry Series, Vol. 198, American Chemical Society, Washington, D.C., 1982.
Exemplary reaction types include the reaction of carboxyl groups and various derivatives thereof including, but not limited to, N-hydroxysuccinimide esters, N- hydroxybenztriazole esters, acid halides, acyl imidazoles, thioesters, p-nitrophenyl esters, alkyl, alkenyl, alkynyl and aromatic esters. Hydroxyl groups can be converted to esters, ethers, aldehydes, etc. Haloalkyl groups are converted to new species by reaction with, for example, an amine, a carboxylate anion, thiol anion, carbanion, or an alkoxide ion. Dienophile (e.g., maleimide) groups participate in Diels-Alder. Aldehyde or ketone groups can be converted to imines, hydrazones, semicarbazones or oximes, or via such mechanisms as Grignard addition or alkyllithium addition. Sulfonyl halides react readily with amines, for example, to form sulfonamides. Amine or sulfhydryl groups are, for example, acylated, alkylated or oxidized. Alkenes, can be converted to an array of new species using cycloadditions, acylation, Michael addition, etc. Epoxides react readily with amines and hydroxyl compounds.
One skilled in the art will readily appreciate that many of these linkages may be produced in a variety of ways and using a variety of conditions. For the preparation of esters, see, e.g., March supra at 1157; for thioesters, see, March, supra at 362-363, 491, 720-722, 829, 941, and 1 172; for carbonates, see, March, supra at 346-347; for carbamates, see, March, supra at 1 156-57; for amides, see, March supra at 1152; for ureas and thioureas, see, March supra at 1174; for acetals and ketals, see, Greene et al. supra 178-210 and March supra at 1146; for acyloxyalkyl derivatives, see, Prodrugs: Topical and Ocular Drug Delivery, K. B. Sloan, ed., Marcel Dekker, Inc., New York, 1992; for enol esters, see, March supra at 1 160; for N-sulfonylimidates, see, Bundgaard et al., J. Med. Chem., 31:2066 (1988); for anhydrides, see, March s«/>ra at 355-56, 636- 37, 990-91 , and 1 154; for N-acylamides, see, March supra at 379; for N-Mannich bases, see, March supra at 800-02, and 828; for hydroxymethyl ketone esters, see, Petracek et al. Annals NY Acad. ScL, 507:353-54 (1987); for disulfides, see, March supra at 1 160; and for phosphonate esters and phosphonamidates.
The reactive functional groups can be unprotected and chosen such that they do not participate in, or interfere with, the reactions. Alternatively, a reactive functional group can be protected from participating in the reaction by the presence of a protecting group. Those of skill in the art will understand how to protect a particular functional group from interfering with a chosen set of reaction conditions. For examples of useful protecting groups, See Greene et al., Protective Groups in Organic Synthesis, John Wiley & Sons, New York, 1991.
Typically, the targeting agent is linked covalently to a cytotoxin using standard chemical techniques through their respective chemical functionalities. Optionally, the linker or agent is coupled to the agent through one or more spacer groups. The spacer groups can be equivalent or different when used in combination.
Generally, prior to forming the linkage between the cytotoxin and the reactive functional group, and optionally, the spacer group, at least one of the chemical functionalities will be activated. One skilled in the art will appreciate that a variety of chemical functionalities, including hydroxy, amino, and carboxy groups, can be activated using a variety of standard methods and conditions. In an exemplary embodiment, the invention comprises a carboxyl functionality as a reactive functional group. Carboxyl groups may be activated as described hereinabove. Cleavable Substrate
The cleavable substrates of the current invention are depicted as "X2". Preferably, the cleavable substrate is a cleavable enzyme substrate that can be cleaved by an enzyme. Preferably, the enzyme is preferentially associated, directly or indirectly, with the tumor or other target cells to be treated. The enzyme may be generated by the tumor or other target cells to be treated. For example, the cleavable substrate can be a peptide that is preferentially cleavable by an enzyme found around or in a tumor or other target cell. Additionally or alternatively, the enzyme can be attached to a targeting agent that binds specifically to tumor cells, such as an antibody specific for a tumor antigen.
As examples of enzyme cleavable substrates suitable for coupling to the drugs described above, PCT Patent Applications Publication Nos. WO 00/33888, WO 01/95943, WO 01/95945, WO 02/00263, and WO 02/100353, all of which are incorporated herein by reference, disclose attachment of a cleavable peptide to a drug. The peptide is cleavable by an enzyme, such as a trouase (such as thimet oligopeptidase), CDlO (neprilysin), a matrix metal lopro tease (such as MMP2 or MMP9), a type II transmembrane serine protease (such as Hepsin, testisin, TMPRSS4, or matriptase/MT- SPl), or a cathepsin, associated with a tumor. In this embodiment, a prodrug includes the drug as described above, a peptide, a stabilizing group, and optionally a linking group between the drug and the peptide. The stabilizing group is attached to the end of the peptide to protect the prodrug from degradation before arriving at the tumor or other target cell. Examples of suitable stabilizing groups include non-amino acids, such as succinic acid, diglycolic acid, maleic acid, polyethylene glycol, pyroglutamic acid, acetic acid, naphthylcarboxylic acid, terephthalic acid, and glutaric acid derivatives; as well as non-genetically-coded amino acids or aspartic acid or glutamic acid attached to the N- terminus of the peptide at the β-carboxy group of aspartic acid or the γ-carboxyl group of glutamic acid. The peptide typically includes 3-12 (or more) amino acids. The selection of particular amino acids will depend, at least in part, on the enzyme to be used for cleaving the peptide, as well as, the stability of the peptide in vivo. One example of a suitable cleavable peptide is β-AlaLeuAlaLeu (SEQ ID NO: 102). This can be combined with a stabilizing group to form succinyl-β-AlaLeuAlaLeu (SEQ ID NO: 102). Other examples of suitable cleavable peptides are provided in the references cited above.
As one illustrative example, CDlO, also known as neprilysin, neutral endopeptidase (NEP), and common acute lymphoblastic leukemia antigen (CALLA), is a type II cell-surface zinc-dependent metalloprotease. Cleavable substrates suitable for use with CDlO include LeuAlaLeu and IleAlaLeu. Other known substrates for CDlO include peptides of up to 50 amino acids in length, although catalytic efficiency often declines as the substrate gets larger.
Another illustrative example is based on matrix metalloproteases (MMP). Probably the best characterized proteolytic enzymes associated with tumors, there is a clear correlation of activation of MMPs within tumor microenvironments. In particular, the soluble matrix enzymes MMP2 (gelatinase A) and MMP9 (gelatinase B), have been intensively studied, and shown to be selectively activated during tissue remodeling including tumor growth. Peptide sequences designed to be cleaved by MMP2 and MMP9 have been designed and tested for conjugates of dextran and methotrexate (Chau et al, Bioconjugate Chem. 15:931-941 (2004)); PEG (polyethylene glycol) and doxorubicin (Bae et al. , Drugs Exp. Clin. Res. 29: 15-23 (2004)); and albumin and doxorubicin (Kratz et al., Bioorg. Med. Chem. Lett. 1 1:2001-2006 (2001)). Examples of suitable sequences for use with MMPs include, but are not limited to, ProValGlyLeuIleGly (SEQ ID NO: 95), GlyProLeuGlyVal (SEQ ID NO:96), GlyProLeuGlylleAlaGlyGln (SEQ ID NO: 97), ProLeuGlyLeu (SEQ ID NO: 98), GlyProLeuGlyMetLeuSerGln (SEQ ID NO: 99), and GlyProLeuGlyLeuTrpAlaGln (SEQ ID NO: 100). (See, e.g., the previously cited references as well as Kline et a /., MoI. Pharmaceut. 1 :9-22 (2004) and Liu et a /., Cancer Res. 60:6061-6067 (2000).) Other cleavable substrates can also be used.
Yet another example is type II transmembrane serine proteases. This group of enzymes includes, for example, hepsin, testisin, and TMPRSS4. GlnAlaArg is one substrate sequence that is useful with matriptase/MT-SPl (which is over-expressed in breast and ovarian cancers) and LeuSerArg is useful with hepsin (over-expressed in prostate and some other tumor types). (See, e.g., Lee et. ai, J. Biol. Chem. 275:36720- 36725 and Kurachi and Yamamoto, Handbook of Proeolytic Enzymes Vol. 2, 2nd edition (Barrett AJ, Rawlings ND & Woessner JF, eds) pp. 1699- 1702 (2004).) Other cleavable substrates can also be used.
Another type of cleavable substrate arrangement includes preparing a separate enzyme capable of cleaving the cleavable substrate that becomes associated with the tumor or cells. For example, an enzyme can be coupled to a tumor-specific antibody (or other entity that is preferentially attracted to the tumor or other target cell such as a receptor ligand) and then the enzyme-antibody conjugate can be provided to the patient. The enzyme-antibody conjugate is directed to, and binds to, antigen associated with the tumor. Subsequently, the drug-cleavable substrate conjugate is provided to the patient as a prodrug. The drug is only released in the vicinity of the tumor when the drug-cleavable substrate conjugate interacts with the enzyme that has become associated with the tumor so that the cleavable substrate is cleaved and the drug is freed. For example, U.S. Patents Nos. 4,975,278; 5,587,161; 5,660,829; 5,773,435; and 6,132,722, all of which are incorporated herein by reference, disclose such an arrangement. Examples of suitable enzymes and substrates include, but are not limited to, β-lactamase and cephalosporin derivatives, carboxypeptidase G2 and glutamic and aspartic folate derivatives. In one embodiment, the enzyme-antibody conjugate includes an antibody, or antibody fragment, that is selected based on its specificity for an antigen expressed on a target cell, or at a target site, of interest. A discussion of antibodies is provided hereinabove. One example of a suitable cephalosporin-cleavable substrate is
Figure imgf000136_0001
Examples Of Conjugates
The linkers and cleavable substrates of the invention can be used in conjugates containing a variety of partner molecules. Examples of conjugates of the invention are described in further detail below. Unless otherwise indicated, substituents are defined as set forth above in the sections regarding cytotoxins, linkers, and cleavable substrates.
A. Linker Conjugates One example of a suitable conjugate is a compound of the formula:
X" -(L4L-F-(L 1).-^D
wherein L1 is a self-immolative linker; m is an integer 0, 1, 2, 3, 4, 5, or 6; F is a linker comprising the structure:
Figure imgf000136_0002
wherein AA1 is one or more members independently selected from the group consisting of natural amino acids and unnatural α- amino acids; c is an integer from 1 to 20; L2 is a self-immolative linker and comprises
Figure imgf000137_0001
wherein each R . 17 , R , 18 , and R , 19 i •s independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryi, and w is an integer from 0 to 4; o is 1; L4 is a linker member; p is 0 or 1; X4 is a member selected from the group consisting of protected reactive functional groups, unprotected reactive functional groups, detectable labels, and targeting agents; and D comprises a structure:
Figure imgf000137_0002
wherein the ring system A is a member selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups; E and G are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, a heteroatom, a single bond, or E and G are joined to form a ring system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl; X is a member selected from O, S and NR23; R23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl; RJ is OR1 ', wherein R1 1 is a member selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, monophosphates, diphosphates, triphosphates, sulfonates, acyl, C(O)R12R13, C(O)OR12, C(O)NR12R13, P(O)(OR1O2, , C(O)CHR i I'2~Rπ 13 , SR , 1U2 and
Figure imgf000137_0003
R4 , R ,4- , RΛ and R" are members independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO2, NR13R16, NC(O)R15, OC(O)NR15R16, OC(O)OR15, C(O)R15, SR15, OR15, CR15=NR16, and O(CH2)nN(CH3)2, or any adjacent pair of R4, R4', R5 and R5", together with the carbon atoms to which they are attached, are joined to form a substituted or unsubstituted cycloalkyl or heterocycloalkyl ring system having from 4 to 6 members; wherein n is an integer from 1 to 20; R15 and R16 are independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, and substituted or unsubstituted peptidyl, wherein R15 and R16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms; R6 is a single bond which is either present or absent and when present R6 and R7 are joined to form a cyclopropyl ring; and R7 is CH2-X1 Or -CH2- joined in said cyclopropyl ring with R6, wherein X1 is a leaving group, wherein R11 links said drug to L1, if present, or to F.
In some embodiments, the drug has structure (c) or (f) above. One specific example of a compound suitable for use as a conjugate is
Figure imgf000138_0001
Another example of a type of conjugate is a compound of the formula
Figure imgf000138_0002
wherein L1 is a self-immolative linker; m is an integer 0, 1, 2, 3, 4, 5, or 6; F is a linker comprising the structure:
Figure imgf000139_0001
wherein AA is one or more members independently selected from the group consisting of natural amino acids and unnatural α-amino acids; c is an integer from 1 to 20; L2 is a self-immolative linker; o is 0 or 1 ; L4 is a linker member; p is 0 or 1 ; X4 is a member selected from the group consisting of protected reactive functional groups, unprotected reactive functional groups, detectable labels, and targeting agents; and D comprises a structure:
Figure imgf000139_0002
wherein the ring system A is a member selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups; E and G are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, a heteroatom, a single bond, or E and G are joined to form a ring system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl; X is a member selected from O, S and NR23; R23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl; R"1 is a member selected from the group consisting of (=0), SR11, NHR11 and OR1 1, wherein R11 is a member selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, monophosphates, diphosphates, triphosphates, sulfonates, acyl, C(O)R12R13, C(O)OR12, C(O)NR12R13, P(O)(ORI2)2, , C(O)CHR12R13, SR12 and SiR12R13R14, in which R12, R13, and R14 are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryl, wherein R12 and R13 together with the nitrogen or carbon atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms; R4 , R4', Rs and R5' are members independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO2, NR15R16, NC(O)R15, OC(O)NR15R16, OC(O)OR15, C(O)R15, SR15, OR15, CR15=NR16, and O(CH2)nN(CH3)2, or any adjacent pair of R4, R4', R5 and R5', together with the carbon atoms to which they are attached, are joined to form a substituted or unsubstituted cycloalkyl or heterocycloalkyl ring system having from 4 to 6 members, wherein n is an integer from 1 to 20; R15 and R16 are independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, and substituted or unsubstituted peptidyl, wherein R15 and R16 together with the nitrogen atom to which they are attached are optionally joined to foπn a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms; wherein at least one of R , R ', R" and R5' links said drug to L1, if present, or to F, and comprises
Figure imgf000140_0001
wherein v is an integer from 1 to 6; and each R27, R27 , R2S, and R28 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl; R6 is a single bond which is either present or absent and when present R and R are joined to form a cyclopropyl ring; and R is CH2-X1 or -CH2- joined in said cyclopropyl ring with R6, wherein X1 is a leaving group.
In some embodiment, the drug has structure (c) or (f) above. One specific example of a compound suitable for use as a conjugate is
Figure imgf000141_0001
where r is an integer in the range from 0 to 24.
Another example of a suitable conjugate is a compound of the formula
"(L4)p-F- -(L1) TD wherein L1 is a self-immolative linker; m is an integer 0, 1, 2, 3, 4, 5, or 6; F is a linker comprising the structure:
Figure imgf000141_0002
wherein AA is one or more members independently selected from the group consisting of natural amino acids and unnatural α-amino acids; c is an integer from 1 to 20; L3 is a spacer group comprising a primary or secondary amine or a carboxyl functional group; wherein if LJ is present, m is 0 and either the amine of L' forms an amide bond with a pendant carboxyl functional group of D or the carboxyl of LJ forms an amide bond with a pendant amine functional group of D; o is 0 or 1; L4 is a linker member, wherein L4 comprises
Figure imgf000141_0003
directly attached to the N-terminus of (AA )c, wherein R >20 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl, each R25, R25 , R26, and R26 is independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl; and s and t are independently integers from 1 to 6; p is 1; X4 is a member selected from the group consisting of protected reactive functional groups, unprotected reactive functional groups, detectable labels, and targeting agents; and D comprises a structure:
Figure imgf000142_0001
wherein the ring system A is a member selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups; E and G are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, a heteroatom, a single bond, or E and G are joined to form a ring system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl; X is a member selected from O, S and NR23; R23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl; R' is a member selected from the group consisting of (=O), SR1 ', NHR1 ' and OR1 ', wherein R1 1 is a member selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, monophosphates, diphosphates, triphosphates, sulfonates, acyl, C(O)R12R13, C(O)OR12, C(O)NR12R13, P(O)(OR12)2, , C(O)CHR12R13, SR12 and SiR12R13R14, in which R12, R13, and R14 are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryl, wherein R12 and R13 together with the nitrogen or carbon atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms; R4 , R4', R5 and R5' are members independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO2, NR15R16, NC(O)R15, OC(O)NR15R16, OC(O)OR15, C(O)R15, SR15, OR15, CRI5=NR16, and O(CH2)nN(CH3)2, or any adjacent pair of R4, R4', R5 and R5\ together with the carbon atoms to which they are attached, are joined to form a substituted or unsubstituted cycloalkyl or heterocycloalkyl ring system having from 4 to 6 members, wherein n is an integer from 1 to 20; R13 and R16 are independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, and substituted or unsubstituted peptidyl, wherein R15 and R16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms; R6 is a single bond which is either present or absent and when present R6 and R7 are joined to form a cyclopropyl ring; and R7 is CH2-X1 Or -CH2- joined in said cyclopropyl ring with R6, wherein X1 is a leaving group, wherein at least one of R4 , R4', R5, R""", R1 s or R16 links said drug to L1, if present, or to F.
In some embodiments, the drug has structure (c) or (f) above. One specific example of a compound suitable for use as conjugate is
Figure imgf000143_0001
where r is an integer in the range from 0 to 24.
Other examples of suitable compounds for use as conjugates include:
Figure imgf000143_0002
Figure imgf000144_0001
Figure imgf000144_0002
and
Figure imgf000144_0003
Figure imgf000145_0001
Figure imgf000146_0001
Figure imgf000147_0001
Figure imgf000147_0002
Figure imgf000147_0003
where R is
Figure imgf000147_0004
oorr and r is an integer in the range from 0 to 24.
Conjugates can also be formed using the drugs having structure (g), such as the following compounds:
Figure imgf000148_0001
Figure imgf000148_0002
Figure imgf000148_0003
Figure imgf000148_0004
Figure imgf000149_0001
Figure imgf000149_0002
Figure imgf000149_0003
(where r is an integer in the range from 0 to 24.
Conjugates can also be foπned using the drugs having the following structures:
Figure imgf000150_0001
Figure imgf000150_0002
Figure imgf000150_0003
Figure imgf000151_0001
Figure imgf000151_0002
Figure imgf000152_0001
and
Figure imgf000153_0001
Synthesis of such cytotoxins, as well as details regarding their linkage to antibodies is disclosed in U.S. Patent Application Serial No. 60/991 ,300, filed on November 30, 2007. In certain embodiments, the anti-CD 19 is conjugated to the linker and therapeutic agent of structure N 1 :
O //
~~O
~"N
Figure imgf000153_0002
N - Nl (Anti-CDl 1-))
In certain embodiments, the anti-CD 19 is conjugated to the linker and therapeutic agent of structure N2:
Figure imgf000154_0001
O
N2
B. Cleavable Linker Conjugates
One example of a suitable conjugate is a compound having the following structure:
Figure imgf000154_0002
wherein L1 is a self-immolative spacer; m is an integer of 0, 1, 2, 3, 4, 5, or 6; X2 is a cleavable substrate; and D comprises a structure:
Figure imgf000154_0003
wherein the ring system A is a member selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl groups; E and G are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, a heteroatom, a single bond, or E and G are joined to form a πng system selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl; X is a member selected from O, S and NR2'; R2' is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, and acyl; R3 is a member selected from the group consisting of (=0), SR1 1. NHR11 and OR1 1, wherein R1 1 is a member selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted heteroalkyl, unsubstituted heteroalkyl, diphosphates, triphosphates, acyl, C(O)R12R13, C(O)OR12, C(O)NR12R13. P(O)(OR12)2, , C(O)CHR12R13, SR12 and SiR12R13R14, in which R12, R13, and R14 are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted aryl, wherein R12 and R13 together with the nitrogen or carbon atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms; R6 is a single bond which is either present or absent and when present R6 and R7 are joined to foπn a cyclopropyl ring; and R7 is CH2-X1 or -CH2- joined in said cyclopropyl ring with R6, wherein X1 is a leaving group, R4 , R4', R5 and R5' are members independently selected from the group consisting of H, substituted alkyl, unsubstituted alkyl, substituted aryl, unsubstituted aryl, substituted heteroaryl, unsubstituted heteroaryl, substituted heterocycloalkyl, unsubstituted heterocycloalkyl, halogen, NO2, NR15R16, NC(O)R15, OC(O)NR15R16, OC(O)OR15. C(O)R15, SR15, OR15, CRI 5=NR16, and O(CH2)nN(CH3)2, or any adjacent pair of R4, R4', R5 and R5', together with the carbon atoms to which they are attached, are joined to foπn a substituted or unsubstituted cycloalkyl or heterocycloalkyl ring system having from 4 to 6 members, wherein n is an integer from 1 to 20; R15 and R16 are independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, and substituted or unsubstituted peptidyl, wherein R15 and R16 together with the nitrogen atom to which they are attached are optionally joined to form a substituted or unsubstituted heterocycloalkyl ring system having from 4 to 6 members, optionally containing two or more heteroatoms; wherein at least one of members R4 , R4', Rs and R5' links said drug to L1, if present, or to X2, and is selected from the group consisting of
Figure imgf000156_0001
wherein R30, R30 , R3', and R31 are independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl; and v is an integer from 1 to 6.
Examples of suitable cleavable linkers include β-AlaLeuAlaLeu (SEQ ID NO: 102) and
Figure imgf000156_0002
Pharmaceutical Compositions
In another aspect, the present disclosure provides a composition, e.g., a pharmaceutical composition, containing one or a combination of monoclonal antibodies, or antigen-binding portion(s) thereof, of the present disclosure, formulated together with a pharmaceutically acceptable carrier. Such compositions may include one or a combination of {e.g., two or more different) antibodies, or immunoconjugates or bispecific molecules of this disclosure. For example, a pharmaceutical composition of this disclosure can comprise a combination of antibodies (or immunoconjugates or bispecifics) that bind to different epitopes on the target antigen or that have complementary activities. Pharmaceutical compositions of this disclosure also can be administered in combination therapy, i.e., combined with other agents. For example, the combination therapy can include an anti-CD 19 antibody of the present disclosure combined with at least one other anti-cancer agent. Examples of therapeutic agents that can be used in combination therapy are described in greater detail below in the section on uses of the antibodies of this disclosure.
As used herein, "pharmaceutically acceptable earner" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible. Preferably, the carrier is suitable for intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion). Depending on the route of administration, the active compound, i.e., antibody, immunoconjugate, or bispecific molecule, may be coated in a material to protect the compound from the action of acids and other natural conditions that may inactivate the compound. The pharmaceutical compounds of this disclosure may include one or more pharmaceutically acceptable salts. A "pharmaceutically acceptable salt" refers to a salt that retains the desired biological activity of the parent compound and does not impart any undesired toxicological effects (see e.g.. Berge, S. M., et al. (1977) J. Pharm. Sci. 66: 1-19). Examples of such salts include acid addition salts and base addition salts. Acid addition salts include those derived from nontoxic inorganic acids, such as hydrochloric, nitric, phosphoric, sulfuric, hydrobromic, hydroiodic, phosphorous and the like, as well as from nontoxic organic acids such as aliphatic mono- and dicarboxylic acids, phenyl- substituted alkanoic acids, hydroxy alkanoic acids, aromatic acids, aliphatic and aromatic sulfonic acids and the like. Base addition salts include those derived from alkaline earth metals, such as sodium, potassium, magnesium, calcium and the like, as well as from nontoxic organic amines, such as N.N'-dibenzylethylenediamine, N-methylglucamine, chloroprocaine, choline, diethanolamine, ethylenediamine, procaine and the like.
A pharmaceutical composition of this disclosure also may include a pharmaceutically acceptable anti-oxidant. Examples of pharmaceutically acceptable antioxidants include: (1) water soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfite, sodium sulfite and the like; (2) oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA)5 butylated hydroxytoluene (BHT), lecithin, propyl gallate, alpha-tocopherol, and the like; and (3) metal chelating agents, such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like. Examples of suitable aqueous and nonaqueous carriers that may be employed in the pharmaceutical compositions of this disclosure include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), and suitable mixtures thereof, vegetable oils, such as olive oil, and injectable organic esters, such as ethyl oleate. Proper fluidity can be maintained, for example, by the use of coating materials, such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.
These compositions may also contain adjuvants such as preservatives, wetting agents, emulsifying agents and dispersing agents. Prevention of presence of microorganisms may be ensured both by sterilization procedures, supra, and by the inclusion of various antibacterial and antifungal agents, for example, paraben, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents which delay absorption such as aluminum monostearate and gelatin.
Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. The use of such media and agents for pharmaceutically active substances is known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the pharmaceutical compositions of this disclosure is contemplated. Supplementary active compounds can also be incorporated into the compositions.
Therapeutic compositions typically must be sterile and stable under the conditions of manufacture and storage. The composition can be formulated as a solution, microemulsion, liposome, or other ordered structure suitable to high drug concentration. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, or sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent that delays absorption, for example, monostearate salts and gelatin.
Sterile injectable solutions can be prepared by incoiporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by sterilization microfiltration. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying (lyophilization) that yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
The amount of active ingredient which can be combined with a carrier material to produce a single dosage form will vary depending upon the subject being treated, and the particular mode of administration. The amount of active ingredient which can be combined with a earner material to produce a single dosage form will generally be that amount of the composition which produces a therapeutic effect. Generally, out of one hundred per cent, this amount will range from about 0.01 per cent to about ninety-nine percent of active ingredient, preferably from about 0.1 per cent to about 70 per cent, most preferably from about 1 per cent to about 30 per cent of active ingredient in combination with a pharmaceutically acceptable carrier.
Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response). For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of this disclosure are dictated by and directly dependent on (a) the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and (b) the limitations inherent in the art of compounding such an active compound for the treatment of sensitivity in individuals. For administration of the antibody, the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to 5 mg/kg, of the host body weight. For example dosages can be 0.3 mg/kg body weight, 1 mg/kg body weight, 3 mg/kg body weight, 5 mg/kg body weight or 10 mg/kg body weight or within the range of 1-10 mg/kg. An exemplary treatment regime entails administration once per week, once every two weeks, once every three weeks, once every four weeks, once a month, once every 3 months or once every three to 6 months. Preferred dosage regimens for an anti-CD 19 antibody of this disclosure include 1 mg/kg body weight or 3 mg/kg body weight via intravenous administration, with the antibody being given using one of the following dosing schedules: (i) every four weeks for six dosages, then every three months; (ii) every three weeks; (iii) 3 mg/kg body weight once followed by 1 mg/kg body weight every three weeks.
