WO2008108508A1 - Adenosine derivatives, method for the synthesis thereof, and the pharmaceutical compositions for the prevention and treatment of the inflammatory diseases containing the same as an active ingredient - Google Patents
Adenosine derivatives, method for the synthesis thereof, and the pharmaceutical compositions for the prevention and treatment of the inflammatory diseases containing the same as an active ingredient Download PDFInfo
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- WO2008108508A1 WO2008108508A1 PCT/KR2007/001131 KR2007001131W WO2008108508A1 WO 2008108508 A1 WO2008108508 A1 WO 2008108508A1 KR 2007001131 W KR2007001131 W KR 2007001131W WO 2008108508 A1 WO2008108508 A1 WO 2008108508A1
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- 0 *c(nc1N2C(*3)C4=C3C2C4)nc2c1nc[n]2[C@](*C=C1O)C1O Chemical compound *c(nc1N2C(*3)C4=C3C2C4)nc2c1nc[n]2[C@](*C=C1O)C1O 0.000 description 2
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D473/00—Heterocyclic compounds containing purine ring systems
- C07D473/26—Heterocyclic compounds containing purine ring systems with an oxygen, sulphur, or nitrogen atom directly attached in position 2 or 6, but not in both
- C07D473/32—Nitrogen atom
- C07D473/34—Nitrogen atom attached in position 6, e.g. adenine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
Definitions
- the present invention relates to adenosine derivatives. More particularly, the present invention relates to adenosine derivatives and pharmaceutically acceptable salts thereof which show high binding affinity and selectivity for specific A 3 adenosine receptors. Also, the present invention relates to a method for synthesizing the adenosine derivatives and a pharmaceutical composition for the prevention and treatment of inflammatory diseases, comprising the adenosine derivatives or pharmaceutically acceptable salts thereof.
- Adenosine is a ligand which regulates cell signaling, which accounts for various physiological functions through specific adenosine receptors located in the cell membrane.
- Adenosine an extracellular substance, acts as a neurotransmitter in a variety of physical systems, typically functioning to compensate for overactivity of certain organs and protect the body from the harmful effects of stress (Jacobson, K. A. et al . , J. Med. Chem. , 35, 407-422, 1992) .
- These functions are based on a part of the negative feedback loop in which adenosine, formed through the dephosphorylation of endocellular or extracellular ATP
- Adenosine triphosphate decreases the cellular energy and increases oxygen supply.
- Adenosine plays an important role in maintaining the homeostasis of organs such as the brain, the heart and the kidneys.
- an adenosine agonist was proven to show neuroprotective effects and was also found to be involved in pain, recognition, exercise and sleep.
- Pl receptors are adapted for adenosine while P2 receptors are adapted for ATP, ADP, UTP and UDP.
- Pl receptors can be divided into Ai, A 2 and A 3 according to ligand affinity, distribution within the body, and functional pathway, and A 2 further into A 2A and A 2B .
- These adenosine receptors are members of the G-protein-coupled receptor family. Pharmacological functions of adenosine A x , A 2A and A 2B receptors have been revealed using various selective ligands .
- Adenosine Ai and A 2 receptor agonists most derived from adenosine, have been intensively studied for use as hypotensive agents, therapeutics for mental illness and arrhythmia, lipid metabolism suppressant (therapeutics for diabetes) and neuroprotectives.
- adenosine derivatives are distributed throughout the body, and the activation thereof is accompanied by various pharmaceutical activities. In brief, there are no compounds that are able to activate only the adenosine receptors of a desired tissue.
- the function of the adenosine A 3 receptor the most; recently identified, remains unknown, in contrast to the Ai and A 2 receptors, the functions of which are well known. Extensive research has been conducted to develop selective ligands of the adenosine A 3 receptor.
- the A 3 receptor inhibited adenylyl cyclase, an enzyme that produces cAMP from ATP. Also, when activated by agonists, the A 3 receptor was proven to mediate the activation of guanosine triphosphate-dependent phospholipase C, an enzyme which catalyzes the degradation of phosphatidyl inositol into inositol triphosphate and diacylglycerol (DAG) in the brain (Ramkumar, V. et al . , J. Biol.
- a 3 receptor agonists are known to prevent cerebral diseases, such as epilepsy, and to protect the heart as well as inhibiting the release of TNF- ⁇ (tumor necrosis factor) , an inflammation mediator, and the production of MIP-Ia, interleukin-12 and interferon-y , all acting as inflammation mediators.
- a 3 adenosine receptor causes the release of inflammation factors, such as histamine, from mast cells, bronchoconstriction, and the apoptosis of immune cells. Accordingly, A 3 adenosine antagonists have the possibility of being candidates as antiinflammatory agents and anti-asthmatics. Therefore, compounds with pharmacological selectivity are believed to be drugs useful in the treatment of various diseases, including asthma, inflammation, cerebral ischemia, heart diseases, cancer, etc.
