WO2008121800A1 - Methods of separation and detection of bazedoxifene acetate in pharmaceutical compositions - Google Patents
Methods of separation and detection of bazedoxifene acetate in pharmaceutical compositions Download PDFInfo
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- WO2008121800A1 WO2008121800A1 PCT/US2008/058621 US2008058621W WO2008121800A1 WO 2008121800 A1 WO2008121800 A1 WO 2008121800A1 US 2008058621 W US2008058621 W US 2008058621W WO 2008121800 A1 WO2008121800 A1 WO 2008121800A1
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- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
- C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
- C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/12—Drugs for genital or sexual disorders; Contraceptives for climacteric disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/92—Chemical or biological purification of waste gases of engine exhaust gases
- B01D53/94—Chemical or biological purification of waste gases of engine exhaust gases by catalytic processes
- B01D53/9445—Simultaneously removing carbon monoxide, hydrocarbons or nitrogen oxides making use of three-way catalysts [TWC] or four-way-catalysts [FWC]
- B01D53/945—Simultaneously removing carbon monoxide, hydrocarbons or nitrogen oxides making use of three-way catalysts [TWC] or four-way-catalysts [FWC] characterised by a specific catalyst
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/12—Radicals substituted by oxygen atoms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02T—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO TRANSPORTATION
- Y02T10/00—Road transport of goods or passengers
- Y02T10/10—Internal combustion engine [ICE] based vehicles
- Y02T10/12—Improving ICE efficiencies
Definitions
- the disclosure relates to methods of separating and detecting apeledoxifene acetate from pharmaceutical compositions containing a mixture of apeledoxifene acetate and one or more other components. Methods of separating and detecting crystalline apeledoxifene acetate Form A and/or Form B in pharmaceutical compositions are disclosed.
- Bazedoxifene is an estrogenic agent which is useful in treating a variety of conditions, disorders or diseases, particularly those associated with menopause.
- Bazedoxifene salts, particularly, is used in pharmaceutical formulations.
- Bazedoxifene acetate (1-[4-(2-azepan-1-yl-ethoxy)-benzyl]-2-(4-hydroxy- phenyl)-3-methyl-1 H-indol-5-ol acetic acid) has the chemical formula shown below.
- Bazedoxifene belongs to the class of drugs typically referred to as selective estrogen receptor modulators (SERMs). Consistent with its classification, bazedoxifene demonstrates affinity for estrogen receptors (ER) but shows tissue selective estrogenic effects. Bazedoxifene acetate has demonstrated estrogenic activity on bone and cardiovascular lipid parameters and antiestrogenic activity on uterine and mammary tissue and thus has the potential for treating a number of different disease or disease-like states involving the estrogen receptor.
- SERMs selective estrogen receptor modulators
- U.S. Pat. Nos. 5,998,402 and 6,479,535 report the preparation of apeledoxifene acetate.
- the synthetic preparation of apeledoxifene acetate has also appeared in the general literature. See, for example, Miller et al., J. Med. Chem., 2001 , 44, 1654-1657. Description of the drug's biological activity has appeared in the general literature as well (e.g. Miller, et al. Drugs of the Future, 2002, 27(2), 1 17-121 ).
- pharmaceutical compositions containing apeledoxifene acetate is disclosed in WO 02/03987.
- the crystalline polymorph form of a particular drug often affects the drug's ease of preparation, stability, solubility, storage stability, ease of formulation and in vivo pharmacology.
- Polymorphic forms occur where the same composition of matter crystallizes in a different lattice arrangement resulting in different thermodynamic properties and stabilities specific to the particular polymorph form.
- Polymorphic Form A of apeledoxifene acetate is disclosed in US 2005/0227965 while polymorphic Form B of apeledoxifene acetate is disclosed in US 2005/0250762.
- Methods of preparing polymorphic Form A of apeledoxifene acetate are also disclosed in commonly assigned and co-pending Patent Application Serial Nos. 61/027,607 and 61/027,634, filed on February 1 1 , 2008.
- Form A has higher solubility in both aqueous and organic solvent systems than Form B. This can be advantageous in formulations or doses where the solubility of the particular composition is of concern.
- Form A is the kinetic (or meta-stable) polymorph while Form B is the thermodynamically more stable polymorph.
- Form A can easily convert to Form B upon contact with a solvent or solvent mixture (e.g., ethyl acetate and ethanol), which presents a challenge to the preparation of pure Form A that is substantially free of Form B. Further, under various conditions, and over time, Form A can convert to Form B.
- a solvent or solvent mixture e.g., ethyl acetate and ethanol
- the detection limits for Form B are estimated at about 10% by weight relative to total apeledoxifene acetate for tablets containing 45.1 mg of apeledoxifene acetate (40 mg of apeledoxifene as free base) and 20% by weight relative to total apeledoxifene acetate for tablets containing 22.6mg of apeledoxifene acetate (20 mg of apeledoxifene as free base), based on apeledoxifene acetate weight percentage in the tablets and instrument noise levels. If interfering excipients, such as lactose and sucrose, can be removed while crystalline apeledoxifene acetate Form A and Form B are retained, Form B can, be detected with greater sensitivity.
- separating and detecting crystalline cryptedoxifene acetate Form A and/or Form B in pharmaceutical compositions are provided.
- methods are provided for separating a pharmaceutical composition comprising apeledoxifene acetate and one or more components that produce X- Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate. The method includes:
- the method further comprises washing the filtrand.
- the method further comprises forming the composition obtained in step (c) mentioned above into a tablet or pellet for X-Ray diffraction measurement.
- the apeledoxifene acetate is apeledoxifene acetate Form
- the characteristic peak for apeledoxifene acetate Form A is at about 12.8 ⁇ 0.2° in 2theta angular degree by Cu radiation, and the characteristic peaks for apeledoxifene acetate Form B are at about 12.0 ⁇ 0.2° and
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, pharmaceutically acceptable diluents, fillers, excipients, binding agents, lubricants, disintegrants, suspending or stabilizing agents, and mixtures thereof.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include lactose, sucrose, or mixtures thereof.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include lactose.
