WO2008087562A3 - Methods and compositions for modulating the mirna pathway - Google Patents

Methods and compositions for modulating the mirna pathway Download PDF

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Publication number
WO2008087562A3
WO2008087562A3 PCT/IB2008/000987 IB2008000987W WO2008087562A3 WO 2008087562 A3 WO2008087562 A3 WO 2008087562A3 IB 2008000987 W IB2008000987 W IB 2008000987W WO 2008087562 A3 WO2008087562 A3 WO 2008087562A3
Authority
WO
WIPO (PCT)
Prior art keywords
mirna
modulating
compositions
methods
virulent
Prior art date
Application number
PCT/IB2008/000987
Other languages
French (fr)
Other versions
WO2008087562A9 (en
WO2008087562A8 (en
WO2008087562A2 (en
Inventor
Lionel Navarro
Oliver Voinnet
Original Assignee
Plant Bioscience Ltd Pbl
Lionel Navarro
Oliver Voinnet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Bioscience Ltd Pbl, Lionel Navarro, Oliver Voinnet filed Critical Plant Bioscience Ltd Pbl
Priority to CA002675954A priority Critical patent/CA2675954A1/en
Priority to EP08750854A priority patent/EP2111457A2/en
Priority to US12/523,650 priority patent/US20100125919A1/en
Priority to BRPI0806742-2A priority patent/BRPI0806742A2/en
Publication of WO2008087562A2 publication Critical patent/WO2008087562A2/en
Publication of WO2008087562A8 publication Critical patent/WO2008087562A8/en
Publication of WO2008087562A3 publication Critical patent/WO2008087562A3/en
Publication of WO2008087562A9 publication Critical patent/WO2008087562A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function

Abstract

A specific spectrum of plant and animal miRNAs confer enhanced resistance to virulent pathogens. Plants deficient in miRNA accumulation are hyper-susceptible to non-virulent bacterial pathogens, and virulent bacteria suppress miRNA pathways by means of injected type-Ill secreted (TTS) proteins. Methods and compositions for modulating the miRNA pathway in plants and animals are disclosed whereby adverse effects on plant and animal development are avoided or minimized.
PCT/IB2008/000987 2007-01-18 2008-01-18 Methods and compositions for modulating the mirna pathway WO2008087562A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002675954A CA2675954A1 (en) 2007-01-18 2008-01-18 Methods and compositions for modulating the mirna pathway
EP08750854A EP2111457A2 (en) 2007-01-18 2008-01-18 Methods and compositions for modulating the mirna pathway
US12/523,650 US20100125919A1 (en) 2007-01-18 2008-01-18 Methods and compositions for modulating the mirna pathway
BRPI0806742-2A BRPI0806742A2 (en) 2007-01-18 2008-01-18 methods and compositions for modulating the mirna pathway

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US88143307P 2007-01-18 2007-01-18
US88143407P 2007-01-18 2007-01-18
US60/881,434 2007-01-18
US60/881,433 2007-01-18

Publications (4)

Publication Number Publication Date
WO2008087562A2 WO2008087562A2 (en) 2008-07-24
WO2008087562A8 WO2008087562A8 (en) 2008-10-09
WO2008087562A3 true WO2008087562A3 (en) 2009-03-05
WO2008087562A9 WO2008087562A9 (en) 2009-05-28

Family

ID=39636445

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/000987 WO2008087562A2 (en) 2007-01-18 2008-01-18 Methods and compositions for modulating the mirna pathway

Country Status (5)

Country Link
US (1) US20100125919A1 (en)
EP (3) EP2111457A2 (en)
BR (1) BRPI0806742A2 (en)
CA (1) CA2675954A1 (en)
WO (1) WO2008087562A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140356961A1 (en) * 2011-07-22 2014-12-04 University Of Delaware Technology Park Use of phased small rnas for suppression of plant defense and other plant genes
WO2013055898A1 (en) * 2011-10-14 2013-04-18 The Trustees Of The University Of Pennsylvania Methods for enhancing the efficacy of microrna mediated interference
US10822615B2 (en) 2011-12-27 2020-11-03 Commonwealth Scientific And Industrial Research Organisation Simultaneous gene silencing and suppressing gene silencing in the same cell
CN103194484B (en) * 2012-01-09 2015-05-27 中国科学院微生物研究所 Disease resistant rice cultivating method, and special vector and promoter therefor
WO2015048083A1 (en) * 2013-09-24 2015-04-02 The Feinstein Institute For Medical Research Peptides inhibiting cold-inducible rna binding protein activity
WO2016096923A1 (en) * 2014-12-15 2016-06-23 Paris Sciences Et Lettres - Quartier Latin Methods to monitor post-transcriptional gene silencing activity in plant tissues/cell types relevant for pathogen entry, propagation or replication
CA3226019A1 (en) 2021-07-20 2023-01-26 Ags Therapeutics Sas Extracellular vesicles from microalgae, their preparation, and uses
WO2023144127A1 (en) 2022-01-31 2023-08-03 Ags Therapeutics Sas Extracellular vesicles from microalgae, their biodistribution upon administration, and uses
WO2024088808A1 (en) 2022-10-24 2024-05-02 Ags Therapeutics Sas Extracellular vesicles from microalgae, their biodistribution upon intranasal administration, and uses thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030204868A1 (en) * 2002-02-12 2003-10-30 Alan Collmer Pseudomonas avr and hop proteins, their encoding nucleic acids, and use thereof
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
WO2005005634A2 (en) * 2003-07-09 2005-01-20 Università Degli Studi Di Roma La Sapienza siRNA EXPRESSION SYSTEM
US20050144669A1 (en) * 2003-07-01 2005-06-30 Whitehead Institute For Biomedical Research MicroRNAs in plants
WO2006044322A2 (en) * 2004-10-12 2006-04-27 The Rockefeller University Micrornas

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004522406A (en) * 2000-04-03 2004-07-29 コーネル リサーチ ファンデーション インコーポレーテッド DNA molecules and polypeptides of Pseudomonas syringae Hrp pathogenic islets and uses thereof
WO2004016745A2 (en) * 2002-08-16 2004-02-26 Boyce Thompson Institute For Plant Research Backterial effector proteins which inhibit programmed cell death

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030204868A1 (en) * 2002-02-12 2003-10-30 Alan Collmer Pseudomonas avr and hop proteins, their encoding nucleic acids, and use thereof
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
US20050144669A1 (en) * 2003-07-01 2005-06-30 Whitehead Institute For Biomedical Research MicroRNAs in plants
WO2005005634A2 (en) * 2003-07-09 2005-01-20 Università Degli Studi Di Roma La Sapienza siRNA EXPRESSION SYSTEM
WO2006044322A2 (en) * 2004-10-12 2006-04-27 The Rockefeller University Micrornas

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NAVARRO LIONEL ET AL: "A plant miRNA contributes to antibacterial resistance by repressing auxin signaling", SCIENCE (WASHINGTON D C), vol. 312, no. 5772, April 2006 (2006-04-01), pages 436 - 439, XP002494828, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
US20100125919A1 (en) 2010-05-20
WO2008087562A9 (en) 2009-05-28
WO2008087562A8 (en) 2008-10-09
CA2675954A1 (en) 2008-07-24
EP2666865A2 (en) 2013-11-27
BRPI0806742A2 (en) 2011-09-13
WO2008087562A2 (en) 2008-07-24
EP2687604A2 (en) 2014-01-22
EP2687604A3 (en) 2014-04-16
EP2111457A2 (en) 2009-10-28
EP2666865A3 (en) 2014-02-19

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