- the nucleoside based compounds N 6 - (3-iodobenzyl) -5 '- (N- methylcarbamoyl) -adenosine (IB-MECA) and N 6 - (3-iodobenzyl) -2- chloro-5 1 - (N-methy1carbamoyl) -adenosine (CI-IB-MECA) are representative human adenosine A 3 agonists, and exhibit higher affinity and selectivity for the A 3 adenosine receptor than for the Ai and A 2 adenosine receptors .
- a 3 AR antagonists with nucleoside skeletons in contrast to non-purinergic heterocyclic antagonists, exhibit high affinity and selectivity independent of species, the applicability thereof for animal test makes the nucleoside skeleton-based A 3 AR antagonists preferred drug candidates. Accordingly, there is a need for the development of selective A 3 antagonists based on nucleoside compounds.
- nucleoside compounds must have an N- methylcarbamoyl group at position 5 of the sugar moiety and a base substituted with an arylamino group or alkylamino group at position 6 of the purine moiety for A 3 adenosine receptor agonism, as in the representative materials IB-MECA and Cl-IB- MECA. Since, since the N-methylcarbamoyl group at position 5 of the sugar moiety forms a hydrogen bond to cause a conformational change essential for the agonism of the receptors (Kim, S-K. et al., J. MoI. Graph. Model., 25, 562- 577, 2006) , compounds devoid of an N-methylcarbamoyl at position 5 of the sugar moiety are thought to be strong candidates for A 3 adenosine receptor antagonists.
- A is O or S
- R is a linear or branched Ci ⁇ Cs alkyl which is non-substituted or is independently or selectively substituted with one or more C 6 ⁇ Cio aryl groups, a benzyl which is non-substituted or is independently or selectively substituted with halogen or one or more linear or branched Ci ⁇ C 4 alkoxy groups, or a hydroxycarbonyl-substituted benzyl
- Y is H or a halogen atom.
- an A 3 adenosine receptor antagonist comprising the adenosine derivative or pharmaceutically acceptable salt thereof.
- a pharmaceutical composition for the prevention and treatment of inflammatory diseases comprising the adenosine derivative or the pharmaceutically acceptable salt thereof as an active ingredient.
- the present invention pertains to an adenosine derivative compound represented by the following Chemical formula 1, a pharmaceutically acceptable salt thereof, and a composition comprising the compound or the salt as an active ingredient.
- A is 0 or S
- R is a linear or branched Ci-C 5 alkyl which is non- substituted or is independently or selectively substituted with one or more C 6 -Ci O aryl groups, a benzyl which is non- substituted or is independently or selectively substituted with halogen or one or more linear or branched C x -C 4 alkoxy groups, or a hydroxycarbonyl-substituted benzyl; and Y is H or a halogen atom.
- A is O or S
- R is methyl, ethyl, propyl, naphthylmethyl, benzyl, benzyl independently or selectively substituted with a substituent selected from a group consisting of F, Cl, Br, I, Ci ⁇ C 3 alkoxy and combinations thereof, or toluic acid, and
- Y is H or Cl.
- A is 0 or S
- R is methyl, ethyl, 1-naphthylmethyl, benzyl, 2- chlorobenzyl, 3-fluorobenzyl, 3-chlorobenzyl, 3-bromobenzyl, 3-iodobenzyl, 2-methoxy-5-chlorobenzyl, 2-methoxybenzyl, or 3- toluic acid, and Y is H or Cl.
- novel adenosine derivatives include : 1) (2R, 3£,4S)-2-(2-chloro-6-(3-fluorobenzylamino)-9tf- purin-9-yl) tetrahydrothiophen-3, 4-diol;
- the novel adenosine derivative, represented by Chemical formula 1, in accordance with the present invention may be in the form of pharmaceutically acceptable salts .
- Useful are acid addition salts formed with a variety of pharmaceutically acceptable organic acids or inorganic acids.
- suitable organic acids include carboxylic acid, phosphonic acid, sulfonic acid, acetic acid, propionic acid, octanoic acid, decanic acid, glycolic acid, lactic acid, fumaric acid, succinic acid, adipic acid, malic acid, tartaric acid, citric acid, glutamic acid, aspartic acid, maleic acid, benzoic acid, salicylic acid, phthalic acid, phenylacetic acid, benzene sulfonic acid, 2-naphthalene sulfonic acid, methyl sulfonic acid, ethyl sulfonic acid, and dodecyl sulfonic acid.
- Suitable inorganic acids may be exemplified
- adenosine derivatives represented by Chemical formula 1 are intended to include all salts, hydrates and solvates thereof as long as they can be prepared using conventional methods, as well as pharmaceutically acceptable salts thereof.
- the present invention pertains to a method for preparing the novel adenosine derivative represented by Chemical formula 1.
- the adenosine derivative may be synthesized according to the following Scheme 1.
- the method comprises reacting a compound of Chemical formula 2 with a silylated purine compound in the presence of a Lewis acid catalyst to produce a ⁇ -anomer compound of Chemical formula 3 (Step 1) ; adding hydrochloric acid to the ⁇ -anomer compound of Chemical formula 3 to produce a diol compound of Chemical formula 4 (Step 2); and reacting the diol compound of Chemical formula 4 with an amine compound in the presence of a base as a catalyst to produce the adenosine derivative (Step 3) .