- the interfering peaks are at from about 1 1.6 ⁇ 0.2° to about 13.7 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the extraction medium is a solution including one or more acetate salts.
- the solution includes ammonium acetate, sodium acetate, potassium acetate, magnesium acetate, calcium acetate, or mixtures thereof.
- the solution comprises ammonium acetate, sodium acetate, or mixtures thereof. In some cases, the solution comprises sodium acetate.
- the solution has a concentration of about 0.05 M to about 1 M with respect to acetate. In certain embodiments, the solution has a concentration of about 0.25 M to about 0.75 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.45 M to about 0.55 M with respect to acetate.
- the solution has a pH range from about 5 to about 10. In certain embodiments, the solution has a pH range from about 6 to about 9.2. In some embodiments, the solution has a pH range from about 6.2 to about 8.5.
- the pharmaceutical composition is provided as at least one unit dosage form.
- unit dosage forms include, without limitation, tablet, capsule, gel cap, buccal form, troche, and lozenge.
- the unit dosage form is tablet.
- the method further comprises removing any coating from the pharmaceutical composition prior to contacting the composition with the extraction medium.
- the amount of the extraction medium used during the contacting of the pharmaceutical composition with the extraction medium to produce the suspension is from about 0.2 ml per dosage (e.g., tablet) unit to about 10 ml per dosage unit.
- the pharmaceutical composition is contacted with the extraction medium for about 1 to about 120 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 5 to about 30 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 5 to about 15 minutes.
- the method includes:
- the pharmaceutical composition is provided as tablet form, and the method further includes removing any coating from the tablet prior to contacting the tablet with the solution.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, lactose, sucrose, and mixtures thereof.
- the characteristic peak for apeledoxifene acetate Form A is at about 12.8 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the characteristic peaks for apeledoxifene acetate Form B are at about 12.0 ⁇ 0.2° and about 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the interfering peaks are at from about 1 1.6 ⁇ 0.2° to about 13.7 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- a method for detecting apeledoxifene acetate Form A and/or Form B in a pharmaceutical composition comprising apeledoxifene acetate Form A and/or Form B and one or more components (e.g., lactose) that produce X-Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate Form A and/or Form B.
- the method includes:
- a method for separating a pharmaceutical composition comprising acipient, a drug, and one or more components that produce X- Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate.
- the method includes:
- the pharmaceutical composition further includes conjugated estrogens.
- the pharmaceutical composition includes a core containing conjugated estrogens and an outer layer containing apeledoxifene acetate.
- the contacting of the pharmaceutical composition with an extraction medium in step (a) is stopped when a filler coating between the conjugated estrogen core and the apeledoxifene outer layer is exposed.
- the method further comprises removing the supernatant solution produced in step (b). In some instances, the supernatant solution can be removed by decanting or through pipette. In some embodiments, the method further includes washing the solid produced in step (b).
- the solid is collected by filtration.
- the method further comprises forming the composition obtained in step (c) into powder or a tablet or pellet for X-Ray diffraction measurement.
- the method further includes analyzing the composition obtained in step (c) or the powder, tablet or pellet prepared from the composition using X-Ray diffraction.
- the apeledoxifene acetate is apeledoxifene acetate Form
- the characteristic peak for apeledoxifene acetate Form A is at about 12.8 ⁇ 0.2° in 2theta angular degree by Cu radiation
- the characteristic peaks for apeledoxifene acetate Form B are at about 12.0 ⁇ 0.2° and about 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, pharmaceutically acceptable diluents, fillers, excipients, binding agents, lubricants, disintegrants, suspending or stabilizing agents, and mixtures thereof.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, lactose, sucrose, or mixtures thereof.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, sucrose.
- the interfering peaks are at from about 1 1.9 ⁇ 0.2° to about 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the extraction medium is a solution comprising one or more acetate salts.
- the solution includes ammonium acetate, sodium acetate, potassium acetate, magnesium acetate, calcium acetate, or mixtures thereof.
- the solution includes ammonium acetate, sodium acetate, or mixtures thereof.
- the solution has a concentration of about 0.05 M to about 1 M with respect to acetate. In certain embodiments, the solution has a concentration of about 0.1 M to about 0.75 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.1 M to about 0.3 M with respect to acetate. [0038] In certain embodiments, the solution has a pH between about 5 and about 10. In certain embodiments, the solution has a pH between about 6 and about 9.2. In some embodiments, solution has a pH between about 6.2 and about 8.5. [0039] In certain embodiments, the pharmaceutical composition is provided as at least one unit dosage form.
- Non-limiting examples of the unit dosage form include tablet, capsule, gel cap, buccal form, troche, and lozenge.
- the pharmaceutical composition is provided as a tablet.
- the tablet form includes a core and an outer layer.
- the core contains conjugated estrogens and the outer layer contains apeledoxifene acetate.
- the method further includes removing any coating from the pharmaceutical composition prior to contacting the composition with the extraction medium.
- the amount of the extraction medium used during the contacting of the pharmaceutical composition with the extraction medium to produce the suspension is from about 0.2 ml per dosage unit to about 10 ml per dosage unit (e.g., 10mg, 20mg or 40mg apelidoxifene tablet, optionally containing conjugated estrogens).
- the pharmaceutical composition is contacted with the extraction medium for about 1 to about 120 minutes.
- the pharmaceutical composition is contacted with the extraction medium for about 1 to about 30 minutes.
- the pharmaceutical composition is contacted with the extraction medium for about 1 to about 5 minutes.
- the pharmaceutical composition is contacted with the extraction medium for about 2 minutes.
- a method for separating a pharmaceutical composition comprising acipient, a drug, and/or Form B and one or more components that produce X-Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate Form A and/or Form B.