In some methods, two or more monoclonal antibodies with different binding specificities are administered simultaneously, in which case the dosage of each antibody administered falls within the ranges indicated. Antibody is usually administered on multiple occasions. Intervals between single dosages can be, for example, weekly, monthly, every three months or yearly. Intervals can also be irregular as indicated by measuring blood levels of antibody to the target antigen in the patient. In some methods, dosage is adjusted to achieve a plasma antibody concentration of about 1-1000 μg /ml and in some methods about 25-300 μg /ml.
Alternatively, antibody can be administered as a sustained release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the antibody in the patient. In general, human antibodies show the longest half life, followed by humanized antibodies, chimeric antibodies, and nonhuman antibodies. The dosage and frequency of administration can vary depending on whether the treatment is prophylactic or therapeutic. In prophylactic applications, a relatively low dosage is administered at relatively infrequent intervals over a long period of time. Some patients continue to receive treatment for the rest of their lives. In therapeutic applications, a relatively high dosage at relatively short intervals is sometimes required until progression of the disease is reduced or terminated, and preferably until the patient shows partial or complete amelioration of symptoms of disease. Thereafter, the patient can be administered a prophylactic regime. For use in the prophylaxis and/or treatment of diseases related to abnormal cellular proliferation, a circulating concentration of administered compound of about 0.001 μM to 20 μM is preferred, with about 0.01 μM to 5 μM being preferred.
Patient doses for oral administration of the compounds described herein, typically range from about 1 mg/day to about 10,000 mg/day, more typically from about 10 mg/day to about 1,000 mg/day, and most typically from about 50 mg/day to about 500 mg/day. Stated in terms of patient body weight, typical dosages range from about 0.01 to about 150 mg/kg/day, more typically from about 0.1 to about 15 mg/kg/day, and most typically from about 1 to about 10 mg/kg/day, for example 5 mg/kg/day or 3 mg/kg/day. In at least some embodiments, patient doses that retard or inhibit tumor growth can be 1 μmol/kg/day or less. For example, the patient doses can be 0.9, 0.8, 0.7, 0.6, 0.5, 0.45, 0.3, 0.2, 0.15, 0.1, 0.09, 0.08, 0.07, 0.06, 0.05, 0.04, 0.03, 0.02, 0.01, or 0.005 μmol/kg or less (referring to moles of the drag). Preferably, the antibody-drug conjugate retards growth of the tumor when administered in the daily dosage amount over a period of at least five days. In at least some embodiments, the tumor is a human-type tumor in a SCID mouse. As an example, the SCID mouse can be a CB 17.SCID mouse (available from Taconic, Geπnantown, NY).
Actual dosage levels of the active ingredients in the pharmaceutical compositions of the present disclosure may be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient. The selected dosage level will depend upon a variety of pharmacokinetic factors including the activity of the particular compositions of the present disclosure employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the medical arts.
A "therapeutically effective dosage" of an anti-CD 19 antibody of this disclosure preferably results in a decrease in severity of disease symptoms, an increase in frequency and duration of disease symptom-free periods, or a prevention of impairment or disability due to the disease affliction. For example, for the treatment of CD19+ tumors, a
"therapeutically effective dosage" preferably inhibits cell growth or tumor growth by at least about 20%, more preferably by at least about 40%, even more preferably by at least about 60%, and still more preferably by at least about 80% relative to untreated subjects. The ability of a compound to inhibit tumor growth can be evaluated in an animal model system predictive of efficacy in human tumors. Alternatively, this property of a composition can be evaluated by examining the ability of the compound to inhibit cell growth, such inhibition can be measured in vitro by assays known to the skilled practitioner. A therapeutically effective amount of a therapeutic compound can decrease tumor size, or otherwise ameliorate symptoms in a subject. One of ordinary skill in the art would be able to determine such amounts based on such factors as the subject's size, the severity of the subject's symptoms, and the particular composition or route of administration selected.
A composition of the present disclosure can be administered via one or more routes of administration using one or more of a variety of methods known in the art. As will be appreciated by the skilled artisan, the route and/or mode of administration will vary depending upon the desired results. Preferred routes of administration for antibodies of this disclosure include intravenous, intramuscular, intradermal, intraperitoneal, subcutaneous, spinal or other parenteral routes of administration, for example by injection or infusion. The phrase "parenteral administration" as used herein means modes of administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, epidural and intrasternal injection and infusion.
Alternatively, an antibody of this disclosure can be administered via a non- parenteral route, such as a topical, epidermal or mucosal route of administration, for example, intranasally, orally, vaginally, rectally, sublingually or topically.
The active compounds can be prepared with carriers that will protect the compound against rapid release, such as a controlled release formulation, including implants, transdeπnal patches, and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods for the preparation of such formulations are patented or generally known to those skilled in the art. See, e.g., Sustained and Controlled Release Drug Delivery Systems, J. R. Robinson, edL, Marcel Dekker, Inc., New York, 1978. Therapeutic compositions can be administered with medical devices known in the art. For example, in a preferred embodiment, a therapeutic composition of this disclosure can be administered with a needleless hypodermic injection device, such as the devices disclosed in U.S. Patent Nos. 5,399,163; 5,383,851; 5,312,335; 5,064,413; 4,941,880; 4,790,824; or 4,596,556. Examples of well-known implants and modules useful in the present disclosure include: U.S. Patent No. 4,487,603, which discloses an implantable micro-infusion pump for dispensing medication at a controlled rate; U.S. Patent No. 4,486, 194, which discloses a therapeutic device for administering medicants through the skin; U.S. Patent No. 4,447,233, which discloses a medication infusion pump for delivering medication at a precise infusion rate; U.S. Patent No. 4,447,224, which discloses a variable flow implantable infusion apparatus for continuous drug delivery; U.S. Patent No. 4,439,196, which discloses an osmotic drug delivery system having multi-chamber compartments; and U.S. Patent No. 4,475,196, which discloses an osmotic drug delivery system. These patents are incorporated herein by reference. Many other such implants, delivery systems, and modules are known to those skilled in the art. In certain embodiments, the human monoclonal antibodies of this disclosure can be formulated to ensure proper distribution in vivo. For example, the blood-brain barrier (BBB) excludes many highly hydrophilic compounds. To ensure that the therapeutic compounds of this disclosure cross the BBB (if desired), they can be formulated, for example, in liposomes. For methods of manufacturing liposomes, see, e.g., U.S. Patents 4,522,81 1 ; 5,374,548; and 5,399,331. The liposomes may comprise one or more moieties which are selectively transported into specific cells or organs, thus enhance targeted drug delivery (see, e.g., V. V. Ranade (1989) J. Clin. Pharmacol. 29:685). Exemplary targeting moieties include folate or biotin (see, e.g., U.S. Patent 5,416,016 to Low et al.); marmosides (Umezawa et ai, (1988) Biochem. Biophys. Res. Comrnun. 153:1038); antibodies (P.G. Bloeman et al. (1995) FEBS Lett. 357: 140; M. Owais et al (1995) Antimicrob. Agents Chemother. 39: 180); surfactant protein A receptor (Briscoe et al.
(1995) Am. J. Physiol. 1233:134); pi 20 (Schreier et al. (1994) J. Biol. Chem. 269:9090); see also K. Keinanen; M.L. Laukkanen (1994) FEBS Lett. 346: 123; JJ. Killion; IJ. Fidler (1994) lmmunomethods 4:273.
Uses and Methods of the Invention The antibodies, particularly the human antibodies, antibody compositions antibody-partner molecule conjugate compositions and methods of the present disclosure have numerous in vitro and in vivo diagnostic and therapeutic utilities involving the diagnosis and treatment of CD 19 mediated disorders. For example, these molecules can be administered to cells in culture, in vitro or ex vivo, or to human subjects, e.g., in vivo, to treat, prevent and to diagnose a variety of disorders. As used herein, the term "subject" is intended to include human and non-human animals. Non-human animals include all vertebrates, e.g., mammals and non-mammals, such as non-human primates, sheep, dogs, cats, cows, horses, chickens, amphibians, and reptiles. Preferred subjects include human patients having disorders mediated by CD 19 activity. The methods are particularly suitable for treating human patients having a disorder associated with aberrant CD 19 expression. When antibody-partner molecule conjugates to CD 19 are administered together with another agent, the two can be administered in either order or simultaneously.
Given the specific binding of the antibodies of this disclosure for CD 19, the antibodies of this disclosure can be used to specifically detect CD 19 expression on the surface of cells and, moreover, can be used to purify CD 19 via immunoaffϊnity purification.
Furthermore, given the expression of CD 19 on various tumor cells, the human antibody-partner molecule conjugate compositions and methods of the present disclosure can be used to treat a subject with a tumorigenic disorder, e.g., a disorder characterized by the presence of tumor cells expressing CD19 including, for example, non-Hodgkin's lymphoma (NHL), acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL), Burkitt's lymphoma, anaplastic large-cell lymphomas (ALCL), multiple myeloma, cutaneous T-cell lymphomas, nodular small cleaved-cell lymphomas, lymphocytic lymphomas, peripheral T-cell lymphomas, Lennert's lymphomas, immunoblastic lymphomas, T-cell leukemia/lymphomas (ATLL), adult T-cell leukemia (T-ALL), entroblastic/centrocytic (cb/cc) follicular lymphomas cancers, diffuse large cell lymphomas of B lineage, angioimmunoblastic lymphadenopathy (AILD)-like T cell lymphoma, HIV associated body cavity based lymphomas, Embryonal Carcinomas, undifferentiated carcinomas of the rhino-pharynx (e.g., Schmincke's tumor), Castleman's disease, Kaposi's Sarcoma, Multiple Myeloma, Waldenstrom's macroglobulinemia, and other B-cell lymphomas.
Additionally, overexpression of CD 19 may lead to loss of B-cell tolerance and generation of autoimmune disorders (Tedder et al. (2005) Curr Dir Autoimmun 8:55). This autoimmune effect has been seen by the accumulation of CD 19+ B-cells in the inflamed joints of rheumatoid arthritis patients (He et al. (2001 ) J Rheumatol 28:2168). As such, the human antibodies, antibody compositions and methods of the present disclosure can be used to treat a subject with an autoimmune disorder, e.g., a disorder characterized by the presence of B-cells expressing CD 19 including, for example, rheumatoid arthritis.
In one embodiment, the antibodies (e.g., human monoclonal antibodies, multispecific and bispecific molecules and compositions) of this disclosure can be used to detect levels of CD 19, or levels of cells which contain CD 19 on their membrane surface, which levels can then be linked to certain disease symptoms. Alternatively, the antibodies can be used to inhibit or block CD 19 function which, in turn, can be linked to the prevention or amelioration of certain disease symptoms, thereby implicating CD 19 as .a mediator of the disease. This can be achieved by contacting a sample and a control sample with the anti-CD 19 antibody under conditions that allow for the formation of a complex between the antibody and CD 19. Any complexes formed between the antibody and CD 19 are detected and compared in the sample and the control. In another embodiment, the antibodies (e.g., human antibodies, multispecifϊc and bispecific molecules and compositions) of this disclosure can be initially tested for binding activity associated with therapeutic or diagnostic use in vitro. For example, compositions of this disclosure can be tested using the flow cytometric assays described in the Examples below. The antibodies (e.g., human antibodies, multispecific and bispecific molecules, immunoconjugates and compositions) of this disclosure have additional utility in therapy and diagnosis of CD19-related diseases. For example, the human monoclonal antibodies, the multispecific or bispecific molecules and the immunoconjugates can be used to elicit in vivo or in vitro one or more of the following biological activities: to inhibit the growth of and/or kill a cell expressing CDl 9; to mediate phagocytosis or ADCC of a cell expressing CD 19 in the presence of human effector cells, or to block CD 19 ligand binding to CD 19.
In a particular embodiment, the antibodies (e.g., human antibodies, multispecific and bispecific molecules and compositions) are used in vivo to treat, prevent or diagnose a variety of CD 19-related diseases. Examples of CD 19-related diseases include, among others, autoimmune disorders, rheumatoid arthritis, cancer, non-Hodgkin's lymphoma, acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL), Burkitt's lymphoma, anaplastic large-cell lymphomas (ALCL), multiple myeloma, cutaneous T- cell lymphomas, nodular small cleaved-cell lymphomas, lymphocytic lymphomas, peripheral T-cell lymphomas, Lennert's lymphomas, immunoblastic lymphomas, T-cell leukemia/lymphomas (ATLL), adult T-cell leukemia (T-ALL), entroblastic/centrocytic (cb/cc) follicular lymphomas cancers, diffuse large cell lymphomas of B lineage, angioimmunoblastic lymphadenopathy (AILD)-like T cell lymphoma, HIV associated body cavity based lymphomas, Embryonal Carcinomas, undifferentiated carcinomas of the rhino-pharynx (e.g., Schmincke's tumor), Castleman's disease, Kaposi's Sarcoma, Multiple Myeloma, Waldenstrom's macroglobulinemia, and other B-cell lymphomas. Suitable routes of administering the antibody compositions of this disclosure (e.g., human monoclonal antibodies, multispecific and bispecific molecules and immunoconjugates) in vivo and in vitro are well known in the art and can be selected by those of ordinary skill. For example, the antibody compositions can be administered by injection (e.g., intravenous or subcutaneous). Suitable dosages of the molecules used will depend on the age and weight of the subject and the concentration and/or formulation of the antibody composition.
As previously described, human anti-CD 19 antibodies of this disclosure can be co-administered with one or other more therapeutic agents, e.g., a cytotoxic agent, a radiotoxic agent or an immunosuppressive agent. The antibody can be linked to the agent (as an immunocomplex) or can be administered separate from the agent. In the latter case (separate administration), the antibody can be administered before, after or concurrently with the agent or can be co-administered with other known therapies, e.g., an anti-cancer therapy, e.g., radiation. Such therapeutic agents include, among others, anti-neoplastic agents such as doxorubicin (adriamycin), cisplatin bleomycin sulfate, carmustine, chlorambucil, and cyclophosphamide hydroxyurea which, by themselves, are only effective at levels which are toxic or subtoxic to a patient. Cisplatin is intravenously administered as a 100 mg/ dose once every four weeks and adriamycin is intravenously administered as a 60-75 mg/ml dose once every 21 days. Co-administration of the human anti-CD 19 antibodies, or antigen binding fragments thereof, of the present disclosure with chemotherapeutic agents provides two anti-cancer agents which operate via different mechanisms which yield a cytotoxic effect to human tumor cells. Such co-administration can solve problems due to development of resistance to drugs or a change in the antigenicity of the tumor cells which would render them unreactive with the antibody. Target-specific effector cells, e.g., effector cells linked to compositions (e.g., human antibodies, multispecific and bispecific molecules) of this disclosure can also be used as therapeutic agents. Effector cells for targeting can be human leukocytes such as macrophages, neutrophils or monocytes. Other cells include eosinophils, natural killer cells and other IgG- or IgA-receptor bearing cells. If desired, effector cells can be obtained from the subject to be treated. The target-specific effector cells can be administered as a suspension of cells in a physiologically acceptable solution. The number of cells administered can be in the order of 10s- 109 but will vary depending on the therapeutic purpose. In general, the amount will be sufficient to obtain localization at the target cell, e.g., a tumor cell expressing CD 19, and to effect cell killing by, e.g., phagocytosis. Routes of administration can also vary. Therapy with target-specific effector cells can be performed in conjunction with other techniques for removal of targeted cells. For example, anti-tumor therapy using the compositions (e.g., human antibodies, multispecifϊc and bispecific molecules) of this disclosure and/or effector cells armed with these compositions can be used in conjunction with chemotherapy. Additionally, combination immunotherapy may be used to direct two distinct cytotoxic effector populations toward tumor cell rejection. For example, anti-CD 19 antibodies linked to anti-Fc-gamma RI or anti-CD3 may be used in conjunction with IgG- or IgA-receptor specific binding agents.
Bispecific and multispecific molecules of this disclosure can also be used to modulate FcγR or FcγR levels on effector cells, such as by capping and elimination of receptors on the cell surface. Mixtures of anti-Fc receptors can also be used for this purpose.
The compositions {e.g., human antibodies, multispecific and bispecific molecules and immunoconjugates) of this disclosure which have complement binding sites, such as portions from IgGl, -2, or -3 or IgM which bind complement, can also be used in the presence of complement. In one embodiment, ex vivo treatment of a population of cells comprising target cells with a binding agent of this disclosure and appropriate effector cells can be supplemented by the addition of complement or serum containing complement. Phagocytosis of target cells coated with a binding agent of this disclosure can be improved by binding of complement proteins. In another embodiment target cells coated with the compositions (e.g., human antibodies, multispecific and bispecific molecules) of this disclosure can also be lysed by complement. In yet another embodiment, the compositions of this disclosure do not activate complement.
The compositions of this disclosure (e.g., human antibodies, multispecific and bispecific molecules and immunoconjugates) can also be administered together with complement. In certain embodiments, the instant disclosure provides compositions comprising human antibodies, multispecific or bispecific molecules and serum or complement. These compositions can be advantageous when the complement is located in close proximity to the human antibodies, multispecific or bispecific molecules. Alternatively, the human antibodies, multispecific or bispecific molecules of this disclosure and the complement or serum can be administered separately. Also within the scope of the present disclosure are kits, which comprise the antibody compositions of this disclosure (e.g., human antibodies, bispecific or multispecific molecules, or immunoconjugates), and instructions for its use. The kit can further contain one or more additional reagents, such as an immunosuppressive reagent, a cytotoxic agent or a radiotoxic agent, or one or more additional human antibodies of this disclosure (e.g., a human antibody having a complementary activity which binds to an epitope in the CD 19 antigen distinct from the first human antibody).
Accordingly, patients treated with antibody compositions of this disclosure can be additionally administered (prior to, simultaneously with, or following administration of a human antibody of this disclosure) with another therapeutic agent, such as a cytotoxic or radiotoxic agent, which enhances or augments the therapeutic effect of the human antibodies.
In other embodiments, the subject can be additionally treated with an agent that modulates, e.g., enhances or inhibits, the expression or activity of Fcγ or Fcγ receptors by, for example, treating the subject with a cytokine. Preferred cytokines for administration during treatment with the multispecific molecule include of granulocyte colony-stimulating factor (G-CSF), granulocyte- macrophage colony-stimulating factor (GM-CSF), interferon-γ (IFN-γ), and tumor necrosis factor (TNF).
The compositions (e.g., human antibodies, multispecific and bispecific molecules) of this disclosure can also be used to target cells expressing FcγR or CD 19, for example for labeling such cells. For such use, the binding agent can be linked to a molecule that can be detected. Thus, this disclosure provides methods for localizing ex vivo or in vitro cells expressing Fc receptors, such as FcγR, or CD 19. The detectable label can be, e.g., a radioisotope, a fluorescent compound, an enzyme, or an enzyme co-factor.
In a particular embodiment, this disclosure provides methods for detecting the presence of CD 19 antigen in a sample, or measuring the amount of CD 19 antigen, comprising contacting the sample, and a control sample, with a human monoclonal antibody, or an antigen binding portion thereof, which specifically binds to CD 19, under conditions that allow for formation of a complex between the antibody or portion thereof and CD 19. The formation of a complex is then detected, wherein a difference complex formation between the sample compared to the control sample is indicative the presence of CD 19 antigen in the sample.
In other embodiments, this disclosure provides methods for treating an CD 19 mediated disorder in a subject, e.g., autoimmune disorder, rheumatoid arthritis, cancer, non-Hodgkin's lymphoma, acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL), Burkitt's lymphoma, anaplastic large-cell lymphomas (ALCL), cutaneous T-cell lymphomas, nodular small cleaved-cell lymphomas, lymphocytic lymphomas, peripheral T-cell lymphomas, Lennert's lymphomas, immunoblastic lymphomas, T-cell leukemia/lymphomas (ATLL), adult T-cell leukemia (T-ALL), entroblastic/centrocytic (cb/cc) follicular lymphomas cancers, diffuse large cell lymphomas of B lineage, angioimmunoblastic lymphadenopathy (AILD)-like T cell lymphoma, HIV associated body cavity based lymphomas, Embryonal Carcinomas, undifferentiated carcinomas of the rhino-pharynx (e.g., Schmincke's tumor), Castleman's disease, Kaposi's Sarcoma, Multiple Myeloma, Waldenstrom's macroglobulinemia, and other B-cell lymphomas, by administering to the subject the human antibodies described above. Such antibodies and derivatives thereof are used to inhibit CD 19 induced activities associated with certain disorders, e.g., proliferation and differentiation. By contacting the antibody with CD 19 (e.g., by administering the antibody to a subject), the ability of CD 19 to induce such activities is inhibited and, thus, the associated disorder is treated. The antibody composition can be administered alone or along with another therapeutic agent, such as a cytotoxic or a radiotoxic agent which acts in conjunction with or synergistically with the antibody composition to treat or prevent the CD 19 mediated disease.
In yet another embodiment, immunoconjugates of this disclosure can be used to target compounds (e.g., therapeutic agents, labels, cytotoxins, radiotoxins immunosuppressants, etc.) to cells which have CD 19 cell surface receptors by linking such compounds to the antibody. For example, an anti-CD 19 antibody can be conjugated to any of the toxin compounds described in US Patent Nos. 6, 281, 354 and 6,548,530, US patent publication Nos. 20030050331, 20030064984, 20030073852, and 20040087497, or published in WO 03/022806. Thus, this disclosure also provides methods for localizing ex vivo or in vivo cells expressing CD 19 (e.g., with a detectable label, such as a radioisotope, a fluorescent compound, an enzyme, or an enzyme co- factor). Alternatively, the immunoconjugates can be used to kill cells which have CD 19 cell surface receptors by targeting cytotoxins or radiotoxins to CD 19.
The present disclosure is further illustrated by the following examples which should not be construed as further limiting. The contents of all figures and all references, Genbank sequences, patents and published patent applications cited throughout this application are expressly incorporated herein by reference in their entirety.
EXAMPLES
Example 1 : Generation of Human Monoclonal Antibodies Against CD19
Antigen
The B cell tumor cell lines Raji (ATCC Accession #CCL-86) and Daudi (ATCC Accession #CCL-213) were used as antigen for immunization.
Transgenic Transchromosomic KM-MOUSE®
Fully human monoclonal antibodies to CD 19 were prepared using the KM strain of transgenic transchromosomic mice, which expresses human antibody genes. In this mouse strain, the endogenous mouse kappa light chain gene has been homozygously disrupted as described in Chen et al. ( 1993) EMBO J. 12:81 1 -820 and the endogenous mouse heavy chain gene has been homozygously disrupted as described in Example 1 of PCT Publication WO 01/09187 for HuMab mice. The mouse carries a human kappa light chain transgene, KCo5, as described in Fishwild et al. (1996) Nature Biotechnology 14:845-851. The mouse also carries a human heavy chain transchromosome, SC20, as described in PCT Publication WO 02/43478.
KM-MOUSE® Immunizations:
To generate fully human monoclonal antibodies to CD 19, cohorts of the KM- MOUSE® were immunized with either the Raji or Daudi B cell tumor cell line. General immunization schemes are described in Lonberg, N. et al. (1994) Nature 368(6474): 856- 859; Fishwild, D. et al. (1996) Nature Biotechnology J4: 845-851 and PCT Publication WO 98/24884. The mice were 6-16 weeks of age upon the first infusion of antigen. A cell preparation was used to immunize the mice (KM-MOUSE®) intraperitonealy (IP).
Transgenic mice were immunized twice with antigen in complete Freund's adjuvant or Ribi adjuvant IP, followed by 3-21 days IP (up to a total of 1 1 immunizations) with the antigen in incomplete Freund's or Ribi adjuvant. The immune response was monitored by retroorbital bleeds. The plasma was screened by ELISA (as described below), and mice with sufficient titers of anti-CD 19 human immunogolobulin were used for fusions. Mice were boosted intravenously with antigen 3 days before sacrifice and removal of the spleen. Selection of a KM-MOUSE® Producing Anti-CD 19 Antibodies:
To select a KM-MOUSE® producing antibodies that bound CD 19, sera from immunized mice were tested by a modified ELISA as originally described by Fishwild, D. et al, (1996), supra. Briefly, microtiter plates were coated with purified recombinant CD 19 fusion protein at 1-2 μg /ml in PBS, 50 μl/wells incubated 4 0C overnight then blocked with 200 μl/well of 5% BSA in PBS. Dilutions of plasma from CD 19- immunized mice were added to each well and incubated for 1-2 hours at ambient temperature. The plates were washed with PBS/Tween and then incubated with a goat- anti-human kappa light chain polyclonal antibody conjugated with alkaline phophatase for 1 hour at room temperature. After washing, the plates were developed with pNPP substrate and analyzed by spectrophotometer at OD 415-650. Mice that developed the highest titers of anti-CD 19 antibodies were used for fusions. Fusions were performed as described below and hybridoma supernatants were tested for anti-CD 19 activity by ELISA. Generation of Hybridomas Producing Human Monoclonal Antibodies to CD 19:
The mouse splenocytes, isolated from a KM-MOUSE*", were fused with PEG to a mouse myeloma cell line either using PEG based upon standard protocols or electric field based electrofusion using a Cyto Pulse large chamber cull fusion electroporator (Cyto Pulse Sciences, Inc., Glen Burnie, MD). The resulting hybridomas were then screened for the production of antigen-specific antibodies. Single cell suspensions of splenic lymphocytes from immunized mice were fused to one-fourth the number of SP2/0 nonsecreting mouse myeloma cells (ATCC, CRL 1581) with 50% PEG (Sigma). Cells were plated at approximately lxlO5/well in flat bottom microtiter plate, followed by about two week incubation in selective medium containing 10% fetal bovine serum, 10% P388D1 (ATCC, CRL TIB-63) conditioned medium, 3-5% origen (IGEN) in DMEM (Mediatech, CRL 10013, with high glucose, L-glutamine and sodium pyruvate) plus 5 mM HEPES, 0.055 mM 2-mercaptoethanol, 50 mg/ml gentamycin and Ix HAT (Sigma, CRL P-7185). After 1-2 weeks, cells were cultured in medium in which the HAT was replaced with HT. Individual wells were then screened by ELISA (described above) for human anti-CD 19 monoclonal IgG antibodies. Once extensive hybridoma growth occurred, medium was monitored usually after 10-14 days. The antibody secreting hybridomas were replated, screened again and, if still positive for human IgG, anti-CD 19 monoclonal antibodies were subcloned at least twice by limiting dilution. The stable subclones were then cultured in vitro to generate small amounts of antibody in tissue culture medium for further characterization. Hybridoma clones 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 were selected for further analysis.
Example 2: Structural Characterization of Human Monoclonal Antibodies 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8
