WO2009024998A1 - Nitric oxide releasing derivatives of paracetamol - Google Patents

Nitric oxide releasing derivatives of paracetamol Download PDF

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WO2009024998A1
WO2009024998A1 PCT/IN2008/000514 IN2008000514W WO2009024998A1 WO 2009024998 A1 WO2009024998 A1 WO 2009024998A1 IN 2008000514 W IN2008000514 W IN 2008000514W WO 2009024998 A1 WO2009024998 A1 WO 2009024998A1
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paracetamol
acetamide
compounds
activity
compound according
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PCT/IN2008/000514
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French (fr)
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Tilak Raj Bhardwaj
Manoj Kumar
Neeraj Mehta
Neelima Dhingra
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Council Of Scientific & Industrial Research
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Priority to ES08789930.8T priority Critical patent/ES2441263T3/en
Priority to EP08789930.8A priority patent/EP2188245B1/en
Publication of WO2009024998A1 publication Critical patent/WO2009024998A1/en
Priority to US12/707,410 priority patent/US8207222B2/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/01—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C233/16—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms
    • C07C233/24—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by a carbon atom of a six-membered aromatic ring
    • C07C233/25—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by a carbon atom of a six-membered aromatic ring having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]

Definitions

  • the present invention relates to nitric oxide releasing derivatives of paracetamol.
  • the present invention particularly relates to novel nitrate esters of paracetamol.
  • the present invention pertains to process of preparation of nitrate esters of paracetamol. More particularly, the invention relates to nitric oxide releasing derivatives of paracetamol which show better analgesic and anti-inflammatory activities and decreased liver toxicities as apparent from their biochemical and histopathologic profile.
  • Paracetamol (paracetamol, 1) is among most commonly used analgesics. It effectively reduces fever and mild-to moderate pain, and is regarded, in general, as a very safe drug. Nevertheless, overdose (deliberate) is a common cause of hepatic injury, accounting for ⁇ 40% of cases of acute liver failure in the USA.
  • overdose (deliberate) is a common cause of hepatic injury, accounting for ⁇ 40% of cases of acute liver failure in the USA.
  • NCX-701 (3) by adding NO moiety to paracetamol, via an aliphatic spacer to improve the anti-inflammatory activity of paracetamol, based on the well-demonstrated activity of NO on cytokine synthesis and regulation of proinflammatory mediators.
  • NO may modulate spinal and sensory neuron excitability through multiple mechanisms that may improve the antinociceptive activity of paracetamol.
  • the pharmacological profile of NCX 701 has been reviewed. [ Marshall, M.; Moore, P. K. Effect of nitric oxide releasing paracetamol and flurbiprofen on cytokine production in human blood. Eur. J. Pharmacol, 2004, 483, 317-322; and al-Swayeh, O. A.; Futter, L. E.; Clifford, R. H.; Moore, P. K. Nitroparacetamol exhibits anti-inflammatory and anti-nociceptive activity. Br. J. Pharmacol, 2000, 130, 1453-1456].
  • paracetamol Compared with NSAIDs, paracetamol possesses little anti-inflammatory activity in humans and it does not induce damage of the gastric mucosa, but it could cause liver damage (or even poisoning) when used in high dosage.
  • the aim of coupling paracetamol with NO has, therefore, been to improve its anti-inflammatory activity, and also to exploit the cytoprotective properties of NO to reduce potential liver damage.
  • Concerning safety, several studies have highlighted the liver sparing profile of NCX 701 compared to paracetamol.
  • the beneficial protective effect on the liver has been supposed to be related to the fact that NO can reduce the synthesis of several proinflammatory cytokines. [Wallace, J. L. Paracetamol hepatotoxicity: NO to the rescue. Br. J. Pharmacol, 2004, 143, 1-2].
  • NCX-701 Nitroparacetamol
  • NCX 701 provides similar analgesic efficacy to paracetamol Ig in post-operative dental pain, therefore with a reduced exposure to paracetamol, confirming a contribution of NO to the anti-nociceptive mechanisms of NCX 701.
  • O-Dealkylation of a homologous series of alkoxy acetanilides [p-methoxy, p-ethoxy: phenacetin, p-(n)-propoxy- and p-(n)-butoxy acetanilides; 4-7] have shown to release paracetamol.
  • the compounds (6 and 7) were structurally related to phenacetin (5), and were synthesized to check whether they release paracetamol (1) upon 0-dealkylation, which they did.
  • the chain length in the synthesized compounds was varied to study the effect of chain lengthening on the rate of release of paracetamol.
  • the main object of the present invention is to provide novel nitrate esters of paracetamol.
  • Another object of the present invention is to provide process for preparation of novel nitrate esters of paracetamol.
  • Yet another object of the present invention is to develop nitric oxide releasing derivative of paracetamol that shows better analgesic, anti-inflammatory, biochemical (SGOT, ALP levels) and histopathologic profile. Further object of the present invention is to develop a pharmaceutical composition comprising nitric oxide releasing derivatives of paracetamol.
  • Still another object of the present invention is to inhibit COX-2 in vitro and release NO in vivo.
  • Compound, (13) has shown to have better COX-2 (in vitro) inhibition activity i.e. 32.5% than the parent drug paracetamol (1) which had 24.0%.
  • the compounds 12 and 13 also released NO in vivo.
  • the present invention relates to the synthesis of NO-releasing derivatives of paracetamol and their evaluation.
  • the compounds showed better analgesic, anti-inflammatory, biochemical and histopathologic profile.
  • the present invention particularly relates to novel nitrate esters of paracetamol.
  • the nitrate esters of paracetamol are prepared by reacting the paracetamol with dihaloalkyl compound and followed by reaction with silver nitrate to obtain the corresponding nitrate ester derivatives.
  • FIG 1 Analgesic activity of paracetamol (1) and NO-NSAIDs (12 and 13)
  • SGPT SGPT
  • ALT alanine aminotransferase
  • the graph indicates that the serum NO concentration 6 hours after administration of 12 and 13 was greater than the control (0.9% w/v sodium chloride containing 20% v/v tween-80) confirming that these compounds release NO in vivo.
  • FIG. 5 Histopathology (haematoxylin and eosin) of liver samples
  • the present invention provides nitrate ester derivatives of paracetamol of general formula A, wherein the value of n is selected from 2 to 5.
  • the compound of formula A is having better peripheral 'analgesic activity and significant anti-inflammatory activity as compared to paracetamol.
  • the compounds 12 and 13 showed increase in NO serum level 354.2 ⁇ 2.17 and 265.4 ⁇ 2.47 respectively w.r.t. control (182.1 ⁇ 0.68) at a dose of 100mg/kg body weight.
  • the compound 13 showed in vitro cox-2 (ovine) inhibitory activity 32.5% as compared to control 24.0%.
  • the present invention provides a process for preparation of nitrate ester derivative of paracetamol comprising the steps: a) reacting paracetamol with dibromo alkane in organic solvent in presence of alkali carbonate for a period up to 12 hours, b) filtering the reaction mixture followed by evaporation of solvent to obtain solid residue, c) dissolving the residue in water immisible solvent and washing with sodium hydroxide solution followed by washing with water,evaprating the solvent to O-alkylated bromo derivative of paracetamol, d) reacting the O-alkylated bromo derivative of paracetamol with sliver nitrate under reflux in an aprotic solvent upto 12 hours, further filtering and evaporating the f ⁇ lterate to obtain the nitrate esters derivative of Paracetamol of general formula A wherein the value of n is selected from 2-5.
  • dihalo alkane may be selected from a group consisting of 1,2-dibromoethane, 1,3-dibromopropane, 1,4-dibromobutane and 1,5- dibromopentane.
  • the organic solvent used may be selected from a group consisting of ethyl methyl ketone, acetone, acetonitrile and mixture thereof.
  • aprotic solvent used may be selected from a group consisting of acetonitrile, benzene, hexane and mixture thereof.
  • the pharmaceutical composition may be prepared using the compounds of the general formula A along with the pharmaceutically acceptable excipients.
  • Compounds 12 and 13 are novel and have not been reported earlier.
  • Compound (13) has shown to have better COX-2 inhibition activity (in vitro) than the parent drug paracetamol.
  • the data of analgesic and anti-inflammatory activities is exactly complimenting and supporting it. Therefore, it can be concluded that the NO releasing compound is showing better activity than the parent drug candidate, and moreover, the biochemical and histopathological studies carried out, indicate that the compound is causing less liver toxicity than paracetamol.