- the method includes:
- the pharmaceutical composition is provided as a tablet.
- the tablet includes a core and an outer layer.
- the core contains conjugated estrogens and the outer layer contains apeledoxifene acetate Form A and/or Form B.
- the method further includes removing any coating from the tablet prior to contacting the tablet with the solution.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include lactose, sucrose, or mixtures thereof.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include sucrose.
- the characteristic peak for apeledoxifene acetate Form A is at about 12.8 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the characteristic peaks for apeledoxifene acetate Form B are at about 12.0 ⁇ 0.2° and 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the interfering peaks are at from about 1 1.9 ⁇ 0.2° to about 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- a method for detecting apeledoxifene acetate Form A and/or Form B in a pharmaceutical composition comprising acipient, alumedoxifene acetate Form A and/or Form B and one or more components (e.g., sucrose) that produce X-Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate Form A and/or Form B.
- the method includes:
- Figure 1 shows the X-Ray diffraction (XRD) patterns of acetate
- Figure 2 shows the XRD patterns of apeledoxifene acetate Forms A and B, and sucrose.
- One aspect provides methods of separating a pharmaceutical composition comprising apeledoxifene acetate and one or more components that produce X-Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate.
- the method includes: (a) contacting the pharmaceutical composition with an extraction medium to produce a suspension, in which apeledoxifene acetate is substantially insoluble in the extraction medium and in which the one or more components are substantially soluble in the extraction medium; (b) filtering the suspension to produce a filtrate and a filtrand, in which the one or more components are substantially contained in the filtrate; and (c) drying the filtrand to produce a composition substantially free of the one or more components that produce X-Ray diffraction patterns having one or more interfering peaks.
- the bazedoxifene acetate is substantially contained in the filtrand.
- the method further includes washing the filtrand.
- the method further includes forming the composition obtained in step (c) mentioned above into powder or tablet or pellet for X-Ray diffraction measurement.
- the apeledoxifene acetate is apeledoxifene acetate Form A and/or apeledoxifene acetate Form B. In some cases, the apeledoxifene acetate is a mixture of apeledoxifene acetate Form A and apeledoxifene acetate Form B.
- the characteristic peak for apeledoxifene acetate Form A is at about 12.8 ⁇ 0.2° in 2theta angular degree by Cu radiation
- the characteristic peaks for apeledoxifene acetate Form B are at about 12.0 ⁇ 0.2° and about 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the one or more components (“interfering components”) that produce X-Ray diffraction patterns having interfering peaks include, without limitation, pharmaceutically acceptable diluents, fillers, excipients, binding agents, lubricants, disintegrants, suspending or stabilizing agents, and mixtures thereof.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, but are not limited to, one or more of magnesium stearate, stearic acid, talc, sodium lauryl sulfate, microcrystalline cellulose, ascorbic acid, sodium starch glycolate, pregelled starch, carboxymethylcellulose calcium, polyvinylpyrrolidone, gelatin, alginic acid, acacia gum, xanthan gum, sodium citrate, complex silicates, calcium carbonate, glycine, dextrin, sucrose, sorbitol, dicalcium phosphate, calcium sulfate, lactose, kaolin, mannitol, sodium chloride, dry starches and powdered sugar.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, lactose, sucrose, or mixtures thereof. In some embodiments, the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, lactose. In certain embodiments, the interfering peaks are at from about 1 1.6 ⁇ 0.2° to about 13.7 ⁇ 0.2° in 2theta angular degree by Cu radiation. [0053] Figure 1 illustrates the interference with the characteristic peaks of apeledoxifene acetate Forms A and B caused by lactose.
- the peak of lactose at about 12.5 ⁇ 0.2° interferes with the characteristic peak of apeledoxifene acetate Form A at about 12.8 ⁇ 0.2° and the characteristic peaks of apeledoxifene acetate Form B at about 12.0 ⁇ 0.2°.
- the interfering peaks are at from about 1 1.9 ⁇ 0.2° to about 13.3 ⁇ 0.2°.
- Figure 2 illustrates the interference with the characteristic peaks of apeledoxifene acetate Forms A and B caused by sucrose.
- the peak of sucrose at about 12.9 ⁇ 0.2° interferes with the characteristic peak of apeledoxifene acetate Form A at about 12.8 ⁇ 0.2°.
- the peak of sucrose at about 1 1.9 ⁇ 0.2° and 13.3 ⁇ 0.2° interferes with the characteristic peak of apeledoxifene acetate Form B at about 12.0 ⁇ 0.2° and 13.3 ⁇ 0.2° respectively.
- substantially insoluble means “sparingly soluble,” “slightly soluble,” “very slightly soluble,” or “practically insoluble, or insoluble” as described in USP 25, The United States Pharmacopeia, page 2363 (2002).
- the term "substantially insoluble" with respect to apeledoxifene acetate means that less than about 1 mg of apeledoxifene acetate can dissolve in one ml_ of extraction solution, for example, less than about 0.5 mg of apeledoxifene acetate can dissolve in one ml_ of extraction solution, less than about 0.1 mg of apeledoxifene acetate can dissolve in one ml_ of extraction solution, less than about 0.05 mg of apeledoxifene acetate can dissolve in one ml_ of extraction solution, less than about 0.01 mg of apeledoxifene acetate can dissolve in one ml_ of extraction solution, or less than about 0.005 mg of apeledoxifene acetate can dissolve in one ml_ of extraction solution.
- the term “substantially soluble” as used herein means “very soluble,” “freely soluble,” or “soluble” as described in USP 25, The United States Pharmacopeia, page 2363 (2002).