The cDNA sequences encoding the heavy and light chain variable regions of the 21D4 and 21D4a monoclonal antibodies were obtained from the 21D4 hybridoma using standard PCR techniques and were sequenced using standard DNA sequencing techniques. It is noted that the 21D4 hybridoma produces antibodies having a heavy chain that pairs with one of two light chains (SEQ ID NOs: 8 and 9). Both antibodies (i.e., 21D4 with VH and VL sequences of SEQ ID NOs: 1 and 8, respectively, and 21D4a with VH and V1 sequences of SEQ ID NOs: 1 and 9, respectively) bind to CD 19. The cDNA sequences encoding the heavy and light chain variable regions of the 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 monoclonal antibodies were obtained from the 21D4, 21D4a, 47G4, 27F3, 3C10, 5G7, 13Fl and 46E8 hybridomas, respectively, using standard PCR techniques and were sequenced using standard DNA sequencing techniques. The nucleotide and amino acid sequences of the heavy chain variable region of
21D4 are shown in Figure IA and in SEQ ID NO: 59 and 1, respectively.
The nucleotide and amino acid sequences of the light chain variable region of 21D4 are shown in Figure IB and in SEQ ID NO: 66 and 8, respectively.
Comparison of the 21D4 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 21 D4 heavy chain utilizes a VH segment from human germline VH 5-51, a D segment from the human germline 3-10, and a JH segment from human germline JH 4b. The alignment of the 21D4 VH sequence to the germline VH 5-51 sequence is shown in Figure 8. Further analysis of the 21D4 Vn sequence using the Kabat system of CDR region determination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures IA and 8, and in SEQ ID NOs: 16, 23 and 30, respectively.
Comparison of the 21D4 light chain immunoglobulin sequence to the known human germline immunoglobulin light chain sequences demonstrated that the 21D4 light chain utilizes a VL segment from human germline Vκ Ll 8 and a JK segment from human germline JK 2. The alignment of the 21 D4 VL sequence to the germline VK Ll 8 sequence is shown in Figure 15. Further analysis of the 21D4 VL sequence using the Kabat system of CDR region determination led to the delineation of the light chain CDRl, CDR2 and CD3 regions as shown in Figures IB and 15, and in SEQ ID NOs: 37, 44 and 51 , respectively.
The nucleotide and amino acid sequences of the heavy chain variable region of 21D4a are shown in Figure IA and in SEQ ID NO: 59 and 1, respectively.
The nucleotide and amino acid sequences of the light chain variable region of 21D4a are shown in Figure 1C and in SEQ ID NO: 67 and 9, respectively. Comparison of the 21D4a heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 21D4a heavy chain utilizes a Vn segment from human germline VH 5-51, a D segment from the human germline 3-10, and a JH segment from human germline JH 4b. The alignment of the 21D4a VH sequence to the germline V( 1 5-51 sequence is shown in Figure 8. Further analysis of the 21 D4a VH sequence using the Kabat system of CDR region determination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures IA and 8, and in SEQ ID NOs: 16, 23 and 30, respectively.
Comparison of the 21D4a light chain immunoglobulin sequence to the known human germline immunoglobulin light chain sequences demonstrated that the 21D4a light chain utilizes a VL segment from human germline VK Ll 8 and a JK segment from human germline JK 3. The alignment of the 21 D4a Vt sequence to the germline Vκ Ll 8 sequence is shown in Figure 16. Further analysis of the 2 lD4a V[, sequence using the Kabat system of CDR region determination led to the delineation of the light chain CDRl, CDR2 and CD3 regions as shown in Figures 1C and 16, and in SEQ ID NOs: 37, 44 and 52, respectively. The nucleotide and amino acid sequences of the heavy chain variable region of 47G4 are shown in Figure 2A and in SEQ ID NO: 60 and 2, respectively.
The nucleotide and amino acid sequences of the light chain variable region of 47G4 are shown in Figure 2B and in SEQ ID NO: 68 and 10, respectively. Comparison of the 47G4 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 47G4 heavy chain utilizes a VH segment from human germline VH 1-69, a D segment from the human germline 6-19, and a JH segment from human germline JH 5b. The alignment of the 47G4 VH sequence to the germline VH 1 -69 sequence is shown in Figure 9. Further analysis of the 47G4 VH sequence using the Kabat system of CDR region determination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures 2A and 9, and in SEQ ID NOs: 17, 24 and 31 , respectively.
Comparison of the 47G4 light chain immunoglobulin sequence to the known human germline immunoglobulin light chain sequences demonstrated that the 47G4 light chain utilizes a VL segment from human germline Vκ A27 and a JK segment from human germline JK 3. The alignment of the 47G4 V1 sequence to the germline Vκ A27 sequence is shown in Figure 17. Further analysis of the 47G4 VL sequence using the Kabat system of CDR region determination led to the delineation of the light chain CDRl, CDR2 and CD3 regions as shown in Figures 2B and 17, and in SEQ ID NOs: 38, 45 and 53, respectively.
The nucleotide and amino acid sequences of the heavy chain variable region of 27F3 are shown in Figure 3A and in SEQ ID NO: 61 and 3, respectively.
The nucleotide and amino acid sequences of the light chain variable region of 27F3 are shown in Figure 3B and in SEQ ID NO: 69 and 1 1, respectively. Comparison of the 27F3 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 27F3 heavy chain utilizes a VH segment from human germline VH 5-51, a D segment from the human germline 6-19, and a JH segment from human germline JH 6b. The alignment of the 27F3 VH sequence to the germline VH 5-51 sequence is shown in Figure 10. Further analysis of the 27F3 VH sequence using the Kabat system of CDR region determination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures 3A and 10, and in SEQ ID NOs: 18, 25 and 32, respectively.
Comparison of the 27F3 light chain immunoglobulin sequence to the known human germline immunoglobulin light chain sequences demonstrated that the 27F3 light chain utilizes a V[ segment from human germline VK Ll 8 and a Jκ segment from human germline JK 2. The alignment of the 27F3 V1, sequence to the germline VK Ll 8 sequence is shown in Figure 18. Further analysis of the 27F3 V1 sequence using the Kabat system of CDR region determination led to the delineation of the light chain CDRl, CDR2 and CD3 regions as shown in Figures 3B and 18, and in SEQ ID NOs: 39, 46 and 54, respectively.
The nucleotide and amino acid sequences of the heavy chain variable region of 3C10 are shown in Figure 4A and in SEQ ID NO: 62 and 4, respectively.
The nucleotide and amino acid sequences of the light chain variable region of 3ClO are shown in Figure 4B and in SEQ ID NO: 70 and 12, respectively. Comparison of the 3C10 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 3C10 heavy chain utilizes a VH segment from human germline VH 1-69, a D segment from the human germline 1-26, and a JH segment from human germline JH 6b. The alignment of the 3C10 VH sequence to the geπnline VH 1-69 sequence is shown in Figure 1 1. Further analysis of the 3C10 VH sequence using the Kabat system of CDR region determination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures 4A and 1 1, and in SEQ ID NOs: 19, 26 and 33, respectively.
Comparison of the 3C10 light chain immunoglobulin sequence to the known human germline immunoglobulin light chain sequences demonstrated that the 3C10 light chain utilizes a VL segment from human geπnline VK Ll 5 and a JK segment from human germline JK 2. The alignment of the 3C10 VL sequence to the geπnline Vκ L15 sequence is shown in Figure 19. Further analysis of the 3C 10 VL sequence using the Kabat system of CDR region deteπnination led to the delineation of the light chain CDRl, CDR2 and CD3 regions as shown in Figures 4B and 19, and in SEQ ID NOs: 40, 47 and 55, respectively. The nucleotide and amino acid sequences of the heavy chain variable region of 5G7 are shown in Figure 5A and in SEQ ID NO: 63 and 5, respectively.
The nucleotide and amino acid sequences of the light chain variable region of 5G7 are shown in Figure 5B and in SEQ ID NO: 71 and 13, respectively. Comparison of the 5G7 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 5G7 heavy chain utilizes a VH segment from human germline VH 5-51, a D segment from the human germline 3-10, and a JH segment from human germline JH 6b. The alignment of the 5G7 VH sequence to the germline VH 5-51 sequence is shown in Figure 12. Further analysis of the 5G7 VH sequence using the Kabat system of CDR region determination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures 5 A and 12, and in SEQ ID NOs: 20, 27 and 34, respectively.
Comparison of the 5G7 light chain immunoglobulin sequence to the known human germline immunoglobulin light chain sequences demonstrated that the 5G7 light chain utilizes a VL segment from human germline Vκ Ll 8 and a JK segment from human germline JK 1. The alignment of the 5G7 V1 sequence to the germline VK Ll 8 sequence is shown in Figure 20. Further analysis of the 5G7 VL sequence using the Kabat system of CDR region determination led to the delineation of the light chain CDRl, CDR2 and CD3 regions as shown in Figures 5B and 20, and in SEQ ID NOs: 41 , 48 and 56, respectively.
The nucleotide and amino acid sequences of the heavy chain variable region of 13Fl are shown in Figure 6A and in SEQ ID NO: 64 and 6, respectively.
The nucleotide and amino acid sequences of the light chain variable region of 13Fl are shown in Figure 6B and in SEQ ID NO: 72 and 14, respectively. Comparison of the 13Fl heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 13Fl heavy chain utilizes a VH segment from human germline VH 5-51, a D segment from the human germline 6-19, and a Jn segment from human germline JH 6b. The alignment of the 13Fl VH sequence to the germline Vn 5-51 sequence is shown in Figure 13. Further analysis of the 13Fl VH sequence using the Kabat system of CDR region determination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures 6A and 13, and in SEQ ID NOs: 21, 28 and 35, respectively.
Comparison of the 13Fl light chain immunoglobulin sequence to the known human germline immunoglobulin light chain sequences demonstrated that the 13Fl light chain utilizes a Vt segment from human germline VK Ll 8 and a JK segment from human germline JK 2. The alignment of the 13Fl VL sequence to the germline VK L18 sequence is shown in Figure 21. Further analysis of the 13Fl Vt sequence using the Kabat system of CDR region determination led to the delineation of the light chain CDRl, CDR2 and CD3 regions as shown in Figures 6B and 21, and in SEQ ID NOs: 42, 49 and 57, respectively.
The nucleotide and amino acid sequences of the heavy chain variable region of 46E8 are shown in Figure 7A and in SEQ ID NO: 65 and 7, respectively.
The nucleotide and amino acid sequences of the light chain variable region of 46E8 are shown in Figure 7B and in SEQ ID NO: 73 and 15, respectively. Comparison of the 46E8 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 46E8 heavy chain utilizes a VH segment from human germline VH 5-51 , a D segment from the human germline 6-19, and a JH segment from human germline JH 6b. The alignment of the 46E8 Vn sequence to the germline VH 5-51 sequence is shown in Figure 14. Further analysis of the 46E8 Vn sequence using the Kabat system of CDR region deteπnination led to the delineation of the heavy chain CDRl, CDR2 and CD3 regions as shown in Figures 7A and 14, and in SEQ ID NOs: 22, 29 and 36, respectively.
Comparison of the 46E8 light chain immunoglobulin sequence to the known human germline immunoglobulin light chain sequences demonstrated that the 46E8 light chain utilizes a V1 segment from human germline VK Ll 8 and a JK segment from human germline JK 2. The alignment of the 46E8 VL sequence to the germline Vκ Ll 8 sequence is shown in Figure 22. Further analysis of the 46E8 Vt sequence using the Kabat system of CDR region deteπnination led to the delineation of the light chain CDRl, CDR2 and CD3 regions as shown in Figures 7B and 22, and in SEQ ID NOs: 43, 50 and 58, respectively. Example 3: Characterization of Binding Specificity and Binding Kinetics of Anti- CD^ Human Monoclonal Antibodies
In this example, the binding affinity of the anti-CD 19 antibodies 21D4 and 47G4 were examined by ELISA analysis. Binding specificity by ELISA
Microtiter plates were coated with 50 μl purified full-length CD 19-Fc fusion protein at 1.0 μg/ml in PBS, and then blocked with 150 μl of 1% bovine serum albumin in PBS. The plates were allowed to incubate for 30 minutes to 1 hour and washed three times. Dilutions of the HuMAb anti-CD 19 antibody 47G4 was added to each well and incubated for 1 hour at 370C. A known murine anti-CD 19 antibody was used as a positive control. The plates were washed with PBS/Tween and then incubated with a goat anti-human IgG Kappa-specific secondary reagent conjugated to horseradish peroxidase for 1 hour at 370C. After washing, the plates were developed with ABTS substrate (1.46 mMol/L), and analyzed at OD of 490 nm. The results are depicted in Figure 23. The CD 19 HuMAb 47G4 specifically bound to the human CD 19 peptide.
Epitope Mapping of anti-CD 19 Antibodies
Flow cytometry was used to determine epitope grouping of anti-CD 19 HuMAbs. Epitope binding of the anti-CD 19 human monoclonal antibodies 21D4, 21D4a, 3C10, 5G7, 5G7-N19K, 5G7-N19Q and 13Fl was assessed by incubating Raji B tumor cells with 0.3 μg/ml of either biotinylated 21 D4 or 21 D4a anti-CD 19 human monoclonal antibody, washed, and followed by the addition of a cold anti-CD 19 human monoclonal antibody. An isotype control antibody was used as a negative control. Binding was detected with a FITC-labeled anti-human IgG Ab. Flow cytometric analyses were performed using a FACScan flow cytometry (Becton Dickinson, San Jose, CA). The results are shown in Figure 24A and B. Upon analysis of the data, the anti-CD 19 antibodies 21 D4, 21D4a, 3C10, 5G7 and 13Fl compete for the same epitope region.
Example 4: Binding of the CD19 Antibodies to a B cell-Derived Tumor Cell Line
Binding of the CD 19 HuMAbs by flow cytometry to the B cell tumor lines Raji and Daudi, or to a CHO-CD 19 transfected cell line was assessed. CHO cells were transfected with an expression plasmid containing the full length cDNA encoding the transmembrane form of CD 19. The Raji, Daudi, and CD19-CHO cell lines were incubated with one of the following CD 19 HuMAbs: 21D4, 21D4a, 47G4, 5G7, 5G7- N 19K, 5G7-N19Q, 3C10 or 13Fl . A known murine anti-CD 19 antibody was used as a positive control. The cells were washed and detected by either a phycoerythrin-labeled anti-human or anti-mouse secondary antibody and analyzed by flow cytometry. The results for binding to the CHO-CD 19 cell line, Daudi B cell line, Raji B cell line and an expanded binding set against the Raji B cell line are shown in Figures 25 A, 25B, 25C and 25D, respectively. The human anti-CD 19 monoclonal antibodies, 21D4 and 47G4, bound to the CHO-CD 19 cell line. The human anti-CD 19 monoclonal antibodies, 21 D4, 21D4a, 47G4, 5G7, 5G7-N19K, 5G7-N19Q, 3C10 and 13Fl, bound to the Raji B cell line. The anti-CD19 HuMAb antibodies 21 D4, 21D4a, 3C10, 5G7, 5G7-N19K, 5G7- N19Q, and 13Fl had calculated EC50 values of 0.1413, 0.1293, 0.2399, 0.1878. 0.2240, 0.2167 and 0.2659, respectively. 47G4 was also shown to bind the Daudi B tumor cell line. AU results are shown as measured by the geometric mean fluorescent intensity (GMFI) of staining. These data show that the CD 19 protein is expressed on the surface of tumor cell lines of B cell origin and that the anti-CD 19 HuMAb antibodies 21D4, 21D4a, 47G4, 5G7, 5G7-N19K, 5G7-N19Q, 3C10 and 13Fl bind to CD19 expressed on the cell surface.
Example 5: Scatchard Binding Analysis of the Anti-CD19 Human Antibodies 21D4 and 47G4 to Raji B Tumor Cells
Raji cells were obtained from ATCC (Accession #CCL-86) and grown in RPMI containing 10% fetal bovine serum (FBS). The cells were washed twice with RPMI containing 10% FBS at 4 0C and the cells were adjusted to 4x107 cells/ml in RPMI media containing 10% fetal bovine serum (binding buffer containing 24mM Tris pH 7.2, 137mM NaCl, 2.7mN KCl, 2mM glucose, ImM CaCl2, ImM MgCl2, 0.1% BSA). Millipore plates (MAFB NOB) were coated with 1% nonfat dry milk in water and stored a 4 0C overnight. The plates were washed with binding buffer and 25 μl of unlabeled antibody (1000-fold excess) in binding buffer was added to control wells in a Millipore 96 well glass fiber filter plate (non-specific binding NSB). Twenty-five microliters of buffer alone was added to the maximum binding control well (total binding). Twenty- five microliters of varying concentrations of l2:>I-anti-CD19 antibody 21D4 or 47G4 and 25 μl of Raji cells (4 X 107 cells/ml) in binding buffer were added. The plates were incubated for 2 hours at 200 RPM on a shaker at 40C. At the completion of the incubation the Millipore plates were washed three times with 0.2 ml of cold wash buffer (24mM Tris pH 7.2, 50OmM NaCl, 2.7mN KCl, 2mM glucose, ImM CaCl2, ImM MgCl2, 0.1% BSA). The filters were removed and counted in a gamma counter. Evaluation of equilibrium binding was performed using single site binding parameters with the Prism software (San Diego, CA). Using the above scatchard binding assay, the KD of the antibody for Raji cells was approximately 2.14 nM for 21D4 and 12.02 nM for 47G4.
Example 6: Internalization of Anti-CD19 Monoclonal Antibody
Anti-CD 19 HuMAbs were tested for the ability to internalize into CD 19- expressing Raji B tumor cells or human CHO cells transfected with CD 19 using a Hum- Zap internalization assay. Hum-Zap tests for internalization of a primary human antibody through binding of a secondary antibody with affinity for human IgG conjugated to the toxin saporin.
The CHO-CD 19 or Raji B tumor cell line was seeded at 1.0x104 cells/well in 100 μl wells either overnight or the following day for a two hour period. Either the anti- CD 19 antibody 21 D4 or 47G4 were added to the wells at a starting concentration of 30 nM and titrated down at 1:3 serial dilutions. A human isotype control antibody that is non-specific for CD 19 was used as a negative control. The Hum-Zap (Advanced Targeting Systems, IT-22-25) was added at a concentration of 11 nM and plates were allowed to incubate for 48 hours. The plates were then pulsed with 1.0 μCi of JH- thymidine for 18-24 hours, harvested and read in a Top Count Scintillation Counter
(Packard Instruments). The results for internalization on CHO-CD 19 and B tumor cells are shown in Figures 26A and 26B, respectively. Only the HuMAb 47G4 was tested on CHO-CD 19 cells. The anti-CD 19 antibody 47G4 showed an antibody concentration dependent decrease in Η-thymidine incorporation on CHO-CD 19 cells. Both the 21 D4 and 47G4 HuMAbs showed an antibody concentration dependent decrease in 3H- thymidine incoiporation on Raji B tumor cells. This data demonstrates that the anti- CD 19 antibodies 21D4 and 47G4 internalize into CD 19 expressing CHO-CD 19 transfectant cells and B tumor cells.
Example 7: Assessment of Cell Killing of a Cytotoxin-Conjugated Anti-CD 19 Antibody
In this example, anti-CD 19 monoclonal antibodies conjugated to a cytotoxin were tested for the ability to kill CD 19+ cell lines in a thymidine incorporation assay. Cytotoxin N 1 was used in this experiment. An anti-CD 19 monoclonal antibody was conjugated to a cytotoxin via a linker, such as a peptidyl, hydrazone or disulfide linker. The CD 19+ expressing Raji cell line was seeded at 2.5x104 cells/wells for 3 hours. An anti-CD 19 antibody-cytotoxin conjugate was added to the wells at a starting concentration of 30 nM and titrated down at 1 :3 serial dilutions. An isotype control antibody that is non-specific for CDl 9 was used as a negative control. Ten-fold excess cold antibody, either 21 D4a or an isotype control antibody is used to compete binding. Plates were allowed to incubate for 69 hours. The plates were then pulsed with 1.0 μCi of 3H-thymidine for 24 hours, harvested and read in a Top Count Scintillation Counter (Packard Instruments, Meriden, CT). The results are shown in Figure 27A and B along with the EC50 values. This data demonstrates that the anti-CD 19 antibody 21 D4 kills Raji B-cell tumor cells.
Example 8: Treatment of in vivo B cell Tumors Using Anti-CD19 Antibodies
In this Example, SCID mice implanted with cancerous B cell tumors were treated in vivo with either naked anti-CD19 21D4 antibodies or cytotox in-conjugated anti-CD19 antibody 21D4 to examine the in vivo effect of the antibodies on tumor growth. Cytotoxin N 1 was used in this experiment.
Cytotoxin-conjugated anti-CD 19 antibodies were prepared as described above. Severe combined immune deficient (SCID) mice, which lack functional B and T lymphocytes were used to study B-cell malignancies. Cells from the Ramos B tumor cell line were injected intravenously. The mice were treated either with 19.6 mg/kg of cytotox in-conjugate anti-CD 19 antibody or 30 mg/kg naked anti-CD 19 antibody. An isotype control antibody or formulation buffer was used as a negative control. The isotype control was conjugated to the free toxin released by cleavage of the linker in Nl. The animals were dosed by intraperitoneal injection with approximately 200 μl of PBS containing antibody or vehicle. The antibody-cytotoxin conjugate was injected as a single dose on day 7, while the naked antibody was either injected as a single dose prophylactic model on day 1 or as a treatment model on days 7, 14 and 21. The mice were monitored daily for hind leg paralysis for approximately 6 weeks. Using an electronic caliper, the tumors were measured three dimensionally (height x width x length) and tumor volume was calculated. Mice were euthanized when there was hindleg paralysis.
As documented by Kaplan-Meier analysis (Figure 28), there was an increase in mean survival time upon treatment with cytotoxin-conjugated anti-CD 19 antibodies, naked anti-CD 19 antibodies administered prophylactically or anti-CD 19 antibodies administered as a treatment regimen. The largest increase in mean survival time shown was by prophylactic treatment using the naked anti-CD 19 antibody.
The change in body weight was also measured and calculated as percent change in weight. The data is shown in Figures 29A and B. Over a 30 day period, there was a net increase change in body weight with one cytotoxin-conjugate antibody and a net decrease change in body weight with antibody and cytotoxin (not conjugate). There was a net increase change in body weight with either the prophylactic naked anti-CD 19 antibody or the anti-CD 19 antibody treatment regimen.
Example 9: Treatment of in vivo Tumor Xenograft Model Using Naked Anti- CDl 9 Antibodies
Mice implanted with a lymphoma tumor were treated in vivo with naked anti- CD^ antibodies to examine the in vivo effect of the antibodies on tumor growth.
ARH-77 (human B lymphoblast leukemia; ATCC Accession No. CRL- 1621) and Raji (human B lymphocyte Burkitt's lymphoma; ATCC Accession No. CCL-86) cells were expanded in vitro using standard laboratory procedures. Male CB 17. SCID mice (Taconic, Hudson, NY) between 6-8 weeks of age were implanted subcutaneously in the right flank with 5 xlO6 ARH-77 or Raji cells in 0.2 ml of PBS/Matrigel (1: 1) per mouse. Mice were weighed and measured for tumors three dimensionally using an electronic caliper twice weekly after implantation. Tumor volumes were calculated as height x width x length/2. Mice with ARH-77 tumors averaging 80 mm3 or Raji tumors averaging 170 mm3 were randomized into treatment groups. The mice were dosed intraperitoneal Iy with PBS vehicle, isotype control antibody or naked anti-CD 19 HuMAb 2H5 on Day 0. Mice were euthanized when the tumors reached tumor end point (2000 mm3). The results are shown in Figure 30A (ARH-77 tumors) and 30B (Raji tumors). The naked anti-CD 19 antibody 21 D4 extended the mean time to reaching the tumor end point volume (2000 mm3) and slowed tumor growth progression. Thus, treatment with an anti-CD 19 antibody alone has a direct in vivo inhibitory effect on tumor growth.
Example 10: Production of Nonfucosylated HuMAbs
Antibodies with reduced amounts of fucosyl residues have been demonstrated to increase the ADCC ability of the antibody. In this example, the anti-CD 19 HuMAb 21D4 has been produced that is lacking in fucosyl residues.
The CHO cell line Ms704-PF, which lacks the fucosyltransferase gene, FUT 8 (Biowa, Inc., Princeton, NJ) was electroporated with a vector which expresses the heavy and light chains of antibody 21 D4. Drug-resistant clones were selected by growth in Ex- Cell 325-PF CHO media (JRH Biosciences, Lenexa, KS) with 6 mM L-glutamine and
500 μg/ml G418 (Invitrogen, Carlsbad, CA). Clones were screened for IgG expression by standard ELISA assay.
Oligosaccharide characterization of MAbs by CE-LIF
Comparative analysis of N-linked oligosaccharides derived from anti-CD 19 antibodies from a CHO fucosylating cell line and the Ms704-PF derived anti-CD 19 monoclonal antibody samples was done by capillary electrophoresis laser induced fluorescence (cLIF) (Chen and Evangelista (1998) Electrophoresis 15: 1892). The N- linked oligosaccharides of the purified antibody were released from IgG samples (100 μg) by overnight incubation of the samples with 12.5 mil PNGaseF (Prozyme) at 40°C. Under the conditions used, the N-linked glycans from the Fc portion of HuMAb 21 D4 expressed in CHO fucosylating and non-fucosylating cells were released. Following ethanol precipitation to remove MAb protein, the supernatant containing the glycans was dried by vacuum centrifugation and resuspended in 19mM 8-aminopyrene- 1,3,6- trisulfonate (APTS) (Beckman) under mild reductive amination conditions in which desialylation and loss of fucose residues was minimized (15% acetic acid and 1 M sodium cyanoborohydride in THF (Sigma)). The glycan labeling reaction was allowed to continue overnight at 40°C followed by 25-fold dilution of sample in water. APTS- labeled glycans were applied to capillary electrophoresis with laser induced fluorescence on a P/ACE MDQ CE system (Beckman) with reverse polarity, using a 50 μm internal diameter N-CHO coated capillary (Beckman) with 50 cm effective length. Samples were pressure (8 sec.) injected and separation was carried out at 20°C using Carbohydrate Separation Gel Buffer (Beckman) at 25 kV for 20 min. The separations were monitored using a laser-induced fluorescence detection system (Beckman) with a 3 mW argon ion laser and excitation wavelength of 488 nm and emission of 520 nm. (Ma and Nashabeh (1999) Anal. Chem. 71:5185). Differences in the oligosaccharide profile were observed between the antibody obtained from the fucosylating cell line as compared to the Ms704- PF cell line, consistent with an absence of fucose residues in the Ms704-PF derived anti- CD^ antibodies. Monosaccharide analysis by HPLC with HPAE-PAD
IgG samples (200 μg) were subjected to acid hydrolysis using either 2 N TFA (for estimating neutral sugars) or 6 N HCl (for estimating amino sugars) at 100°C for 4 h. Samples were dried by vacuum centrifugation at ambient temperature and were reconstituted in 200 μl water prior to analysis by HPAE-PAD (Dionex). Monosaccharides were separated using a CarboPac PAlO 4 x 250 mm column with pre- column Amino Trap and Borate Trap (Dionex). Procedures were followed according to Dionex Technical Note 53. Monosaccharide peak identity and relative abundance were determined using monosaccharide standards (Dionex).
The nonfucosylated anti-CD 19 21 D4 antibody was also tested using a standard capillary isoelectric focusing kit assay (Beckman Coulter). The assay returned observed pi values of pH 8.45 for fucosylated 21D4, 8.44 and 8.21 for fucosylated 21D4a, and 8.52 and 8.30 for the nonfucosylated 21D4 antibodies.
Example 11: Assessment of ADCC Activity of Anti-CD19 Antibody
In this example, fucosylated and nonfucosylated anti-CD 19 monoclonal antibodies were tested for the ability to kill CD 19+ cells in the presence of effector cells via antibody dependent cellular cytotoxicity (ADCC) in a fluorescence cytotoxicity assay.
Nonfucosylated human Anti-CD 19 monoclonal antibody 21 D4 was prepared as described above. Human effector cells were prepared from whole blood as follows. Human peripheral blood mononuclear cells were purified from heparinized whole blood by standard Ficoll-paque separation. The cells were resuspended in RPMI 1640 media containing 10% FBS (culture media) and 200 U/ml of human IL-2 and incubated overnight at 37°C. The following day, the cells were collected and washed once in culture media and resuspended at 2 x 107 cells/ml. Target CD 19+ cells were incubated with BATDA reagent (Perkin Elmer, Wellesley, MA) at 2.5 μl BATDA per 1 x 106 target cells/mL in culture media supplemented with 2.5mM probenecid (assay media) for 20 minutes at 37° C. The target cells were washed four times in PBS with 2OmM HEPES and 2.5mM probenecid, spun down and brought to a final volume of 1x10" cells/ml in assay media. The CD 19+ cell line ARH-77 (human B lymphoblast leukemia; ATCC Accession
No. CRL- 1621) was tested for antibody specific ADCC to the fucosylated and nonfucosylated human anti-CD 19 monoclonal antibody 21 D4 using the Delfia fluorescence emission analysis as follows. The target cell line ARH77 (100 μl of labeled target cells) was incubated with 50 μl of effector cells and 50 μl of either 21 D4 or nonfucosylated 21D4 antibody. A target to effector ratio of 1 :50 was used throughout the experiments. A human IgGl isotype control was used as a negative control. Following a 2100 rpm pulse spin and one hour incubation at 37° C, the supernatants were collected, quick spun again and 20 μl of supernatant was transferred to a flat bottom plate, to which 180 μl of Eu solution (Perkin Elmer, Wellesley, MA) was added and read in a Fusion Alpha TRF plate reader (Perkin Elmer). The % lysis was calculated as follows: (sample release - spontaneous release * 100) / (maximum release - spontaneous release), where the spontaneous release is the fluorescence from wells which only contain target cells and maximum release is the fluorescence from wells containing target cells and have been treated with 3% Lysol. Cell cytotoxicity % specific lysis for the ARH-77 cell line is shown in Figure 31. The CD 19+ expressing cell line ARH-77 showed antibody mediated cytotoxicity with the HuMAb anti-CD 19 antibody 21 D4 and an increased percentage of specific lysis associated with the nonfucosylated form of the anti-CD 19 antibody 21 D4. This data demonstrates that nonfucosylated HuMAb anti-CD 19 antibodies show increased specific cytotoxicity to CD 19+ expressing cells.