  • NO-releasing derivatives of paracetamol, (12) and (13) showed good anti-inflammatory activity w.r.t. parent drug, both at 2 hr and 4 hr interval. No significant activity was observed in case of paracetamol (1).
  • O-alkylated NO-releasing derivatives (12 and 13) of paracetamol (1) exhibited better response than the parent drug indicated by greater % inhibition in writings.
  • the intermediates 10 and 11, having terminal Br (which was later converted to -ONO 2 moiety on treatment with AgNO 3 ) showed very negligible % inhibition in writings indicating that NO release is responsible for better analgesic activity of the compounds 12 and 13 (having NO releasing -ONO 2 ) moiety. In NO releasing activity they also showed significant increase in level of NO.
  • Paracetamol (1) a proven liver toxic and having no anti-inflammatory activity of its own has been converted to more active and lesser toxic NO releasing compounds 12 and 13.
  • Nitric oxide releasing derivatives of paracetamol are developed (12 and 13) ( Figure 2), which have shown better analgesic, anti-inflammatory, biochemical (SGOT, ALP levels) and histopathologic profile. Besides these derivatives have shown to inhibit COX-2 in vitro and release NO in vivo.
  • the liver toxic drug paracetamol (1) having no anti-inflammatory activity has been converted to less liver toxic and more active derivatives. Briefly, Paracetamol was stirred in a solution of 1, 2-dibromoethane (8) and 1, 5-dibromopentane (9), in ethyl methyl ketone and K 2 CO 3 for 8-10 hours. The resulting solution was filtered, dried and evaporated to get a solid residue.
  • mice Wistar rats (male 150-20Og) of both sex and Laca mice (male, 25-35g) procured from Central Animal House, Punjab University, Chandigarh, India were used. Animals were housed under standard laboratory conditions and maintained on rat chow. Animals were allowed free access to food and water until used and fasted 24 hr prior to studies.
  • test compounds were suspended in 0.5% carboxymethylcellulose (CMC) and administered per orally (p.o.). Control animals were given the corresponding amount of vehicle (0.5%, CMC).
  • CMC carboxymethylcellulose
  • Anti-inflammatory activity was determined by using carrageenan induced rat paw edema model. Rats were divided into different groups and the drugs were administered to each group. The paracetamol (1) and synthesized NO- releasing derivatives (12 and 13) were administered p.o. at a dose of 100 mg/kg, p.o., emulsified in 0.5% sodium carboxy methyl cellulose (0.5% sodium CMC). Anti- inflammatory activity was determined by using carrageenan induced rat paw edema model. Rats were divided into different groups and the drugs/derivatives were administered to each group.
  • Acute edema was induced in left hind paw of rats by injecting freshly prepared solution of carrageenan (Type IV, 0.1ml, 1%) under plantar region of left hind paw. In the right paw, saline (ImI, 0.9%) was injected, which served as control for comparison.
  • the increase in paw volume was measured by using plethysmometer (water displacement, UGO BASILE, Varese, Italy) at 2 hr and 4 hr after carrageenan challenge. Percentage change (increase) in paw volume was calculated and expressed as the amount of inflammation.
  • V r Volume of right paw (control)
  • Results are expressed as mean ⁇ standard error of mean (SEM).
  • NO-releasing derivatives of paracetamol (1), 12 and 13 showed good anti-inflammatory activity w.r.t. parent drug 1, both at 2 hr and 4 hr interval (shown by less % increase in paw volume).
  • At 2 hr % increase in paw volume for 12 and 13 was 32.68 ⁇ 1.45 and 31.15 ⁇ 1.31 respectively, and at 4 hr % increase in paw volume for 12 and 13 was 26.44 ⁇ 0.10 and 28.45 ⁇ 1.28 respectively.
  • Analgesic activity (Table 2 and FIG. 1 Sheet No. 1 Figure 3): Analgesic activity was determined by using abdominal writhing assay. Mice were divided into different groups containing 6 animals in each group. Analgesic activity was determined against acetic acid induced writhing assay. Writhing was induced by intraperitoneal (i.p.) injection of freshly prepared acetic acid solution. Mice were per orally (p.o.) administered paracetamol (1) and synthesized NO-releasing derivatives (12 and 13) at a dose of 100 mg/kg, emulsified in 0.5% sodium carboxy methyl cellulose (sodium CMC) vehicle, 30 min prior to i.p.
  • acetic acid 1% w/v in saline pH 2.7, 10 ml/kg, i.p.
  • Animals were immediately transferred to individual observation chambers and number of writhes (constriction of abdomen, turning of trunk, and extension of hind limbs) were monitored over the following 20 minutes, beginning 3 min after the injection of acetic acid. At the end of observation period the animals were killed by cervical dislocation and exsaguination. The average number of writhes in each group of drug treated mice was compared with that of the control group and degree of analgesia was expressed as % inhibition calculated from the equation :
  • N t number of writhes in drug treated mice
  • Table 1 Analgesic of paracetamol (1) and NO-NSAIDs (12 and 13) Results are expressed as mean ⁇ standard error of mean (SEM). # indicates P ⁇ 0.05 w.r.t. Paracetamol (100 mg/kg p.o.)
  • Oalkylated NO-releasing derivatives 12 and 13 of paracetamol (1) exhibited better response than the parent drug indicated by greater % inhibition in writings.
  • the % inhibition in case of 12 and 13 (58.35 ⁇ 1.50, 61.80 ⁇ 1.69 respectively) was found to be better than paracetamol (1) which was 45.36 ⁇ 1.34.
  • the intermediates 10 and 11, having terminal Br (which was later converted to -ONO 2 moiety on treatment with AgNO 3 ) showed very negligible % inhibition in writings indicating that NO release is responsible for better analgesic activity of the compounds 12 and 13 (having NO releasing -ONO 2 ) moiety.
  • Biochemical Assays (Table 3 and FIG. 2 Sheet No. 1 for SGPT(ALT), and Table 4 and FIG. 3 Sheet No.2 for ALP): Paracetamol was given at a dose of 1000 mg/Kg and an equivalent dose of 1000 mg/Kg of the derivatives 12 and 13 was given p.o. Before administration blood samples of each animal were taken and after 6 hrs the animals were sacrificed to collect blood samples, and the serum was separated out. Measurement of plasma markers of liver Serum Glutamate oxaloacetate transaminase (SGOT) pyruvate transaminase (SGPT) and Alkaline Phosphatase (ALP) were assayed spectrophotometrically using commercially available kits.
  • SGOT Serum Glutamate oxaloacetate transaminase
  • SGPT pyruvate transaminase
  • ALP Alkaline Phosphatase
  • mice were divided into different groups containing 6-8 animals in each group. Animals were lightly anaesthetized with diethyl ether and blood samples were collected which served as the basal reading of the enzyme to be analyzed. These groups of rats were then treated with paracetamol (1000 mg/Kg, Lp.), a ⁇ d equivalent dose of the test compounds. Control group was treated with an equal volume of vehicle (0.9% w/v sodium chloride containing 20% v/v tween-80). Animals were lightly anesthetized with ethyl ether and blood samples were collected and allowed to coagulate at room temperature for 1 hr, serum was then separated by centrifugation (5,000 rpm for 10 min) at 4°C and stored at -
  • Plasma markers of liver injury were assayed spectrophotometrically using following commercially available kits according to standard laboratory techniques:
  • Results are expressed as mean ⁇ standard error of mean (SEM).
  • Results are expressed as mean ⁇ standard error of mean (SEM).
  • liver histopathology For liver histopathology, the livers were removed and portions were fixed in 10% buffered formalin (pH 7.4). They were then processed by standard histopathological techniques, stained with Haematoxylin and Eosin (H&E), and examineed for morphologic evaluation for liver injury. 21
  • Results are expressed as mean ⁇ standard error of mean (SEM).
  • NO-releasing derivatives showed increase in % NO serum level w.r.t. control.
  • the NO release of these compounds could be the acting force behind their better analgesic and anti-inflammatory activities than the parent drug.
  • In vitro cox-2 (ovine) inhibitory assay The prostaglandin product is quantified via enzyme immunoassay (EIA) using a broadly specific antibody that binds to all major prostaglandin compounds. Assay kit was purchased from Cayman Chemical Company, USA.
  • Celecoxib 79.0% (positive control)
  • the NO-releasing should have been found to have better peripheral analgesic activity and significant anti-inflammatory activity as compared to the parent drug.