- the term “substantially soluble” as used herein with respect to an interfering component means that greater than about 20 mg of the interfering component can dissolve in one ml_ of extraction solution, for example, greater than about 50 mg of the interfering component can dissolve in one ml_ of extraction solution, greater than about 100 mg of the interfering component can dissolve in one ml_ of extraction solution, greater than about 200 mg of the interfering component can dissolve in one ml_ of extraction solution, greater than about 350 mg of the interfering component can dissolve in one ml_ of extraction solution, greater than about 500 mg of the interfering component can dissolve in one ml_ of extraction solution, greater than about 1000 mg of the interfering component can dissolve in one ml_ of extraction solution, greater than about 1500 mg of the interfer
- Bazedoxifene acetate or one or more other components is identified as substantially contained in, e.g., a filtrand, filtrate, or supernatant solution or solid produced by centrifugation.
- substantially contained means that at least about 70% by weight of the identified apeledoxifene acetate or other component is contained in the specified filtrate, filtrand, supernatant solution or solid compared to the amount of the corresponding component contained in the initial pharmaceutical composition being analyzed. In some instances, at least about 80% or at least 90% of the identified apeledoxifene acetate or other component is contained in the specified filtrate, filtrand, supernatant solution or solid.
- the term "substantially free” as used herein means that the interfering component makes up no more than about 25% by weight of the final composition as prepared according to a method described herein. In certain embodiments, the interfering component makes up no more than about 20%, about 10%, about 5%, or about 1 % by weight of the final composition.
- the extraction medium is a solution comprising one or more acetate salts.
- the solution includes ammonium acetate, sodium acetate, potassium acetate, magnesium acetate, calcium acetate, or mixtures thereof.
- the solution includes ammonium acetate, sodium acetate, or mixtures thereof.
- the solution includes sodium acetate.
- Certain embodiments provide methods to extract apeledoxifene acetate from formulations, without altering the apeledoxifene acetate crystal form. While not to be bound by theory, it is believed that high concentrations of acetate (Ac ) may suppress the dissociation and dissolution of apeledoxifene acetate.
- the solution has a concentration of about 0.05 M to about 1 M with respect to acetate. In certain embodiments, the solution has a concentration of about 0.1 M to about 0.9 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.15 M to about 0.85 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.2 M to about 0.8 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.25 M to about 0.75 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.3 M to about 0.7 M with respect to acetate.
- the solution has a concentration of about 0.35 M to about 0.65 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.4 M to about 0.6 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.45 M to about 0.55 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.5 M with respect to acetate.
- the extraction medium has a pH of about 8.2 to about 8.5. In some embodiments, the extraction medium has a pH of about 8.3 to about 8.4. [0063] In some embodiments, the extraction medium includes a solution of ammonium acetate. In some embodiments, the extraction medium includes about 0.5 M ammonium acetate. In some embodiments, the extraction medium includes about 0.5 M ammonium acetate and has a pH of about 6.2.
- the extraction medium includes a mixture of ammonium acetate and sodium acetate. In some embodiments, the extraction medium includes a solution of: about 0.05 M to about 0.5 M ammonium acetate; and about 0.05 M to about 0.5 M sodium acetate. In some embodiments, the extraction medium includes ammonium acetate and sodium acetate and has a pH of about 6.5 to about 7.5. [0065] In some embodiments, the extraction medium includes about 0.1 M ammonium acetate and about 0.4 M sodium acetate. In some embodiments, the extraction medium includes about 0.15 M ammonium acetate and about 0.35 M sodium acetate.
- the extraction medium includes about 0.2 M ammonium acetate and about 0.3 M sodium acetate. In some embodiments, the extraction medium includes about 0.25 M ammonium acetate and about 0.25 M sodium acetate. In some embodiments, the extraction medium includes about 0.3 M ammonium acetate and about 0.2 M sodium acetate. In some embodiments, the extraction medium includes about 0.35 M ammonium acetate and about 0.15 M sodium acetate. In some embodiments, the extraction medium includes about 0.4 M ammonium acetate and about 0.1 M sodium acetate. In some embodiments, the extraction medium includes about 0.45 M ammonium acetate and about 0.05 M sodium acetate.
- the extraction medium includes about 0.125 M ammonium acetate and about 0.375 M sodium acetate. In some embodiments, the extraction medium includes about 0.125 M ammonium acetate and about 0.375 M sodium acetate has a pH of about 6.85. In some embodiments, the extraction medium includes about 0.05 M ammonium acetate and about 0.45 M sodium acetate. In some embodiments, the extraction medium includes about 0.05 M ammonium acetate and about 0.45 M sodium acetate has a pH of about 7.18.
- the extraction medium includes sodium acetate. In some embodiments, the extraction medium includes about 0.5 M sodium acetate. In some embodiments, the extraction medium includes about 0.5 M sodium acetate and has a pH of about 8.34.
- the pharmaceutical composition is provided as at least one unit dosage form.
- unit dosage forms include, without limitation, tablet, capsule, gel cap, buccal form, troche, and lozenge.
- the unit dosage form is a tablet.
- the dosage units described herein can utilize standard delay or time release formulations or spansules.
- any coating is removed from the dosage unit prior to contacting it with the extraction medium.
- the pharmaceutical dosage unit is broken apart prior to and/or during mixing with the extraction medium.
- the amount of the extraction medium used during the contacting of the pharmaceutical composition with the extraction medium to produce the suspension is from about 0.2 ml per dosage unit to about 10 ml per dosage unit (e.g., tablet). In some embodiments, the amount of the extraction medium used during the mixing of the pharmaceutical composition and the extraction medium is about 0.8 ml per dosage unit. In some embodiments, the amount of the extraction medium used during the mixing of the pharmaceutical composition and the extraction medium is about 1 ml per dosage unit.
- the amount of the extraction medium used during the mixing of the pharmaceutical composition and the extraction medium is about 1.67 ml per dosage unit. In some embodiments, the amount of the extraction medium used during the mixing of the pharmaceutical composition and the extraction medium is about 2.5 ml per dosage unit. In some embodiments, the amount of extraction medium used is inversely related to the molar concentration of acetate ion in the extraction medium.
