WO2008096354A3 - Means and methods of producing fruits with high levels of anthocyanins and flavonols - Google Patents

Means and methods of producing fruits with high levels of anthocyanins and flavonols Download PDF

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Publication number
WO2008096354A3
WO2008096354A3 PCT/IL2008/000159 IL2008000159W WO2008096354A3 WO 2008096354 A3 WO2008096354 A3 WO 2008096354A3 IL 2008000159 W IL2008000159 W IL 2008000159W WO 2008096354 A3 WO2008096354 A3 WO 2008096354A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
methods
disclosed
aft
flavonols
Prior art date
Application number
PCT/IL2008/000159
Other languages
French (fr)
Other versions
WO2008096354A2 (en
Inventor
Ilan Levin
Michal Oren-Shamir
Maya Sapir
Moshe Reuveni
Yaakov Tadmor
Original Assignee
Israel State
Ilan Levin
Michal Oren-Shamir
Maya Sapir
Moshe Reuveni
Yaakov Tadmor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Israel State, Ilan Levin, Michal Oren-Shamir, Maya Sapir, Moshe Reuveni, Yaakov Tadmor filed Critical Israel State
Priority to CA002677077A priority Critical patent/CA2677077A1/en
Priority to EP08710159A priority patent/EP2131646A2/en
Priority to AU2008212096A priority patent/AU2008212096A1/en
Priority to US12/526,173 priority patent/US20100199370A1/en
Priority to JP2009547812A priority patent/JP2010517526A/en
Publication of WO2008096354A2 publication Critical patent/WO2008096354A2/en
Publication of WO2008096354A3 publication Critical patent/WO2008096354A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Means and methods for providing an AFT gene encoding a protein characterized by at least 80% identity with the amino acid sequence shown in Fig. 9 (LA1996 Seq.) having been genetically introgressed into cultivated tomato plants or elite lines. The AFT gene confers higher concentrations of flavonoids to the plants compared with prior art cultivated plants that were not introgressed with the gene. An AFT S. chilense genotype introgressively-derived tomato plant is disclosed. Transgenic plants expressing metabolites of the flavonoid pathway, especially anthocyanin or flavonols, in plants, plant parts or seeds thereof, carrying particular DNA sequences recombinable into a plurality of one or more transformation and/or expression vectors, useful for transformation and/or expression in plants are disclosed. Methods of obtaining same are disclosed.
PCT/IL2008/000159 2007-02-06 2008-02-06 Means and methods of producing fruits with high levels of anthocyanins and flavonols WO2008096354A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002677077A CA2677077A1 (en) 2007-02-06 2008-02-06 Means and methods of producing fruits with high levels of anthocyanins and flavonols
EP08710159A EP2131646A2 (en) 2007-02-06 2008-02-06 Means and methods of producing fruits with high levels of anthocyanins and flavonols
AU2008212096A AU2008212096A1 (en) 2007-02-06 2008-02-06 Means and methods of producing fruits with high levels of anthocyanins and flavonols
US12/526,173 US20100199370A1 (en) 2007-02-06 2008-02-06 Means and methods of producing fruits with high levels of anthocyanins and flavonols
JP2009547812A JP2010517526A (en) 2007-02-06 2008-02-06 Means and methods for producing fruits containing large amounts of anthocyanins and flavonols

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL181193A IL181193A0 (en) 2007-02-06 2007-02-06 Means and methods of producing fruits with high levels of anthocyanins and flavonols
IL181193 2007-02-06

Publications (2)

Publication Number Publication Date
WO2008096354A2 WO2008096354A2 (en) 2008-08-14
WO2008096354A3 true WO2008096354A3 (en) 2008-11-06

Family

ID=39682185

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2008/000159 WO2008096354A2 (en) 2007-02-06 2008-02-06 Means and methods of producing fruits with high levels of anthocyanins and flavonols

Country Status (7)