  • the derivatives were found to release NO in vivo and are devoid of liver toxicity as indicated by serum lesser increase in serum GPT(ALT) and ALP levels and histopathological studies. Moreover, 13 showed better COX-2 (ovine) binding activity than the parent drug. On the whole more effective and less toxic derivatives of 1 have been synthesised.
  • NO releasing compounds have shown better analgesic and anti inflammatory activity than the parent drug, and less liver toxicity in biochemical and histopathological studies.

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Abstract

The present invention particularly relates to novel nitrate esters of paracetamol. The nitrate esters of paracetamol are prepared by reacting the paracetamol with dihaloalkyl compound and followed by reaction with silver nitrate to obtain the corresponding nitrate ester derivatives. The nitrate esters of paracetamol are useful as analgesic, anti-inflammatory agents.

Description

"NITRIC OXIDE RELEASING DERIVATIVES OF PARACETAMOL"
FIELD OF THE INVENTION
The present invention relates to nitric oxide releasing derivatives of paracetamol. The present invention particularly relates to novel nitrate esters of paracetamol. The present invention pertains to process of preparation of nitrate esters of paracetamol. More particularly, the invention relates to nitric oxide releasing derivatives of paracetamol which show better analgesic and anti-inflammatory activities and decreased liver toxicities as apparent from their biochemical and histopathologic profile.
BACKGROUND OF THE INVENTION
Paracetamol (paracetamol, 1) is among most commonly used analgesics. It effectively reduces fever and mild-to moderate pain, and is regarded, in general, as a very safe drug. Nevertheless, overdose (deliberate) is a common cause of hepatic injury, accounting for ~ 40% of cases of acute liver failure in the USA. [Wu, C. C. Nitric oxide and inflammation. Curr. Med. Chem.- Anti-inflammatory & anti-allergy agents, 2004, 3, 217-222; Joshi, G. P. NCX-701. NicOx. Curr. Opin. Investig Drugs, 2004, 5, 755-759; and Moore, P. K.; Marshall, M. Nitric oxide releasing paracetamol (nitroparacetamol). Dig Liver Dis., 2003, 35.Ji1It is an effective analgesic and antipyretic agent and unlike other NSAIDs (Nonsteroidal Anti-inflammatory Drugs) has been reported to have little anti-inflammatory effects. [ Fiorucci, S.; Antonelli, E.; Mencarelli, A.; Palazzetti, B.; Alvarez-Miller, L.; Muscara, M.; del Soldato, P.; Sanpaolo, L.; Wallace, J. L.; Morelli, A. A NO-releasing derivative of paracetamol spares the liver by acting at several checkpoints in the Fas pathway. Br. J. Pharmacol , 2002, 135, 589-599.] It is metabolized in liver by three pathways-glucuronidation, sulfonation (both accounting for 95% of metabolism) or via cytochrome P450 enzyme system (5%) in which it is converted to a toxic metabolite (N- acetyl-p-benzoquinone imine, 2) which further is rendered harmless through an interaction with the endogenous antioxidant glutathione.
Figure imgf000002_0001
(1) (2) But overproduction of this toxic metabolite leads to depletion of glutathione stores in liver and further accumulation of the toxic metabolite causes tissue injury and cell death. [Futter, L. E.; al-Swayeh, O. A.; Moore, P. K. A comparison of the effect of nitroparacetamol and paracetamol on liver injury. Br. J. Pharmacol, 2000, 132, 10-12.] Numerous derivatives of paracetamol have been synthesized to get a safer non-hepatotoxic drug. One of the major achievements has been by Nocox in Italy. It has designed NCX-701 (3) by adding NO moiety to paracetamol, via an aliphatic spacer to improve the anti-inflammatory activity of paracetamol, based on the well-demonstrated activity of NO on cytokine synthesis and regulation of proinflammatory mediators. [Wallace, J. L. Paracetamol hepatotoxicity: NO to the rescue. Br. J. Pharmacol., 2004, 143, 1-2.]
Figure imgf000003_0001
(3)
NO may modulate spinal and sensory neuron excitability through multiple mechanisms that may improve the antinociceptive activity of paracetamol. The pharmacological profile of NCX 701 has been reviewed. [ Marshall, M.; Moore, P. K. Effect of nitric oxide releasing paracetamol and flurbiprofen on cytokine production in human blood. Eur. J. Pharmacol, 2004, 483, 317-322; and al-Swayeh, O. A.; Futter, L. E.; Clifford, R. H.; Moore, P. K. Nitroparacetamol exhibits anti-inflammatory and anti-nociceptive activity. Br. J. Pharmacol, 2000, 130, 1453-1456]. Compared with NSAIDs, paracetamol possesses little anti-inflammatory activity in humans and it does not induce damage of the gastric mucosa, but it could cause liver damage (or even poisoning) when used in high dosage. The aim of coupling paracetamol with NO has, therefore, been to improve its anti-inflammatory activity, and also to exploit the cytoprotective properties of NO to reduce potential liver damage. Concerning safety, several studies have highlighted the liver sparing profile of NCX 701 compared to paracetamol. The beneficial protective effect on the liver has been supposed to be related to the fact that NO can reduce the synthesis of several proinflammatory cytokines. [Wallace, J. L. Paracetamol hepatotoxicity: NO to the rescue. Br. J. Pharmacol, 2004, 143, 1-2]. The anti-inflammatory activity related to NO release has been demonstrated in vitro and in vivo, [Marshall, M.; Moore, P. K. Effect of nitric oxide releasing paracetamol and flurbiprofen on cytokine production in human blood. Eur. J. Pharmacol, 2004, 483, 317-322.; al-Swayeh, O. A.; Futter, L. E.; Clifford, R. H.; Moore, P. K. Nitroparacetamol exhibits anti-inflammatory and anti-nociceptive activity. Br. J. Pharmacol., 2000, 130, 1453-1456.; AND, Paul Clark, M. J.; Howat, D.; Flower, R. J.; Moore, P. K.; Perretti, M. Nitroparacetamol (NCX-701) exhibits anti-inflammatory activity in the zymosan air pouch. Inflamm. Res., 2001, 50, S158] demonstrating that NCX 701 is not only a safer drug than paracetamol, but that it also possesses a wider action due to additive mechanisms. Several papers have compared the antinociceptive activity of NCX 701 versus paracetamol, [Romero-Sandoval, E. A.; Mazario, J.; Howat, D.; Herrero, J. F. NCX-701 (nitroparacetamol) is an effective antinociceptive agent in rat withdrawal reflexes and wind-up. Br. J. Pharmacol, 2002, 135, 1556-1562.; Romero-Sandoval, A. E.; Del Soldato, P.; Herrero, J. F. The effects of sham and full spinalization on the antinociceptive effects of NCX-701 (nitroparacetamol) in monoarthritic rats. Neuropharmacology, 2003, . 45, 412-419.; and Gaitan, G.; Del Soldato, P.; Herrero, J. F. Low doses of nitroparacetamol or dexketoprofen trometamol enhance fentanyl antinociceptive activity. Eur. J. Pharmacol , 2003, 481, 181-188]. regularly showing the superiority of NCX 701. Moreover, sub- effective doses of NCX 701 have enhanced the anti-nociceptive activity of the μ-opioid receptor agonist fentanyl. A Phase II study clinical trial has demonstrated that Ig NCX 701 provides similar analgesic efficacy to paracetamol Ig in post-operative dental pain, therefore with a reduced exposure to paracetamol, confirming a contribution of NO to the anti-nociceptive mechanisms of NCX 701.
O-Dealkylation of a homologous series of alkoxy acetanilides [p-methoxy, p-ethoxy: phenacetin, p-(n)-propoxy- and p-(n)-butoxy acetanilides; 4-7] have shown to release paracetamol.
The compounds (6 and 7) were structurally related to phenacetin (5), and were synthesized to check whether they release paracetamol (1) upon 0-dealkylation, which they did. The chain length in the synthesized compounds was varied to study the effect of chain lengthening on the rate of release of paracetamol.
[Chul-Ho, Y., Miller, G.P., Guengerich, F. P. Oxidations ofp-Alkoxyacylanilides Catalyzed by Human Cytochrome P450 1A2: Structure-Activity Relationships and Simulation of Rate Constants of Individual Steps in Catalysist Biochemistry 2001, 40, 4521-4530] The compounds (4, 6 and 7) are structurally related to phenacetin (5) therefore, on metabolism they will liberate paracetamol (1).