Example 12: Thermostability of Anti-CD19 Monoclonal Antibodies by Differential Scanning Calorimetry
The thermal stability of the anti-CD 19 monoclonal antibodies were compared using calorimetric analysis of their melting temperatures. Calorimetric measurements of melting temperatures ™ were carried out on a VP-
Capillary DSC differential scanning microcalorimeter platform that is combined with an autosampler (MicroCal LLC, Northampton, MA, USA). Sample cell volume is 0.144 mL. Denaturation data on the glycosylated and deglycosylated forms of the antibodies was obtained by heating the samples, at a concentration of 2.3μM, from 30 to 95 0C at a rate of 1 °C/min. The protein samples were present in phosphate-buffered saline (PBS) at pH 7.4. The same buffer was used in the reference cell to obtain the molar heat capacity by comparison. The observed thermograms were baseline corrected and normalized data analyzed based on a 2-state model, using the software Origin v7.0. The data is shown in Table 2. Table 2. Thermal stability measurement of anti-CD 19 antibodies
Clone Thermo Stability Tml (0C)
21D4 68.7
21D4a 69.7
5G7 68.5
5G7 IgG4 67.4
13Fl IgG4 68.4
46E8 66.4
47G4 67.2 Example 13: Assessment of GIycosylation Sites
The HuMAb anti-CD 19 antibody 5G7 was found to have an N-X-S/T glycosylation motif in the variable region by sequence analysis. The presence of an N- linked sequence site (N-X-S/T) is necessary but not sufficient for addition of carbohydrate to MAb. That is, it is possible to have an N-X-S/T sequence that does not actually add a carbohydrate due protein folding and solvent accessibility. Confirmation of a glycosylation site in the variable region of 5G7 was examined by both LC-MS and Western analysis.
Liquid Chromatography-Mass Spectrometry (LC-MS) is a standard tool for determining the mass of a protein, such as an antibody. Prior to analysis, the N-linked oligosaccharides of the anti-CD 19 HuMAbs 5G7 and 13Fl were released from IgG samples (100 μg) by overnight incubation of the samples with 12.5 mU PNGaseF (Prozyme) at 40°C. Under the conditions used, the N-linked glycans from the Fc portion of the HuMAbs were released. For clone 5G7, we observed two masses in high abundance; one (49,855 Da) corresponded to the predicted mass after PNGaseF digest to remove sugars in the constant region at the conserved N-linked site (N297), and a second mass (52,093 Da) consistent with addition of carbohydrate at a 2nd site. We have found that Fab-region glycans are not removed by PNGaseF digestion; therefore, this data supports the presence of carbohydrates in the variable region of clone 5G7. For clone 13Fl, the observed mass matched the predicted mass of the protein sequence without carbohydrates attached.
To confirm the above result, we completed a Western Blot assay on Fab fragments of clones 5G7 and 13Fl, with a carbohydrate-specific staining method. Fab and Fc fragments were produced by adding 1.25 μg of activated papain to 25 μg of IgG samples containing 1 mM cysteine. Samples were incubated at 40°C for 4 h and the reactions stopped with 30 mM iodoacetamide. Samples were analyzed by 4-20% Tris- Glycine SDS-PAGE followed by electro-blotting onto PVDF membrane. The carbohydrate specific staining of the blot was carried out using the Gel Code Glycoprotein Staining Kit (Pierce) following the protocol suggested by the manufacturer. The results detected Fab glycosylation in the 5G7 antibody, but not in the 13Fl antibody. These results showed that the 5G7 antibody is glycosylated in the Fab region. As discussed above, the anti-CD 19 monoclonal antibody 5G7 contains a variable region having a glycosylation site. Since glycosylation sites in the variable region may lead to increased immunogenicity of the antibody or altered pK values due to altered antigen binding, it may be advantageous to mutate the variable region N-X-S/T glycosylation motif sequence to reduce glycosylation. Using standard molecular biology techniques, the 5G7 antibody sequence was modified to change the N-I-S sequence starting at position 19 to either K-I-S (5G7-N19K) or Q-I-S (5G7-N19Q).
Example 14: Stability of Anti-CD19 Monoclonal Antibodies by Fluorescence Spectroscopy The stability of anti-CD 19 monoclonal antibodies were compared by measuring the midpoint of chemical denaturation by fluorescence spectroscopy.
Fluorescence measurements of chemical denaturation were performed on a SPEX Fluorolog 3.22 with a Micromax plate reader (SPEX, Edison, NJ). The measurements were performed on antibody samples that had been left for 24 hours to equilibrate in 16 different concentrations of guanidinium hydrochloride in PBS buffer. The measurements were made in black, low volume, non-binding surface 384-well plates (Corning, Acton, MA) and required 2 μM of antibody in a well volume of 12 μL. Fluorescence was excited at 280 nm and the emission spectra were measured between 300 and 400 nm. The scan speed was 1 second per nm and slits were set to 5nm bandpass. A buffer blank was performed using PBS and automatically subtracted from the data. The data is shown in Table 3.
Table 3. Fluorescence stability of anti-CD 19 antibodies r. Unfolding Midpoint Aggregation Peak
(M) (M)
21D4 3.01
21D4a 2.97
5G7 2.91
5G7 IgG4 2.63
27F3 2.77
13Fl IgG4 2.58 2.29
46E8 2.43 2.16
47G4 1.68 Example 15: Treatment of in vivo B cell Raji Tumors Using Anti-CD19 Antibodies
In this Example, SCID mice implanted with cancerous B cell tumors are treated in vivo with either naked anti-CD 19 antibodies or cytotoxin-conjugated anti-CD 19 antibodies to examine the in vivo effect of the antibodies on tumor growth. Cytotoxin-conjugated anti-CD 19 antibody 21 D4 was prepared as described above. Anti-CD 19-Nl conjugates and anti-CD 19-N2 conjugates were both tested in this example. Severe combined immune deficient (SCID) mice, which lack functional B and T lymphocytes were used to study B-cell malignancies. Cells from the Raji B tumor cell line were injected subcutaneously. The mice were treated with 30 mg/kg antibody or 0.3 μmole/kg (cytotoxin) antibody-cytotoxin conjugate. An isotype control antibody or formulation buffer was used as a negative control. The animals were dosed by intraperitoneal injection with approximately 200 μil of PBS containing antibody or vehicle. The antibody was either injected as a single dose (SD) on day 0 or as a repeat dose (RD) on days 0, 7 and 14. The mice were monitored daily for tumor growth using an electronic caliper; the tumors were measured three dimensionally (height x width x length/2) and tumor volume was calculated. Mice were euthanized when the tumors reached tumor end point (2000 mm3) or show greater than 20% weight loss. The results are shown in Figure 32. In each case, the anti-CD 19 antibody exhibiting smaller tumor volumes in comparison to the negative controls, with the cyto toxin-conjugate antibodies showing smaller tumor volumes than treatment with naked antibody.
The change in body weight was also measured and calculated as percent change in weight. The results are shown in Figure 33. The results showed a net decrease change in body weight with the cytotox in-conjugate antibodies and net increase in weight with either vehicle or naked antibodies.
Example 16: B cell Studies in Cynomolgus Monkeys
In this example, cynomolgus monkeys were injected intravenously with either parental anti-CD 19 antibody 21 D4 or nonfucosylated (nf) anti-CD 19 antibody 21 D4. Absolute leukocyte counts and leukocyte subsets were determined following dosing and compared to pre-dose values. Blood samples taken from cynomolgus monkeys were stained with either parental CD 19 antibody or nf anti-CD 19 antibody and analyzed by FACS using standard methods. B-cells from all monkeys included in the study stained positive with both parental and nf anti-CD 19 antibodies. Two males and two female monkeys were included in each group. Blood samples were taken at day -7 and pre-dosing. A slow bolus intravenous injection in a saphenous vein was performed and the animals were dosed with 1 mg/kg of parental or nf anti-CD 19 antibody. Blood samples were taken 24 hrs, 48 hrs, 72 hrs, and days 7, 14, 21 and 28 post dosing. Blood samples were taken for PK determination, hematology "and for flow cytometry. At each time point, the following cell surface antigens were monitored from blood; CD2+/CD20+ (all lymphocytes), CD20+ (B-lymphocytes), CD3+ (T-lymphocytes), CD3+/CD4+ (T-helper lymphocytes), CD3+/CD8+ (T-cytotoxic lymphocytes), CD3-/CD16+ (NK cells), CD3-/CD14+ (monocytes).
Figure 34 shows the change in the number of CD20 positive cells when compared to the average day -7 and pre-dose values. While parental anti-CD 19 antibody induced a 55% decrease in the number of CD20 positive B-cells after 24 hours, the non fucosylated antibody produced a more profound inhibition dropping the B-cell counts by approximately 90%. In the nf anti-CD 19 group, the B-cell counts remained at this level at days, 2, 3 and day 7 post treatment while the parental antibody appears to begin to return back to baseline. Figure 35 shows the % change from baseline for CD20 positive cells for each of the individual monkeys. All four monkeys treated with nf anti-CD 19 antibody showed a more significant drop in the % of CD20 positive cells when compared to parental anti-CD 19 antibody. Together these data imply that the nf anti-CD 19 antibody is more efficacious at depleting circulating B-cells when compared to the parental antibody.
Example 17: Immunohistochemistry Studies of Anti-CD19 Antibodies
To assess the tissue binding profiles of HuMab anti-CD 19, FITC conjugated 21D4 (21D4-FITC, F:P = 4) and nonfucosylated 21D4 (n£21D4) (nf21D4-FITC, F:P = 3) were examined in a panel of normal (non-neoplastic) human tissues, including spleen, tonsil, small intestine, cerebellum, cerebrum, heart, liver, lung, and kidney (1-2 sample/each), as well as B cell neoplasms, including chronic lymphocytic leukemia, follicular lymphoma, marginal zone lymphoma, mantle cell lymphoma, and diffuse large B cell lymphoma (1-2 sample/each). Nonfucosylated 21D4 antibodies were prepared as described above. FITC conjugated Hu-IgGi (Hu-IgGi-FITC) was used as isotype control antibody. Snap frozen and OCT embedded normal and lymphoma tissues were purchased from Cooperative Human Tissue Network (Philadelphia, PA) or National Disease Research Institute (Philadelphia, PA). Cryostat sections at 5 μm were fixed with acetone for 10 min at room temperature, and stored at -80 0C until use. Indirect peroxidase immunostaining using EnVision+System (Dako. Carpinteria, CA) was performed following our routine protocol. Briefly, slides were washed with PBS (Sigma, St. Louis, MO) twice, and then incubated with peroxidase block supplied in Dako EnVision+System for 10 minutes. After two washes with PBS, slides were incubated with Dako protein block supplemented with 1% human gamma globulins and 1 mg/ml of heat aggregated human IgG for 20 min to block the non-specific binding sites. Subsequently, primary antibodies (21 D4-FITC and nf21 D4-FITC at 0.4, or 2 μg/ml) or isotype control (Hu-IgGl-FITC at 0.4 or 2 μg/ml), were applied onto sections and incubated for 1 hr. Following three washes with PBS, slides were incubated with mouse anti-FITC antibody (20 μg/ml) for 30 min. After another three washes with PBS, the slides were incubated with the peroxidase-conjugated anti-mouse IgG polymer supplied in the Dako EnVision+System for 30 min. Finally, slides were washed as above and reacted with DAB substrate-chromogen solution supplied in the Dako EnVision+System for 6 min. Slides were then washed with deionized water, counterstained with Mayer's hematoxylin (Dako), dehydrated, cleared and coverslipped with Permount (Fischer Scientific, Fair Lawn, NJ) following routine histological procedure. Specific staining with both 21 D4-FITC and nf21 D4-FITC was observed in lymphoid or lymphoid-rich tissues (spleen, tonsil and small intestine) and lymphoma tissues. In spleen and tonsil, strong specific staining was primarily distributed in the B cell regions, i.e. lymphatic nodules of the spleen, mantle zone and germinal center of the tonsil. In small intestine, strong specific immunoreactivity was mainly localized in Peyer's patch or lymphoid aggregates, as well as weak to strong staining in diffuse lymphocytes in lamina propria of the mucosa. Strong staining was also displayed in tumor cells of follicular lymphoma and marginal zone lymphoma, as well as moderate to strong staining in chronic lymphocytic leukemia, diffuse large B cell lymphoma, and mantle cell lymphoma.
In normal cerebellum, cerebrum, heart, liver, lung, and kidney tissues, no meaningful staining was observed when stained with either 21D4-FITC or nf21D4-FITC except some staining in focal lymphoid cells or aggregates in lung and kidney tissues. In addition, these tissues were stained at higher concentrations up to lOμg/ml. Similarly, no specific staining was observed as compared with isotype control antibody.
Comparisons of 21D4-FITC and nf2ID4-FITC displayed similar staining patterns in all tissues. The specific staining was saturated or close to saturated at 0.4 μg/ml.
However, the staining intensity by 21D4-FITC is about 0.5-1 grade stronger than that by nf21D4-FITC. This maybe partially due to higher F:P ratio of 21D4-FITC (4 vs. 3).
Example 18: Assessment of Cell Killing of an Anti-CD19 Antibody
In this example, anti-CD 19 monoclonal antibodies alone or conjugated to a cytotoxin were tested for the ability to kill CD 19+ cell lines in a thymidine incorporation assay.
Anti-CD 19 monoclonal antibody was conjugated to a cytotoxin (Nl) via a linker, such as a peptidyl, hydrazone or disulfide linker. The CD 19+ expressing Raji or SU- DHL-6 cell lines were seeded at IxIO4 cells/well. Either anti-CD 19 antibody alone or an anti-CD 19 antibody-cytotoxin conjugate was added to the wells at a starting concentration of 30 nM and titrated down at 1 :3 serial dilutions for 8 dilutions. An isotype control antibody that is non-specific for CD 19 was used as a negative control. Plates were allowed to incubate for 69 hours. The plates were then pulsed with 0.5 μCi of 3H-thymidine for 24 hours, harvested and read in a Top Count Scintillation Counter (Packard Instruments, Meriden, CT). The results are shown in Figure 36 along with the EC50 values. Figure 36A shows naked antibody on Raji cells. Figure 36B shows naked antibody on SU-DHL-6 cells. Figure 36C shows cytotoxin-conjugated anti-CD 19 antibody on SU-DHL-6 cells. This data demonstrates that the anti-CD 19 antibody 21 D4 binds to and kills Raji B-cell tumor cells and has an unexpectedly high level of cell killing on SU-DHL-6 cells. Example 19: B-cell Depletion Studies
To determine if the anti-CD19 antibodies were capable of depleting B-cells, a whole blood B-cell depletion assay was set up.
Human whole blood was purchased from AllCells Inc. (Berkeley, CA) and delivered the same day at room temperature. Two ml of whole blood was incubated in the absence or presence of l-30mg/ml of the indicated antibodies, or PBS as the untreated group. The blood-antibody mixture was incubated overnight at 37°C with 5% CO2. On the day of the experiment, the blood was lysed twice with RBC lysis buffer at 1:10 ratio by incubating for 10 minutes followed by centrifugation. After the second spin, the cell pellets were washed once with FACS buffer (PBS plus Calcium and Magnesium with 2% FBS and 20% versene), and followed by FACS staining with anti-CD3 antibody (Becton Dickinson) as the T-CeIl makers and anti-CD22 antibody (Becton Dickinson) as a B-cell makers using standard Flow cytometry protocols. Cells were incubated on ice for 20 min prior to the final washes and resuspended in 5 mg/ml propidium iodide solution (Sigma) in FACS buffer. Data was collected by flow cytometry using a FASCalibur system and CellQuest software by Becton Dickinson, and analyzed via FlowJo software using lymphocyte size gating. Per cent change was calculated by determining the % positive B- cells in the non-treated group minus the % positive B-cells in the antibody treated group divided by % positive B-cells in the non treated group times 100. The results are shown in Table 4. From a healthy blood donor, 8.7% B-cells remained in the blood following an overnight incubation (no antibody). Incubating whole blood with 30 mg/ml of positive control Rituxan led to a 46% depletion in the number of B-cells when compared to the untreated, no antibody group. The group treated with non-fucosylated (nf) anti-CD 19 antibody 21 D4 had a pronounced effect on B-cell depletion, inhibiting B-cells by -40%. Parental antibody 21 D4 had a modest effect on B-cell counts.
Table 4. B-cell depletion from whole blood
Sample % Positive (CD22) % Change
No antibody 8.7
Isotype control 7.5 14.2
Rituxan® 4.7 46.3
Parental anti-CD19 mAb 7.0 20.0
Nf anti-CD19 mAb 5.2 40.5 Example 20: In Vivo Efficacy of Anti-CD19 Immunoconjugate in Subcutaneous Xenograft Model
To determine if the anti-CD 19 immunoconjugate was capable of inhibiting or reducing tumor growth in vivo, a subcutaneous xenograft model in SCID mice was tested. SCID mice were implanted subcutaneously with 1 x 107 Raji cells in 0.1 ml PBS and 0.1 ml matrigel per mouse. Tumor formation was monitored until the mean tumor volume was measured (using precision calipers) to be about 50 mmJ. Groups of eight tumor- bearing mice were treated with a single dose of one of: (a) a vehicle control, (b) isotype control, (c) anti-CD 19 antibody 21D4, or (d) immunoconjugate anti-CD 19-N2, using antibody 21 D4. Immunoconjugate CD19-N2 and isotype control-N2 (IgG-N2) were administered to the mice intraperitoneally (i.p.) at a dose of 0.3 μmol/kg of N2 equivalents. Anti-CD 19 antibody was administered at 25.7 mg/kg (i.e., the equivalent protein dose to the N2 equivalents used for the immunoconjugate CD19-N2). Tumor growth was monitored by measurement with precision calipers over the course of the experiment. As is evident in Figure 37, a single dose treatment with immunoconjugate CD 19-N2 resulted in tumor- free mice within 10 days (and remained tumor- free up to 60 days) as compared to the mice having tumors growing in size when treated with the controls or anti-CD 19 antibody alone.
Example 21: In Vivo Efficacy of Anti-CD19 Immunoconjugate in Burkitt's Lymphoma Model
To determine if the anti-CD 19 immunoconjugate was capable of inhibiting or reducing tumor growth in vivo in a dose-dependent manner, a subcutaneous Burkitt's lymphoma SCID mouse model was tested. SCID mice were implanted subcutaneously with 1 x 107 Raji cells in 0.1 ml PBS and 0.1 ml matrigel per mouse. Tumor formation was monitored until the mean tumor volume was measured (using precision calipers) to be about 70 mm3. Groups of eight tumor-bearing mice were treated with one of: (a) a vehicle control, (b) anti-CD 19 antibody 21 D4, or (c) immunoconjugate anti-CD 19-N2, using antibody 21 D4. Two doses of immunoconjugate CD 19-N2 were administered to each group of mice i.p., a week apart, at one of the following doses: 0.3 μmol/kg, 0.1 μmol/kg, 0.03 μmol/kg and 0.01 μmol/kg of N2 equivalents. Anti-CD 19 antibody was administered at 25 mg/kg (i.e., the equivalent protein dose to the N2 equivalents used for the immunoconjugate CD19-N2). Tumor growth was monitored by measurement with precision calipers over the course of the experiment. As is evident in Figure 38, tumor volume is reduced in a dose-dependent manner, with immunoconjugate CD19-N2 at 0.3 μmol/kg resulting in tumor-free mice by day 20-30 as compared to the lower doses in which the tumors increased in volume.
Example 22: In Vivo Efficacy of Anti-CD19 Immunoconjugate in Systemic Model
To determine if the anti-CD 19 immunoconjugate was capable of inhibiting or reducing tumor growth in vivo in a dose-dependent manner, a subcutaneous Burkitt's lymphoma SCID mouse model was tested.
SCID mice were implanted intravenously through a tail veil with 1 x 106 Raji cells in 0.1 ml PBS per mouse. One week post-implantation, groups of six mice were treated with a single dose of one of: (a) a vehicle control, (b) anti-CD 19 antibody 21 D4, or (c) immunoconjugate anti-CD 19-N2, using antibody 21 D4. Immunoconjugate CD 19- N2 was administered to each group of mice i.p. at one of the following doses: 0.3 μmol/kg or 0.1 μmol/kg of N2 equivalents. Anti-CD19 antibody was administered at 30 mg/kg (i.e., the equivalent protein dose to the N2 equivalents used for the immunoconjugate CD19-N2). Tumor growth was monitored over the course of the experiment by measuring the development of hind-leg paralysis as a result of infiltration of Raji cells into central nervous system. As is evident in Figure 39, none of the mice developed hind leg paralysis when treated with immunoconjugate CD19-N2 at 0.3 μmol/kg, while 15% of the mice did not develop hind leg paralysis when treated with immunoconjugate CD19-N2 at 0.1 μmol/kg. In contrast, all the mice treated with anti- CD 19 alone developed hind leg paralysis within 50 days of implantation.
Example 23: Single Dose Pharmacology in Cynomolgus Monkeys
To assess the pharmacology of anti-CD 19 antibody 21 D4, single i.v. injections of 0.01, 0.1, 1, or 10 mg/kg of the non-fucosylated (NF) antibody were administered to cynomolgus monkeys. CD20+ B cells were assessed by FACS. A 100-μL aliquot of each blood sample was placed into a clean, labeled tube, and an appropriate quantity of a commercially available fluorochrome labeled anti-CD20 antibody was added. The aliquot was incubated for approximately 30 minutes at room temperature and protected from light. After labeling, a commercially available lysing solution was added to remove red blood cells, and the remaining intact cells (approximately 1 to 2 x 106 cells/mL) were analyzed immediately or stored at approximately 40C until analysis (no longer than 120 hours after blood collection). The suspension was slowly wanned to room temperature immediately prior to analysis. B cells (CD20+) were decreased in a dose-dependent manner after administration of 21D4 (Figure 40A) with minimal or no depletion at 0.01 mg/kg. B cells decreased to 16% to 32% of baseline after administration of 0.1 mg/kg. Recovery of B cells was seen at 56 days after the administration. In this study, the magnitude and length of B-cell depletion was similar to that of a 0.1 mg/kg injection of rituximab (Figure 40B). B cells at nadir were 3% to 9% of baseline after the administration of 1 mg/kg of
21D4. In 2 of 4 animals, B cells started to recover at 36 days after the administration and were fully recovered within 7 months of the administration. In the other 2 animals, B-cell recoveries began 6 to 1 1 weeks after the administration and were 56% and 58% of baseline at 7 months after administration. The decrease in B cells after the administration of 10 mg/kg of 21D4 was similar to that after 1 mg/kg (3% to 1 1% vs 3% to 9%). In this study, a necropsy was performed on 4 animals at 15 days after the administration. Test article associated findings were limited to mild splenic lymphoid follicular atrophy in 2 of the 4 animals. This was characterized by the near absence of recognizable germinal centers and a reduction in the size of the lymphoid follicles, the primary locations for B cells within the spleen.
Lymphoid follicles in other tissues (mandibular and mesenteric lymph nodes and gut- associated lymphoid tissues) were not similarly affected. Two additional animals were held for a recovery period and B cells recovered to > 75% of baseline within 20 weeks after the administration. A necropsy was performed on these animals on Day 184 and there were no pharmacological effects in the spleen or lymph node seen upon microscopic pathology evaluation. Thus, administration of NF 21 D4 to cynomolgus monkeys was well-tolerated and resulted in the expected pharmacological effect of depletion of CD 19+ B cells.
Example 24: Multiple Dose Pharmacology in Cynomolgus Monkeys
The monthly administration of 10 mg/kg (x3) of non-fucosylated (NF) 21D4 resulted in B-cell counts of < 5% of baseline through Day 85 in 4 of the 6 animals. In the other 2 animals, B cells were < 10% of baseline after the first dose. In 1 of these animals, B cells increased to 17% of baseline on Day 29, and then remained stable through Day 85. In the other animal, B-cell counts increased to 69% of baseline by Day 71. IgG and IgM levels were measured throughout the study and were not affected by the administration of NF 21D4. A necropsy was performed on 4 of the 6 animals on Day 92. Histologic findings consisted of mild to moderate lymphoid follicular atrophy in the spleen (2 of 4 animals) and mesenteric lymph node (1 of 4 animals). This was characterized by the near absence of recognizable germinal centers and a reduction in the size of the lymphoid follicles, the primary locations for B cells within the spleen. The remaining 2 animals were held for a recovery period. B-cell counts started to increase on Day 169 and were at 31 and 38% of baseline on Day 225. These animals will be monitored until B-cell counts are > 75% of the baseline value. Weekly administration of 1, 10 or 50 mg/kg also resulted in significantly decreased B cells counts; < 16% of baseline at nadir. A necropsy was performed on 6 per group on Day 30 and histologic findings included minimal to moderate diffuse atrophy of lymphoid follicles in the spleen in 10 of 18 animals. Reductions in CD20+ lymphocytes in the spleen, lymph nodes, and bone maiτow were also seen in the majority of the animals.
Example 25: Tumor growth inhibition in vivo by anti-CD19-cytotoxin A
This example demonstrates the utility of anti-CD 19-cytotoxin A conjugate as a targeted therapeutic against lymphoma using three human lymphoma models (Raji and Daudi in SCID mice and Ramos in Esle nude mice). The structure ot cytotoxin A is shown in Figure 46.
These animal models were used to test the efficacy of the anti-CD 19-cytotoxin A conjugate in vivo. A cytotoxin conjugate of the CD 19 antibody 21 D4 is referred to herein as CD 19-cytotoxin A, which is comprised of the CD 19 antibody 21 D4 linked to cytotoxin A. Cytotoxin A and preparation thereof, is described further in U.S. Application Serial No. 60/882,461, filed on December 28, 2006, and U.S. Application Serial No. 60/991,300, filed on November 30, 2007, the entire contents of which are specifically incorporated herein by reference. The cytotoxin A cytotoxin is in prodrug form, and requires not only release from the antibody for activity but also cleavage of a 4' carbamate group to release the active moiety.
To demonstrate the activity of anti-CD 19-cytotoxin A in Raji lymphoma model, a therapy study was carried out in SCID mice bearing subcutaneous Raji xenografts. Raji cells (10 million in 0.1 ml PBS and 0.1 ml Matrigel™/ mouse) were implanted subcutaneously into SCID mice, and when tumors reached an average size of 190 mm3, groups of 8 mice were treated by ip injection of a single dose of either anti-CD 19- cytotoxin A at 0.03, 0.1 or 0.3μmol/kg body weight. In addition, control group were injected with vehicle alone, or isotype control antibody cytotoxin A conjugate at 0.1 or 0.3 μmol/kg body weight. Tumor volumes (LWH/2) and weights of mice were recorded throughout the course of the study, which was allowed to proceed for 63 days post dosing. Results are shown in Figures 41 and 42. Figure 41 depicts the results in a single graph and Figure 42 depicts the results including isotype controls. The results demonstrate that the anti-CD 19-cytotoxin A conjugate is efficacious in the treatment of lymphoma, and that therapy is dose-dependent. A second lymphoma model was earned out using Ramos xenografts.
Ramos cells (10 million in 0.1 ml PBS and 0.1 ml Matrigel™/ mouse) were implanted subcutaneously into Esle nude mice (Jackson Laboratory), and when tumors reached an average size of 110 mm3, groups of 10 mice were treated by ip injection of either a single dose (day 0) or repeat doses (day 0, 11 and 25) of anti-CD 19-cytotoxin A at 0.3μmol/kg body weight. In addition, control groups were injected with vehicle alone, anti-CD 19 antibody alone, or isotype control antibody cytotoxin A conjugate at 0.3 μmol/kg body weight. Tumor volumes (LWH/2) and weights of mice were recorded throughout the course of the study, which was allowed to proceed for 60 days post dosing. Results are shown in Figure 43. The results demonstrate that the anti-CD 19-cytotoxin A conjugate is also efficacious in the treatment of lymphoma in this animal model. A third lymphoma model was carried out using Daudi xenografts. Daudi cells (10 million in 0.1 ml PBS and 0.1 ml Matrigel™/ mouse) were implanted subcutaneously into SCID mice, and when tumors reached an average size of 70 mm3, groups of 8 mice were treated by ip injection of a single dose of either anti-CD 19-cytotoxin A at 0.1 or 0.3μmol/kg body weight. In addition, control groups were injected with vehicle alone, anti-CD 19 antibody alone matching 0.3 μmol/kg anti-CD 19-cytotoxin A dose, or isotype control antibody cytotoxin A conjugate at 0.1 or 0.3 μmol/kg body weight. Tumor volumes (LWH/2) and weights of mice were recorded throughout the course of the study, which was allowed to proceed for 58 days post dosing. Results are shown in Figure 44. The results demonstrate that the anti-CD 19-cytotoxin A conjugate is also efficacious in the treatment of lymphoma in this animal model.
Example 26: Tumor growth inhibition in vivo by anti-CD19-N2
This example demonstrates the efficacy of anti-CD 19-N2 in the SU-DHL-6 lymphoma model. A cytotoxin conjugate of the CD 19 antibody 21 D4 is referred to herein as CD19-N2, which is comprised of the CD 19 antibody 21D4 linked to N2.
A therapy study was carried out in SCID mice bearing subcutaneous SU-DHL-6 xenografts. SU-DHL-6 cells (10 million in 0.1 ml PBS and 0.1 ml Matrigel™/ mouse) were implanted subcutaneously into SCID mice, and when tumors reached an average size of 140 mm3, groups of 10 mice were treated by ip injection of a single dose of either anti-CD 19-N2 at 0.1 or 0.3 μmol/kg body weight. In addition, control group were injected with vehicle alone, or isotype control antibody N2 conjugate at 0.1 or 0.3 μmol/kg body weight. Tumor volumes (LWH/2) and weights of mice were recorded throughout the course of the study, which was allowed to proceed for 64 days post dosing. Results are shown in Figure 45. The results demonstrate that the anti-CD 19-N2 conjugate is efficacious and selective in the treatment of lymphoma, and that therapy is dose-dependent. SUMMARY OF SEQUENCE LISTING
Figure imgf000202_0001
Figure imgf000203_0001
Figure imgf000204_0001