- the pharmaceutical composition is contacted with the extraction medium for about 1 to about 120 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 5 to about 30 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 5 to about 10 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 2 to about 5 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 2 minutes.
- a mixture of the pharmaceutical composition and the extraction medium is filtered to produce a filtrand. In some instances, washing is performed with additional extraction medium.
- the filtrand is dried. In some embodiments, the filtrand is dried overnight. In some embodiments, the filtrand is dried at an elevated temperature, for example, at about 40 0 C. In some embodiments, the filtrand is dried at an elevated temperature, for example, at about 40 0 C overnight. In some embodiments, the filtrand is dried at room temperature. In some embodiments, the filtrand is dried under vacuum. In some embodiments, the filtrand is dried under vacuum and at an elevated temperature, for example, at about 40°C.
- Another aspect provides methods of separating a pharmaceutical composition comprising apeledoxifene acetate Form A and/or Form B and one or more components (e.g., lactose) that produce X-Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate Form A and/or Form B.
- components e.g., lactose
- the method includes: (a) contacting the pharmaceutical composition with a solution comprising at least one acetate salt to produce a suspension, in which the apeledoxifene acetate Form A and/or Form B is substantially insoluble in the solution and in which the one or more components (e.g., lactose) are substantially soluble in the solution; (b) filtering the suspension to produce a filtrate and a filtrand, in which the one or more components are substantially contained in the filtrate; and (c) washing and drying the filtrand to produce a composition substantially free of the one or more components (e.g., lactose) that produce X- Ray diffraction patterns having one or more interfering peaks.
- a solution comprising at least one acetate salt
- the one or more components e.g., lactose
- the pharmaceutical composition is provided as tablet form, and the method further includes removing any coating from the tablet prior to contacting the tablet with the solution.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, lactose, sucrose, or mixtures thereof.
- the characteristic peak for apeledoxifene acetate Form A is at about 12.8 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the characteristic peaks for apeledoxifene acetate Form B are at about 12.0 ⁇ 0.2° and 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the interfering peaks are at from about 1 1.6 ⁇ 0.2° to about 13.7 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- Yet another aspect provides a method of detecting apeledoxifene acetate Form A and/or Form B in a pharmaceutical composition comprising apeledoxifene acetate Form A and/or Form B and one or more components that produce X-Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate Form A and/or Form B.
- the method includes: producing a composition containing apeledoxifene acetate Form A and/or Form B by a method as described hereinabove, wherein the composition is substantially free of the one or more components that produce X- Ray diffraction patterns having one or more interfering peaks; forming the composition containing apeledoxifene acetate Form A and/or Form B into powder or tablet or pellet for X- Ray diffraction measurement; and analyzing the powder or tablet or pellet using X-Ray diffraction.
- the method includes: (a) contacting the pharmaceutical composition with an extraction medium to produce a suspension, in which the apeledoxifene acetate is substantially insoluble in the extraction medium and in which the one or more components are substantially soluble in the extraction medium; (b) centrifuging the suspension to produce a solid and a supernatant solution, in which the one or more components are substantially contained in the supernatant solution; and (c) collecting and drying the solid to produce a composition substantially free of the one or more components that produce X-Ray diffraction patterns having one or more interfering peaks.
- the pharmaceutical composition further includes conjugated estrogens.
- the pharmaceutical composition includes a core containing conjugated estrogens and an outer layer containing apeledoxifene acetate.
- the contacting of the pharmaceutical composition with an extraction medium in step (a) is stopped when a filler coating between the conjugated estrogen core and the apeledoxefene outer layer is exposed.
- the apeledoxifene acetate is substantially contained in the solid produced by centrifugation.
- step (b) is repeated as needed.
- the method further includes removing the supernatant solution provided in step (b).
- the supernatant solution can be removed by decanting or through pipette.
- the method further includes washing the solid provided in step (b).
- the solid is collected by filtration.
- the method further includes forming the composition obtained in step (c) into powder or a tablet or pellet for X-Ray diffraction measurement.
- the method further includes analyzing the composition obtained in step (c) or the powder, tablet or pellet prepared from the composition using X-Ray diffraction.
- the apeledoxifene acetate is apeledoxifene acetate Form A and/or apeledoxifene acetate Form B.
- the characteristic peak for apeledoxifene acetate Form A is at about 12.8 ⁇ 0.2° in 2theta angular degree by Cu radiation
- the characteristic peaks for apeledoxifene acetate Form B are at about 12.0 ⁇ 0.2° and about 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include, without limitation, pharmaceutically acceptable diluents, fillers, excipients, binding agents, lubricants, disintegrants, suspending or stabilizing agents, and mixtures thereof.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include lactose, sucrose, or mixtures thereof.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include sucrose.
- the interfering peaks are at from about 1 1.9 ⁇ 0.2° to about 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the extraction medium is a solution comprising one or more acetate salts.
- the solution includes ammonium acetate, sodium acetate, potassium acetate, magnesium acetate, calcium acetate, or mixtures thereof.
- the solution includes ammonium acetate, sodium acetate, or mixtures thereof.
- the solution has a concentration of about 0.05 M to about 1 M with respect to acetate. In certain embodiments, the solution has a concentration of about 0.1 M to about 0.9 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.15 M to about 0.85 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.2 M to about 0.8 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.25 M to about 0.75 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.3 M to about 0.7 M with respect to acetate.
- the solution has a concentration of about 0.35 M to about 0.65 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.4 M to about 0.6 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.45 M to about 0.55 M with respect to acetate. In some embodiments, the solution has a concentration of about 0.5 M with respect to acetate. [0088] In some embodiments, the extraction medium has a pH of about 1 to about 13. In some embodiments, the extraction medium has a pH of about 5 to about 12. In certain embodiments, the extraction medium has a pH of about 5 to about 10. In some embodiments, the extraction medium has a pH of about 6 to about 1 1.