Figure imgf000005_0001
Release of paracetamol (1) by alkoxy acetanilides (4-7)
There was a need to develop the novel molecules which are having better analgesic and anti-inflammatory activity. The advantages of the present invention are that these NO releasing compounds have shown better analgesic and anti-inflammatory activity than the parent drug, and less liver toxicity in biochemical and histopathological studies.
OBJECTS OF THE INVENTION
The main object of the present invention is to provide novel nitrate esters of paracetamol.
Another object of the present invention is to provide process for preparation of novel nitrate esters of paracetamol.
Yet another object of the present invention is to develop nitric oxide releasing derivative of paracetamol that shows better analgesic, anti-inflammatory, biochemical (SGOT, ALP levels) and histopathologic profile. Further object of the present invention is to develop a pharmaceutical composition comprising nitric oxide releasing derivatives of paracetamol.
Still another object of the present invention is to inhibit COX-2 in vitro and release NO in vivo.
Compound, (13) has shown to have better COX-2 (in vitro) inhibition activity i.e. 32.5% than the parent drug paracetamol (1) which had 24.0%. The compounds 12 and 13 also released NO in vivo.
SUMMARY OF THE INVENTION
The present invention relates to the synthesis of NO-releasing derivatives of paracetamol and their evaluation. The compounds showed better analgesic, anti-inflammatory, biochemical and histopathologic profile. The present invention particularly relates to novel nitrate esters of paracetamol. The nitrate esters of paracetamol are prepared by reacting the paracetamol with dihaloalkyl compound and followed by reaction with silver nitrate to obtain the corresponding nitrate ester derivatives.
BRIEF DESCRIPTION OF THE FIGURES
Figure 1: Analgesic activity of paracetamol (1) and NO-NSAIDs (12 and 13)
10 and 11 are intermediates only which were later converted to -ONO2 moiety on treatment with AgNO3
Figure 2: % increase in SGOT levels after 6 hours
Figure 3: % increase in ALP levels after 6 hours
(Explanation for Figure 2 and Figure 3)
Overproduction of toxic metabolite 2 leads to depletion of glutathione stores in liver and further accumulation of the toxic metabolite causes tissue injury and cell death. Enzymes like alkaline phosphatase (ALP, EC 3.1.3.1), serum glutamate pyruvate transaminase
(SGPT) or alanine aminotransferase (ALT) (EC 2.6.1.2) and the total proteins are considered as plasma markers of liver injury. Nitric oxide releasing paracetamol derivatives
(12 and 13) were evaluated for their effect on the markers of liver injury like enzymes ALP and SGPT/ ALT. The plasma levels of these enzymes was found to increase in the case of liver toxicity. The graph indicates that the rise (with comparison to the basal value) in the case of 1 is much more than in the case of NO releasing derivatives (12 and 13), showing that they cause lesser liver toxicity than the parent drug paracetamol (1).
Figure 4: Nitrite ion concentration (μmol/L) in serum after 6 hours
The graph indicates that the serum NO concentration 6 hours after administration of 12 and 13 was greater than the control (0.9% w/v sodium chloride containing 20% v/v tween-80) confirming that these compounds release NO in vivo.
Figure 5: Histopathology (haematoxylin and eosin) of liver samples
Light microscopic analysis of liver samples obtained from mouse treated with control (0.9% w/v sodium chloride containing 20% v/v tween-80), paracetamol (1), (12) and (13), at a dose of 1000 mg/kg, intra peritoneal is shown in sections (A), (B), (C), and (D), respectively. The interpreted results obtained from histopathological studies are summarized below:
(i) Control did not cause any damage to the liver. Only mild portal triad inflammation was observed.
(ii) Paracetamol (1) a proven liver toxic caused ballooning degeneration, sinusoidal decongestion, portal triad inflammation and spoty necrosis in Zone I.
(iii) Just mild ballooning degeneration was observed in the case of 12. (iv) Compound 13 also didn't cause any significant damage to the liver.
These results were supportive in summarizing that NO-release counteracts the liver toxic side effects of paracetamol (1).
DETAILED DESCRIPTION OF THE INVENTION
Accordingly the present invention provides nitrate ester derivatives of paracetamol of general formula A, wherein the value of n is selected from 2 to 5.
Figure imgf000007_0001
Formula A In an embodiment of the invention wherein the structural formula A comprising
Figure imgf000008_0001
(12)
Figure imgf000008_0002
In an embodiment of the invention wherein the representative compounds of the general formula A comprising:
(i) iV-[4-(2-Nitrooxy-ethoxy)-phenyl]-acetamide (12)
(ii) iV-[4-(5-Nitrooxy-pentoxy)-phenyl]-acetamide (13) (iii) iV-[4-(3-Nitrooxy-propoxy)-phenyl]-acetamide (18) (iv) N-[4-(4-Nitrooxy-butoxy)-phenyl]-acetamide (19)
In another embodiment of the invention wherein the compound of formula A is having better peripheral 'analgesic activity and significant anti-inflammatory activity as compared to paracetamol.
In yet another embodiment of the invention wherein the compounds possess better analgesic and antiinfiamatory activity and devoid of toxicity as compared to the parent drug paracetamol (1).
In still another embodiment of the invention wherein the compounds 12 and 13 showed 32.68±1.45% and 31.15±1.31% at 2 hr, and, 26.44±0.10% and 28.45±1.28% at 4 hr increase respectively, in the paw volume as compared to 38.37±1.26% and 58.92±1.56% (at 2 hr and 4hr respectively) in case of paracetamol at a dose of 100mg/kg body weight.
In a further embodiment of the invention wherein the compounds 12 and 13 showed increase in NO serum level 354.2±2.17 and 265.4±2.47 respectively w.r.t. control (182.1±0.68) at a dose of 100mg/kg body weight. In an embodiment of the invention wherein the compound 13 showed in vitro cox-2 (ovine) inhibitory activity 32.5% as compared to control 24.0%.
Accordingly the present invention provides a process for preparation of nitrate ester derivative of paracetamol comprising the steps: a) reacting paracetamol with dibromo alkane in organic solvent in presence of alkali carbonate for a period up to 12 hours, b) filtering the reaction mixture followed by evaporation of solvent to obtain solid residue, c) dissolving the residue in water immisible solvent and washing with sodium hydroxide solution followed by washing with water,evaprating the solvent to O-alkylated bromo derivative of paracetamol, d) reacting the O-alkylated bromo derivative of paracetamol with sliver nitrate under reflux in an aprotic solvent upto 12 hours, further filtering and evaporating the fϊlterate to obtain the nitrate esters derivative of Paracetamol of general formula A wherein the value of n is selected from 2-5.
In an embodiment of the invention wherein dihalo alkane may be selected from a group consisting of 1,2-dibromoethane, 1,3-dibromopropane, 1,4-dibromobutane and 1,5- dibromopentane.
In an embodiment of the invention wherein the organic solvent used may be selected from a group consisting of ethyl methyl ketone, acetone, acetonitrile and mixture thereof.
In an embodiment of the invention wherein the aprotic solvent used may be selected from a group consisting of acetonitrile, benzene, hexane and mixture thereof.
The pharmaceutical composition may be prepared using the compounds of the general formula A along with the pharmaceutically acceptable excipients.
Compounds 12 and 13 are novel and have not been reported earlier. Compound (13) has shown to have better COX-2 inhibition activity (in vitro) than the parent drug paracetamol. The data of analgesic and anti-inflammatory activities is exactly complimenting and supporting it. Therefore, it can be concluded that the NO releasing compound is showing better activity than the parent drug candidate, and moreover, the biochemical and histopathological studies carried out, indicate that the compound is causing less liver toxicity than paracetamol.