Claims

CLAIMSWhat is claimed is:
1. An antibody-partner molecule conjugate comprising an isolated human monoclonal antibody, or an antigen-binding portion thereof, wherein the antibody binds human CD 19 and exhibits at least one of the following properties:
(a) binds to human CD 19 with a K0 of Ix 1(T7 M or less;
(b) binds to Raji and/or Daudi B-cell tumor cells;
(c) is internalized by CD19-expressing cells; (d) exhibits antibody dependent cellular cytotoxicity (ADCC) against CD 19 expressing cells; and
(e) inhibits growth of CD19-expressing cells in vivo when conjugated to a cytotoxin, and a partner molecule, wherein the partner molecule is a therapeutic agent.
2. The antibody-partner molecule of claim 1, wherein the antibody exhibits at least two of properties (a), (b), (c), (d), and (e).
3. The antibody-partner molecule of claim I, wherein the antibody exhibits at least three of properties (a), (b), (c), (d), and (e).
4. The antibody-partner molecule of claim 1, wherein the antibody exhibits at least four of properties (a), (b), (c), (d), and (e).
5. The antibody-partner molecule of claim 1, wherein the antibody exhibits all five of properties (a), (b), (c), (d), and (e).
6. The antibody-partner molecule of claim I, wherein the antibody binds to human CD 19 with a KD of 5 xlO"8 M or less.
7. The antibody-partner molecule of claim I , wherein the antibody binds to human CD 19 with a K0 of 5 xlθ"y M or less.
8. An antibody-partner molecule conjugate comprising an isolated monoclonal antibody, or antigen binding portion thereof, which binds an epitope on human CD 19 recognized by a reference antibody, wherein the reference antibody comprises:
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO:8;
(b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO:9;
(c) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:2 and a light chain variable region comprising the amino acid sequence of SEQ
ID NO: 10;
(d) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:3 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 1 1 ; (e) a heavy chain variable region comprising the amino acid sequence of SEQ
ID NO:4 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12;
(f) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:5 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13;
(g) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14; or
(h) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:7 a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15, and a partner molecule, wherein the partner molecule is a therapeutic agent.
9. The antibody-partner molecule conjugate of claim 8, wherein the reference antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8.
10. The antibody-partner molecule conjugate of claim 8, wherein the reference antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 9.
1 1. The antibody-partner molecule conjugate of claim 8, wherein the reference antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 2 and a light chain variable region comprising the amino acid sequence of SEQ ID
NO: 10.
12. The antibody-partner molecule conjugate of claim 8, wherein the reference antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 3 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 11.
13. The antibody-partner molecule conjugate of claim 8, wherein the reference antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12.
14. The antibody-partner molecule conjugate of claim 8, wherein the reference antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 5 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13.
15. The antibody-partner molecule conjugate of claim 8, wherein the reference antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14.
16. The antibody-partner molecule conjugate of claim 8, wherein the reference antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15.
17. An antibody-partner molecule conjugate comprising an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a heavy chain variable region that is the product of or derived from a human VH 5-51 gene, a human VH 5-51 gene, or a human VH 1 -69 gene, wherein the antibody specifically binds CD 19, and a partner molecule, wherein the partner molecule is a therapeutic agent.
18. An antibody-partner molecule conjugate comprising an isolated monoclonal antibody, or an antigen-binding portion thereof, comprising a light chain variable region that is the product of or derived from a human Vκ Ll 8 gene, a human VK A27 gene, or a human Vκ Ll 5 gene, wherein the antibody specifically binds to CD 19, and a partner molecule, wherein the partner molecule is a therapeutic agent.
19. The antibody-partner molecule conjugate of claim 1, wherein the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 16;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 23;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 30;
(d) a light chain variable region CDRl comprising SEQ ID NO: 37; (e) a light chain variable region CDR2 comprising SEQ ID NO: 44; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 51.
20. The antibody-partner molecule conjugate of claim 1, wherein the antibody comprises (a) a heavy chain variable region CDRl comprising SEQ ID NO: 16;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 23;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 30;
(d) a light chain variable region CDRl comprising SEQ ID NO: 37;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 44; and (f) a light chain variable region CDR3 comprising SEQ ID NO: 52.
21. The antibody-partner molecule conjugate of claim 1 , wherein the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 17; (b) a heavy chain variable region CDR2 comprising SEQ ID NO: 24;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 31 ;
(d) a light chain variable region CDRl comprising SEQ ID NO: 38;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 45; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 53.
22. The antibody-partner molecule conjugate of claim 1 , wherein the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 18;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 25; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 32;
(d) a light chain variable region CDRl comprising SEQ ID NO: 39;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 46; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 54.
23. The antibody-partner molecule conjugate of claim 1, wherein the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 19;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 26;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 33; (d) a light chain variable region CDRl comprising SEQ ID NO: 40;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 47; and (!) a light chain variable region CDR3 comprising SEQ ID NO: 55.
24. The antibody-partner molecule conjugate of claim 1 , wherein the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 20;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 27;
(c) a heavy chain variable region CDR3 comprising SEQ ID NO: 34;
(d) a light chain variable region CDRl comprising SEQ ID NO: 41; (e) a light chain variable region CDR2 comprising SEQ ID NO: 48; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 56.
25. The antibody-partner molecule conjugate of claim 1, wherein the antibody comprises: (a) a heavy chain variable region CDRl comprising SEQ ID NO: 21 ;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 28; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 35;
(d) a light chain variable region CDRl comprising SEQ ID NO: 42;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 49; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 57.
26. The antibody-partner molecule conjugate of claim 1, wherein the antibody comprises:
(a) a heavy chain variable region CDRl comprising SEQ ID NO: 22;
(b) a heavy chain variable region CDR2 comprising SEQ ID NO: 29; (c) a heavy chain variable region CDR3 comprising SEQ ID NO: 36;
(d) a light chain variable region CDRl comprising SEQ ID NO: 43;
(e) a light chain variable region CDR2 comprising SEQ ID NO: 50; and
(f) a light chain variable region CDR3 comprising SEQ ID NO: 58.
27. An antibody-partner molecule conjugate comprising an isolated monoclonal antibody, or antigen binding portion thereof, comprising:
(a) a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-7; and
(b) a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:8-l 5; wherein the antibody specifically binds a human CD 19 protein, and a partner molecule, wherein the partner molecule is a therapeutic agent.
28. The antibody-partner molecule of claim 27, wherein the antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8.
29. The antibody-partner molecule of claim 27, wherein the antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 9.
30. The antibody-partner molecule of claim 27, wherein the antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 2 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10.
31. The antibody-partner molecule of claim 27, wherein the antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 3 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 11.
32. The antibody-partner molecule of claim 27, wherein the antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12.
33. The antibody-partner molecule of claim 27, wherein the antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID
NO: 5 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 13.
34. The antibody-partner molecule of claim 27, wherein the antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14.
35. The antibody-partner molecule of claim 27, wherein the antibody comprises: a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 7 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15.
36. An antibody-partner molecule conjugate comprising an isolated monoclonal antibody, or an antigen binding portion thereof, which binds an epitope on the human CD70 protein recognized by an antibody comprising:
(a) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1 and a light chain variable region comprising the amino acid sequence of SEQ
ID NO:8;
(b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: I and a light chain variable region comprising the amino acid sequence of SEQ ID NO:9; (c) a heavy chain variable region comprising the amino acid sequence of SEQ
ID NO:2 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10;
(d) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:3 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: l l;
(e) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:4 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 12;
(f) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:5 and a light chain variable region comprising the amino acid sequence of SEQ
ID NO: 13; (g) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 14; or
(h) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:7 a light chain variable region comprising the amino acid sequence of SEQ ID NO: 15, and a partner molecule, wherein the partner molecule is a therapeutic agent.
37. A composition comprising the antibody-partner molecule conjugate of claim 1 and a pharmaceutically acceptable carrier.
38. The antibody-partner molecule conjugate of claim 1, wherein the therapeutic agent is a cytotoxin.
39. A composition comprising the antibody-partner molecule conjugate of claim 38 and a pharmaceutically acceptable carrier.
40. The antibody-partner molecule conjugate of claim 1, wherein the therapeutic agent is a radioactive isotope.
41. A composition comprising the antibody-partner molecule conjugate of claim 40 and a pharmaceutically acceptable carrier.
42. A method of inhibiting growth of a CD 19-expressing tumor cell comprising contacting the CDl 9-expressing tumor cell with the antibody-partner molecule conjugate of claim 1 such that growth of the CD 19-expressing tumor cell is inhibited.
43. A method of depleting B cells in a subject comprising administering to the subject the antibody-partner molecule conjugate of claim 1 in an amount effective to deplete B cells from the subject.
44. The method of claim 43, wherein said CD19-expressing tumor cell is a B- cell malignancy cell.
45. The method of claim 44, wherein said B-cell malignancy is non-
Hodgkin's lymphoma.
46. The method of claim 44, wherein said B-cell malignancy is mantle cell lymphoma.
47. A method of treating cancer in a subject comprising administering to the subject an antibody-partner molecule conjugate of claim 1 such that the cancer is treated in the subject.
48. The method of claim 47, wherein the cancer is non-Hodgkin's lymphoma.
49. The method of claim 47, wherein said cancer is mantle cell lymphoma.
50. The antibody-partner molecule conjugate of claim 1, wherein the partner molecule is conjugated to the antibody by a chemical linker.
51. The antibody-partner molecule conjugate of claim 50, wherein the chemical linker is selected from the group consisting of peptidyl linkers, hydrazine linkers, and disulfide linkers.
52. The antibody-partner molecule conjugate of claim 1, wherein antibody, or antigen binding portion thereof, is nonfucosylated.
PCT/US2007/087393 2006-12-13 2007-12-13 Human antibodies that bind cd19 and uses thereof WO2009054863A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
BRPI0718349-6A2A BRPI0718349A2 (en) 2006-12-13 2007-12-13 CONJUGATED ANTIBODY PARTNERSHIP COMPOSITION, METHOD OF INHIBITING THE GROWTH OF A TUMOR CELL EXPRESSING CD19, B-CELL EXHAUST METHOD IN A SUBJECT AND METHOD OF TREATMENT OF A SUBJECT TO A CANCER
EP07875235.9A EP2101817A4 (en) 2006-12-13 2007-12-13 Human antibodies that bind cd19 and uses thereof
MX2009006275A MX2009006275A (en) 2006-12-13 2007-12-13 Human antibodies that bind cd19 and uses thereof.
JP2009541583A JP5517626B2 (en) 2006-12-13 2007-12-13 Human antibodies that bind to CD19 and uses thereof
CN200780050552.1A CN101636502B (en) 2006-12-13 2007-12-13 Human antibodies that bind cd19 and uses thereof
CA002672800A CA2672800A1 (en) 2006-12-13 2007-12-13 Human antibodies that bind cd19 and uses thereof
US12/519,149 US20100104509A1 (en) 2006-12-13 2007-12-13 Human antibodies that bind cd19 and uses thereof
AU2007360636A AU2007360636A1 (en) 2006-12-13 2007-12-13 Human antibodies that bind CD19 and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US86990406P 2006-12-13 2006-12-13
US60/869,904 2006-12-13
US99170007P 2007-11-30 2007-11-30
US60/991,700 2007-11-30

Publications (2)

Publication Number Publication Date
WO2009054863A2 true WO2009054863A2 (en) 2009-04-30
WO2009054863A3 WO2009054863A3 (en) 2009-09-24

Family

ID=40580273

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/087393 WO2009054863A2 (en) 2006-12-13 2007-12-13 Human antibodies that bind cd19 and uses thereof

Country Status (13)

Country Link
US (1) US20100104509A1 (en)
EP (1) EP2101817A4 (en)
JP (1) JP5517626B2 (en)
KR (1) KR20090088940A (en)
CN (1) CN101636502B (en)
AR (1) AR064337A1 (en)
AU (1) AU2007360636A1 (en)
BR (1) BRPI0718349A2 (en)
CA (1) CA2672800A1 (en)
CL (1) CL2007003622A1 (en)
MX (1) MX2009006275A (en)
TW (1) TW200833713A (en)
WO (1) WO2009054863A2 (en)

Cited By (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2409712A1 (en) 2010-07-19 2012-01-25 International-Drug-Development-Biotech Anti-CD19 antibody having ADCC and CDC functions and improved glycosylation profile
EP2409993A1 (en) 2010-07-19 2012-01-25 International-Drug-Development-Biotech Anti-CD19 antibody having ADCC function with improved glycosylation profile
WO2014008218A1 (en) 2012-07-02 2014-01-09 Bristol-Myers Squibb Company Optimization of antibodies that bind lymphocyte activation gene-3 (lag-3), and uses thereof
EP2905030A1 (en) 2008-08-11 2015-08-12 E. R. Squibb & Sons, L.L.C. Human antibodies that bind lymphocyte activation gene-3 (LAG-3) and uses thereof
WO2015187835A2 (en) 2014-06-06 2015-12-10 Bristol-Myers Squibb Company Antibodies against glucocorticoid-induced tumor necrosis factor receptor (gitr) and uses thereof
WO2016033570A1 (en) * 2014-08-28 2016-03-03 Juno Therapeutics, Inc. Antibodies and chimeric antigen receptors specific for cd19
WO2016081748A2 (en) 2014-11-21 2016-05-26 Bristol-Myers Squibb Company Antibodies against cd73 and uses thereof
WO2017087678A2 (en) 2015-11-19 2017-05-26 Bristol-Myers Squibb Company Antibodies against glucocorticoid-induced tumor necrosis factor receptor (gitr) and uses thereof
WO2017152085A1 (en) 2016-03-04 2017-09-08 Bristol-Myers Squibb Company Combination therapy with anti-cd73 antibodies
WO2018083535A1 (en) * 2016-11-04 2018-05-11 Novimmune Sa Anti-cd19 antibodies and methods of use thereof
WO2018151821A1 (en) 2017-02-17 2018-08-23 Bristol-Myers Squibb Company Antibodies to alpha-synuclein and uses thereof
WO2018213297A1 (en) 2017-05-16 2018-11-22 Bristol-Myers Squibb Company Treatment of cancer with anti-gitr agonist antibodies
WO2018218056A1 (en) 2017-05-25 2018-11-29 Birstol-Myers Squibb Company Antibodies comprising modified heavy constant regions
WO2019243626A1 (en) 2018-06-22 2019-12-26 Genmab A/S Method for producing a controlled mixture of two or more different antibodies
WO2019215510A3 (en) * 2018-05-09 2020-01-09 Legochem Biosciences, Inc. Compositions and methods related to anti-cd19 antibody drug conjugates
WO2020154889A1 (en) * 2019-01-29 2020-08-06 上海鑫湾生物科技有限公司 Combination of antibody having fc mutant and effector cell, use thereof and preparation method therefor
EP3789399A1 (en) 2014-11-21 2021-03-10 Bristol-Myers Squibb Company Antibodies comprising modified heavy constant regions
WO2021067598A1 (en) 2019-10-04 2021-04-08 Ultragenyx Pharmaceutical Inc. Methods for improved therapeutic use of recombinant aav
US10980890B2 (en) 2014-05-28 2021-04-20 Legochem Biosciences, Inc. Compounds comprising self-immolative group
EP3684820A4 (en) * 2017-09-21 2021-05-26 Wuxi Biologics (Cayman) Inc. Novel anti-cd19 antibodies
US11059910B2 (en) 2012-12-03 2021-07-13 Novimmune Sa Anti-CD47 antibodies and methods of use thereof
US11167040B2 (en) 2015-11-25 2021-11-09 Legochem Biosciences, Inc. Conjugates comprising peptide groups and methods related thereto
US11173214B2 (en) 2015-11-25 2021-11-16 Legochem Biosciences, Inc. Antibody-drug conjugates comprising branched linkers and methods related thereto
CN113840841A (en) * 2019-05-20 2021-12-24 南京驯鹿医疗技术有限公司 Fully human antibody targeting CD19 and application thereof
US11236161B2 (en) 2014-06-02 2022-02-01 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Chimeric antigen receptors targeting CD-19
US11286300B2 (en) 2015-10-01 2022-03-29 Hoffmann-La Roche Inc. Humanized anti-human CD19 antibodies and methods of use
US11299551B2 (en) 2020-02-26 2022-04-12 Biograph 55, Inc. Composite binding molecules targeting immunosuppressive B cells
US11413353B2 (en) 2015-11-25 2022-08-16 Legochem Biosciences, Inc. Conjugates comprising self-immolative groups and methods related thereto
US11654197B2 (en) 2017-03-29 2023-05-23 Legochem Biosciences, Inc. Pyrrolobenzodiazepine dimer prodrug and ligand-linker conjugate compound of the same
US11707533B2 (en) 2019-09-04 2023-07-25 Legochem Biosciences, Inc. Antibody-drug conjugate comprising antibody against human ROR1 and use for the same
EP4249066A2 (en) 2014-12-23 2023-09-27 Bristol-Myers Squibb Company Antibodies to tigit
WO2023198744A1 (en) 2022-04-13 2023-10-19 Tessa Therapeutics Ltd. Therapeutic t cell product
US11793834B2 (en) 2018-12-12 2023-10-24 Kite Pharma, Inc. Chimeric antigen and T cell receptors and methods of use
RU2806333C2 (en) * 2018-05-09 2023-10-31 Легокем Байосайенсиз, Инк. Compositions and methods relating to drug conjugates with anti-cd19 antibodies
WO2023245106A1 (en) * 2022-06-16 2023-12-21 Abbvie Biotherapeutics Inc. Anti-cd19 antibody drug conjugates