- Scheme 1 Scheme 1]
- Step 1 the synthesis of the novel adenosine derivative starts with the compound of Chemical formula 2.
- this starting material is reacted with a silylated purine compound to give the ⁇ -anomer compound of Chemical formula 3.
- Trimethylsilyl trifluoromethanesulfonate (TMSOTf) may be used as the Lewis acid catalyst.
- Dichloroethane, chloroform, acetonitrile, or dichloromethane is preferably used as the solvent in Step 1, with higher preference for dichloroethane.
- the silylated purine compound can be obtained by reaction between the purine compound of Chemical formula 5 and hexamethyldisilazane (HMDS) in the presence of ammonium sulfate as a catalyst.
- HMDS hexamethyldisilazane
- Step 2 acetonide is removed from the compound of Chemical formula 3 with HCl to give a diol compound of Chemical formula 4.
- HCl acetic acid, sulfuric acid or p-toluene sulfonic acid may be used.
- Step 3 the diol compound of Chemical formula 4 obtained in Step 2 is reacted with an amine compound in the presence of a base as a catalyst to give the adenosine derivative.
- Examples of the base catalyst useful in Step 3 include triethylamine, pyridine, N,N-dimethylaminopyridine, and 1,4- dioxane with preference for triethylamine.
- the reaction may be preferably conducted in a solvent selected from among lower alcohols such as methanol and ethanol, 1,4- dioxane, tetrahydrofuran and chloroform.
- the compound of Chemical formula 2 used as the starting material for the synthesis of the adenosine derivative according to the present invention may be synthesized through the reaction route of either Scheme 2 or 3.
- Scheme 2 is taken for the synthesis of the starting material.
- the synthesis of the starting material is accomplished by reacting the D-mannose compound of Chemical formula 6 with 2, 2-dimethoxypropane in the presence of an acid as a catalyst to give the diacetonide compound of Chemical formula 7 (Step ai) ; opening the compound of Chemical formula 7 obtained in Step ai in the presence of a reducing agent to afford the diol compound of Chemical formula 8 (Step a 2 ) ; mesylating the diol compound of Chemical formula 8 obtained in Step a 2 to afford the dimesyl compound of Chemical formula 9 (Step a 3 ) ; cyclizing the compound of Chemical formula 9 obtained in Step a 3 to afford the thiosugar compound of Chemical formula 10 (Step a 4 ) ; selectively hydrolyzing the compound of Chemical formula 10 obtained in Step a 4 to afford the diol compound of Chemical formula 11 (Step as) ; and
- Step a ⁇ the synthesis of Compound 2 starts from the D-mannose of Chemical formula 6.
- D-Mannose is reacted with 2, 2-dimethoxypropane in the presence of an acid as a catalyst to give diacetonide compound of Chemical formula 7.
- Chemical formula 6 into the compound of Chemical formula 7 may be an inorganic acid, such as cone, sulfuric acid or hydrochloric acid, or an organic acid, such as p- toluenesulfonic acid.
- Step a 2r the compound of Chemical formula 7 is ring- opened in the presence of a reducing agent to afford the diol compound of Chemical formula 8.
- Chemical formula 8 In lieu of sodium borohydride, a metal hydride, such as lithium aluminum hydride, or sodium sulfite may be used.
- Step a 3 the compound of Chemical formula 8 obtained in Step a 2 is mesylated into the dimesyl compound of Chemical formula 9.
- the mesylation of the compound of Chemical formula 8 with methanesulfonylchloride (MsCl) into the compound of Chemical formula 9 is preferably conducted in an inert solvent, such as ethyl ether, petroleum ether, dichloromethane, tetrahydrofuran and N,N- dimethylformamideformamide.
- an inert solvent such as ethyl ether, petroleum ether, dichloromethane, tetrahydrofuran and N,N- dimethylformamideformamide.
- Step a 4 the compound of Chemical formula 9 obtained in Step a 3 is cyclized into a thiosugar compound of Chemical formula 10.
- the compound of Chemical formula 10 can be obtained by reacting the compound of Chemical formula 9 with sodium sulfide.
- the compound of Chemical formula 10 can be achieved by substitution with a thio ester, such as methyl thioacetate, followed by reaction with sodium alkoxide.
- N, N-dimethylformamide or dimethylsulfoxide may be used as the solvent for Step a 4 .
- Step a 5 the compound of Chemical formula 10 obtained in Step a 4 is selectively hydrolyzed into the diol compound of Chemical formula 11.
- acetic acid For the hydrolysis of the compound of Chemical formula 10 into the compound of Chemical formula 11, acetic acid may be used. In place of acetic acid, sulfuric acid, hydrochloric acid or p-toluene sulfonic acid may be used.
- Step a 6 the compound of Chemical formula 11 obtained in Step as is converted into the acetate compound of Chemical formula 2a in the presence of a catalyst.
- Scheme 3 is taken for the synthesis of the starting material 2.