NO-releasing derivatives of paracetamol, (12) and (13) showed good anti-inflammatory activity w.r.t. parent drug, both at 2 hr and 4 hr interval. No significant activity was observed in case of paracetamol (1). O-alkylated NO-releasing derivatives (12 and 13) of paracetamol (1) exhibited better response than the parent drug indicated by greater % inhibition in writings. The intermediates 10 and 11, having terminal Br (which was later converted to -ONO2 moiety on treatment with AgNO3) showed very negligible % inhibition in writings indicating that NO release is responsible for better analgesic activity of the compounds 12 and 13 (having NO releasing -ONO2) moiety. In NO releasing activity they also showed significant increase in level of NO. The NO release of these compounds could be the acting force behind their better analgesic and anti-inflammatory activities than the parent drug. Our compound (13) has shown to have better COX-2 (in vitro) than the parent drug paracetamol. In the case of biochemical studies paracetamol (1) caused a significant increase in the serum ALP levels as compared to the NO-releasing derivatives (12 and 13), indicating its liver toxicity. Both the derivatives were found to increase the levels of ALP but not more than the drug. Increase in the levels of SGPT (ALT) 6 hr after administration of paracetamol (1) justified its liver toxicity, with % increase more than 80%. Increase in the case of both the derivatives (12 and 13) was not that significant. In histopathological studies, also the damage caused by 12 and 13, to liver was less in comparison to paracetamol (1). Therefore, compounds 12 and 13 showed better activity and lesser toxicity than the parent drug 1.
Paracetamol (1), a proven liver toxic and having no anti-inflammatory activity of its own has been converted to more active and lesser toxic NO releasing compounds 12 and 13.
Nitric oxide releasing derivatives of paracetamol are developed (12 and 13) (Figure 2), which have shown better analgesic, anti-inflammatory, biochemical (SGOT, ALP levels) and histopathologic profile. Besides these derivatives have shown to inhibit COX-2 in vitro and release NO in vivo. The liver toxic drug paracetamol (1) having no anti-inflammatory activity has been converted to less liver toxic and more active derivatives. Briefly, Paracetamol was stirred in a solution of 1, 2-dibromoethane (8) and 1, 5-dibromopentane (9), in ethyl methyl ketone and K2CO3 for 8-10 hours. The resulting solution was filtered, dried and evaporated to get a solid residue. The residue was dissolved in CHCl3 and washed with 5% NaOH (3 X 100ml) and water (3 X 100ml), dried over anhydrous Na2SO4, filtered and solution evaporated to get O-alkyl derivatives 10 and 11, respectively. These were refluxed in a solution OfAgNO3 and acetonitrile overnight, filtered, and evaporated to get nitrate esters 12 and 13, respectively.
Figure imgf000011_0001
Synthesis of NO releasing derivatives (12 and 13) of paracetamol (1)
Following examples are given by way of illustration and should not construed to limit the scope of the invention
Example 1
N- [4-(2-Bromo-ethoxy)-phenyl] -acetamide ( 10)
A solution of jV-(4-hydroxyphenyl)-acetamide (paracetamol) (1) (1.50 g, 0.01 mol) and 1,2- dibromoethane (8) (10.0 ml) in ethyl methyl ketone (25.0 ml) containing potassium carbonate (5.0 g) was stirred for 8 hr at room temperature. The reaction mixture was filtered, residue washed with ethyl methyl ketone and solvent evaporated under reduced pressure to get the residue. The residue was dissolved in chloroform (200.0 ml), washed with 5% NaOH solution (3 x 100 ml), water (3 x 50 ml), dried, filtered and crystallized from methanol to get the desired product iV-[4-(2-bromo-ethoxy)-phenyl]-acetamide (10) (2.01 g, 78.51 %), mp 126-1270 C.
Analysis:
IR (KBr): 3302, 3024, 1662, 1551, 1242, 1029 and 828 crn 1
1H NMR (CDCl3): δ 2.10 (s, 3H, -NHCOCH3), 3.65 (t, 2Η, J = 5.9 Hz, CH2Br), 4.26 (t, 2Η, J = 5.9 Hz, -OCH2), 6.83 (d, 2Η, Ar-H), 7.49 (d, 2Η, Ar-H), 9.42 (s, 1Η, -NH Exchangeable with D2O)
13C NMR (CDCl3): δ 23.49 (-NHCOCH3), 29.03 (-CH2Br-), 67.66 (-OCH2-), 114.40 (2 * 2° Ar-Q, 121.06 (2 x 2° Ar-C), 132.20 (1 x 3° Ar-Q, 153.72 (1 x 3° Ar-Q and 168.26 (- NHCOCH3)
Calcd. for C10H12BrNO2 : C, 46.53; H, 4.69; N, 5.43. Found: C, 46.77; H, 4.17; N, 5.24.
iV-[4-(2-Nitrooxy-ethoxy)-phenyI]-acetamide (12)
A mixture of jV-[4-(2-bromo-ethQxy)-phenyl]-acetamide (10) (1.30 g, 0.005 mol), silver nitrate (5.0 g) and freshly distilled acetonitrile (50.0 ml) was refluxed for 10 hr, cooled, poured into crushed ice and extracted with chloroform (3 x 100 ml). The combined organic layers were dried, filtered, solvent removed under reduced pressure to obtain a residue which was crystallized from methanol to obtain iV-[4-(2-nitrooxy-ethoxy)-phenyl]- acetamide (12) (0.91 g, 75.83 %), mp 106-107° C.
Anal.
IR (KBr): 3324, 3060, 2920, 1667, 1634, 1522, 1238, 1046 and 861 cm"1
1H NMR (CDCl3): δ 2.15 (s, 3H, -NHCOCH3), 4.21 (t, 2Η, J = 4.6 Hz, -OCH2-), 4.80 (t, 2Η, J = 4.6 Hz, -CH2ONO2), 6.83 (d, 2Η, Ar-H), 7.49 (d, 2Η, Ar-H) and 7.43 (s, 1Η, -NH Exchangeable with D2O) 13C NMR (CDCl3): δ 24.32 (-NHCOCH3), 64.46 (-CH2ONO2-), 71.09 (-OCH2-), 115.00 (2 x 2° Ar-Q, 122.02 (2 x 2° Ar-C), 132.03 (1 * 3° Ar-Q, 154.74 (1 x 3° Ar-C) and 168.62 (- NHCOCH3)
Calcd. for C10H12N2O5 : C, 50.00; H-, 5.04; N, 1 1.66. Found: C, 49.76; H, 4.60; N, 1 1.19.
Example 2 iV-[4-(5-Bromo-pentoxy)-phenyI]-acetamide (ll)
A solution of Λr-(4-hydroxyphenyl)-acetamide (paracetamol) (1) (1.50 g, 0.01 mol) and 1,5- dibromopentane (9) (10.0 ml) in ethyl methyl ketone (25.0 ml) containing potassium carbonate (5.0 g) was stirred for 8 hr at room temperature. The reaction mixture was filtered, residue washed with ethyl methyl ketone and solvent evaporated under reduced pressure to get the residue. The residue was dissolved in chloroform (200.0 ml), washed with 5% sodium hydroxide solution (3 x 100 ml), water (3 * 50 ml), dried, filtered and crystallized from methanol to get the desired product 7V-[4-(5-bromo-pentoxy)-phenyl]- acetamide (11) (2.15 g, 72.15 %), mp 110-111° C.
Anal.
IR (KBr): 3303, 3031, 2944, 1660, 1549, 1410, 1239, 1045 and 735 cπf1
1H NMR (CDCl3): δ 1.62 (m, 2H, -CH2CH2CH2-), 1.81 (p, 2H, J = 7.4 Hz, - OCH2CH2-), 1.92 (m, 2Η, -CH2CH2Br-), 2.11 (s, 3H, -NHCOCH3), 3.43 (t, 2Η, J = 6.3 Hz, -CH2Br), 3.92 (t, 2Η, J = 6.3Hz, -OCH2-), 6.81 (d, 2Η, Ar-H), 7.37 (d, 2Η, Ar-H) and 9.42 (s, 1Η, - NH Exchangeable with D2O)
13C NMR (CDCl3): δ 24.20 (-NHCOCH3), 24.83 (-CH2CH2CH2-), 28.45 (-OCH2CH2-), 32.49 (-CH2CH2Br-), 33.68 (-CH2Br-), 67.87 (-OCH2-), 114.72 (2 x 2° Ar-Q, 122.06 (2 x 2° Ar-Q, 131.05 (1 x 3° Ar-Q, 155.84 (1 x 3° Ar-Q and 168.70 (-NHCOCH3)
Calcd. for CnH18BrNO2 : C, 52.01 ; H, 6.04; N, 4.67. Found: C, 52.67; H, 5.54; N, 4.74. iV-[4-(5-Nitrooxy-pentoxy)-phenyl]-acetainide (13)
A mixture of N-[4-(5-bromo-pentoxy)-phenyl]-acetamide (11) (1.50 g, 0.005 mol)), silver nitrate (5.0 g) and freshly distilled acetonitrile (50.0 ml) was refluxed for 10 hr, cooled, poured into crushed ice and extracted with chloroform (3 x 100 ml). The combined organic layers were dried, filtered, solvent removed under reduced pressure to obtain a residue which was crystallized from methanol to obtain N-[4-(5-nitro oxy-pentoxy)-phenyl]- acetamide (13) (1.02 g, 72.34 %), mp 96-98° C.