- the extraction medium has a pH of about 6.5 to about 10.5. In certain embodiments, the extraction medium has a pH of about 6 to about 9.2. In some embodiments, the extraction medium has a pH of about 6.2 to about 8.5. In some embodiments, the extraction medium has a pH of about 7 to about 10. In some embodiments, the extraction medium has a pH of about 7.5 to about 9.5. In some embodiments, the extraction medium has a pH of about 8 to about 9. In some embodiments, the extraction medium has a pH of about 8.2 to about 8.5. In some embodiments, the extraction medium has a pH of about 8.3 to about 8.4. [0089] In certain embodiments, the pharmaceutical composition is provided as at least one unit dosage form.
- Non-limiting examples of the unit dosage form include tablet, capsule, gel cap, buccal form, troche, and lozenge.
- the pharmaceutical composition is provided as a tablet.
- the tablet includes a core and an outer layer.
- the core contains conjugated estrogens and the outer layer contains apeledoxifene acetate.
- the amount of the extraction medium used during the contacting of the pharmaceutical composition with the extraction medium to produce the suspension is from about 0.2 ml per dosage unit (e.g., tablet) to about 10 ml per dosage unit. In some embodiments, the amount of the extraction medium used during the mixing of the pharmaceutical composition and the extraction medium is about 0.8 ml per dosage unit. In some embodiments, the amount of the extraction medium used during the mixing of the pharmaceutical composition and the extraction medium is about 1 ml per dosage unit. In some embodiments, the amount of the extraction medium used during the mixing of the pharmaceutical composition and the extraction medium is about 1.67 ml per dosage unit.
- the amount of the extraction medium used during the mixing of the pharmaceutical composition and the extraction medium is about 2.5 ml per dosage unit. In some embodiments, the amount of extraction medium used is inversely related to the molar concentration of acetate ion in the extraction medium.
- the pharmaceutical composition is contacted with the extraction medium for about 1 to about 120 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 5 to about 30 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 5 to about 10 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 2 to about 5 minutes. In some embodiments, the pharmaceutical composition is contacted with the extraction medium for about 2 minutes.
- the method includes: (a) contacting the pharmaceutical composition with a solution comprising at least one acetate salt to produce a suspension, wherein apeledoxifene acetate Form A and/or Form B is substantially insoluble in the solution and wherein the one or more components are substantially soluble in the solution; (b) centrifuging the suspension to produce a solid and a supernatant solution, wherein the one or more components are substantially contained in the supernatant solution; and (c) collecting and drying the solid to produce a composition substantially free of the one or more components that produce X-Ray diffraction patterns having one or more interfering peaks.
- the pharmaceutical composition is provided as a tablet.
- the tablet includes a core and an outer layer.
- the core contains conjugated estrogens and the outer layer contains apeledoxifene acetate Form A and/or Form B.
- the method further includes removing any coating from the tablet prior to contacting the tablet with the solution.
- the one or more components that produce X-Ray diffraction patterns having interfering peaks include lactose, sucrose, or mixtures thereof. In certain embodiments, the one or more components that produce X-Ray diffraction patterns having interfering peaks include sucrose.
- the characteristic peak for apeledoxifene acetate Form A is at about 12.8 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the characteristic peaks for apeledoxifene acetate Form B are at about 12.0 ⁇ 0.2° and 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- the interfering peaks are at from about 1 1.9 ⁇ 0.2° to about 13.3 ⁇ 0.2° in 2theta angular degree by Cu radiation.
- a further aspect provides a method of detecting apeledoxifene acetate Form A and/or Form B in a pharmaceutical composition comprising apeledoxifene acetate Form A and/or Form B and one or more components (e.g., sucrose) that produce X-Ray diffraction patterns having one or more interfering peaks at or near the characteristic peak or peaks for apeledoxifene acetate Form A and/or Form B.
- the method includes:
- the methods described herein includes: preparing a final composition that is substantially free of interfering components (e.g., lactose or sucrose) for XRD analysis.
- the final composition is ground and pressed into a tablet or pellet for XRD measurement.
- the analysis of the final composition is carried out using a Phillips X-Pert PW3040-MPD diffractometer.
- the analysis of the final composition is carried out using a Bruker D8 Discover X-ray diffractometer with GADDS.
- the embodiments of the methods described above can be combined in any manner.
- features from one embodiment can be combined with features from any other embodiment.
- the embodiments described above can be combined in a manner to produce methods of detecting apeledoxifene acetate Form A and/or Form B in a pharmaceutical composition comprising apeledoxifene acetate and one or more components that produce XRD patterns having interfering peaks at or near the characteristic peaks of apeledoxifene acetate Form A and/or Form B, comprising: removing any coating from at least one dosage unit comprising apeledoxifene acetate and one or more pharmaceutically acceptable diluents, fillers, excipients, binding agents, lubricants, disintegrants, or suspending or stabilizing agents (e.g., lactose); adding sodium acetate solution (about 0.5 M) to the pharmaceutical composition to produce a suspension with stirring for about 2 to about 15 minutes; filtering and washing the suspension with additional sodium acetate solution to produce a filtr
- the embodiments described above can be combined in a manner to produce methods of detecting apeledoxifene acetate Form A and/or Form B in a pharmaceutical composition comprising apeledoxifene acetate and one or more components that produce XRD patterns having interfering peaks at or near the characteristic peaks of apeledoxifene acetate Form A and/or Form B.