- the synthesis of the starting material is accomplished by reacting the compound of Chemical formula 12 with a reducing agent to afford the lactol compound of Chemical formula 13 (Step bi) ; and reacting the compound of Chemical formula 13 obtained in Step bi with anhydrous acetic acid to afford an acetate compound of Chemical formula 2b (Step b 2 ) .
- Step bi the compound of Chemical formula 12 is reduced into the lactol compound of Chemical formula 13.
- DIBAL diisobutylammonium hydride
- Step b 2 the compound of Chemical formula 13 is reacted with anhydrous acetate to afford the acetate compound of Chemical formula 2b.
- the compound of Chemical, formula 2b can be obtained by reacting the lactol compound of Chemical formula 13 with acetate.
- the present invention pertains to an A 3 adenosine receptor antagonist comprising the adenosine derivative represented by Chemical formula 1 or a pharmaceutically acceptable salt thereof as an active ingredient.
- the present invention pertains to a pharmaceutical composition for the prevention and treatment of inflammatory diseases, comprising the adenosine derivative represented by Chemical formula 1 or a pharmaceutically acceptable salt thereof as an active ingredient.
- a 3 adenosine receptors When expressed in Chinese Hamster Ovary (CHO) cells, A 3 adenosine receptors were found to inhibit adenylyl cyclase, an enzyme that produces cAMP from ATP. Also, when activated by agonists, the A 3 adenosine receptor was proven to mediate the activation of guanosine triphosphate-dependent phospholipase C, an enzyme which catalyzes the degradation of phosphatidyl inositol into inositol triphosphate and DAG in the brain (Ramkumar, V. et al . , J. Biol. Chem. , 268, 168871-168890, 1993; Abbracchio, M. P. et al .
- a 3 receptor agonists are known to prevent cerebral diseases, such as epilepsy, and to protect the heart as well as inhibiting the release of TNF- ⁇ (tumor necrosis factor) , an inflammation mediator, and the production of MIP-Ia, interleukin-12 and interferon- Y , all of which act as inflammation mediators.
- a 3 adenosine receptor causes the release of inflammation factors, such as histamine, from mast cells, bronchoconstriction, and the apoptosis of immune cells. Accordingly, A 3 adenosine antagonists have the possibility of being candidates as anti-inflammatory agents and antiasthmatics.
- the adenosine derivatives of the present invention were assayed for human adenosine receptor (hAR) -binding affinity and selectivity.
- hAR human adenosine receptor
- the adenosine derivatives of the present invention were found to have high binding affinity for human A 3 adenosine receptors (hA 3 AR) , but low affinity for Ai and A 2 A adenosine receptors, thereby showing high selectivity.
- the compounds of Examples 15 and 16 both adenosine derivatives in the form of 4'-0 oxonucleoside show high binding affinity and selectivity (see Table 1) .
- the compounds of Examples 2 to 4 When administered to mice treated with TPA in the ears, the compounds of Examples 2 to 4, diluted in acetone, were observed to decrease inflammation of the ears to some degree (see FIG. 2) . In addition, the compounds of Examples 1 and 6 were found to have anti-inflammatory activity four or more times that of the compounds of Examples 2 to 4, as measured on the basis of inhibition percentage (FIG. 3) .
- the adenosine derivatives of the present invention were proven to have anti-inflammatory activity.
- the adenosine derivatives represented by Chemical formula 1, according to the present invention, can be effectively used as A 3 adenosine receptor antagonists. Further, the adenosine derivatives of the present invention exert antagonism on A 3 adenosine receptors, showing anti-inflammatory activity, and thus are useful in the prevention and treatment of inflammatory diseases.
- the inflammatory disease to which the adenosine derivatives of the present invention can be effectively applied include acute and chronic inflammatory diseases, such as ulcerative inflammation, exudative inflammation, purulent inflammation, hemorrhagic inflammation, and hyperplastic inflammation.
- acute and chronic inflammatory diseases such as ulcerative inflammation, exudative inflammation, purulent inflammation, hemorrhagic inflammation, and hyperplastic inflammation.
- pharmaceutical compositions comprising the adenosine derivative of the present invention or pharmaceutically acceptable salts thereof, they are formulated into dosage forms with expedients, as will be explained with the following examples, which are illustrative only, and are not intended to limit the present invention.
- the compositions of the present invention may be administered systemically or topically.
- the compound of the present invention may be clinically administered in oral or non-oral forms. It is usually formuated in combination with a diluent or excipient, such as a filler, a thickening agent, a binder, a wetting agent, a disintegrant, a surfactant, etc.
- a diluent or excipient such as a filler, a thickening agent, a binder, a wetting agent, a disintegrant, a surfactant, etc.
- Solid agents intended for oral administration of the compound of the present invention may be in the form of tablets, pills, powders, granules, capsules, and the like. These solid agents are formulated in combination with at least one excipient such as starch, calcium carbonate, sucrose, lactose, or gelatine.
- a lubricant such as magnesium stearate, talc and the like, may be added, as well .
- Liquid agents intended for oral administration include suspensions, internal use solutions, emulsion, syrups, and the like.