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LT2211904T (en) 2007-10-19 2016-11-25 Seattle Genetics, Inc. Cd19 binding agents and uses thereof
US8852599B2 (en) 2011-05-26 2014-10-07 Bristol-Myers Squibb Company Immunoconjugates, compositions for making them, and methods of making and use
CA2876730A1 (en) 2012-07-13 2014-01-16 The Trustees Of The University Of Pennsylvania Enhancing activity of car t cells by co-introducing a bispecific antibody
CN105837689B (en) * 2015-01-13 2020-06-19 博生吉安科细胞技术有限公司 anti-CD19 monoclonal antibody and preparation method thereof
MA44909A (en) 2015-09-15 2018-07-25 Acerta Pharma Bv THERAPEUTIC ASSOCIATION OF A CD19 INHIBITOR AND A BTK INHIBITOR
CN106554414B (en) * 2015-09-18 2019-04-23 上海科济制药有限公司 Anti- CD19 human antibody and the immune effector cell for targeting CD19
WO2017075465A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by detecting and targeting gata3
WO2017075451A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by detecting and targeting pou2af1
HUE063911T2 (en) * 2016-06-02 2024-02-28 Bristol Myers Squibb Co Use of an anti-pd-1 antibody in combination with an anti-cd30 antibody in lymphoma treatment
WO2018049025A2 (en) 2016-09-07 2018-03-15 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses
WO2018183908A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
EP3606518A4 (en) 2017-04-01 2021-04-07 The Broad Institute, Inc. Methods and compositions for detecting and modulating an immunotherapy resistance gene signature in cancer
US20200071773A1 (en) 2017-04-12 2020-03-05 Massachusetts Eye And Ear Infirmary Tumor signature for metastasis, compositions of matter methods of use thereof
JOP20180042A1 (en) * 2017-04-24 2019-01-30 Kite Pharma Inc Humanized Antigen-Binding Domains and Methods of Use
WO2018232195A1 (en) 2017-06-14 2018-12-20 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
US11634488B2 (en) 2017-07-10 2023-04-25 International—Drug—Development—Biotech Treatment of B cell malignancies using afucosylated pro-apoptotic anti-CD19 antibodies in combination with anti CD20 antibodies or chemotherapeutics
WO2019014581A1 (en) 2017-07-14 2019-01-17 The Broad Institute, Inc. Methods and compositions for modulating cytotoxic lymphocyte activity
WO2019070755A1 (en) 2017-10-02 2019-04-11 The Broad Institute, Inc. Methods and compositions for detecting and modulating an immunotherapy resistance gene signature in cancer
EP3710039A4 (en) 2017-11-13 2021-08-04 The Broad Institute, Inc. Methods and compositions for treating cancer by targeting the clec2d-klrb1 pathway
US11994512B2 (en) 2018-01-04 2024-05-28 Massachusetts Institute Of Technology Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
US20210371932A1 (en) 2018-06-01 2021-12-02 Massachusetts Institute Of Technology Methods and compositions for detecting and modulating microenvironment gene signatures from the csf of metastasis patients
US12036240B2 (en) 2018-06-14 2024-07-16 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
CN111019905A (en) * 2018-09-12 2020-04-17 上海斯丹赛生物技术有限公司 CAR modified cell and application thereof in preparation of autoimmune disease drugs
CN112955172A (en) * 2018-09-17 2021-06-11 美国卫生和人力服务部 Bicistronic chimeric antigen receptor targeting CD19 and CD20 and uses thereof
US20210382068A1 (en) 2018-10-02 2021-12-09 Dana-Farber Cancer Institute, Inc. Hla single allele lines
WO2020081730A2 (en) 2018-10-16 2020-04-23 Massachusetts Institute Of Technology Methods and compositions for modulating microenvironment
US20220170097A1 (en) 2018-10-29 2022-06-02 The Broad Institute, Inc. Car t cell transcriptional atlas
WO2020131586A2 (en) 2018-12-17 2020-06-25 The Broad Institute, Inc. Methods for identifying neoantigens
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
US20220154282A1 (en) 2019-03-12 2022-05-19 The Broad Institute, Inc. Detection means, compositions and methods for modulating synovial sarcoma cells
US20220142948A1 (en) 2019-03-18 2022-05-12 The Broad Institute, Inc. Compositions and methods for modulating metabolic regulators of t cell pathogenicity
WO2020236967A1 (en) 2019-05-20 2020-11-26 The Broad Institute, Inc. Random crispr-cas deletion mutant
WO2020236792A1 (en) * 2019-05-21 2020-11-26 Novartis Ag Cd19 binding molecules and uses thereof
US20220282333A1 (en) 2019-08-13 2022-09-08 The General Hospital Corporation Methods for predicting outcomes of checkpoint inhibition and treatment thereof
US20220298501A1 (en) 2019-08-30 2022-09-22 The Broad Institute, Inc. Crispr-associated mu transposase systems
US11981922B2 (en) 2019-10-03 2024-05-14 Dana-Farber Cancer Institute, Inc. Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment
US11793787B2 (en) 2019-10-07 2023-10-24 The Broad Institute, Inc. Methods and compositions for enhancing anti-tumor immunity by targeting steroidogenesis
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
CN112679612B (en) * 2021-01-29 2022-07-01 武汉华美生物工程有限公司 anti-CD 19 humanized antibody and preparation method and application thereof
WO2022165419A1 (en) 2021-02-01 2022-08-04 Kyverna Therapeutics, Inc. Methods for increasing t-cell function
US20230210900A1 (en) 2022-01-04 2023-07-06 Kyverna Therapeutics, Inc. Methods for treating autoimmune diseases
WO2024077256A1 (en) 2022-10-07 2024-04-11 The General Hospital Corporation Methods and compositions for high-throughput discovery ofpeptide-mhc targeting binding proteins
WO2024124044A1 (en) 2022-12-07 2024-06-13 The Brigham And Women’S Hospital, Inc. Compositions and methods targeting sat1 for enhancing anti¬ tumor immunity during tumor progression
US20240285762A1 (en) 2023-02-28 2024-08-29 Juno Therapeutics, Inc. Cell therapy for treating systemic autoimmune diseases

Citations (165)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4391904A (en) 1979-12-26 1983-07-05 Syva Company Test strip kits in immunoassays and compositions therein
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4439196A (en) 1982-03-18 1984-03-27 Merck & Co., Inc. Osmotic drug delivery system
US4447224A (en) 1982-09-20 1984-05-08 Infusaid Corporation Variable flow implantable infusion apparatus
US4447233A (en) 1981-04-10 1984-05-08 Parker-Hannifin Corporation Medication infusion pump
US4475196A (en) 1981-03-06 1984-10-02 Zor Clair G Instrument for locating faults in aircraft passenger reading light and attendant call control system
US4486194A (en) 1983-06-08 1984-12-04 James Ferrara Therapeutic device for administering medicaments through the skin
US4487603A (en) 1982-11-26 1984-12-11 Cordis Corporation Implantable microinfusion pump system
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
EP0154316A2 (en) 1984-03-06 1985-09-11 Takeda Chemical Industries, Ltd. Chemically modified lymphokine and production thereof
US4596556A (en) 1985-03-25 1986-06-24 Bioject, Inc. Hypodermic injection apparatus
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
WO1987004462A1 (en) 1986-01-23 1987-07-30 Celltech Limited Recombinant dna sequences, vectors containing them and method for the use thereof
WO1988000052A1 (en) 1986-07-07 1988-01-14 Trustees Of Dartmouth College Monoclonal antibodies to fc receptor
US4790824A (en) 1987-06-19 1988-12-13 Bioject, Inc. Non-invasive hypodermic injection device
WO1989001036A1 (en) 1987-07-23 1989-02-09 Celltech Limited Recombinant dna expression vectors
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
EP0338841A1 (en) 1988-04-18 1989-10-25 Celltech Limited Recombinant DNA methods, vectors and host cells
US4881175A (en) 1986-09-02 1989-11-14 Genex Corporation Computer based system and method for determining and displaying possible chemical structures for converting double- or multiple-chain polypeptides to single-chain polypeptides
US4912227A (en) 1984-02-21 1990-03-27 The Upjohn Company 1,2,8,8A-tetrahydrocyclopropa(c)pyrrolo(3,2-e)-indol-4-(5H)-ones and related compounds
EP0368684A1 (en) 1988-11-11 1990-05-16 Medical Research Council Cloning immunoglobulin variable domain sequences.
US4941880A (en) 1987-06-19 1990-07-17 Bioject, Inc. Pre-filled ampule and non-invasive hypodermic injection device assembly
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US4975278A (en) 1988-02-26 1990-12-04 Bristol-Myers Company Antibody-enzyme conjugates in combination with prodrugs for the delivery of cytotoxic agents to tumor cells
EP0401384A1 (en) 1988-12-22 1990-12-12 Kirin-Amgen, Inc. Chemically modified granulocyte colony stimulating factor
US4978757A (en) 1984-02-21 1990-12-18 The Upjohn Company 1,2,8,8a-tetrahydrocyclopropa (C) pyrrolo [3,2-e)]-indol-4(5H)-ones and related compounds
US5013653A (en) 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5064413A (en) 1989-11-09 1991-11-12 Bioject, Inc. Needleless hypodermic injection device
US5070092A (en) 1989-07-03 1991-12-03 Kyowa Hakko Kogyo Co., Ltd. Pyrroloindole derivatives related to dc-88a compound
US5084468A (en) 1988-08-11 1992-01-28 Kyowa Hakko Kogyo Co., Ltd. Dc-88a derivatives
US5091513A (en) 1987-05-21 1992-02-25 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
WO1992003918A1 (en) 1990-08-29 1992-03-19 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5101038A (en) 1988-12-28 1992-03-31 Kyowa Hakko Kogyo Co., Ltd. Novel substance dc 113 and production thereof
US5132405A (en) 1987-05-21 1992-07-21 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US5179017A (en) 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US5187186A (en) 1989-07-03 1993-02-16 Kyowa Hakko Kogyo Co., Ltd. Pyrroloindole derivatives
EP0537575A1 (en) 1991-10-07 1993-04-21 Kyowa Hakko Kogyo Co., Ltd. Hydrobromide of DC-89 derivative having antitumor activity
WO1993012227A1 (en) 1991-12-17 1993-06-24 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US5258498A (en) 1987-05-21 1993-11-02 Creative Biomolecules, Inc. Polypeptide linkers for production of biosynthetic proteins
US5260203A (en) 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
WO1994010332A1 (en) 1992-11-04 1994-05-11 Medarex, Inc. HUMANIZED ANTIBODIES TO Fc RECEPTORS FOR IMMUNOGLOBULIN G ON HUMAN MONONUCLEAR PHAGOCYTES
US5312335A (en) 1989-11-09 1994-05-17 Bioject Inc. Needleless hypodermic injection device
US5332837A (en) 1986-12-19 1994-07-26 The Upjohn Company CC-1065 analogs
EP0616640A1 (en) 1991-12-02 1994-09-28 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
WO1994025585A1 (en) 1993-04-26 1994-11-10 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5374548A (en) 1986-05-02 1994-12-20 Genentech, Inc. Methods and compositions for the attachment of proteins to liposomes using a glycophospholipid anchor
WO1994029351A2 (en) 1993-06-16 1994-12-22 Celltech Limited Antibodies
US5383851A (en) 1992-07-24 1995-01-24 Bioject Inc. Needleless hypodermic injection device
US5399331A (en) 1985-06-26 1995-03-21 The Liposome Company, Inc. Method for protein-liposome coupling
US5416016A (en) 1989-04-03 1995-05-16 Purdue Research Foundation Method for enhancing transmembrane transport of exogenous molecules
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5476996A (en) 1988-06-14 1995-12-19 Lidak Pharmaceuticals Human immune system in non-human animal
US5476786A (en) 1987-05-21 1995-12-19 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
WO1996010405A1 (en) 1994-09-30 1996-04-11 Kyowa Hakko Kogyo Co., Ltd. Antitumor agent
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5545807A (en) 1988-10-12 1996-08-13 The Babraham Institute Production of antibodies from transgenic animals
US5587161A (en) 1992-07-23 1996-12-24 Zeneca Limited Prodrugs for antibody directed enzyme prodrug therapy
WO1997013852A1 (en) 1995-10-10 1997-04-17 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5624821A (en) 1987-03-18 1997-04-29 Scotgen Biopharmaceuticals Incorporated Antibodies with altered effector functions
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5641780A (en) 1994-04-22 1997-06-24 Kyowa Hakko Kogyo Co., Ltd. Pyrrolo-indole derivatives
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5677425A (en) 1987-09-04 1997-10-14 Celltech Therapeutics Limited Recombinant antibody
US5703080A (en) 1994-05-20 1997-12-30 Kyowa Hakko Kogyo Co., Ltd. Method for stabilizing duocarmycin derivatives
US5712375A (en) 1990-06-11 1998-01-27 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5714350A (en) 1992-03-09 1998-02-03 Protein Design Labs, Inc. Increasing antibody affinity by altering glycosylation in the immunoglobulin variable region
US5739350A (en) 1990-04-25 1998-04-14 Pharmacia & Upjohn Company CC-1065 analogs
US5760185A (en) 1992-11-28 1998-06-02 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Anti-feline herpes virus-1 recombinant antibody and gene fragment coding for said antibody
US5763566A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US5762905A (en) 1992-09-16 1998-06-09 The Scripps Research Institute Human neutralizing monoclonal antibodies to respiratory syncytial virus
WO1998024884A1 (en) 1996-12-02 1998-06-11 Genpharm International Transgenic non-human animals capable of producing heterologous antibodies
US5773435A (en) 1987-08-04 1998-06-30 Bristol-Myers Squibb Company Prodrugs for β-lactamase and uses thereof
US5789157A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5789650A (en) 1990-08-29 1998-08-04 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5814318A (en) 1990-08-29 1998-09-29 Genpharm International Inc. Transgenic non-human animals for producing heterologous antibodies
US5831077A (en) 1993-12-09 1998-11-03 Redmond; John William Glycosylhydrazines, preparation, immobilization and reactions of: glycoprotein analysis and O-glycan removal
US5831012A (en) 1994-01-14 1998-11-03 Pharmacia & Upjohn Aktiebolag Bacterial receptor structures
US5833943A (en) 1991-04-23 1998-11-10 Cancer Therapeutics Limited Minimum recognition unit of a pem mucin tandem repeat specific monoclonal antibody
US5864026A (en) 1990-06-11 1999-01-26 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5869046A (en) 1995-04-14 1999-02-09 Genentech, Inc. Altered polypeptides with increased half-life
US5874299A (en) 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5877397A (en) 1990-08-29 1999-03-02 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5885793A (en) 1991-12-02 1999-03-23 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
WO1999016873A1 (en) 1997-09-26 1999-04-08 Arne Skerra Anticalins
US5939598A (en) 1990-01-12 1999-08-17 Abgenix, Inc. Method of making transgenic mice lacking endogenous heavy chains
WO1999045962A1 (en) 1998-03-13 1999-09-16 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5969108A (en) 1990-07-10 1999-10-19 Medical Research Council Methods for producing members of specific binding pairs
WO1999054342A1 (en) 1998-04-20 1999-10-28 Pablo Umana Glycosylation engineering of antibodies for improving antibody-dependent cellular cytotoxicity
US6013443A (en) 1995-05-03 2000-01-11 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
WO2000026373A1 (en) 1998-11-03 2000-05-11 Babraham Institute MURINE EXPRESSION OF HUMAN Igμ LOCUS
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
WO2000033888A2 (en) 1998-12-11 2000-06-15 Coulter Pharmaceutical, Inc. Prodrug compounds and process for preparation thereof
US6090382A (en) 1996-02-09 2000-07-18 Basf Aktiengesellschaft Human antibodies that bind human TNFα
WO2000042072A2 (en) 1999-01-15 2000-07-20 Genentech, Inc. Polypeptide variants with altered effector function
US6114120A (en) 1995-05-03 2000-09-05 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US6121022A (en) 1995-04-14 2000-09-19 Genentech, Inc. Altered polypeptides with increased half-life
US6132722A (en) 1997-05-07 2000-10-17 Bristol-Myers Squibb Company Recombinant antibody-enzyme fusion proteins
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6156313A (en) 1994-01-04 2000-12-05 The Scripps Research Institute Human monoclonal antibodies to herpes simplex virus and methods therefor
US6162963A (en) 1990-01-12 2000-12-19 Abgenix, Inc. Generation of Xenogenetic antibodies
US6165745A (en) 1992-04-24 2000-12-26 Board Of Regents, The University Of Texas System Recombinant production of immunoglobulin-like domains in prokaryotic cells
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
WO2001009187A2 (en) 1999-07-29 2001-02-08 Medarex, Inc. Human monoclonal antibodies to her2/neu
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
WO2001014424A2 (en) 1999-08-24 2001-03-01 Medarex, Inc. Human ctla-4 antibodies and their uses
US6214345B1 (en) 1993-05-14 2001-04-10 Bristol-Myers Squibb Co. Lysosomal enzyme-cleavable antitumor drug conjugates
US6261774B1 (en) 1990-06-11 2001-07-17 Gilead Sciences, Inc. Truncation selex method
US6277375B1 (en) 1997-03-03 2001-08-21 Board Of Regents, The University Of Texas System Immunoglobulin-like domains with increased half-lives
US6281354B1 (en) 1997-05-22 2001-08-28 The Scripps Research Institute Analogs of duocarmycin and cc-1065
US6291158B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
WO2001095943A2 (en) 2000-06-14 2001-12-20 Medarex, Inc. Prodrug compounds with an oligopeptide having an isoleucine residue
WO2001095945A2 (en) 2000-06-14 2001-12-20 Medarex, Inc. Prodrug compounds cleavable by thimet oligopeptidase
WO2002000263A2 (en) 2000-06-14 2002-01-03 Medarex, Inc. Tripeptide prodrug compounds
EP1176195A1 (en) 1999-04-09 2002-01-30 Kyowa Hakko Kogyo Co., Ltd. Method for controlling the activity of immunologically functional molecule
WO2002020565A2 (en) 2000-09-08 2002-03-14 Universität Zürich Collections of repeat proteins comprising repeat modules
US6387620B1 (en) 1999-07-28 2002-05-14 Gilead Sciences, Inc. Transcription-free selex
WO2002043478A2 (en) 2000-11-30 2002-06-06 Medarex, Inc. Transgenic transchromosomal rodents for making human antibodies
WO2002083180A1 (en) 2001-03-23 2002-10-24 Syntarga B.V. Elongated and multiple spacers in activatible prodrugs
WO2002092812A1 (en) 2001-05-11 2002-11-21 Kirin Beer Kabushiki Kaisha ARTIFICIAL HUMAN CHROMOSOME CONTAINING HUMAN ANTIBODY μ LIGHT CHAIN GENE
WO2002092780A2 (en) 2001-05-17 2002-11-21 Diversa Corporation Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof
WO2002096910A1 (en) 2001-05-31 2002-12-05 Medarex, Inc. Cytotoxins, prodrugs, linkers and stabilizers useful therefor
WO2002100353A2 (en) 2001-06-11 2002-12-19 Medarex, Inc. Cd10-activated prodrug compounds
WO2003002609A2 (en) 2001-06-28 2003-01-09 Domantis Limited Dual-specific ligand and its use
WO2003022806A2 (en) 2001-09-07 2003-03-20 The Scripps Research Institute Cbi analogues of cc-1065 and the duocarmycins
US6548530B1 (en) 1995-10-03 2003-04-15 The Scripps Research Institute CBI analogs of CC-1065 and the duocarmycins
WO2003035835A2 (en) 2001-10-25 2003-05-01 Genentech, Inc. Glycoprotein compositions
US20030096743A1 (en) 2001-09-24 2003-05-22 Seattle Genetics, Inc. p-Amidobenzylethers in drug delivery agents
US20030153043A1 (en) 1997-05-21 2003-08-14 Biovation Limited Method for the production of non-immunogenic proteins
WO2003074679A2 (en) 2002-03-01 2003-09-12 Xencor Antibody optimization
WO2004003019A2 (en) 2002-06-28 2004-01-08 Domantis Limited Immunoglobin single variant antigen-binding domains and dual-specific constructs
US6696245B2 (en) 1997-10-20 2004-02-24 Domantis Limited Methods for selecting functional polypeptides
US6737056B1 (en) 1999-01-15 2004-05-18 Genentech, Inc. Polypeptide variants with altered effector function
WO2004041867A2 (en) 2002-11-08 2004-05-21 Ablynx N.V. Camelidae antibodies against imminoglobulin e and use thereof for the treatment of allergic disorders
US20040110704A1 (en) 2002-04-09 2004-06-10 Kyowa Hakko Kogyo Co., Ltd. Cells of which genome is modified
WO2004058821A2 (en) 2002-12-27 2004-07-15 Domantis Limited Dual specific single domain antibodies specific for a ligand and for the receptor of the ligand
US6765087B1 (en) 1992-08-21 2004-07-20 Vrije Universiteit Brussel Immunoglobulins devoid of light chains
US20040175756A1 (en) 2001-04-26 2004-09-09 Avidia Research Institute Methods for using combinatorial libraries of monomer domains
US6794132B2 (en) 1999-10-02 2004-09-21 Biosite, Inc. Human antibodies
WO2004081026A2 (en) 2003-06-30 2004-09-23 Domantis Limited Polypeptides
US6818216B2 (en) 2000-11-28 2004-11-16 Medimmune, Inc. Anti-RSV antibodies
WO2004101790A1 (en) 2003-05-14 2004-11-25 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
US6827925B1 (en) 1998-07-02 2004-12-07 Cambridge Antibody Technology Limited Specific binding proteins including antibodies which bind to the necrotic center of tumors, and uses thereof
US6838254B1 (en) 1993-04-29 2005-01-04 Conopco, Inc. Production of antibodies or (functionalized) fragments thereof derived from heavy chain immunoglobulins of camelidae
US20050048512A1 (en) 2001-04-26 2005-03-03 Avidia Research Institute Combinatorial libraries of monomer domains
US20050053973A1 (en) 2001-04-26 2005-03-10 Avidia Research Institute Novel proteins with targeted binding
US20050089932A1 (en) 2001-04-26 2005-04-28 Avidia Research Institute Novel proteins with targeted binding
US6914128B1 (en) 1999-03-25 2005-07-05 Abbott Gmbh & Co. Kg Human antibodies that bind human IL-12 and methods for producing
US20050164301A1 (en) 2003-10-24 2005-07-28 Avidia Research Institute LDL receptor class A and EGF domain monomers and multimers
US6951646B1 (en) 1998-07-21 2005-10-04 Genmab A/S Anti hepatitis C virus antibody and uses thereof
US20050221384A1 (en) 2001-04-26 2005-10-06 Avidia Research Institute Combinatorial libraries of monomer domains
US20060008844A1 (en) 2004-06-17 2006-01-12 Avidia Research Institute c-Met kinase binding proteins
WO2006079372A1 (en) 2005-01-31 2006-08-03 Ablynx N.V. Method for generating variable domain sequences of heavy chain antibodies
WO2006089294A2 (en) 2005-02-18 2006-08-24 Origen Therapeutics, Inc. Tissue specific expression of antibodies in chickens
US20060223114A1 (en) 2001-04-26 2006-10-05 Avidia Research Institute Protein scaffolds and uses thereof
US20060234299A1 (en) 2004-11-16 2006-10-19 Avidia Research Institute Protein scaffolds and uses thereof
US20060286603A1 (en) 2001-04-26 2006-12-21 Avidia Research Institute Combinatorial libraries of monomer domains
WO2007055916A2 (en) 2005-11-07 2007-05-18 The Rockefeller University Reagents, methods and systems for selecting a cytotoxic antibody or variant thereof
WO2007059404A2 (en) 2005-11-10 2007-05-24 Medarex, Inc. Duocarmycin derivatives as novel cytotoxic compounds and conjugates
WO2007059782A1 (en) 2005-11-28 2007-05-31 Genmab A/S Recombinant monovalent antibodies and methods for production thereof
WO2007084926A2 (en) 2006-01-17 2007-07-26 Biolex Therapeutics, Inc. Compositions and methods for humanization and optimization of n-glycans in plants
US20070191272A1 (en) 2005-09-27 2007-08-16 Stemmer Willem P Proteinaceous pharmaceuticals and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11505704A (en) * 1995-05-17 1999-05-25 リージェンツ・オブ・ザ・ユニバーシティ・オブ・ミネソタ Immunoconjugates Containing Single Chain Variable Region Fragments of Anti-CD-19 Antibodies
EP1073464B1 (en) * 1998-04-28 2004-10-06 Smithkline Beecham Corporation Monoclonal antibodies with reduced immunogenicity
US7595378B2 (en) * 2001-06-13 2009-09-29 Genmab A/S Human monoclonal antibodies to epidermal growth factor receptor (EGFR)
US7135174B2 (en) * 2002-01-07 2006-11-14 Amgen Fremont, Inc. Antibodies directed to PDGFD and uses thereof
US20040258678A1 (en) * 2002-02-22 2004-12-23 Genentech, Inc. Compositions and methods for the treatment of immune related diseases
AU2003295411A1 (en) * 2002-11-07 2004-06-03 Celltech R & D Human monoclonal antibodies to heparanase
CA2534639C (en) * 2003-07-31 2013-07-30 Immunomedics, Inc. Anti-cd19 antibodies
EP2360186B1 (en) * 2004-04-13 2017-08-30 F. Hoffmann-La Roche AG Anti-P-selectin antibodies
CN102863532A (en) * 2004-06-21 2013-01-09 米德列斯公司 Interferon alpha receptor 1 antibodies and their uses
AU2006214121B9 (en) * 2005-02-15 2013-02-14 Duke University Anti-CD19 antibodies and uses in oncology
JP3857712B2 (en) * 2005-05-20 2006-12-13 株式会社コナミデジタルエンタテインメント Game system
EP1899379B1 (en) * 2005-06-20 2018-04-11 E. R. Squibb & Sons, L.L.C. Cd19 antibodies and their uses
CN100543035C (en) * 2006-09-14 2009-09-23 中国医学科学院血液学研究所 Be used for the treatment of bone-marrow-derived lymphocyte leukemia, lymphadenomatous B7.1-CD19scFv fusion gene engineering albumen and uses thereof

