WO2008153935A3 - Methods for improving protein properties - Google Patents

Methods for improving protein properties Download PDF

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Publication number
WO2008153935A3
WO2008153935A3 PCT/US2008/007114 US2008007114W WO2008153935A3 WO 2008153935 A3 WO2008153935 A3 WO 2008153935A3 US 2008007114 W US2008007114 W US 2008007114W WO 2008153935 A3 WO2008153935 A3 WO 2008153935A3
Authority
WO
WIPO (PCT)
Prior art keywords
provides methods
present
optimize
environmental conditions
enzymes
Prior art date
Application number
PCT/US2008/007114
Other languages
French (fr)
Other versions
WO2008153935A2 (en
Inventor
Wolfgang Aehle
Luis Gustavo Cascao-Pereira
Jr James T Kellis
Andrew Shaw
Original Assignee
Danisco Us Inc Genencor Div
Wolfgang Aehle
Luis Gustavo Cascao-Pereira
Jr James T Kellis
Andrew Shaw
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc Genencor Div, Wolfgang Aehle, Luis Gustavo Cascao-Pereira, Jr James T Kellis, Andrew Shaw filed Critical Danisco Us Inc Genencor Div
Priority to DK08768190.4T priority Critical patent/DK2171057T3/en
Priority to EP08768190.4A priority patent/EP2171057B1/en
Priority to CN200880101988.3A priority patent/CN101784662B/en
Priority to BRPI0812448 priority patent/BRPI0812448A2/en
Priority to MX2009013084A priority patent/MX2009013084A/en
Priority to US12/602,989 priority patent/US20100233780A1/en
Priority to JP2010511201A priority patent/JP5674462B2/en
Priority to CA2690032A priority patent/CA2690032C/en
Priority to RU2009149662/10A priority patent/RU2520808C2/en
Publication of WO2008153935A2 publication Critical patent/WO2008153935A2/en
Publication of WO2008153935A3 publication Critical patent/WO2008153935A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides methods for engineering proteins to optimize their performance under certain environmental conditions of interest. In some embodiments, the present invention provides methods for engineering enzymes to optimize their catalytic activity under particular environmental conditions. In some preferred embodiments, the present invention provides methods for engineering enzymes to optimize their catalytic activity and/or stability under adverse environmental conditions. In some preferred embodiments, the present invention provides methods for engineering enzymes to optimize their storage stability, particularly under adverse environmental conditions. In some preferred embodiments, the present invention provides methods for altering the net surface charge and/or surface charge distribution of enzymes (e.g., metalloproteases) to obtain enzyme variants that demonstrate improved performance and/or stability in detergent formulations as compared to the starting or parent enzyme.
PCT/US2008/007114 2007-06-06 2008-06-06 Methods for improving protein properties WO2008153935A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DK08768190.4T DK2171057T3 (en) 2007-06-06 2008-06-06 Methods to improve the protein properties
EP08768190.4A EP2171057B1 (en) 2007-06-06 2008-06-06 Methods for improving protein properties
CN200880101988.3A CN101784662B (en) 2007-06-06 2008-06-06 Methods for improving protein properties
BRPI0812448 BRPI0812448A2 (en) 2007-06-06 2008-06-06 PROTEIN PROPERTY PROCESSING METHOD
MX2009013084A MX2009013084A (en) 2007-06-06 2008-06-06 Methods for improving protein properties.
US12/602,989 US20100233780A1 (en) 2007-06-06 2008-06-06 Methods for Improving Protein Properties
JP2010511201A JP5674462B2 (en) 2007-06-06 2008-06-06 Methods for improving protein properties
CA2690032A CA2690032C (en) 2007-06-06 2008-06-06 Methods for improving protein properties
RU2009149662/10A RU2520808C2 (en) 2007-06-06 2008-06-06 Method of identifying improved protein versions

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US93331207P 2007-06-06 2007-06-06
US93333107P 2007-06-06 2007-06-06
US93330707P 2007-06-06 2007-06-06
US60/933,312 2007-06-06
US60/933,331 2007-06-06
US60/933,307 2007-06-06

Publications (2)

Publication Number Publication Date
WO2008153935A2 WO2008153935A2 (en) 2008-12-18
WO2008153935A3 true WO2008153935A3 (en) 2009-02-12

Family

ID=39820892

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/US2008/007114 WO2008153935A2 (en) 2007-06-06 2008-06-06 Methods for improving protein properties
PCT/US2008/007113 WO2008153934A2 (en) 2007-06-06 2008-06-06 Methods for improving protein performance
PCT/US2008/007103 WO2008153925A2 (en) 2007-06-06 2008-06-06 Methods for improving multiple protein properties

Family Applications After (2)

Application Number Title Priority Date Filing Date
PCT/US2008/007113 WO2008153934A2 (en) 2007-06-06 2008-06-06 Methods for improving protein performance
PCT/US2008/007103 WO2008153925A2 (en) 2007-06-06 2008-06-06 Methods for improving multiple protein properties

Country Status (11)

Country Link
US (4) US20100233780A1 (en)
EP (3) EP2171056B1 (en)
JP (4) JP5548613B2 (en)
KR (3) KR20100032367A (en)
CN (3) CN101778940B (en)
BR (3) BRPI0812206A2 (en)
CA (3) CA2690023C (en)
DK (3) DK2171055T3 (en)
MX (3) MX2009013083A (en)
RU (1) RU2553550C2 (en)
WO (3) WO2008153935A2 (en)

Cited By (4)

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US8916370B2 (en) 2008-04-23 2014-12-23 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
US9175313B2 (en) 2009-04-23 2015-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
US9273298B2 (en) 2010-10-27 2016-03-01 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene

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AU2013266516A1 (en) 2012-05-21 2014-10-09 Danisco Us Inc. Trichoderma hydrophobin production
BR112014031882A2 (en) 2012-06-20 2017-08-01 Novozymes As use of an isolated polypeptide, polypeptide, composition, isolated polynucleotide, nucleic acid construct or expression vector, recombinant expression host cell, methods for producing a polypeptide, for enhancing the nutritional value of an animal feed, and for the treatment of protein, use of at least one polypeptide, animal feed additive, animal feed, and detergent composition
CN104968211A (en) 2013-02-06 2015-10-07 诺维信公司 Polypeptides having protease activity
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CN108707595B (en) * 2018-07-03 2021-07-27 华东理工大学 Method for improving yield of cellulase produced by fungi
CN113284562B (en) * 2021-06-07 2021-12-24 中国农业科学院农业基因组研究所 Enzyme improvement method
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