- various excipients such as wetting agents, sweetening agents, aromatics, preservatives, and the like may be contained in the liquid agents for the oral administration of the compound of the present invention.
- non-oral dosage forms of the compound of the present invention include injections, emulsions, inhalations, and suppositories.
- injections sterile aqueous solutions, non-aqueous solvents, and suspensions made from propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and esters such as ethylolate may be used.
- the basic materials of suppositories include witepsol, macrogol, tween 61, cacao paper, laurin paper, glycerol, and gelatine.
- the compound of the present invention may be formulated into ointments or cream for topical application.
- the administration dose of the compound of the present invention to humans may vary.
- the compound of the present invention is administered at a dose from 0.001 to 100 mg per kg of body weight a day and preferably at a dose from 0.01 to 30 mg per kg of body weight a day.
- the compound may be administered in a single dose or in divided doses per day.
- the compound of the present invention is contained in an amount from 0.0001 to 10% by weight based on the total weight of the composition and preferably in an amount from 0.001 to 1% by weight.
- the administration route is dependent on patient's health state and disease severity. Description of Drawings
- FIG. 1 is a graph showing the antagonist effect of the compound of Example 4 on the CHO cells treated with the agonist Cl-IB-MECA;
- FIG. 2 is a graph showing the anti-inflammatory activity of the compounds of the present invention (Examples 2, 3 and 4) in animal tests;
- FIG. 3 is a graph showing the anti-inflammatory activity of the compounds of the present invention (Examples 1 and 6) in animal tests;
- FIG. 4 is a graph showing the anti-inflammatory activity of the compounds of the present invention (Examples 5, 7 and 8) in animal tests; and FIG. 5 is a graph showing the anti-inflammatory activity of the compounds of the present invention (Examples 15 and 16) in animal tests .
- Step ai Preparation of ⁇ 3aR, AR, 6R, 6aR) -6- (2, 2-dimethyl- 1, 3-dioxolan4 ⁇ yl) -2, 2-dimethyl-tetrahydrofuro [3,4- d] [l,3]dioxol-4-ol
- Step a 2 Preparation of (IJ?) - (2, 2-dimethyl-l, 3- dioxolan4-yl) ( (4R, 5S) -5- (hydroxymethyl) -2, 2-dimethyl-l, 3- dioxolan.4-yl ) methanol
- the concentrate was extracted with ethylacetate and water. The organic layer was dried over anhydrous magnesium sulfate (MgSO 4 ) , filtered and concentrated in a vacuum. The concentrate was purified by silica gel column chromatography using a mixture of hexane: ethylacetate (1:1, v/v) as an elution solvent to afford the object compound in a syrup form (1.38 g, 92%).
- Step a 3 Preparation of (1.R) - (2, 2-dimethyl-l, 3- dioxolan4-yl) ( (AS, 5S) -2, 2-dimethyl-5- ( (methylsulfonyloxy) methyl) -1, 3-dioxolan4 ⁇ yl) methylmethanesulfonate
- Step a 4 Preparation of ⁇ 3aR, 45, 6aS) -4- (2, 2-dimethyl- 1, 3-dioxolan4 ⁇ yl) -2, 2-dimethyltetrahydrothieno [3, 4- d] [l,3]dioxol
- the organic layer was dried over anhydrous magnesium sulfate (MgSO 4 ) , filtered, and concentrated in a vacuum.
- the concentrate was purified through silica gel column chromatography using a mixture of hexane:ethylacetate (8:1, v/v) as an elution solvent to afford the object compound in a syrup form (453.0 mg, 78%) .
- Step a 5 Preparation of 1- ( (3aR, 4S, 6aS) -2, 2- dimethyltetrahydrothieno [3, 4-d] [1,3] dioxol-4-yl) ethan-1, 2-diol
- reaction mixture was concentrated in a vacuum and the concentrate was purified through silica gel column chromatography using a mixture of hexane : ethylacetate (1:2, v/v) as an elution solvent to afford the object compound as a white solid (14.85 g, 81%).
- Step a Preparation of (3aR, 4R,6aS) -2, 2- dimethyltetrahydrothieno [3, 4-d] [1, 3] dioxol-4-yl acetate 1- ( ⁇ 3aR, AS, ⁇ aS) -2, 2-dimethyltetrahydrothieno [3, 4- d] [l,3]dioxol-4-yl)ethan-l,2-diol (14.85 g, 67.41 iranol) , prepared in Step a 5 , was dissolved in ethylacetate (300 ml) and cooled to 0 0 C.
- the concentrate was purified through silica gel column chromatography using a mixture of hexane: ethylacetate (8:1, v/v) as an elution solvent to afford the object compound in a syrup form (8.82 g, 60%) .
- Step bj Preparation (3aR, 4R, 6aR) -2, 2-dimethyl- tetrahydrofuro [3, 4-d] [1, 3] dioxol-4-ol 2, 3-0-isopropylidene-D-erythronolactone (1.04 g, 6.42 mmol) was dissolved in toluene (20 ml) , followed by the addition of 1 M diisobutyla ⁇ imonium hydride (DIBAL) /THF to the solution at -78 0 C. The reaction mixture was stirred at the same temperature for 30 min and methanol was slowly added until the reaction terminated.