Patent Citations (203)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4391904A (en) 1979-12-26 1983-07-05 Syva Company Test strip kits in immunoassays and compositions therein
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US5179017A (en) 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4475196A (en) 1981-03-06 1984-10-02 Zor Clair G Instrument for locating faults in aircraft passenger reading light and attendant call control system
US4447233A (en) 1981-04-10 1984-05-08 Parker-Hannifin Corporation Medication infusion pump
US4439196A (en) 1982-03-18 1984-03-27 Merck & Co., Inc. Osmotic drug delivery system
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
US4447224A (en) 1982-09-20 1984-05-08 Infusaid Corporation Variable flow implantable infusion apparatus
US4487603A (en) 1982-11-26 1984-12-11 Cordis Corporation Implantable microinfusion pump system
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4486194A (en) 1983-06-08 1984-12-04 James Ferrara Therapeutic device for administering medicaments through the skin
US4978757A (en) 1984-02-21 1990-12-18 The Upjohn Company 1,2,8,8a-tetrahydrocyclopropa (C) pyrrolo [3,2-e)]-indol-4(5H)-ones and related compounds
US4912227A (en) 1984-02-21 1990-03-27 The Upjohn Company 1,2,8,8A-tetrahydrocyclopropa(c)pyrrolo(3,2-e)-indol-4-(5H)-ones and related compounds
EP0154316A2 (en) 1984-03-06 1985-09-11 Takeda Chemical Industries, Ltd. Chemically modified lymphokine and production thereof
US4596556A (en) 1985-03-25 1986-06-24 Bioject, Inc. Hypodermic injection apparatus
US5399331A (en) 1985-06-26 1995-03-21 The Liposome Company, Inc. Method for protein-liposome coupling
WO1987004462A1 (en) 1986-01-23 1987-07-30 Celltech Limited Recombinant dna sequences, vectors containing them and method for the use thereof
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US5374548A (en) 1986-05-02 1994-12-20 Genentech, Inc. Methods and compositions for the attachment of proteins to liposomes using a glycophospholipid anchor
WO1988000052A1 (en) 1986-07-07 1988-01-14 Trustees Of Dartmouth College Monoclonal antibodies to fc receptor
US4954617A (en) 1986-07-07 1990-09-04 Trustees Of Dartmouth College Monoclonal antibodies to FC receptors for immunoglobulin G on human mononuclear phagocytes
US4881175A (en) 1986-09-02 1989-11-14 Genex Corporation Computer based system and method for determining and displaying possible chemical structures for converting double- or multiple-chain polypeptides to single-chain polypeptides
US5455030A (en) 1986-09-02 1995-10-03 Enzon Labs, Inc. Immunotheraphy using single chain polypeptide binding molecules
US5260203A (en) 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
US5332837A (en) 1986-12-19 1994-07-26 The Upjohn Company CC-1065 analogs
US5624821A (en) 1987-03-18 1997-04-29 Scotgen Biopharmaceuticals Incorporated Antibodies with altered effector functions
US5648260A (en) 1987-03-18 1997-07-15 Scotgen Biopharmaceuticals Incorporated DNA encoding antibodies with altered effector functions
US5013653A (en) 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5258498A (en) 1987-05-21 1993-11-02 Creative Biomolecules, Inc. Polypeptide linkers for production of biosynthetic proteins
US5482858A (en) 1987-05-21 1996-01-09 Creative Biomolecules, Inc. Polypeptide linkers for production of biosynthetic proteins
US5091513A (en) 1987-05-21 1992-02-25 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US5476786A (en) 1987-05-21 1995-12-19 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US5132405A (en) 1987-05-21 1992-07-21 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US4790824A (en) 1987-06-19 1988-12-13 Bioject, Inc. Non-invasive hypodermic injection device
US4941880A (en) 1987-06-19 1990-07-17 Bioject, Inc. Pre-filled ampule and non-invasive hypodermic injection device assembly
WO1989001036A1 (en) 1987-07-23 1989-02-09 Celltech Limited Recombinant dna expression vectors
US5773435A (en) 1987-08-04 1998-06-30 Bristol-Myers Squibb Company Prodrugs for β-lactamase and uses thereof
US5677425A (en) 1987-09-04 1997-10-14 Celltech Therapeutics Limited Recombinant antibody
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US4975278A (en) 1988-02-26 1990-12-04 Bristol-Myers Company Antibody-enzyme conjugates in combination with prodrugs for the delivery of cytotoxic agents to tumor cells
EP0338841A1 (en) 1988-04-18 1989-10-25 Celltech Limited Recombinant DNA methods, vectors and host cells
US5476996A (en) 1988-06-14 1995-12-19 Lidak Pharmaceuticals Human immune system in non-human animal
US5698767A (en) 1988-06-14 1997-12-16 Lidak Pharmaceuticals Human immune system in non-human animal
US5084468A (en) 1988-08-11 1992-01-28 Kyowa Hakko Kogyo Co., Ltd. Dc-88a derivatives
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5571698A (en) 1988-09-02 1996-11-05 Protein Engineering Corporation Directed evolution of novel binding proteins
US5403484A (en) 1988-09-02 1995-04-04 Protein Engineering Corporation Viruses expressing chimeric binding proteins
US5545807A (en) 1988-10-12 1996-08-13 The Babraham Institute Production of antibodies from transgenic animals
EP0368684A1 (en) 1988-11-11 1990-05-16 Medical Research Council Cloning immunoglobulin variable domain sequences.
US20040110941A2 (en) 1988-11-11 2004-06-10 Medical Research Council Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors
EP0401384A1 (en) 1988-12-22 1990-12-12 Kirin-Amgen, Inc. Chemically modified granulocyte colony stimulating factor
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6180370B1 (en) 1988-12-28 2001-01-30 Protein Design Labs, Inc. Humanized immunoglobulins and methods of making the same
US5585089A (en) 1988-12-28 1996-12-17 Protein Design Labs, Inc. Humanized immunoglobulins
US5101038A (en) 1988-12-28 1992-03-31 Kyowa Hakko Kogyo Co., Ltd. Novel substance dc 113 and production thereof
US5693762A (en) 1988-12-28 1997-12-02 Protein Design Labs, Inc. Humanized immunoglobulins
US5416016A (en) 1989-04-03 1995-05-16 Purdue Research Foundation Method for enhancing transmembrane transport of exogenous molecules
US6291158B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
US5070092A (en) 1989-07-03 1991-12-03 Kyowa Hakko Kogyo Co., Ltd. Pyrroloindole derivatives related to dc-88a compound
US5187186A (en) 1989-07-03 1993-02-16 Kyowa Hakko Kogyo Co., Ltd. Pyrroloindole derivatives
US5064413A (en) 1989-11-09 1991-11-12 Bioject, Inc. Needleless hypodermic injection device
US5312335A (en) 1989-11-09 1994-05-17 Bioject Inc. Needleless hypodermic injection device
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US5939598A (en) 1990-01-12 1999-08-17 Abgenix, Inc. Method of making transgenic mice lacking endogenous heavy chains
US6162963A (en) 1990-01-12 2000-12-19 Abgenix, Inc. Generation of Xenogenetic antibodies
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6114598A (en) 1990-01-12 2000-09-05 Abgenix, Inc. Generation of xenogeneic antibodies
US5739350A (en) 1990-04-25 1998-04-14 Pharmacia & Upjohn Company CC-1065 analogs
US5580717A (en) 1990-05-01 1996-12-03 Affymax Technologies N.V. Recombinant library screening methods
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5712375A (en) 1990-06-11 1998-01-27 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5864026A (en) 1990-06-11 1999-01-26 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5789157A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US6261774B1 (en) 1990-06-11 2001-07-17 Gilead Sciences, Inc. Truncation selex method
US5763566A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US5969108A (en) 1990-07-10 1999-10-19 Medical Research Council Methods for producing members of specific binding pairs
EP1433846A2 (en) 1990-07-10 2004-06-30 Cambridge Antibody Technology LTD Phagemid-based method of producing filamentous bacteriophage particles displaying antibody molecules and the corresponding bacteriophage particles.
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
WO1992003918A1 (en) 1990-08-29 1992-03-19 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5877397A (en) 1990-08-29 1999-03-02 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5569825A (en) 1990-08-29 1996-10-29 Genpharm International Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5874299A (en) 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5814318A (en) 1990-08-29 1998-09-29 Genpharm International Inc. Transgenic non-human animals for producing heterologous antibodies
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5789650A (en) 1990-08-29 1998-08-04 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5833943A (en) 1991-04-23 1998-11-10 Cancer Therapeutics Limited Minimum recognition unit of a pem mucin tandem repeat specific monoclonal antibody
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
EP0537575A1 (en) 1991-10-07 1993-04-21 Kyowa Hakko Kogyo Co., Ltd. Hydrobromide of DC-89 derivative having antitumor activity
US6555313B1 (en) 1991-12-02 2003-04-29 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
US6593081B1 (en) 1991-12-02 2003-07-15 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
US6521404B1 (en) 1991-12-02 2003-02-18 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
EP0616640A1 (en) 1991-12-02 1994-09-28 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
US6582915B1 (en) 1991-12-02 2003-06-24 Medical Research Council Production of anti-self bodies from antibody segment repertories and displayed on phage
US5885793A (en) 1991-12-02 1999-03-23 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
US6544731B1 (en) 1991-12-02 2003-04-08 Medical Research Council Production of anti-self antibodies from antibody segment repertories and displayed on phage
WO1993012227A1 (en) 1991-12-17 1993-06-24 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5714350A (en) 1992-03-09 1998-02-03 Protein Design Labs, Inc. Increasing antibody affinity by altering glycosylation in the immunoglobulin variable region
US6350861B1 (en) 1992-03-09 2002-02-26 Protein Design Labs, Inc. Antibodies with increased binding affinity
US6165745A (en) 1992-04-24 2000-12-26 Board Of Regents, The University Of Texas System Recombinant production of immunoglobulin-like domains in prokaryotic cells
US5660829A (en) 1992-07-23 1997-08-26 Zeneca Limited Process for antibody directed enzyme prodrug therapy
US5587161A (en) 1992-07-23 1996-12-24 Zeneca Limited Prodrugs for antibody directed enzyme prodrug therapy
US5399163A (en) 1992-07-24 1995-03-21 Bioject Inc. Needleless hypodermic injection methods and device
US5383851A (en) 1992-07-24 1995-01-24 Bioject Inc. Needleless hypodermic injection device
US6765087B1 (en) 1992-08-21 2004-07-20 Vrije Universiteit Brussel Immunoglobulins devoid of light chains
US5762905A (en) 1992-09-16 1998-06-09 The Scripps Research Institute Human neutralizing monoclonal antibodies to respiratory syncytial virus
WO1994010332A1 (en) 1992-11-04 1994-05-11 Medarex, Inc. HUMANIZED ANTIBODIES TO Fc RECEPTORS FOR IMMUNOGLOBULIN G ON HUMAN MONONUCLEAR PHAGOCYTES
US5760185A (en) 1992-11-28 1998-06-02 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Anti-feline herpes virus-1 recombinant antibody and gene fragment coding for said antibody
WO1994025585A1 (en) 1993-04-26 1994-11-10 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6838254B1 (en) 1993-04-29 2005-01-04 Conopco, Inc. Production of antibodies or (functionalized) fragments thereof derived from heavy chain immunoglobulins of camelidae
US6214345B1 (en) 1993-05-14 2001-04-10 Bristol-Myers Squibb Co. Lysosomal enzyme-cleavable antitumor drug conjugates
WO1994029351A2 (en) 1993-06-16 1994-12-22 Celltech Limited Antibodies
US5831077A (en) 1993-12-09 1998-11-03 Redmond; John William Glycosylhydrazines, preparation, immobilization and reactions of: glycoprotein analysis and O-glycan removal
US6156313A (en) 1994-01-04 2000-12-05 The Scripps Research Institute Human monoclonal antibodies to herpes simplex virus and methods therefor
US5831012A (en) 1994-01-14 1998-11-03 Pharmacia & Upjohn Aktiebolag Bacterial receptor structures
US5641780A (en) 1994-04-22 1997-06-24 Kyowa Hakko Kogyo Co., Ltd. Pyrrolo-indole derivatives
US5703080A (en) 1994-05-20 1997-12-30 Kyowa Hakko Kogyo Co., Ltd. Method for stabilizing duocarmycin derivatives
WO1996010405A1 (en) 1994-09-30 1996-04-11 Kyowa Hakko Kogyo Co., Ltd. Antitumor agent
US5869046A (en) 1995-04-14 1999-02-09 Genentech, Inc. Altered polypeptides with increased half-life
US6121022A (en) 1995-04-14 2000-09-19 Genentech, Inc. Altered polypeptides with increased half-life
US6114120A (en) 1995-05-03 2000-09-05 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US6613526B2 (en) 1995-05-03 2003-09-02 Gilead Sciences, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US6376474B1 (en) 1995-05-03 2002-04-23 Gilead Sciences, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US6013443A (en) 1995-05-03 2000-01-11 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US6548530B1 (en) 1995-10-03 2003-04-15 The Scripps Research Institute CBI analogs of CC-1065 and the duocarmycins
WO1997013852A1 (en) 1995-10-10 1997-04-17 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6090382A (en) 1996-02-09 2000-07-18 Basf Aktiengesellschaft Human antibodies that bind human TNFα
WO1998024884A1 (en) 1996-12-02 1998-06-11 Genpharm International Transgenic non-human animals capable of producing heterologous antibodies
US6277375B1 (en) 1997-03-03 2001-08-21 Board Of Regents, The University Of Texas System Immunoglobulin-like domains with increased half-lives
US6132722A (en) 1997-05-07 2000-10-17 Bristol-Myers Squibb Company Recombinant antibody-enzyme fusion proteins
US20030153043A1 (en) 1997-05-21 2003-08-14 Biovation Limited Method for the production of non-immunogenic proteins
US6281354B1 (en) 1997-05-22 2001-08-28 The Scripps Research Institute Analogs of duocarmycin and cc-1065
US7250297B1 (en) 1997-09-26 2007-07-31 Pieris Ag Anticalins
WO1999016873A1 (en) 1997-09-26 1999-04-08 Arne Skerra Anticalins
US6696245B2 (en) 1997-10-20 2004-02-24 Domantis Limited Methods for selecting functional polypeptides
WO1999045962A1 (en) 1998-03-13 1999-09-16 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
WO1999054342A1 (en) 1998-04-20 1999-10-28 Pablo Umana Glycosylation engineering of antibodies for improving antibody-dependent cellular cytotoxicity
US6827925B1 (en) 1998-07-02 2004-12-07 Cambridge Antibody Technology Limited Specific binding proteins including antibodies which bind to the necrotic center of tumors, and uses thereof
US6951646B1 (en) 1998-07-21 2005-10-04 Genmab A/S Anti hepatitis C virus antibody and uses thereof
WO2000026373A1 (en) 1998-11-03 2000-05-11 Babraham Institute MURINE EXPRESSION OF HUMAN Igμ LOCUS
WO2000033888A2 (en) 1998-12-11 2000-06-15 Coulter Pharmaceutical, Inc. Prodrug compounds and process for preparation thereof
US6737056B1 (en) 1999-01-15 2004-05-18 Genentech, Inc. Polypeptide variants with altered effector function
WO2000042072A2 (en) 1999-01-15 2000-07-20 Genentech, Inc. Polypeptide variants with altered effector function
US6914128B1 (en) 1999-03-25 2005-07-05 Abbott Gmbh & Co. Kg Human antibodies that bind human IL-12 and methods for producing
EP1176195A1 (en) 1999-04-09 2002-01-30 Kyowa Hakko Kogyo Co., Ltd. Method for controlling the activity of immunologically functional molecule
US7214775B2 (en) 1999-04-09 2007-05-08 Kyowa Hakko Kogyo Co., Ltd. Method of modulating the activity of functional immune molecules
US6387620B1 (en) 1999-07-28 2002-05-14 Gilead Sciences, Inc. Transcription-free selex
WO2001009187A2 (en) 1999-07-29 2001-02-08 Medarex, Inc. Human monoclonal antibodies to her2/neu
WO2001014424A2 (en) 1999-08-24 2001-03-01 Medarex, Inc. Human ctla-4 antibodies and their uses
US6794132B2 (en) 1999-10-02 2004-09-21 Biosite, Inc. Human antibodies
WO2001095943A2 (en) 2000-06-14 2001-12-20 Medarex, Inc. Prodrug compounds with an oligopeptide having an isoleucine residue
WO2002000263A2 (en) 2000-06-14 2002-01-03 Medarex, Inc. Tripeptide prodrug compounds
WO2001095945A2 (en) 2000-06-14 2001-12-20 Medarex, Inc. Prodrug compounds cleavable by thimet oligopeptidase
WO2002020565A2 (en) 2000-09-08 2002-03-14 Universität Zürich Collections of repeat proteins comprising repeat modules
US20040132028A1 (en) 2000-09-08 2004-07-08 Stumpp Michael Tobias Collection of repeat proteins comprising repeat modules
US6818216B2 (en) 2000-11-28 2004-11-16 Medimmune, Inc. Anti-RSV antibodies
WO2002043478A2 (en) 2000-11-30 2002-06-06 Medarex, Inc. Transgenic transchromosomal rodents for making human antibodies
WO2002083180A1 (en) 2001-03-23 2002-10-24 Syntarga B.V. Elongated and multiple spacers in activatible prodrugs
US20060223114A1 (en) 2001-04-26 2006-10-05 Avidia Research Institute Protein scaffolds and uses thereof
US20050048512A1 (en) 2001-04-26 2005-03-03 Avidia Research Institute Combinatorial libraries of monomer domains
US20060286603A1 (en) 2001-04-26 2006-12-21 Avidia Research Institute Combinatorial libraries of monomer domains
US20050221384A1 (en) 2001-04-26 2005-10-06 Avidia Research Institute Combinatorial libraries of monomer domains
US20050089932A1 (en) 2001-04-26 2005-04-28 Avidia Research Institute Novel proteins with targeted binding
US20040175756A1 (en) 2001-04-26 2004-09-09 Avidia Research Institute Methods for using combinatorial libraries of monomer domains
US20050053973A1 (en) 2001-04-26 2005-03-10 Avidia Research Institute Novel proteins with targeted binding
WO2002092812A1 (en) 2001-05-11 2002-11-21 Kirin Beer Kabushiki Kaisha ARTIFICIAL HUMAN CHROMOSOME CONTAINING HUMAN ANTIBODY μ LIGHT CHAIN GENE
WO2002092780A2 (en) 2001-05-17 2002-11-21 Diversa Corporation Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof
US20030073852A1 (en) 2001-05-31 2003-04-17 Medarex, Inc. Disulfide prodrugs and linkers and stabilizers useful therefor
WO2002096910A1 (en) 2001-05-31 2002-12-05 Medarex, Inc. Cytotoxins, prodrugs, linkers and stabilizers useful therefor
US6989452B2 (en) 2001-05-31 2006-01-24 Medarex, Inc. Disulfide prodrugs and linkers and stabilizers useful therefor
US20030050331A1 (en) 2001-05-31 2003-03-13 Medarex Inc. Cytotoxic agents
US20030064984A1 (en) 2001-05-31 2003-04-03 Medarex, Inc. Peptidyl prodrugs and linkers and stabilizers useful therefor
WO2002100353A2 (en) 2001-06-11 2002-12-19 Medarex, Inc. Cd10-activated prodrug compounds
US20040087497A1 (en) 2001-06-11 2004-05-06 Bebbington Christopher R. CD10-activated prodrug compounds
WO2003002609A2 (en) 2001-06-28 2003-01-09 Domantis Limited Dual-specific ligand and its use
WO2003022806A2 (en) 2001-09-07 2003-03-20 The Scripps Research Institute Cbi analogues of cc-1065 and the duocarmycins
US20030096743A1 (en) 2001-09-24 2003-05-22 Seattle Genetics, Inc. p-Amidobenzylethers in drug delivery agents
US20030130189A1 (en) 2001-09-24 2003-07-10 Senter Peter D. P-amidobenzylethers in drug delivery agents
US20070020260A1 (en) 2001-10-25 2007-01-25 Genentech, Inc. Glycoprotein compositions
WO2003035835A2 (en) 2001-10-25 2003-05-01 Genentech, Inc. Glycoprotein compositions
WO2003074679A2 (en) 2002-03-01 2003-09-12 Xencor Antibody optimization
US20040110704A1 (en) 2002-04-09 2004-06-10 Kyowa Hakko Kogyo Co., Ltd. Cells of which genome is modified
WO2004003019A2 (en) 2002-06-28 2004-01-08 Domantis Limited Immunoglobin single variant antigen-binding domains and dual-specific constructs
WO2004041867A2 (en) 2002-11-08 2004-05-21 Ablynx N.V. Camelidae antibodies against imminoglobulin e and use thereof for the treatment of allergic disorders
WO2004058821A2 (en) 2002-12-27 2004-07-15 Domantis Limited Dual specific single domain antibodies specific for a ligand and for the receptor of the ligand
WO2004101790A1 (en) 2003-05-14 2004-11-25 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
WO2004081026A2 (en) 2003-06-30 2004-09-23 Domantis Limited Polypeptides
WO2005035572A2 (en) 2003-10-08 2005-04-21 Domantis Limited Antibody compositions and methods
US20050164301A1 (en) 2003-10-24 2005-07-28 Avidia Research Institute LDL receptor class A and EGF domain monomers and multimers
US20060177831A1 (en) 2004-06-17 2006-08-10 Avidia Research Institute c-MET kinase binding proteins
US20060008844A1 (en) 2004-06-17 2006-01-12 Avidia Research Institute c-Met kinase binding proteins
US20060234299A1 (en) 2004-11-16 2006-10-19 Avidia Research Institute Protein scaffolds and uses thereof
WO2006079372A1 (en) 2005-01-31 2006-08-03 Ablynx N.V. Method for generating variable domain sequences of heavy chain antibodies
WO2006089294A2 (en) 2005-02-18 2006-08-24 Origen Therapeutics, Inc. Tissue specific expression of antibodies in chickens
US20070191272A1 (en) 2005-09-27 2007-08-16 Stemmer Willem P Proteinaceous pharmaceuticals and uses thereof
WO2007055916A2 (en) 2005-11-07 2007-05-18 The Rockefeller University Reagents, methods and systems for selecting a cytotoxic antibody or variant thereof
WO2007059404A2 (en) 2005-11-10 2007-05-24 Medarex, Inc. Duocarmycin derivatives as novel cytotoxic compounds and conjugates
WO2007059782A1 (en) 2005-11-28 2007-05-31 Genmab A/S Recombinant monovalent antibodies and methods for production thereof
WO2007084926A2 (en) 2006-01-17 2007-07-26 Biolex Therapeutics, Inc. Compositions and methods for humanization and optimization of n-glycans in plants

Non-Patent Citations (171)