- the method includes: removing any coating from at least one dosage unit comprising apeledoxifene acetate and one or more pharmaceutically acceptable diluents, fillers, excipients, binding agents, lubricants, disintegrants, or suspending or stabilizing agents (e.g., sucrose); adding an appropriate amount of sodium acetate solution (about 0.2 M) to the pharmaceutical composition to produce a suspension with stirring for about 2 to about 10 minutes; centrifuging the suspension to produce a solid and a supernatant solution; removing the supernatant solution; adding additional sodium acetate solution to the solid to produce another suspension with shaking; centrifuging the suspension to produce another solid and another supernatant solution; removing the supernatant solution; filtering and drying the solid; grinding and pressing the solid into pellets for XRD measurement; and analyzing the prepared pellets using an XRD diffractometer, e.g., Bruker D8 Discover X-ray diffractometer with GADDS.
- the methods disclosed according to certain embodiments herein advantageously allow for detecting apeledoxifene acetate Form B that is present in a pharmaceutical composition in low levels. For example, about 2% by weight of apeledoxifene acetate Form B relative to the total apeledoxifene acetate can be detected after the application of the extraction procedure as described according to certain embodiments herein due to the increase of the detectable apeledoxifene acetate signals in X-Ray diffraction upon removal of interfering component(s).
- Tablets used herein comprise BZA Form A, lactose monohydrate, ascorbic acid, microcrystalline cellulose, pregelatinized starch, sodium starch glycolate, colloidal silicon dioxide, magnesium stearate, and sodium lauryl sulfate.
- the tablets further comprise a coating containing Opadry® White and Opadry® Clear.
- Tablets used herein comprise a core comprising CE, lactose monohydrate, microcrystalline cellulose, and hypromellose; an outer layer conmprising BZA Form A, ascorbic acid, sucrose, hypromellose, and sucrose palmitic acid ester; and a filler coat between the core and the outer layer, which filler coat containing sucrose, microcrystalline cellulose, hypromellose, polyethylene glycol.
- the tablets further comprise a coating containing Opadry® Pink and Opadry® 2 Clear.
- the coatings of twenty of the BZA/CE tablets were removed by razor blades or other appropriate means.
- the resulting tablets were placed in a 50 ml beaker.
- About 20-25 ml of 0.2 M sodium acetate solution was added to the beaker to produce a mixture.
- the mixture was stirred with a magnetic stirring bar to produce a suspension.
- the resulting suspension was transferred into a 50 ml centrifuge tube.
- About another 10-25 ml of 0.2 M sodium acetate solution was added to the beaker containing residual tablets to produce a mixture.
- the mixture was stirred to produce a suspension, which was again transferred into the same centrifuge tube. (This step may be repeated as needed until a filler coating between the CE core and the BZA outer layer was exposed.
- the filler coating between the BZA outer layer and the CE core usually looks smooth and whitish.
- the resulting suspension was centrifuged to produce a supernatant solution and a solid. The supernatant solution was removed. The solid remained in the centrifuge tube was added about 40 ml of 0.2 M sodium acetate, then shaken, centrifuged and the resulting supernatant solution removed again. (This step may be repeated as needed.) The remaining solid in the centrifuge tube was filtered to remove any leftover extraction medium. The recovered solid produced by filtration was dried at about 40 0 C overnight (12 to 18 hours) . [0107] The dried solid was grinded and pressed into pellets using an IR hydraulic presser for XRD measurement.
- X-Ray Powder Diffraction analyses were carried out on samples prepared according to Examples 1 and 2 using a Bruker D8 Discover X-ray diffractometer with GADDS.
- the diffractometer power was set at 40 kV and 40 mA.
- the collimator diameter of the instrument was about 0.8 mm and the detector-to-sample distance was set at about 30 cm.
- the X-ray incident angle relative to the pellet/tablet surface was about 4° and the detection angle relative to the pellet/tablet surface was about 16 ⁇ 0.2°. Data were collected at about 120 minutes to about 240 minutes.
- X-Ray Diffraction analyses on powder samples can be carried out on a (Philips X'Pert MPD) X-ray diffractometer using Cu radiation x-ray beams.
- the diffractometer power was set at 40 kV and 40 mA.
- a continuous scan at 0.02 degree/second from 4 to 40 ° was used.
- Figure 1 and 2 show the XRD patterns collected from apeledoxifene acetate Form A and Form B, lactose and sucrose powders.
- a calibration curve between the apeledoxifene concentration and the UV peak area at 220nm was established from these standard apeledoxifene acetate solutions.
- Bazedoxifene acetate concentration of a given sample is determined by its UV peak area at 220nm using the calibration curve .
- the identification of apeledoxifene by HPLC is determined by comparing the retention time of the apeledoxifene peak in the sample preparation chromatogram to that of the apeledoxifene peak in the standard preparation.
- Example 1 The following table illustrates the results of experiments using the extraction methods of Example 1. The extractions were performed using different extraction media and the results were measured in terms of peak area of apeledoxifene using XRD as described in Example 3. As can be seen from Table 1 , the extraction medium with the highest recovery rate for this particulare set of experiments was 0.50 M Sodium Acetate, at a pH of about 8.34.