- DIBAL diisobutyla ⁇ imonium hydride
- Step b 2 Preparation of (3aS, 4S, 6a5) -2, 2-dimethyl- tetrahydrofuro[3, 4-d] [1, 3] dioxol-4-yl acetate
- the reaction mixture was cooled, diluted in dichloromethane and washed with a saturated aqueous sodium hydrogen carbonate (NaHCO 3 ) solution.
- the organic solvent was dried over anhydrous magnesium sulfate (MgSO 4 ) and concentrated in a vacuum to give a residue in the form of a yellow syrup.
- the residue was purified through silica gel column chromatography using a mixture of dichloromethane :methanol (50 : 1, v/v) as an elution solvent to afford the object compound in the form of a foam (3.03 g, 79%) .
- Step 3 Preparation of [2R, 3R 1 4S) -2- (2-chloro-6- (3- iodobenzylamino) -9H-purin-9-yl) tetrahydrothiophen-3, 4-diol
- 3-iodobenzylamine was used instead of 3-fluorobenzylamine, to give the object compound (0.14 g, 84%) .
- Step 1 Preparation of 2, 6-dichloro-9- ( ⁇ 3aR, 4R 1 6aS) -2, 2- dimethyltetrahydrothieno [3, 4-d] [1,3] dioxol-4-yl) -9H-purine
- Step 1 Preparation of 2, 6-dichloro-9- ( ⁇ 3aR, 4R 1 6aS) -2, 2- dimethyltetrahydrothieno [3, 4-d] [1,3] dioxol-4-yl) -9H-purine
- Step 2 Preparation of ⁇ 2R, 35, 4S) -2- (2, 6-dichloro-9H- purin-9-yl) tetrahydrothiophen-3, 4-diol
- Step 2 The same procedure as in Step 2 of Example 1 was conducted to give the object compound as a white solid.
- Step 3 Preparation of (2R, 3R, 4S) -2- (2-chloro-6- (2- chlorobenzylamino) -9H-purin-9-yl) tetrahydrothiophen-3, 4-diol
- 2-chlorobenzylamine was used instead of 3- fluorobenzylamine, was conducted to give the object compound (0.11 g, 81%) . m.p. 198.7-199.7°C; UV (MeOH) ⁇ max 273.5 nm;
- Step 2 Preparation of (2R,3S, AS) -2- (2, 6-dichloro-9H ⁇ purin-9-yl) tetrahydrothiophen-3, 4-diol
- Step 3 Preparation of 2- (2-chloro-6-methylamino-purin ⁇ 9-yl) (2.R, 35, 4.R) -tetrahydrothiophen-3, 4-diol
- Step 2 Preparation of [2R 1 35, 45) -2- (6-chloro-9ff-purin- 9-yl) tetrahydrothiophen-3, 4-diol Synthesis was conducted from 6-chloro-9- ( (3a.R, 4R, 6aS) - 2, 2-dimethyltetrahydrothieno [3, 4-d] [1,3] dioxol-4-yl) -9/f-purine (1.84 g, 5.88 mmol), prepared in Step 1, in a manner similar to that of Step 2 of Example 1 to afford the object compound as a white solid (1.27 g, 79%). m.p. 192.3-192.8°C;
- Step 1 Preparation of 6-chloro-9- ( (3a.R, 4,R, 6a5) -2, 2- dimethyltetrahydrothieno [3, 4-d] [1,3] dioxol-4-yl) -9J ⁇ -purine
- Step 1 of Example 11 The same procedure as in Step 1 of Example 11 was conducted to give the object compound in foam form.
- Step 2 Preparation of ⁇ 2R, 3R, AR) -2- ( 2 , 6-dichloro- 9ff- purin-9-yl) tetrahydrofuro-3, 4-diol
- the adenosine derivatives of the present invention were assayed for binding affinity for human Ai, A 2a and A 3 adenosine receptors as follows.
- CHO cells (ATCC No. CCL-61), in which A 1 and A 3 adenosine receptors were expressed, were cultured in F-12 media (Gibco, U.S.A.) supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin (100 units/ml and 100 ⁇ g/ml), at 37°C in a 5% CO 2 atmosphere.
- F-12 media Gibco, U.S.A.
- FBS fetal bovine serum
- penicillin/streptomycin 100 units/ml and 100 ⁇ g/ml
- a predetermined amount of suitable hAR-expressed CHO cells was mixed with labeled ligands (1 nM [ 3 H]CCPA and 0.5 nM [ 125 I]AB-MECA) specifically binding to A 1 and A 3 adenosine receptors in a 50/10/1 buffer in test tubes .
- the derivatives of the present invention were dissolved at various concentrations in dimethylsulfoxide (DMSO) and diluted in the buffer, taking care that the final concentration of DMSO did not exceed 1%. Incubation for 1 hr in a 37 0 C incubator was followed by rapid filtration in a vacuum using a cell collector (TOMTEC, U.S.A.). Subsequently, the test tubes were washed three times with 3 ml of the buffer before radioactivity was measured using a ⁇ -counter.