Anal.
IR (KBr): 3322, 3025, 2951, 1663, 1547, 1236, 1048, 878 and 739 cm"1
1H NMR (CDCl3): δ 1.59 (m, 2H, -CH2CH2CH2-), 1.84 (m, 4H, -CH2CH2CH2-), 2.10 (s, 3H, -NHCOCH3), 3.93 (t, 2H, J = 6.6 Hz, -OCH2-), 4.49 (t, 2H, J = 6.6 Hz, -CH2ONO2), 6.80 (d, 2H, Ar-H), 7.45 (d, 2H, Ar-H), 9.10 (s, IH, -NH, Exchangeable with D2O)
13C NMR (CDCl3): δ 22.52 (-NHCOCH3), 24.36 (-CH2CH2CH2-), 26.64 (-OCH2CH2-), 28.83 (-CH2CH2ONO2-), 67.74 (-CH2ONO2-), 73.23 (-OCH2-), 114.80 (2 x 2° Ar-C), 122.01 (2 x 2° Ar-C), 131.11 (1 x 3° Ar-C), 155.83 (1 x 3° Ar-C) and 168.40 (-NHCOCH3)
Calcd. for C13H18N2O5 : C, 55.31; H, 6.43; N, 9.92. Found: C, 54.89; H, 6.19; N, 9.47.
Compounds 10 and 11 are the intermediates and are synthesized as per literature references:
(10): A Multi-Mode-Driven Molecular Shuttle: Photochemically and Thermally Reactive Azobenzene Rotaxanes. Murakami, Hiroto; Kawabuchi, Atsushi; Matsumoto, Rika; Ido, Takeshi; Nakashima, Naotoshi. Department of Applied Chemistry, Faculty of
Engineering, Nagasaki University, Nagasaki, Japan. Journal of the American Chemical Society (2005), 127(45), 15891-15899.
(11): Antagonists of slow reacting substance of anaphylaxis. Synthesis of a series of chromone-2-carboxylic acids. Appleton, R. A.; Bantick, J. R.; Chamberlain, T. R.;
Hardern, D. N.; Lee, T. B.; Pratt, A. D. Pharm. Div., Fisons Ltd., Loughborough/Leicestershire, UK. Journal of Medicinal Chemistry (1977), 20(3), 371-9.
Examples of compounds with C3 and C4 chains are given below;
Figure imgf000015_0001
Example 3: iV-f4-(3-Bromo-propoxγ)-phenvIl-acetamide (16)
A solution of Λr-(4-hydroxyphenyl)acetamide (paracetamol) (1) (1.50 g) and 1,3- dibromobutane (14) (10.0 ml) in ethyl methyl ketone (25.0 ml) containing potassium carbonate (5.0 g) was stirred overnight at room temperature. The reaction mixture was filtered, residue washed with ethyl methyl ketone and solvent evaporated under reduced pressure to get the residue. The residue was dissolved in chloroform (200.0 ml), washed with 5% NaOH solution (3 x 100 ml), water (3 * 50 ml), dried, filtered and crystallized from methanol to get the desired product JV-[4-(3-Bromo-propoxy)-phenyl]-acetamide (16) ( 2.01g , 75.8%), mp 115-118 'C
Analysis:
IR (KBr): 3284, 1658, 1559, 1410, 1239, 1032, 832 and 521 cm"1
1H NMR (400 MHz, CDCl3): δ 2.14 (s, 3H, -NHCOCH3), δ 2.30 (p, 2H-J=O-I, - CH2CH2Br-), δ 3.59 (t, 2H, J=6.4 Hz, -CH2Br), δ 4.07 (t, 2H, J=5.8 Hz, -OCH2-), δ 6.86 (d, 2H, J=3.0 Hz, Ar-H), δ 7.37 (d, 2H, J=3.0 Hz, Ar-H) and δ 7.27(s, IH, -NH, Exchangeable with D2O) 13C NMR (400 MHz, CDCl3): δ 24.40 (-NHCOCH3), δ 30.14 (-CH2CH2CH2Br-), δ 32.36 (-CH2Br-), δ 65.58 (-OCH2-), δ 114.84 (2 x 2° Ar-C), δ 122 (2 x 2° Ar-C), δ 131.23 (1 x 3° Ar-C), δ 155.55 (1 x 3° Ar-C) and δ 168.43 (-NHCOCH3)
CHN: Calculated: C, 48.55; H, 5.19; N, 5.15. Found: C; 48.76; H, 5.10; N, 4.36. Λr-f4-(3-Nitrooxy-propoxy)-phenyll-acetamide (18)
A mixture of JV-[4-(3-Bromo-propoxy)-phenyl]-acetamide (16) (1.50 g), silver nitrare (5.0 g) and freshly distilled acetonitrile (50.0 ml) was refluxed for 10 hr, cooled, poured into crushed ice and extracted with chloroform (3 x 100 ml). The combined organic layers were dried, filtered, solvent removed under reduced pressure to obtain a residue which was crystallized from methanol to obtain iV-[4-(3-Nitro oxy-propoxy)-phenyl]-acetamide (18) (1.09g ,77.30%) , mp 89-92'C.
Analysis
IR (KBr: 3333, 1664, 1548, 1411, 1238, 1057, 839 and 704 cm"1
1H NMR (400 MHz, CDCl3): δ 2.14 (s, 3H, -NHCOCH3), δ 2.19 (p, 2H,J =6.1 - CH2CH2CH2-), δ 4.66 (t, 2H, J =6.3 Hz, -OCH2-), δ 4.03 (t, 2H, J=5.9Hz, -CH2ONO2), δ 6.84 (d, 2H, ./=3.1 Hz, Ar-H), δ 7.41 (d, 2H, J=3.8 Hz, Ar-H) and δ 7.26(s, IH, -NH, • Exchangeable with D2O)
13C NMR (400 MHz, CDCl3): δ 24.40 (-NHCOCH3), δ 27.0 (-CH2CH2CH2-), δ 63.80 (- CH2ONO2-), δ 70.00 (-OCH2), δ 77.11 (-OCH2-), δ 114.76 (2 x 2° Ar-C), δ 121.98 (2 x 2° Ar-C), δ 131.46 (1 x 3° Ar-C), δ 155.27 (1 x 3° Ar-C) and δ 168.46 (-NHCOCH3)
CHN: Calculated: C, 51.97; H, 5.55; N, 11.02. Found: C, 48.63; H, 5.37; N, 10.79.
Example 4 yV-[4-(4-Bromo-butoxy)-phenyl|-acetamide (17)
A solution of 7V-(4-hydroxyphenyl) acetamide (Paracetamol) (1) (1.50 g) and 1,3- dibromobutane (15) (10.0 ml) in acetonitrile (25.0 ml) containing potassium carbonate (5.Og) was refluxed for 8 hrs. at room temperature. The reaction mixture was filtered and the filtrate was poured into ice cold water. The residue was filtered and washed with 5% NaOH solution (3 * 100 ml), water (3 x 50 ml), dried, filtered and crystallized from methanol to get the desired product N-[4-(4-bromobutoxy)phenyl] acetamide (17) (2.15g ,77.06 ) , mp 119-124 ° C.