* Cited by examiner, † Cited by third party
Title
"Current Protocols in Molecular Biology", 1987, GREENE PUBLISHING AND WILEY INTERSCIENCE
"FUNDAMENTAL IMMUNOLOGY", 1993
"POLYMERIC DRUGS AND DRUG DELIVERY SYSTEMS", vol. 469, 1991, AMERICAN CHEMICAL SOCIETY
"Sustained and Controlled Release Drug Delivery Systems", 1978, MARCEL DEKKER, INC.
ADIB-CONQUY ET AL., INT. IMMUNOL., vol. 10, 1998, pages 341 - 6
ALEXANDER AJ; HUGHES DE, ANAL CHEM, vol. 67, 1995, pages 3626 - 32
ALLEN, T.M., NAT. REV. CANCER, vol. 2, 2002, pages 750 - 763
ALTSCHUL ET AL., J MOL. BIOL., vol. 215, 1990, pages 403 - 10
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, no. 17, 1997, pages 3389 - 3402
ALTSCHUL ET AL., NUCLEIC ACIDS RESEARCH, vol. 25, 1997, pages 3389 - 3402
BAE ET AL., DRUGS EXP. CLIN. RES., vol. 29, 2004, pages 15 - 23
BALDWIN ET AL., BIOCHEMISTRY, vol. 29, 1990, pages 5509 - 15
BARBAS ET AL., J AM. CHEM. SOC., vol. 116, 1994, pages 2161 - 2162
BARBAS ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 92, 1995, pages 2529 - 2533
BASSET ET AL., SCANDINAVIAN JOURNAL OF IMMUNOLOGY, vol. 51, no. 3, 2000, pages 307 - 311
BEERS ET AL., CLIN. CAN. RES., vol. 6, 2000, pages 2835 - 43
BEIBOER ET AL., J MOL. BIOL., vol. 296, 2000, pages 833 - 849
BEREZOV ET AL., BIAJOURNAL 8: SCIENTIFIC REVIEW, 2001, pages 8
BERGE, S.M. ET AL., J PHARM. SCI., vol. 66, 1977, pages 1 - 19
BIRD ET AL., SCIENCE, vol. 242, 1988, pages 423 - 426
BOGER ET AL., ANGEW. CHEM. INT. ED. ENGL., vol. 35, 1996, pages 1438
BOGER ET AL., CHEM. REV., vol. 97, 1997, pages 787
BOSS, M. A.; WOOD, C. R., IMMUNOLOGY TODAY, vol. 6, 1985, pages 12 - 13
BOURGEOIS ET AL., J VIROL, vol. 72, 1998, pages 807 - 10
BOUVIER ET AL., METH. ENZYMOL., vol. 248, 1995, pages 614
BRENNAN ET AL., SCIENCE, vol. 229, 1985, pages 81 - 83
BRISCOE ET AL., AM. J PHYSIOL., vol. 123, no. 3, 1995, pages 13 4
BRUMMELL ET AL., BIOCHEM, vol. 32, 1993, pages 1180 - 8
BUNDGAARD ET AL., J MED. CHEM., vol. 31, 1988, pages 2066
CARL ET AL., J. MED. CHEM. LETT., vol. 24, 1981, pages 479
CHAU ET AL., BIOCONJUGATE CHEM., vol. 15, 2004, pages 931 - 941
CHEN ET AL., EMBO J., vol. 12, 1993, pages 811 - 820
CHEN ET AL., PHARM RES, vol. 20, 2003, pages 1952 - 60
CHEN, J. ET AL., EMBO J., vol. 12, 1993, pages 821 - 830
CHEN, J. ET AL., INTERNATIONAL IMMUNOLOGY, vol. 5, 1993, pages 647 - 656
CHOI ET AL., NATURE GENETICS, vol. 4, 1993, pages 117 - 123
COUSSENS ET AL., GENES AND DEVELOPMENT, vol. 13, no. 11, 1999, pages 1382 - 97
COX, J. P. L. ET AL.: "A Directory of Human Germ-line VH Segments Reveals a Strong Bias in their Usage", EUR. J IMMUNOL., vol. 24, 1994, pages 827 - 836
DANO ET AL., ADV. CANCER. RES., vol. 44, 1985, pages 139
DE GROOT ET AL., J. MED. CHEM., vol. 42, 1999, pages 5277
DE GROOT ET AL., J. MED. CHEM., vol. 66, 2001, pages 8815
DE GROOT ET AL., J. ORG. CHEM., vol. 43, 2000, pages 3093
DE WILDT ET AL., PROTO ENG., vol. 10, 1997, pages 835 - 41
DITZEL ET AL., J IMMUNOL., vol. 157, 1996, pages 739 - 749
DUBOWCHIK ET AL., BIOORG & MED. CHEM. LETT., vol. 8, 1998, pages 3347
DUNN ET AL., METH. ENZYMOL., vol. 241, 1994, pages 254
E. MEYERS; W. MILLER, COMPUT. APPL. BIOSCI., vol. 4, 1988, pages 11 - 17
FEENEY ET AL.: "Modification of Proteins; Advances in Chemistry Series", vol. 198, 1982, AMERICAN CHEMICAL SOCIETY
FISHWILD ET AL., NATURE BIOTECHNOLOGY, vol. 14, 1996, pages 845 - 851
FISHWILD, D. ET AL., NATURE BIOTECHNOLOGY, vol. 14, 1996, pages 845 - 851
FUJIMOTO ET AL., SEMIN IMMUNOL., vol. 10, 1998, pages 267
GALA FA; MORRISON SL, JLMMUNOL, vol. 172, 2004, pages 5489 - 94
GHIRLANDO ET AL., IMMUNOL LETT, vol. 68, 1999, pages 47 - 52
GLENNIE ET AL., J IMMUNOL., vol. 139, 1987, pages 2367 - 2375
GRAZIANO, R.F. ET AL., J IMMUNOL, vol. 155, no. 10, 1995, pages 4996 - 5002
GREENE ET AL.: "Protective Groups in Organic Synthesis", 1991, JOHN WILEY & SONS
HALL ET AL., J IMMUNOL., vol. 149, 1992, pages 1605 - 12
HANKA ET AL., J. ANTIBIOT., vol. 31, 1978, pages 1211
HARDING, F.; LONBERG, N., ANN. N.Y. ACAD SCI., vol. 764, 1995, pages 536 - 546
HARDY ET AL.: "Amyloid Protein Precursor in Development, Aging, and Alzheimer's Disease", 1994, pages: 190 - 198
HASEGAWA ET AL., JLMMUNOL, vol. 167, 2001, pages 3190
HE ET AL., JRHEUMATOL, vol. 28, 2001, pages 2168
HERMANSON: "Bioconjugate Techniques", 1996, ACADEMIC PRESS
HUNT ET AL., J CHROMATOGR A, vol. 800, 1998, pages 355 - 67
HURLEY ET AL., SCIENCE, vol. 226, 1984, pages 843
HUSTON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 5879 - 5883
IGARASHI ET AL., J BIOCHEM (TOKYO), vol. 117, 1995, pages 452 - 7
J.J. KILLION; I.J. FIDLER, IMMUNOMETHODS, vol. 4, 1994, pages 273
JANINI ET AL., ELECTROPHORESIS, vol. 23, 2002, pages 1605 - 11
JONES, P. ET AL., NATURE, vol. 321, 1986, pages 522 - 525
K. KEINANEN; M.L. LAUKKANEN, FEBS LETT., vol. 346, 1994, pages 123
KABAT, E. A. ET AL.: "Sequences of Proteins of Immunological Interest", 1991, U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES
KARPOVSKY ET AL., J EXP. MED., vol. 160, 1984, pages 1686
KELLEY; O'CONNELL, BIOCHEM., vol. 32, 1993, pages 6862 - 35
KLIMKA ET AL., BRITISH J OF CANCER, vol. 83, no. 2, 2000, pages 252 - 260
KLINE ET AL., MOL. PHARMACEUT., vol. 1, 2004, pages 9 - 22
KOHLER; MILSTEIN, NATURE, vol. 256, 1975, pages 495
KOMISSAROV ET AL., J BIOL. CHEM., vol. 272, 1997, pages 26864 - 26870
KRATZ ET AL., BIOORG. MED. CHEM. LETT., vol. 11, 2001, pages 2001 - 2006
KRISHNAMURTHY R; MANNING MC, CURR PHARM BIOTECHNOL, vol. 3, 2002, pages 361 - 71
KURACHI; YAMAMOTO: "Handbook of Proeolytic Enzymes", vol. 2, 2004, pages: 1699 - 1702
KUROIWA ET AL., NATURE BIOTECHNOLOGY, vol. 20, 2002, pages 889 - 894
LEE, D. ET AL., BIOORGANIC AND MEDICINAL CHEMISTRY LETTERS, vol. 9, 1999, pages 1667 - 72
LEE, J BIOL. CHEM., vol. 275, pages 36720 - 36725
LEIBIGER ET AL., BIOCHEM J., vol. 338, 1999, pages 529 - 538
LEVI ET AL., PROC. NATL. ACAD. SCI. USA., vol. 90, 1993, pages 4374 - 8
LEVINE ET AL., COMP. BIOCHEM. PHYSIOL., vol. 72B, 1982, pages 77 - 85
LI ET AL., CANCER RES., vol. 42, 1982, pages 999
LIU ET AL., CANCER RES., vol. 60, 2000, pages 6061 - 6067
LIU, MA ET AL., PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 8648
LONBERG ET AL., NATURE, vol. 368, no. 6474, 1994, pages 856 - 859
LONBERG, N. ET AL., NATURE, vol. 368, no. 6474, 1994, pages 856 - 859
LONBERG, N.: "Handbook of Experimental Pharmacology", vol. 113, 1994, pages: 49 - 101
LONBERG, N.; HUSZAR, D., INTERN. REV. IMMUNOL., vol. 13, 1995, pages 65 - 93
M. OWAIS ET AL., ANTIMICROB. AGENTS CHEMOTHER., vol. 39, 1995, pages 180
MA ET AL., CHROMATOGRAPHIA, vol. 53, 2001, pages 75 - 89
MARCH J: "Advanced Organic Chemistry", 1992, JOHN WILEY AND SONS
MARCH: "Advanced Organic Chemistry", 1985, JOHN WILEY & SONS
MARSHALL ET AL., ANNU REV BIOCHEM, vol. 41, 1972, pages 673 - 702
MARTIN ET AL., J. ANTIBIOT., vol. 33, 1980, pages 902
MARTIN ET AL., J. ANTIBIOT., vol. 34, 1981, pages 1119
MATAYOSHI ET AL., SCIENCE, vol. 247, 1990, pages 954
MCCAFFERTY ET AL., NATURE, vol. 348, 1990, pages 552 - 554
MIMURA ET AL., MOL IMMUNOL, vol. 37, 2000, pages 697 - 706
MOLINO ET AL., JOURNAL OFBIOLOGICAL CHEMISTRY, vol. 272, no. 7, 1997, pages 4043 - 4049
MONTEIRO, R.C. ET AL., J IMMUNOL., vol. 148, 1992, pages 1764
MORRIS ET AL., PLANT MOLECULAR BIOLOGY, vol. 24, no. 673, 1994, pages 77
MORRISON, S., SCIENCE, vol. 229, 1985, pages 1202
MORTON, H.C. ET AL., CRITICAL REVIEWS IN IMMUNOLOGY, vol. 16, 1996, pages 423 - 440
MURRAY ET AL., J CHROMATOGR SCI, vol. 40, 2002, pages 343 - 9
NEEDLEMAN; WUNSCH, J MOL. BIOL., vol. 48, 1970, pages 444 - 453
NORD K ET AL.: "Binding proteins selected from combinatorial libraries of an a-helical bacterial receptor domain", NAT BIOTECHNOL, vol. 15, 1997, pages 772 - 7
P.G. BLOEMAN ET AL., FEBSLETT., vol. 357, 1995, pages 140
PAREKH ET AL., NATURE, vol. 316, 1985, pages 452 - 7
PASTAN, I.; KREITMAN, R. J., CURR. OPIN. INVESTIG. DRUGS, vol. 3, 2002, pages 1089 - 1091
PAULUS, BEHRING INS. MITT., no. 78, 1985, pages 118 - 132
PAYNE, G., CANCER CELL, vol. 3, 2003, pages 207 - 212
PETRACEK ET AL., ANNALS NYACAD SCI., vol. 507, 1987, pages 353 - 54
PEZZUTTO ET AL., JLMMUNOL., vol. 138, 1987, pages 2793
POLYMENIS; STOLLER, J IMMUNOL., vol. 152, 1994, pages 5218 - 5329
QUEEN, C. ET AL., PROC. NATL. ACAD. SEE. USA., vol. 86, 1989, pages 10029 - 10033
QUI ET AL., NATURE BIOTECHNOLOGY, vol. 25, no. 8, 2007, pages 921 - 929
R. J. KAUFMAN; P. A. SHARP, J MOL. BIOL., vol. 159, 1982, pages 601 - 621
RADER ET AL., PROC. NATL. ACAD. SCI. USA., vol. 95, 1998, pages 8910 - 8915
RANO, T.A. ET AL., CHEMISTRY AND BIOLOGY, vol. 4, 1997, pages 149 - 55
RIBATTI ET AL., INTERNATIONAL JOURNAL OF CANCER, vol. 85, no. 2, 2000, pages 171 - 5
RIECHMANN, L. ET AL., NATURE, vol. 332, 1998, pages 323 - 327
RONMARK J ET AL.: "Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A", EUR J BIOCHEM, vol. 269, 2002, pages 2647 - 55
RONMARK J; HANSSON M; NGUYEN T ET AL.: "Construction and characterization of affibody-Fc chimeras produced in Escherichia coli", J IMMUNOL METHODS, vol. 261, 2002, pages 199 - 211
SAITO, G. ET AL.: "Cytotoxic Compounds And Conjugates", ADV. DRUG DELIV. REV., vol. 55, 2003, pages 199 - 215
SANDLER SR; KARO W: "Organic Functional Group Preparations", 1983, ACADEMIC PRESS, INC.
SANDSTORM K ET AL.: "Inhibition of the CD28-CD80 co-stimulation signal by a CD28- binding Affibody ligand developed by combinatorial protein engineering", PROTEIN ENG, vol. 16, 2003, pages 691 - 7
SCHLOEMER ET AL., J. VIROLOGY, vol. 15, no. 4, 1975, pages 882 - 893
SCHREIER ET AL., J BIOL. CHEM., vol. 269, 1994, pages 9090
SEIDAH ET AL., METH. ENZYMOL., vol. 244, 1994, pages 175
SENTER, P.D.; SPRINGER, C.J., ADV. DRUG DELIV. REV., vol. 53, 2001, pages 247 - 264
SHEARWATER POLYMERS CATALOG, 2001
SHIELDS, R.L. ET AL., J BIOL. CHEM., vol. 276, 2001, pages 6591 - 6604
SHIELDS, R.L. ET AL., J BIOL. CHEM., vol. 277, 2002, pages 26733 - 26740
SMITH ET AL., METH. ENZYMOL., vol. 244, 1994, pages 412
SPIRO RG, GLYCOBIOLOGY, vol. 12, 2002, pages 43R - 56R
STACK ET AL., JOURNAL OFBIOLOGICAL CHEMISTRY, vol. 269, no. 13, 1994, pages 9416 - 9419
SWENSON ET AL., CANCER RES., vol. 42, 1982, pages 2821
TAKANAMI ET AL., CANCER, vol. 88, no. 12, 2000, pages 2686 - 92
TAKEBE, Y. ET AL., MOL. CELL. BIOL., vol. 8, 1988, pages 466 - 472
TAM ET AL., AM. J RESPIR. CELL MOL. BIOL., vol. 3, 1990, pages 27 - 32
TARENTINO, A.L. ET AL., BIOCHEM., vol. 14, 1975, pages 5516 - 23
TAYLOR, L. ET AL., INTERNATIONAL IMMUNOLOGY, vol. 6, 1994, pages 579 - 591
TAYLOR, L. ET AL., NUCLEIC ACIDS RESEARCH, vol. 20, 1992, pages 6287 - 6295
TEDDER ET AL., CURR DIR AUTOIMMUN, vol. 8, 2005, pages 55
TEDDER ET AL., IMMUNOL TODAY, vol. 15, 1994, pages 437
THORNBERRY, METH. ENZYMOL., vol. 244, 1994, pages 615
TOMIZUKA ET AL., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 722 - 727
TOMLINSON, I. M. ET AL.: "The Repertoire of Human Germline VH Sequences Reveals about Fifty Groups of VH Segments with Different Hypervariable Loops", J MOL. BIOL., vol. 227, 1992, pages 776 - 798
TOTH-JAKATICS ET AL., HUMAN PATHOLOGY, vol. 31, no. 8, 2000, pages 955 - 960
TRAIL, P.A. ET AL., CANCER IMMUNOL. IMMUNOTHER., vol. 52, 2003, pages 328 - 337
TUAILLON ET AL., J IMMUNOL., vol. 152, 1994, pages 2912 - 2920
TUAILLON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 3720 - 3724
UMANA ET AL., NAT. BIOTECH., vol. 17, 1999, pages 176 - 180
UMEZAWA ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 153, 1988, pages 1038
URLAUB; CHASIN, PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216 - 4220
V.V. RANADE, J CLIN. PHARMACOL., vol. 29, 1989, pages 685
WADE LG: "Compendium of Organic Synthetic Methods", 1980, JOHN WILEY AND SONS
WALLICK ET AL., JEXP MED, vol. 168, 1988, pages 1099 - 109
WARD ET AL., NATURE, vol. 341, 1989, pages 544 - 546
WARD ET AL., PHOTOCHEM. PHOTOBIOL., vol. 35, 1982, pages 803 - 808
WEBER ET AL., METH. ENZYMOL., vol. 244, 1994, pages 595
WILBANKS ET AL., J BIOL. CHEM., vol. 268, 1993, pages 1226 - 35
XU; DAVIS, IMMUNITY, vol. 13, 2000, pages 37 - 45
YAMANE-OHNUKI ET AL., BIOTECHNOL BIOENG, vol. 87, 2004, pages 614 - 22
ZIMMERMAN, M. ET AL., ANALYTICAL BIOCHEMISTRY, vol. 78, 1977, pages 47 - 51

Cited By (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3597216A1 (en) 2008-08-11 2020-01-22 E. R. Squibb & Sons, L.L.C. Human antibodies that bind lymphocyte activation gene-3 (lag-3) and uses thereof
EP2905030A1 (en) 2008-08-11 2015-08-12 E. R. Squibb & Sons, L.L.C. Human antibodies that bind lymphocyte activation gene-3 (LAG-3) and uses thereof
EP4147714A1 (en) 2008-08-11 2023-03-15 E. R. Squibb & Sons, L.L.C. Human antibodies that bind lymphocyte activation gene-3 (lag-3) and uses thereof
EP2409712A1 (en) 2010-07-19 2012-01-25 International-Drug-Development-Biotech Anti-CD19 antibody having ADCC and CDC functions and improved glycosylation profile
EP2409993A1 (en) 2010-07-19 2012-01-25 International-Drug-Development-Biotech Anti-CD19 antibody having ADCC function with improved glycosylation profile
WO2012010562A1 (en) 2010-07-19 2012-01-26 International - Drug - Development - Biotech Anti-cd19 antibody having adcc and cdc functions and improved glycosylation profile
WO2012010561A1 (en) 2010-07-19 2012-01-26 International - Drug - Development - Biotech Anti-cd19 antibody having adcc function with improved glycosylation profile
WO2014008218A1 (en) 2012-07-02 2014-01-09 Bristol-Myers Squibb Company Optimization of antibodies that bind lymphocyte activation gene-3 (lag-3), and uses thereof
EP3275899A1 (en) 2012-07-02 2018-01-31 Bristol-Myers Squibb Company Optimization of antibodies that bind lymphocyte activation gene-3 (lag-3), and uses thereof
EP3795592A1 (en) 2012-07-02 2021-03-24 Bristol-Myers Squibb Company Optimization of antibodies that bind lymphocyte activation gene-3 (lag-3), and uses thereof
US11059910B2 (en) 2012-12-03 2021-07-13 Novimmune Sa Anti-CD47 antibodies and methods of use thereof
US11840553B2 (en) 2012-12-03 2023-12-12 Novimmune Sa Anti-CD47 antibodies and methods of use thereof
US10980890B2 (en) 2014-05-28 2021-04-20 Legochem Biosciences, Inc. Compounds comprising self-immolative group
US11236161B2 (en) 2014-06-02 2022-02-01 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Chimeric antigen receptors targeting CD-19
WO2015187835A2 (en) 2014-06-06 2015-12-10 Bristol-Myers Squibb Company Antibodies against glucocorticoid-induced tumor necrosis factor receptor (gitr) and uses thereof
EP3998079A1 (en) 2014-06-06 2022-05-18 Bristol-Myers Squibb Company Antibodies against glucocorticoid-induced tumor necrosis factor receptor (gitr) and uses thereof
EP3610924A1 (en) 2014-06-06 2020-02-19 Bristol-Myers Squibb Company Antibodies against glucocorticoid-induced tumor necrosis factor receptor (gitr) and uses thereof
EP3805267A1 (en) * 2014-08-28 2021-04-14 Juno Therapeutics, Inc. Antibodies and chimeric antigen receptors specific for cd19
TWI805109B (en) * 2014-08-28 2023-06-11 美商奇諾治療有限公司 Antibodies and chimeric antigen receptors specific for cd19
TWI751102B (en) * 2014-08-28 2022-01-01 美商奇諾治療有限公司 Antibodies and chimeric antigen receptors specific for cd19
IL250831B2 (en) * 2014-08-28 2023-11-01 Juno Therapeutics Inc Antibodies and chimeric antigen receptors specific for cd19, compositions containing same and uses thereof
IL250831B1 (en) * 2014-08-28 2023-07-01 Juno Therapeutics Inc Antibodies and chimeric antigen receptors specific for cd19, compositions containing same and uses thereof
WO2016033570A1 (en) * 2014-08-28 2016-03-03 Juno Therapeutics, Inc. Antibodies and chimeric antigen receptors specific for cd19
RU2741105C2 (en) * 2014-08-28 2021-01-22 Джуно Терапьютикс, Инк. Antibodies and chimeric antigenic receptors specific to cd19
US10533055B2 (en) 2014-08-28 2020-01-14 Juno Therapeutics, Inc. Antibodies and chimeric antigen receptors specific for CD19
US11827714B2 (en) 2014-08-28 2023-11-28 Juno Therapeutics, Inc. Antibodies and chimeric antigen receptors specific for CD19
EP3789399A1 (en) 2014-11-21 2021-03-10 Bristol-Myers Squibb Company Antibodies comprising modified heavy constant regions
EP3725808A1 (en) 2014-11-21 2020-10-21 Bristol-Myers Squibb Company Antibodies against cd73 and uses thereof
WO2016081748A2 (en) 2014-11-21 2016-05-26 Bristol-Myers Squibb Company Antibodies against cd73 and uses thereof
EP4249066A2 (en) 2014-12-23 2023-09-27 Bristol-Myers Squibb Company Antibodies to tigit
US11286300B2 (en) 2015-10-01 2022-03-29 Hoffmann-La Roche Inc. Humanized anti-human CD19 antibodies and methods of use
WO2017087678A2 (en) 2015-11-19 2017-05-26 Bristol-Myers Squibb Company Antibodies against glucocorticoid-induced tumor necrosis factor receptor (gitr) and uses thereof
US11173214B2 (en) 2015-11-25 2021-11-16 Legochem Biosciences, Inc. Antibody-drug conjugates comprising branched linkers and methods related thereto
US11167040B2 (en) 2015-11-25 2021-11-09 Legochem Biosciences, Inc. Conjugates comprising peptide groups and methods related thereto
US11413353B2 (en) 2015-11-25 2022-08-16 Legochem Biosciences, Inc. Conjugates comprising self-immolative groups and methods related thereto
US11975076B2 (en) 2015-11-25 2024-05-07 Legochem Biosciences, Inc. Antibody-drug conjugates comprising branched linkers and methods related thereto
WO2017152085A1 (en) 2016-03-04 2017-09-08 Bristol-Myers Squibb Company Combination therapy with anti-cd73 antibodies
WO2018083535A1 (en) * 2016-11-04 2018-05-11 Novimmune Sa Anti-cd19 antibodies and methods of use thereof
US11142570B2 (en) 2017-02-17 2021-10-12 Bristol-Myers Squibb Company Antibodies to alpha-synuclein and uses thereof
WO2018151821A1 (en) 2017-02-17 2018-08-23 Bristol-Myers Squibb Company Antibodies to alpha-synuclein and uses thereof
US11827695B2 (en) 2017-02-17 2023-11-28 Bristol-Myers Squibb Company Antibodies to alpha-synuclein and uses thereof
US11654197B2 (en) 2017-03-29 2023-05-23 Legochem Biosciences, Inc. Pyrrolobenzodiazepine dimer prodrug and ligand-linker conjugate compound of the same
WO2018213297A1 (en) 2017-05-16 2018-11-22 Bristol-Myers Squibb Company Treatment of cancer with anti-gitr agonist antibodies
EP4098662A1 (en) 2017-05-25 2022-12-07 Bristol-Myers Squibb Company Antibodies comprising modified heavy constant regions
WO2018218056A1 (en) 2017-05-25 2018-11-29 Birstol-Myers Squibb Company Antibodies comprising modified heavy constant regions
TWI812645B (en) * 2017-09-21 2023-08-21 中國大陸商上海藥明生物技術有限公司 Novel Anti-CD19 Antibody
EP3684820A4 (en) * 2017-09-21 2021-05-26 Wuxi Biologics (Cayman) Inc. Novel anti-cd19 antibodies
US11497769B2 (en) 2017-09-21 2022-11-15 WuXi Biologics Ireland Limited Anti-CD19 antibodies
WO2019215510A3 (en) * 2018-05-09 2020-01-09 Legochem Biosciences, Inc. Compositions and methods related to anti-cd19 antibody drug conjugates
RU2806333C2 (en) * 2018-05-09 2023-10-31 Легокем Байосайенсиз, Инк. Compositions and methods relating to drug conjugates with anti-cd19 antibodies
US11827703B2 (en) 2018-05-09 2023-11-28 Legochem Biosciences, Inc. Compositions and methods related to anti-CD19 antibody drug conjugates
WO2019243626A1 (en) 2018-06-22 2019-12-26 Genmab A/S Method for producing a controlled mixture of two or more different antibodies
US11793834B2 (en) 2018-12-12 2023-10-24 Kite Pharma, Inc. Chimeric antigen and T cell receptors and methods of use
WO2020154889A1 (en) * 2019-01-29 2020-08-06 上海鑫湾生物科技有限公司 Combination of antibody having fc mutant and effector cell, use thereof and preparation method therefor
CN113840841B (en) * 2019-05-20 2023-03-07 南京驯鹿医疗技术有限公司 Fully human antibody targeting CD19 and application thereof
CN113840841A (en) * 2019-05-20 2021-12-24 南京驯鹿医疗技术有限公司 Fully human antibody targeting CD19 and application thereof
US11707533B2 (en) 2019-09-04 2023-07-25 Legochem Biosciences, Inc. Antibody-drug conjugate comprising antibody against human ROR1 and use for the same
WO2021067598A1 (en) 2019-10-04 2021-04-08 Ultragenyx Pharmaceutical Inc. Methods for improved therapeutic use of recombinant aav
US11299551B2 (en) 2020-02-26 2022-04-12 Biograph 55, Inc. Composite binding molecules targeting immunosuppressive B cells
WO2023198744A1 (en) 2022-04-13 2023-10-19 Tessa Therapeutics Ltd. Therapeutic t cell product
WO2023245106A1 (en) * 2022-06-16 2023-12-21 Abbvie Biotherapeutics Inc. Anti-cd19 antibody drug conjugates
US12121589B2 (en) 2023-06-15 2024-10-22 Abbvie Biotherapeutics Inc. Anti-CD19 antibody drug conjugates

Also Published As

Publication number Publication date
JP2010513303A (en) 2010-04-30
WO2009054863A3 (en) 2009-09-24
BRPI0718349A2 (en) 2014-01-14
CA2672800A1 (en) 2009-04-30
EP2101817A2 (en) 2009-09-23
AU2007360636A1 (en) 2009-04-30
EP2101817A4 (en) 2013-05-01
AR064337A1 (en) 2009-04-01
TW200833713A (en) 2008-08-16
US20100104509A1 (en) 2010-04-29
MX2009006275A (en) 2009-07-22
CN101636502A (en) 2010-01-27
KR20090088940A (en) 2009-08-20
JP5517626B2 (en) 2014-06-11
CN101636502B (en) 2014-06-04
CL2007003622A1 (en) 2009-08-07

Similar Documents

Publication Publication Date Title
JP5517626B2 (en) Human antibodies that bind to CD19 and uses thereof
US9499632B2 (en) Human antibodies that bind CD22 and uses thereof
AU2008308956B2 (en) Human antibodies that bind mesothelin, and uses thereof
US20100150950A1 (en) Human antibodies that bind cd70 and uses thereof
AU2008334063A1 (en) Anti-B7H4 monoclonal antibody-drug conjugate and methods of use
EP2229187A2 (en) Monoclonal antibody partner molecule conjugates directed to protein tyrosine kinase 7 (ptk7)
SG173742A1 (en) Fully human antibodies specific to cadm1
AU2007329529B2 (en) Human antibodies that bind CD22 and uses thereof

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 578353

Country of ref document: NZ

Ref document number: 200780050552.1

Country of ref document: CN

ENP Entry into the national phase

Ref document number: 2009541583

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2672800

Country of ref document: CA

Ref document number: MX/A/2009/006275

Country of ref document: MX

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2007360636

Country of ref document: AU

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07875235

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2007875235

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2460/KOLNP/2009

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 1020097014232

Country of ref document: KR

ENP Entry into the national phase

Ref document number: 2007360636

Country of ref document: AU

Date of ref document: 20071213

Kind code of ref document: A

REG Reference to national code

Ref country code: BR

Ref legal event code: B01E

Ref document number: PI0718349

Country of ref document: BR

Free format text: SOLICITA-SE A REGULARIZACAO DA PROCURACAO, UMA VEZ QUE A PROCURACAO APRESENTADA NAO POSSUI DATA.

ENP Entry into the national phase

Ref document number: PI0718349

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20090612