- DMSO dimethylsulfoxide
- the equilibrium constant K 1 for non-specific binding was determined in the presence of 10 ⁇ M of 5' -N-ethylcarboxamide adenosine (NECA) as a non-labeled ligand.
- the equilibrium constant K ⁇ was calculated according to the Cheng-Prusoff equation on the assumption that [ 125 I]AB-MECA has a K d value of 1.48 nM.
- K 1 for binding affinity was determined by subtracting the non- specific binding value from the total binding value. On the basis of the specific binding values, the samples were analyzed for binding affinity to various adenosine receptors.
- the compounds synthesized in the examples of the present invention were found to have high binding affinity for human A 3 adenosine receptors (hA 3 AR) , but low affinity for Ai and A 2A adenosine receptors, thereby showing high selectivity.
- the compounds having halobenzyl substituents were found to have binding affinity in decreasing order of Cl > I > F > Br.
- the adenosine derivatives having a substituent at the 3-position of the benzene ring in accordance with the present invention had stronger binding affinity for hA 3 AR than the adenosine derivatives, having a substituent at the 2- or 4-position, or two substituents at the 2- and 5-position.
- the compounds of Examples 15 and 16 both adenosine derivatives having 4'-0 oxonucleoside forms, also had high binding affinity and selectivity, which were, however, not superior to those of the corresponding 4'-S thionucleoside forms, such as those of Examples 3 and 4.
- an assay for antagonism and cAMP inhibition was conducted by treating CHO cells with the compound of Example 4 and Cl-IB-MECA.
- the adenosine derivatives of the present invention were examined for anti-inflammatory activity in the following animal test. Seven-week-old male ICR mice were treated with TPA (12-O-tetradecanoylphorbol 13-acetate, 20 ⁇ l) in the right ear. Within 15 minutes, the compounds of Examples 1 to 16 were diluted at a concentration of 0.5% in acetone (20 ⁇ l) , distilled water, or mixtures of DMSO and acetone (compositions shown in Tables 2 to 5) before being administered to the mice. Hydrocortisone was used at the same concentration as a control .
- mice 6 hrs after treatment with TPA, the mice were secondarily treated with the adenosine derivatives of the present invention. 24 hrs after TPA treatment, test animals were euthanized using a cervical dislocation method. Samples were obtained from the right ear using a 6 mm diameter punch. The activity was observed by measuring the ear sample using a microbalance. Percentages of inhibition were calculated using the following Equation 1. The compositions and amounts used in these experiments are summarized in Tables 2 to 5 and the anti-inflammatory activities thereof are shown in FIGS. 2 to 5.
- the compounds synthesized in the examples of the present invention were assayed for cytotoxicity in animals.
- Three test groups of three 25+5 g ICR rats (Central Experiment Animal, Korea) and three test groups of three 235 ⁇ 10 g specific pathogen-free (SPF) Sprague Dawley rats (Central Experiment Animal, Korea) were abdominally administered with the compound of Example 2 at doses of 20 mg/kg, 10 mg/kg, and 1 mg/kg, respectively, followed by observation for 24 hrs .
- adenosine compounds of the present invention may be administered in the following dosage forms and the following Formulation Examples are set forth to illustrate, but not limit, the present invention.
- the ingredients were mixed and compressed into tablets according to a conventional method.
- the pH of a solution of the active ingredient in distilled water was adjusted to 7.5 and the solution was diluted in sterile water to a volume 2 ml and loaded into ampules before sterilization.
- a liquid dosage form was prepared by dissolving the ingredients in pure water, adding a suitable amount of lemon flavor, increasing the volume to 100 ml with pure water, loading the volume into a brown vial, and sterilizing.
- the adenosine derivatives of the present invention have high binding affinity and selectivity for adenosine receptors, especially for A 3 adenosine receptors, and act as A 3 adenosine receptor antagonists, showing anti-inflammatory activity. Therefore, the adenosine derivatives of the present invention are useful in the prevention and treatment of inflammatory diseases. Examples are described in terms of the preferred embodiment of present invention. However, it should not be understood that such disclosure is not limited to explicit description of present invention. The description and the claims of present invention are to be interpreted as covering all alterations and modifications within the true scope of this invention.