Analysis:
IR (KBr): 3289, 1660, 1552, 1409, 1236, 1046, 825 and 520 cm-1
1H NMR (400 MHz, CDCl3): δ 2.04 (s, 3H, -NHCOCH3), δ 2.06 (p, 2H, J=2.13 Hz, (- CH2CH2CH2Br), δ 1.94 (p, 2H, J=3.35 Hz, (-CH2CH2CH2CH2 Br), δ 3.48 (t, 2H, J=6.6 Hz, CH2Br), δ 3.98 (t, 2H, J=6.0 Hz, -OCH2), δ 6.85 (d, 2H, J=3.0 Hz, Ar-H), δ 7.37 (d, 2H, J=3.0 Hz, Ar-H), δ 7.26 (s, IH, -NH Exchangeable with D2O)
13C NMR (400 MHz, CDCl3): δ 24.38 (-NHCOCH3), δ 27.91(-CH2CH2CH2CH2Br), δ 29.48 (-CH2CH2CH2 Br), δ 33.60 (-CH2Br-), δ 67.10 (-OCH2-), δ 114.72 (2 x 2° Ar-C), δ 121.99 (2 x 2° Ar-C), δ 131.07 (1 * 3° Ar-C), δ 155.72 (1 x 3° Ar-C) and δ 168.46 (- NHCOCH3)
CHN: Calculated: C, 50.37; H, 5.64; N, 4.89. Found: C, 49.96; H, 5.73; N, 4.68. iV-f4-(4-Nitro oxy-butoxy)-phenv-l-acetamide (19)
A mixture of iV-[4-(4-bromobutoxy)phenyl] acetamide (17) (1.30 g), silver nitrare (5.0 g) and. freshly distilled acetonitrile (50.0 ml) was refluxed for 10 hr, cooled, poured into crushed ice and extracted with chloroform (3 x 100 ml). The combined organic layers were dried, filtered, solvent removed under reduced pressure to obtain a residue which was crystallized from methanol to obtain N-[4-(4-Nitro oxy-butoxy)-phenyl]- acetamide (19) (l.lOg ,78%) , mp 92-98° C.
Analysis:
IR (KBr): 3327, 1658, 1519, 1408, 1238, 1040, 832 and 551 cm'1
1H NMR (400 MHz, CDCl3):, δ 2.07 (s, 3H, -NHCOCH3), δl.88 (p, 2H, J=2.7 Hz, -CH2CH2CH2CH2ONO2), δl.79 (p, 2H, J=2.9 Hz5-CH2CH2CH2 ONO2), δ 3.91 (t, 2H, J=5.68 Hz, -CH2ONO2), δ4.46 (t, 2H, J= 6.2Hz, -OCH2-), δ 6.76 (d, 2H, J=3.09 Hz, Ar-H), δ 7.32 (d, 2H, J= 3.09Hz, Ar-H) and δ 7.30 (s, IH, -NH, Exchangeable with D2O) 13C NMR (30 MHz, CDCl3): δ 23.89 (-NHCOCH3), δ 25.60 (-CH2CH2CH2CH2ONO2), δ 24.43 (-CH2CH2CH2ONO2), δ 67.22 (-CH2ONO2-), δ 73.02 (-OCH2-), δ 114.75 (2 x 2° Ar-C), δ 121.99 (2 x 2° Ar-C), δ 131.16(1 * 3° Ar-C), δ 155.61 (1 * 3° Ar-C) and δ 168.36 (-NHCOCH3)
CHN: Calculated: C, 53.73; H, 6.01; N, 10.44. Found: C, 52.94; H, 6.01; N, 9.90.
Biological Activity :
Animals: Wistar rats (male 150-20Og) of both sex and Laca mice (male, 25-35g) procured from Central Animal House, Punjab University, Chandigarh, India were used. Animals were housed under standard laboratory conditions and maintained on rat chow. Animals were allowed free access to food and water until used and fasted 24 hr prior to studies.
Experimental conditions: Unless otherwise stated, the following conditions were employed in all experiments. The test compounds were suspended in 0.5% carboxymethylcellulose (CMC) and administered per orally (p.o.). Control animals were given the corresponding amount of vehicle (0.5%, CMC).
Anti-inflammatory activity (Table 1): Anti-inflammatory activity was determined by using carrageenan induced rat paw edema model. Rats were divided into different groups and the drugs were administered to each group. The paracetamol (1) and synthesized NO- releasing derivatives (12 and 13) were administered p.o. at a dose of 100 mg/kg, p.o., emulsified in 0.5% sodium carboxy methyl cellulose (0.5% sodium CMC). Anti- inflammatory activity was determined by using carrageenan induced rat paw edema model. Rats were divided into different groups and the drugs/derivatives were administered to each group. Acute edema was induced in left hind paw of rats by injecting freshly prepared solution of carrageenan (Type IV, 0.1ml, 1%) under plantar region of left hind paw. In the right paw, saline (ImI, 0.9%) was injected, which served as control for comparison. The increase in paw volume was measured by using plethysmometer (water displacement, UGO BASILE, Varese, Italy) at 2 hr and 4 hr after carrageenan challenge. Percentage change (increase) in paw volume was calculated and expressed as the amount of inflammation.
% increase in paw volume at any time = (Vi-Vr)/Vr x 100 Where, Vi = Volume of left paw
Vr = Volume of right paw (control)
Figure imgf000019_0001
Table 1: Anti-inflammatory activity of paracetamol (1) and NO-NSAIDs (12 and 13)
Results are expressed as mean ± standard error of mean (SEM).
* indicates P<0.05 w.r.t. control (0.5% sodium CMC); and
# indicates P<0.05 w.r.t. paracetamol (100 mg/kg p.o.)
NO-releasing derivatives of paracetamol (1), 12 and 13 showed good anti-inflammatory activity w.r.t. parent drug 1, both at 2 hr and 4 hr interval (shown by less % increase in paw volume). At 2 hr % increase in paw volume for 12 and 13 was 32.68±1.45 and 31.15±1.31 respectively, and at 4 hr % increase in paw volume for 12 and 13 was 26.44±0.10 and 28.45±1.28 respectively. No significant activity was observed in case of paracetamol (1) as % increase in paw volume at 2 hr and 4 hr was found to be 38.37±1.26 and 58.92±1.56 respectively.
Analgesic activity (Table 2 and FIG. 1 Sheet No. 1 Figure 3): Analgesic activity was determined by using abdominal writhing assay. Mice were divided into different groups containing 6 animals in each group. Analgesic activity was determined against acetic acid induced writhing assay. Writhing was induced by intraperitoneal (i.p.) injection of freshly prepared acetic acid solution. Mice were per orally (p.o.) administered paracetamol (1) and synthesized NO-releasing derivatives (12 and 13) at a dose of 100 mg/kg, emulsified in 0.5% sodium carboxy methyl cellulose (sodium CMC) vehicle, 30 min prior to i.p. acetic acid (1% w/v in saline pH 2.7, 10 ml/kg, i.p.). Animals were immediately transferred to individual observation chambers and number of writhes (constriction of abdomen, turning of trunk, and extension of hind limbs) were monitored over the following 20 minutes, beginning 3 min after the injection of acetic acid. At the end of observation period the animals were killed by cervical dislocation and exsaguination. The average number of writhes in each group of drug treated mice was compared with that of the control group and degree of analgesia was expressed as % inhibition calculated from the equation :
% Inhibition = (1-Nt/Nc) x 100 Where, Nc = number of writhes in control
Nt = number of writhes in drug treated mice
Figure imgf000020_0001
Table 1: Analgesic of paracetamol (1) and NO-NSAIDs (12 and 13) Results are expressed as mean ± standard error of mean (SEM). # indicates P<0.05 w.r.t. Paracetamol (100 mg/kg p.o.)
Oalkylated NO-releasing derivatives 12 and 13 of paracetamol (1) exhibited better response than the parent drug indicated by greater % inhibition in writings. The % inhibition in case of 12 and 13 (58.35±1.50, 61.80±1.69 respectively) was found to be better than paracetamol (1) which was 45.36±1.34. The intermediates 10 and 11, having terminal Br (which was later converted to -ONO2 moiety on treatment with AgNO3) showed very negligible % inhibition in writings indicating that NO release is responsible for better analgesic activity of the compounds 12 and 13 (having NO releasing -ONO2) moiety.
Biochemical Assays (Table 3 and FIG. 2 Sheet No. 1 for SGPT(ALT), and Table 4 and FIG. 3 Sheet No.2 for ALP): Paracetamol was given at a dose of 1000 mg/Kg and an equivalent dose of 1000 mg/Kg of the derivatives 12 and 13 was given p.o. Before administration blood samples of each animal were taken and after 6 hrs the animals were sacrificed to collect blood samples, and the serum was separated out. Measurement of plasma markers of liver Serum Glutamate oxaloacetate transaminase (SGOT) pyruvate transaminase (SGPT) and Alkaline Phosphatase (ALP) were assayed spectrophotometrically using commercially available kits. The animals were divided into different groups containing 6-8 animals in each group. Animals were lightly anaesthetized with diethyl ether and blood samples were collected which served as the basal reading of the enzyme to be analyzed. These groups of rats were then treated with paracetamol (1000 mg/Kg, Lp.), aηd equivalent dose of the test compounds. Control group was treated with an equal volume of vehicle (0.9% w/v sodium chloride containing 20% v/v tween-80). Animals were lightly anesthetized with ethyl ether and blood samples were collected and allowed to coagulate at room temperature for 1 hr, serum was then separated by centrifugation (5,000 rpm for 10 min) at 4°C and stored at -
40.0 C till estimation. Plasma markers of liver injury were assayed spectrophotometrically using following commercially available kits according to standard laboratory techniques:
Figure imgf000021_0001
Table 3: % increase in SGPT/ALT level
Results are expressed as mean ± standard error of mean (SEM).