Country Link
US (1) US20100199370A1 (en)
EP (1) EP2131646A2 (en)
JP (1) JP2010517526A (en)
AU (1) AU2008212096A1 (en)
CA (1) CA2677077A1 (en)
IL (1) IL181193A0 (en)
WO (1) WO2008096354A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7855319B2 (en) * 2006-07-06 2010-12-21 J.R. Simplot Company High level antioxidant-containing foods
CN101648959B (en) * 2008-08-15 2011-05-11 巴塞利亚药业（中国）有限公司 Coumaronochromones compound and preparation and application thereof
CN104693213B (en) * 2015-01-21 2017-05-24 南开大学 Synthesis method of flavonoid natural compounds Frutinones A, B and C
CA3000049A1 (en) * 2015-10-30 2017-05-04 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Tomato plant producing fruits with beneficial compounds
CN107365865B (en) * 2017-09-01 2020-12-08 中国农业大学 Molecular marker related to tomato fruit color and application thereof
CN110029117B (en) * 2019-04-04 2022-11-01 怀化学院 Flavonol synthetase gene SmFLS and application thereof, and flavonol synthetase
CN111394499B (en) * 2020-04-15 2022-04-19 四川农业大学 Nucleic acid composition for screening high-anthocyanin tea trees, application of nucleic acid composition and method for breeding high-anthocyanin tea trees
CN112159864A (en) * 2020-09-30 2021-01-01 河南科技大学 qRT-PCR reference gene of grape and application thereof
CN112831520B (en) * 2021-01-07 2022-07-12 山东农业大学 Application of R1R6 genotype apples as receptor material in preparation of red-meat transgenic apples
CN114414809B (en) * 2022-03-28 2022-06-21 中元伯瑞生物科技(珠海横琴)有限公司 Use of biomarkers for diagnosing pneumoconiosis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020133848A1 (en) * 2000-10-30 2002-09-19 Karin Connors Identification and characterization of an anthocyanin mutant (ANT1) in tomato
WO2003084312A2 (en) * 2002-04-04 2003-10-16 Exelixis Plant Sciences, Inc. Identification and characterization of an anthocyanin mutant (ant1) in tomato

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7304207B2 (en) * 2001-10-29 2007-12-04 Exelixis, Inc. Identification and characterization of an Anthocyanin mutant (ANT1) in tomato
AU2004281325B2 (en) * 2003-10-21 2009-11-26 The Volcani Center- the State of Israel, Ministry of Agriculture, Agricultural Research Organisation Isolated nucleotide sequences responsible for the tomato high pigment-1 mutant phenotypes (HP-1 and HP-1W) and uses thereof
WO2005120215A1 (en) * 2004-06-11 2005-12-22 Plant Research International B.V. The shine clade of transcription factors and their use
IL164125A (en) * 2004-09-19 2014-06-30 Arthur Schaffer Gene controlling fissure development in tomato fruit cuticles

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020133848A1 (en) * 2000-10-30 2002-09-19 Karin Connors Identification and characterization of an anthocyanin mutant (ANT1) in tomato
WO2003084312A2 (en) * 2002-04-04 2003-10-16 Exelixis Plant Sciences, Inc. Identification and characterization of an anthocyanin mutant (ant1) in tomato

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JONES C M ET AL: "Characterization and inheritance of the Anthocyanin fruit (Aft) tomato", JOURNAL OF HEREDITY, OXFORD UNIVERSITY PRESS, CARY, GB, vol. 94, no. 6, 1 November 2003 (2003-11-01), pages 449 - 456, XP009104690, ISSN: 0022-1503 *
WILLITS M G ET AL: "Utilization of the genetic resources of wild species to create a nontransgenic high flavonoid tomato", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY. WASHINGTON, US, vol. 53, no. 4, 23 February 2005 (2005-02-23), pages 1231 - 1236, XP002345788, ISSN: 0021-8561 *
YEN H C ET AL: "The tomato high-pigment (hp) locus maps to chromosome 2 and influences plastome copy number and fruit quality", THEORETICAL AND APPLIED GENETICS, SPRINGER, BERLIN, DE, vol. 95, no. 7, 1 November 1997 (1997-11-01), pages 1069 - 1079, XP002097198, ISSN: 0040-5752 *

Also Published As

Publication number Publication date
WO2008096354A2 (en) 2008-08-14
CA2677077A1 (en) 2008-08-14
AU2008212096A1 (en) 2008-08-14
IL181193A0 (en) 2007-07-04
JP2010517526A (en) 2010-05-27
US20100199370A1 (en) 2010-08-05
EP2131646A2 (en) 2009-12-16

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