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CA2680179A CA2680179C (en) | 2007-03-07 | 2007-03-07 | Adenosine derivatives, method for the synthesis thereof, and the pharmaceutical compositions for the prevention and treatment of the inflammatory diseases containing the same as an active ingredient |
PCT/KR2007/001131 WO2008108508A1 (en) | 2007-03-07 | 2007-03-07 | Adenosine derivatives, method for the synthesis thereof, and the pharmaceutical compositions for the prevention and treatment of the inflammatory diseases containing the same as an active ingredient |
CN200780052031XA CN101801970B (en) | 2007-03-07 | 2007-03-07 | Adenosine derivatives, method for the synthesis thereof, and the pharmaceutical compositions for the prevention and treatment of the inflammatory diseases containing the same as an active ingredient |
AU2007348394A AU2007348394B2 (en) | 2007-03-07 | 2007-03-07 | Adenosine derivatives, method for the synthesis thereof, and the pharmaceutical compositions for the prevention and treatment of the inflammatory diseases containing the same as an active ingredient |
US12/530,086 US9018371B2 (en) | 2007-03-07 | 2007-03-07 | Adenosine derivatives, method for the synthesis thereof, and the pharmaceutical compositions for the prevention and treatment of the inflammatory diseases containing the same as an active ingredient |
JP2009552570A JP5306238B2 (en) | 2007-03-07 | 2007-03-07 | Adenosine derivative, synthesis method thereof, and pharmaceutical composition for prevention and treatment of inflammatory disease containing the same as an active ingredient |
EP07715531.5A EP2142549B1 (en) | 2007-03-07 | 2007-03-07 | Adenosine derivatives, method for the synthesis thereof, and the pharmaceutical compositions for the prevention and treatment of the inflammatory diseases containing the same as an active ingredient |
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WO2011096642A2 (en) * | 2010-02-02 | 2011-08-11 | 이화여자대학교 산학협력단 | Pharmaceutical composition for preventing and treating inflammatory diseases |
CN111943938A (en) * | 2019-05-17 | 2020-11-17 | 上海再极医药科技有限公司 | Synthetic method of A2A adenosine receptor antagonist |
EP4186514A1 (en) * | 2021-11-24 | 2023-05-31 | Future Medicine Co., Ltd. | Pharmaceutical composition for preventing or treating cholangitis or liver disease caused by cholangitis comprising adenosine derivative |
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JP2012196333A (en) * | 2011-03-22 | 2012-10-18 | Meijo Univ | Method and device for preventing drowsy driving by elderly people |
KR101323413B1 (en) | 2012-07-26 | 2013-10-29 | 이화여자대학교 산학협력단 | A pharmaceutical composition for preventing of treating chronic kidney disease comprising (2r,3r,4s)-2-(2-chloro-6-(3-iodobenzylamino)-9h-purin-9-yl)-tetrahydrothiophene-3,4-diol or its derivatives |
EP3402798B1 (en) | 2016-01-14 | 2021-04-28 | Handok Inc. | Compounds antagonizing a3 adenosine receptor, method for preparing them, and medical-use thereof |
KR101709307B1 (en) * | 2016-10-31 | 2017-02-23 | 퓨쳐메디신 주식회사 | The pharmaceutical compositions for the prevention and treatment of the nash, liver fibrosis and liver cirrhosis containing adenosine derivatives |
KR101805400B1 (en) * | 2017-03-21 | 2017-12-07 | 퓨쳐메디신 주식회사 | The pharmaceutical compositions for the prevention and treatment of the glaucoma containing adenosine derivatives |
KR101820909B1 (en) * | 2017-07-07 | 2018-01-23 | 퓨쳐메디신 주식회사 | A pharmaceutical compositions for the prevention and treatment of chronic kidney disease containing adenosine derivatives |
IL254535A0 (en) * | 2017-09-17 | 2017-11-30 | Can Fite Biopharma Ltd | A3 adenosine receptor ligand for managing cytokine release syndrome |
KR102007640B1 (en) * | 2017-11-29 | 2019-08-07 | 퓨쳐메디신 주식회사 | The pharmaceutical compositions for the prevention and treatment of retinal diseases or optic nerve diseases containing adenosine derivatives |
CN107903274A (en) * | 2017-12-28 | 2018-04-13 | 窦玉玲 | A kind of aminated compounds and its application in antitumor drug |
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US5688744A (en) * | 1995-11-03 | 1997-11-18 | Rohm And Haas Company | Antimicrobial compounds with quick speed of kill |
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Cited By (5)
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WO2011096642A2 (en) * | 2010-02-02 | 2011-08-11 | 이화여자대학교 산학협력단 | Pharmaceutical composition for preventing and treating inflammatory diseases |
WO2011096642A3 (en) * | 2010-02-02 | 2011-11-10 | 이화여자대학교 산학협력단 | Pharmaceutical composition for preventing and treating inflammatory diseases |
KR101207755B1 (en) | 2010-02-02 | 2012-12-03 | 이화여자대학교 산학협력단 | Pharmaceutical Composition for Treating Inflammatory Disease |
CN111943938A (en) * | 2019-05-17 | 2020-11-17 | 上海再极医药科技有限公司 | Synthetic method of A2A adenosine receptor antagonist |
EP4186514A1 (en) * | 2021-11-24 | 2023-05-31 | Future Medicine Co., Ltd. | Pharmaceutical composition for preventing or treating cholangitis or liver disease caused by cholangitis comprising adenosine derivative |
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JP2010520271A (en) | 2010-06-10 |
CA2680179C (en) | 2016-02-16 |
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EP2142549A4 (en) | 2011-07-27 |
CA2680179A1 (en) | 2008-09-12 |
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