WO2009028644A1 - Probe and primer for detection of obligate anaerobic bacteria - Google Patents

Probe and primer for detection of obligate anaerobic bacteria Download PDF

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Publication number
WO2009028644A1
WO2009028644A1 PCT/JP2008/065495 JP2008065495W WO2009028644A1 WO 2009028644 A1 WO2009028644 A1 WO 2009028644A1 JP 2008065495 W JP2008065495 W JP 2008065495W WO 2009028644 A1 WO2009028644 A1 WO 2009028644A1
Authority
WO
WIPO (PCT)
Prior art keywords
probe
primer
detection
obligate anaerobic
anaerobic bacteria
Prior art date
Application number
PCT/JP2008/065495
Other languages
French (fr)
Japanese (ja)
Inventor
Nobuyuki Hayashi
Original Assignee
Kirin Beer Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kirin Beer Kabushiki Kaisha filed Critical Kirin Beer Kabushiki Kaisha
Priority to JP2009530193A priority Critical patent/JPWO2009028644A1/en
Publication of WO2009028644A1 publication Critical patent/WO2009028644A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

The object is to provide a probe and a primer both of which enable to identify an obligate anaerobic bacterium accurately, rapidly and simply. Thus, disclosed is a LAMP primer set for use in the detection of an obligate anaerobic bacterium, which comprises primers each selected from the group consisting of polynucleotides respectively represented by the nucleotide sequences depicted in SEQ ID NOs: 1 to 5, 7 to 11, 13 to 16, 18 to 22 and 24 to 27 and polynucleotides homologous with the aforementioned polynucleotides.
PCT/JP2008/065495 2007-08-30 2008-08-29 Probe and primer for detection of obligate anaerobic bacteria WO2009028644A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009530193A JPWO2009028644A1 (en) 2007-08-30 2008-08-29 Probes and primers for detection of obligate anaerobic bacteria

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007223783 2007-08-30
JP2007-223783 2007-08-30

Publications (1)

Publication Number Publication Date
WO2009028644A1 true WO2009028644A1 (en) 2009-03-05

Family

ID=40387355

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/065495 WO2009028644A1 (en) 2007-08-30 2008-08-29 Probe and primer for detection of obligate anaerobic bacteria

Country Status (2)

Country Link
JP (1) JPWO2009028644A1 (en)
WO (1) WO2009028644A1 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003250557A (en) * 2002-02-28 2003-09-09 Sapporo Breweries Ltd Method for discriminating of beer by lactobacillus brevis by using gyrase gene
WO2005093059A1 (en) * 2004-03-25 2005-10-06 Sapporo Breweries Limited Primer and primer set for detecting and discriminating yeast, lactic acid bacterium and obligate anaerobe and detection/discrimination method using the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003250557A (en) * 2002-02-28 2003-09-09 Sapporo Breweries Ltd Method for discriminating of beer by lactobacillus brevis by using gyrase gene
WO2005093059A1 (en) * 2004-03-25 2005-10-06 Sapporo Breweries Limited Primer and primer set for detecting and discriminating yeast, lactic acid bacterium and obligate anaerobe and detection/discrimination method using the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TOMOO OGATA: "Beer jozo kankei biseibutsu no atarashii keitogakuteki chiken to kore o riyo shita yugai biseibutsu kenshutsuho", JOURNAL OF THE BREWING SOCIETY OF JAPAN, vol. 95, 2000, pages 803 - 810 *
YAMAMOTO S. & HARAYAMA S.: "PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains", APPL.ENVIRON.MICROBIOL., vol. 61, 1995, pages 1104 - 1109 *

Also Published As

Publication number Publication date
JPWO2009028644A1 (en) 2010-12-02

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