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Priority Applications (7)
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BRPI0809950-2A2A BRPI0809950A2 (en) | 2007-03-30 | 2008-03-28 | SEPARATION AND DETECTION METHODS OF BAZEDOXYPHEN ACETATE IN PHARMACEUTICAL COMPOSITIONS |
AU2008232666A AU2008232666A1 (en) | 2007-03-30 | 2008-03-28 | Methods of separation and detection of bazedoxifene acetate in pharmaceutical compositions |
JP2010502214A JP2010523982A (en) | 2007-03-30 | 2008-03-28 | Method for separating and detecting bazedoxifene acetate in pharmaceutical compositions |
CA002682185A CA2682185A1 (en) | 2007-03-30 | 2008-03-28 | Methods of separation and detection of bazedoxifene acetate in pharmaceutical compositions |
MX2009010508A MX2009010508A (en) | 2007-03-30 | 2008-03-28 | Methods of separation and detection of bazedoxifene acetate in pharmaceutical compositions. |
EP08744576A EP2132172A1 (en) | 2007-03-30 | 2008-03-28 | Methods of separation and detection of bazedoxifene acetate in pharmaceutical compositions |
IL201043A IL201043A0 (en) | 2007-03-30 | 2009-09-17 | Methods of separation and detection of bazedoxifene acetate in pharmaceutical compositions |
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US90911307P | 2007-03-30 | 2007-03-30 | |
US60/909,113 | 2007-03-30 |
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US (1) | US20080249303A1 (en) |
EP (1) | EP2132172A1 (en) |
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CN (1) | CN101657421A (en) |
AR (1) | AR065875A1 (en) |
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WO2012156450A2 (en) * | 2011-05-17 | 2012-11-22 | Zach System S.P.A. | Method of detecting polymorphs using synchrotron radiation |
CN106198827B (en) * | 2016-08-11 | 2018-03-27 | 齐鲁制药有限公司 | The efficiently Pharmaceutical Analysis method of measure bazedoxifene acetate and its impurity |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4101511A (en) * | 1976-08-05 | 1978-07-18 | Exxon Research & Engineering Co. | Novel bisphenol derivatives and uses thereof |
EP0263902A1 (en) * | 1985-06-26 | 1988-04-20 | The Upjohn Company | Solubilisation and oxidation of somatotropin from transformed microorganisms |
WO1991006005A1 (en) * | 1989-10-19 | 1991-05-02 | Yamasa Shoyu Kabushiki Kaisha | Method of assaying d-vanillylmandelic acid, and reagent and kit therefor |
WO2000071142A1 (en) * | 1999-05-20 | 2000-11-30 | Alpha Therapeutic Corporation | Method for preparing dual virally inactivated immune globulin for intravenous administration |
WO2003087796A1 (en) * | 2002-04-10 | 2003-10-23 | Bristol-Myers Squibb Company | High throughput x-ray diffraction filter sample holder |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5998402A (en) * | 1996-04-19 | 1999-12-07 | American Home Products Corporation | 2-phenyl-1-[4-(2-aminoethoxy)-benzyl]-indoles as estrogenic agents |
US6479535B1 (en) * | 1998-05-15 | 2002-11-12 | Wyeth | 2-phenyl-1-[4-(2-aminoethoxy)-benzyl]-indole and estrogen formulations |
US7683052B2 (en) * | 2004-04-07 | 2010-03-23 | Wyeth | Crystalline polymorph of bazedoxifene acetate |
KR20060135866A (en) * | 2004-04-07 | 2006-12-29 | 와이어쓰 | Crystalline polymorph of a bazedoxifene acetate |
WO2007002823A2 (en) * | 2005-06-29 | 2007-01-04 | Wyeth | Formulations of conjugated estrogens and bazedoxifene |
-
2008
- 2008-03-27 PE PE2008000556A patent/PE20090100A1/en not_active Application Discontinuation
- 2008-03-28 JP JP2010502214A patent/JP2010523982A/en active Pending
- 2008-03-28 AU AU2008232666A patent/AU2008232666A1/en not_active Abandoned
- 2008-03-28 PA PA20088773701A patent/PA8773701A1/en unknown
- 2008-03-28 KR KR1020097022651A patent/KR20090127431A/en not_active Application Discontinuation
- 2008-03-28 CA CA002682185A patent/CA2682185A1/en not_active Abandoned
- 2008-03-28 TW TW097111357A patent/TW200902023A/en unknown
- 2008-03-28 MX MX2009010508A patent/MX2009010508A/en unknown
- 2008-03-28 BR BRPI0809950-2A2A patent/BRPI0809950A2/en not_active Application Discontinuation
- 2008-03-28 RU RU2009135267/15A patent/RU2009135267A/en unknown
- 2008-03-28 US US12/057,465 patent/US20080249303A1/en not_active Abandoned
- 2008-03-28 EP EP08744576A patent/EP2132172A1/en not_active Withdrawn
- 2008-03-28 AR ARP080101301A patent/AR065875A1/en unknown
- 2008-03-28 CN CN200880012145A patent/CN101657421A/en active Pending
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- 2008-03-28 WO PCT/US2008/058621 patent/WO2008121800A1/en active Application Filing
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4101511A (en) * | 1976-08-05 | 1978-07-18 | Exxon Research & Engineering Co. | Novel bisphenol derivatives and uses thereof |
EP0263902A1 (en) * | 1985-06-26 | 1988-04-20 | The Upjohn Company | Solubilisation and oxidation of somatotropin from transformed microorganisms |
WO1991006005A1 (en) * | 1989-10-19 | 1991-05-02 | Yamasa Shoyu Kabushiki Kaisha | Method of assaying d-vanillylmandelic acid, and reagent and kit therefor |
WO2000071142A1 (en) * | 1999-05-20 | 2000-11-30 | Alpha Therapeutic Corporation | Method for preparing dual virally inactivated immune globulin for intravenous administration |
WO2003087796A1 (en) * | 2002-04-10 | 2003-10-23 | Bristol-Myers Squibb Company | High throughput x-ray diffraction filter sample holder |
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CL2008000922A1 (en) | 2009-01-16 |
PE20090100A1 (en) | 2009-02-26 |
AU2008232666A1 (en) | 2008-10-09 |
US20080249303A1 (en) | 2008-10-09 |
AR065875A1 (en) | 2009-07-08 |
MX2009010508A (en) | 2009-10-19 |
CA2682185A1 (en) | 2008-10-09 |
PA8773701A1 (en) | 2008-11-19 |
RU2009135267A (en) | 2011-05-10 |
TW200902023A (en) | 2009-01-16 |
BRPI0809950A2 (en) | 2014-10-07 |
KR20090127431A (en) | 2009-12-11 |
CN101657421A (en) | 2010-02-24 |
EP2132172A1 (en) | 2009-12-16 |
JP2010523982A (en) | 2010-07-15 |
IL201043A0 (en) | 2010-05-17 |
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