* indicates PO.05 w.r.t. control (0.5% sodium CMC); and,
# indicates P<0.05 w.r.t. paracetamol (1000 mg/kg, i.p.)
Figure imgf000021_0002
Table 4: % increase in ALP level
Results are expressed as mean ± standard error of mean (SEM).
* indicates P<0.05 w.r.t. control (0.5% sodium CMC); and,
# indicates P<0.05 w.r.t. paracetamol (1000 mg/kg, i.p.)
Overproduction of this toxic metabolite 2 leads to depletion of glutathione stores in liver and further accumulation of the toxic metabolite causes tissue injury and cell death. Enzymes like alkaline phosphatase (ALP, EC 3.1.3.1), serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALT) (EC 2.6.1.2) and the total proteins are considered as plasma markers of liver injury. Nitric oxide releasing paracetamol derivatives 12 and 13 were evaluated for their effect on the markers of liver injury like enzymes ALP and SGPT/ ALT. The plasma level of these enzymes is found to increase in the case of liver toxicity. The graph indicates that the rise (with comparison to the basal value) in the case of 1 is much more than in the case of NO releasing derivatives 12 and 13, showing that they cause lesser liver toxicity than the parent drug paracetamol 1.
Histopathology (FIG. 5 Sheet No. 3) For liver histopathology, the livers were removed and portions were fixed in 10% buffered formalin (pH 7.4). They were then processed by standard histopathological techniques, stained with Haematoxylin and Eosin (H&E), and examineed for morphologic evaluation for liver injury.21
Light microscopic analysis of liver samples obtained from mouse treated with control (0.9% w/v sodium chloride containing 20% v/v tween-80), paracetamol (1), 12 and 13, at a dose of 1000 mg/kg, i.p. is shown in sections (A), (B), (C), and (D) respectively. The interpreted results obtained from histopathological studies are summarized below:
(i) Control did not cause any damage to the liver. Only mild portal triad inflammation was observed.
(ii) Paracetamol (1) a proven liver toxic caused ballooning degeneration, sinusoidal decongestion, portal triad inflammation and spoty necrosis in Zone I.
(iii) Just mild ballooning degeneration was observed in the case of 12. (iv) Compound 13 also didn't cause any significant damage to the liver.
These results were supportive in summarizing that NO-release counteracts the liver toxic side effects of paracetamol (1).
Nitric Oxide Release {Table 5 and FIG 4 Sheet No. 2, Fig 5} :
100 mg/Kg of the derivatives 12 and 13 was given p.o. After 6 hours the animals were sacrificed to collect blood samples and the serum was separated out to find out the levels of NO. Plasma nitrate/nitrite concentration level was measured by commercially available Cayman Kit.
Figure imgf000023_0001
Table 4: % increase in ALP level
Results are expressed as mean ± standard error of mean (SEM).
* indicates P<0.05 w.r.t. control (0.5% sodium CMC)
After a period of 6 hr 12 and 13, NO-releasing derivatives showed increase in % NO serum level w.r.t. control. The NO release of these compounds could be the acting force behind their better analgesic and anti-inflammatory activities than the parent drug.
In vitro cox-2 (ovine) inhibitory assay: The prostaglandin product is quantified via enzyme immunoassay (EIA) using a broadly specific antibody that binds to all major prostaglandin compounds. Assay kit was purchased from Cayman Chemical Company, USA.
Celecoxib: 79.0% (positive control)
PCM: 24.0%
12: 21.0%
13: 32.5%
Our compound" 13 has shown to have better COX-2 (in vitro) than the parent drug paracetamol. The data of analgesic and anti-inflammatory activities is exactly complimenting and supporting it.
The NO-releasing should have been found to have better peripheral analgesic activity and significant anti-inflammatory activity as compared to the parent drug. The derivatives were found to release NO in vivo and are devoid of liver toxicity as indicated by serum lesser increase in serum GPT(ALT) and ALP levels and histopathological studies. Moreover, 13 showed better COX-2 (ovine) binding activity than the parent drug. On the whole more effective and less toxic derivatives of 1 have been synthesised.
The advantages of the present investigation are that these NO releasing compounds have shown better analgesic and anti inflammatory activity than the parent drug, and less liver toxicity in biochemical and histopathological studies.

Claims

We claim:
1. Nitrate ester derivatives of paracetamol of general formula A, wherein the value of n is selected form 2 to 5.
Figure imgf000024_0001
Formula A
2. A compound according to claim 1 wherein the structural formula comprising
Figure imgf000024_0002
(12)
Figure imgf000024_0003
3. A compound according to claim 1 wherein the representative compounds of the general formula comprising:
(i) N-[4-(2-Nitrooxy-ethoxy)-phenyl]-acetamide (12) (ii) iV-[4-(5-Nitrooxy-pentoxy)-phenyl]-acetamide (13) (iii) N-[4-(3-Nitrooxy-propoxy)-phenyl]-acetamide (18) (iv) Λr-[4-(4-Nitrooxy-butoxy)-phenyl]-acetamide (19).
4. A compound according to claim 1 wherein the compound of formula A is having better peripheral analgesic activity and significant anti-inflammatory activity as compared to paracetamol.
5. A compound according to claim 1 wherein the compounds possess better analgesic and anti inflamatory activity and devoid of toxicity as compared to the parent drug paracetamol (1).
6. A compound according to claim 1 wherein the compounds 12 and 13 "showed 32.68±1.45% and 31.15± 1.31% at 2 hr, and, 26.44±0.10% and 28.45±1.28% at 4 hr increase respectively, in the paw volume as compared to 38.37±1.26% and 58.92±1.56% (at 2 hr and 4hr respectively) in case of paracetamol at a dose of lOOmg/kg body weight.
7. A compound according to claim 1 wherein the compounds 12 and 13 showed increase in NO serum level 354.2±2.17 and 265.4±2.47 respectively w.r.t. control (l82.1±0.68) at a dose of 100mg/kg body weight.
8. A compound according to claim 1 wherein the compound 13 showed in vitro cox-2 (ovine) inhibitory activity 32.5% as compared to control 24.0%.
9. A process for preparation of nitrate ester derivative of paracetamol comprising the steps a) reacting paracetamol with dibromo alkane in an organic solvent in presence of alkali carbonate for a period up to 12 hours, b)filtering the reaction mixture followed by evaporation of solvent to obtain solid residue, c)dissolving the residue in water immisible solvent and washing with sodium hydroxide . solution followed by washing with water,evaporating the solvent to O-alkylated bromo derivative of paracetamol, d)reacting the O-alkylated bromo derivative of paracetamol with sliver nitrate under reflux in an aprotic solvent upto 12 hours,further filtering and evaporating the filterate to obtain the nitrate esters derivative of paracetamol of general formula A wherein the value of n is selected from 2-5.
10. A process according to claim 8, wherein dihalo alkane is selected from a group consisting of 1,2-dibromoethane, 1,3-dibromopropane, 1 ,4-dibromobutane and 1,5- dibromopentane.
11. A process according to claim 8, wherein the organic solvent used is selected from a group consisting of ethyl methyl ketone, acetone, acetonitrile and mixture thereof.
12. A process according to claim 8, wherein the aprotic solvent used is selected from a group consisting of acetonitrile, benzene, hexane and mixture thereof.
13. A pharmaceutical composition comprising the compounds of general formula A of claim 1 , and pharmaceutically acceptable excipients.
PCT/IN2008/000514 2007-08-17 2008-08-14 Nitric oxide releasing derivatives of paracetamol WO2009024998A1 (en)

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WO2003084550A1 (en) 2002-04-11 2003-10-16 Nicox S.A. Drugs for the arthritis treatment
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WO2001012584A2 (en) 1999-08-12 2001-02-22 Nicox S.A. Pharmaceutical compounds
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